Changes in Fungal Community and Gene Expression of Specific Ligninolytic Enzymes in Response to Wood Decay
Abstract Microbial communities, particularly Basidiomycota fungi, play crucial roles in ecosystem functioning, including wood decay and the recycling of carbon and nutrients. However, the relationship between fungal diversity and wood decomposition remains poorly understood. This study investigated...
Ausführliche Beschreibung
Autor*in: |
Lee, Min [verfasserIn] |
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E-Artikel |
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Sprache: |
Englisch |
Erschienen: |
2023 |
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Schlagwörter: |
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Anmerkung: |
© The Korean Society for Biotechnology and Bioengineering and Springer 2023 |
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Übergeordnetes Werk: |
Enthalten in: Biotechnology and bioprocess engineering - Seoul : Society, 1996, 28(2023), 5 vom: Okt., Seite 826-834 |
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Übergeordnetes Werk: |
volume:28 ; year:2023 ; number:5 ; month:10 ; pages:826-834 |
Links: |
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DOI / URN: |
10.1007/s12257-023-0113-5 |
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Katalog-ID: |
SPR053681444 |
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520 | |a Abstract Microbial communities, particularly Basidiomycota fungi, play crucial roles in ecosystem functioning, including wood decay and the recycling of carbon and nutrients. However, the relationship between fungal diversity and wood decomposition remains poorly understood. This study investigated the effect of fungal community, gene expression, and activity of specific ligninolytic enzymes on wood decay in pine, cedar, and alkaline copper quaternary (ACQ)-treated pines for 30 months. The fungal community and gene expression levels were characterized using DNA sequencing and RT–qPCR, respectively. The Azure-B assay and phenol red test were conducted to determine enzyme activity. The results showed that non-wood-decaying fungi dominated early in the decay process, whereas true wood-decaying fungi, such as Trametes sp., dominated later. Visual decay ratings and modulus of elasticity data implied that pine deteriorated more than cedar and that ACQ treatment helped prevent deterioration of pine. The gene expression levels of lignin peroxidase (LiP), manganese peroxidase (MnP), and laccase (Lcc) produced by the predominant fungus, T. elegans, varied depending on the wood type. LiP expression was the highest in ACQ-treated pines after 10 months of treatment. MnP expression was higher in both decay-resistant woods (cedar- and ACQ-treated pines) than in untreated pines. No Lcc expression was detected. Moreover, the specific activity of these enzymes was generally higher in untreated pine than in the decay-resistant wood. This study contributes to the understanding of the role of fungal communities in wood decay and the impact of preservation treatments on the gene expression and activity of wood decay enzymes. | ||
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10.1007/s12257-023-0113-5 doi (DE-627)SPR053681444 (SPR)s12257-023-0113-5-e DE-627 ger DE-627 rakwb eng Lee, Min verfasserin aut Changes in Fungal Community and Gene Expression of Specific Ligninolytic Enzymes in Response to Wood Decay 2023 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Korean Society for Biotechnology and Bioengineering and Springer 2023 Abstract Microbial communities, particularly Basidiomycota fungi, play crucial roles in ecosystem functioning, including wood decay and the recycling of carbon and nutrients. However, the relationship between fungal diversity and wood decomposition remains poorly understood. This study investigated the effect of fungal community, gene expression, and activity of specific ligninolytic enzymes on wood decay in pine, cedar, and alkaline copper quaternary (ACQ)-treated pines for 30 months. The fungal community and gene expression levels were characterized using DNA sequencing and RT–qPCR, respectively. The Azure-B assay and phenol red test were conducted to determine enzyme activity. The results showed that non-wood-decaying fungi dominated early in the decay process, whereas true wood-decaying fungi, such as Trametes sp., dominated later. Visual decay ratings and modulus of elasticity data implied that pine deteriorated more than cedar and that ACQ treatment helped prevent deterioration of pine. The