Prodigiosin production and recovery from Serratia marcescens: process development and cost–benefit analysis
Abstract Prodigiosin is a tripyrrole red pigment that has been found to have antimicrobial, anticancer, and immunosuppressive properties. Experiments were conducted in this study to determine the best media types, temperature, and nitrogen and carbon sources for improving prodigiosin intracellular y...
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| Autor*in: |
Paul, Tania [verfasserIn] |
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| Format: |
E-Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
2022 |
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| Schlagwörter: |
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| Anmerkung: |
© The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature 2022 |
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| Übergeordnetes Werk: |
Enthalten in: Biomass Conversion and Biorefinery - Berlin : Springer, 2011, 14(2022), 3 vom: 06. Apr., Seite 4091-4110 |
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| Übergeordnetes Werk: |
volume:14 ; year:2022 ; number:3 ; day:06 ; month:04 ; pages:4091-4110 |
| Links: |
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| DOI / URN: |
10.1007/s13399-022-02639-2 |
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SPR054569389 |
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| 520 | |a Abstract Prodigiosin is a tripyrrole red pigment that has been found to have antimicrobial, anticancer, and immunosuppressive properties. Experiments were conducted in this study to determine the best media types, temperature, and nitrogen and carbon sources for improving prodigiosin intracellular yield in Serratia marcescens. The nutrient broth (NB) medium at 30 °C yielded the highest level of prodigiosin (15 mg/L) when compared to peptone glycerol (PG), tryptone soy broth (TSB), Luria–Bertani broth (LBB), and glycerol beef broth (GBB). Lactose and peptone were found to be the most effective carbon and nitrogen sources, and when added to the nutrient broth, prodigiosin production increased to 18.67% (17.8 mg/L) and 22.67% (18.4 mg/L), respectively. To extract prodigiosin from Serratia marcescens biomass, the best ratio of organic solvent to water (pH 3) was 19: 1, and ethanol was found to be the best organic solvent to extract prodigiosin (15 mg/L) among DMSO, methanol, acetone, acetonitrile, and chloroform. To purify the prodigiosin, a column chromatography study was conducted, with Silica Gel 60–120 mesh as the stationary phase and a solvent mixture of chloroform, methanol, and acetone (4:2:3 v/v) as the mobile phase, yielding a purification yield of 65.39%. The absorption maximum of prodigiosin was found to be 535 nm in a UV–VIS study, and FTIR study confirmed that the functional group of prodigiosin is well matched with prior studies. The eluted fraction of the column contained 100% pure prodigiosin, which was validated by HPLC analysis. The LC–MS analysis also confirmed that prodigiosin was present in the eluted fraction of the column, with a peak at 324 m/z. When compared to the commercial pricing of prodigiosin on the worldwide market, modified media I (nutrient broth with 5% peanut), modified media II (nutrient broth with 5% sesame), and conventional media (nutrient broth) were determined to be economically feasible. The total saleable costs for 1 mg of prodigiosin obtained from modified media I, modified media II, and conventional media are 9229.41 INR (120.68 $), 14,502.66 INR (189.63 $), and 20,301.46 INR (265.45 $) respectively. The economic gain obtained from the modified media I, modified media II, and conventional media was found to be 362.27 $, 293.32 $, and 217.50 $ per mg of prodigiosin respectively. | ||
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| 700 | 1 | |a Bandyopadhyay, Tarun Kanti |4 aut | |
