Effects of matcha tea extract on cell viability and estrogen receptor-β expression on MCF-7 breast cancer cells

Purpose In the following work, we investigated the effect of matcha green tea extract (MTE) on MCF-7 breast cancer cell viability and estrogen receptor-beta expression (ERβ). Methods MCF-7 cells were stimulated with MTE at concentrations of 5 and 10 µg/ml. Cell viability was assessed using a water-s...
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Autor*in:	Keckstein, Simon [verfasserIn] Tilgener, Constantin Jeschke, Udo Hofmann, Simone Vilsmaier, Theresa Keilmann, Lucia Heidegger, Helene Kaltofen, Till Batz, Falk Mahner, Sven Schröder, Lennard

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2023

Schlagwörter:	Matcha tea extract MTE MCF-7 ERβ WST-1 PCR Western blot

Anmerkung:	© The Author(s) 2023

Übergeordnetes Werk:	Enthalten in: Archives of gynecology and obstetrics - Berlin : Springer, 1870, 309(2023), 4 vom: 22. Sept., Seite 1509-1514
Übergeordnetes Werk:	volume:309 ; year:2023 ; number:4 ; day:22 ; month:09 ; pages:1509-1514

Links:	Volltext

DOI / URN:	10.1007/s00404-023-07209-z

Katalog-ID:	SPR054883059

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100	1		\|a Keckstein, Simon \|e verfasserin \|4 aut
245	1	0	\|a Effects of matcha tea extract on cell viability and estrogen receptor-β expression on MCF-7 breast cancer cells
264		1	\|c 2023
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520			\|a Purpose In the following work, we investigated the effect of matcha green tea extract (MTE) on MCF-7 breast cancer cell viability and estrogen receptor-beta expression (ERβ). Methods MCF-7 cells were stimulated with MTE at concentrations of 5 and 10 µg/ml. Cell viability was assessed using a water-soluble tetrazolium assay (WST-1 assay) after an incubation time of 72 h. ERβ was quantified at gene level by real-time polymerase chain reaction (PCR). A western blot (WB) was carried out for the qualitative assessment of the expression behavior of on a protein level. Results The WST-1 test showed a significant inhibition of viability in MFC-7 cells after 72 h at 10 µg/ml. The WB demonstrated a significant quantitative decrease of ERβ at protein level with MTE concentrations of 10 µg/ml. In contrast, the PCR did not result in significant downregulation of ERβ. Conclusion MTE decreases the cell viability of MCF-7 cells and furthermore leads to a decrease of ERβ at protein level.
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650		4	\|a MTE \|7 (dpeaa)DE-He213
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650		4	\|a ERβ \|7 (dpeaa)DE-He213
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700	1		\|a Tilgener, Constantin \|4 aut
700	1		\|a Jeschke, Udo \|0 (orcid)0000-0003-2623-3235 \|4 aut
700	1		\|a Hofmann, Simone \|4 aut
700	1		\|a Vilsmaier, Theresa \|4 aut
700	1		\|a Keilmann, Lucia \|4 aut
700	1		\|a Heidegger, Helene \|4 aut
700	1		\|a Kaltofen, Till \|4 aut
700	1		\|a Batz, Falk \|4 aut
700	1		\|a Mahner, Sven \|4 aut
700	1		\|a Schröder, Lennard \|4 aut
773	0	8	\|i Enthalten in \|t Archives of gynecology and obstetrics \|d Berlin : Springer, 1870 \|g 309(2023), 4 vom: 22. Sept., Seite 1509-1514 \|w (DE-627)253390060 \|w (DE-600)1458450-5 \|x 1432-0711 \|7 nnns
773	1	8	\|g volume:309 \|g year:2023 \|g number:4 \|g day:22 \|g month:09 \|g pages:1509-1514
856	4	0	\|u https://dx.doi.org/10.1007/s00404-023-07209-z \|z kostenfrei \|3 Volltext
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912			\|a GBV_ILN_101
912			\|a GBV_ILN_105
912			\|a GBV_ILN_110
912			\|a GBV_ILN_120
912			\|a GBV_ILN_138
912			\|a GBV_ILN_150
912			\|a GBV_ILN_151
