Spatial distribution characteristics of denitrification functional genes and the environmental drivers in Liaohe estuary wetland

Abstract Genes nirS, nirK, and nosZ are specific for the denitrification process, which is associated with greenhouse gas $ N_{2} $O emission. The abundances and diversities of community containing these three genes are usually used as a common index to reflect the denitrification process, and they...
Ausführliche Beschreibung

Abstract Genes nirS, nirK, and nosZ are specific for the denitrification process, which is associated with greenhouse gas $ N_{2} $O emission. The abundances and diversities of community containing these three genes are usually used as a common index to reflect the denitrification process, and they would be affected by differences in environmental factors caused by changes from warm to cold conditions. The quantification of denitrification in natural wetlands is complex, and straightforward identification of spatial distribution and drivers affecting the process is still developing. In this study, the bacterial communities, gene diversities, and relative abundances involved in denitrification were investigated in Liaohe Estuary Wetland. We analyzed the relative abundances, diversities, and communities of bacteria containing the three genes at warm and cold conditions using Illumina MiSeq sequencing and detected the potential environmental factors influencing their distribution by using a random forest algorithm. There are great differences in the community composition of the bacteria containing genes nirS, nirK, and nosZ. All the abundant taxa of nirS and nirK communities belonged to phylum Proteobacteria. Compared with the community composition of bacteria containing nirS and nirK, the community of bacteria containing nosZ is more diverse, and the subdivision taxa of phylum Euryarchaeota was also abundant in the community containing nosZ. The distribution characteristics of the relative abundance of nirS and nirK showed obvious differences both at warm and cold climate conditions. The oxidation–reduction potential, nitrite nitrogen, and salinity were detected as potential variables that might explain the diversity of nirS. The total nitrogen and nitrite nitrogen were the important variables for predicting the relative abundance of nirS at warm climate condition, while oxidation–reduction potential and pH contributed to the diversity of nirS at cold condition. The bulk density of sediment was detected as a potential variable affecting the relative abundance of nirK at warm and cold conditions, and diversity of nirK at warm condition, while nitrite nitrogen was detected as an important environmental factor for predicting the diversity of nirK at cold condition. Overall, our results show that the key environmental factors, which affect the relative abundance, diversity, and community of bacteria containing the functional denitrification genes, are not exactly the same, and the diversities of nirS, nirK, and nosZ have a higher environmental sensitivity than their relative abundances. Ausführliche Beschreibung

Autor*in:	Wei, Chao [verfasserIn] Su, Fangli Yue, Hangyu Song, Fei Li, Haifu

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2023

Schlagwörter:	Liaohe Estuary Wetland Denitrification Functional gene Microbial community

Anmerkung:

© The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature 2023. Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.

Übergeordnetes Werk:	Enthalten in: Environmental science and pollution research - Berlin : Springer, 1994, 31(2023), 1 vom: 30. Nov., Seite 1064-1078
Übergeordnetes Werk:	volume:31 ; year:2023 ; number:1 ; day:30 ; month:11 ; pages:1064-1078

Links:	Volltext

DOI / URN:	10.1007/s11356-023-30938-2

Katalog-ID:	SPR054394023