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10.1007/s13399-022-02639-2 doi (DE-627)SPR054569389 (SPR)s13399-022-02639-2-e DE-627 ger DE-627 rakwb eng Paul, Tania verfasserin aut Prodigiosin production and recovery from Serratia marcescens: process development and cost–benefit analysis 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature 2022 Abstract Prodigiosin is a tripyrrole red pigment that has been found to have antimicrobial, anticancer, and immunosuppressive properties. Experiments were conducted in this study to determine the best media types, temperature, and nitrogen and carbon sources for improving prodigiosin intracellular yield in Serratia marcescens. The nutrient broth (NB) medium at 30 °C yielded the highest level of prodigiosin (15 mg/L) when compared to peptone glycerol (PG), tryptone soy broth (TSB), Luria–Bertani broth (LBB), and glycerol beef broth (GBB). Lactose and peptone were found to be the most effective carbon and nitrogen sources, and when added to the nutrient broth, prodigiosin production increased to 18.67% (17.8 mg/L) and 22.67% (18.4 mg/L), respectively. To extract prodigiosin from Serratia marcescens biomass, the best ratio of organic solvent to water (pH 3) was 19: 1, and ethanol was found to be the best organic solvent to extract prodigiosin (15 mg/L) among DMSO, methanol, acetone, acetonitrile, and chloroform. To purify the prodigiosin, a column chromatography study was conducted, with Silica Gel 60–120 mesh as the stationary phase and a solvent mixture of chloroform, methanol, and acetone (4:2:3 v/v) as the mobile phase, yielding a purification yield of 65.39%. The absorption maximum of prodigiosin was found to be 535 nm in a UV–VIS study, and FTIR study confirmed that the functional group of prodigiosin is well matched with prior studies. The eluted fraction of the column contained 100% pure prodigiosin, which was validated by HPLC analysis. The LC–MS analysis also confirmed that prodigiosin was present in the eluted fraction of the column, with a peak at 324 m/z. When compared to the commercial pricing of prodigiosin on the worldwide market, modified media I (nutrient broth with 5% peanut), modified media II (nutrient broth with 5% sesame), and conventional media (nutrient broth) were determined to be economically feasible. The total saleable costs for 1 mg of prodigiosin obtained from modified media I, modified media II, and conventional media are 9229.41 INR (120.68 $), 14,502.66 INR (189.63 $), and 20,301.46 INR (265.45 $) respectively. The economic gain obtained from the modified media I, modified media II, and conventional media was found to be 362.27 $, 293.32 $, and 217.50 $ per mg of prodigiosin respectively. Column chromatography (dpeaa)DE-He213 Cost–benefit analysis (dpeaa)DE-He213 Prodigiosin (dpeaa)DE-He213 Extraction (dpeaa)DE-He213 Purification, characterization (dpeaa)DE-He213 Mondal, Abhijit aut Bandyopadhyay, Tarun Kanti aut Bhunia, Biswanath (orcid)0000-0003-1330-1606 aut Enthalten in Biomass Conversion and Biorefinery Berlin : Springer, 2011 14(2022), 3 vom: 06. Apr., Seite 4091-4110 (DE-627)645092843 (DE-600)2592298-1 2190-6823 nnns volume:14 year:2022 number:3 day:06 month:04 pages:4091-4110 https://dx.doi.org/10.1007/s13399-022-02639-2 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 14 2022 3 06 04 4091-4110 |
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10.1007/s13399-022-02639-2 doi (DE-627)SPR054569389 (SPR)s13399-022-02639-2-e DE-627 ger DE-627 rakwb eng Paul, Tania verfasserin aut Prodigiosin production and recovery from Serratia marcescens: process development and cost–benefit analysis 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature 2022 Abstract Prodigiosin is a tripyrrole red pigment that has been found to have antimicrobial, anticancer, and immunosuppressive properties. Experiments were conducted in this study to determine the best media types, temperature, and nitrogen and carbon sources for improving prodigiosin intracellular yield in Serratia marcescens. The nutrient broth (NB) medium at 30 °C yielded the highest level of prodigiosin (15 mg/L) when compared to peptone glycerol (PG), tryptone soy broth (TSB), Luria–Bertani broth (LBB), and glycerol beef broth (GBB). Lactose and peptone were found to be the most effective carbon and nitrogen sources, and when added to the nutrient broth, prodigiosin production increased to 18.67% (17.8 mg/L) and 22.67% (18.4 mg/L), respectively. To extract prodigiosin from Serratia marcescens biomass, the best ratio of organic solvent to water (pH 3) was 19: 1, and ethanol was found to be the best organic solvent to extract prodigiosin (15 mg/L) among DMSO, methanol, acetone, acetonitrile, and chloroform. To purify the prodigiosin, a column chromatography study was conducted, with Silica Gel 60–120 mesh as the stationary phase and a solvent mixture of chloroform, methanol, and acetone (4:2:3 v/v) as the mobile phase, yielding a purification yield of 65.39%. The absorption maximum of prodigiosin was found to be 535 nm in a UV–VIS