Regulation of cultured coral endosymbiont photophysiology by alternate heat stress protocols
Abstract Unlocking how coral endosymbionts (Symbiodiniaceae) regulate the susceptibility of corals to thermal stress-induced bleaching has been the focus of experimental heat stress studies on laboratory-maintained isolates for over 30 years. Multiple protocols to induce heat stress have been used,...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
Bartels, Natasha [verfasserIn] |
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| Format: |
E-Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
2023 |
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| Schlagwörter: |
Light-induced fluorescence transient fast repetition rate fluorometry |
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| Anmerkung: |
© The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature 2023 |
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| Übergeordnetes Werk: |
Enthalten in: Marine biology - Berlin : Springer, 1967, 171(2023), 1 vom: 14. Dez. |
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| Übergeordnetes Werk: |
volume:171 ; year:2023 ; number:1 ; day:14 ; month:12 |
| Links: |
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| DOI / URN: |
10.1007/s00227-023-04360-8 |
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| Katalog-ID: |
SPR054081033 |
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| 520 | |a Abstract Unlocking how coral endosymbionts (Symbiodiniaceae) regulate the susceptibility of corals to thermal stress-induced bleaching has been the focus of experimental heat stress studies on laboratory-maintained isolates for over 30 years. Multiple protocols to induce heat stress have been used, but the extent to which data can be reconciled across protocols remains unknown. We therefore first analysed past published heat stress studies on Symbiodiniaceae (Jan 1980–Jan 2023) to identify the range of protocols (ramping rate derived from time taken to reach the target heat stress temperatures from the initial ambient temperature, time retained under the target heat stress temperature, etc.) commonly used for experimentation. Temperature differences between ambient and maximum experimental temperatures spanned from 3 °C to 17 °C (mean = 6.8 °C), and only 41% of all studies reported a ramping rate, ranging from instantaneous to 0.02 °C/h (mean = 0.62 °C/h). Experiment length at the treatment temperature varied from < 24 h to > 2 months. We next experimentally quantified if the rate of heating impacted the observed physiological outcome for three Symbiodiniaceae taxa with differences in heat tolerance—Durusdinium trenchii, Breviolum psygmophilum and Breviolum minutum. Five experimental treatments were performed with different temperature ramping rates from 26 °C to 32 °C: 1 °C/1 h, 1 °C/12 h, 1 °C/1 d, 1 °C/2 d and 1 °C/3 d, spanning relatively acute to chronic. Changes in physiology were quantified daily as the maximum photochemical efficiency (Fv/Fm). Heating commonly induced decreases in Fv/Fm, and the change between the start and end of experiment (∆Fv/Fm) linearly increased with ramping rates. Our data suggests that slower ramping exacerbates thermal stress, though with varying magnitude across taxa as B. minutum, and D. trenchii proved less resilient than B. pysgmophilum. We discuss how these outcomes potentially impact the capacity to integrate biological responses observed from past experiments and highlights the need for more standardised experimental protocols. | ||
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10.1007/s00227-023-04360-8 doi (DE-627)SPR054081033 (SPR)s00227-023-04360-8-e DE-627 ger DE-627 rakwb eng Bartels, Natasha verfasserin (orcid)0000-0002-0904-8866 aut Regulation of cultured coral endosymbiont photophysiology by alternate heat stress protocols 2023 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature 2023 Abstract Unlocking how coral endosymbionts (Symbiodiniaceae) regulate the susceptibility of corals to thermal stress-induced bleaching has been the focus of experimental heat stress studies on laboratory-maintained isolates for over 30 years. Multiple protocols to induce heat stress