912			\|a GBV_ILN_152
912			\|a GBV_ILN_161
912			\|a GBV_ILN_170
912			\|a GBV_ILN_171
912			\|a GBV_ILN_187
912			\|a GBV_ILN_213
912			\|a GBV_ILN_224
912			\|a GBV_ILN_230
912			\|a GBV_ILN_250
912			\|a GBV_ILN_267
912			\|a GBV_ILN_281
912			\|a GBV_ILN_285
912			\|a GBV_ILN_293
912			\|a GBV_ILN_370
912			\|a GBV_ILN_602
912			\|a GBV_ILN_636
912			\|a GBV_ILN_702
912			\|a GBV_ILN_711
912			\|a GBV_ILN_2001
912			\|a GBV_ILN_2003
912			\|a GBV_ILN_2004
912			\|a GBV_ILN_2005
912			\|a GBV_ILN_2006
912			\|a GBV_ILN_2007
912			\|a GBV_ILN_2008
912			\|a GBV_ILN_2009
912			\|a GBV_ILN_2010
912			\|a GBV_ILN_2011
912			\|a GBV_ILN_2014
912			\|a GBV_ILN_2015
912			\|a GBV_ILN_2018
912			\|a GBV_ILN_2020
912			\|a GBV_ILN_2021
912			\|a GBV_ILN_2025
912			\|a GBV_ILN_2026
912			\|a GBV_ILN_2027
912			\|a GBV_ILN_2031
912			\|a GBV_ILN_2034
912			\|a GBV_ILN_2037
912			\|a GBV_ILN_2038
912			\|a GBV_ILN_2039
912			\|a GBV_ILN_2044
912			\|a GBV_ILN_2048
912			\|a GBV_ILN_2049
912			\|a GBV_ILN_2050
912			\|a GBV_ILN_2055
912			\|a GBV_ILN_2056
912			\|a GBV_ILN_2057
912			\|a GBV_ILN_2059
912			\|a GBV_ILN_2061
912			\|a GBV_ILN_2064
912			\|a GBV_ILN_2065
912			\|a GBV_ILN_2068
912			\|a GBV_ILN_2088
912			\|a GBV_ILN_2093
912			\|a GBV_ILN_2106
912			\|a GBV_ILN_2107
912			\|a GBV_ILN_2108
912			\|a GBV_ILN_2110
912			\|a GBV_ILN_2111
912			\|a GBV_ILN_2112
912			\|a GBV_ILN_2113
912			\|a GBV_ILN_2118
912			\|a GBV_ILN_2122
912			\|a GBV_ILN_2129
912			\|a GBV_ILN_2143
912			\|a GBV_ILN_2144
912			\|a GBV_ILN_2147
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912			\|a GBV_ILN_2336
912			\|a GBV_ILN_2446
912			\|a GBV_ILN_2470
912			\|a GBV_ILN_2472
912			\|a GBV_ILN_2507
912			\|a GBV_ILN_2522
912			\|a GBV_ILN_2548
912			\|a GBV_ILN_4035
912			\|a GBV_ILN_4037
912			\|a GBV_ILN_4046
912			\|a GBV_ILN_4112
912			\|a GBV_ILN_4125
912			\|a GBV_ILN_4126
912			\|a GBV_ILN_4242
912			\|a GBV_ILN_4246
912			\|a GBV_ILN_4249
912			\|a GBV_ILN_4251
912			\|a GBV_ILN_4277
912			\|a GBV_ILN_4305
912			\|a GBV_ILN_4306
912			\|a GBV_ILN_4307
912			\|a GBV_ILN_4313
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912			\|a GBV_ILN_4323
912			\|a GBV_ILN_4324
912			\|a GBV_ILN_4325
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912			\|a GBV_ILN_4328
912			\|a GBV_ILN_4333
912			\|a GBV_ILN_4334
912			\|a GBV_ILN_4335
912			\|a GBV_ILN_4336
912			\|a GBV_ILN_4338
912			\|a GBV_ILN_4393
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951			\|a AR
952			\|d 309 \|j 2023 \|e 4 \|b 22 \|c 09 \|h 1509-1514

Indexfelder

author_variant	s k sk c t ct u j uj s h sh t v tv l k lk h h hh t k tk f b fb s m sm l s ls
matchkey_str	article:14320711:2023----::fetomthtaxrcoclvaiiynetoercpoepeso
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publishDate	2023
allfields	10.1007/s00404-023-07209-z doi (DE-627)SPR054883059 (SPR)s00404-023-07209-z-e DE-627 ger DE-627 rakwb eng Keckstein, Simon verfasserin aut Effects of matcha tea extract on cell viability and estrogen receptor-β expression on MCF-7 breast cancer cells 2023 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s) 2023 Purpose In the following work, we investigated the effect of matcha green tea extract (MTE) on MCF-7 breast cancer cell viability and estrogen receptor-beta expression (ERβ). Methods MCF-7 cells were stimulated with MTE at concentrations of 5 and 10 µg/ml. Cell viability was assessed using a water-soluble tetrazolium assay (WST-1 assay) after an incubation time of 72 h. ERβ was quantified at gene level by real-time polymerase chain reaction (PCR). A western blot (WB) was carried out for the qualitative assessment of