Activity of propolis from Mexico on the proliferation and virulence factors of Candida albicans

Background This research evaluated the anti-Candida albicans effect of Mexican propolis from Chihuahua. Chemical composition of the ethanolic extract of propolis was determined by GC-MS, HPLC-DAD, and HPLC-MS. The presence of anthraquinone, aromatic acid, fatty acids, flavonoids, and carbohydrates w...
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Autor*in:	Rodriguez-Canales, Mario [verfasserIn] Medina-Romero, Yoli Mariana Rodriguez-Monroy, Marco Aurelio Nava-Solis, Uriel Bolaños-Cruz, Sandra Isabel Mendoza-Romero, Maria Jimena Campos, Jorge E. Hernandez-Hernandez, Ana Bertha Chirino, Yolanda I. Cruz-Sanchez, Tonatiuh Garcia-Tovar, Carlos Gerardo Canales-Martinez, Maria Margarita

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2023

Schlagwörter:	Natural antifungal agents Anti-opportunistic infection Natural products targets

Anmerkung:	© The Author(s) 2023

Übergeordnetes Werk:	Enthalten in: BMC microbiology - London : BioMed Central, 2001, 23(2023), 1 vom: 04. Nov.
Übergeordnetes Werk:	volume:23 ; year:2023 ; number:1 ; day:04 ; month:11

Links:	Volltext

DOI / URN:	10.1186/s12866-023-03064-9

Katalog-ID:	SPR05362176X

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520			\|a Background This research evaluated the anti-Candida albicans effect of Mexican propolis from Chihuahua. Chemical composition of the ethanolic extract of propolis was determined by GC-MS, HPLC-DAD, and HPLC-MS. The presence of anthraquinone, aromatic acid, fatty acids, flavonoids, and carbohydrates was revealed. Results The anti-Candida activity of propolis was determined. The inhibitions halos were between 10.0 to 11.8 mm; 25% minimum inhibitory concentration (0.5 mg/ml) was fungistatic, and 50% minimum inhibitory concentration (1.0 mg/ml) was fungicidal. The effect of propolis on the capability of C. albicans to change its morphology was evaluated. 25% minimum inhibitory concentration inhibited to 50% of germ tube formation. Staining with calcofluor-white and propidium iodide was performed, showing that the propolis affected the integrity of the cell membrane. INT1 gene expression was evaluated by qRT-PCR. Propolis significantly inhibited the expression of the INT1 gene encodes an adhesin (Int1p). Chihuahua propolis extract inhibited the proliferation of Candida albicans, the development of the germ tube, and the synthesis of adhesin INT1. Conclusions Given the properties demonstrated for Chihuahua propolis, we propose that it is a candidate to be considered as an ideal antifungal agent to help treat this infection since it would not have the toxic effects of conventional antifungals.
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allfields	10.1186/s12866-023-03064-9 doi (DE-627)SPR05362176X (SPR)s12866-023-03064-9-e DE-627 ger DE-627 rakwb eng Rodriguez-Canales, Mario verfasserin aut Activity of propolis from Mexico on the proliferation and virulence factors of Candida albicans 2023 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s) 2023 Background This research evaluated the anti-Candida albicans effect of Mexican propolis from Chihuahua. Chemical composition of the ethanolic extract of propolis was determined by GC-MS, HPLC-DAD, and HPLC-MS. The presence of anthraquinone, aromatic acid, fatty acids, flavonoids, and carbohydrates was revealed. Results The anti-Candida activity of propolis was determined. The inhibitions halos were between 10.0 to 11.8 mm; 25% minimum inhibitory concentration (0.5 mg/ml) was fungistatic, and 50% minimum inhibitory concentration (1.0 mg/ml) was fungicidal. The effect of propolis on the capability of C. albicans to change its morphology was evaluated. 