Evaluating oleaginous yeasts for enhanced microbial lipid production using sweetwater as a sustainable feedstock

Background Yeasts exhibit promising potential for the microbial conversion of crude glycerol, owing to their versatility in delivering a wide range of value-added products, particularly lipids. Sweetwater, a methanol-free by-product of the fat splitting process, has emerged as a promising alternativ...
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Autor*in:	Keita, Valériane Malika [verfasserIn] Lee, Yi Qing Lakshmanan, Meiyappan Ow, Dave Siak-Wei Staniland, Paul Staniland, Jessica Savill, Ian Tee, Kang Lan Wong, Tuck Seng Lee, Dong-Yup

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2024

Schlagwörter:	Oleaginous yeasts Microbial oil Microbial cell factories Sweetwater Comparative omics

Anmerkung:	© The Author(s) 2024

Übergeordnetes Werk:	Enthalten in: Microbial cell factories - London : Biomed Central, 2002, 23(2024), 1 vom: 24. Feb.
Übergeordnetes Werk:	volume:23 ; year:2024 ; number:1 ; day:24 ; month:02

Links:	Volltext

DOI / URN:	10.1186/s12934-024-02336-x

Katalog-ID:	SPR054887860

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520			\|a Background Yeasts exhibit promising potential for the microbial conversion of crude glycerol, owing to their versatility in delivering a wide range of value-added products, particularly lipids. Sweetwater, a methanol-free by-product of the fat splitting process, has emerged as a promising alternative feedstock for the microbial utilization of crude glycerol. To further optimize sweetwater utilization, we compared the growth and lipid production capabilities of 21 oleaginous yeast strains under different conditions with various glycerol concentrations, sweetwater types and pH. Results We found that nutrient limitation and the unique carbon composition of sweetwater boosted significant lipid accumulation in several strains, in particular Rhodosporidium toruloides NRRL Y-6987. Subsequently, to decipher the underlying mechanism, the transcriptomic changes of R. toruloides NRRL Y-6987 were further analyzed, indicating potential sugars and oligopeptides in sweetwater supporting growth and lipid accumulation as well as exogenous fatty acid uptake leading to the enhanced lipid accumulation. Conclusion Our comparative study successfully demonstrated sweetwater as a cost-effective feedstock while identifying R. toluroides NRRL Y-6987 as a highly promising microbial oil producer. Furthermore, we also suggested potential sweetwater type and strain engineering targets that could potentially enhance microbial lipid production. Graphical abstract
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allfields	10.1186/s12934-024-02336-x doi (DE-627)SPR054887860 (SPR)s12934-024-02336-x-e DE-627 ger DE-627 rakwb eng Keita, Valériane Malika verfasserin aut Evaluating oleaginous yeasts for enhanced microbial lipid production using sweetwater as a sustainable feedstock 2024 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s) 2024 Background Yeasts exhibit promising potential for the microbial conversion of crude glycerol, owing to their versatility in delivering a wide range of value-added products, particularly lipids. Sweetwater, a methanol-free by-product of the fat splitting process, has emerged as a promising alternative feedstock for the microbial utilization of crude glycerol. To further optimize sweetwater utilization, we compared the growth and lipid production capabilities of 21 oleaginous yeast strains under different conditions with various glycerol concentrations, sweetwater types and pH. Results We found that nutrient limitation and the unique carbon composition of sweetwater boosted significant lipid accumulation in several strains, in particular Rhodosporidium toruloides NRRL Y-6987. Subsequently, to decipher the underlying mechanism, the transcriptomic changes of R. toruloides NRRL Y-6987 were further analyzed, indicating potential sugars and oligopeptides in sweetwater supporting growth and lipid accumulation as well as exogenous fatty acid uptake leading to the enhanced lipid accumulation. Conclusion Our comparative study