have been used, but the extent to which data can be reconciled across protocols remains unknown. We therefore first analysed past published heat stress studies on Symbiodiniaceae (Jan 1980–Jan 2023) to identify the range of protocols (ramping rate derived from time taken to reach the target heat stress temperatures from the initial ambient temperature, time retained under the target heat stress temperature, etc.) commonly used for experimentation. Temperature differences between ambient and maximum experimental temperatures spanned from 3 °C to 17 °C (mean = 6.8 °C), and only 41% of all studies reported a ramping rate, ranging from instantaneous to 0.02 °C/h (mean = 0.62 °C/h). Experiment length at the treatment temperature varied from < 24 h to > 2 months. We next experimentally quantified if the rate of heating impacted the observed physiological outcome for three Symbiodiniaceae taxa with differences in heat tolerance—Durusdinium trenchii, Breviolum psygmophilum and Breviolum minutum. Five experimental treatments were performed with different temperature ramping rates from 26 °C to 32 °C: 1 °C/1 h, 1 °C/12 h, 1 °C/1 d, 1 °C/2 d and 1 °C/3 d, spanning relatively acute to chronic. Changes in physiology were quantified daily as the maximum photochemical efficiency (Fv/Fm). Heating commonly induced decreases in Fv/Fm, and the change between the start and end of experiment (∆Fv/Fm) linearly increased with ramping rates. Our data suggests that slower ramping exacerbates thermal stress, though with varying magnitude across taxa as B. minutum, and D. trenchii proved less resilient than B. pysgmophilum. We discuss how these outcomes potentially impact the capacity to integrate biological responses observed from past experiments and highlights the need for more standardised experimental protocols. Symbiodiniaceae (dpeaa)DE-He213 Heat stress (dpeaa)DE-He213 Thermal protocols (dpeaa)DE-He213 Light-induced fluorescence transient fast repetition rate fluorometry (dpeaa)DE-He213 Photosystem II (dpeaa)DE-He213 Photochemical efficiency (dpeaa)DE-He213 Camp, Emma F. (orcid)0000-0003-1962-1336 aut Dilernia, Nicole J. (orcid)0000-0002-0872-7402 aut Suggett, David J. (orcid)0000-0001-5326-2520 aut Enthalten in Marine biology Berlin : Springer, 1967 171(2023), 1 vom: 14. Dez. (DE-627)25377067X (DE-600)1459413-4 1432-1793 nnns volume:171 year:2023 number:1 day:14 month:12 https://dx.doi.org/10.1007/s00227-023-04360-8 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_267 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_381 GBV_ILN_602 GBV_ILN_636 GBV_ILN_647 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4277 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 171 2023 1 14 12 |
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10.1007/s00227-023-04360-8 doi (DE-627)SPR054081033 (SPR)s00227-023-04360-8-e DE-627 ger DE-627 rakwb eng Bartels, Natasha verfasserin (orcid)0000-0002-0904-8866 aut Regulation of cultured coral endosymbiont photophysiology by alternate heat stress protocols 2023 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature 2023 Abstract Unlocking how coral endosymbionts (Symbiodiniaceae) regulate the susceptibility of corals to thermal stress-induced bleaching has been the focus of experimental heat stress studies on laboratory-maintained isolates for over 30 years. Multiple protocols to induce heat stress have been used, but the extent to which data can be reconciled across protocols remains unknown. We therefore first analysed past published heat stress studies on Symbiodiniaceae (Jan 1980–Jan 2023) to identify the range of protocols (ramping rate derived from time taken to reach the target heat stress temperatures from the initial ambient temperature, time retained under the target heat stress temperature, etc.) commonly used for experimentation. Temperature differences between ambient and maximum experimental temperatures spanned from 3 °C to 17 °C (mean = 6.8 °C), and only 41% of all studies reported a ramping rate, ranging from instantaneous to 0.02 °C/h (mean = 0.62 °C/h). Experiment length at the treatment temperature varied from < 24 h to > 2 months. We next experimentally quantified if the rate of heating impacted