25% minimum inhibitory concentration inhibited to 50% of germ tube formation. Staining with calcofluor-white and propidium iodide was performed, showing that the propolis affected the integrity of the cell membrane. INT1 gene expression was evaluated by qRT-PCR. Propolis significantly inhibited the expression of the INT1 gene encodes an adhesin (Int1p). Chihuahua propolis extract inhibited the proliferation of Candida albicans, the development of the germ tube, and the synthesis of adhesin INT1. Conclusions Given the properties demonstrated for Chihuahua propolis, we propose that it is a candidate to be considered as an ideal antifungal agent to help treat this infection since it would not have the toxic effects of conventional antifungals. Natural antifungal agents (dpeaa)DE-He213 Anti-opportunistic infection (dpeaa)DE-He213 Natural products targets (dpeaa)DE-He213 Medina-Romero, Yoli Mariana aut Rodriguez-Monroy, Marco Aurelio aut Nava-Solis, Uriel aut Bolaños-Cruz, Sandra Isabel aut Mendoza-Romero, Maria Jimena aut Campos, Jorge E. aut Hernandez-Hernandez, Ana Bertha aut Chirino, Yolanda I. aut Cruz-Sanchez, Tonatiuh aut Garcia-Tovar, Carlos Gerardo aut Canales-Martinez, Maria Margarita aut Enthalten in BMC microbiology London : BioMed Central, 2001 23(2023), 1 vom: 04. Nov. (DE-627)326644997 (DE-600)2041505-9 1471-2180 nnns volume:23 year:2023 number:1 day:04 month:11 https://dx.doi.org/10.1186/s12866-023-03064-9 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 23 2023 1 04 11
spelling	10.1186/s12866-023-03064-9 doi (DE-627)SPR05362176X (SPR)s12866-023-03064-9-e DE-627 ger DE-627 rakwb eng Rodriguez-Canales, Mario verfasserin aut Activity of propolis from Mexico on the proliferation and virulence factors of Candida albicans 2023 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s) 2023 Background This research evaluated the anti-Candida albicans effect of Mexican propolis from Chihuahua. Chemical composition of the ethanolic extract of propolis was determined by GC-MS, HPLC-DAD, and HPLC-MS. The presence of anthraquinone, aromatic acid, fatty acids, flavonoids, and carbohydrates was revealed. Results The anti-Candida activity of propolis was determined. The inhibitions halos were between 10.0 to 11.8 mm; 25% minimum inhibitory concentration (0.5 mg/ml) was fungistatic, and 50% minimum inhibitory concentration (1.0 mg/ml) was fungicidal. The effect of propolis on the capability of C. albicans to change its morphology was evaluated. 25% minimum inhibitory concentration inhibited to 50% of germ tube formation. Staining with calcofluor-white and propidium iodide was performed, showing that the propolis affected the integrity of the cell membrane. INT1 gene expression was evaluated by qRT-PCR. Propolis significantly inhibited the expression of the INT1 gene encodes an adhesin (Int1p). Chihuahua propolis extract inhibited the proliferation of Candida albicans, the development of the germ tube, and the synthesis of adhesin INT1. Conclusions Given the properties demonstrated for Chihuahua propolis, we propose that it is a candidate to be considered as an ideal antifungal agent to help treat this infection since it would not have the toxic effects of conventional antifungals. Natural antifungal agents (dpeaa)DE-He213 Anti-opportunistic infection (dpeaa)DE-He213 Natural products targets (dpeaa)DE-He213 Medina-Romero, Yoli Mariana aut Rodriguez-Monroy, Marco Aurelio aut Nava-Solis, Uriel aut Bolaños-Cruz, Sandra Isabel aut Mendoza-Romero, Maria Jimena aut Campos, Jorge E. aut Hernandez-Hernandez, Ana Bertha aut Chirino, Yolanda I. aut Cruz-Sanchez, Tonatiuh aut Garcia-Tovar, Carlos Gerardo aut Canales-Martinez, Maria Margarita aut Enthalten in BMC microbiology London : BioMed Central, 2001 23(2023), 1 vom: 04. Nov. (DE-627)326644997 (DE-600)2041505-9 1471-2180 nnns volume:23 year:2023 number:1 day:04 month:11 https://dx.doi.org/10.1186/s12866-023-03064-9 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 23 2023 1 04 11