study, and FTIR study confirmed that the functional group of prodigiosin is well matched with prior studies. The eluted fraction of the column contained 100% pure prodigiosin, which was validated by HPLC analysis. The LC–MS analysis also confirmed that prodigiosin was present in the eluted fraction of the column, with a peak at 324 m/z. When compared to the commercial pricing of prodigiosin on the worldwide market, modified media I (nutrient broth with 5% peanut), modified media II (nutrient broth with 5% sesame), and conventional media (nutrient broth) were determined to be economically feasible. The total saleable costs for 1 mg of prodigiosin obtained from modified media I, modified media II, and conventional media are 9229.41 INR (120.68 $), 14,502.66 INR (189.63 $), and 20,301.46 INR (265.45 $) respectively. The economic gain obtained from the modified media I, modified media II, and conventional media was found to be 362.27 $, 293.32 $, and 217.50 $ per mg of prodigiosin respectively. Column chromatography (dpeaa)DE-He213 Cost–benefit analysis (dpeaa)DE-He213 Prodigiosin (dpeaa)DE-He213 Extraction (dpeaa)DE-He213 Purification, characterization (dpeaa)DE-He213 Mondal, Abhijit aut Bandyopadhyay, Tarun Kanti aut Bhunia, Biswanath (orcid)0000-0003-1330-1606 aut Enthalten in Biomass Conversion and Biorefinery Berlin : Springer, 2011 14(2022), 3 vom: 06. Apr., Seite 4091-4110 (DE-627)645092843 (DE-600)2592298-1 2190-6823 nnns volume:14 year:2022 number:3 day:06 month:04 pages:4091-4110 https://dx.doi.org/10.1007/s13399-022-02639-2 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 14 2022 3 06 04 4091-4110 |
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10.1007/s13399-022-02639-2 doi (DE-627)SPR054569389 (SPR)s13399-022-02639-2-e DE-627 ger DE-627 rakwb eng Paul, Tania verfasserin aut Prodigiosin production and recovery from Serratia marcescens: process development and cost–benefit analysis 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature 2022 Abstract Prodigiosin is a tripyrrole red pigment that has been found to have antimicrobial, anticancer, and immunosuppressive properties. Experiments were conducted in this study to determine the best media types, temperature, and nitrogen and carbon sources for improving prodigiosin intracellular yield in Serratia marcescens. The nutrient broth (NB) medium at 30 °C yielded the highest level of prodigiosin (15 mg/L) when compared to peptone glycerol (PG), tryptone soy broth (TSB), Luria–Bertani broth (LBB), and glycerol beef broth (GBB). Lactose and peptone were found to be the most effective carbon and nitrogen sources, and when added to the nutrient broth, prodigiosin production increased to 18.67% (17.8 mg/L) and 22.67% (18.4 mg/L), respectively. To extract prodigiosin from Serratia marcescens biomass, the best ratio of organic solvent to water (pH 3) was 19: 1, and ethanol was found to be the best organic solvent to extract prodigiosin (15 mg/L) among DMSO, methanol, acetone, acetonitrile, and chloroform. To purify the prodigiosin, a column chromatography study was conducted, with Silica Gel 60–120 mesh as the stationary phase and a solvent mixture of chloroform, methanol, and acetone (4:2:3 v/v) as the mobile phase, yielding a purification yield of 65.39%. The absorption maximum of prodigiosin was found to be 535 nm in a UV–VIS study, and FTIR study confirmed that the functional group of prodigiosin is well matched with prior studies. The eluted fraction of the column contained 100% pure prodigiosin, which was validated by HPLC analysis. The LC–MS analysis also confirmed that prodigiosin was present in the eluted fraction of the column, with a peak at 324 m/z. When compared to the commercial pricing of prodigiosin on the worldwide market, modified media I (nutrient broth with 5% peanut), modified media II (nutrient broth with 5% sesame), and conventional media (nutrient broth) were determined to be economically feasible. The total saleable costs for 1 mg of prodigiosin obtained from modified media I, modified media II, and conventional media are 9229.41 INR (120.68 $), 14,502.66 INR (189.63 $), and 20,301.46 INR (265.45 $) respectively. The economic gain obtained from the modified media I, modified media II, and conventional media was found to be 362.27 $, 293.32 $, and 217.50 $ per mg of prodigiosin respectively. Column chromatography (dpeaa)DE-He213 Cost–benefit analysis (dpeaa)DE-He213 Prodigiosin (dpeaa)DE-He213 Extraction (dpeaa)DE-He213 Purification, characterization (dpeaa)DE-He213 Mondal, Abhijit aut Bandyopadhyay, Tarun Kanti aut Bhunia, Biswanath (orcid)0000-0003-1330-1606 aut Enthalten in Biomass Conversion and Biorefinery Berlin : Springer, 2011 14(2022), 3 vom: 06. Apr., Seite 4091-4110 (DE-627)645092843 (DE-600)2592298-1 2190-6823 nnns volume:14 year:2022 number:3 day:06 month:04 pages:4091-4110 https://dx.doi.org/10.1007/s13399-022-02639-2 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 14 2022 3 06 04 4091-4110 |