the observed physiological outcome for three Symbiodiniaceae taxa with differences in heat tolerance—Durusdinium trenchii, Breviolum psygmophilum and Breviolum minutum. Five experimental treatments were performed with different temperature ramping rates from 26 °C to 32 °C: 1 °C/1 h, 1 °C/12 h, 1 °C/1 d, 1 °C/2 d and 1 °C/3 d, spanning relatively acute to chronic. Changes in physiology were quantified daily as the maximum photochemical efficiency (Fv/Fm). Heating commonly induced decreases in Fv/Fm, and the change between the start and end of experiment (∆Fv/Fm) linearly increased with ramping rates. Our data suggests that slower ramping exacerbates thermal stress, though with varying magnitude across taxa as B. minutum, and D. trenchii proved less resilient than B. pysgmophilum. We discuss how these outcomes potentially impact the capacity to integrate biological responses observed from past experiments and highlights the need for more standardised experimental protocols. Symbiodiniaceae (dpeaa)DE-He213 Heat stress (dpeaa)DE-He213 Thermal protocols (dpeaa)DE-He213 Light-induced fluorescence transient fast repetition rate fluorometry (dpeaa)DE-He213 Photosystem II (dpeaa)DE-He213 Photochemical efficiency (dpeaa)DE-He213 Camp, Emma F. (orcid)0000-0003-1962-1336 aut Dilernia, Nicole J. (orcid)0000-0002-0872-7402 aut Suggett, David J. (orcid)0000-0001-5326-2520 aut Enthalten in Marine biology Berlin : Springer, 1967 171(2023), 1 vom: 14. Dez. (DE-627)25377067X (DE-600)1459413-4 1432-1793 nnns volume:171 year:2023 number:1 day:14 month:12 https://dx.doi.org/10.1007/s00227-023-04360-8 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_267 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_381 GBV_ILN_602 GBV_ILN_636 GBV_ILN_647 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4277 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 171 2023 1 14 12 |
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10.1007/s00227-023-04360-8 doi (DE-627)SPR054081033 (SPR)s00227-023-04360-8-e DE-627 ger DE-627 rakwb eng Bartels, Natasha verfasserin (orcid)0000-0002-0904-8866 aut Regulation of cultured coral endosymbiont photophysiology by alternate heat stress protocols 2023 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature 2023 Abstract Unlocking how coral endosymbionts (Symbiodiniaceae) regulate the susceptibility of corals to thermal stress-induced bleaching has been the focus of experimental heat stress studies on laboratory-maintained isolates for over 30 years. Multiple protocols to induce heat stress have been used, but the extent to which data can be reconciled across protocols remains unknown. We therefore first analysed past published heat stress studies on Symbiodiniaceae (Jan 1980–Jan 2023) to identify the range of protocols (ramping rate derived from time taken to reach the target heat stress temperatures from the initial ambient temperature, time retained under the target heat stress temperature, etc.) commonly used for experimentation. Temperature differences between ambient and maximum experimental temperatures spanned from 3 °C to 17 °C (mean = 6.8 °C), and only 41% of all studies reported a ramping rate, ranging from instantaneous to 0.02 °C/h (mean = 0.62 °C/h). Experiment length at the treatment temperature varied from < 24 h to > 2 months. We next experimentally quantified if the rate of heating impacted the observed physiological outcome for three Symbiodiniaceae taxa with differences in heat tolerance—Durusdinium trenchii, Breviolum psygmophilum and Breviolum minutum. Five experimental treatments were performed with different temperature ramping rates from 26 °C to 32 °C: 1 °C/1 h, 1 °C/12 h, 1 °C/1 d, 1 °C/2 d and 1 °C/3 d, spanning relatively acute to chronic. Changes in physiology were quantified daily as the maximum photochemical efficiency (Fv/Fm). Heating commonly induced decreases in Fv/Fm, and the change between the start and end of experiment (∆Fv/Fm) linearly increased with ramping rates. Our data suggests that slower ramping exacerbates thermal stress, though with varying magnitude across taxa as B. minutum, and D. trenchii proved less resilient than B. pysgmophilum. We discuss how these outcomes potentially