Development and application of classical swine fever virus monoclonal antibodies derived from single B cells

Abstract Vaccination with E2 subunit vaccines is currently the main measure to control classical swine fever virus (CSFV), which is an endemic disease, and detection of antibodies against CSFV E2 is the most effective way to evaluate herd immunity. In the present study, the E2 protein was expressed...
Ausführliche Beschreibung

Gespeichert in:

Autor*in:	Ma, Zhongyuan [verfasserIn] Zhao, Yongcong Lv, Jianliang Pan, Li

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2023

Schlagwörter:	CSFV E2 single B cells epitope bELISA

Anmerkung:	© The Author(s) 2023

Übergeordnetes Werk:	Enthalten in: Veterinary research - London : BioMed Central, 1993, 54(2023), 1 vom: 16. Okt.
Übergeordnetes Werk:	volume:54 ; year:2023 ; number:1 ; day:16 ; month:10

Links:	Volltext

DOI / URN:	10.1186/s13567-023-01229-y

Katalog-ID:	SPR053426037

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520			\|a Abstract Vaccination with E2 subunit vaccines is currently the main measure to control classical swine fever virus (CSFV), which is an endemic disease, and detection of antibodies against CSFV E2 is the most effective way to evaluate herd immunity. In the present study, the E2 protein was expressed by a baculovirus expression system, and two monoclonal antibodies (mAbs), namely, 3A9 and 4F7, were successfully produced using techniques for the isolation of single B cells from splenocytes from mice immunized with the E2 protein. Moreover, two linear B-cell epitopes, 25$ GLTTTWKEYSHDLQL^{39} $ and 259$ GNTTVKVHASDERGP^{273} $, reactive to 3A9 and 4F7, respectively, were identified using epitope mapping of the E2 protein. In addition, the diagnostic performance of the two mAbs was evaluated using blocking enzyme-linked immunosorbent assay (bELISA), and the results showed that the two mAbs had high diagnostic specificity (96.08%, 94.38%) and diagnostic sensitivity (97.49%, 95.97%). Together, these findings identify two ideal candidate peptides and matching mAbs for a new method of CSFV diagnosis, which will contribute to the control and eradication of classical swine fever.
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allfields	10.1186/s13567-023-01229-y doi (DE-627)SPR053426037 (SPR)s13567-023-01229-y-e DE-627 ger DE-627 rakwb eng Ma, Zhongyuan verfasserin aut Development and application of classical swine fever virus monoclonal antibodies derived from single B cells 2023 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s) 2023 Abstract Vaccination with E2 subunit vaccines is currently the main measure to control classical swine fever virus (CSFV), which is an endemic disease, and detection of antibodies against CSFV E2 is the most effective way to evaluate herd immunity. In the present study, the E2 protein was expressed by a baculovirus expression system, and two monoclonal antibodies (mAbs), namely, 3A9 and 4F7, were successfully produced using techniques for the isolation of single B cells from splenocytes from mice immunized with the E2 protein. Moreover, two linear B-cell epitopes, 25$ GLTTTWKEYSHDLQL^{39} $ and 259$ GNTTVKVHASDERGP^{273} $, reactive to 3A9 and 4F7, respectively, were identified using epitope mapping of the E2 protein. In addition, the diagnostic performance of the two mAbs was evaluated using blocking enzyme-linked immunosorbent assay (bELISA), and the results showed that the two mAbs had high diagnostic specificity (96.08%, 94.38%) and diagnostic sensitivity (97.49%, 95.97%). Together, these findings identify two ideal candidate peptides and matching mAbs for a new method of CSFV diagnosis, which will contribute to the control and eradication of classical swine fever. CSFV (dpeaa)DE-He213 E2 (dpeaa)DE-He213 single B cells (dpeaa)DE-He213 epitope (dpeaa)DE-He213 bELISA (dpeaa)DE-He213 Zhao, Yongcong aut Lv, Jianliang aut Pan, Li aut Enthalten in Veterinary research London : BioMed Central, 1993 54(2023), 1 vom: 16. Okt. (DE-627)312853653 (DE-600)2012391-7 1297-9716 nnns volume:54 year:2023 number:1 day:16 month:10 https://dx.doi.org/10.1186/s13567-023-01229-y kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_213 GBV_ILN_230 GBV_ILN_252 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 54 2023 1 16 10