successfully demonstrated sweetwater as a cost-effective feedstock while identifying R. toluroides NRRL Y-6987 as a highly promising microbial oil producer. Furthermore, we also suggested potential sweetwater type and strain engineering targets that could potentially enhance microbial lipid production. Graphical abstract Oleaginous yeasts (dpeaa)DE-He213 Microbial oil (dpeaa)DE-He213 Microbial cell factories (dpeaa)DE-He213 Sweetwater (dpeaa)DE-He213 Comparative omics (dpeaa)DE-He213 Lee, Yi Qing (orcid)0000-0002-8970-0729 aut Lakshmanan, Meiyappan (orcid)0000-0003-2356-3458 aut Ow, Dave Siak-Wei (orcid)0000-0002-0436-1896 aut Staniland, Paul aut Staniland, Jessica aut Savill, Ian aut Tee, Kang Lan (orcid)0000-0001-9458-733X aut Wong, Tuck Seng (orcid)0000-0001-7689-9057 aut Lee, Dong-Yup (orcid)0000-0003-0901-708X aut Enthalten in Microbial cell factories London : Biomed Central, 2002 23(2024), 1 vom: 24. Feb. (DE-627)355987651 (DE-600)2091377-1 1475-2859 nnns volume:23 year:2024 number:1 day:24 month:02 https://dx.doi.org/10.1186/s12934-024-02336-x kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 23 2024 1 24 02
spelling	10.1186/s12934-024-02336-x doi (DE-627)SPR054887860 (SPR)s12934-024-02336-x-e DE-627 ger DE-627 rakwb eng Keita, Valériane Malika verfasserin aut Evaluating oleaginous yeasts for enhanced microbial lipid production using sweetwater as a sustainable feedstock 2024 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s) 2024 Background Yeasts exhibit promising potential for the microbial conversion of crude glycerol, owing to their versatility in delivering a wide range of value-added products, particularly lipids. Sweetwater, a methanol-free by-product of the fat splitting process, has emerged as a promising alternative feedstock for the microbial utilization of crude glycerol. To further optimize sweetwater utilization, we compared the growth and lipid production capabilities of 21 oleaginous yeast strains under different conditions with various glycerol concentrations, sweetwater types and pH. Results We found that nutrient limitation and the unique carbon composition of sweetwater boosted significant lipid accumulation in several strains, in particular Rhodosporidium toruloides NRRL Y-6987. Subsequently, to decipher the underlying mechanism, the transcriptomic changes of R. toruloides NRRL Y-6987 were further analyzed, indicating potential sugars and oligopeptides in sweetwater supporting growth and lipid accumulation as well as exogenous fatty acid uptake leading to the enhanced lipid accumulation. Conclusion Our comparative study successfully demonstrated sweetwater as a cost-effective feedstock while identifying R. toluroides NRRL Y-6987 as a highly promising microbial oil producer. Furthermore, we also suggested potential sweetwater type and strain engineering targets that could potentially enhance microbial lipid production. Graphical abstract Oleaginous yeasts (dpeaa)DE-He213 Microbial oil (dpeaa)DE-He213 Microbial cell factories (dpeaa)DE-He213 Sweetwater (dpeaa)DE-He213 Comparative omics (dpeaa)DE-He213 Lee, Yi Qing (orcid)0000-0002-8970-0729 aut Lakshmanan, Meiyappan (orcid)0000-0003-2356-3458 aut Ow, Dave Siak-Wei (orcid)0000-0002-0436-1896 aut Staniland, Paul aut Staniland, Jessica aut Savill, Ian aut Tee, Kang Lan (orcid)0000-0001-9458-733X aut Wong, Tuck Seng (orcid)0000-0001-7689-9057 aut Lee, Dong-Yup (orcid)0000-0003-0901-708X aut Enthalten in Microbial cell factories London : Biomed Central, 2002 23(2024), 1 vom: 24. Feb. (DE-627)355987651 (DE-600)2091377-1 1475-2859 nnns volume:23 year:2024 number:1 day:24 month:02 https://dx.doi.org/10.1186/s12934-024-02336-x kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 23 2024 1 24 02
