Test performance study to evaluate diagnostic tests for the detection and identification of Xylophilus ampelinus in vine material spiked with cells from X. ampelinus cultures
Abstract A test performance study (TPS) was conducted in 2020 to evaluate performance of serological and PCR-based tests for the detection of Xylophilus ampelinus in homogenised vine samples. In total, 11 labs participated, although there were fewer participants for the serological tests than the PC...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
Harrison, Catherine [verfasserIn] |
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| Format: |
E-Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
2023 |
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| Schlagwörter: |
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| Anmerkung: |
© Fera Science Ltd. 2023 |
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| Übergeordnetes Werk: |
Enthalten in: European journal of plant pathology - Dordrecht [u.a.] : Springer Science + Business Media B.V, 1895, 168(2023), 1 vom: 10. Okt., Seite 1-13 |
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| Übergeordnetes Werk: |
volume:168 ; year:2023 ; number:1 ; day:10 ; month:10 ; pages:1-13 |
| Links: |
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| DOI / URN: |
10.1007/s10658-023-02713-z |
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| Katalog-ID: |
SPR054223083 |
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| 100 | 1 | |a Harrison, Catherine |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Test performance study to evaluate diagnostic tests for the detection and identification of Xylophilus ampelinus in vine material spiked with cells from X. ampelinus cultures |
| 264 | 1 | |c 2023 | |
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| 520 | |a Abstract A test performance study (TPS) was conducted in 2020 to evaluate performance of serological and PCR-based tests for the detection of Xylophilus ampelinus in homogenised vine samples. In total, 11 labs participated, although there were fewer participants for the serological tests than the PCR-based tests. The panel of samples sent to participants included spiked samples containing $ 10^{4} $–$ 10^{8} $ cfu per ml (serological tests) or $ 10^{2} $–$ 10^{6} $ cfu per ml (PCR-based tests) as well as positive and negative controls. The five PCR-based tests were found to be fit for purpose, with similar performance across a range of metrics (analytical sensitivity, diagnostic sensitivity and specificity, and repeatability and reproducibility assessed in terms of accordance and concordance, respectively). Serological methods (two immunofluorescence tests and two ELISA tests) were found to be less sensitive with regard to both analytical and diagnostic sensitivity. Furthermore, the occurrence of false positives suggests that a positive IF result may not be conclusive when considered in isolation. One of the ELISA tests exhibited much lower analytical and diagnostic sensitivity than the other serological tests and would not be considered suitable for the purpose considered by this TPS. | ||
| 650 | 4 | |a PCR |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a Performance criteria |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a Serological tests |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a Test performance study |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a Validation |7 (dpeaa)DE-He213 | |
| 700 | 1 | |a Tomlinson, Jenny |4 aut | |
| 700 | 1 | |a Brittain, Ian |4 aut | |
| 700 | 1 | |a Laurenson, Lynn |4 aut | |
| 700 | 1 | |a van den Berg, Femke |0 (orcid)0000-0003-3885-1890 |4 aut | |
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10.1007/s10658-023-02713-z doi (DE-627)SPR054223083 (SPR)s10658-023-02713-z-e DE-627 ger DE-627 rakwb eng Harrison, Catherine verfasserin aut Test performance study to evaluate diagnostic tests for the detection and identification of Xylophilus ampelinus in vine material spiked with cells from X. ampelinus cultures 2023 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Fera Science Ltd. 2023 Abstract A test performance study (TPS) was conducted in 2020 to evaluate performance of serological and PCR-based tests for the detection of Xylophilus ampelinus in homogenised vine samples. In total, 11 labs participated, although there were fewer participants for the serological tests than the PCR-based tests. The panel of samples sent to participants included spiked samples containing $ 10^{4} $–$ 10^{8} $ cfu per ml (serological tests) or $ 10^{2} $–$ 10^{6} $ cfu per ml (PCR-based tests) as well as positive and negative controls. The five PCR-based tests were found to be fit for purpose, with similar performance across a range of metrics (analytical sensitivity, diagnostic sensitivity and specificity, and repeatability and reproducibility assessed in terms of accordance and concordance, respectively). Serological