In-vitro model to mimic T cell subset change in human PDAC organoid co-culture

Purpose Immunotherapies have largely failed as treatment options for pancreatic ductal adenocarcinoma (PDAC). In this field, clinical translational studies into personalized treatment are of fundamental importance. In our study, we model tumor-cell immune-cell interactions in a co-culture of primary...
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Autor*in:	Knoblauch, M. [verfasserIn] Ma, T. Beirith, I. Koch, D. Hofmann, F. Heinrich, K. Aghamaliev, U. Sirtl, S. Westphalen, C. B. Nieß, H. Reichert, M. Angele, M. K. Regel, I. Bazhin, A. V. Werner, J. Ilmer, M. Renz, Bernhard W.

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2023

Schlagwörter:	Pancreatic cancer Organoids Tumor-immune interaction Pancreatic cancer organoids

Anmerkung:	© The Author(s) 2023

Übergeordnetes Werk:	Enthalten in: Journal of cancer research and clinical oncology - Berlin : Springer, 1904, 149(2023), 14 vom: 20. Juli, Seite 13051-13064
Übergeordnetes Werk:	volume:149 ; year:2023 ; number:14 ; day:20 ; month:07 ; pages:13051-13064

Links:	Volltext

DOI / URN:	10.1007/s00432-023-05100-7

Katalog-ID:	SPR053456416

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245	1	0	\|a In-vitro model to mimic T cell subset change in human PDAC organoid co-culture
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520			\|a Purpose Immunotherapies have largely failed as treatment options for pancreatic ductal adenocarcinoma (PDAC). In this field, clinical translational studies into personalized treatment are of fundamental importance. In our study, we model tumor-cell immune-cell interactions in a co-culture of primary human PDAC organoids and matched peripheral blood mononuclear cells (PBMCs). Methods Using flow cytometry, we evaluated changes in T cell subtypes upon co-culture of patient-derived PDAC organoids and matched PBMCs. Results After co-culturing PDAC organoids with PBMCs, we observed changes in $ CD4^{+} $, $ CD8^{+} $ and Treg cell populations. We observed favorable clinical outcome in patients whose PBMCs reacted to the co-culture with organoids. Conclusion This experimental model allows to investigate interactions between patient derived PDAC organoids and their PBMCs. This co-culture system could serve as a preclinical platform to guide personalized therapeutic strategies in the future.
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650		4	\|a Pancreatic cancer organoids \|7 (dpeaa)DE-He213
700	1		\|a Ma, T. \|4 aut
700	1		\|a Beirith, I. \|4 aut
700	1		\|a Koch, D. \|4 aut
700	1		\|a Hofmann, F. \|4 aut
700	1		\|a Heinrich, K. \|4 aut
700	1		\|a Aghamaliev, U. \|4 aut
700	1		\|a Sirtl, S. \|4 aut
700	1		\|a Westphalen, C. B. \|4 aut
700	1		\|a Nieß, H. \|4 aut
700	1		\|a Reichert, M. \|4 aut
700	1		\|a Angele, M. K. \|4 aut
700	1		\|a Regel, I. \|4 aut
700	1		\|a Bazhin, A. V. \|4 aut
700	1		\|a Werner, J. \|4 aut
700	1		\|a Ilmer, M. \|4 aut
700	1		\|a Renz, Bernhard W. \|4 aut
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912			\|a GBV_ILN_70
912			\|a GBV_ILN_73
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912			\|a GBV_ILN_90
912			\|a GBV_ILN_95
912			\|a GBV_ILN_100
912			\|a GBV_ILN_101
912			\|a GBV_ILN_105
912			\|a GBV_ILN_110
912			\|a GBV_ILN_120
912			\|a GBV_ILN_138
912			\|a GBV_ILN_150
912			\|a GBV_ILN_151
912			\|a GBV_ILN_152
912			\|a GBV_ILN_161
912			\|a GBV_ILN_165
912			\|a GBV_ILN_170
912			\|a GBV_ILN_171
912			\|a GBV_ILN_187
912			\|a GBV_ILN_213
912			\|a GBV_ILN_224
912			\|a GBV_ILN_230
912			\|a GBV_ILN_250
912			\|a GBV_ILN_267
912			\|a GBV_ILN_281
912			\|a GBV_ILN_285
912			\|a GBV_ILN_293
912			\|a GBV_ILN_370
912			\|a GBV_ILN_602
912			\|a GBV_ILN_636
912			\|a GBV_ILN_702
912			\|a GBV_ILN_711
912			\|a GBV_ILN_2001
912			\|a GBV_ILN_2003
912			\|a GBV_ILN_2004
912			\|a GBV_ILN_2005
912			\|a GBV_ILN_2006
912			\|a GBV_ILN_2007
912			\|a GBV_ILN_2008
912			\|a GBV_ILN_2009