spelling	10.1186/s13567-023-01229-y doi (DE-627)SPR053426037 (SPR)s13567-023-01229-y-e DE-627 ger DE-627 rakwb eng Ma, Zhongyuan verfasserin aut Development and application of classical swine fever virus monoclonal antibodies derived from single B cells 2023 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s) 2023 Abstract Vaccination with E2 subunit vaccines is currently the main measure to control classical swine fever virus (CSFV), which is an endemic disease, and detection of antibodies against CSFV E2 is the most effective way to evaluate herd immunity. In the present study, the E2 protein was expressed by a baculovirus expression system, and two monoclonal antibodies (mAbs), namely, 3A9 and 4F7, were successfully produced using techniques for the isolation of single B cells from splenocytes from mice immunized with the E2 protein. Moreover, two linear B-cell epitopes, 25$ GLTTTWKEYSHDLQL^{39} $ and 259$ GNTTVKVHASDERGP^{273} $, reactive to 3A9 and 4F7, respectively, were identified using epitope mapping of the E2 protein. In addition, the diagnostic performance of the two mAbs was evaluated using blocking enzyme-linked immunosorbent assay (bELISA), and the results showed that the two mAbs had high diagnostic specificity (96.08%, 94.38%) and diagnostic sensitivity (97.49%, 95.97%). Together, these findings identify two ideal candidate peptides and matching mAbs for a new method of CSFV diagnosis, which will contribute to the control and eradication of classical swine fever. CSFV (dpeaa)DE-He213 E2 (dpeaa)DE-He213 single B cells (dpeaa)DE-He213 epitope (dpeaa)DE-He213 bELISA (dpeaa)DE-He213 Zhao, Yongcong aut Lv, Jianliang aut Pan, Li aut Enthalten in Veterinary research London : BioMed Central, 1993 54(2023), 1 vom: 16. Okt. (DE-627)312853653 (DE-600)2012391-7 1297-9716 nnns volume:54 year:2023 number:1 day:16 month:10 https://dx.doi.org/10.1186/s13567-023-01229-y kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_213 GBV_ILN_230 GBV_ILN_252 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 54 2023 1 16 10
allfields_unstemmed	10.1186/s13567-023-01229-y doi (DE-627)SPR053426037 (SPR)s13567-023-01229-y-e DE-627 ger DE-627 rakwb eng Ma, Zhongyuan verfasserin aut Development and application of classical swine fever virus monoclonal antibodies derived from single B cells 2023 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s) 2023 Abstract Vaccination with E2 subunit vaccines is currently the main measure to control classical swine fever virus (CSFV), which is an endemic disease, and detection of antibodies against CSFV E2 is the most effective way to evaluate herd immunity. In the present study, the E2 protein was expressed by a baculovirus expression system, and two monoclonal antibodies (mAbs), namely, 3A9 and 4F7, were successfully produced using techniques for the isolation of single B cells from splenocytes from mice immunized with the E2 protein. Moreover, two linear B-cell epitopes, 25$ GLTTTWKEYSHDLQL^{39} $ and 259$ GNTTVKVHASDERGP^{273} $, reactive to 3A9 and 4F7, respectively, were identified using epitope mapping of the E2 protein. In addition, the diagnostic performance of the two mAbs was evaluated using blocking enzyme-linked immunosorbent assay (bELISA), and the results showed that the two mAbs had high diagnostic specificity (96.08%, 94.38%) and diagnostic sensitivity (97.49%, 95.97%). Together, these findings identify two ideal candidate peptides and matching mAbs for a new method of CSFV diagnosis, which will contribute to the control and eradication of classical swine fever. CSFV (dpeaa)DE-He213 E2 (dpeaa)DE-He213 single B cells (dpeaa)DE-He213 epitope (dpeaa)DE-He213 bELISA (dpeaa)DE-He213 Zhao, Yongcong aut Lv, Jianliang aut Pan, Li aut Enthalten in Veterinary research London : BioMed Central, 1993 54(2023), 1 vom: 16. Okt. (DE-627)312853653 (DE-600)2012391-7 1297-9716 nnns volume:54 year:2023 number:1 day:16 month:10 https://dx.doi.org/10.1186/s13567-023-01229-y kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_213 GBV_ILN_230 GBV_ILN_252 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 54 2023 1 16 10
allfieldsGer	10.1186/s13567-023-01229-y doi (DE-627)SPR053426037 (SPR)s13567-023-01229-y-e DE-627 ger DE-627 rakwb eng Ma, Zhongyuan verfasserin aut Development and application of classical swine fever virus monoclonal antibodies derived from single B cells 2023 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s) 2023 Abstract Vaccination with E2 subunit vaccines is currently the main measure to control classical swine fever virus (CSFV), which is an endemic disease, and detection of antibodies against CSFV E2 is the most effective way to evaluate herd immunity. In the present study, the E2 protein was expressed by a baculovirus expression system, and two monoclonal antibodies (mAbs), namely, 3A9 and 4F7, were successfully produced using techniques for the isolation of single B cells from splenocytes from mice immunized with the E2 protein. Moreover, two linear B-cell epitopes, 25$ GLTTTWKEYSHDLQL^{39} $ and 259$ GNTTVKVHASDERGP^{273} $, reactive to 3A9 