methods (two immunofluorescence tests and two ELISA tests) were found to be less sensitive with regard to both analytical and diagnostic sensitivity. Furthermore, the occurrence of false positives suggests that a positive IF result may not be conclusive when considered in isolation. One of the ELISA tests exhibited much lower analytical and diagnostic sensitivity than the other serological tests and would not be considered suitable for the purpose considered by this TPS. PCR (dpeaa)DE-He213 Performance criteria (dpeaa)DE-He213 Serological tests (dpeaa)DE-He213 Test performance study (dpeaa)DE-He213 Validation (dpeaa)DE-He213 Tomlinson, Jenny aut Brittain, Ian aut Laurenson, Lynn aut van den Berg, Femke (orcid)0000-0003-3885-1890 aut Enthalten in European journal of plant pathology Dordrecht [u.a.] : Springer Science + Business Media B.V, 1895 168(2023), 1 vom: 10. Okt., Seite 1-13 (DE-627)27042976X (DE-600)1477679-0 1573-8469 nnns volume:168 year:2023 number:1 day:10 month:10 pages:1-13 https://dx.doi.org/10.1007/s10658-023-02713-z kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 168 2023 1 10 10 1-13 |
| spelling |
10.1007/s10658-023-02713-z doi (DE-627)SPR054223083 (SPR)s10658-023-02713-z-e DE-627 ger DE-627 rakwb eng Harrison, Catherine verfasserin aut Test performance study to evaluate diagnostic tests for the detection and identification of Xylophilus ampelinus in vine material spiked with cells from X. ampelinus cultures 2023 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Fera Science Ltd. 2023 Abstract A test performance study (TPS) was conducted in 2020 to evaluate performance of serological and PCR-based tests for the detection of Xylophilus ampelinus in homogenised vine samples. In total, 11 labs participated, although there were fewer participants for the serological tests than the PCR-based tests. The panel of samples sent to participants included spiked samples containing $ 10^{4} $–$ 10^{8} $ cfu per ml (serological tests) or $ 10^{2} $–$ 10^{6} $ cfu per ml (PCR-based tests) as well as positive and negative controls. The five PCR-based tests were found to be fit for purpose, with similar performance across a range of metrics (analytical sensitivity, diagnostic sensitivity and specificity, and repeatability and reproducibility assessed in terms of accordance and concordance, respectively). Serological methods (two immunofluorescence tests and two ELISA tests) were found to be less sensitive with regard to both analytical and diagnostic sensitivity. Furthermore, the occurrence of false positives suggests that a positive IF result may not be conclusive when considered in isolation. One of the ELISA tests exhibited much lower analytical and diagnostic sensitivity than the other serological tests and would not be considered suitable for the purpose considered by this TPS. PCR (dpeaa)DE-He213 Performance criteria (dpeaa)DE-He213 Serological tests (dpeaa)DE-He213 Test performance study (dpeaa)DE-He213 Validation (dpeaa)DE-He213 Tomlinson, Jenny aut Brittain, Ian aut Laurenson, Lynn aut van den Berg, Femke (orcid)0000-0003-3885-1890 aut Enthalten in European journal of plant pathology Dordrecht [u.a.] : Springer Science + Business Media B.V, 1895 168(2023), 1 vom: 10. Okt., Seite 1-13 (DE-627)27042976X (DE-600)1477679-0 1573-8469 nnns volume:168 year:2023 number:1 day:10 month:10 pages:1-13 https://dx.doi.org/10.1007/s10658-023-02713-z kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 168 2023 1 10 10 1-13 |
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10.1007/s10658-023-02713-z doi (DE-627)SPR054223083 (SPR)s10658-023-02713-z-e DE-627 ger DE-627 rakwb eng Harrison, Catherine verfasserin aut Test performance study to evaluate diagnostic tests for the detection and identification of Xylophilus ampelinus in vine material spiked with cells from X. ampelinus cultures 2023 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Fera Science Ltd. 2023 Abstract A test performance study (TPS) was conducted in 2020 to evaluate performance of serological and PCR-based tests for the detection of Xylophilus ampelinus in homogenised vine samples. In total, 11 labs participated, although there were fewer participants for the serological tests than the PCR-based tests. The panel of samples sent to participants included spiked samples containing $ 10^{4} $–$ 10^{8} $ cfu per ml (serological tests) or $ 10^{2} $–$ 10^{6} $ cfu per ml (PCR-based tests) as well as positive and negative controls. The five PCR-based tests were found to be fit for purpose, with similar performance across a range of metrics (analytical sensitivity, diagnostic sensitivity and specificity, and repeatability and reproducibility assessed in terms of accordance and concordance, respectively). Serological methods (two immunofluorescence tests and two ELISA tests) were found to be less sensitive with regard to both analytical and diagnostic sensitivity. Furthermore, the occurrence of false positives