912			\|a GBV_ILN_2010
912			\|a GBV_ILN_2011
912			\|a GBV_ILN_2014
912			\|a GBV_ILN_2015
912			\|a GBV_ILN_2020
912			\|a GBV_ILN_2021
912			\|a GBV_ILN_2025
912			\|a GBV_ILN_2026
912			\|a GBV_ILN_2027
912			\|a GBV_ILN_2031
912			\|a GBV_ILN_2034
912			\|a GBV_ILN_2037
912			\|a GBV_ILN_2038
912			\|a GBV_ILN_2039
912			\|a GBV_ILN_2044
912			\|a GBV_ILN_2048
912			\|a GBV_ILN_2049
912			\|a GBV_ILN_2050
912			\|a GBV_ILN_2055
912			\|a GBV_ILN_2056
912			\|a GBV_ILN_2057
912			\|a GBV_ILN_2059
912			\|a GBV_ILN_2061
912			\|a GBV_ILN_2064
912			\|a GBV_ILN_2065
912			\|a GBV_ILN_2068
912			\|a GBV_ILN_2088
912			\|a GBV_ILN_2093
912			\|a GBV_ILN_2106
912			\|a GBV_ILN_2107
912			\|a GBV_ILN_2108
912			\|a GBV_ILN_2110
912			\|a GBV_ILN_2111
912			\|a GBV_ILN_2112
912			\|a GBV_ILN_2113
912			\|a GBV_ILN_2118
912			\|a GBV_ILN_2122
912			\|a GBV_ILN_2129
912			\|a GBV_ILN_2143
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912			\|a GBV_ILN_2152
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912			\|a GBV_ILN_2190
912			\|a GBV_ILN_2232
912			\|a GBV_ILN_2336
912			\|a GBV_ILN_2446
912			\|a GBV_ILN_2470
912			\|a GBV_ILN_2472
912			\|a GBV_ILN_2507
912			\|a GBV_ILN_2522
912			\|a GBV_ILN_4035
912			\|a GBV_ILN_4037
912			\|a GBV_ILN_4046
912			\|a GBV_ILN_4112
912			\|a GBV_ILN_4125
912			\|a GBV_ILN_4126
912			\|a GBV_ILN_4242
912			\|a GBV_ILN_4246
912			\|a GBV_ILN_4249
912			\|a GBV_ILN_4251
912			\|a GBV_ILN_4305
912			\|a GBV_ILN_4306
912			\|a GBV_ILN_4307
912			\|a GBV_ILN_4313
912			\|a GBV_ILN_4322
912			\|a GBV_ILN_4323
912			\|a GBV_ILN_4324
912			\|a GBV_ILN_4325
912			\|a GBV_ILN_4326
912			\|a GBV_ILN_4328
912			\|a GBV_ILN_4333
912			\|a GBV_ILN_4334
912			\|a GBV_ILN_4335
912			\|a GBV_ILN_4336
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author_variant	m k mk t m tm i b ib d k dk f h fh k h kh u a ua s s ss c b w cb cbw h n hn m r mr m k a mk mka i r ir a v b av avb j w jw m i mi b w r bw bwr
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publishDate	2023
allfields	10.1007/s00432-023-05100-7 doi (DE-627)SPR053456416 (SPR)s00432-023-05100-7-e DE-627 ger DE-627 rakwb eng Knoblauch, M. verfasserin aut In-vitro model to mimic T cell subset change in human PDAC organoid co-culture 2023 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s) 2023 Purpose Immunotherapies have largely failed as treatment options for pancreatic ductal adenocarcinoma (PDAC). In this field, clinical translational studies into personalized treatment are of fundamental importance. In our study, we model tumor-cell immune-cell interactions in a co-culture of primary human PDAC organoids and matched peripheral blood mononuclear cells (PBMCs). Methods Using flow cytometry, we evaluated changes in T cell subtypes upon co-culture of patient-derived PDAC organoids and matched PBMCs. Results After co-culturing PDAC organoids with PBMCs, we observed changes in $ CD4^{+} $, $ CD8^{+} $ and Treg cell populations. We observed favorable clinical outcome in patients whose PBMCs reacted to the co-culture with organoids. Conclusion This experimental model allows to investigate interactions between patient derived PDAC organoids and their PBMCs. This co-culture system could serve as a preclinical platform to guide personalized therapeutic strategies in the future. Pancreatic cancer (dpeaa)DE-He213 Organoids (dpeaa)DE-He213 Tumor-immune interaction (dpeaa)DE-He213 Pancreatic cancer organoids (dpeaa)DE-He213 Ma, T. aut Beirith, I. aut Koch, D. aut Hofmann, F. aut Heinrich, K. aut Aghamaliev, U. aut Sirtl, S. aut Westphalen, C. B. aut Nieß, H. aut Reichert, M. aut Angele, M. K. aut Regel, I. aut Bazhin, A. V. aut Werner, J. aut Ilmer, M. aut Renz, Bernhard W. aut Enthalten in Journal of cancer research and clinical oncology Berlin : Springer, 1904 149(2023), 14 vom: 20. Juli, Seite 13051-13064 (DE-627)253769515 (DE-600)1459285-X 1432-1335 nnns volume:149 year:2023 number:14 day:20 month:07 pages:13051-13064 https://dx.doi.org/10.1007/s00432-023-05100-7 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_165 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_267 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_711 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 149 2023 14 20 07 13051-13064
