UHPLC-ESI Analysis and Antifungal Activity of Cladodes and Callus Cultures of Cereus hildmannianus

Plant biotechnology has a great potential in providing bioactive compounds with pharmacological applications. Bioactive compounds have been highlighted as an alternative antifungal treatment option due to the limited number of antifungals currently available. The aim of this study was to evaluate th...
Ausführliche Beschreibung

Gespeichert in:

Autor*in:	da Silva Santos, Éverton [verfasserIn] Savam, Aline Arita, Glaucia Sayuri Cabral, Márcia Regina Pereira Bonfim-Mendonça, Patrícia Souza de Fatima Pires Silva Machado, Maria de Oliveira, Arildo José Braz Gonçalves, Regina Aparecida Correia

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2023

Schlagwörter:	Candidiasis Cactaceae Cactus Phenethylamines Tetrahydroisoquinoline Tissue culture

Anmerkung:	© The Author(s) under exclusive licence to Sociedade Brasileira de Farmacognosia 2023

Übergeordnetes Werk:	Enthalten in: Revista Brasileira de farmacognosia - Critiba : Soc. Brasileira de Farmacognosia, 1986, 33(2023), 5 vom: 03. Juli, Seite 1072-1077
Übergeordnetes Werk:	volume:33 ; year:2023 ; number:5 ; day:03 ; month:07 ; pages:1072-1077

Links:	Volltext

DOI / URN:	10.1007/s43450-023-00418-2

Katalog-ID:	SPR052933156

Internformat


LEADER	01000naa a22002652 4500
001	SPR052933156
003	DE-627
005	20230831064742.0
007	cr uuu---uuuuu
008	230831s2023 xx \|\|\|\|\|o 00\| \|\|eng c
024	7		\|a 10.1007/s43450-023-00418-2 \|2 doi
035			\|a (DE-627)SPR052933156
035			\|a (SPR)s43450-023-00418-2-e
040			\|a DE-627 \|b ger \|c DE-627 \|e rakwb
041			\|a eng
100	1		\|a da Silva Santos, Éverton \|e verfasserin \|0 (orcid)0000-0003-2400-2898 \|4 aut
245	1	0	\|a UHPLC-ESI Analysis and Antifungal Activity of Cladodes and Callus Cultures of Cereus hildmannianus
264		1	\|c 2023
336			\|a Text \|b txt \|2 rdacontent
337			\|a Computermedien \|b c \|2 rdamedia
338			\|a Online-Ressource \|b cr \|2 rdacarrier
500			\|a © The Author(s) under exclusive licence to Sociedade Brasileira de Farmacognosia 2023
520			\|a Plant biotechnology has a great potential in providing bioactive compounds with pharmacological applications. Bioactive compounds have been highlighted as an alternative antifungal treatment option due to the limited number of antifungals currently available. The aim of this study was to evaluate the antifungal activity and chemical profile of Cereus hildmannianus K.Schum., Cactaceae, cladodes and callus cultures. The cladodes of wild plants were collected, and the conventional callus was cultivated using agar and Murashige and Skoog medium, while the alternative culture was cultivated using a cotton support covered with filter paper and the same medium (incubated at 32 °C with 16-h photoperiod). Ethanolic extracts were prepared by Soxhlet and chemically characterized by ultra-high-performance liquid chromatography coupled with electrospray ionization quadrupole time-of-flight tandem mass spectrometry (UHPLC-ESI-QToF-MS/MS). The antifungal activity was evaluated against Candida glabrata, C. parapsilosis, C. tropicalis, and C. albicans by broth microdilution. The UHPLC-ESI-QToF-MS/MS method enabled the putative identification of fifteen proto-alkaloids and alkaloids, with pellotine and mescaline described for the first time in this species. The antifungal activity of each extract was species-dependent; extracts from both the conventional and alternative callus cultures were prominent against C. glabrata (minimal inhibitory concentration of 312 µg/ml), but there was also potential inhibitory activity against other Candida species. Graphical Abstract
650		4	\|a Candidiasis \|7 (dpeaa)DE-He213
650		4	\|a Cactaceae \|7 (dpeaa)DE-He213
650		4	\|a Cactus \|7 (dpeaa)DE-He213
650		4	\|a Phenethylamines \|7 (dpeaa)DE-He213
650		4	\|a Tetrahydroisoquinoline \|7 (dpeaa)DE-He213
650		4	\|a Tissue culture \|7 (dpeaa)DE-He213
700	1		\|a Savam, Aline \|0 (orcid)0000-0003-2616-8816 \|4 aut
