UHPLC-ESI Analysis and Antifungal Activity of Cladodes and Callus Cultures of Cereus hildmannianus
Plant biotechnology has a great potential in providing bioactive compounds with pharmacological applications. Bioactive compounds have been highlighted as an alternative antifungal treatment option due to the limited number of antifungals currently available. The aim of this study was to evaluate th...
Ausführliche Beschreibung
Autor*in: |
da Silva Santos, Éverton [verfasserIn] Bonfim-Mendonça, Patrícia Souza |
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Format: |
E-Artikel |
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Sprache: |
Englisch |
Erschienen: |
2023 |
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Schlagwörter: |
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Anmerkung: |
© The Author(s) under exclusive licence to Sociedade Brasileira de Farmacognosia 2023 |
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Übergeordnetes Werk: |
Enthalten in: Revista Brasileira de farmacognosia - Critiba : Soc. Brasileira de Farmacognosia, 1986, 33(2023), 5 vom: 03. Juli, Seite 1072-1077 |
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Übergeordnetes Werk: |
volume:33 ; year:2023 ; number:5 ; day:03 ; month:07 ; pages:1072-1077 |
Links: |
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DOI / URN: |
10.1007/s43450-023-00418-2 |
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Katalog-ID: |
SPR052933156 |
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520 | |a Plant biotechnology has a great potential in providing bioactive compounds with pharmacological applications. Bioactive compounds have been highlighted as an alternative antifungal treatment option due to the limited number of antifungals currently available. The aim of this study was to evaluate the antifungal activity and chemical profile of Cereus hildmannianus K.Schum., Cactaceae, cladodes and callus cultures. The cladodes of wild plants were collected, and the conventional callus was cultivated using agar and Murashige and Skoog medium, while the alternative culture was cultivated using a cotton support covered with filter paper and the same medium (incubated at 32 °C with 16-h photoperiod). Ethanolic extracts were prepared by Soxhlet and chemically characterized by ultra-high-performance liquid chromatography coupled with electrospray ionization quadrupole time-of-flight tandem mass spectrometry (UHPLC-ESI-QToF-MS/MS). The antifungal activity was evaluated against Candida glabrata, C. parapsilosis, C. tropicalis, and C. albicans by broth microdilution. The UHPLC-ESI-QToF-MS/MS method enabled the putative identification of fifteen proto-alkaloids and alkaloids, with pellotine and mescaline described for the first time in this species. The antifungal activity of each extract was species-dependent; extracts from both the conventional and alternative callus cultures were prominent against C. glabrata (minimal inhibitory concentration of 312 µg/ml), but there was also potential inhibitory activity against other Candida species. Graphical Abstract | ||
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10.1007/s43450-023-00418-2 doi (DE-627)SPR052933156 (SPR)s43450-023-00418-2-e DE-627 ger DE-627 rakwb eng da Silva Santos, Éverton verfasserin (orcid)0000-0003-2400-2898 aut UHPLC-ESI Analysis and Antifungal Activity of Cladodes and Callus Cultures of Cereus hildmannianus 2023 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s) under exclusive licence to Sociedade Brasileira de Farmacognosia 2023 Plant biotechnology has a great potential in providing bioactive compounds with pharmacological applications. Bioactive compounds have been highlighted as an alternative antifungal treatment option due to the limited number of antifungals currently available. The aim of this study was to evaluate the antifungal activity and chemical profile of Cereus hildmannianus K.Schum., Cactaceae, cladodes and callus cultures. The cladodes of wild plants were collected, and the conventional callus