spelling	10.1007/s00432-023-05100-7 doi (DE-627)SPR053456416 (SPR)s00432-023-05100-7-e DE-627 ger DE-627 rakwb eng Knoblauch, M. verfasserin aut In-vitro model to mimic T cell subset change in human PDAC organoid co-culture 2023 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s) 2023 Purpose Immunotherapies have largely failed as treatment options for pancreatic ductal adenocarcinoma (PDAC). In this field, clinical translational studies into personalized treatment are of fundamental importance. In our study, we model tumor-cell immune-cell interactions in a co-culture of primary human PDAC organoids and matched peripheral blood mononuclear cells (PBMCs). Methods Using flow cytometry, we evaluated changes in T cell subtypes upon co-culture of patient-derived PDAC organoids and matched PBMCs. Results After co-culturing PDAC organoids with PBMCs, we observed changes in $ CD4^{+} $, $ CD8^{+} $ and Treg cell populations. We observed favorable clinical outcome in patients whose PBMCs reacted to the co-culture with organoids. Conclusion This experimental model allows to investigate interactions between patient derived PDAC organoids and their PBMCs. This co-culture system could serve as a preclinical platform to guide personalized therapeutic strategies in the future. Pancreatic cancer (dpeaa)DE-He213 Organoids (dpeaa)DE-He213 Tumor-immune interaction (dpeaa)DE-He213 Pancreatic cancer organoids (dpeaa)DE-He213 Ma, T. aut Beirith, I. aut Koch, D. aut Hofmann, F. aut Heinrich, K. aut Aghamaliev, U. aut Sirtl, S. aut Westphalen, C. B. aut Nieß, H. aut Reichert, M. aut Angele, M. K. aut Regel, I. aut Bazhin, A. V. aut Werner, J. aut Ilmer, M. aut Renz, Bernhard W. aut Enthalten in Journal of cancer research and clinical oncology Berlin : Springer, 1904 149(2023), 14 vom: 20. Juli, Seite 13051-13064 (DE-627)253769515 (DE-600)1459285-X 1432-1335 nnns volume:149 year:2023 number:14 day:20 month:07 pages:13051-13064 https://dx.doi.org/10.1007/s00432-023-05100-7 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_165 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_267 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_711 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 149 2023 14 20 07 13051-13064
allfields_unstemmed	10.1007/s00432-023-05100-7 doi (DE-627)SPR053456416 (SPR)s00432-023-05100-7-e DE-627 ger DE-627 rakwb eng Knoblauch, M. verfasserin aut In-vitro model to mimic T cell subset change in human PDAC organoid co-culture 2023 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s) 2023 Purpose Immunotherapies have largely failed as treatment options for pancreatic ductal adenocarcinoma (PDAC). In this field, clinical translational studies into personalized treatment are of fundamental importance. In our study, we model tumor-cell immune-cell interactions in a co-culture of primary human PDAC organoids and matched peripheral blood mononuclear cells (PBMCs). Methods Using flow cytometry, we evaluated changes in T cell subtypes upon co-culture of patient-derived PDAC organoids and matched PBMCs. Results After co-culturing PDAC organoids with PBMCs, we observed changes in $ CD4^{+} $, $ CD8^{+} $ and Treg cell populations. We observed favorable clinical outcome in patients whose PBMCs reacted to the co-culture with organoids. Conclusion This experimental model allows to investigate interactions between patient derived PDAC organoids and their PBMCs. This co-culture system could serve as a preclinical platform to guide personalized therapeutic strategies in the future. Pancreatic cancer (dpeaa)DE-He213 Organoids (dpeaa)DE-He213 Tumor-immune interaction (dpeaa)DE-He213 