700	1		\|a Arita, Glaucia Sayuri \|0 (orcid)0000-0002-0334-7566 \|4 aut
700	1		\|a Cabral, Márcia Regina Pereira \|0 (orcid)0000-0002-4093-8808 \|4 aut
700	1		\|a Bonfim-Mendonça, Patrícia Souza \|0 (orcid)0000-0002-0116-2189 \|4 aut
700	1		\|a de Fatima Pires Silva Machado, Maria \|0 (orcid)0000-0001-7815-4606 \|4 aut
700	1		\|a de Oliveira, Arildo José Braz \|0 (orcid)0000-0001-8737-0546 \|4 aut
700	1		\|a Gonçalves, Regina Aparecida Correia \|0 (orcid)0000-0003-4070-1269 \|4 aut
773	0	8	\|i Enthalten in \|t Revista Brasileira de farmacognosia \|d Critiba : Soc. Brasileira de Farmacognosia, 1986 \|g 33(2023), 5 vom: 03. Juli, Seite 1072-1077 \|w (DE-627)730275531 \|w (DE-600)2690840-2 \|x 1981-528X \|7 nnns
773	1	8	\|g volume:33 \|g year:2023 \|g number:5 \|g day:03 \|g month:07 \|g pages:1072-1077
856	4	0	\|u https://dx.doi.org/10.1007/s43450-023-00418-2 \|z lizenzpflichtig \|3 Volltext
912			\|a GBV_USEFLAG_A
912			\|a SYSFLAG_A
912			\|a GBV_SPRINGER
912			\|a GBV_ILN_11
912			\|a GBV_ILN_20
912			\|a GBV_ILN_22
912			\|a GBV_ILN_23
912			\|a GBV_ILN_24
912			\|a GBV_ILN_31
912			\|a GBV_ILN_32
912			\|a GBV_ILN_39
912			\|a GBV_ILN_40
912			\|a GBV_ILN_60
912			\|a GBV_ILN_62
912			\|a GBV_ILN_63
912			\|a GBV_ILN_65
912			\|a GBV_ILN_69
912			\|a GBV_ILN_70
912			\|a GBV_ILN_73
912			\|a GBV_ILN_74
912			\|a GBV_ILN_90
912			\|a GBV_ILN_95
912			\|a GBV_ILN_100
912			\|a GBV_ILN_101
912			\|a GBV_ILN_105
912			\|a GBV_ILN_110
912			\|a GBV_ILN_138
912			\|a GBV_ILN_150
912			\|a GBV_ILN_151
912			\|a GBV_ILN_152
912			\|a GBV_ILN_161
912			\|a GBV_ILN_170
912			\|a GBV_ILN_171
912			\|a GBV_ILN_187
912			\|a GBV_ILN_213
912			\|a GBV_ILN_224
912			\|a GBV_ILN_230
912			\|a GBV_ILN_250
912			\|a GBV_ILN_281
912			\|a GBV_ILN_285
912			\|a GBV_ILN_293
912			\|a GBV_ILN_370
912			\|a GBV_ILN_602
912			\|a GBV_ILN_636
912			\|a GBV_ILN_702
912			\|a GBV_ILN_2001
912			\|a GBV_ILN_2003
912			\|a GBV_ILN_2004
912			\|a GBV_ILN_2005
912			\|a GBV_ILN_2006
912			\|a GBV_ILN_2007
912			\|a GBV_ILN_2008
912			\|a GBV_ILN_2009
912			\|a GBV_ILN_2010
912			\|a GBV_ILN_2011
912			\|a GBV_ILN_2014
912			\|a GBV_ILN_2015
912			\|a GBV_ILN_2020
912			\|a GBV_ILN_2021
912			\|a GBV_ILN_2025
912			\|a GBV_ILN_2026
912			\|a GBV_ILN_2027
912			\|a GBV_ILN_2031
912			\|a GBV_ILN_2034
912			\|a GBV_ILN_2037
912			\|a GBV_ILN_2038
912			\|a GBV_ILN_2039
912			\|a GBV_ILN_2044
912			\|a GBV_ILN_2048
912			\|a GBV_ILN_2049
912			\|a GBV_ILN_2050
912			\|a GBV_ILN_2055
912			\|a GBV_ILN_2056
912			\|a GBV_ILN_2057
912			\|a GBV_ILN_2059
912			\|a GBV_ILN_2061
912			\|a GBV_ILN_2064
912			\|a GBV_ILN_2065
912			\|a GBV_ILN_2068
912			\|a GBV_ILN_2088
912			\|a GBV_ILN_2093
912			\|a GBV_ILN_2106
912			\|a GBV_ILN_2107
912			\|a GBV_ILN_2108
912			\|a GBV_ILN_2110
912			\|a GBV_ILN_2111
912			\|a GBV_ILN_2112
912			\|a GBV_ILN_2113
912			\|a GBV_ILN_2118
912			\|a GBV_ILN_2122
912			\|a GBV_ILN_2129
912			\|a GBV_ILN_2143
912			\|a GBV_ILN_2144
912			\|a GBV_ILN_2147
912			\|a GBV_ILN_2148
912			\|a GBV_ILN_2152
912			\|a GBV_ILN_2153
912			\|a GBV_ILN_2190
912			\|a GBV_ILN_2232
912			\|a GBV_ILN_2336
912			\|a GBV_ILN_2446
912			\|a GBV_ILN_2470
912			\|a GBV_ILN_2472
912			\|a GBV_ILN_2507
912			\|a GBV_ILN_2522
912			\|a GBV_ILN_4035
912			\|a GBV_ILN_4037
912			\|a GBV_ILN_4046
912			\|a GBV_ILN_4112
912			\|a GBV_ILN_4125
912			\|a GBV_ILN_4126
912			\|a GBV_ILN_4242
912			\|a GBV_ILN_4246
912			\|a GBV_ILN_4249
912			\|a GBV_ILN_4251
912			\|a GBV_ILN_4305
912			\|a GBV_ILN_4306
912			\|a GBV_ILN_4307
912			\|a GBV_ILN_4313
912			\|a GBV_ILN_4322
912			\|a GBV_ILN_4323
912			\|a GBV_ILN_4324
912			\|a GBV_ILN_4325
912			\|a GBV_ILN_4326
912			\|a GBV_ILN_4328
912			\|a GBV_ILN_4333
912			\|a GBV_ILN_4334
912			\|a GBV_ILN_4335
912			\|a GBV_ILN_4336
912			\|a GBV_ILN_4338
912			\|a GBV_ILN_4393
912			\|a GBV_ILN_4700
951			\|a AR
952			\|d 33 \|j 2023 \|e 5 \|b 03 \|c 07 \|h 1072-1077