and 4F7, respectively, were identified using epitope mapping of the E2 protein. In addition, the diagnostic performance of the two mAbs was evaluated using blocking enzyme-linked immunosorbent assay (bELISA), and the results showed that the two mAbs had high diagnostic specificity (96.08%, 94.38%) and diagnostic sensitivity (97.49%, 95.97%). Together, these findings identify two ideal candidate peptides and matching mAbs for a new method of CSFV diagnosis, which will contribute to the control and eradication of classical swine fever. CSFV (dpeaa)DE-He213 E2 (dpeaa)DE-He213 single B cells (dpeaa)DE-He213 epitope (dpeaa)DE-He213 bELISA (dpeaa)DE-He213 Zhao, Yongcong aut Lv, Jianliang aut Pan, Li aut Enthalten in Veterinary research London : BioMed Central, 1993 54(2023), 1 vom: 16. Okt. (DE-627)312853653 (DE-600)2012391-7 1297-9716 nnns volume:54 year:2023 number:1 day:16 month:10 https://dx.doi.org/10.1186/s13567-023-01229-y kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_213 GBV_ILN_230 GBV_ILN_252 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 54 2023 1 16 10
allfieldsSound	10.1186/s13567-023-01229-y doi (DE-627)SPR053426037 (SPR)s13567-023-01229-y-e DE-627 ger DE-627 rakwb eng Ma, Zhongyuan verfasserin aut Development and application of classical swine fever virus monoclonal antibodies derived from single B cells 2023 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s) 2023 Abstract Vaccination with E2 subunit vaccines is currently the main measure to control classical swine fever virus (CSFV), which is an endemic disease, and detection of antibodies against CSFV E2 is the most effective way to evaluate herd immunity. In the present study, the E2 protein was expressed by a baculovirus expression system, and two monoclonal antibodies (mAbs), namely, 3A9 and 4F7, were successfully produced using techniques for the isolation of single B cells from splenocytes from mice immunized with the E2 protein. Moreover, two linear B-cell epitopes, 25$ GLTTTWKEYSHDLQL^{39} $ and 259$ GNTTVKVHASDERGP^{273} $, reactive to 3A9 and 4F7, respectively, were identified using epitope mapping of the E2 protein. In addition, the diagnostic performance of the two mAbs was evaluated using blocking enzyme-linked immunosorbent assay (bELISA), and the results showed that the two mAbs had high diagnostic specificity (96.08%, 94.38%) and diagnostic sensitivity (97.49%, 95.97%). Together, these findings identify two ideal candidate peptides and matching mAbs for a new method of CSFV diagnosis, which will contribute to the control and eradication of classical swine fever. CSFV (dpeaa)DE-He213 E2 (dpeaa)DE-He213 single B cells (dpeaa)DE-He213 epitope (dpeaa)DE-He213 bELISA (dpeaa)DE-He213 Zhao, Yongcong aut Lv, Jianliang aut Pan, Li aut Enthalten in Veterinary research London : BioMed Central, 1993 54(2023), 1 vom: 16. Okt. (DE-627)312853653 (DE-600)2012391-7 1297-9716 nnns volume:54 year:2023 number:1 day:16 month:10 https://dx.doi.org/10.1186/s13567-023-01229-y kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_213 GBV_ILN_230 GBV_ILN_252 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 54 2023 1 16 10
language	English
source	Enthalten in Veterinary research 54(2023), 1 vom: 16. Okt. volume:54 year:2023 number:1 day:16 month:10
sourceStr	Enthalten in Veterinary research 54(2023), 1 vom: 16. Okt. volume:54 year:2023 number:1 day:16 month:10
format_phy_str_mv	Article
institution	findex.gbv.de
topic_facet	CSFV E2 single B cells epitope bELISA
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container_title	Veterinary research
authorswithroles_txt_mv	Ma, Zhongyuan @@aut@@ Zhao, Yongcong @@aut@@ Lv, Jianliang @@aut@@ Pan, Li @@aut@@
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author	Ma, Zhongyuan
spellingShingle	Ma, Zhongyuan misc CSFV misc E2 misc single B cells misc epitope misc bELISA Development and application of classical swine fever virus monoclonal antibodies derived from single B cells
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topic_title	Development and application of classical swine fever virus monoclonal antibodies derived from single B cells CSFV (dpeaa)DE-He213 E2 (dpeaa)DE-He213 single B cells (dpeaa)DE-He213 epitope (dpeaa)DE-He213 bELISA (dpeaa)DE-He213
topic	misc CSFV misc E2 misc single B cells misc epitope misc bELISA
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title	Development and application of classical swine fever virus monoclonal antibodies derived from single B cells
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title_full	Development and application of classical swine fever virus monoclonal antibodies derived from single B cells