the expression behavior of on a protein level. Results The WST-1 test showed a significant inhibition of viability in MFC-7 cells after 72 h at 10 µg/ml. The WB demonstrated a significant quantitative decrease of ERβ at protein level with MTE concentrations of 10 µg/ml. In contrast, the PCR did not result in significant downregulation of ERβ. Conclusion MTE decreases the cell viability of MCF-7 cells and furthermore leads to a decrease of ERβ at protein level. Matcha tea extract (dpeaa)DE-He213 MTE (dpeaa)DE-He213 MCF-7 (dpeaa)DE-He213 ERβ (dpeaa)DE-He213 WST-1 (dpeaa)DE-He213 PCR (dpeaa)DE-He213 Western blot (dpeaa)DE-He213 Tilgener, Constantin aut Jeschke, Udo (orcid)0000-0003-2623-3235 aut Hofmann, Simone aut Vilsmaier, Theresa aut Keilmann, Lucia aut Heidegger, Helene aut Kaltofen, Till aut Batz, Falk aut Mahner, Sven aut Schröder, Lennard aut Enthalten in Archives of gynecology and obstetrics Berlin : Springer, 1870 309(2023), 4 vom: 22. Sept., Seite 1509-1514 (DE-627)253390060 (DE-600)1458450-5 1432-0711 nnns volume:309 year:2023 number:4 day:22 month:09 pages:1509-1514 https://dx.doi.org/10.1007/s00404-023-07209-z kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_267 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_711 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2018 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4277 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 309 2023 4 22 09 1509-1514
spelling	10.1007/s00404-023-07209-z doi (DE-627)SPR054883059 (SPR)s00404-023-07209-z-e DE-627 ger DE-627 rakwb eng Keckstein, Simon verfasserin aut Effects of matcha tea extract on cell viability and estrogen receptor-β expression on MCF-7 breast cancer cells 2023 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s) 2023 Purpose In the following work, we investigated the effect of matcha green tea extract (MTE) on MCF-7 breast cancer cell viability and estrogen receptor-beta expression (ERβ). Methods MCF-7 cells were stimulated with MTE at concentrations of 5 and 10 µg/ml. Cell viability was assessed using a water-soluble tetrazolium assay (WST-1 assay) after an incubation time of 72 h. ERβ was quantified at gene level by real-time polymerase chain reaction (PCR). A western blot (WB) was carried out for the qualitative assessment of the expression behavior of on a protein level. Results The WST-1 test showed a significant inhibition of viability in MFC-7 cells after 72 h at 10 µg/ml. The WB demonstrated a significant quantitative decrease of ERβ at protein level with MTE concentrations of 10 µg/ml. In contrast, the PCR did not result in significant downregulation of ERβ. Conclusion MTE decreases the cell viability of MCF-7 cells and furthermore leads to a decrease of ERβ at protein level. Matcha tea extract (dpeaa)DE-He213 MTE (dpeaa)DE-He213 MCF-7 (dpeaa)DE-He213 ERβ (dpeaa)DE-He213 WST-1 (dpeaa)DE-He213 PCR (dpeaa)DE-He213 Western blot (dpeaa)DE-He213 Tilgener, Constantin aut Jeschke, Udo (orcid)0000-0003-2623-3235 aut Hofmann, Simone aut Vilsmaier, Theresa aut Keilmann, Lucia aut Heidegger, Helene aut Kaltofen, Till aut Batz, Falk aut Mahner, Sven aut Schröder, Lennard aut Enthalten in Archives of gynecology and obstetrics Berlin : Springer, 1870 309(2023), 4 vom: 22. Sept., Seite 1509-1514 (DE-627)253390060 (DE-600)1458450-5 1432-0711 nnns volume:309 year:2023 number:4 day:22 month:09 pages:1509-1514 https://dx.doi.org/10.1007/s00404-023-07209-z kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_267 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_711 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2018 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4277 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 309 2023 4 22 09 1509-1514