allfields_unstemmed	10.1186/s12934-024-02336-x doi (DE-627)SPR054887860 (SPR)s12934-024-02336-x-e DE-627 ger DE-627 rakwb eng Keita, Valériane Malika verfasserin aut Evaluating oleaginous yeasts for enhanced microbial lipid production using sweetwater as a sustainable feedstock 2024 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s) 2024 Background Yeasts exhibit promising potential for the microbial conversion of crude glycerol, owing to their versatility in delivering a wide range of value-added products, particularly lipids. Sweetwater, a methanol-free by-product of the fat splitting process, has emerged as a promising alternative feedstock for the microbial utilization of crude glycerol. To further optimize sweetwater utilization, we compared the growth and lipid production capabilities of 21 oleaginous yeast strains under different conditions with various glycerol concentrations, sweetwater types and pH. Results We found that nutrient limitation and the unique carbon composition of sweetwater boosted significant lipid accumulation in several strains, in particular Rhodosporidium toruloides NRRL Y-6987. Subsequently, to decipher the underlying mechanism, the transcriptomic changes of R. toruloides NRRL Y-6987 were further analyzed, indicating potential sugars and oligopeptides in sweetwater supporting growth and lipid accumulation as well as exogenous fatty acid uptake leading to the enhanced lipid accumulation. Conclusion Our comparative study successfully demonstrated sweetwater as a cost-effective feedstock while identifying R. toluroides NRRL Y-6987 as a highly promising microbial oil producer. Furthermore, we also suggested potential sweetwater type and strain engineering targets that could potentially enhance microbial lipid production. Graphical abstract Oleaginous yeasts (dpeaa)DE-He213 Microbial oil (dpeaa)DE-He213 Microbial cell factories (dpeaa)DE-He213 Sweetwater (dpeaa)DE-He213 Comparative omics (dpeaa)DE-He213 Lee, Yi Qing (orcid)0000-0002-8970-0729 aut Lakshmanan, Meiyappan (orcid)0000-0003-2356-3458 aut Ow, Dave Siak-Wei (orcid)0000-0002-0436-1896 aut Staniland, Paul aut Staniland, Jessica aut Savill, Ian aut Tee, Kang Lan (orcid)0000-0001-9458-733X aut Wong, Tuck Seng (orcid)0000-0001-7689-9057 aut Lee, Dong-Yup (orcid)0000-0003-0901-708X aut Enthalten in Microbial cell factories London : Biomed Central, 2002 23(2024), 1 vom: 24. Feb. (DE-627)355987651 (DE-600)2091377-1 1475-2859 nnns volume:23 year:2024 number:1 day:24 month:02 https://dx.doi.org/10.1186/s12934-024-02336-x kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 23 2024 1 24 02
allfieldsGer	10.1186/s12934-024-02336-x doi (DE-627)SPR054887860 (SPR)s12934-024-02336-x-e DE-627 ger DE-627 rakwb eng Keita, Valériane Malika verfasserin aut Evaluating oleaginous yeasts for enhanced microbial lipid production using sweetwater as a sustainable feedstock 2024 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s) 2024 Background Yeasts exhibit promising potential for the microbial conversion of crude glycerol, owing to their versatility in delivering a wide range of value-added products, particularly lipids. Sweetwater, a methanol-free by-product of the fat splitting process, has emerged as a promising alternative feedstock for the microbial utilization of crude glycerol. To further optimize sweetwater utilization, we compared the growth and lipid production capabilities of 21 oleaginous yeast strains under different conditions with various glycerol concentrations, sweetwater types and pH. Results We found that nutrient limitation and the unique carbon composition of sweetwater boosted significant lipid accumulation in several strains, in particular Rhodosporidium toruloides NRRL Y-6987. Subsequently, to decipher the underlying mechanism, the transcriptomic changes of R. toruloides NRRL Y-6987 were further analyzed, indicating potential sugars and oligopeptides in sweetwater supporting growth and lipid accumulation as well as exogenous fatty acid uptake leading to the enhanced lipid accumulation. Conclusion Our comparative study successfully demonstrated sweetwater as a cost-effective feedstock while identifying R. toluroides NRRL Y-6987 as a highly promising microbial oil producer. Furthermore, we also suggested potential sweetwater type and strain engineering targets that could potentially enhance microbial lipid production. Graphical abstract Oleaginous yeasts (dpeaa)DE-He213 Microbial oil (dpeaa)DE-He213 Microbial cell factories (dpeaa)DE-He213 Sweetwater (dpeaa)DE-He213 Comparative omics (dpeaa)DE-He213 Lee, Yi Qing (orcid)0000-0002-8970-0729 aut Lakshmanan, Meiyappan (orcid)0000-0003-2356-3458 aut Ow, Dave Siak-Wei (orcid)0000-0002-0436-1896 aut Staniland, Paul aut Staniland, Jessica aut Savill, Ian aut Tee, Kang Lan (orcid)0000-0001-9458-733X aut Wong, Tuck Seng (orcid)0000-0001-7689-9057 aut Lee, Dong-Yup (orcid)0000-0003-0901-708X aut Enthalten in Microbial cell factories London : Biomed Central, 2002 23(2024), 1 vom: 24. Feb. (DE-627)355987651 (DE-600)2091377-1 1475-2859 nnns volume:23 year:2024 number:1 day:24 month:02 https://dx.doi.org/10.1186/s12934-024-02336-x kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 23 2024 1 24 02