was cultivated using agar and Murashige and Skoog medium, while the alternative culture was cultivated using a cotton support covered with filter paper and the same medium (incubated at 32 °C with 16-h photoperiod). Ethanolic extracts were prepared by Soxhlet and chemically characterized by ultra-high-performance liquid chromatography coupled with electrospray ionization quadrupole time-of-flight tandem mass spectrometry (UHPLC-ESI-QToF-MS/MS). The antifungal activity was evaluated against Candida glabrata, C. parapsilosis, C. tropicalis, and C. albicans by broth microdilution. The UHPLC-ESI-QToF-MS/MS method enabled the putative identification of fifteen proto-alkaloids and alkaloids, with pellotine and mescaline described for the first time in this species. The antifungal activity of each extract was species-dependent; extracts from both the conventional and alternative callus cultures were prominent against C. glabrata (minimal inhibitory concentration of 312 µg/ml), but there was also potential inhibitory activity against other Candida species. Graphical Abstract Candidiasis (dpeaa)DE-He213 Cactaceae (dpeaa)DE-He213 Cactus (dpeaa)DE-He213 Phenethylamines (dpeaa)DE-He213 Tetrahydroisoquinoline (dpeaa)DE-He213 Tissue culture (dpeaa)DE-He213 Savam, Aline (orcid)0000-0003-2616-8816 aut Arita, Glaucia Sayuri (orcid)0000-0002-0334-7566 aut Cabral, Márcia Regina Pereira (orcid)0000-0002-4093-8808 aut Bonfim-Mendonça, Patrícia Souza (orcid)0000-0002-0116-2189 aut de Fatima Pires Silva Machado, Maria (orcid)0000-0001-7815-4606 aut de Oliveira, Arildo José Braz (orcid)0000-0001-8737-0546 aut Gonçalves, Regina Aparecida Correia (orcid)0000-0003-4070-1269 aut Enthalten in Revista Brasileira de farmacognosia Critiba : Soc. Brasileira de Farmacognosia, 1986 33(2023), 5 vom: 03. Juli, Seite 1072-1077 (DE-627)730275531 (DE-600)2690840-2 1981-528X nnns volume:33 year:2023 number:5 day:03 month:07 pages:1072-1077 https://dx.doi.org/10.1007/s43450-023-00418-2 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 33 2023 5 03 07 1072-1077 |
spelling |
10.1007/s43450-023-00418-2 doi (DE-627)SPR052933156 (SPR)s43450-023-00418-2-e DE-627 ger DE-627 rakwb eng da Silva Santos, Éverton verfasserin (orcid)0000-0003-2400-2898 aut UHPLC-ESI Analysis and Antifungal Activity of Cladodes and Callus Cultures of Cereus hildmannianus 2023 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s) under exclusive licence to Sociedade Brasileira de Farmacognosia 2023 Plant biotechnology has a great potential in providing bioactive compounds with pharmacological applications. Bioactive compounds have been highlighted as an alternative antifungal treatment option due to the limited number of antifungals currently available. The aim of this study was to evaluate the antifungal activity and chemical profile of Cereus hildmannianus K.Schum., Cactaceae, cladodes and callus cultures. The cladodes of wild plants were collected, and the conventional callus was cultivated using agar and Murashige and Skoog medium, while the alternative culture was cultivated using a cotton support covered with filter paper and the same medium (incubated at 32 °C with 16-h photoperiod). Ethanolic extracts were prepared by Soxhlet and chemically characterized by ultra-high-performance liquid chromatography coupled with electrospray ionization quadrupole time-of-flight tandem mass spectrometry (UHPLC-ESI-QToF-MS/MS). The antifungal activity was evaluated against Candida glabrata, C. parapsilosis, C. tropicalis, and C. albicans by broth microdilution. The UHPLC-ESI-QToF-MS/MS method enabled the putative identification of fifteen proto-alkaloids and alkaloids, with pellotine and mescaline described for the first time in this species. The antifungal activity of each extract was species-dependent; extracts from both the conventional and alternative callus cultures were prominent against C. glabrata (minimal inhibitory concentration of 312 µg/ml), but there was also potential inhibitory activity against other Candida species. Graphical Abstract