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10.1007/s13399-022-02639-2 doi (DE-627)SPR054569389 (SPR)s13399-022-02639-2-e DE-627 ger DE-627 rakwb eng Paul, Tania verfasserin aut Prodigiosin production and recovery from Serratia marcescens: process development and cost–benefit analysis 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature 2022 Abstract Prodigiosin is a tripyrrole red pigment that has been found to have antimicrobial, anticancer, and immunosuppressive properties. Experiments were conducted in this study to determine the best media types, temperature, and nitrogen and carbon sources for improving prodigiosin intracellular yield in Serratia marcescens. The nutrient broth (NB) medium at 30 °C yielded the highest level of prodigiosin (15 mg/L) when compared to peptone glycerol (PG), tryptone soy broth (TSB), Luria–Bertani broth (LBB), and glycerol beef broth (GBB). Lactose and peptone were found to be the most effective carbon and nitrogen sources, and when added to the nutrient broth, prodigiosin production increased to 18.67% (17.8 mg/L) and 22.67% (18.4 mg/L), respectively. To extract prodigiosin from Serratia marcescens biomass, the best ratio of organic solvent to water (pH 3) was 19: 1, and ethanol was found to be the best organic solvent to extract prodigiosin (15 mg/L) among DMSO, methanol, acetone, acetonitrile, and chloroform. To purify the prodigiosin, a column chromatography study was conducted, with Silica Gel 60–120 mesh as the stationary phase and a solvent mixture of chloroform, methanol, and acetone (4:2:3 v/v) as the mobile phase, yielding a purification yield of 65.39%. The absorption maximum of prodigiosin was found to be 535 nm in a UV–VIS study, and FTIR study confirmed that the functional group of prodigiosin is well matched with prior studies. The eluted fraction of the column contained 100% pure prodigiosin, which was validated by HPLC analysis. The LC–MS analysis also confirmed that prodigiosin was present in the eluted fraction of the column, with a peak at 324 m/z. When compared to the commercial pricing of prodigiosin on the worldwide market, modified media I (nutrient broth with 5% peanut), modified media II (nutrient broth with 5% sesame), and conventional media (nutrient broth) were determined to be economically feasible. The total saleable costs for 1 mg of prodigiosin obtained from modified media I, modified media II, and conventional media are 9229.41 INR (120.68 $), 14,502.66 INR (189.63 $), and 20,301.46 INR (265.45 $) respectively. The economic gain obtained from the modified media I, modified media II, and conventional media was found to be 362.27 $, 293.32 $, and 217.50 $ per mg of prodigiosin respectively. Column chromatography (dpeaa)DE-He213 Cost–benefit analysis (dpeaa)DE-He213 Prodigiosin (dpeaa)DE-He213 Extraction (dpeaa)DE-He213 Purification, characterization (dpeaa)DE-He213 Mondal, Abhijit aut Bandyopadhyay, Tarun Kanti aut Bhunia, Biswanath (orcid)0000-0003-1330-1606 aut Enthalten in Biomass Conversion and Biorefinery Berlin : Springer, 2011 14(2022), 3 vom: 06. Apr., Seite 4091-4110 (DE-627)645092843 (DE-600)2592298-1 2190-6823 nnns volume:14 year:2022 number:3 day:06 month:04 pages:4091-4110 https://dx.doi.org/10.1007/s13399-022-02639-2 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 14 2022 3 06 04 4091-4110 |