Indexfelder

author_variant	s s é d ssé sséd a s as g s a gs gsa m r p c mrp mrpc p s b m psb psbm f p s m m d fpsmm fpsmmd o a j b d oajb oajbd r a c g rac racg
matchkey_str	article:1981528X:2023----::hleinlssnatfnaatvtocaoeadalsutr
hierarchy_sort_str	2023
publishDate	2023
allfields	10.1007/s43450-023-00418-2 doi (DE-627)SPR052933156 (SPR)s43450-023-00418-2-e DE-627 ger DE-627 rakwb eng da Silva Santos, Éverton verfasserin (orcid)0000-0003-2400-2898 aut UHPLC-ESI Analysis and Antifungal Activity of Cladodes and Callus Cultures of Cereus hildmannianus 2023 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s) under exclusive licence to Sociedade Brasileira de Farmacognosia 2023 Plant biotechnology has a great potential in providing bioactive compounds with pharmacological applications. Bioactive compounds have been highlighted as an alternative antifungal treatment option due to the limited number of antifungals currently available. The aim of this study was to evaluate the antifungal activity and chemical profile of Cereus hildmannianus K.Schum., Cactaceae, cladodes and callus cultures. The cladodes of wild plants were collected, and the conventional callus was cultivated using agar and Murashige and Skoog medium, while the alternative culture was cultivated using a cotton support covered with filter paper and the same medium (incubated at 32 °C with 16-h photoperiod). Ethanolic extracts were prepared by Soxhlet and chemically characterized by ultra-high-performance liquid chromatography coupled with electrospray ionization quadrupole time-of-flight tandem mass spectrometry (UHPLC-ESI-QToF-MS/MS). The antifungal activity was evaluated against Candida glabrata, C. parapsilosis, C. tropicalis, and C. albicans by broth microdilution. The UHPLC-ESI-QToF-MS/MS method enabled the putative identification of fifteen proto-alkaloids and alkaloids, with pellotine and mescaline described for the first time in this species. The antifungal activity of each extract was species-dependent; extracts from both the conventional and alternative callus cultures were prominent against C. glabrata (minimal inhibitory concentration of 312 µg/ml), but there was also potential inhibitory activity against other Candida species. Graphical Abstract Candidiasis (dpeaa)DE-He213 Cactaceae (dpeaa)DE-He213 Cactus (dpeaa)DE-He213 Phenethylamines (dpeaa)DE-He213 Tetrahydroisoquinoline (dpeaa)DE-He213 Tissue culture (dpeaa)DE-He213 Savam, Aline (orcid)0000-0003-2616-8816 aut Arita, Glaucia Sayuri (orcid)0000-0002-0334-7566 aut Cabral, Márcia Regina Pereira (orcid)0000-0002-4093-8808 aut Bonfim-Mendonça, Patrícia Souza (orcid)0000-0002-0116-2189 aut de Fatima Pires Silva Machado, Maria (orcid)0000-0001-7815-4606 aut de Oliveira, Arildo José Braz (orcid)0000-0001-8737-0546 aut Gonçalves, Regina Aparecida Correia (orcid)0000-0003-4070-1269 aut Enthalten in Revista Brasileira de farmacognosia Critiba : Soc. Brasileira de Farmacognosia, 1986 33(2023), 5 vom: 03. Juli, Seite 1072-1077 (DE-627)730275531 (DE-600)2690840-2 1981-528X nnns volume:33 year:2023 number:5 day:03 month:07 pages:1072-1077 https://dx.doi.org/10.1007/s43450-023-00418-2 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 33 2023 5 03 07 1072-1077
spelling	10.1007/s43450-023-00418-2 doi (DE-627)SPR052933156 (SPR)s43450-023-00418-2-e DE-627 ger DE-627 rakwb eng da Silva Santos, Éverton verfasserin (orcid)0000-0003-2400-2898 aut UHPLC-ESI Analysis and Antifungal Activity of Cladodes and Callus Cultures of Cereus hildmannianus 2023 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s) under exclusive licence to Sociedade Brasileira de Farmacognosia 2023 Plant biotechnology has a great potential in providing bioactive compounds with pharmacological applications. Bioactive compounds have been highlighted as an alternative antifungal treatment option due to the limited number of antifungals currently available. The aim of this study was to evaluate the antifungal activity and chemical profile of Cereus hildmannianus K.Schum., Cactaceae, cladodes and callus cultures. The cladodes of wild plants were collected, and the conventional callus was cultivated using agar and Murashige and Skoog medium, while the alternative culture was cultivated using a cotton support covered with filter paper and the same medium (incubated at 32 °C with 16-h photoperiod). Ethanolic extracts were prepared by Soxhlet and chemically characterized by ultra-high-performance liquid chromatography coupled with electrospray ionization quadrupole time-of-flight tandem mass spectrometry (UHPLC-ESI-QToF-MS/MS). The antifungal activity was evaluated against Candida glabrata, C. parapsilosis, C. tropicalis, and C. albicans by broth microdilution. The UHPLC-ESI-QToF-MS/MS method enabled the putative identification of fifteen proto-alkaloids and alkaloids, with pellotine and mescaline described for the first time in this species. The antifungal activity of each extract was species-dependent; extracts from both the conventional and alternative callus cultures were prominent against C. glabrata (minimal inhibitory concentration of 312 µg/ml), but there was also potential inhibitory activity against other Candida species. Graphical Abstract Candidiasis (dpeaa)DE-He213 Cactaceae (dpeaa)DE-He213 Cactus (dpeaa)DE-He213 Phenethylamines (dpeaa)DE-He213 Tetrahydroisoquinoline (dpeaa)DE-He213 Tissue culture (dpeaa)DE-He213 Savam, Aline (orcid)0000-0003-2616-8816 aut Arita, Glaucia Sayuri (orcid)0000-0002-0334-7566 aut Cabral, Márcia Regina Pereira (orcid)0000-0002-4093-8808 aut Bonfim-Mendonça, Patrícia Souza (orcid)0000-0002-0116-2189 aut de Fatima Pires Silva Machado, Maria (orcid)0000-0001-7815-4606 aut de Oliveira, Arildo José Braz (orcid)0000-0001-8737-0546 aut Gonçalves, Regina Aparecida Correia (orcid)0000-0003-4070-1269 aut Enthalten in Revista Brasileira de farmacognosia Critiba : Soc. Brasileira de Farmacognosia, 1986 33(2023), 5 vom: 03. Juli, Seite 1072-1077 (DE-627)730275531 (DE-600)2690840-2 1981-528X nnns volume:33 year:2023 number:5 day:03 month:07 pages:1072-1077 https://dx.doi.org/10.1007/s43450-023-00418-2 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 33 2023 5 03 07 1072-1077
allfields_unstemmed	10.1007/s43450-023-00418-2 doi (DE-627)SPR052933156 (SPR)s43450-023-00418-2-e DE-627 ger DE-627 rakwb eng da Silva Santos, Éverton verfasserin (orcid)0000-0003-2400-2898 aut UHPLC-ESI Analysis and Antifungal Activity of Cladodes and Callus Cultures of Cereus hildmannianus 2023 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s) under exclusive licence to Sociedade Brasileira de Farmacognosia 2023 Plant biotechnology has a great potential in providing bioactive compounds with pharmacological applications. Bioactive compounds have been highlighted as an alternative antifungal treatment option due to the limited number of antifungals currently available. The aim of this study was to evaluate the antifungal activity and chemical profile of Cereus hildmannianus K.Schum., Cactaceae, cladodes and callus cultures. The cladodes of wild plants were collected, and the conventional callus was cultivated using agar and Murashige and Skoog medium, while the alternative culture was cultivated using a cotton support covered with filter paper and the same medium (incubated at 32 °C with 16-h photoperiod). Ethanolic extracts were prepared by Soxhlet and chemically characterized by ultra-high-performance liquid chromatography coupled with electrospray ionization quadrupole time-of-flight tandem mass spectrometry (UHPLC-ESI-QToF-MS/MS). The antifungal activity was evaluated against Candida glabrata, C. parapsilosis, C. tropicalis, and C. albicans by broth microdilution. The UHPLC-ESI-QToF-MS/MS method enabled the putative identification of fifteen proto-alkaloids and alkaloids, with pellotine and mescaline described for the first time in this species. The antifungal activity of each extract was species-dependent; extracts from both the conventional and alternative callus cultures were prominent against C. glabrata (minimal inhibitory concentration of 312 µg/ml), but there was also potential inhibitory activity against other Candida species. Graphical Abstract Candidiasis (dpeaa)DE-He213 Cactaceae (dpeaa)DE-He213 Cactus (dpeaa)DE-He213 Phenethylamines (dpeaa)DE-He213 Tetrahydroisoquinoline (dpeaa)DE-He213 Tissue culture (dpeaa)DE-He213 Savam, Aline (orcid)0000-0003-2616-8816 aut Arita, Glaucia Sayuri (orcid)0000-0002-0334-7566 aut Cabral, Márcia Regina Pereira (orcid)0000-0002-4093-8808 aut Bonfim-Mendonça, Patrícia Souza (orcid)0000-0002-0116-2189 aut de Fatima Pires Silva Machado, Maria (orcid)0000-0001-7815-4606 aut de Oliveira, Arildo José Braz (orcid)0000-0001-8737-0546 aut Gonçalves, Regina Aparecida Correia (orcid)0000-0003-4070-1269 aut Enthalten in Revista Brasileira de farmacognosia Critiba : Soc. Brasileira de Farmacognosia, 1986 33(2023), 5 vom: 03. Juli, Seite 1072-1077 (DE-627)730275531 (DE-600)2690840-2 1981-528X nnns volume:33 year:2023 number:5 day:03 month:07 pages:1072-1077 https://dx.doi.org/10.1007/s43450-023-00418-2 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 33 2023 5 03 07 1072-1077