impact the capacity to integrate biological responses observed from past experiments and highlights the need for more standardised experimental protocols. Symbiodiniaceae (dpeaa)DE-He213 Heat stress (dpeaa)DE-He213 Thermal protocols (dpeaa)DE-He213 Light-induced fluorescence transient fast repetition rate fluorometry (dpeaa)DE-He213 Photosystem II (dpeaa)DE-He213 Photochemical efficiency (dpeaa)DE-He213 Camp, Emma F. (orcid)0000-0003-1962-1336 aut Dilernia, Nicole J. (orcid)0000-0002-0872-7402 aut Suggett, David J. (orcid)0000-0001-5326-2520 aut Enthalten in Marine biology Berlin : Springer, 1967 171(2023), 1 vom: 14. Dez. (DE-627)25377067X (DE-600)1459413-4 1432-1793 nnns volume:171 year:2023 number:1 day:14 month:12 https://dx.doi.org/10.1007/s00227-023-04360-8 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_267 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_381 GBV_ILN_602 GBV_ILN_636 GBV_ILN_647 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4277 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 171 2023 1 14 12 |
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10.1007/s00227-023-04360-8 doi (DE-627)SPR054081033 (SPR)s00227-023-04360-8-e DE-627 ger DE-627 rakwb eng Bartels, Natasha verfasserin (orcid)0000-0002-0904-8866 aut Regulation of cultured coral endosymbiont photophysiology by alternate heat stress protocols 2023 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature 2023 Abstract Unlocking how coral endosymbionts (Symbiodiniaceae) regulate the susceptibility of corals to thermal stress-induced bleaching has been the focus of experimental heat stress studies on laboratory-maintained isolates for over 30 years. Multiple protocols to induce heat stress have been used, but the extent to which data can be reconciled across protocols remains unknown. We therefore first analysed past published heat stress studies on Symbiodiniaceae (Jan 1980–Jan 2023) to identify the range of protocols (ramping rate derived from time taken to reach the target heat stress temperatures from the initial ambient temperature, time retained under the target heat stress temperature, etc.) commonly used for experimentation. Temperature differences between ambient and maximum experimental temperatures spanned from 3 °C to 17 °C (mean = 6.8 °C), and only 41% of all studies reported a ramping rate, ranging from instantaneous to 0.02 °C/h (mean = 0.62 °C/h). Experiment length at the treatment temperature varied from < 24 h to > 2 months. We next experimentally quantified if the rate of heating impacted the observed physiological outcome for three Symbiodiniaceae taxa with differences in heat tolerance—Durusdinium trenchii, Breviolum psygmophilum and Breviolum minutum. Five experimental treatments were performed with different temperature ramping rates from 26 °C to 32 °C: 1 °C/1 h, 1 °C/12 h, 1 °C/1 d, 1 °C/2 d and 1 °C/3 d, spanning relatively acute to chronic. Changes in physiology were quantified daily as the maximum photochemical efficiency (Fv/Fm). Heating commonly induced decreases in Fv/Fm, and the change between the start and end of experiment (∆Fv/Fm) linearly increased with ramping rates. Our data suggests that slower ramping exacerbates thermal stress, though with varying magnitude across taxa as B. minutum, and D. trenchii proved less resilient than B. pysgmophilum. We discuss how these outcomes potentially impact the capacity to integrate biological responses observed from past experiments and highlights the need for more standardised experimental protocols. Symbiodiniaceae (dpeaa)DE-He213 Heat stress (dpeaa)DE-He213 Thermal protocols (dpeaa)DE-He213 Light-induced fluorescence transient fast repetition rate fluorometry (dpeaa)DE-He213 Photosystem II (dpeaa)DE-He213 Photochemical efficiency (dpeaa)DE-He213 Camp, Emma F. (orcid)0000-0003-1962-1336 aut Dilernia, Nicole J. (orcid)0000-0002-0872-7402 aut Suggett, David J. (orcid)0000-0001-5326-2520 aut Enthalten in Marine biology Berlin : Springer, 1967 171(2023), 1 vom: 14. Dez. (DE-627)25377067X (DE-600)1459413-4 1432-1793 nnns volume:171 year:2023 number:1 day:14 month:12 https://dx.doi.org/10.1007/s00227-023-04360-8 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_267 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_381 GBV_ILN_602 GBV_ILN_636 GBV_ILN_647 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4277 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 171 2023 1 14 12 |