gene expression levels of lignin peroxidase (LiP), manganese peroxidase (MnP), and laccase (Lcc) produced by the predominant fungus, T. elegans, varied depending on the wood type. LiP expression was the highest in ACQ-treated pines after 10 months of treatment. MnP expression was higher in both decay-resistant woods (cedar- and ACQ-treated pines) than in untreated pines. No Lcc expression was detected. Moreover, the specific activity of these enzymes was generally higher in untreated pine than in the decay-resistant wood. This study contributes to the understanding of the role of fungal communities in wood decay and the impact of preservation treatments on the gene expression and activity of wood decay enzymes. alkaline copper quaternary (dpeaa)DE-He213 cedar (dpeaa)DE-He213 ligninolytic enzyme (dpeaa)DE-He213 pine (dpeaa)DE-He213 wood decay (dpeaa)DE-He213 Rahmat, Endang aut Prewitt, Lynn aut Gang, Roggers aut Ban, Yeongjun aut Kang, Chang Ho aut Kang, Youngmin aut Enthalten in Biotechnology and bioprocess engineering Seoul : Society, 1996 28(2023), 5 vom: Okt., Seite 826-834 (DE-627)373321821 (DE-600)2125481-3 1976-3816 nnns volume:28 year:2023 number:5 month:10 pages:826-834 https://dx.doi.org/10.1007/s12257-023-0113-5 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 28 2023 5 10 826-834 |
spelling |
10.1007/s12257-023-0113-5 doi (DE-627)SPR053681444 (SPR)s12257-023-0113-5-e DE-627 ger DE-627 rakwb eng Lee, Min verfasserin aut Changes in Fungal Community and Gene Expression of Specific Ligninolytic Enzymes in Response to Wood Decay 2023 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Korean Society for Biotechnology and Bioengineering and Springer 2023 Abstract Microbial communities, particularly Basidiomycota fungi, play crucial roles in ecosystem functioning, including wood decay and the recycling of carbon and nutrients. However, the relationship between fungal diversity and wood decomposition remains poorly understood. This study investigated the effect of fungal community, gene expression, and activity of specific ligninolytic enzymes on wood decay in pine, cedar, and alkaline copper quaternary (ACQ)-treated pines for 30 months. The fungal community and gene expression levels were characterized using DNA sequencing and RT–qPCR, respectively. The Azure-B assay and phenol red test were conducted to determine enzyme activity. The results showed that non-wood-decaying fungi dominated early in the decay process, whereas true wood-decaying fungi, such as Trametes sp., dominated later. Visual decay ratings and modulus of elasticity data implied that pine deteriorated more than cedar and that ACQ treatment helped prevent deterioration of pine. The gene expression levels of lignin peroxidase (LiP), manganese peroxidase (MnP), and laccase (Lcc) produced by the predominant fungus, T. elegans, varied depending on the wood type. LiP expression was the highest in ACQ-treated pines after 10 months of treatment. MnP expression was higher in both decay-resistant woods (cedar- and ACQ-treated pines) than in untreated pines. No Lcc expression was detected. Moreover, the specific activity of these enzymes was generally higher in untreated pine than in the decay-resistant wood. This study contributes to the understanding of the role of fungal communities in wood decay and the impact of preservation treatments on the gene expression and activity of wood decay enzymes. alkaline copper quaternary (dpeaa)DE-He213 cedar (dpeaa)DE-He213 ligninolytic enzyme (dpeaa)DE-He213 pine (dpeaa)DE-He213 wood decay (dpeaa)DE-He213 Rahmat, Endang aut Prewitt, Lynn aut Gang, Roggers aut Ban, Yeongjun aut Kang, Chang Ho aut Kang, Youngmin aut Enthalten in Biotechnology and bioprocess engineering Seoul : Society, 1996 28(2023), 5 vom: Okt., Seite 826-834 (DE-627)373321821 (DE-600)2125481-3 1976-3816 nnns volume:28 year:2023 number:5 month:10 pages:826-834 https://dx.doi.org/10.1007/s12257-023-0113-5 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 28 2023 5 10 826-834 |