allfieldsSound	10.1186/s12934-024-02336-x doi (DE-627)SPR054887860 (SPR)s12934-024-02336-x-e DE-627 ger DE-627 rakwb eng Keita, Valériane Malika verfasserin aut Evaluating oleaginous yeasts for enhanced microbial lipid production using sweetwater as a sustainable feedstock 2024 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s) 2024 Background Yeasts exhibit promising potential for the microbial conversion of crude glycerol, owing to their versatility in delivering a wide range of value-added products, particularly lipids. Sweetwater, a methanol-free by-product of the fat splitting process, has emerged as a promising alternative feedstock for the microbial utilization of crude glycerol. To further optimize sweetwater utilization, we compared the growth and lipid production capabilities of 21 oleaginous yeast strains under different conditions with various glycerol concentrations, sweetwater types and pH. Results We found that nutrient limitation and the unique carbon composition of sweetwater boosted significant lipid accumulation in several strains, in particular Rhodosporidium toruloides NRRL Y-6987. Subsequently, to decipher the underlying mechanism, the transcriptomic changes of R. toruloides NRRL Y-6987 were further analyzed, indicating potential sugars and oligopeptides in sweetwater supporting growth and lipid accumulation as well as exogenous fatty acid uptake leading to the enhanced lipid accumulation. Conclusion Our comparative study successfully demonstrated sweetwater as a cost-effective feedstock while identifying R. toluroides NRRL Y-6987 as a highly promising microbial oil producer. Furthermore, we also suggested potential sweetwater type and strain engineering targets that could potentially enhance microbial lipid production. Graphical abstract Oleaginous yeasts (dpeaa)DE-He213 Microbial oil (dpeaa)DE-He213 Microbial cell factories (dpeaa)DE-He213 Sweetwater (dpeaa)DE-He213 Comparative omics (dpeaa)DE-He213 Lee, Yi Qing (orcid)0000-0002-8970-0729 aut Lakshmanan, Meiyappan (orcid)0000-0003-2356-3458 aut Ow, Dave Siak-Wei (orcid)0000-0002-0436-1896 aut Staniland, Paul aut Staniland, Jessica aut Savill, Ian aut Tee, Kang Lan (orcid)0000-0001-9458-733X aut Wong, Tuck Seng (orcid)0000-0001-7689-9057 aut Lee, Dong-Yup (orcid)0000-0003-0901-708X aut Enthalten in Microbial cell factories London : Biomed Central, 2002 23(2024), 1 vom: 24. Feb. (DE-627)355987651 (DE-600)2091377-1 1475-2859 nnns volume:23 year:2024 number:1 day:24 month:02 https://dx.doi.org/10.1186/s12934-024-02336-x kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 23 2024 1 24 02
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Sweetwater, a methanol-free by-product of the fat splitting process, has emerged as a promising alternative feedstock for the microbial utilization of crude glycerol. To further optimize sweetwater utilization, we compared the growth and lipid production capabilities of 21 oleaginous yeast strains under different conditions with various glycerol concentrations, sweetwater types and pH. Results We found that nutrient limitation and the unique carbon composition of sweetwater boosted significant lipid accumulation in several strains, in particular Rhodosporidium toruloides NRRL Y-6987. Subsequently, to decipher the underlying mechanism, the transcriptomic changes of R. toruloides NRRL Y-6987 were further analyzed, indicating potential sugars and oligopeptides in sweetwater supporting growth and lipid accumulation as well as exogenous fatty acid uptake leading to the enhanced lipid accumulation. Conclusion Our comparative study successfully demonstrated sweetwater as a cost-effective feedstock while identifying R. toluroides NRRL Y-6987 as a highly promising microbial oil producer. Furthermore, we also suggested potential sweetwater type and strain engineering targets that could potentially enhance microbial lipid production. 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author	Keita, Valériane Malika