allfields_unstemmed	10.1007/s00404-023-07209-z doi (DE-627)SPR054883059 (SPR)s00404-023-07209-z-e DE-627 ger DE-627 rakwb eng Keckstein, Simon verfasserin aut Effects of matcha tea extract on cell viability and estrogen receptor-β expression on MCF-7 breast cancer cells 2023 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s) 2023 Purpose In the following work, we investigated the effect of matcha green tea extract (MTE) on MCF-7 breast cancer cell viability and estrogen receptor-beta expression (ERβ). Methods MCF-7 cells were stimulated with MTE at concentrations of 5 and 10 µg/ml. Cell viability was assessed using a water-soluble tetrazolium assay (WST-1 assay) after an incubation time of 72 h. ERβ was quantified at gene level by real-time polymerase chain reaction (PCR). A western blot (WB) was carried out for the qualitative assessment of the expression behavior of on a protein level. Results The WST-1 test showed a significant inhibition of viability in MFC-7 cells after 72 h at 10 µg/ml. The WB demonstrated a significant quantitative decrease of ERβ at protein level with MTE concentrations of 10 µg/ml. In contrast, the PCR did not result in significant downregulation of ERβ. Conclusion MTE decreases the cell viability of MCF-7 cells and furthermore leads to a decrease of ERβ at protein level. Matcha tea extract (dpeaa)DE-He213 MTE (dpeaa)DE-He213 MCF-7 (dpeaa)DE-He213 ERβ (dpeaa)DE-He213 WST-1 (dpeaa)DE-He213 PCR (dpeaa)DE-He213 Western blot (dpeaa)DE-He213 Tilgener, Constantin aut Jeschke, Udo (orcid)0000-0003-2623-3235 aut Hofmann, Simone aut Vilsmaier, Theresa aut Keilmann, Lucia aut Heidegger, Helene aut Kaltofen, Till aut Batz, Falk aut Mahner, Sven aut Schröder, Lennard aut Enthalten in Archives of gynecology and obstetrics Berlin : Springer, 1870 309(2023), 4 vom: 22. Sept., Seite 1509-1514 (DE-627)253390060 (DE-600)1458450-5 1432-0711 nnns volume:309 year:2023 number:4 day:22 month:09 pages:1509-1514 https://dx.doi.org/10.1007/s00404-023-07209-z kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_267 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_711 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2018 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4277 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 309 2023 4 22 09 1509-1514
allfieldsGer	10.1007/s00404-023-07209-z doi (DE-627)SPR054883059 (SPR)s00404-023-07209-z-e DE-627 ger DE-627 rakwb eng Keckstein, Simon verfasserin aut Effects of matcha tea extract on cell viability and estrogen receptor-β expression on MCF-7 breast cancer cells 2023 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s) 2023 Purpose In the following work, we investigated the effect of matcha green tea extract (MTE) on MCF-7 breast cancer cell viability and estrogen receptor-beta expression (ERβ). Methods MCF-7 cells were stimulated with MTE at concentrations of 5 and 10 µg/ml. Cell viability was assessed using a water-soluble tetrazolium assay (WST-1 assay) after an incubation time of 72 h. ERβ was quantified at gene level by real-time polymerase chain reaction (PCR). A western blot (WB) was carried out for the qualitative assessment of the expression behavior of on a protein level. Results The WST-1 test showed a significant inhibition of viability in MFC-7 cells after 72 h at 10 µg/ml. The WB demonstrated a significant quantitative decrease of ERβ at protein level with MTE concentrations of 10 µg/ml. In contrast, the PCR did not result in significant downregulation of ERβ. Conclusion MTE decreases the cell viability of MCF-7 cells