allfields_unstemmed	10.1186/s12866-023-03064-9 doi (DE-627)SPR05362176X (SPR)s12866-023-03064-9-e DE-627 ger DE-627 rakwb eng Rodriguez-Canales, Mario verfasserin aut Activity of propolis from Mexico on the proliferation and virulence factors of Candida albicans 2023 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s) 2023 Background This research evaluated the anti-Candida albicans effect of Mexican propolis from Chihuahua. Chemical composition of the ethanolic extract of propolis was determined by GC-MS, HPLC-DAD, and HPLC-MS. The presence of anthraquinone, aromatic acid, fatty acids, flavonoids, and carbohydrates was revealed. Results The anti-Candida activity of propolis was determined. The inhibitions halos were between 10.0 to 11.8 mm; 25% minimum inhibitory concentration (0.5 mg/ml) was fungistatic, and 50% minimum inhibitory concentration (1.0 mg/ml) was fungicidal. The effect of propolis on the capability of C. albicans to change its morphology was evaluated. 25% minimum inhibitory concentration inhibited to 50% of germ tube formation. Staining with calcofluor-white and propidium iodide was performed, showing that the propolis affected the integrity of the cell membrane. INT1 gene expression was evaluated by qRT-PCR. Propolis significantly inhibited the expression of the INT1 gene encodes an adhesin (Int1p). Chihuahua propolis extract inhibited the proliferation of Candida albicans, the development of the germ tube, and the synthesis of adhesin INT1. Conclusions Given the properties demonstrated for Chihuahua propolis, we propose that it is a candidate to be considered as an ideal antifungal agent to help treat this infection since it would not have the toxic effects of conventional antifungals. Natural antifungal agents (dpeaa)DE-He213 Anti-opportunistic infection (dpeaa)DE-He213 Natural products targets (dpeaa)DE-He213 Medina-Romero, Yoli Mariana aut Rodriguez-Monroy, Marco Aurelio aut Nava-Solis, Uriel aut Bolaños-Cruz, Sandra Isabel aut Mendoza-Romero, Maria Jimena aut Campos, Jorge E. aut Hernandez-Hernandez, Ana Bertha aut Chirino, Yolanda I. aut Cruz-Sanchez, Tonatiuh aut Garcia-Tovar, Carlos Gerardo aut Canales-Martinez, Maria Margarita aut Enthalten in BMC microbiology London : BioMed Central, 2001 23(2023), 1 vom: 04. Nov. (DE-627)326644997 (DE-600)2041505-9 1471-2180 nnns volume:23 year:2023 number:1 day:04 month:11 https://dx.doi.org/10.1186/s12866-023-03064-9 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 23 2023 1 04 11
allfieldsGer	10.1186/s12866-023-03064-9 doi (DE-627)SPR05362176X (SPR)s12866-023-03064-9-e DE-627 ger DE-627 rakwb eng Rodriguez-Canales, Mario verfasserin aut Activity of propolis from Mexico on the proliferation and virulence factors of Candida albicans 2023 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s) 2023 Background This research evaluated the anti-Candida albicans effect of Mexican propolis from Chihuahua. Chemical composition of the ethanolic extract of propolis was determined by GC-MS, HPLC-DAD, and HPLC-MS. The presence of anthraquinone, aromatic acid, fatty acids, flavonoids, and carbohydrates was revealed. Results The anti-Candida activity of propolis was determined. The inhibitions halos were between 10.0 to 11.8 mm; 25% minimum inhibitory concentration (0.5 mg/ml) was fungistatic, and 50% minimum inhibitory concentration (1.0 mg/ml) was fungicidal. The effect of propolis on the capability of C. albicans to change its morphology was evaluated. 