Pancreatic cancer organoids (dpeaa)DE-He213 Ma, T. aut Beirith, I. aut Koch, D. aut Hofmann, F. aut Heinrich, K. aut Aghamaliev, U. aut Sirtl, S. aut Westphalen, C. B. aut Nieß, H. aut Reichert, M. aut Angele, M. K. aut Regel, I. aut Bazhin, A. V. aut Werner, J. aut Ilmer, M. aut Renz, Bernhard W. aut Enthalten in Journal of cancer research and clinical oncology Berlin : Springer, 1904 149(2023), 14 vom: 20. Juli, Seite 13051-13064 (DE-627)253769515 (DE-600)1459285-X 1432-1335 nnns volume:149 year:2023 number:14 day:20 month:07 pages:13051-13064 https://dx.doi.org/10.1007/s00432-023-05100-7 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_165 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_267 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_711 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 149 2023 14 20 07 13051-13064
allfieldsGer	10.1007/s00432-023-05100-7 doi (DE-627)SPR053456416 (SPR)s00432-023-05100-7-e DE-627 ger DE-627 rakwb eng Knoblauch, M. verfasserin aut In-vitro model to mimic T cell subset change in human PDAC organoid co-culture 2023 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s) 2023 Purpose Immunotherapies have largely failed as treatment options for pancreatic ductal adenocarcinoma (PDAC). In this field, clinical translational studies into personalized treatment are of fundamental importance. In our study, we model tumor-cell immune-cell interactions in a co-culture of primary human PDAC organoids and matched peripheral blood mononuclear cells (PBMCs). Methods Using flow cytometry, we evaluated changes in T cell subtypes upon co-culture of patient-derived PDAC organoids and matched PBMCs. Results After co-culturing PDAC organoids with PBMCs, we observed changes in $ CD4^{+} $, $ CD8^{+} $ and Treg cell populations. We observed favorable clinical outcome in patients whose PBMCs reacted to the co-culture with organoids. Conclusion This experimental model allows to investigate interactions between patient derived PDAC organoids and their PBMCs. This co-culture system could serve as a preclinical platform to guide personalized therapeutic strategies in the future. Pancreatic cancer (dpeaa)DE-He213 Organoids (dpeaa)DE-He213 Tumor-immune interaction (dpeaa)DE-He213 Pancreatic cancer organoids (dpeaa)DE-He213 Ma, T. aut Beirith, I. aut Koch, D. aut Hofmann, F. aut Heinrich, K. aut Aghamaliev, U. aut Sirtl, S. aut Westphalen, C. B. aut Nieß, H. aut Reichert, M. aut Angele, M. K. aut Regel, I. aut Bazhin, A. V. aut Werner, J. aut Ilmer, M. aut Renz, Bernhard W. aut Enthalten in Journal of cancer research and clinical oncology Berlin : Springer, 1904 149(2023), 14 vom: 20. Juli, Seite 13051-13064 (DE-627)253769515 (DE-600)1459285-X 1432-1335 nnns volume:149 year:2023 number:14 day:20 month:07 pages:13051-13064 https://dx.doi.org/10.1007/s00432-023-05100-7 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_165 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_267 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_711 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 149 2023 14 20 07 13051-13064
allfieldsSound	10.1007/s00432-023-05100-7 doi (DE-627)SPR053456416 (SPR)s00432-023-05100-7-e DE-627 ger DE-627 rakwb eng Knoblauch, M. verfasserin aut In-vitro model to mimic T cell subset change in human PDAC organoid co-culture 2023 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s) 2023 Purpose Immunotherapies have largely failed as treatment options for pancreatic ductal adenocarcinoma (PDAC). In this field, clinical translational studies into personalized treatment are of fundamental importance. In our study, we model tumor-cell immune-cell interactions in a co-culture of primary human PDAC organoids and matched peripheral blood mononuclear cells (PBMCs). Methods Using flow cytometry, we evaluated changes in T cell subtypes upon co-culture of patient-derived PDAC organoids and matched PBMCs. Results After co-culturing PDAC organoids with PBMCs, we