An aging-related immune landscape in the hematopoietic immune system

Background Aging is a holistic change that has a major impact on the immune system, and immunosenescence contributes to the overall progression of aging. The bone marrow is the most important hematopoietic immune organ, while the spleen, as the most important extramedullary hematopoietic immune orga...
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Autor*in:	Lv, Jianjie [verfasserIn] Zhang, Chun Liu, Xiuxing Gu, Chenyang Liu, Yidan Gao, Yuehan Huang, Zhaohao Jiang, Qi Chen, Binyao He, Daquan Wang, Tianfu Xu, Zhuping Su, Wenru

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2024

Schlagwörter:	Hematopoietic immune system Neutrophils Aging Spleen Bone marrow Myeloid cells Hematopoietic stem cells Single-cell sequencing Single-cell flow cytometry

Anmerkung:	© The Author(s) 2023

Übergeordnetes Werk:	Enthalten in: Immunity & ageing - London : BioMed Central, 2004, 21(2024), 1 vom: 02. Jan.
Übergeordnetes Werk:	volume:21 ; year:2024 ; number:1 ; day:02 ; month:01

Links:	Volltext

DOI / URN:	10.1186/s12979-023-00403-2

Katalog-ID:	SPR054233003

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520			\|a Background Aging is a holistic change that has a major impact on the immune system, and immunosenescence contributes to the overall progression of aging. The bone marrow is the most important hematopoietic immune organ, while the spleen, as the most important extramedullary hematopoietic immune organ, maintains homeostasis of the human hematopoietic immune system (HIS) in cooperation with the bone marrow. However, the overall changes in the HIS during aging have not been described. Here, we describe a hematopoietic immune map of the spleen and bone marrow of young and old mice using single-cell sequencing and flow cytometry techniques. Results We observed extensive, complex changes in the HIS during aging. Compared with young mice, the immune cells of aged mice showed a marked tendency toward myeloid differentiation, with the neutrophil population accounting for a significant proportion of this response. In this change, hypoxia-inducible factor 1-alpha (Hif1α) was significantly overexpressed, and this enhanced the immune efficacy and inflammatory response of neutrophils. Our research revealed that during the aging process, hematopoietic stem cells undergo significant changes in function and composition, and their polymorphism and differentiation abilities are downregulated. Moreover, we found that the highly responsive CD62L + HSCs were obviously downregulated in aging, suggesting that they may play an important role in the aging process. Conclusions Overall, aging extensively alters the cellular composition and function of the HIS. These findings could potentially give high-dimensional insights and enable more accurate functional and developmental analyses as well as immune monitoring in HIS aging.
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allfields	10.1186/s12979-023-00403-2 doi (DE-627)SPR054233003 (SPR)s12979-023-00403-2-e DE-627 ger DE-627 rakwb eng Lv, Jianjie verfasserin aut An aging-related immune landscape in the hematopoietic immune system 2024 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s) 2023 Background Aging is a holistic change that has a major impact on the immune system, and immunosenescence contributes to the overall progression of aging. The bone marrow is the most important hematopoietic immune organ, while the spleen, as the most important extramedullary hematopoietic immune organ, maintains homeostasis of the human hematopoietic immune system (HIS) in cooperation with the bone marrow. However, the overall changes in the HIS during aging have not been described. Here, we describe a hematopoietic immune map of the spleen and bone marrow of young and old mice using single-cell sequencing and flow cytometry techniques. Results We observed extensive, complex changes in the HIS during aging. Compared with young mice, the immune cells of aged mice showed a marked tendency toward myeloid differentiation, with the neutrophil population accounting for a significant proportion of this response. In this change, hypoxia-inducible factor 1-alpha (Hif1α) was significantly overexpressed, and this enhanced the immune efficacy and inflammatory response of neutrophils. Our research revealed that during the aging process, hematopoietic stem cells undergo significant changes in function and composition, and their polymorphism and differentiation abilities are downregulated. Moreover, we found that the highly responsive CD62L + HSCs were obviously downregulated in aging, suggesting that they may play an important role in the aging process. Conclusions Overall, aging