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10.1007/s00227-023-04360-8 doi (DE-627)SPR054081033 (SPR)s00227-023-04360-8-e DE-627 ger DE-627 rakwb eng Bartels, Natasha verfasserin (orcid)0000-0002-0904-8866 aut Regulation of cultured coral endosymbiont photophysiology by alternate heat stress protocols 2023 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature 2023 Abstract Unlocking how coral endosymbionts (Symbiodiniaceae) regulate the susceptibility of corals to thermal stress-induced bleaching has been the focus of experimental heat stress studies on laboratory-maintained isolates for over 30 years. Multiple protocols to induce heat stress have been used, but the extent to which data can be reconciled across protocols remains unknown. We therefore first analysed past published heat stress studies on Symbiodiniaceae (Jan 1980–Jan 2023) to identify the range of protocols (ramping rate derived from time taken to reach the target heat stress temperatures from the initial ambient temperature, time retained under the target heat stress temperature, etc.) commonly used for experimentation. Temperature differences between ambient and maximum experimental temperatures spanned from 3 °C to 17 °C (mean = 6.8 °C), and only 41% of all studies reported a ramping rate, ranging from instantaneous to 0.02 °C/h (mean = 0.62 °C/h). Experiment length at the treatment temperature varied from < 24 h to > 2 months. We next experimentally quantified if the rate of heating impacted the observed physiological outcome for three Symbiodiniaceae taxa with differences in heat tolerance—Durusdinium trenchii, Breviolum psygmophilum and Breviolum minutum. Five experimental treatments were performed with different temperature ramping rates from 26 °C to 32 °C: 1 °C/1 h, 1 °C/12 h, 1 °C/1 d, 1 °C/2 d and 1 °C/3 d, spanning relatively acute to chronic. Changes in physiology were quantified daily as the maximum photochemical efficiency (Fv/Fm). Heating commonly induced decreases in Fv/Fm, and the change between the start and end of experiment (∆Fv/Fm) linearly increased with ramping rates. Our data suggests that slower ramping exacerbates thermal stress, though with varying magnitude across taxa as B. minutum, and D. trenchii proved less resilient than B. pysgmophilum. We discuss how these outcomes potentially impact the capacity to integrate biological responses observed from past experiments and highlights the need for more standardised experimental protocols. Symbiodiniaceae (dpeaa)DE-He213 Heat stress (dpeaa)DE-He213 Thermal protocols (dpeaa)DE-He213 Light-induced fluorescence transient fast repetition rate fluorometry (dpeaa)DE-He213 Photosystem II (dpeaa)DE-He213 Photochemical efficiency (dpeaa)DE-He213 Camp, Emma F. (orcid)0000-0003-1962-1336 aut Dilernia, Nicole J. (orcid)0000-0002-0872-7402 aut Suggett, David J. (orcid)0000-0001-5326-2520 aut Enthalten in Marine biology Berlin : Springer, 1967 171(2023), 1 vom: 14. Dez. (DE-627)25377067X (DE-600)1459413-4 1432-1793 nnns volume:171 year:2023 number:1 day:14 month:12 https://dx.doi.org/10.1007/s00227-023-04360-8 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_267 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_381 GBV_ILN_602 GBV_ILN_636 GBV_ILN_647 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4277 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 171 2023 1 14 12 |
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Enthalten in Marine biology 171(2023), 1 vom: 14. Dez. volume:171 year:2023 number:1 day:14 month:12 |