allfieldsGer	10.1007/s43450-023-00418-2 doi (DE-627)SPR052933156 (SPR)s43450-023-00418-2-e DE-627 ger DE-627 rakwb eng da Silva Santos, Éverton verfasserin (orcid)0000-0003-2400-2898 aut UHPLC-ESI Analysis and Antifungal Activity of Cladodes and Callus Cultures of Cereus hildmannianus 2023 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s) under exclusive licence to Sociedade Brasileira de Farmacognosia 2023 Plant biotechnology has a great potential in providing bioactive compounds with pharmacological applications. Bioactive compounds have been highlighted as an alternative antifungal treatment option due to the limited number of antifungals currently available. The aim of this study was to evaluate the antifungal activity and chemical profile of Cereus hildmannianus K.Schum., Cactaceae, cladodes and callus cultures. The cladodes of wild plants were collected, and the conventional callus was cultivated using agar and Murashige and Skoog medium, while the alternative culture was cultivated using a cotton support covered with filter paper and the same medium (incubated at 32 °C with 16-h photoperiod). Ethanolic extracts were prepared by Soxhlet and chemically characterized by ultra-high-performance liquid chromatography coupled with electrospray ionization quadrupole time-of-flight tandem mass spectrometry (UHPLC-ESI-QToF-MS/MS). The antifungal activity was evaluated against Candida glabrata, C. parapsilosis, C. tropicalis, and C. albicans by broth microdilution. The UHPLC-ESI-QToF-MS/MS method enabled the putative identification of fifteen proto-alkaloids and alkaloids, with pellotine and mescaline described for the first time in this species. The antifungal activity of each extract was species-dependent; extracts from both the conventional and alternative callus cultures were prominent against C. glabrata (minimal inhibitory concentration of 312 µg/ml), but there was also potential inhibitory activity against other Candida species. Graphical Abstract Candidiasis (dpeaa)DE-He213 Cactaceae (dpeaa)DE-He213 Cactus (dpeaa)DE-He213 Phenethylamines (dpeaa)DE-He213 Tetrahydroisoquinoline (dpeaa)DE-He213 Tissue culture (dpeaa)DE-He213 Savam, Aline (orcid)0000-0003-2616-8816 aut Arita, Glaucia Sayuri (orcid)0000-0002-0334-7566 aut Cabral, Márcia Regina Pereira (orcid)0000-0002-4093-8808 aut Bonfim-Mendonça, Patrícia Souza (orcid)0000-0002-0116-2189 aut de Fatima Pires Silva Machado, Maria (orcid)0000-0001-7815-4606 aut de Oliveira, Arildo José Braz (orcid)0000-0001-8737-0546 aut Gonçalves, Regina Aparecida Correia (orcid)0000-0003-4070-1269 aut Enthalten in Revista Brasileira de farmacognosia Critiba : Soc. Brasileira de Farmacognosia, 1986 33(2023), 5 vom: 03. Juli, Seite 1072-1077 (DE-627)730275531 (DE-600)2690840-2 1981-528X nnns volume:33 year:2023 number:5 day:03 month:07 pages:1072-1077 https://dx.doi.org/10.1007/s43450-023-00418-2 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 33 2023 5 03 07 1072-1077
allfieldsSound	10.1007/s43450-023-00418-2 doi (DE-627)SPR052933156 (SPR)s43450-023-00418-2-e DE-627 ger DE-627 rakwb eng da Silva Santos, Éverton verfasserin (orcid)0000-0003-2400-2898 aut UHPLC-ESI Analysis and Antifungal Activity of Cladodes and Callus Cultures of Cereus hildmannianus 2023 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s) under exclusive licence to Sociedade Brasileira de Farmacognosia 2023 Plant biotechnology has a great potential in providing bioactive compounds with pharmacological applications. Bioactive compounds have been highlighted as an alternative antifungal treatment option due to the limited number of antifungals currently available. The aim of this study was to evaluate the antifungal activity and chemical profile of Cereus hildmannianus K.Schum., Cactaceae, cladodes and callus cultures. The cladodes of wild plants were collected, and the conventional callus was cultivated using agar and Murashige and Skoog medium, while the alternative culture was cultivated using a cotton support covered with filter paper and the same medium (incubated at 32 °C with 16-h photoperiod). Ethanolic extracts were prepared by Soxhlet and chemically characterized by ultra-high-performance liquid chromatography coupled with electrospray ionization quadrupole time-of-flight tandem mass spectrometry (UHPLC-ESI-QToF-MS/MS). The antifungal activity was evaluated against Candida glabrata, C. parapsilosis, C. tropicalis, and C. albicans by broth microdilution. The UHPLC-ESI-QToF-MS/MS method enabled the putative identification of fifteen proto-alkaloids and alkaloids, with pellotine and mescaline described for the first time in this species. The antifungal activity of each extract was species-dependent; extracts from both the conventional and alternative callus cultures were prominent against C. glabrata (minimal inhibitory concentration of 312 µg/ml), but there was also potential inhibitory activity against other Candida species. Graphical Abstract Candidiasis (dpeaa)DE-He213 Cactaceae (dpeaa)DE-He213 Cactus (dpeaa)DE-He213 Phenethylamines (dpeaa)DE-He213 Tetrahydroisoquinoline (dpeaa)DE-He213 Tissue culture (dpeaa)DE-He213 Savam, Aline (orcid)0000-0003-2616-8816 aut Arita, Glaucia Sayuri (orcid)0000-0002-0334-7566 aut Cabral, Márcia Regina Pereira (orcid)0000-0002-4093-8808 aut Bonfim-Mendonça, Patrícia Souza (orcid)0000-0002-0116-2189 aut de Fatima Pires Silva Machado, Maria (orcid)0000-0001-7815-4606 aut de Oliveira, Arildo José Braz (orcid)0000-0001-8737-0546 aut Gonçalves, Regina Aparecida Correia (orcid)0000-0003-4070-1269 aut Enthalten in Revista Brasileira de farmacognosia Critiba : Soc. Brasileira de Farmacognosia, 1986 33(2023), 5 vom: 03. Juli, Seite 1072-1077 (DE-627)730275531 (DE-600)2690840-2 1981-528X nnns volume:33 year:2023 number:5 day:03 month:07 pages:1072-1077 https://dx.doi.org/10.1007/s43450-023-00418-2 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 33 2023 5 03 07 1072-1077
language	English
source	Enthalten in Revista Brasileira de farmacognosia 33(2023), 5 vom: 03. Juli, Seite 1072-1077 volume:33 year:2023 number:5 day:03 month:07 pages:1072-1077
sourceStr	Enthalten in Revista Brasileira de farmacognosia 33(2023), 5 vom: 03. Juli, Seite 1072-1077 volume:33 year:2023 number:5 day:03 month:07 pages:1072-1077
format_phy_str_mv	Article
institution	findex.gbv.de
topic_facet	Candidiasis Cactaceae Cactus Phenethylamines Tetrahydroisoquinoline Tissue culture
isfreeaccess_bool	false
container_title	Revista Brasileira de farmacognosia
authorswithroles_txt_mv	da Silva Santos, Éverton @@aut@@ Savam, Aline @@aut@@ Arita, Glaucia Sayuri @@aut@@ Cabral, Márcia Regina Pereira @@aut@@ Bonfim-Mendonça, Patrícia Souza @@aut@@ de Fatima Pires Silva Machado, Maria @@aut@@ de Oliveira, Arildo José Braz @@aut@@ Gonçalves, Regina Aparecida Correia @@aut@@
publishDateDaySort_date	2023-07-03T00:00:00Z
hierarchy_top_id	730275531
id	SPR052933156
language_de	englisch
fullrecord	<?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>01000naa a22002652 4500</leader><controlfield tag="001">SPR052933156</controlfield><controlfield tag="003">DE-627</controlfield><controlfield tag="005">20230831064742.0</controlfield><controlfield tag="007">cr uuu---uuuuu</controlfield><controlfield tag="008">230831s2023 xx \|\|\|\|\|o 00\| \|\|eng c</controlfield><datafield tag="024" ind1="7" ind2=" "><subfield code="a">10.1007/s43450-023-00418-2</subfield><subfield code="2">doi</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-627)SPR052933156</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(SPR)s43450-023-00418-2-e</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-627</subfield><subfield code="b">ger</subfield><subfield code="c">DE-627</subfield><subfield code="e">rakwb</subfield></datafield><datafield tag="041" ind1=" " ind2=" "><subfield code="a">eng</subfield></datafield><datafield tag="100" ind1="1" ind2=" "><subfield code="a">da Silva Santos, Éverton</subfield><subfield code="e">verfasserin</subfield><subfield code="0">(orcid)0000-0003-2400-2898</subfield><subfield code="4">aut</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">UHPLC-ESI Analysis and Antifungal Activity of Cladodes and Callus Cultures of Cereus hildmannianus</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="c">2023</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="a">Text</subfield><subfield code="b">txt</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="a">Computermedien</subfield><subfield code="b">c</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="a">Online-Ressource</subfield><subfield code="b">cr</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="500" ind1=" " ind2=" "><subfield code="a">© The Author(s) under exclusive licence to Sociedade Brasileira de Farmacognosia 2023</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a">Plant