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10.1007/s12257-023-0113-5 doi (DE-627)SPR053681444 (SPR)s12257-023-0113-5-e DE-627 ger DE-627 rakwb eng Lee, Min verfasserin aut Changes in Fungal Community and Gene Expression of Specific Ligninolytic Enzymes in Response to Wood Decay 2023 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Korean Society for Biotechnology and Bioengineering and Springer 2023 Abstract Microbial communities, particularly Basidiomycota fungi, play crucial roles in ecosystem functioning, including wood decay and the recycling of carbon and nutrients. However, the relationship between fungal diversity and wood decomposition remains poorly understood. This study investigated the effect of fungal community, gene expression, and activity of specific ligninolytic enzymes on wood decay in pine, cedar, and alkaline copper quaternary (ACQ)-treated pines for 30 months. The fungal community and gene expression levels were characterized using DNA sequencing and RT–qPCR, respectively. The Azure-B assay and phenol red test were conducted to determine enzyme activity. The results showed that non-wood-decaying fungi dominated early in the decay process, whereas true wood-decaying fungi, such as Trametes sp., dominated later. Visual decay ratings and modulus of elasticity data implied that pine deteriorated more than cedar and that ACQ treatment helped prevent deterioration of pine. The gene expression levels of lignin peroxidase (LiP), manganese peroxidase (MnP), and laccase (Lcc) produced by the predominant fungus, T. elegans, varied depending on the wood type. LiP expression was the highest in ACQ-treated pines after 10 months of treatment. MnP expression was higher in both decay-resistant woods (cedar- and ACQ-treated pines) than in untreated pines. No Lcc expression was detected. Moreover, the specific activity of these enzymes was generally higher in untreated pine than in the decay-resistant wood. This study contributes to the understanding of the role of fungal communities in wood decay and the impact of preservation treatments on the gene expression and activity of wood decay enzymes. alkaline copper quaternary (dpeaa)DE-He213 cedar (dpeaa)DE-He213 ligninolytic enzyme (dpeaa)DE-He213 pine (dpeaa)DE-He213 wood decay (dpeaa)DE-He213 Rahmat, Endang aut Prewitt, Lynn aut Gang, Roggers aut Ban, Yeongjun aut Kang, Chang Ho aut Kang, Youngmin aut Enthalten in Biotechnology and bioprocess engineering Seoul : Society, 1996 28(2023), 5 vom: Okt., Seite 826-834 (DE-627)373321821 (DE-600)2125481-3 1976-3816 nnns volume:28 year:2023 number:5 month:10 pages:826-834 https://dx.doi.org/10.1007/s12257-023-0113-5 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 28 2023 5 10 826-834 |
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10.1007/s12257-023-0113-5 doi (DE-627)SPR053681444 (SPR)s12257-023-0113-5-e DE-627 ger DE-627 rakwb eng Lee, Min verfasserin aut Changes in Fungal Community and Gene Expression of Specific Ligninolytic Enzymes in Response to Wood Decay 2023 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Korean Society for Biotechnology and Bioengineering and Springer 2023 Abstract Microbial communities, particularly Basidiomycota fungi, play crucial roles in ecosystem functioning, including wood decay and the recycling of carbon and nutrients. However, the relationship between fungal diversity and wood decomposition remains poorly understood. This study investigated the effect of fungal community, gene expression, and activity of specific ligninolytic enzymes on wood decay in pine, cedar, and alkaline copper quaternary (ACQ)-treated pines for 30 months. The fungal community and gene expression levels were characterized using DNA sequencing and RT–qPCR, respectively. The Azure-B assay and phenol red test were conducted to determine enzyme activity. The results showed that non-wood-decaying fungi dominated early in the decay process, whereas true wood-decaying fungi, such as Trametes sp., dominated later. Visual decay ratings and modulus of elasticity data implied that pine deteriorated more than cedar and that ACQ treatment helped prevent deterioration of pine. The gene expression levels of lignin peroxidase (LiP), manganese peroxidase (MnP), and laccase (Lcc) produced by the predominant fungus, T. elegans, varied depending on the wood type. LiP expression was the highest in ACQ-treated pines after 10 months of treatment. MnP expression was higher in both decay-resistant woods (cedar- and ACQ-treated pines) than in untreated pines. No Lcc expression was detected. Moreover, the specific activity of these enzymes was generally higher in untreated pine than in the decay-resistant wood. This study contributes to the understanding of the role of fungal communities in wood decay and the impact of preservation treatments on the gene expression and activity of wood decay enzymes. alkaline copper quaternary (dpeaa)DE-He213 cedar (dpeaa)DE-He213 ligninolytic enzyme (dpeaa)DE-He213 pine (dpeaa)DE-He213 wood decay (dpeaa)DE-He213 Rahmat, Endang aut Prewitt, Lynn aut Gang, Roggers aut Ban, Yeongjun aut Kang, Chang Ho aut Kang, Youngmin aut Enthalten in Biotechnology and bioprocess engineering Seoul : Society, 1996 28(2023), 5 vom: Okt., Seite 826-834 (DE-627)373321821 (DE-600)2125481-3 1976-3816 nnns volume:28 year:2023 number:5 month:10 pages:826-834 https://dx.doi.org/10.1007/s12257-023-0113-5 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 28 2023 5 10 826-834 |