observed changes in $ CD4^{+} $, $ CD8^{+} $ and Treg cell populations. We observed favorable clinical outcome in patients whose PBMCs reacted to the co-culture with organoids. Conclusion This experimental model allows to investigate interactions between patient derived PDAC organoids and their PBMCs. This co-culture system could serve as a preclinical platform to guide personalized therapeutic strategies in the future. Pancreatic cancer (dpeaa)DE-He213 Organoids (dpeaa)DE-He213 Tumor-immune interaction (dpeaa)DE-He213 Pancreatic cancer organoids (dpeaa)DE-He213 Ma, T. aut Beirith, I. aut Koch, D. aut Hofmann, F. aut Heinrich, K. aut Aghamaliev, U. aut Sirtl, S. aut Westphalen, C. B. aut Nieß, H. aut Reichert, M. aut Angele, M. K. aut Regel, I. aut Bazhin, A. V. aut Werner, J. aut Ilmer, M. aut Renz, Bernhard W. aut Enthalten in Journal of cancer research and clinical oncology Berlin : Springer, 1904 149(2023), 14 vom: 20. Juli, Seite 13051-13064 (DE-627)253769515 (DE-600)1459285-X 1432-1335 nnns volume:149 year:2023 number:14 day:20 month:07 pages:13051-13064 https://dx.doi.org/10.1007/s00432-023-05100-7 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_165 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_267 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_711 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 149 2023 14 20 07 13051-13064
language	English
source	Enthalten in Journal of cancer research and clinical oncology 149(2023), 14 vom: 20. Juli, Seite 13051-13064 volume:149 year:2023 number:14 day:20 month:07 pages:13051-13064
sourceStr	Enthalten in Journal of cancer research and clinical oncology 149(2023), 14 vom: 20. Juli, Seite 13051-13064 volume:149 year:2023 number:14 day:20 month:07 pages:13051-13064
format_phy_str_mv	Article
institution	findex.gbv.de
topic_facet	Pancreatic cancer Organoids Tumor-immune interaction Pancreatic cancer organoids
isfreeaccess_bool	true
container_title	Journal of cancer research and clinical oncology
authorswithroles_txt_mv	Knoblauch, M. @@aut@@ Ma, T. @@aut@@ Beirith, I. @@aut@@ Koch, D. @@aut@@ Hofmann, F. @@aut@@ Heinrich, K. @@aut@@ Aghamaliev, U. @@aut@@ Sirtl, S. @@aut@@ Westphalen, C. B. @@aut@@ Nieß, H. @@aut@@ Reichert, M. @@aut@@ Angele, M. K. @@aut@@ Regel, I. @@aut@@ Bazhin, A. V. @@aut@@ Werner, J. @@aut@@ Ilmer, M. @@aut@@ Renz, Bernhard W. @@aut@@
publishDateDaySort_date	2023-07-20T00:00:00Z
hierarchy_top_id	253769515
id	SPR053456416
language_de	englisch
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author	Knoblauch, M.
spellingShingle	Knoblauch, M. misc Pancreatic cancer misc Organoids misc Tumor-immune interaction misc Pancreatic cancer organoids In-vitro model to mimic T cell subset change in human PDAC organoid co-culture
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topic_title	In-vitro model to mimic T cell subset change in human PDAC organoid co-culture Pancreatic cancer (dpeaa)DE-He213 Organoids (dpeaa)DE-He213 Tumor-immune interaction (dpeaa)DE-He213 Pancreatic cancer organoids (dpeaa)DE-He213
topic	misc Pancreatic cancer misc Organoids misc Tumor-immune interaction misc Pancreatic cancer organoids
topic_unstemmed	misc Pancreatic cancer misc Organoids misc Tumor-immune interaction misc Pancreatic cancer organoids
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title	In-vitro model to mimic T cell subset change in human PDAC organoid co-culture
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title_full	In-vitro model to mimic T cell subset change in human PDAC organoid co-culture
author_sort	Knoblauch, M.
journal	Journal of cancer research and clinical oncology
journalStr	Journal of cancer research and clinical oncology
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author_browse	Knoblauch, M. Ma, T. Beirith, I. Koch, D. Hofmann, F. Heinrich, K. Aghamaliev, U. Sirtl, S. Westphalen, C. B. Nieß, H. Reichert, M. Angele, M. K. Regel, I. Bazhin, A. V. Werner, J. Ilmer, M. Renz, Bernhard W.