biotechnology has a great potential in providing bioactive compounds with pharmacological applications. Bioactive compounds have been highlighted as an alternative antifungal treatment option due to the limited number of antifungals currently available. The aim of this study was to evaluate the antifungal activity and chemical profile of Cereus hildmannianus K.Schum., Cactaceae, cladodes and callus cultures. The cladodes of wild plants were collected, and the conventional callus was cultivated using agar and Murashige and Skoog medium, while the alternative culture was cultivated using a cotton support covered with filter paper and the same medium (incubated at 32 °C with 16-h photoperiod). Ethanolic extracts were prepared by Soxhlet and chemically characterized by ultra-high-performance liquid chromatography coupled with electrospray ionization quadrupole time-of-flight tandem mass spectrometry (UHPLC-ESI-QToF-MS/MS). The antifungal activity was evaluated against Candida glabrata, C. parapsilosis, C. tropicalis, and C. albicans by broth microdilution. The UHPLC-ESI-QToF-MS/MS method enabled the putative identification of fifteen proto-alkaloids and alkaloids, with pellotine and mescaline described for the first time in this species. The antifungal activity of each extract was species-dependent; extracts from both the conventional and alternative callus cultures were prominent against C. glabrata (minimal inhibitory concentration of 312 µg/ml), but there was also potential inhibitory activity against other Candida species. Graphical Abstract</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Candidiasis</subfield><subfield code="7">(dpeaa)DE-He213</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Cactaceae</subfield><subfield code="7">(dpeaa)DE-He213</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Cactus</subfield><subfield code="7">(dpeaa)DE-He213</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Phenethylamines</subfield><subfield code="7">(dpeaa)DE-He213</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Tetrahydroisoquinoline</subfield><subfield code="7">(dpeaa)DE-He213</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Tissue culture</subfield><subfield code="7">(dpeaa)DE-He213</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Savam, Aline</subfield><subfield code="0">(orcid)0000-0003-2616-8816</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Arita, Glaucia Sayuri</subfield><subfield code="0">(orcid)0000-0002-0334-7566</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Cabral, Márcia Regina Pereira</subfield><subfield code="0">(orcid)0000-0002-4093-8808</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Bonfim-Mendonça, Patrícia Souza</subfield><subfield code="0">(orcid)0000-0002-0116-2189</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">de Fatima Pires Silva Machado, Maria</subfield><subfield code="0">(orcid)0000-0001-7815-4606</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">de Oliveira, Arildo José Braz</subfield><subfield code="0">(orcid)0000-0001-8737-0546</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Gonçalves, Regina Aparecida Correia</subfield><subfield code="0">(orcid)0000-0003-4070-1269</subfield><subfield code="4">aut</subfield></datafield><datafield tag="773" ind1="0" ind2="8"><subfield code="i">Enthalten in</subfield><subfield code="t">Revista Brasileira de farmacognosia</subfield><subfield code="d">Critiba : Soc. Brasileira de Farmacognosia, 1986</subfield><subfield code="g">33(2023), 5 vom: 03. Juli, Seite 1072-1077</subfield><subfield code="w">(DE-627)730275531</subfield><subfield code="w">(DE-600)2690840-2</subfield><subfield code="x">1981-528X</subfield><subfield code="7">nnns</subfield></datafield><datafield tag="773" ind1="1" ind2="8"><subfield code="g">volume:33</subfield><subfield code="g">year:2023</subfield><subfield code="g">number:5</subfield><subfield code="g">day:03</subfield><subfield code="g">month:07</subfield><subfield code="g">pages:1072-1077</subfield></datafield><datafield tag="856" ind1="4" ind2="0"><subfield code="u">https://dx.doi.org/10.1007/s43450-023-00418-2</subfield><subfield code="z">lizenzpflichtig</subfield><subfield code="3">Volltext</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_USEFLAG_A</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">SYSFLAG_A</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_SPRINGER</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_11</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_20</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_22</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_23</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_24</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_31</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_32</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_39</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_40</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_60</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_62</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_63</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_65</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_69</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_70</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_73</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_74</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_90</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_95</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_100</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_101</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_105</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_110</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_138</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_150</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_151</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_152</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_161</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_170</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_171</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_187</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_213</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_224</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_230</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_250</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_281</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_285</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_293</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_370</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_602</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_636</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_702</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2001</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2003</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2004</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2005</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2006</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2007</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2008</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2009</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2010</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2011</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2014</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2015</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2020</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2021</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2025</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2026</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2027</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2031</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2034</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2037</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2038</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2039</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2044</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2048</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2049</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2050</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2055</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2056</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2057</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2059</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2061</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2064</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2065</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2068</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2088</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