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author_browse	Ma, Zhongyuan Zhao, Yongcong Lv, Jianliang Pan, Li
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doi_str_mv	10.1186/s13567-023-01229-y
title_sort	development and application of classical swine fever virus monoclonal antibodies derived from single b cells
title_auth	Development and application of classical swine fever virus monoclonal antibodies derived from single B cells
abstract	Abstract Vaccination with E2 subunit vaccines is currently the main measure to control classical swine fever virus (CSFV), which is an endemic disease, and detection of antibodies against CSFV E2 is the most effective way to evaluate herd immunity. In the present study, the E2 protein was expressed by a baculovirus expression system, and two monoclonal antibodies (mAbs), namely, 3A9 and 4F7, were successfully produced using techniques for the isolation of single B cells from splenocytes from mice immunized with the E2 protein. Moreover, two linear B-cell epitopes, 25$ GLTTTWKEYSHDLQL^{39} $ and 259$ GNTTVKVHASDERGP^{273} $, reactive to 3A9 and 4F7, respectively, were identified using epitope mapping of the E2 protein. In addition, the diagnostic performance of the two mAbs was evaluated using blocking enzyme-linked immunosorbent assay (bELISA), and the results showed that the two mAbs had high diagnostic specificity (96.08%, 94.38%) and diagnostic sensitivity (97.49%, 95.97%). Together, these findings identify two ideal candidate peptides and matching mAbs for a new method of CSFV diagnosis, which will contribute to the control and eradication of classical swine fever. © The Author(s) 2023
abstractGer	Abstract Vaccination with E2 subunit vaccines is currently the main measure to control classical swine fever virus (CSFV), which is an endemic disease, and detection of antibodies against CSFV E2 is the most effective way to evaluate herd immunity. In the present study, the E2 protein was expressed by a baculovirus expression system, and two monoclonal antibodies (mAbs), namely, 3A9 and 4F7, were successfully produced using techniques for the isolation of single B cells from splenocytes from mice immunized with the E2 protein. Moreover, two linear B-cell epitopes, 25$ GLTTTWKEYSHDLQL^{39} $ and 259$ GNTTVKVHASDERGP^{273} $, reactive to 3A9 and 4F7, respectively, were identified using epitope mapping of the E2 protein. In addition, the diagnostic performance of the two mAbs was evaluated using blocking enzyme-linked immunosorbent assay (bELISA), and the results showed that the two mAbs had high diagnostic specificity (96.08%, 94.38%) and diagnostic sensitivity (97.49%, 95.97%). Together, these findings identify two ideal candidate peptides and matching mAbs for a new method of CSFV diagnosis, which will contribute to the control and eradication of classical swine fever. © The Author(s) 2023
abstract_unstemmed	Abstract Vaccination with E2 subunit vaccines is currently the main measure to control classical swine fever virus (CSFV), which is an endemic disease, and detection of antibodies against CSFV E2 is the most effective way to evaluate herd immunity. In the present study, the E2 protein was expressed by a baculovirus expression system, and two monoclonal antibodies (mAbs), namely, 3A9 and 4F7, were successfully produced using techniques for the isolation of single B cells from splenocytes from mice immunized with the E2 protein. Moreover, two linear B-cell epitopes, 25$ GLTTTWKEYSHDLQL^{39} $ and 259$ GNTTVKVHASDERGP^{273} $, reactive to 3A9 and 4F7, respectively, were identified using epitope mapping of the E2 protein. In addition, the diagnostic performance of the two mAbs was evaluated using blocking enzyme-linked immunosorbent assay (bELISA), and the results showed that the two mAbs had high diagnostic specificity (96.08%, 94.38%) and diagnostic sensitivity (97.49%, 95.97%). Together, these findings identify two ideal candidate peptides and matching mAbs for a new method of CSFV diagnosis, which will contribute to the control and eradication of classical swine fever. © The Author(s) 2023
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title_short	Development and application of classical swine fever virus monoclonal antibodies derived from single B cells
url	https://dx.doi.org/10.1186/s13567-023-01229-y
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author2	Zhao, Yongcong Lv, Jianliang Pan, Li
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up_date	2024-07-03T19:26:51.159Z
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Development and application of classical swine fever virus monoclonal antibodies derived from single B cells

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