suggests that a positive IF result may not be conclusive when considered in isolation. One of the ELISA tests exhibited much lower analytical and diagnostic sensitivity than the other serological tests and would not be considered suitable for the purpose considered by this TPS. PCR (dpeaa)DE-He213 Performance criteria (dpeaa)DE-He213 Serological tests (dpeaa)DE-He213 Test performance study (dpeaa)DE-He213 Validation (dpeaa)DE-He213 Tomlinson, Jenny aut Brittain, Ian aut Laurenson, Lynn aut van den Berg, Femke (orcid)0000-0003-3885-1890 aut Enthalten in European journal of plant pathology Dordrecht [u.a.] : Springer Science + Business Media B.V, 1895 168(2023), 1 vom: 10. Okt., Seite 1-13 (DE-627)27042976X (DE-600)1477679-0 1573-8469 nnns volume:168 year:2023 number:1 day:10 month:10 pages:1-13 https://dx.doi.org/10.1007/s10658-023-02713-z kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 168 2023 1 10 10 1-13 |
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10.1007/s10658-023-02713-z doi (DE-627)SPR054223083 (SPR)s10658-023-02713-z-e DE-627 ger DE-627 rakwb eng Harrison, Catherine verfasserin aut Test performance study to evaluate diagnostic tests for the detection and identification of Xylophilus ampelinus in vine material spiked with cells from X. ampelinus cultures 2023 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Fera Science Ltd. 2023 Abstract A test performance study (TPS) was conducted in 2020 to evaluate performance of serological and PCR-based tests for the detection of Xylophilus ampelinus in homogenised vine samples. In total, 11 labs participated, although there were fewer participants for the serological tests than the PCR-based tests. The panel of samples sent to participants included spiked samples containing $ 10^{4} $–$ 10^{8} $ cfu per ml (serological tests) or $ 10^{2} $–$ 10^{6} $ cfu per ml (PCR-based tests) as well as positive and negative controls. The five PCR-based tests were found to be fit for purpose, with similar performance across a range of metrics (analytical sensitivity, diagnostic sensitivity and specificity, and repeatability and reproducibility assessed in terms of accordance and concordance, respectively). Serological methods (two immunofluorescence tests and two ELISA tests) were found to be less sensitive with regard to both analytical and diagnostic sensitivity. Furthermore, the occurrence of false positives suggests that a positive IF result may not be conclusive when considered in isolation. One of the ELISA tests exhibited much lower analytical and diagnostic sensitivity than the other serological tests and would not be considered suitable for the purpose considered by this TPS. PCR (dpeaa)DE-He213 Performance criteria (dpeaa)DE-He213 Serological tests (dpeaa)DE-He213 Test performance study (dpeaa)DE-He213 Validation (dpeaa)DE-He213 Tomlinson, Jenny aut Brittain, Ian aut Laurenson, Lynn aut van den Berg, Femke (orcid)0000-0003-3885-1890 aut Enthalten in European journal of plant pathology Dordrecht [u.a.] : Springer Science + Business Media B.V, 1895 168(2023), 1 vom: 10. Okt., Seite 1-13 (DE-627)27042976X (DE-600)1477679-0 1573-8469 nnns volume:168 year:2023 number:1 day:10 month:10 pages:1-13 https://dx.doi.org/10.1007/s10658-023-02713-z kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 168 2023 1 10 10 1-13 |
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10.1007/s10658-023-02713-z doi (DE-627)SPR054223083 (SPR)s10658-023-02713-z-e DE-627 ger DE-627 rakwb eng Harrison, Catherine verfasserin aut Test performance study to evaluate diagnostic tests for the detection and identification of Xylophilus ampelinus in vine material spiked with cells from X. ampelinus cultures 2023 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © Fera Science Ltd. 2023 Abstract A test performance study (TPS) was conducted in 2020 to evaluate performance of serological and PCR-based tests for the detection of Xylophilus ampelinus in homogenised vine samples. In total, 11 labs participated, although there were fewer participants for the serological tests than the PCR-based tests. The panel of samples sent to participants included spiked samples containing $ 10^{4} $–$ 10^{8} $ cfu per ml (serological tests) or $ 10^{2} $–$ 10^{6} $ cfu per ml (PCR-based tests) as well as positive and negative controls. The five PCR-based tests were found to be fit for purpose, with similar performance across a range of metrics (analytical sensitivity, diagnostic sensitivity and specificity, and repeatability and reproducibility assessed in terms of accordance and concordance, respectively). Serological methods (two immunofluorescence tests and two ELISA tests) were found to be less sensitive with regard to both analytical and diagnostic sensitivity. Furthermore, the occurrence of false positives suggests that a positive IF result may not be conclusive when considered in isolation. One of the ELISA tests exhibited much lower analytical and diagnostic