Candidiasis (dpeaa)DE-He213 Cactaceae (dpeaa)DE-He213 Cactus (dpeaa)DE-He213 Phenethylamines (dpeaa)DE-He213 Tetrahydroisoquinoline (dpeaa)DE-He213 Tissue culture (dpeaa)DE-He213 Savam, Aline (orcid)0000-0003-2616-8816 aut Arita, Glaucia Sayuri (orcid)0000-0002-0334-7566 aut Cabral, Márcia Regina Pereira (orcid)0000-0002-4093-8808 aut Bonfim-Mendonça, Patrícia Souza (orcid)0000-0002-0116-2189 aut de Fatima Pires Silva Machado, Maria (orcid)0000-0001-7815-4606 aut de Oliveira, Arildo José Braz (orcid)0000-0001-8737-0546 aut Gonçalves, Regina Aparecida Correia (orcid)0000-0003-4070-1269 aut Enthalten in Revista Brasileira de farmacognosia Critiba : Soc. Brasileira de Farmacognosia, 1986 33(2023), 5 vom: 03. Juli, Seite 1072-1077 (DE-627)730275531 (DE-600)2690840-2 1981-528X nnns volume:33 year:2023 number:5 day:03 month:07 pages:1072-1077 https://dx.doi.org/10.1007/s43450-023-00418-2 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 33 2023 5 03 07 1072-1077 |
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10.1007/s43450-023-00418-2 doi (DE-627)SPR052933156 (SPR)s43450-023-00418-2-e DE-627 ger DE-627 rakwb eng da Silva Santos, Éverton verfasserin (orcid)0000-0003-2400-2898 aut UHPLC-ESI Analysis and Antifungal Activity of Cladodes and Callus Cultures of Cereus hildmannianus 2023 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s) under exclusive licence to Sociedade Brasileira de Farmacognosia 2023 Plant biotechnology has a great potential in providing bioactive compounds with pharmacological applications. Bioactive compounds have been highlighted as an alternative antifungal treatment option due to the limited number of antifungals currently available. The aim of this study was to evaluate the antifungal activity and chemical profile of Cereus hildmannianus K.Schum., Cactaceae, cladodes and callus cultures. The cladodes of wild plants were collected, and the conventional callus was cultivated using agar and Murashige and Skoog medium, while the alternative culture was cultivated using a cotton support covered with filter paper and the same medium (incubated at 32 °C with 16-h photoperiod). Ethanolic extracts were prepared by Soxhlet and chemically characterized by ultra-high-performance liquid chromatography coupled with electrospray ionization quadrupole time-of-flight tandem mass spectrometry (UHPLC-ESI-QToF-MS/MS). The antifungal activity was evaluated against Candida glabrata, C. parapsilosis, C. tropicalis, and C. albicans by broth microdilution. The UHPLC-ESI-QToF-MS/MS method enabled the putative identification of fifteen proto-alkaloids and alkaloids, with pellotine and mescaline described for the first time in this species. The antifungal activity of each extract was species-dependent; extracts from both the conventional and alternative callus cultures were prominent against C. glabrata (minimal inhibitory concentration of 312 µg/ml), but there was also potential inhibitory activity against other Candida species. Graphical Abstract Candidiasis (dpeaa)DE-He213 Cactaceae (dpeaa)DE-He213 Cactus (dpeaa)DE-He213 Phenethylamines (dpeaa)DE-He213 Tetrahydroisoquinoline (dpeaa)DE-He213 Tissue culture (dpeaa)DE-He213 Savam, Aline (orcid)0000-0003-2616-8816 aut Arita, Glaucia Sayuri (orcid)0000-0002-0334-7566 aut Cabral, Márcia Regina Pereira (orcid)0000-0002-4093-8808 aut Bonfim-Mendonça, Patrícia Souza (orcid)0000-0002-0116-2189 aut de Fatima Pires Silva Machado, Maria (orcid)0000-0001-7815-4606 aut de Oliveira, Arildo José Braz (orcid)0000-0001-8737-0546 aut Gonçalves, Regina Aparecida Correia (orcid)0000-0003-4070-1269 aut Enthalten in Revista Brasileira de farmacognosia Critiba : Soc. Brasileira de Farmacognosia, 1986 33(2023), 5 vom: 03. Juli, Seite 1072-1077 (DE-627)730275531 (DE-600)2690840-2 1981-528X nnns volume:33 year:2023 number:5 day:03 month:07 pages:1072-1077 https://dx.doi.org/10.1007/s43450-023-00418-2 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 33 2023 5 03 07 1072-1077 |