extensively alters the cellular composition and function of the HIS. These findings could potentially give high-dimensional insights and enable more accurate functional and developmental analyses as well as immune monitoring in HIS aging. Hematopoietic immune system (dpeaa)DE-He213 Neutrophils (dpeaa)DE-He213 Aging (dpeaa)DE-He213 Spleen (dpeaa)DE-He213 Bone marrow (dpeaa)DE-He213 Myeloid cells (dpeaa)DE-He213 Hematopoietic stem cells (dpeaa)DE-He213 Single-cell sequencing (dpeaa)DE-He213 Single-cell flow cytometry (dpeaa)DE-He213 Zhang, Chun aut Liu, Xiuxing aut Gu, Chenyang aut Liu, Yidan aut Gao, Yuehan aut Huang, Zhaohao aut Jiang, Qi aut Chen, Binyao aut He, Daquan aut Wang, Tianfu aut Xu, Zhuping aut Su, Wenru aut Enthalten in Immunity & ageing London : BioMed Central, 2004 21(2024), 1 vom: 02. Jan. (DE-627)473203588 (DE-600)2168941-6 1742-4933 nnns volume:21 year:2024 number:1 day:02 month:01 https://dx.doi.org/10.1186/s12979-023-00403-2 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 21 2024 1 02 01
spelling	10.1186/s12979-023-00403-2 doi (DE-627)SPR054233003 (SPR)s12979-023-00403-2-e DE-627 ger DE-627 rakwb eng Lv, Jianjie verfasserin aut An aging-related immune landscape in the hematopoietic immune system 2024 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s) 2023 Background Aging is a holistic change that has a major impact on the immune system, and immunosenescence contributes to the overall progression of aging. The bone marrow is the most important hematopoietic immune organ, while the spleen, as the most important extramedullary hematopoietic immune organ, maintains homeostasis of the human hematopoietic immune system (HIS) in cooperation with the bone marrow. However, the overall changes in the HIS during aging have not been described. Here, we describe a hematopoietic immune map of the spleen and bone marrow of young and old mice using single-cell sequencing and flow cytometry techniques. Results We observed extensive, complex changes in the HIS during aging. Compared with young mice, the immune cells of aged mice showed a marked tendency toward myeloid differentiation, with the neutrophil population accounting for a significant proportion of this response. In this change, hypoxia-inducible factor 1-alpha (Hif1α) was significantly overexpressed, and this enhanced the immune efficacy and inflammatory response of neutrophils. Our research revealed that during the aging process, hematopoietic stem cells undergo significant changes in function and composition, and their polymorphism and differentiation abilities are downregulated. Moreover, we found that the highly responsive CD62L + HSCs were obviously downregulated in aging, suggesting that they may play an important role in the aging process. Conclusions Overall, aging extensively alters the cellular composition and function of the HIS. These findings could potentially give high-dimensional insights and enable more accurate functional and developmental analyses as well as immune monitoring in HIS aging. Hematopoietic immune system (dpeaa)DE-He213 Neutrophils (dpeaa)DE-He213 Aging (dpeaa)DE-He213 Spleen (dpeaa)DE-He213 Bone marrow (dpeaa)DE-He213 Myeloid cells (dpeaa)DE-He213 Hematopoietic stem cells (dpeaa)DE-He213 Single-cell sequencing (dpeaa)DE-He213 Single-cell flow cytometry (dpeaa)DE-He213 Zhang, Chun aut Liu, Xiuxing aut Gu, Chenyang aut Liu, Yidan aut Gao, Yuehan aut Huang, Zhaohao aut Jiang, Qi aut Chen, Binyao aut He, Daquan aut Wang, Tianfu aut Xu, Zhuping aut Su, Wenru aut Enthalten in Immunity & ageing London : BioMed Central, 2004 21(2024), 1 vom: 02. Jan. (DE-627)473203588 (DE-600)2168941-6 1742-4933 nnns volume:21 year:2024 number:1 day:02 month:01 https://dx.doi.org/10.1186/s12979-023-00403-2 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 21 2024 1 02 01
allfields_unstemmed	10.1186/s12979-023-00403-2 doi (DE-627)SPR054233003 (SPR)s12979-023-00403-2-e DE-627 ger DE-627 rakwb eng Lv, Jianjie verfasserin aut An aging-related immune landscape in the hematopoietic immune system 2024 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s) 2023 Background Aging is a holistic change that has a major impact on the immune system, and immunosenescence contributes to the overall progression of aging. The bone marrow is the most important hematopoietic immune organ, while the spleen, as the most important extramedullary hematopoietic immune organ, maintains homeostasis of the human hematopoietic immune system (HIS) in cooperation with the bone marrow. However, the overall changes in the HIS during aging have not been described. Here, we describe a hematopoietic immune map of the spleen and bone marrow of young and old mice using single-cell sequencing and flow cytometry techniques. Results