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10.1007/s13399-022-02639-2 doi (DE-627)SPR054569389 (SPR)s13399-022-02639-2-e DE-627 ger DE-627 rakwb eng Paul, Tania verfasserin aut Prodigiosin production and recovery from Serratia marcescens: process development and cost–benefit analysis 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature 2022 Abstract Prodigiosin is a tripyrrole red pigment that has been found to have antimicrobial, anticancer, and immunosuppressive properties. Experiments were conducted in this study to determine the best media types, temperature, and nitrogen and carbon sources for improving prodigiosin intracellular yield in Serratia marcescens. The nutrient broth (NB) medium at 30 °C yielded the highest level of prodigiosin (15 mg/L) when compared to peptone glycerol (PG), tryptone soy broth (TSB), Luria–Bertani broth (LBB), and glycerol beef broth (GBB). Lactose and peptone were found to be the most effective carbon and nitrogen sources, and when added to the nutrient broth, prodigiosin production increased to 18.67% (17.8 mg/L) and 22.67% (18.4 mg/L), respectively. To extract prodigiosin from Serratia marcescens biomass, the best ratio of organic solvent to water (pH 3) was 19: 1, and ethanol was found to be the best organic solvent to extract prodigiosin (15 mg/L) among DMSO, methanol, acetone, acetonitrile, and chloroform. To purify the prodigiosin, a column chromatography study was conducted, with Silica Gel 60–120 mesh as the stationary phase and a solvent mixture of chloroform, methanol, and acetone (4:2:3 v/v) as the mobile phase, yielding a purification yield of 65.39%. The absorption maximum of prodigiosin was found to be 535 nm in a UV–VIS study, and FTIR study confirmed that the functional group of prodigiosin is well matched with prior studies. The eluted fraction of the column contained 100% pure prodigiosin, which was validated by HPLC analysis. The LC–MS analysis also confirmed that prodigiosin was present in the eluted fraction of the column, with a peak at 324 m/z. When compared to the commercial pricing of prodigiosin on the worldwide market, modified media I (nutrient broth with 5% peanut), modified media II (nutrient broth with 5% sesame), and conventional media (nutrient broth) were determined to be economically feasible. The total saleable costs for 1 mg of prodigiosin obtained from modified media I, modified media II, and conventional media are 9229.41 INR (120.68 $), 14,502.66 INR (189.63 $), and 20,301.46 INR (265.45 $) respectively. The economic gain obtained from the modified media I, modified media II, and conventional media was found to be 362.27 $, 293.32 $, and 217.50 $ per mg of prodigiosin respectively. Column chromatography (dpeaa)DE-He213 Cost–benefit analysis (dpeaa)DE-He213 Prodigiosin (dpeaa)DE-He213 Extraction (dpeaa)DE-He213 Purification, characterization (dpeaa)DE-He213 Mondal, Abhijit aut Bandyopadhyay, Tarun Kanti aut Bhunia, Biswanath (orcid)0000-0003-1330-1606 aut Enthalten in Biomass Conversion and Biorefinery Berlin : Springer, 2011 14(2022), 3 vom: 06. Apr., Seite 4091-4110 (DE-627)645092843 (DE-600)2592298-1 2190-6823 nnns volume:14 year:2022 number:3 day:06 month:04 pages:4091-4110 https://dx.doi.org/10.1007/s13399-022-02639-2 lizenzpflichtig Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 14 2022 3 06 04 4091-4110 |
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Enthalten in Biomass Conversion and Biorefinery 14(2022), 3 vom: 06. Apr., Seite 4091-4110 volume:14 year:2022 number:3 day:06 month:04 pages:4091-4110 |
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Experiments were conducted in this study to determine the best media types, temperature, and nitrogen and carbon sources for improving prodigiosin intracellular yield in Serratia marcescens. The nutrient broth (NB) medium at 30 °C yielded the highest level of prodigiosin (15 mg/L) when compared to peptone glycerol (PG), tryptone soy broth (TSB), Luria–Bertani broth (LBB), and glycerol beef broth (GBB). Lactose and peptone were found to be the most effective carbon and nitrogen sources, and when added to the nutrient broth, prodigiosin production increased to 18.67% (17.8 mg/L) and 22.67% (18.4 mg/L), respectively. To extract prodigiosin from Serratia marcescens biomass, the best ratio of organic solvent to water (pH 3) was 19: 1, and ethanol was found to be the best organic solvent to extract prodigiosin (15 mg/L) among DMSO, methanol, acetone, acetonitrile, and chloroform. To purify the prodigiosin, a column chromatography study was conducted, with Silica Gel 60–120 mesh as the stationary phase and a solvent mixture of chloroform, methanol, and acetone (4:2:3 v/v) as the mobile phase, yielding a purification yield of 65.39%. The absorption maximum of prodigiosin was found to be 535 nm in a UV–VIS study, and FTIR study confirmed that the functional group of prodigiosin is well matched with prior studies. The eluted fraction of the column contained 100% pure prodigiosin, which was validated by HPLC analysis. The LC–MS analysis also confirmed that prodigiosin was present in the eluted fraction of the column, with a peak at 324 m/z. When compared to the commercial pricing of prodigiosin on the worldwide market, modified media I (nutrient broth with 5% peanut), modified media II (nutrient broth with 5% sesame), and conventional media (nutrient broth) were determined to be economically feasible. The total saleable costs for 1 mg of prodigiosin obtained from modified media I, modified media II, and conventional media are 9229.41 INR (120.68 $), 14,502.66 INR (189.63 $), and 20,301.46 INR (265.45 $) respectively. 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Paul, Tania |