2093</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2106</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2107</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2108</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2110</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2111</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2112</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2113</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2118</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2122</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2129</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2143</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2144</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2147</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2148</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2152</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2153</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2190</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2232</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2336</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2446</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2470</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2472</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2507</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2522</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4035</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4037</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4046</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4112</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4125</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4126</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4242</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4246</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4249</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4251</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4305</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4306</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4307</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4313</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4322</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4323</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4324</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4325</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4326</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4328</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4333</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4334</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4335</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4336</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4338</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4393</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4700</subfield></datafield><datafield tag="951" ind1=" " ind2=" "><subfield code="a">AR</subfield></datafield><datafield tag="952" ind1=" " ind2=" "><subfield code="d">33</subfield><subfield code="j">2023</subfield><subfield code="e">5</subfield><subfield code="b">03</subfield><subfield code="c">07</subfield><subfield code="h">1072-1077</subfield></datafield></record></collection>
author	da Silva Santos, Éverton
spellingShingle	da Silva Santos, Éverton misc Candidiasis misc Cactaceae misc Cactus misc Phenethylamines misc Tetrahydroisoquinoline misc Tissue culture UHPLC-ESI Analysis and Antifungal Activity of Cladodes and Callus Cultures of Cereus hildmannianus
authorStr	da Silva Santos, Éverton
ppnlink_with_tag_str_mv	@@773@@(DE-627)730275531
format	electronic Article
delete_txt_mv	keep
author_role	aut aut aut aut aut aut aut aut
collection	springer
remote_str	true
illustrated	Not Illustrated
issn	1981-528X
topic_title	UHPLC-ESI Analysis and Antifungal Activity of Cladodes and Callus Cultures of Cereus hildmannianus Candidiasis (dpeaa)DE-He213 Cactaceae (dpeaa)DE-He213 Cactus (dpeaa)DE-He213 Phenethylamines (dpeaa)DE-He213 Tetrahydroisoquinoline (dpeaa)DE-He213 Tissue culture (dpeaa)DE-He213
topic	misc Candidiasis misc Cactaceae misc Cactus misc Phenethylamines misc Tetrahydroisoquinoline misc Tissue culture
topic_unstemmed	misc Candidiasis misc Cactaceae misc Cactus misc Phenethylamines misc Tetrahydroisoquinoline misc Tissue culture
topic_browse	misc Candidiasis misc Cactaceae misc Cactus misc Phenethylamines misc Tetrahydroisoquinoline misc Tissue culture
format_facet	Elektronische Aufsätze Aufsätze Elektronische Ressource
format_main_str_mv	Text Zeitschrift/Artikel
carriertype_str_mv	cr
hierarchy_parent_title	Revista Brasileira de farmacognosia
hierarchy_parent_id	730275531
hierarchy_top_title	Revista Brasileira de farmacognosia
isfreeaccess_txt	false
familylinks_str_mv	(DE-627)730275531 (DE-600)2690840-2
title	UHPLC-ESI Analysis and Antifungal Activity of Cladodes and Callus Cultures of Cereus hildmannianus
ctrlnum	(DE-627)SPR052933156 (SPR)s43450-023-00418-2-e
title_full	UHPLC-ESI Analysis and Antifungal Activity of Cladodes and Callus Cultures of Cereus hildmannianus
author_sort	da Silva Santos, Éverton
journal	Revista Brasileira de farmacognosia
journalStr	Revista Brasileira de farmacognosia
lang_code	eng
isOA_bool	false
recordtype	marc
publishDateSort	2023
contenttype_str_mv	txt
container_start_page	1072
author_browse	da Silva Santos, Éverton Savam, Aline Arita, Glaucia Sayuri Cabral, Márcia Regina Pereira Bonfim-Mendonça, Patrícia Souza de Fatima Pires Silva Machado, Maria de Oliveira, Arildo José Braz Gonçalves, Regina Aparecida Correia
container_volume	33
format_se	Elektronische Aufsätze
author-letter	da Silva Santos, Éverton
doi_str_mv	10.1007/s43450-023-00418-2
normlink	(ORCID)0000-0003-2400-2898 (ORCID)0000-0003-2616-8816 (ORCID)0000-0002-0334-7566 (ORCID)0000-0002-4093-8808 (ORCID)0000-0002-0116-2189 (ORCID)0000-0001-7815-4606 (ORCID)0000-0001-8737-0546 (ORCID)0000-0003-4070-1269
normlink_prefix_str_mv	(orcid)0000-0003-2400-2898 (orcid)0000-0003-2616-8816 (orcid)0000-0002-0334-7566 (orcid)0000-0002-4093-8808 (orcid)0000-0002-0116-2189 (orcid)0000-0001-7815-4606 (orcid)0000-0001-8737-0546 (orcid)0000-0003-4070-1269
title_sort	uhplc-esi analysis and antifungal activity of cladodes and callus cultures of cereus hildmannianus
title_auth	UHPLC-ESI Analysis and Antifungal Activity of Cladodes and Callus Cultures of Cereus hildmannianus
abstract	Plant biotechnology has a great potential in providing bioactive compounds with pharmacological applications. Bioactive compounds have been highlighted as an alternative antifungal treatment option due to the limited number of antifungals currently available. The aim of this study was to evaluate the antifungal activity and chemical profile of Cereus hildmannianus K.Schum., Cactaceae, cladodes and callus cultures. The cladodes of wild plants were collected, and the conventional callus was cultivated using agar and Murashige and Skoog medium, while the alternative culture was cultivated using a cotton support covered with filter paper and the same medium (incubated at 32 °C with 16-h photoperiod). Ethanolic extracts were prepared by Soxhlet and chemically characterized by ultra-high-performance liquid chromatography coupled with electrospray ionization quadrupole time-of-flight tandem mass spectrometry (UHPLC-ESI-QToF-MS/MS). The antifungal activity was evaluated against Candida glabrata, C. parapsilosis, C. tropicalis, and C. albicans by broth microdilution. The UHPLC-ESI-QToF-MS/MS method enabled the putative identification of fifteen proto-alkaloids and alkaloids, with pellotine and mescaline described for the first time in this species. The antifungal activity of each extract was species-dependent; extracts from both the conventional and alternative callus cultures were prominent against C. glabrata (minimal inhibitory concentration of 312 µg/ml), but there was also potential inhibitory activity against other Candida species. Graphical Abstract © The Author(s) under exclusive licence to Sociedade Brasileira de Farmacognosia 2023