We observed extensive, complex changes in the HIS during aging. Compared with young mice, the immune cells of aged mice showed a marked tendency toward myeloid differentiation, with the neutrophil population accounting for a significant proportion of this response. In this change, hypoxia-inducible factor 1-alpha (Hif1α) was significantly overexpressed, and this enhanced the immune efficacy and inflammatory response of neutrophils. Our research revealed that during the aging process, hematopoietic stem cells undergo significant changes in function and composition, and their polymorphism and differentiation abilities are downregulated. Moreover, we found that the highly responsive CD62L + HSCs were obviously downregulated in aging, suggesting that they may play an important role in the aging process. Conclusions Overall, aging extensively alters the cellular composition and function of the HIS. These findings could potentially give high-dimensional insights and enable more accurate functional and developmental analyses as well as immune monitoring in HIS aging. Hematopoietic immune system (dpeaa)DE-He213 Neutrophils (dpeaa)DE-He213 Aging (dpeaa)DE-He213 Spleen (dpeaa)DE-He213 Bone marrow (dpeaa)DE-He213 Myeloid cells (dpeaa)DE-He213 Hematopoietic stem cells (dpeaa)DE-He213 Single-cell sequencing (dpeaa)DE-He213 Single-cell flow cytometry (dpeaa)DE-He213 Zhang, Chun aut Liu, Xiuxing aut Gu, Chenyang aut Liu, Yidan aut Gao, Yuehan aut Huang, Zhaohao aut Jiang, Qi aut Chen, Binyao aut He, Daquan aut Wang, Tianfu aut Xu, Zhuping aut Su, Wenru aut Enthalten in Immunity & ageing London : BioMed Central, 2004 21(2024), 1 vom: 02. Jan. (DE-627)473203588 (DE-600)2168941-6 1742-4933 nnns volume:21 year:2024 number:1 day:02 month:01 https://dx.doi.org/10.1186/s12979-023-00403-2 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 21 2024 1 02 01
allfieldsGer	10.1186/s12979-023-00403-2 doi (DE-627)SPR054233003 (SPR)s12979-023-00403-2-e DE-627 ger DE-627 rakwb eng Lv, Jianjie verfasserin aut An aging-related immune landscape in the hematopoietic immune system 2024 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s) 2023 Background Aging is a holistic change that has a major impact on the immune system, and immunosenescence contributes to the overall progression of aging. The bone marrow is the most important hematopoietic immune organ, while the spleen, as the most important extramedullary hematopoietic immune organ, maintains homeostasis of the human hematopoietic immune system (HIS) in cooperation with the bone marrow. However, the overall changes in the HIS during aging have not been described. Here, we describe a hematopoietic immune map of the spleen and bone marrow of young and old mice using single-cell sequencing and flow cytometry techniques. Results We observed extensive, complex changes in the HIS during aging. Compared with young mice, the immune cells of aged mice showed a marked tendency toward myeloid differentiation, with the neutrophil population accounting for a significant proportion of this response. In this change, hypoxia-inducible factor 1-alpha (Hif1α) was significantly overexpressed, and this enhanced the immune efficacy and inflammatory response of neutrophils. Our research revealed that during the aging process, hematopoietic stem cells undergo significant changes in function and composition, and their polymorphism and differentiation abilities are downregulated. Moreover, we found that the highly responsive CD62L + HSCs were obviously downregulated in aging, suggesting that they may play an important role in the aging process. Conclusions Overall, aging extensively alters the cellular composition and function of the HIS. These findings could potentially give high-dimensional insights and enable more accurate functional and developmental analyses as well as immune monitoring in HIS aging. Hematopoietic immune system (dpeaa)DE-He213 Neutrophils (dpeaa)DE-He213 Aging (dpeaa)DE-He213 Spleen (dpeaa)DE-He213 Bone marrow (dpeaa)DE-He213 Myeloid cells (dpeaa)DE-He213 Hematopoietic stem cells (dpeaa)DE-He213 Single-cell sequencing (dpeaa)DE-He213 Single-cell flow cytometry (dpeaa)DE-He213 Zhang, Chun aut Liu, Xiuxing aut Gu, Chenyang aut Liu, Yidan aut Gao, Yuehan aut Huang, Zhaohao aut Jiang, Qi aut Chen, Binyao aut He, Daquan aut Wang, Tianfu aut Xu, Zhuping aut Su, Wenru aut Enthalten in Immunity & ageing London : BioMed Central, 2004 21(2024), 1 vom: 02. Jan. (DE-627)473203588 (DE-600)2168941-6 1742-4933 nnns volume:21 year:2024 number:1 day:02 month:01 https://dx.doi.org/10.1186/s12979-023-00403-2 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 21 2024 1 02 01