sensitivity than the other serological tests and would not be considered suitable for the purpose considered by this TPS. PCR (dpeaa)DE-He213 Performance criteria (dpeaa)DE-He213 Serological tests (dpeaa)DE-He213 Test performance study (dpeaa)DE-He213 Validation (dpeaa)DE-He213 Tomlinson, Jenny aut Brittain, Ian aut Laurenson, Lynn aut van den Berg, Femke (orcid)0000-0003-3885-1890 aut Enthalten in European journal of plant pathology Dordrecht [u.a.] : Springer Science + Business Media B.V, 1895 168(2023), 1 vom: 10. Okt., Seite 1-13 (DE-627)27042976X (DE-600)1477679-0 1573-8469 nnns volume:168 year:2023 number:1 day:10 month:10 pages:1-13 https://dx.doi.org/10.1007/s10658-023-02713-z kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 168 2023 1 10 10 1-13 |
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<?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>01000naa a22002652 4500</leader><controlfield tag="001">SPR054223083</controlfield><controlfield tag="003">DE-627</controlfield><controlfield tag="005">20240103064823.0</controlfield><controlfield tag="007">cr uuu---uuuuu</controlfield><controlfield tag="008">240103s2023 xx |||||o 00| ||eng c</controlfield><datafield tag="024" ind1="7" ind2=" "><subfield code="a">10.1007/s10658-023-02713-z</subfield><subfield code="2">doi</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-627)SPR054223083</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(SPR)s10658-023-02713-z-e</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-627</subfield><subfield code="b">ger</subfield><subfield code="c">DE-627</subfield><subfield code="e">rakwb</subfield></datafield><datafield tag="041" ind1=" " ind2=" "><subfield code="a">eng</subfield></datafield><datafield tag="100" ind1="1" ind2=" "><subfield code="a">Harrison, Catherine</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">Test performance study to evaluate diagnostic tests for the detection and identification of Xylophilus ampelinus in vine material spiked with cells from X. ampelinus cultures</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="c">2023</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="a">Text</subfield><subfield code="b">txt</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="a">Computermedien</subfield><subfield code="b">c</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="a">Online-Ressource</subfield><subfield code="b">cr</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="500" ind1=" " ind2=" "><subfield code="a">© Fera Science Ltd. 2023</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a">Abstract A test performance study (TPS) was conducted in 2020 to evaluate performance of serological and PCR-based tests for the detection of Xylophilus ampelinus in homogenised vine samples. In total, 11 labs participated, although there were fewer participants for the serological tests than the PCR-based tests. The panel of samples sent to participants included spiked samples containing $ 10^{4} $–$ 10^{8} $ cfu per ml (serological tests) or $ 10^{2} $–$ 10^{6} $ cfu per ml (PCR-based tests) as well as positive and negative controls. The five PCR-based tests were found to be fit for purpose, with similar performance across a range of metrics (analytical sensitivity, diagnostic sensitivity and specificity, and repeatability and reproducibility assessed in terms of accordance and concordance, respectively). Serological methods (two immunofluorescence tests and two ELISA tests) were found to be less sensitive with regard to both analytical and diagnostic sensitivity. Furthermore, the occurrence of false positives suggests that a positive IF result may not be conclusive when considered in isolation. 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Harrison, Catherine |
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Test performance study to evaluate diagnostic tests for the detection and identification of Xylophilus ampelinus in vine material spiked with cells from X. ampelinus cultures PCR (dpeaa)DE-He213 Performance criteria (dpeaa)DE-He213 Serological tests (dpeaa)DE-He213 Test performance study (dpeaa)DE-He213 Validation (dpeaa)DE-He213 |
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Test performance study to evaluate diagnostic tests for the detection and identification of Xylophilus ampelinus in vine material spiked with cells from X. ampelinus cultures |
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Test performance study to evaluate diagnostic tests for the detection and identification of Xylophilus ampelinus in vine material spiked with cells from X. ampelinus cultures |
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Harrison, Catherine Tomlinson, Jenny Brittain, Ian Laurenson, Lynn van den Berg, Femke |
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test performance study to evaluate diagnostic tests for the detection and identification of xylophilus ampelinus in vine material spiked with cells from x. ampelinus cultures |