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Multiple protocols to induce heat stress have been used, but the extent to which data can be reconciled across protocols remains unknown. We therefore first analysed past published heat stress studies on Symbiodiniaceae (Jan 1980–Jan 2023) to identify the range of protocols (ramping rate derived from time taken to reach the target heat stress temperatures from the initial ambient temperature, time retained under the target heat stress temperature, etc.) commonly used for experimentation. Temperature differences between ambient and maximum experimental temperatures spanned from 3 °C to 17 °C (mean = 6.8 °C), and only 41% of all studies reported a ramping rate, ranging from instantaneous to 0.02 °C/h (mean = 0.62 °C/h). Experiment length at the treatment temperature varied from < 24 h to > 2 months. 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Bartels, Natasha |
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Bartels, Natasha misc Symbiodiniaceae misc Heat stress misc Thermal protocols misc Light-induced fluorescence transient fast repetition rate fluorometry misc Photosystem II misc Photochemical efficiency Regulation of cultured coral endosymbiont photophysiology by alternate heat stress protocols |
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Regulation of cultured coral endosymbiont photophysiology by alternate heat stress protocols Symbiodiniaceae (dpeaa)DE-He213 Heat stress (dpeaa)DE-He213 Thermal protocols (dpeaa)DE-He213 Light-induced fluorescence transient fast repetition rate fluorometry (dpeaa)DE-He213 Photosystem II (dpeaa)DE-He213 Photochemical efficiency (dpeaa)DE-He213 |
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regulation of cultured coral endosymbiont photophysiology by alternate heat stress protocols |
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Regulation of cultured coral endosymbiont photophysiology by alternate heat stress protocols |
| abstract |
Abstract Unlocking how coral endosymbionts (Symbiodiniaceae) regulate the susceptibility of corals to thermal stress-induced bleaching has been the focus of experimental heat stress studies on laboratory-maintained isolates for over 30 years. Multiple protocols to induce heat stress have been used, but the extent to which data can be reconciled across protocols remains unknown. We therefore first analysed past published heat stress studies on Symbiodiniaceae (Jan 1980–Jan 2023) to identify the range of protocols (ramping rate derived from time taken to reach the target heat stress temperatures from the initial ambient temperature, time retained under the target heat stress temperature, etc.) commonly used for experimentation. Temperature differences between ambient and maximum experimental temperatures spanned from 3 °C to 17 °C (mean = 6.8 °C), and only 41% of all studies reported a ramping rate, ranging from instantaneous to 0.02 °C/h (mean = 0.62 °C/h). Experiment length at the treatment temperature varied from < 24 h to > 2 months. We next experimentally quantified if the rate of heating impacted the observed physiological outcome for three Symbiodiniaceae taxa with differences in heat tolerance—Durusdinium trenchii, Breviolum psygmophilum and Breviolum minutum. Five experimental treatments were performed with different temperature ramping rates from 26 °C to 32 °C: 1 °C/1 h, 1 °C/12 h, 1 °C/1 d, 1 °C/2 d and 1 °C/3 d, spanning relatively acute to chronic. Changes in physiology were quantified daily as the maximum photochemical efficiency (Fv/Fm). Heating commonly induced decreases in Fv/Fm, and the change between the start and end of experiment (∆Fv/Fm) linearly increased with ramping rates. Our data suggests that slower ramping exacerbates thermal stress, though with varying magnitude across taxa as B. minutum, and D. trenchii proved less resilient than B. pysgmophilum. We discuss how these outcomes potentially impact the capacity to integrate biological responses observed from past experiments and highlights the need for more standardised experimental protocols. © The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature 2023 |
| abstractGer |