allfieldsSound	10.1186/s12979-023-00403-2 doi (DE-627)SPR054233003 (SPR)s12979-023-00403-2-e DE-627 ger DE-627 rakwb eng Lv, Jianjie verfasserin aut An aging-related immune landscape in the hematopoietic immune system 2024 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s) 2023 Background Aging is a holistic change that has a major impact on the immune system, and immunosenescence contributes to the overall progression of aging. The bone marrow is the most important hematopoietic immune organ, while the spleen, as the most important extramedullary hematopoietic immune organ, maintains homeostasis of the human hematopoietic immune system (HIS) in cooperation with the bone marrow. However, the overall changes in the HIS during aging have not been described. Here, we describe a hematopoietic immune map of the spleen and bone marrow of young and old mice using single-cell sequencing and flow cytometry techniques. Results We observed extensive, complex changes in the HIS during aging. Compared with young mice, the immune cells of aged mice showed a marked tendency toward myeloid differentiation, with the neutrophil population accounting for a significant proportion of this response. In this change, hypoxia-inducible factor 1-alpha (Hif1α) was significantly overexpressed, and this enhanced the immune efficacy and inflammatory response of neutrophils. Our research revealed that during the aging process, hematopoietic stem cells undergo significant changes in function and composition, and their polymorphism and differentiation abilities are downregulated. Moreover, we found that the highly responsive CD62L + HSCs were obviously downregulated in aging, suggesting that they may play an important role in the aging process. Conclusions Overall, aging extensively alters the cellular composition and function of the HIS. These findings could potentially give high-dimensional insights and enable more accurate functional and developmental analyses as well as immune monitoring in HIS aging. Hematopoietic immune system (dpeaa)DE-He213 Neutrophils (dpeaa)DE-He213 Aging (dpeaa)DE-He213 Spleen (dpeaa)DE-He213 Bone marrow (dpeaa)DE-He213 Myeloid cells (dpeaa)DE-He213 Hematopoietic stem cells (dpeaa)DE-He213 Single-cell sequencing (dpeaa)DE-He213 Single-cell flow cytometry (dpeaa)DE-He213 Zhang, Chun aut Liu, Xiuxing aut Gu, Chenyang aut Liu, Yidan aut Gao, Yuehan aut Huang, Zhaohao aut Jiang, Qi aut Chen, Binyao aut He, Daquan aut Wang, Tianfu aut Xu, Zhuping aut Su, Wenru aut Enthalten in Immunity & ageing London : BioMed Central, 2004 21(2024), 1 vom: 02. Jan. (DE-627)473203588 (DE-600)2168941-6 1742-4933 nnns volume:21 year:2024 number:1 day:02 month:01 https://dx.doi.org/10.1186/s12979-023-00403-2 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 21 2024 1 02 01
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author	Lv, Jianjie
spellingShingle	Lv, Jianjie misc Hematopoietic immune system misc Neutrophils misc Aging misc Spleen misc Bone marrow misc Myeloid cells misc Hematopoietic stem cells misc Single-cell sequencing misc Single-cell flow cytometry An aging-related immune landscape in the hematopoietic immune system
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topic_title	An aging-related immune landscape in the hematopoietic immune system Hematopoietic immune system (dpeaa)DE-He213 Neutrophils (dpeaa)DE-He213 Aging (dpeaa)DE-He213 Spleen (dpeaa)DE-He213 Bone marrow (dpeaa)DE-He213 Myeloid cells (dpeaa)DE-He213 Hematopoietic stem cells (dpeaa)DE-He213 Single-cell sequencing (dpeaa)DE-He213 Single-cell flow cytometry (dpeaa)DE-He213
topic	misc Hematopoietic immune system misc Neutrophils misc Aging misc Spleen misc Bone marrow misc Myeloid cells misc Hematopoietic stem cells misc Single-cell sequencing misc Single-cell flow cytometry
topic_unstemmed	misc Hematopoietic immune system misc Neutrophils misc Aging misc Spleen misc Bone marrow misc Myeloid cells misc Hematopoietic stem cells misc Single-cell sequencing misc Single-cell flow cytometry
topic_browse	misc Hematopoietic immune system misc Neutrophils misc Aging misc Spleen misc Bone marrow misc Myeloid cells misc Hematopoietic stem cells misc Single-cell sequencing misc Single-cell flow cytometry
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title	An aging-related immune landscape in the hematopoietic immune system
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author_browse	Lv, Jianjie Zhang, Chun Liu, Xiuxing Gu, Chenyang Liu, Yidan Gao, Yuehan Huang, Zhaohao Jiang, Qi Chen, Binyao He, Daquan Wang, Tianfu Xu, Zhuping Su, Wenru
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title_sort	aging-related immune landscape in the hematopoietic immune system
title_auth	An aging-related immune landscape in the hematopoietic immune system