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10.1007/s43450-023-00418-2 doi (DE-627)SPR052933156 (SPR)s43450-023-00418-2-e DE-627 ger DE-627 rakwb eng da Silva Santos, Éverton verfasserin (orcid)0000-0003-2400-2898 aut UHPLC-ESI Analysis and Antifungal Activity of Cladodes and Callus Cultures of Cereus hildmannianus 2023 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s) under exclusive licence to Sociedade Brasileira de Farmacognosia 2023 Plant biotechnology has a great potential in providing bioactive compounds with pharmacological applications. Bioactive compounds have been highlighted as an alternative antifungal treatment option due to the limited number of antifungals currently available. The aim of this study was to evaluate the antifungal activity and chemical profile of Cereus hildmannianus K.Schum., Cactaceae, cladodes and callus cultures. The cladodes of wild plants were collected, and the conventional callus was cultivated using agar and Murashige and Skoog medium, while the alternative culture was cultivated using a cotton support covered with filter paper and the same medium (incubated at 32 °C with 16-h photoperiod). Ethanolic extracts were prepared by Soxhlet and chemically characterized by ultra-high-performance liquid chromatography coupled with electrospray ionization quadrupole time-of-flight tandem mass spectrometry (UHPLC-ESI-QToF-MS/MS). The antifungal activity was evaluated against Candida glabrata, C. parapsilosis, C. tropicalis, and C. albicans by broth microdilution. The UHPLC-ESI-QToF-MS/MS method enabled the putative identification of fifteen proto-alkaloids and alkaloids, with pellotine and mescaline described for the first time in this species. The antifungal activity of each extract was species-dependent; extracts from both the conventional and alternative callus cultures were prominent against C. glabrata (minimal inhibitory concentration of 312 µg/ml), but there was also potential inhibitory activity against other Candida species. Graphical Abstract Candidiasis (dpeaa)DE-He213 Cactaceae (dpeaa)DE-He213 Cactus (dpeaa)DE-He213 Phenethylamines (dpeaa)DE-He213 Tetrahydroisoquinoline (dpeaa)DE-He213 Tissue culture (dpeaa)DE-He213 Savam, Aline (orcid)0000-0003-2616-8816 aut Arita, Glaucia Sayuri (orcid)0000-0002-0334-7566 aut Cabral, Márcia Regina Pereira (orcid)0000-0002-4093-8808 aut Bonfim-Mendonça, Patrícia Souza (orcid)0000-0002-0116-2189 aut de Fatima Pires Silva Machado, Maria (orcid)0000-0001-7815-4606 aut de Oliveira, Arildo José Braz (orcid)0000-0001-8737-0546 aut Gonçalves, Regina Aparecida Correia (orcid)0000-0003-4070-1269 aut Enthalten in Revista Brasileira de farmacognosia Critiba : Soc. Brasileira de Farmacognosia, 1986 33(2023), 5 vom: 03. Juli, Seite 1072-1077 (DE-627)730275531 (DE-600)2690840-2 1981-528X nnns volume:33 year:2023 number:5 day:03 month:07 pages:1072-1077 https://dx.doi.org/10.1007/s43450-023-00418-2 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 33 2023 5 03 07 1072-1077 |
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10.1007/s43450-023-00418-2 doi (DE-627)SPR052933156 (SPR)s43450-023-00418-2-e DE-627 ger DE-627 rakwb eng da Silva Santos, Éverton verfasserin (orcid)0000-0003-2400-2898 aut UHPLC-ESI Analysis and Antifungal Activity of Cladodes and Callus Cultures of Cereus hildmannianus 2023 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s) under exclusive licence to Sociedade Brasileira de Farmacognosia 2023 Plant biotechnology has a great potential in providing bioactive compounds with pharmacological applications. Bioactive compounds have been highlighted as an alternative antifungal treatment option due to the limited number of antifungals currently available. The aim of this study was to evaluate the antifungal activity and chemical profile of Cereus hildmannianus K.Schum., Cactaceae, cladodes and callus cultures. The cladodes of wild plants were collected, and the conventional callus was cultivated using agar and Murashige and Skoog medium, while the alternative culture was cultivated using a cotton support covered with filter paper and the same medium (incubated at 32 °C with 16-h photoperiod). Ethanolic extracts were prepared by Soxhlet and chemically characterized by ultra-high-performance liquid chromatography coupled with electrospray ionization quadrupole time-of-flight tandem mass spectrometry (UHPLC-ESI-QToF-MS/MS). The antifungal activity was evaluated against Candida glabrata, C. parapsilosis, C. tropicalis, and C. albicans by broth microdilution. The UHPLC-ESI-QToF-MS/MS method enabled the putative identification of fifteen proto-alkaloids and alkaloids, with pellotine and mescaline described for the first time in this species. The antifungal activity of each extract was species-dependent; extracts from both the conventional and alternative callus cultures were prominent against C. glabrata (minimal inhibitory concentration of 312 µg/ml), but there was also potential inhibitory activity against other Candida species. Graphical Abstract Candidiasis (dpeaa)DE-He213 Cactaceae (dpeaa)DE-He213 Cactus (dpeaa)DE-He213 Phenethylamines (dpeaa)DE-He213 Tetrahydroisoquinoline (dpeaa)DE-He213 Tissue culture (dpeaa)DE-He213 Savam, Aline (orcid)0000-0003-2616-8816 aut Arita, Glaucia Sayuri (orcid)0000-0002-0334-7566 aut Cabral, Márcia Regina Pereira (orcid)0000-0002-4093-8808 aut Bonfim-Mendonça, Patrícia Souza (orcid)0000-0002-0116-2189 aut de Fatima Pires Silva Machado, Maria (orcid)0000-0001-7815-4606 aut de Oliveira, Arildo José Braz (orcid)0000-0001-8737-0546 aut Gonçalves, Regina Aparecida Correia (orcid)0000-0003-4070-1269 aut Enthalten in Revista Brasileira de farmacognosia Critiba : Soc. Brasileira de Farmacognosia, 1986 33(2023), 5 vom: 03. Juli, Seite 1072-1077 (DE-627)730275531 (DE-600)2690840-2 1981-528X nnns volume:33 year:2023 number:5 day:03 month:07 pages:1072-1077 https://dx.doi.org/10.1007/s43450-023-00418-2 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 33 2023 5 03 07 1072-1077 |
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English |
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Enthalten in Revista Brasileira de farmacognosia 33(2023), 5 vom: 03. Juli, Seite 1072-1077 volume:33 year:2023 number:5 day:03 month:07 pages:1072-1077 |
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Enthalten in Revista Brasileira de farmacognosia 33(2023), 5 vom: 03. Juli, Seite 1072-1077 volume:33 year:2023 number:5 day:03 month:07 pages:1072-1077 |
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Candidiasis Cactaceae Cactus Phenethylamines Tetrahydroisoquinoline Tissue culture |
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Revista Brasileira de farmacognosia |
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da Silva Santos, Éverton @@aut@@ Savam, Aline @@aut@@ Arita, Glaucia Sayuri @@aut@@ Cabral, Márcia Regina Pereira @@aut@@ Bonfim-Mendonça, Patrícia Souza @@aut@@ de Fatima Pires Silva Machado, Maria @@aut@@ de Oliveira, Arildo José Braz @@aut@@ Gonçalves, Regina Aparecida Correia @@aut@@ |
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Bioactive compounds have been highlighted as an alternative antifungal treatment option due to the limited number of antifungals currently available. The aim of this study was to evaluate the antifungal activity and chemical profile of Cereus hildmannianus K.Schum., Cactaceae, cladodes and callus cultures. The cladodes of wild plants were collected, and the conventional callus was cultivated using agar and Murashige and Skoog medium, while the alternative culture was cultivated using a cotton support covered with filter paper and the same medium (incubated at 32 °C with 16-h photoperiod). Ethanolic extracts were prepared by Soxhlet and chemically characterized by ultra-high-performance liquid chromatography coupled with electrospray ionization quadrupole time-of-flight tandem mass spectrometry (UHPLC-ESI-QToF-MS/MS). The antifungal activity was evaluated against Candida glabrata, C. parapsilosis, C. tropicalis, and C. albicans by broth microdilution. The UHPLC-ESI-QToF-MS/MS method enabled the putative identification of fifteen proto-alkaloids and alkaloids, with pellotine and mescaline described for the first time in this species. The antifungal activity of each extract was species-dependent; extracts from both the conventional and alternative callus cultures were prominent against C. glabrata (minimal inhibitory concentration of 312 µg/ml), but there was also potential inhibitory activity against other Candida species. 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author |