allfieldsSound |
10.1007/s12257-023-0113-5 doi (DE-627)SPR053681444 (SPR)s12257-023-0113-5-e DE-627 ger DE-627 rakwb eng Lee, Min verfasserin aut Changes in Fungal Community and Gene Expression of Specific Ligninolytic Enzymes in Response to Wood Decay 2023 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Korean Society for Biotechnology and Bioengineering and Springer 2023 Abstract Microbial communities, particularly Basidiomycota fungi, play crucial roles in ecosystem functioning, including wood decay and the recycling of carbon and nutrients. However, the relationship between fungal diversity and wood decomposition remains poorly understood. This study investigated the effect of fungal community, gene expression, and activity of specific ligninolytic enzymes on wood decay in pine, cedar, and alkaline copper quaternary (ACQ)-treated pines for 30 months. The fungal community and gene expression levels were characterized using DNA sequencing and RT–qPCR, respectively. The Azure-B assay and phenol red test were conducted to determine enzyme activity. The results showed that non-wood-decaying fungi dominated early in the decay process, whereas true wood-decaying fungi, such as Trametes sp., dominated later. Visual decay ratings and modulus of elasticity data implied that pine deteriorated more than cedar and that ACQ treatment helped prevent deterioration of pine. The gene expression levels of lignin peroxidase (LiP), manganese peroxidase (MnP), and laccase (Lcc) produced by the predominant fungus, T. elegans, varied depending on the wood type. LiP expression was the highest in ACQ-treated pines after 10 months of treatment. MnP expression was higher in both decay-resistant woods (cedar- and ACQ-treated pines) than in untreated pines. No Lcc expression was detected. Moreover, the specific activity of these enzymes was generally higher in untreated pine than in the decay-resistant wood. This study contributes to the understanding of the role of fungal communities in wood decay and the impact of preservation treatments on the gene expression and activity of wood decay enzymes. alkaline copper quaternary (dpeaa)DE-He213 cedar (dpeaa)DE-He213 ligninolytic enzyme (dpeaa)DE-He213 pine (dpeaa)DE-He213 wood decay (dpeaa)DE-He213 Rahmat, Endang aut Prewitt, Lynn aut Gang, Roggers aut Ban, Yeongjun aut Kang, Chang Ho aut Kang, Youngmin aut Enthalten in Biotechnology and bioprocess engineering Seoul : Society, 1996 28(2023), 5 vom: Okt., Seite 826-834 (DE-627)373321821 (DE-600)2125481-3 1976-3816 nnns volume:28 year:2023 number:5 month:10 pages:826-834 https://dx.doi.org/10.1007/s12257-023-0113-5 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 28 2023 5 10 826-834 |
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Enthalten in Biotechnology and bioprocess engineering 28(2023), 5 vom: Okt., Seite 826-834 volume:28 year:2023 number:5 month:10 pages:826-834 |
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Lee, Min @@aut@@ Rahmat, Endang @@aut@@ Prewitt, Lynn @@aut@@ Gang, Roggers @@aut@@ Ban, Yeongjun @@aut@@ Kang, Chang Ho @@aut@@ Kang, Youngmin @@aut@@ |
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<?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>01000naa a22002652 4500</leader><controlfield tag="001">SPR053681444</controlfield><controlfield tag="003">DE-627</controlfield><controlfield tag="005">20231110064702.0</controlfield><controlfield tag="007">cr uuu---uuuuu</controlfield><controlfield tag="008">231110s2023 xx |||||o 00| ||eng c</controlfield><datafield tag="024" ind1="7" ind2=" "><subfield code="a">10.1007/s12257-023-0113-5</subfield><subfield code="2">doi</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-627)SPR053681444</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(SPR)s12257-023-0113-5-e</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-627</subfield><subfield code="b">ger</subfield><subfield code="c">DE-627</subfield><subfield code="e">rakwb</subfield></datafield><datafield tag="041" ind1=" " ind2=" "><subfield code="a">eng</subfield></datafield><datafield tag="100" ind1="1" ind2=" "><subfield code="a">Lee, Min</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">Changes in Fungal Community and Gene Expression of