and furthermore leads to a decrease of ERβ at protein level. Matcha tea extract (dpeaa)DE-He213 MTE (dpeaa)DE-He213 MCF-7 (dpeaa)DE-He213 ERβ (dpeaa)DE-He213 WST-1 (dpeaa)DE-He213 PCR (dpeaa)DE-He213 Western blot (dpeaa)DE-He213 Tilgener, Constantin aut Jeschke, Udo (orcid)0000-0003-2623-3235 aut Hofmann, Simone aut Vilsmaier, Theresa aut Keilmann, Lucia aut Heidegger, Helene aut Kaltofen, Till aut Batz, Falk aut Mahner, Sven aut Schröder, Lennard aut Enthalten in Archives of gynecology and obstetrics Berlin : Springer, 1870 309(2023), 4 vom: 22. Sept., Seite 1509-1514 (DE-627)253390060 (DE-600)1458450-5 1432-0711 nnns volume:309 year:2023 number:4 day:22 month:09 pages:1509-1514 https://dx.doi.org/10.1007/s00404-023-07209-z kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_267 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_711 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2018 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4277 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 309 2023 4 22 09 1509-1514
allfieldsSound	10.1007/s00404-023-07209-z doi (DE-627)SPR054883059 (SPR)s00404-023-07209-z-e DE-627 ger DE-627 rakwb eng Keckstein, Simon verfasserin aut Effects of matcha tea extract on cell viability and estrogen receptor-β expression on MCF-7 breast cancer cells 2023 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s) 2023 Purpose In the following work, we investigated the effect of matcha green tea extract (MTE) on MCF-7 breast cancer cell viability and estrogen receptor-beta expression (ERβ). Methods MCF-7 cells were stimulated with MTE at concentrations of 5 and 10 µg/ml. Cell viability was assessed using a water-soluble tetrazolium assay (WST-1 assay) after an incubation time of 72 h. ERβ was quantified at gene level by real-time polymerase chain reaction (PCR). A western blot (WB) was carried out for the qualitative assessment of the expression behavior of on a protein level. Results The WST-1 test showed a significant inhibition of viability in MFC-7 cells after 72 h at 10 µg/ml. The WB demonstrated a significant quantitative decrease of ERβ at protein level with MTE concentrations of 10 µg/ml. In contrast, the PCR did not result in significant downregulation of ERβ. Conclusion MTE decreases the cell viability of MCF-7 cells and furthermore leads to a decrease of ERβ at protein level. Matcha tea extract (dpeaa)DE-He213 MTE (dpeaa)DE-He213 MCF-7 (dpeaa)DE-He213 ERβ (dpeaa)DE-He213 WST-1 (dpeaa)DE-He213 PCR (dpeaa)DE-He213 Western blot (dpeaa)DE-He213 Tilgener, Constantin aut Jeschke, Udo (orcid)0000-0003-2623-3235 aut Hofmann, Simone aut Vilsmaier, Theresa aut Keilmann, Lucia aut Heidegger, Helene aut Kaltofen, Till aut Batz, Falk aut Mahner, Sven aut Schröder, Lennard aut Enthalten in Archives of gynecology and obstetrics Berlin : Springer, 1870 309(2023), 4 vom: 22. Sept., Seite 1509-1514 (DE-627)253390060 (DE-600)1458450-5 1432-0711 nnns volume:309 year:2023 number:4 day:22 month:09 pages:1509-1514 https://dx.doi.org/10.1007/s00404-023-07209-z kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_267 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_711 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2018 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4277 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 309 2023 4 22 09 1509-1514
language	English
source	Enthalten in Archives of gynecology and obstetrics 309(2023), 4 vom: 22. Sept., Seite 1509-1514 volume:309 year:2023 number:4 day:22 month:09 pages:1509-1514
sourceStr	Enthalten in Archives of gynecology and obstetrics 309(2023), 4 vom: 22. Sept., Seite 1509-1514 volume:309 year:2023 number:4 day:22 month:09 pages:1509-1514