da Silva Santos, Éverton |
spellingShingle |
da Silva Santos, Éverton misc Candidiasis misc Cactaceae misc Cactus misc Phenethylamines misc Tetrahydroisoquinoline misc Tissue culture UHPLC-ESI Analysis and Antifungal Activity of Cladodes and Callus Cultures of Cereus hildmannianus |
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UHPLC-ESI Analysis and Antifungal Activity of Cladodes and Callus Cultures of Cereus hildmannianus Candidiasis (dpeaa)DE-He213 Cactaceae (dpeaa)DE-He213 Cactus (dpeaa)DE-He213 Phenethylamines (dpeaa)DE-He213 Tetrahydroisoquinoline (dpeaa)DE-He213 Tissue culture (dpeaa)DE-He213 |
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misc Candidiasis misc Cactaceae misc Cactus misc Phenethylamines misc Tetrahydroisoquinoline misc Tissue culture |
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UHPLC-ESI Analysis and Antifungal Activity of Cladodes and Callus Cultures of Cereus hildmannianus |
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UHPLC-ESI Analysis and Antifungal Activity of Cladodes and Callus Cultures of Cereus hildmannianus |
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da Silva Santos, Éverton Savam, Aline Arita, Glaucia Sayuri Cabral, Márcia Regina Pereira Bonfim-Mendonça, Patrícia Souza de Fatima Pires Silva Machado, Maria de Oliveira, Arildo José Braz Gonçalves, Regina Aparecida Correia |
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title_sort |
uhplc-esi analysis and antifungal activity of cladodes and callus cultures of cereus hildmannianus |
title_auth |
UHPLC-ESI Analysis and Antifungal Activity of Cladodes and Callus Cultures of Cereus hildmannianus |
abstract |
Plant biotechnology has a great potential in providing bioactive compounds with pharmacological applications. Bioactive compounds have been highlighted as an alternative antifungal treatment option due to the limited number of antifungals currently available. The aim of this study was to evaluate the antifungal activity and chemical profile of Cereus hildmannianus K.Schum., Cactaceae, cladodes and callus cultures. The cladodes of wild plants were collected, and the conventional callus was cultivated using agar and Murashige and Skoog medium, while the alternative culture was cultivated using a cotton support covered with filter paper and the same medium (incubated at 32 °C with 16-h photoperiod). Ethanolic extracts were prepared by Soxhlet and chemically characterized by ultra-high-performance liquid chromatography coupled with electrospray ionization quadrupole time-of-flight tandem mass spectrometry (UHPLC-ESI-QToF-MS/MS). The antifungal activity was evaluated against Candida glabrata, C. parapsilosis, C. tropicalis, and C. albicans by broth microdilution. The UHPLC-ESI-QToF-MS/MS method enabled the putative identification of fifteen proto-alkaloids and alkaloids, with pellotine and mescaline described for the first time in this species. The antifungal activity of each extract was species-dependent; extracts from both the conventional and alternative callus cultures were prominent against C. glabrata (minimal inhibitory concentration of 312 µg/ml), but there was also potential inhibitory activity against other Candida species. Graphical Abstract © The Author(s) under exclusive licence to Sociedade Brasileira de Farmacognosia 2023 |
abstractGer |