abstract	Background Aging is a holistic change that has a major impact on the immune system, and immunosenescence contributes to the overall progression of aging. The bone marrow is the most important hematopoietic immune organ, while the spleen, as the most important extramedullary hematopoietic immune organ, maintains homeostasis of the human hematopoietic immune system (HIS) in cooperation with the bone marrow. However, the overall changes in the HIS during aging have not been described. Here, we describe a hematopoietic immune map of the spleen and bone marrow of young and old mice using single-cell sequencing and flow cytometry techniques. Results We observed extensive, complex changes in the HIS during aging. Compared with young mice, the immune cells of aged mice showed a marked tendency toward myeloid differentiation, with the neutrophil population accounting for a significant proportion of this response. In this change, hypoxia-inducible factor 1-alpha (Hif1α) was significantly overexpressed, and this enhanced the immune efficacy and inflammatory response of neutrophils. Our research revealed that during the aging process, hematopoietic stem cells undergo significant changes in function and composition, and their polymorphism and differentiation abilities are downregulated. Moreover, we found that the highly responsive CD62L + HSCs were obviously downregulated in aging, suggesting that they may play an important role in the aging process. Conclusions Overall, aging extensively alters the cellular composition and function of the HIS. These findings could potentially give high-dimensional insights and enable more accurate functional and developmental analyses as well as immune monitoring in HIS aging. © The Author(s) 2023
abstractGer	Background Aging is a holistic change that has a major impact on the immune system, and immunosenescence contributes to the overall progression of aging. The bone marrow is the most important hematopoietic immune organ, while the spleen, as the most important extramedullary hematopoietic immune organ, maintains homeostasis of the human hematopoietic immune system (HIS) in cooperation with the bone marrow. However, the overall changes in the HIS during aging have not been described. Here, we describe a hematopoietic immune map of the spleen and bone marrow of young and old mice using single-cell sequencing and flow cytometry techniques. Results We observed extensive, complex changes in the HIS during aging. Compared with young mice, the immune cells of aged mice showed a marked tendency toward myeloid differentiation, with the neutrophil population accounting for a significant proportion of this response. In this change, hypoxia-inducible factor 1-alpha (Hif1α) was significantly overexpressed, and this enhanced the immune efficacy and inflammatory response of neutrophils. Our research revealed that during the aging process, hematopoietic stem cells undergo significant changes in function and composition, and their polymorphism and differentiation abilities are downregulated. Moreover, we found that the highly responsive CD62L + HSCs were obviously downregulated in aging, suggesting that they may play an important role in the aging process. Conclusions Overall, aging extensively alters the cellular composition and function of the HIS. These findings could potentially give high-dimensional insights and enable more accurate functional and developmental analyses as well as immune monitoring in HIS aging. © The Author(s) 2023
abstract_unstemmed	Background Aging is a holistic change that has a major impact on the immune system, and immunosenescence contributes to the overall progression of aging. The bone marrow is the most important hematopoietic immune organ, while the spleen, as the most important extramedullary hematopoietic immune organ, maintains homeostasis of the human hematopoietic immune system (HIS) in cooperation with the bone marrow. However, the overall changes in the HIS during aging have not been described. Here, we describe a hematopoietic immune map of the spleen and bone marrow of young and old mice using single-cell sequencing and flow cytometry techniques. Results We observed extensive, complex changes in the HIS during aging. Compared with young mice, the immune cells of aged mice showed a marked tendency toward myeloid differentiation, with the neutrophil population accounting for a significant proportion of this response. In this change, hypoxia-inducible factor 1-alpha (Hif1α) was significantly overexpressed, and this enhanced the immune efficacy and inflammatory response of neutrophils. Our research revealed that during the aging process, hematopoietic stem cells undergo significant changes in function and composition, and their polymorphism and differentiation abilities are downregulated. Moreover, we found that the highly responsive CD62L + HSCs were obviously downregulated in aging, suggesting that they may play an important role in the aging process. Conclusions Overall, aging extensively alters the cellular composition and function of the HIS. These findings could potentially give high-dimensional insights and enable more accurate functional and developmental analyses as well as immune monitoring in HIS aging. © The Author(s) 2023