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Paul, Tania misc Column chromatography misc Cost–benefit analysis misc Prodigiosin misc Extraction misc Purification, characterization Prodigiosin production and recovery from Serratia marcescens: process development and cost–benefit analysis |
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prodigiosin production and recovery from serratia marcescens: process development and cost–benefit analysis |
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Prodigiosin production and recovery from Serratia marcescens: process development and cost–benefit analysis |
| abstract |
Abstract Prodigiosin is a tripyrrole red pigment that has been found to have antimicrobial, anticancer, and immunosuppressive properties. Experiments were conducted in this study to determine the best media types, temperature, and nitrogen and carbon sources for improving prodigiosin intracellular yield in Serratia marcescens. The nutrient broth (NB) medium at 30 °C yielded the highest level of prodigiosin (15 mg/L) when compared to peptone glycerol (PG), tryptone soy broth (TSB), Luria–Bertani broth (LBB), and glycerol beef broth (GBB). Lactose and peptone were found to be the most effective carbon and nitrogen sources, and when added to the nutrient broth, prodigiosin production increased to 18.67% (17.8 mg/L) and 22.67% (18.4 mg/L), respectively. To extract prodigiosin from Serratia marcescens biomass, the best ratio of organic solvent to water (pH 3) was 19: 1, and ethanol was found to be the best organic solvent to extract prodigiosin (15 mg/L) among DMSO, methanol, acetone, acetonitrile, and chloroform. To purify the prodigiosin, a column chromatography study was conducted, with Silica Gel 60–120 mesh as the stationary phase and a solvent mixture of chloroform, methanol, and acetone (4:2:3 v/v) as the mobile phase, yielding a purification yield of 65.39%. The absorption maximum of prodigiosin was found to be 535 nm in a UV–VIS study, and FTIR study confirmed that the functional group of prodigiosin is well matched with prior studies. The eluted fraction of the column contained 100% pure prodigiosin, which was validated by HPLC analysis. The LC–MS analysis also confirmed that prodigiosin was present in the eluted fraction of the column, with a peak at 324 m/z. When compared to the commercial pricing of prodigiosin on the worldwide market, modified media I (nutrient broth with 5% peanut), modified media II (nutrient broth with 5% sesame), and conventional media (nutrient broth) were determined to be economically feasible. The total saleable costs for 1 mg of prodigiosin obtained from modified media I, modified media II, and conventional media are 9229.41 INR (120.68 $), 14,502.66 INR (189.63 $), and 20,301.46 INR (265.45 $) respectively. The economic gain obtained from the modified media I, modified media II, and conventional media was found to be 362.27 $, 293.32 $, and 217.50 $ per mg of prodigiosin respectively. © The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature 2022 |
| abstractGer |
Abstract Prodigiosin is a tripyrrole red pigment that has been found to have antimicrobial, anticancer, and immunosuppressive properties. Experiments were conducted in this study to determine the best media types, temperature, and nitrogen and carbon sources for improving prodigiosin intracellular yield in Serratia marcescens. The nutrient broth (NB) medium at 30 °C yielded the highest level of prodigiosin (15 mg/L) when compared to peptone glycerol (PG), tryptone soy broth (TSB), Luria–Bertani broth (LBB), and glycerol beef broth (GBB). Lactose and peptone were found to be the most effective carbon and nitrogen sources, and when added to the nutrient broth, prodigiosin production increased to 18.67% (17.8 mg/L) and 22.67% (18.4 mg/L), respectively. To extract