Plant biotechnology has a great potential in providing bioactive compounds with pharmacological applications. Bioactive compounds have been highlighted as an alternative antifungal treatment option due to the limited number of antifungals currently available. The aim of this study was to evaluate the antifungal activity and chemical profile of Cereus hildmannianus K.Schum., Cactaceae, cladodes and callus cultures. The cladodes of wild plants were collected, and the conventional callus was cultivated using agar and Murashige and Skoog medium, while the alternative culture was cultivated using a cotton support covered with filter paper and the same medium (incubated at 32 °C with 16-h photoperiod). Ethanolic extracts were prepared by Soxhlet and chemically characterized by ultra-high-performance liquid chromatography coupled with electrospray ionization quadrupole time-of-flight tandem mass spectrometry (UHPLC-ESI-QToF-MS/MS). The antifungal activity was evaluated against Candida glabrata, C. parapsilosis, C. tropicalis, and C. albicans by broth microdilution. The UHPLC-ESI-QToF-MS/MS method enabled the putative identification of fifteen proto-alkaloids and alkaloids, with pellotine and mescaline described for the first time in this species. The antifungal activity of each extract was species-dependent; extracts from both the conventional and alternative callus cultures were prominent against C. glabrata (minimal inhibitory concentration of 312 µg/ml), but there was also potential inhibitory activity against other Candida species. Graphical Abstract © The Author(s) under exclusive licence to Sociedade Brasileira de Farmacognosia 2023 |
abstract_unstemmed |
Plant biotechnology has a great potential in providing bioactive compounds with pharmacological applications. Bioactive compounds have been highlighted as an alternative antifungal treatment option due to the limited number of antifungals currently available. The aim of this study was to evaluate the antifungal activity and chemical profile of Cereus hildmannianus K.Schum., Cactaceae, cladodes and callus cultures. The cladodes of wild plants were collected, and the conventional callus was cultivated using agar and Murashige and Skoog medium, while the alternative culture was cultivated using a cotton support covered with filter paper and the same medium (incubated at 32 °C with 16-h photoperiod). Ethanolic extracts were prepared by Soxhlet and chemically characterized by ultra-high-performance liquid chromatography coupled with electrospray ionization quadrupole time-of-flight tandem mass spectrometry (UHPLC-ESI-QToF-MS/MS). The antifungal activity was evaluated against Candida glabrata, C. parapsilosis, C. tropicalis, and C. albicans by broth microdilution. The UHPLC-ESI-QToF-MS/MS method enabled the putative identification of fifteen proto-alkaloids and alkaloids, with pellotine and mescaline described for the first time in this species. The antifungal activity of each extract was species-dependent; extracts from both the conventional and alternative callus cultures were prominent against C. glabrata (minimal inhibitory concentration of 312 µg/ml), but there was also potential inhibitory activity against other Candida species. Graphical Abstract © The Author(s) under exclusive licence to Sociedade Brasileira de Farmacognosia 2023 |
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container_issue |
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title_short |
UHPLC-ESI Analysis and Antifungal Activity of Cladodes and Callus Cultures of Cereus hildmannianus |
url |
https://dx.doi.org/10.1007/s43450-023-00418-2 |
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author2 |
Savam, Aline Arita, Glaucia Sayuri Cabral, Márcia Regina Pereira Bonfim-Mendonça, Patrícia Souza de Fatima Pires Silva Machado, Maria de Oliveira, Arildo José Braz Gonçalves, Regina Aparecida Correia |
author2Str |
Savam, Aline Arita, Glaucia Sayuri Cabral, Márcia Regina Pereira Bonfim-Mendonça, Patrícia Souza de Fatima Pires Silva Machado, Maria de Oliveira, Arildo José Braz Gonçalves, Regina Aparecida Correia |
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doi_str |
10.1007/s43450-023-00418-2 |
up_date |
2024-07-03T15:51:15.505Z |
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score |
7.4010077 |