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title_sort	in-vitro model to mimic t cell subset change in human pdac organoid co-culture
title_auth	In-vitro model to mimic T cell subset change in human PDAC organoid co-culture
abstract	Purpose Immunotherapies have largely failed as treatment options for pancreatic ductal adenocarcinoma (PDAC). In this field, clinical translational studies into personalized treatment are of fundamental importance. In our study, we model tumor-cell immune-cell interactions in a co-culture of primary human PDAC organoids and matched peripheral blood mononuclear cells (PBMCs). Methods Using flow cytometry, we evaluated changes in T cell subtypes upon co-culture of patient-derived PDAC organoids and matched PBMCs. Results After co-culturing PDAC organoids with PBMCs, we observed changes in $ CD4^{+} $, $ CD8^{+} $ and Treg cell populations. We observed favorable clinical outcome in patients whose PBMCs reacted to the co-culture with organoids. Conclusion This experimental model allows to investigate interactions between patient derived PDAC organoids and their PBMCs. This co-culture system could serve as a preclinical platform to guide personalized therapeutic strategies in the future. © The Author(s) 2023
abstractGer	Purpose Immunotherapies have largely failed as treatment options for pancreatic ductal adenocarcinoma (PDAC). In this field, clinical translational studies into personalized treatment are of fundamental importance. In our study, we model tumor-cell immune-cell interactions in a co-culture of primary human PDAC organoids and matched peripheral blood mononuclear cells (PBMCs). Methods Using flow cytometry, we evaluated changes in T cell subtypes upon co-culture of patient-derived PDAC organoids and matched PBMCs. Results After co-culturing PDAC organoids with PBMCs, we observed changes in $ CD4^{+} $, $ CD8^{+} $ and Treg cell populations. We observed favorable clinical outcome in patients whose PBMCs reacted to the co-culture with organoids. Conclusion This experimental model allows to investigate interactions between patient derived PDAC organoids and their PBMCs. This co-culture system could serve as a preclinical platform to guide personalized therapeutic strategies in the future. © The Author(s) 2023
abstract_unstemmed	Purpose Immunotherapies have largely failed as treatment options for pancreatic ductal adenocarcinoma (PDAC). In this field, clinical translational studies into personalized treatment are of fundamental importance. In our study, we model tumor-cell immune-cell interactions in a co-culture of primary human PDAC organoids and matched peripheral blood mononuclear cells (PBMCs). Methods Using flow cytometry, we evaluated changes in T cell subtypes upon co-culture of patient-derived PDAC organoids and matched PBMCs. Results After co-culturing PDAC organoids with PBMCs, we observed changes in $ CD4^{+} $, $ CD8^{+} $ and Treg cell populations. We observed favorable clinical outcome in patients whose PBMCs reacted to the co-culture with organoids. Conclusion This experimental model allows to investigate interactions between patient derived PDAC organoids and their PBMCs. This co-culture system could serve as a preclinical platform to guide personalized therapeutic strategies in the future. © The Author(s) 2023
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title_short	In-vitro model to mimic T cell subset change in human PDAC organoid co-culture
url	https://dx.doi.org/10.1007/s00432-023-05100-7
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author2	Ma, T. Beirith, I. Koch, D. Hofmann, F. Heinrich, K. Aghamaliev, U. Sirtl, S. Westphalen, C. B. Nieß, H. Reichert, M. Angele, M. K. Regel, I. Bazhin, A. V. Werner, J. Ilmer, M. Renz, Bernhard W.
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up_date	2024-07-03T19:39:43.258Z
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In this field, clinical translational studies into personalized treatment are of fundamental importance. In our study, we model tumor-cell immune-cell interactions in a co-culture of primary human PDAC organoids and matched peripheral blood mononuclear cells (PBMCs). Methods Using flow cytometry, we evaluated changes in T cell subtypes upon co-culture of patient-derived PDAC organoids and matched PBMCs. Results After co-culturing PDAC organoids with PBMCs, we observed changes in $ CD4^{+} $, $ CD8^{+} $ and Treg cell populations. We observed favorable clinical outcome in patients whose PBMCs reacted to the co-culture with organoids. Conclusion This experimental model allows to investigate interactions between patient derived PDAC organoids and their PBMCs. 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In-vitro model to mimic T cell subset change in human PDAC organoid co-culture

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