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Test performance study to evaluate diagnostic tests for the detection and identification of Xylophilus ampelinus in vine material spiked with cells from X. ampelinus cultures |
| abstract |
Abstract A test performance study (TPS) was conducted in 2020 to evaluate performance of serological and PCR-based tests for the detection of Xylophilus ampelinus in homogenised vine samples. In total, 11 labs participated, although there were fewer participants for the serological tests than the PCR-based tests. The panel of samples sent to participants included spiked samples containing $ 10^{4} $–$ 10^{8} $ cfu per ml (serological tests) or $ 10^{2} $–$ 10^{6} $ cfu per ml (PCR-based tests) as well as positive and negative controls. The five PCR-based tests were found to be fit for purpose, with similar performance across a range of metrics (analytical sensitivity, diagnostic sensitivity and specificity, and repeatability and reproducibility assessed in terms of accordance and concordance, respectively). Serological methods (two immunofluorescence tests and two ELISA tests) were found to be less sensitive with regard to both analytical and diagnostic sensitivity. Furthermore, the occurrence of false positives suggests that a positive IF result may not be conclusive when considered in isolation. One of the ELISA tests exhibited much lower analytical and diagnostic sensitivity than the other serological tests and would not be considered suitable for the purpose considered by this TPS. © Fera Science Ltd. 2023 |
| abstractGer |
Abstract A test performance study (TPS) was conducted in 2020 to evaluate performance of serological and PCR-based tests for the detection of Xylophilus ampelinus in homogenised vine samples. In total, 11 labs participated, although there were fewer participants for the serological tests than the PCR-based tests. The panel of samples sent to participants included spiked samples containing $ 10^{4} $–$ 10^{8} $ cfu per ml (serological tests) or $ 10^{2} $–$ 10^{6} $ cfu per ml (PCR-based tests) as well as positive and negative controls. The five PCR-based tests were found to be fit for purpose, with similar performance across a range of metrics (analytical sensitivity, diagnostic sensitivity and specificity, and repeatability and reproducibility assessed in terms of accordance and concordance, respectively). Serological methods (two immunofluorescence tests and two ELISA tests) were found to be less sensitive with regard to both analytical and diagnostic sensitivity. Furthermore, the occurrence of false positives suggests that a positive IF result may not be conclusive when considered in isolation. One of the ELISA tests exhibited much lower analytical and diagnostic sensitivity than the other serological tests and would not be considered suitable for the purpose considered by this TPS. © Fera Science Ltd. 2023 |
| abstract_unstemmed |
Abstract A test performance study (TPS) was conducted in 2020 to evaluate performance of serological and PCR-based tests for the detection of Xylophilus ampelinus in homogenised vine samples. In total, 11 labs participated, although there were fewer participants for the serological tests than the PCR-based tests. The panel of samples sent to participants included spiked samples containing $ 10^{4} $–$ 10^{8} $ cfu per ml (serological tests) or $ 10^{2} $–$ 10^{6} $ cfu per ml (PCR-based tests) as well as positive and negative controls. The five PCR-based tests were found to be fit for purpose, with similar performance across a range of metrics (analytical sensitivity, diagnostic sensitivity and specificity, and repeatability and reproducibility assessed in terms of accordance and concordance, respectively). Serological methods (two immunofluorescence tests and two ELISA tests) were found to be less sensitive with regard to both analytical and diagnostic sensitivity. Furthermore, the occurrence of false positives suggests that a positive IF result may not be conclusive when considered in isolation. One of the ELISA tests exhibited much lower analytical and diagnostic sensitivity than the other serological tests and would not be considered suitable for the purpose considered by this TPS. © Fera Science Ltd. 2023 |
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| title_short |
Test performance study to evaluate diagnostic tests for the detection and identification of Xylophilus ampelinus in vine material spiked with cells from X. ampelinus cultures |
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https://dx.doi.org/10.1007/s10658-023-02713-z |
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Tomlinson, Jenny Brittain, Ian Laurenson, Lynn van den Berg, Femke |
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| doi_str |
10.1007/s10658-023-02713-z |
| up_date |
2024-07-04T00:33:08.433Z |
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| score |
7.3982544 |