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title_short	An aging-related immune landscape in the hematopoietic immune system
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author2	Zhang, Chun Liu, Xiuxing Gu, Chenyang Liu, Yidan Gao, Yuehan Huang, Zhaohao Jiang, Qi Chen, Binyao He, Daquan Wang, Tianfu Xu, Zhuping Su, Wenru
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The bone marrow is the most important hematopoietic immune organ, while the spleen, as the most important extramedullary hematopoietic immune organ, maintains homeostasis of the human hematopoietic immune system (HIS) in cooperation with the bone marrow. However, the overall changes in the HIS during aging have not been described. Here, we describe a hematopoietic immune map of the spleen and bone marrow of young and old mice using single-cell sequencing and flow cytometry techniques. Results We observed extensive, complex changes in the HIS during aging. Compared with young mice, the immune cells of aged mice showed a marked tendency toward myeloid differentiation, with the neutrophil population accounting for a significant proportion of this response. In this change, hypoxia-inducible factor 1-alpha (Hif1α) was significantly overexpressed, and this enhanced the immune efficacy and inflammatory response of neutrophils. Our research revealed that during the aging process, hematopoietic stem cells undergo significant changes in function and composition, and their polymorphism and differentiation abilities are downregulated. Moreover, we found that the highly responsive CD62L + HSCs were obviously downregulated in aging, suggesting that they may play an important role in the aging process. Conclusions Overall, aging extensively alters the cellular composition and function of the HIS. These findings could potentially give high-dimensional insights and enable more accurate functional and developmental analyses as well as immune monitoring in HIS aging.</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Hematopoietic immune system</subfield><subfield code="7">(dpeaa)DE-He213</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Neutrophils</subfield><subfield code="7">(dpeaa)DE-He213</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Aging</subfield><subfield code="7">(dpeaa)DE-He213</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Spleen</subfield><subfield code="7">(dpeaa)DE-He213</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Bone marrow</subfield><subfield code="7">(dpeaa)DE-He213</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Myeloid cells</subfield><subfield code="7">(dpeaa)DE-He213</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Hematopoietic stem cells</subfield><subfield code="7">(dpeaa)DE-He213</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Single-cell sequencing</subfield><subfield code="7">(dpeaa)DE-He213</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Single-cell flow cytometry</subfield><subfield code="7">(dpeaa)DE-He213</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Zhang, Chun</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Liu, Xiuxing</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Gu, Chenyang</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Liu, Yidan</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Gao, Yuehan</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Huang, Zhaohao</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Jiang, Qi</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Chen, Binyao</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">He, Daquan</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Wang, Tianfu</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Xu, Zhuping</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Su, Wenru</subfield><subfield code="4">aut</subfield></datafield><datafield tag="773" ind1="0" ind2="8"><subfield code="i">Enthalten in</subfield><subfield code="t">Immunity & ageing</subfield><subfield code="d">London : BioMed Central, 2004</subfield><subfield code="g">21(2024), 1 vom: 02. 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An aging-related immune landscape in the hematopoietic immune system

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