25% minimum inhibitory concentration inhibited to 50% of germ tube formation. Staining with calcofluor-white and propidium iodide was performed, showing that the propolis affected the integrity of the cell membrane. INT1 gene expression was evaluated by qRT-PCR. Propolis significantly inhibited the expression of the INT1 gene encodes an adhesin (Int1p). Chihuahua propolis extract inhibited the proliferation of Candida albicans, the development of the germ tube, and the synthesis of adhesin INT1. Conclusions Given the properties demonstrated for Chihuahua propolis, we propose that it is a candidate to be considered as an ideal antifungal agent to help treat this infection since it would not have the toxic effects of conventional antifungals. Natural antifungal agents (dpeaa)DE-He213 Anti-opportunistic infection (dpeaa)DE-He213 Natural products targets (dpeaa)DE-He213 Medina-Romero, Yoli Mariana aut Rodriguez-Monroy, Marco Aurelio aut Nava-Solis, Uriel aut Bolaños-Cruz, Sandra Isabel aut Mendoza-Romero, Maria Jimena aut Campos, Jorge E. aut Hernandez-Hernandez, Ana Bertha aut Chirino, Yolanda I. aut Cruz-Sanchez, Tonatiuh aut Garcia-Tovar, Carlos Gerardo aut Canales-Martinez, Maria Margarita aut Enthalten in BMC microbiology London : BioMed Central, 2001 23(2023), 1 vom: 04. Nov. (DE-627)326644997 (DE-600)2041505-9 1471-2180 nnns volume:23 year:2023 number:1 day:04 month:11 https://dx.doi.org/10.1186/s12866-023-03064-9 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 23 2023 1 04 11
allfieldsSound	10.1186/s12866-023-03064-9 doi (DE-627)SPR05362176X (SPR)s12866-023-03064-9-e DE-627 ger DE-627 rakwb eng Rodriguez-Canales, Mario verfasserin aut Activity of propolis from Mexico on the proliferation and virulence factors of Candida albicans 2023 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s) 2023 Background This research evaluated the anti-Candida albicans effect of Mexican propolis from Chihuahua. Chemical composition of the ethanolic extract of propolis was determined by GC-MS, HPLC-DAD, and HPLC-MS. The presence of anthraquinone, aromatic acid, fatty acids, flavonoids, and carbohydrates was revealed. Results The anti-Candida activity of propolis was determined. The inhibitions halos were between 10.0 to 11.8 mm; 25% minimum inhibitory concentration (0.5 mg/ml) was fungistatic, and 50% minimum inhibitory concentration (1.0 mg/ml) was fungicidal. The effect of propolis on the capability of C. albicans to change its morphology was evaluated. 25% minimum inhibitory concentration inhibited to 50% of germ tube formation. Staining with calcofluor-white and propidium iodide was performed, showing that the propolis affected the integrity of the cell membrane. INT1 gene expression was evaluated by qRT-PCR. Propolis significantly inhibited the expression of the INT1 gene encodes an adhesin (Int1p). Chihuahua propolis extract inhibited the proliferation of Candida albicans, the development of the germ tube, and the synthesis of adhesin INT1. Conclusions Given the properties demonstrated for Chihuahua propolis, we propose that it is a candidate to be considered as an ideal antifungal agent to help treat this infection since it would not have the toxic effects of conventional antifungals. Natural antifungal agents (dpeaa)DE-He213 Anti-opportunistic infection (dpeaa)DE-He213 Natural products targets (dpeaa)DE-He213 Medina-Romero, Yoli Mariana aut Rodriguez-Monroy, Marco Aurelio aut Nava-Solis, Uriel aut Bolaños-Cruz, Sandra Isabel aut Mendoza-Romero, Maria Jimena aut Campos, Jorge E. aut Hernandez-Hernandez, Ana Bertha aut Chirino, Yolanda I. aut Cruz-Sanchez, Tonatiuh aut Garcia-Tovar, Carlos Gerardo aut Canales-Martinez, Maria Margarita aut Enthalten in BMC microbiology London : BioMed Central, 2001 23(2023), 1 vom: 04. Nov. (DE-627)326644997 (DE-600)2041505-9 1471-2180 nnns volume:23 year:2023 number:1 day:04 month:11 https://dx.doi.org/10.1186/s12866-023-03064-9 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 23 2023 1 04 11
language	English
source	Enthalten in BMC microbiology 23(2023), 1 vom: 04. Nov. volume:23 year:2023 number:1 day:04 month:11
sourceStr	Enthalten in BMC microbiology 23(2023), 1 vom: 04. Nov. volume:23 year:2023 number:1 day:04 month:11
format_phy_str_mv	Article
institution	findex.gbv.de
topic_facet	Natural antifungal agents Anti-opportunistic infection Natural products targets
isfreeaccess_bool	true