abstractGer	Plant biotechnology has a great potential in providing bioactive compounds with pharmacological applications. Bioactive compounds have been highlighted as an alternative antifungal treatment option due to the limited number of antifungals currently available. The aim of this study was to evaluate the antifungal activity and chemical profile of Cereus hildmannianus K.Schum., Cactaceae, cladodes and callus cultures. The cladodes of wild plants were collected, and the conventional callus was cultivated using agar and Murashige and Skoog medium, while the alternative culture was cultivated using a cotton support covered with filter paper and the same medium (incubated at 32 °C with 16-h photoperiod). Ethanolic extracts were prepared by Soxhlet and chemically characterized by ultra-high-performance liquid chromatography coupled with electrospray ionization quadrupole time-of-flight tandem mass spectrometry (UHPLC-ESI-QToF-MS/MS). The antifungal activity was evaluated against Candida glabrata, C. parapsilosis, C. tropicalis, and C. albicans by broth microdilution. The UHPLC-ESI-QToF-MS/MS method enabled the putative identification of fifteen proto-alkaloids and alkaloids, with pellotine and mescaline described for the first time in this species. The antifungal activity of each extract was species-dependent; extracts from both the conventional and alternative callus cultures were prominent against C. glabrata (minimal inhibitory concentration of 312 µg/ml), but there was also potential inhibitory activity against other Candida species. Graphical Abstract © The Author(s) under exclusive licence to Sociedade Brasileira de Farmacognosia 2023
abstract_unstemmed	Plant biotechnology has a great potential in providing bioactive compounds with pharmacological applications. Bioactive compounds have been highlighted as an alternative antifungal treatment option due to the limited number of antifungals currently available. The aim of this study was to evaluate the antifungal activity and chemical profile of Cereus hildmannianus K.Schum., Cactaceae, cladodes and callus cultures. The cladodes of wild plants were collected, and the conventional callus was cultivated using agar and Murashige and Skoog medium, while the alternative culture was cultivated using a cotton support covered with filter paper and the same medium (incubated at 32 °C with 16-h photoperiod). Ethanolic extracts were prepared by Soxhlet and chemically characterized by ultra-high-performance liquid chromatography coupled with electrospray ionization quadrupole time-of-flight tandem mass spectrometry (UHPLC-ESI-QToF-MS/MS). The antifungal activity was evaluated against Candida glabrata, C. parapsilosis, C. tropicalis, and C. albicans by broth microdilution. The UHPLC-ESI-QToF-MS/MS method enabled the putative identification of fifteen proto-alkaloids and alkaloids, with pellotine and mescaline described for the first time in this species. The antifungal activity of each extract was species-dependent; extracts from both the conventional and alternative callus cultures were prominent against C. glabrata (minimal inhibitory concentration of 312 µg/ml), but there was also potential inhibitory activity against other Candida species. Graphical Abstract © The Author(s) under exclusive licence to Sociedade Brasileira de Farmacognosia 2023
collection_details	GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700
container_issue	5
title_short	UHPLC-ESI Analysis and Antifungal Activity of Cladodes and Callus Cultures of Cereus hildmannianus
url	https://dx.doi.org/10.1007/s43450-023-00418-2
remote_bool	true
author2	Savam, Aline Arita, Glaucia Sayuri Cabral, Márcia Regina Pereira Bonfim-Mendonça, Patrícia Souza de Fatima Pires Silva Machado, Maria de Oliveira, Arildo José Braz Gonçalves, Regina Aparecida Correia
author2Str	Savam, Aline Arita, Glaucia Sayuri Cabral, Márcia Regina Pereira Bonfim-Mendonça, Patrícia Souza de Fatima Pires Silva Machado, Maria de Oliveira, Arildo José Braz Gonçalves, Regina Aparecida Correia
ppnlink	730275531
mediatype_str_mv	c
isOA_txt	false
hochschulschrift_bool	false
doi_str	10.1007/s43450-023-00418-2
up_date	2024-07-03T15:51:15.505Z
_version_	1803573658131628032
fullrecord_marcxml	<?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>01000naa a22002652 4500</leader><controlfield tag="001">SPR052933156</controlfield><controlfield tag="003">DE-627</controlfield><controlfield tag="005">20230831064742.0</controlfield><controlfield tag="007">cr uuu---uuuuu</controlfield><controlfield tag="008">230831s2023 xx \|\|\|\|\|o 00\| \|\|eng c</controlfield><datafield tag="024" ind1="7" ind2=" "><subfield code="a">10.1007/s43450-023-00418-2</subfield><subfield code="2">doi</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-627)SPR052933156</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(SPR)s43450-023-00418-2-e</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-627</subfield><subfield code="b">ger</subfield><subfield code="c">DE-627</subfield><subfield code="e">rakwb</subfield></datafield><datafield tag="041" ind1=" " ind2=" "><subfield code="a">eng</subfield></datafield><datafield tag="100" ind1="1" ind2=" "><subfield code="a">da Silva Santos, Éverton</subfield><subfield code="e">verfasserin</subfield><subfield code="0">(orcid)0000-0003-2400-2898</subfield><subfield code="4">aut</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">UHPLC-ESI Analysis and Antifungal Activity of Cladodes and Callus Cultures of Cereus hildmannianus</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="c">2023</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="a">Text</subfield><subfield code="b">txt</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="a">Computermedien</subfield><subfield code="b">c</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="a">Online-Ressource</subfield><subfield code="b">cr</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="500" ind1=" " ind2=" "><subfield code="a">© The Author(s) under exclusive licence to Sociedade Brasileira de Farmacognosia 2023</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a">Plant biotechnology has a great potential in providing bioactive compounds with pharmacological applications. Bioactive compounds have been highlighted as an alternative antifungal treatment option due to the limited number of antifungals currently available. The aim of this study was to evaluate the antifungal activity and chemical profile of Cereus hildmannianus K.Schum., Cactaceae, cladodes and callus cultures. The cladodes of wild plants were collected, and the conventional callus was cultivated using agar and Murashige and Skoog medium, while the alternative culture was cultivated using a cotton support covered with filter paper and the same medium (incubated at 32 °C with 16-h photoperiod). Ethanolic extracts were prepared by Soxhlet and chemically characterized by ultra-high-performance liquid chromatography coupled with electrospray ionization quadrupole time-of-flight tandem mass spectrometry (UHPLC-ESI-QToF-MS/MS). The antifungal activity was evaluated against Candida glabrata, C. parapsilosis, C. tropicalis, and C. albicans by broth microdilution. The UHPLC-ESI-QToF-MS/MS method enabled the putative identification of fifteen proto-alkaloids and alkaloids, with pellotine and mescaline described for the first time in this species. The antifungal activity of each extract was species-dependent; extracts from both the conventional and alternative callus cultures were prominent against C. glabrata (minimal inhibitory concentration of 312 µg/ml), but there was also potential inhibitory activity against other Candida species. Graphical Abstract</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Candidiasis</subfield><subfield code="7">(dpeaa)DE-He213</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Cactaceae</subfield><subfield code="7">(dpeaa)DE-He213</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Cactus</subfield><subfield code="7">(dpeaa)DE-He213</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Phenethylamines</subfield><subfield code="7">(dpeaa)DE-He213</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Tetrahydroisoquinoline</subfield><subfield code="7">(dpeaa)DE-He213</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Tissue culture</subfield><subfield code="7">(dpeaa)DE-He213</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Savam, Aline</subfield><subfield code="0">(orcid)0000-0003-2616-8816</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Arita, Glaucia Sayuri</subfield><subfield code="0">(orcid)0000-0002-0334-7566</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Cabral, Márcia Regina Pereira</subfield><subfield code="0">(orcid)0000-0002-4093-8808</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Bonfim-Mendonça, Patrícia Souza</subfield><subfield code="0">(orcid)0000-0002-0116-2189</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">de Fatima Pires Silva Machado, Maria</subfield><subfield code="0">(orcid)0000-0001-7815-4606</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">de Oliveira, Arildo José Braz</subfield><subfield code="0">(orcid)0000-0001-8737-0546</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Gonçalves, Regina Aparecida Correia</subfield><subfield code="0">(orcid)0000-0003-4070-1269</subfield><subfield code="4">aut</subfield></datafield><datafield tag="773" ind1="0" ind2="8"><subfield code="i">Enthalten in</subfield><subfield code="t">Revista Brasileira de farmacognosia</subfield><subfield code="d">Critiba : Soc. Brasileira de Farmacognosia, 1986</subfield><subfield code="g">33(2023), 5 vom: 03. Juli, Seite 1072-1077</subfield><subfield code="w">(DE-627)730275531</subfield><subfield code="w">(DE-600)2690840-2</subfield><subfield