Specific Ligninolytic Enzymes in Response to Wood Decay</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="c">2023</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="a">Text</subfield><subfield code="b">txt</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="a">Computermedien</subfield><subfield code="b">c</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="a">Online-Ressource</subfield><subfield code="b">cr</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="500" ind1=" " ind2=" "><subfield code="a">© The Korean Society for Biotechnology and Bioengineering and Springer 2023</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a">Abstract Microbial communities, particularly Basidiomycota fungi, play crucial roles in ecosystem functioning, including wood decay and the recycling of carbon and nutrients. However, the relationship between fungal diversity and wood decomposition remains poorly understood. This study investigated the effect of fungal community, gene expression, and activity of specific ligninolytic enzymes on wood decay in pine, cedar, and alkaline copper quaternary (ACQ)-treated pines for 30 months. The fungal community and gene expression levels were characterized using DNA sequencing and RT–qPCR, respectively. The Azure-B assay and phenol red test were conducted to determine enzyme activity. The results showed that non-wood-decaying fungi dominated early in the decay process, whereas true wood-decaying fungi, such as Trametes sp., dominated later. Visual decay ratings and modulus of elasticity data implied that pine deteriorated more than cedar and that ACQ treatment helped prevent deterioration of pine. The gene expression levels of lignin peroxidase (LiP), manganese peroxidase (MnP), and laccase (Lcc) produced by the predominant fungus, T. elegans, varied depending on the wood type. LiP expression was the highest in ACQ-treated pines after 10 months of treatment. MnP expression was higher in both decay-resistant woods (cedar- and ACQ-treated pines) than in untreated pines. No Lcc expression was detected. Moreover, the specific activity of these enzymes was generally higher in untreated pine than in the decay-resistant wood. 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Lee, Min |
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Lee, Min misc alkaline copper quaternary misc cedar misc ligninolytic enzyme misc pine misc wood decay Changes in Fungal Community and Gene Expression of Specific Ligninolytic Enzymes in Response to Wood Decay |
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Changes in Fungal Community and Gene Expression of Specific Ligninolytic Enzymes in Response to Wood Decay alkaline copper quaternary (dpeaa)DE-He213 cedar (dpeaa)DE-He213 ligninolytic enzyme (dpeaa)DE-He213 pine (dpeaa)DE-He213 wood decay (dpeaa)DE-He213 |
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changes in fungal community and gene expression of specific ligninolytic enzymes in response to wood decay |
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Changes in Fungal Community and Gene Expression of Specific Ligninolytic Enzymes in Response to Wood Decay |
abstract |
Abstract Microbial communities, particularly Basidiomycota fungi, play crucial roles in ecosystem functioning, including wood decay and the recycling of carbon and nutrients. However, the relationship between fungal diversity and wood decomposition remains poorly understood. This study investigated the effect of fungal community, gene expression, and activity of specific ligninolytic enzymes on wood decay in pine, cedar, and alkaline copper quaternary (ACQ)-treated pines for 30 months. The fungal community and gene expression levels were characterized using DNA sequencing and RT–qPCR, respectively. The Azure-B assay and phenol red test were conducted to determine enzyme activity. The results showed that non-wood-decaying fungi dominated early in the decay process, whereas true wood-decaying fungi, such as Trametes sp., dominated later. Visual decay ratings and modulus of elasticity data implied that pine deteriorated more than cedar and that ACQ treatment helped prevent deterioration of pine. The gene expression levels of lignin peroxidase (LiP), manganese peroxidase (MnP), and laccase (Lcc) produced by the predominant fungus, T. elegans, varied depending on the wood type. LiP expression was the highest in ACQ-treated pines after 10 months of treatment. MnP expression was higher in both decay-resistant woods (cedar- and ACQ-treated pines) than in untreated pines. No Lcc expression was detected. Moreover, the specific activity of these enzymes was generally higher in untreated pine than in the decay-resistant wood. This study contributes to the understanding of the role of fungal communities in wood decay and the impact of preservation treatments on the gene expression and activity of wood decay enzymes. © The Korean Society for Biotechnology and Bioengineering and Springer 2023 |