format_phy_str_mv	Article
institution	findex.gbv.de
topic_facet	Matcha tea extract MTE MCF-7 ERβ WST-1 PCR Western blot
isfreeaccess_bool	true
container_title	Archives of gynecology and obstetrics
authorswithroles_txt_mv	Keckstein, Simon @@aut@@ Tilgener, Constantin @@aut@@ Jeschke, Udo @@aut@@ Hofmann, Simone @@aut@@ Vilsmaier, Theresa @@aut@@ Keilmann, Lucia @@aut@@ Heidegger, Helene @@aut@@ Kaltofen, Till @@aut@@ Batz, Falk @@aut@@ Mahner, Sven @@aut@@ Schröder, Lennard @@aut@@
publishDateDaySort_date	2023-09-22T00:00:00Z
hierarchy_top_id	253390060
id	SPR054883059
language_de	englisch
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Methods MCF-7 cells were stimulated with MTE at concentrations of 5 and 10 µg/ml. Cell viability was assessed using a water-soluble tetrazolium assay (WST-1 assay) after an incubation time of 72 h. ERβ was quantified at gene level by real-time polymerase chain reaction (PCR). A western blot (WB) was carried out for the qualitative assessment of the expression behavior of on a protein level. Results The WST-1 test showed a significant inhibition of viability in MFC-7 cells after 72 h at 10 µg/ml. The WB demonstrated a significant quantitative decrease of ERβ at protein level with MTE concentrations of 10 µg/ml. In contrast, the PCR did not result in significant downregulation of ERβ. 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author	Keckstein, Simon
spellingShingle	Keckstein, Simon misc Matcha tea extract misc MTE misc MCF-7 misc ERβ misc WST-1 misc PCR misc Western blot Effects of matcha tea extract on cell viability and estrogen receptor-β expression on MCF-7 breast cancer cells
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topic_title	Effects of matcha tea extract on cell viability and estrogen receptor-β expression on MCF-7 breast cancer cells Matcha tea extract (dpeaa)DE-He213 MTE (dpeaa)DE-He213 MCF-7 (dpeaa)DE-He213 ERβ (dpeaa)DE-He213 WST-1 (dpeaa)DE-He213 PCR (dpeaa)DE-He213 Western blot (dpeaa)DE-He213
topic	misc Matcha tea extract misc MTE misc MCF-7 misc ERβ misc WST-1 misc PCR misc Western blot
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title	Effects of matcha tea extract on cell viability and estrogen receptor-β expression on MCF-7 breast cancer cells
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title_full	Effects of matcha tea extract on cell viability and estrogen receptor-β expression on MCF-7 breast cancer cells
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author_browse	Keckstein, Simon Tilgener, Constantin Jeschke, Udo Hofmann, Simone Vilsmaier, Theresa Keilmann, Lucia Heidegger, Helene Kaltofen, Till Batz, Falk Mahner, Sven Schröder, Lennard
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title_sort	effects of matcha tea extract on cell viability and estrogen receptor-β expression on mcf-7 breast cancer cells
title_auth	Effects of matcha tea extract on cell viability and estrogen receptor-β expression on MCF-7 breast cancer cells
abstract	Purpose In the following work, we investigated the effect of matcha green tea extract (MTE) on MCF-7 breast cancer cell viability and estrogen receptor-beta expression (ERβ). Methods MCF-7 cells were stimulated with MTE at concentrations of 5 and 10 µg/ml. Cell viability was assessed using a water-soluble tetrazolium assay (WST-1 assay) after an incubation time of 72 h. ERβ was quantified at gene level by real-time polymerase chain reaction (PCR). A western blot (WB) was carried out for the qualitative assessment of the expression behavior of on a protein level. Results The WST-1 test showed a significant inhibition of viability in MFC-7 cells after 72 h at 10 µg/ml. The WB demonstrated a significant quantitative decrease of ERβ at protein level with MTE concentrations of 10 µg/ml. In contrast, the PCR did not result in significant downregulation of ERβ. Conclusion MTE decreases the cell viability of MCF-7 cells and furthermore leads to a decrease of ERβ at protein level. © The Author(s) 2023