code="x">1981-528X</subfield><subfield code="7">nnns</subfield></datafield><datafield tag="773" ind1="1" ind2="8"><subfield code="g">volume:33</subfield><subfield code="g">year:2023</subfield><subfield code="g">number:5</subfield><subfield code="g">day:03</subfield><subfield code="g">month:07</subfield><subfield code="g">pages:1072-1077</subfield></datafield><datafield tag="856" ind1="4" ind2="0"><subfield code="u">https://dx.doi.org/10.1007/s43450-023-00418-2</subfield><subfield code="z">lizenzpflichtig</subfield><subfield code="3">Volltext</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_USEFLAG_A</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">SYSFLAG_A</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_SPRINGER</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_11</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_20</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_22</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_23</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_24</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_31</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_32</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_39</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_40</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_60</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_62</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_63</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_65</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_69</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_70</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_73</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_74</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_90</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_95</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_100</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_101</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_105</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_110</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_138</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_150</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_151</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_152</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_161</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_170</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_171</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_187</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_213</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_224</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_230</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_250</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_281</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_285</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_293</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_370</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_602</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_636</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_702</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2001</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2003</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2004</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2005</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2006</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2007</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2008</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2009</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2010</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2011</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2014</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2015</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2020</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2021</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2025</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2026</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2027</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2031</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2034</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2037</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2038</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2039</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2044</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2048</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2049</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2050</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2055</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2056</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2057</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2059</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2061</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2064</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2065</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2068</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2088</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2093</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2106</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2107</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2108</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2110</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2111</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2112</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2113</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2118</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2122</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2129</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2143</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2144</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2147</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2148</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2152</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2153</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2190</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2232</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2336</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2446</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2470</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2472</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2507</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2522</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4035</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4037</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4046</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4112</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4125</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4126</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4242</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4246</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4249</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4251</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4305</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4306</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4307</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4313</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4322</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4323</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4324</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4325</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4326</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4328</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4333</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4334</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4335</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4336</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4338</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4393</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4700</subfield></datafield><datafield tag="951" ind1=" " ind2=" "><subfield code="a">AR</subfield></datafield><datafield tag="952" ind1=" " ind2=" "><subfield code="d">33</subfield><subfield code="j">2023</subfield><subfield code="e">5</subfield><subfield code="b">03</subfield><subfield code="c">07</subfield><subfield code="h">1072-1077</subfield></datafield></record></collection>
score	7.4010077

Nicht das Richtige dabei?

Schreiben Sie uns!

UHPLC-ESI Analysis and Antifungal Activity of Cladodes and Callus Cultures of Cereus hildmannianus

Nicht das Richtige dabei?

Zugang & Verfügbarkeit

Vorhandene Bände

Nicht das Richtige dabei?