abstractGer |
Abstract Microbial communities, particularly Basidiomycota fungi, play crucial roles in ecosystem functioning, including wood decay and the recycling of carbon and nutrients. However, the relationship between fungal diversity and wood decomposition remains poorly understood. This study investigated the effect of fungal community, gene expression, and activity of specific ligninolytic enzymes on wood decay in pine, cedar, and alkaline copper quaternary (ACQ)-treated pines for 30 months. The fungal community and gene expression levels were characterized using DNA sequencing and RT–qPCR, respectively. The Azure-B assay and phenol red test were conducted to determine enzyme activity. The results showed that non-wood-decaying fungi dominated early in the decay process, whereas true wood-decaying fungi, such as Trametes sp., dominated later. Visual decay ratings and modulus of elasticity data implied that pine deteriorated more than cedar and that ACQ treatment helped prevent deterioration of pine. The gene expression levels of lignin peroxidase (LiP), manganese peroxidase (MnP), and laccase (Lcc) produced by the predominant fungus, T. elegans, varied depending on the wood type. LiP expression was the highest in ACQ-treated pines after 10 months of treatment. MnP expression was higher in both decay-resistant woods (cedar- and ACQ-treated pines) than in untreated pines. No Lcc expression was detected. Moreover, the specific activity of these enzymes was generally higher in untreated pine than in the decay-resistant wood. This study contributes to the understanding of the role of fungal communities in wood decay and the impact of preservation treatments on the gene expression and activity of wood decay enzymes. © The Korean Society for Biotechnology and Bioengineering and Springer 2023 |
abstract_unstemmed |
Abstract Microbial communities, particularly Basidiomycota fungi, play crucial roles in ecosystem functioning, including wood decay and the recycling of carbon and nutrients. However, the relationship between fungal diversity and wood decomposition remains poorly understood. This study investigated the effect of fungal community, gene expression, and activity of specific ligninolytic enzymes on wood decay in pine, cedar, and alkaline copper quaternary (ACQ)-treated pines for 30 months. The fungal community and gene expression levels were characterized using DNA sequencing and RT–qPCR, respectively. The Azure-B assay and phenol red test were conducted to determine enzyme activity. The results showed that non-wood-decaying fungi dominated early in the decay process, whereas true wood-decaying fungi, such as Trametes sp., dominated later. Visual decay ratings and modulus of elasticity data implied that pine deteriorated more than cedar and that ACQ treatment helped prevent deterioration of pine. The gene expression levels of lignin peroxidase (LiP), manganese peroxidase (MnP), and laccase (Lcc) produced by the predominant fungus, T. elegans, varied depending on the wood type. LiP expression was the highest in ACQ-treated pines after 10 months of treatment. MnP expression was higher in both decay-resistant woods (cedar- and ACQ-treated pines) than in untreated pines. No Lcc expression was detected. Moreover, the specific activity of these enzymes was generally higher in untreated pine than in the decay-resistant wood. This study contributes to the understanding of the role of fungal communities in wood decay and the impact of preservation treatments on the gene expression and activity of wood decay enzymes. © The Korean Society for Biotechnology and Bioengineering and Springer 2023 |
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5 |
title_short |
Changes in Fungal Community and Gene Expression of Specific Ligninolytic Enzymes in Response to Wood Decay |
url |
https://dx.doi.org/10.1007/s12257-023-0113-5 |
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Rahmat, Endang Prewitt, Lynn Gang, Roggers Ban, Yeongjun Kang, Chang Ho Kang, Youngmin |
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up_date |
2024-07-03T21:16:36.274Z |
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score |
7.398576 |