container_title	BMC microbiology
authorswithroles_txt_mv	Rodriguez-Canales, Mario @@aut@@ Medina-Romero, Yoli Mariana @@aut@@ Rodriguez-Monroy, Marco Aurelio @@aut@@ Nava-Solis, Uriel @@aut@@ Bolaños-Cruz, Sandra Isabel @@aut@@ Mendoza-Romero, Maria Jimena @@aut@@ Campos, Jorge E. @@aut@@ Hernandez-Hernandez, Ana Bertha @@aut@@ Chirino, Yolanda I. @@aut@@ Cruz-Sanchez, Tonatiuh @@aut@@ Garcia-Tovar, Carlos Gerardo @@aut@@ Canales-Martinez, Maria Margarita @@aut@@
publishDateDaySort_date	2023-11-04T00:00:00Z
hierarchy_top_id	326644997
id	SPR05362176X
language_de	englisch
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author	Rodriguez-Canales, Mario
spellingShingle	Rodriguez-Canales, Mario misc Natural antifungal agents misc Anti-opportunistic infection misc Natural products targets Activity of propolis from Mexico on the proliferation and virulence factors of Candida albicans
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topic_title	Activity of propolis from Mexico on the proliferation and virulence factors of Candida albicans Natural antifungal agents (dpeaa)DE-He213 Anti-opportunistic infection (dpeaa)DE-He213 Natural products targets (dpeaa)DE-He213
topic	misc Natural antifungal agents misc Anti-opportunistic infection misc Natural products targets
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title	Activity of propolis from Mexico on the proliferation and virulence factors of Candida albicans
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title_full	Activity of propolis from Mexico on the proliferation and virulence factors of Candida albicans
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author_browse	Rodriguez-Canales, Mario Medina-Romero, Yoli Mariana Rodriguez-Monroy, Marco Aurelio Nava-Solis, Uriel Bolaños-Cruz, Sandra Isabel Mendoza-Romero, Maria Jimena Campos, Jorge E. Hernandez-Hernandez, Ana Bertha Chirino, Yolanda I. Cruz-Sanchez, Tonatiuh Garcia-Tovar, Carlos Gerardo Canales-Martinez, Maria Margarita
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author-letter	Rodriguez-Canales, Mario
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title_sort	activity of propolis from mexico on the proliferation and virulence factors of candida albicans
title_auth	Activity of propolis from Mexico on the proliferation and virulence factors of Candida albicans
abstract	Background This research evaluated the anti-Candida albicans effect of Mexican propolis from Chihuahua. Chemical composition of the ethanolic extract of propolis was determined by GC-MS, HPLC-DAD, and HPLC-MS. The presence of anthraquinone, aromatic acid, fatty acids, flavonoids, and carbohydrates was revealed. Results The anti-Candida activity of propolis was determined. The inhibitions halos were between 10.0 to 11.8 mm; 25% minimum inhibitory concentration (0.5 mg/ml) was fungistatic, and 50% minimum inhibitory concentration (1.0 mg/ml) was fungicidal. The effect of propolis on the capability of C. albicans to change its morphology was evaluated. 25% minimum inhibitory concentration inhibited to 50% of germ tube formation. Staining with calcofluor-white and propidium iodide was performed, showing that the propolis affected the integrity of the cell membrane. INT1 gene expression was evaluated by qRT-PCR. Propolis significantly inhibited the expression of the INT1 gene encodes an adhesin (Int1p). Chihuahua propolis extract inhibited the proliferation of Candida albicans, the development of the germ tube, and the synthesis of adhesin INT1. Conclusions Given the properties demonstrated for Chihuahua propolis, we propose that it is a candidate to be considered as an ideal antifungal agent to help treat this infection since it would not have the toxic effects of conventional antifungals. © The Author(s) 2023