spellingShingle	Keita, Valériane Malika misc Oleaginous yeasts misc Microbial oil misc Microbial cell factories misc Sweetwater misc Comparative omics Evaluating oleaginous yeasts for enhanced microbial lipid production using sweetwater as a sustainable feedstock
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topic_title	Evaluating oleaginous yeasts for enhanced microbial lipid production using sweetwater as a sustainable feedstock Oleaginous yeasts (dpeaa)DE-He213 Microbial oil (dpeaa)DE-He213 Microbial cell factories (dpeaa)DE-He213 Sweetwater (dpeaa)DE-He213 Comparative omics (dpeaa)DE-He213
topic	misc Oleaginous yeasts misc Microbial oil misc Microbial cell factories misc Sweetwater misc Comparative omics
topic_unstemmed	misc Oleaginous yeasts misc Microbial oil misc Microbial cell factories misc Sweetwater misc Comparative omics
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title	Evaluating oleaginous yeasts for enhanced microbial lipid production using sweetwater as a sustainable feedstock
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title_full	Evaluating oleaginous yeasts for enhanced microbial lipid production using sweetwater as a sustainable feedstock
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author_browse	Keita, Valériane Malika Lee, Yi Qing Lakshmanan, Meiyappan Ow, Dave Siak-Wei Staniland, Paul Staniland, Jessica Savill, Ian Tee, Kang Lan Wong, Tuck Seng Lee, Dong-Yup
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title_sort	evaluating oleaginous yeasts for enhanced microbial lipid production using sweetwater as a sustainable feedstock
title_auth	Evaluating oleaginous yeasts for enhanced microbial lipid production using sweetwater as a sustainable feedstock
abstract	Background Yeasts exhibit promising potential for the microbial conversion of crude glycerol, owing to their versatility in delivering a wide range of value-added products, particularly lipids. Sweetwater, a methanol-free by-product of the fat splitting process, has emerged as a promising alternative feedstock for the microbial utilization of crude glycerol. To further optimize sweetwater utilization, we compared the growth and lipid production capabilities of 21 oleaginous yeast strains under different conditions with various glycerol concentrations, sweetwater types and pH. Results We found that nutrient limitation and the unique carbon composition of sweetwater boosted significant lipid accumulation in several strains, in particular Rhodosporidium toruloides NRRL Y-6987. Subsequently, to decipher the underlying mechanism, the transcriptomic changes of R. toruloides NRRL Y-6987 were further analyzed, indicating potential sugars and oligopeptides in sweetwater supporting growth and lipid accumulation as well as exogenous fatty acid uptake leading to the enhanced lipid accumulation. Conclusion Our comparative study successfully demonstrated sweetwater as a cost-effective feedstock while identifying R. toluroides NRRL Y-6987 as a highly promising microbial oil producer. Furthermore, we also suggested potential sweetwater type and strain engineering targets that could potentially enhance microbial lipid production. Graphical abstract © The Author(s) 2024