Abstract Unlocking how coral endosymbionts (Symbiodiniaceae) regulate the susceptibility of corals to thermal stress-induced bleaching has been the focus of experimental heat stress studies on laboratory-maintained isolates for over 30 years. Multiple protocols to induce heat stress have been used, but the extent to which data can be reconciled across protocols remains unknown. We therefore first analysed past published heat stress studies on Symbiodiniaceae (Jan 1980–Jan 2023) to identify the range of protocols (ramping rate derived from time taken to reach the target heat stress temperatures from the initial ambient temperature, time retained under the target heat stress temperature, etc.) commonly used for experimentation. Temperature differences between ambient and maximum experimental temperatures spanned from 3 °C to 17 °C (mean = 6.8 °C), and only 41% of all studies reported a ramping rate, ranging from instantaneous to 0.02 °C/h (mean = 0.62 °C/h). Experiment length at the treatment temperature varied from < 24 h to > 2 months. We next experimentally quantified if the rate of heating impacted the observed physiological outcome for three Symbiodiniaceae taxa with differences in heat tolerance—Durusdinium trenchii, Breviolum psygmophilum and Breviolum minutum. Five experimental treatments were performed with different temperature ramping rates from 26 °C to 32 °C: 1 °C/1 h, 1 °C/12 h, 1 °C/1 d, 1 °C/2 d and 1 °C/3 d, spanning relatively acute to chronic. Changes in physiology were quantified daily as the maximum photochemical efficiency (Fv/Fm). Heating commonly induced decreases in Fv/Fm, and the change between the start and end of experiment (∆Fv/Fm) linearly increased with ramping rates. Our data suggests that slower ramping exacerbates thermal stress, though with varying magnitude across taxa as B. minutum, and D. trenchii proved less resilient than B. pysgmophilum. We discuss how these outcomes potentially impact the capacity to integrate biological responses observed from past experiments and highlights the need for more standardised experimental protocols. © The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature 2023 |
| abstract_unstemmed |
Abstract Unlocking how coral endosymbionts (Symbiodiniaceae) regulate the susceptibility of corals to thermal stress-induced bleaching has been the focus of experimental heat stress studies on laboratory-maintained isolates for over 30 years. Multiple protocols to induce heat stress have been used, but the extent to which data can be reconciled across protocols remains unknown. We therefore first analysed past published heat stress studies on Symbiodiniaceae (Jan 1980–Jan 2023) to identify the range of protocols (ramping rate derived from time taken to reach the target heat stress temperatures from the initial ambient temperature, time retained under the target heat stress temperature, etc.) commonly used for experimentation. Temperature differences between ambient and maximum experimental temperatures spanned from 3 °C to 17 °C (mean = 6.8 °C), and only 41% of all studies reported a ramping rate, ranging from instantaneous to 0.02 °C/h (mean = 0.62 °C/h). Experiment length at the treatment temperature varied from < 24 h to > 2 months. We next experimentally quantified if the rate of heating impacted the observed physiological outcome for three Symbiodiniaceae taxa with differences in heat tolerance—Durusdinium trenchii, Breviolum psygmophilum and Breviolum minutum. Five experimental treatments were performed with different temperature ramping rates from 26 °C to 32 °C: 1 °C/1 h, 1 °C/12 h, 1 °C/1 d, 1 °C/2 d and 1 °C/3 d, spanning relatively acute to chronic. Changes in physiology were quantified daily as the maximum photochemical efficiency (Fv/Fm). Heating commonly induced decreases in Fv/Fm, and the change between the start and end of experiment (∆Fv/Fm) linearly increased with ramping rates. Our data suggests that slower ramping exacerbates thermal stress, though with varying magnitude across taxa as B. minutum, and D. trenchii proved less resilient than B. pysgmophilum. We discuss how these outcomes potentially impact the capacity to integrate biological responses observed from past experiments and highlights the need for more standardised experimental protocols. © The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature 2023 |
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Regulation of cultured coral endosymbiont photophysiology by alternate heat stress protocols |
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Camp, Emma F. Dilernia, Nicole J. Suggett, David J. |
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| score |
7.401127 |