abstractGer	Purpose In the following work, we investigated the effect of matcha green tea extract (MTE) on MCF-7 breast cancer cell viability and estrogen receptor-beta expression (ERβ). Methods MCF-7 cells were stimulated with MTE at concentrations of 5 and 10 µg/ml. Cell viability was assessed using a water-soluble tetrazolium assay (WST-1 assay) after an incubation time of 72 h. ERβ was quantified at gene level by real-time polymerase chain reaction (PCR). A western blot (WB) was carried out for the qualitative assessment of the expression behavior of on a protein level. Results The WST-1 test showed a significant inhibition of viability in MFC-7 cells after 72 h at 10 µg/ml. The WB demonstrated a significant quantitative decrease of ERβ at protein level with MTE concentrations of 10 µg/ml. In contrast, the PCR did not result in significant downregulation of ERβ. Conclusion MTE decreases the cell viability of MCF-7 cells and furthermore leads to a decrease of ERβ at protein level. © The Author(s) 2023
abstract_unstemmed	Purpose In the following work, we investigated the effect of matcha green tea extract (MTE) on MCF-7 breast cancer cell viability and estrogen receptor-beta expression (ERβ). Methods MCF-7 cells were stimulated with MTE at concentrations of 5 and 10 µg/ml. Cell viability was assessed using a water-soluble tetrazolium assay (WST-1 assay) after an incubation time of 72 h. ERβ was quantified at gene level by real-time polymerase chain reaction (PCR). A western blot (WB) was carried out for the qualitative assessment of the expression behavior of on a protein level. Results The WST-1 test showed a significant inhibition of viability in MFC-7 cells after 72 h at 10 µg/ml. The WB demonstrated a significant quantitative decrease of ERβ at protein level with MTE concentrations of 10 µg/ml. In contrast, the PCR did not result in significant downregulation of ERβ. Conclusion MTE decreases the cell viability of MCF-7 cells and furthermore leads to a decrease of ERβ at protein level. © The Author(s) 2023
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title_short	Effects of matcha tea extract on cell viability and estrogen receptor-β expression on MCF-7 breast cancer cells
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up_date	2024-07-04T03:22:31.467Z
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Methods MCF-7 cells were stimulated with MTE at concentrations of 5 and 10 µg/ml. Cell viability was assessed using a water-soluble tetrazolium assay (WST-1 assay) after an incubation time of 72 h. ERβ was quantified at gene level by real-time polymerase chain reaction (PCR). A western blot (WB) was carried out for the qualitative assessment of the expression behavior of on a protein level. Results The WST-1 test showed a significant inhibition of viability in MFC-7 cells after 72 h at 10 µg/ml. The WB demonstrated a significant quantitative decrease of ERβ at protein level with MTE concentrations of 10 µg/ml. In contrast, the PCR did not result in significant downregulation of ERβ. 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Effects of matcha tea extract on cell viability and estrogen receptor-β expression on MCF-7 breast cancer cells

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