abstractGer	Background This research evaluated the anti-Candida albicans effect of Mexican propolis from Chihuahua. Chemical composition of the ethanolic extract of propolis was determined by GC-MS, HPLC-DAD, and HPLC-MS. The presence of anthraquinone, aromatic acid, fatty acids, flavonoids, and carbohydrates was revealed. Results The anti-Candida activity of propolis was determined. The inhibitions halos were between 10.0 to 11.8 mm; 25% minimum inhibitory concentration (0.5 mg/ml) was fungistatic, and 50% minimum inhibitory concentration (1.0 mg/ml) was fungicidal. The effect of propolis on the capability of C. albicans to change its morphology was evaluated. 25% minimum inhibitory concentration inhibited to 50% of germ tube formation. Staining with calcofluor-white and propidium iodide was performed, showing that the propolis affected the integrity of the cell membrane. INT1 gene expression was evaluated by qRT-PCR. Propolis significantly inhibited the expression of the INT1 gene encodes an adhesin (Int1p). Chihuahua propolis extract inhibited the proliferation of Candida albicans, the development of the germ tube, and the synthesis of adhesin INT1. Conclusions Given the properties demonstrated for Chihuahua propolis, we propose that it is a candidate to be considered as an ideal antifungal agent to help treat this infection since it would not have the toxic effects of conventional antifungals. © The Author(s) 2023
abstract_unstemmed	Background This research evaluated the anti-Candida albicans effect of Mexican propolis from Chihuahua. Chemical composition of the ethanolic extract of propolis was determined by GC-MS, HPLC-DAD, and HPLC-MS. The presence of anthraquinone, aromatic acid, fatty acids, flavonoids, and carbohydrates was revealed. Results The anti-Candida activity of propolis was determined. The inhibitions halos were between 10.0 to 11.8 mm; 25% minimum inhibitory concentration (0.5 mg/ml) was fungistatic, and 50% minimum inhibitory concentration (1.0 mg/ml) was fungicidal. The effect of propolis on the capability of C. albicans to change its morphology was evaluated. 25% minimum inhibitory concentration inhibited to 50% of germ tube formation. Staining with calcofluor-white and propidium iodide was performed, showing that the propolis affected the integrity of the cell membrane. INT1 gene expression was evaluated by qRT-PCR. Propolis significantly inhibited the expression of the INT1 gene encodes an adhesin (Int1p). Chihuahua propolis extract inhibited the proliferation of Candida albicans, the development of the germ tube, and the synthesis of adhesin INT1. Conclusions Given the properties demonstrated for Chihuahua propolis, we propose that it is a candidate to be considered as an ideal antifungal agent to help treat this infection since it would not have the toxic effects of conventional antifungals. © The Author(s) 2023
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title_short	Activity of propolis from Mexico on the proliferation and virulence factors of Candida albicans
url	https://dx.doi.org/10.1186/s12866-023-03064-9
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author2	Medina-Romero, Yoli Mariana Rodriguez-Monroy, Marco Aurelio Nava-Solis, Uriel Bolaños-Cruz, Sandra Isabel Mendoza-Romero, Maria Jimena Campos, Jorge E. Hernandez-Hernandez, Ana Bertha Chirino, Yolanda I. Cruz-Sanchez, Tonatiuh Garcia-Tovar, Carlos Gerardo Canales-Martinez, Maria Margarita
author2Str	Medina-Romero, Yoli Mariana Rodriguez-Monroy, Marco Aurelio Nava-Solis, Uriel Bolaños-Cruz, Sandra Isabel Mendoza-Romero, Maria Jimena Campos, Jorge E. Hernandez-Hernandez, Ana Bertha Chirino, Yolanda I. Cruz-Sanchez, Tonatiuh Garcia-Tovar, Carlos Gerardo Canales-Martinez, Maria Margarita
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up_date	2024-07-03T20:51:17.491Z
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Activity of propolis from Mexico on the proliferation and virulence factors of Candida albicans

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