abstractGer	Background Yeasts exhibit promising potential for the microbial conversion of crude glycerol, owing to their versatility in delivering a wide range of value-added products, particularly lipids. Sweetwater, a methanol-free by-product of the fat splitting process, has emerged as a promising alternative feedstock for the microbial utilization of crude glycerol. To further optimize sweetwater utilization, we compared the growth and lipid production capabilities of 21 oleaginous yeast strains under different conditions with various glycerol concentrations, sweetwater types and pH. Results We found that nutrient limitation and the unique carbon composition of sweetwater boosted significant lipid accumulation in several strains, in particular Rhodosporidium toruloides NRRL Y-6987. Subsequently, to decipher the underlying mechanism, the transcriptomic changes of R. toruloides NRRL Y-6987 were further analyzed, indicating potential sugars and oligopeptides in sweetwater supporting growth and lipid accumulation as well as exogenous fatty acid uptake leading to the enhanced lipid accumulation. Conclusion Our comparative study successfully demonstrated sweetwater as a cost-effective feedstock while identifying R. toluroides NRRL Y-6987 as a highly promising microbial oil producer. Furthermore, we also suggested potential sweetwater type and strain engineering targets that could potentially enhance microbial lipid production. Graphical abstract © The Author(s) 2024
abstract_unstemmed	Background Yeasts exhibit promising potential for the microbial conversion of crude glycerol, owing to their versatility in delivering a wide range of value-added products, particularly lipids. Sweetwater, a methanol-free by-product of the fat splitting process, has emerged as a promising alternative feedstock for the microbial utilization of crude glycerol. To further optimize sweetwater utilization, we compared the growth and lipid production capabilities of 21 oleaginous yeast strains under different conditions with various glycerol concentrations, sweetwater types and pH. Results We found that nutrient limitation and the unique carbon composition of sweetwater boosted significant lipid accumulation in several strains, in particular Rhodosporidium toruloides NRRL Y-6987. Subsequently, to decipher the underlying mechanism, the transcriptomic changes of R. toruloides NRRL Y-6987 were further analyzed, indicating potential sugars and oligopeptides in sweetwater supporting growth and lipid accumulation as well as exogenous fatty acid uptake leading to the enhanced lipid accumulation. Conclusion Our comparative study successfully demonstrated sweetwater as a cost-effective feedstock while identifying R. toluroides NRRL Y-6987 as a highly promising microbial oil producer. Furthermore, we also suggested potential sweetwater type and strain engineering targets that could potentially enhance microbial lipid production. Graphical abstract © The Author(s) 2024
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title_short	Evaluating oleaginous yeasts for enhanced microbial lipid production using sweetwater as a sustainable feedstock
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Sweetwater, a methanol-free by-product of the fat splitting process, has emerged as a promising alternative feedstock for the microbial utilization of crude glycerol. To further optimize sweetwater utilization, we compared the growth and lipid production capabilities of 21 oleaginous yeast strains under different conditions with various glycerol concentrations, sweetwater types and pH. Results We found that nutrient limitation and the unique carbon composition of sweetwater boosted significant lipid accumulation in several strains, in particular Rhodosporidium toruloides NRRL Y-6987. Subsequently, to decipher the underlying mechanism, the transcriptomic changes of R. toruloides NRRL Y-6987 were further analyzed, indicating potential sugars and oligopeptides in sweetwater supporting growth and lipid accumulation as well as exogenous fatty acid uptake leading to the enhanced lipid accumulation. Conclusion Our comparative study successfully demonstrated sweetwater as a cost-effective feedstock while identifying R. toluroides NRRL Y-6987 as a highly promising microbial oil producer. Furthermore, we also suggested potential sweetwater type and strain engineering targets that could potentially enhance microbial lipid production. 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Evaluating oleaginous yeasts for enhanced microbial lipid production using sweetwater as a sustainable feedstock

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