prodigiosin from Serratia marcescens biomass, the best ratio of organic solvent to water (pH 3) was 19: 1, and ethanol was found to be the best organic solvent to extract prodigiosin (15 mg/L) among DMSO, methanol, acetone, acetonitrile, and chloroform. To purify the prodigiosin, a column chromatography study was conducted, with Silica Gel 60–120 mesh as the stationary phase and a solvent mixture of chloroform, methanol, and acetone (4:2:3 v/v) as the mobile phase, yielding a purification yield of 65.39%. The absorption maximum of prodigiosin was found to be 535 nm in a UV–VIS study, and FTIR study confirmed that the functional group of prodigiosin is well matched with prior studies. The eluted fraction of the column contained 100% pure prodigiosin, which was validated by HPLC analysis. The LC–MS analysis also confirmed that prodigiosin was present in the eluted fraction of the column, with a peak at 324 m/z. When compared to the commercial pricing of prodigiosin on the worldwide market, modified media I (nutrient broth with 5% peanut), modified media II (nutrient broth with 5% sesame), and conventional media (nutrient broth) were determined to be economically feasible. The total saleable costs for 1 mg of prodigiosin obtained from modified media I, modified media II, and conventional media are 9229.41 INR (120.68 $), 14,502.66 INR (189.63 $), and 20,301.46 INR (265.45 $) respectively. The economic gain obtained from the modified media I, modified media II, and conventional media was found to be 362.27 $, 293.32 $, and 217.50 $ per mg of prodigiosin respectively. © The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature 2022 |
| abstract_unstemmed |
Abstract Prodigiosin is a tripyrrole red pigment that has been found to have antimicrobial, anticancer, and immunosuppressive properties. Experiments were conducted in this study to determine the best media types, temperature, and nitrogen and carbon sources for improving prodigiosin intracellular yield in Serratia marcescens. The nutrient broth (NB) medium at 30 °C yielded the highest level of prodigiosin (15 mg/L) when compared to peptone glycerol (PG), tryptone soy broth (TSB), Luria–Bertani broth (LBB), and glycerol beef broth (GBB). Lactose and peptone were found to be the most effective carbon and nitrogen sources, and when added to the nutrient broth, prodigiosin production increased to 18.67% (17.8 mg/L) and 22.67% (18.4 mg/L), respectively. To extract prodigiosin from Serratia marcescens biomass, the best ratio of organic solvent to water (pH 3) was 19: 1, and ethanol was found to be the best organic solvent to extract prodigiosin (15 mg/L) among DMSO, methanol, acetone, acetonitrile, and chloroform. To purify the prodigiosin, a column chromatography study was conducted, with Silica Gel 60–120 mesh as the stationary phase and a solvent mixture of chloroform, methanol, and acetone (4:2:3 v/v) as the mobile phase, yielding a purification yield of 65.39%. The absorption maximum of prodigiosin was found to be 535 nm in a UV–VIS study, and FTIR study confirmed that the functional group of prodigiosin is well matched with prior studies. The eluted fraction of the column contained 100% pure prodigiosin, which was validated by HPLC analysis. The LC–MS analysis also confirmed that prodigiosin was present in the eluted fraction of the column, with a peak at 324 m/z. When compared to the commercial pricing of prodigiosin on the worldwide market, modified media I (nutrient broth with 5% peanut), modified media II (nutrient broth with 5% sesame), and conventional media (nutrient broth) were determined to be economically feasible. The total saleable costs for 1 mg of prodigiosin obtained from modified media I, modified media II, and conventional media are 9229.41 INR (120.68 $), 14,502.66 INR (189.63 $), and 20,301.46 INR (265.45 $) respectively. The economic gain obtained from the modified media I, modified media II, and conventional media was found to be 362.27 $, 293.32 $, and 217.50 $ per mg of prodigiosin respectively. © The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature 2022 |
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| title_short |
Prodigiosin production and recovery from Serratia marcescens: process development and cost–benefit analysis |
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Mondal, Abhijit Bandyopadhyay, Tarun Kanti Bhunia, Biswanath |
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| score |
7.3981867 |