Acremonium sp. diglycosidase-aid chemical diversification: valorization of industry by-products

Abstract The fungal diglycosidase α-rhamnosyl-β-glucosidase I (αRβG I) from Acremonium sp. DSM 24697 catalyzes the glycosylation of various OH-acceptors using the citrus flavanone hesperidin. We successfully applied a one-pot biocatalysis process to synthesize 4-methylumbellipheryl rutinoside (4-MUR...
Ausführliche Beschreibung

Gespeichert in:

Autor*in:	Baglioni, Micaela [verfasserIn] Fries, Alexander Müller, Jan-Mathis Omarini, Alejandra Müller, Michael Breccia, Javier D. Mazzaferro, Laura S.

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2024

Schlagwörter:	Polyalcohols Transglycosylation Substrate specificity Glycosylation/hydrolysis selectivity

Anmerkung:	© The Author(s) 2024

Übergeordnetes Werk:	Enthalten in: Applied microbiology and biotechnology - Berlin : Springer, 1975, 108(2024), 1 vom: 02. März
Übergeordnetes Werk:	volume:108 ; year:2024 ; number:1 ; day:02 ; month:03

Links:	Volltext

DOI / URN:	10.1007/s00253-023-12957-8

Katalog-ID:	SPR054989736

Internformat


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520			\|a Abstract The fungal diglycosidase α-rhamnosyl-β-glucosidase I (αRβG I) from Acremonium sp. DSM 24697 catalyzes the glycosylation of various OH-acceptors using the citrus flavanone hesperidin. We successfully applied a one-pot biocatalysis process to synthesize 4-methylumbellipheryl rutinoside (4-MUR) and glyceryl rutinoside using a citrus peel residue as sugar donor. This residue, which contained 3.5 % [w/w] hesperidin, is the remaining of citrus processing after producing orange juice, essential oil, and peel-juice. The low-cost compound glycerol was utilized in the synthesis of glyceryl rutinoside. We implemented a simple method for the obtention of glyceryl rutinoside with 99 % yield, and its purification involving activated charcoal, which also facilitated the recovery of the by-product hesperetin through liquid-liquid extraction. This process presents a promising alternative for biorefinery operations, highlighting the valuable role of αRβG I in valorizing glycerol and agricultural by-products. Keypoints • αRβG I catalyzed the synthesis of rutinosides using a suspension of OPW as sugar donor. • The glycosylation of aliphatic polyalcohols by the αRβG I resulted in products bearing a single rutinose moiety. • αRβG I catalyzed the synthesis of glyceryl rutinoside with high glycosylation/hydrolysis selectivity (99 % yield). Graphical Abstract
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700	1		\|a Omarini, Alejandra \|4 aut
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700	1		\|a Mazzaferro, Laura S. \|0 (orcid)0000-0002-9323-8549 \|4 aut
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912			\|a GBV_ILN_267
912			\|a GBV_ILN_285
912			\|a GBV_ILN_293
912			\|a GBV_ILN_370
912			\|a GBV_ILN_381
912			\|a GBV_ILN_602
912			\|a GBV_ILN_702
912			\|a GBV_ILN_2001
912			\|a GBV_ILN_2003
912			\|a GBV_ILN_2005
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912			\|a GBV_ILN_4246
912			\|a GBV_ILN_4249
912			\|a GBV_ILN_4305
912			\|a GBV_ILN_4306
912			\|a GBV_ILN_4307
912			\|a GBV_ILN_4313
912			\|a GBV_ILN_4322
912			\|a GBV_ILN_4323
912			\|a GBV_ILN_4324
912			\|a GBV_ILN_4338
912			\|a GBV_ILN_4367
912			\|a GBV_ILN_4700
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allfields	10.1007/s00253-023-12957-8 doi (DE-627)SPR054989736 (SPR)s00253-023-12957-8-e DE-627 ger DE-627 rakwb eng Baglioni, Micaela verfasserin aut Acremonium sp. diglycosidase-aid chemical diversification: valorization of industry by-products 2024 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s) 2024 Abstract The fungal diglycosidase α-rhamnosyl-β-glucosidase I (αRβG I) from Acremonium sp. DSM 24697 catalyzes the glycosylation of various OH-acceptors using the citrus flavanone hesperidin. We successfully applied a one-pot biocatalysis process to synthesize 4-methylumbellipheryl rutinoside (4-MUR) and glyceryl rutinoside using a citrus peel residue as sugar donor. This residue, which contained 3.5 % [w/w] hesperidin, is the remaining of citrus processing after producing orange juice, essential oil, and peel-juice. The low-cost compound glycerol was utilized in the synthesis of glyceryl rutinoside. We implemented a simple method for the obtention of glyceryl rutinoside with 99 % yield, and its purification involving activated charcoal, which also facilitated the recovery of the by-product hesperetin through liquid-liquid extraction. This process presents a promising alternative for biorefinery operations, highlighting the valuable role of αRβG I in valorizing glycerol and agricultural by-products. Keypoints • αRβG I catalyzed the synthesis of rutinosides using a suspension of OPW as sugar donor. • The glycosylation of aliphatic polyalcohols by the αRβG I resulted in products bearing a single rutinose moiety. • αRβG I catalyzed the synthesis of glyceryl rutinoside with high glycosylation/hydrolysis selectivity (99 % yield). Graphical Abstract Polyalcohols (dpeaa)DE-He213 Transglycosylation (dpeaa)DE-He213 Substrate specificity (dpeaa)DE-He213 Glycosylation/hydrolysis selectivity (dpeaa)DE-He213 Fries, Alexander aut Müller, Jan-Mathis aut Omarini, Alejandra aut Müller, Michael aut Breccia, Javier D. aut Mazzaferro, Laura S. (orcid)0000-0002-9323-8549 aut Enthalten in Applied microbiology and biotechnology Berlin : Springer, 1975 108(2024), 1 vom: 02. März (DE-627)265509564 (DE-600)1464336-4 1432-0614 nnns volume:108 year:2024 number:1 day:02 month:03 https://dx.doi.org/10.1007/s00253-023-12957-8 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_165 GBV_ILN_170 GBV_ILN_187 GBV_ILN_213 GBV_ILN_230 GBV_ILN_267 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_381 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2119 GBV_ILN_2129 GBV_ILN_2147 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2336 GBV_ILN_2360 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 108 2024 1 02 03
spelling	10.1007/s00253-023-12957-8 doi (DE-627)SPR054989736 (SPR)s00253-023-12957-8-e DE-627 ger DE-627 rakwb eng Baglioni, Micaela verfasserin aut Acremonium sp. diglycosidase-aid chemical diversification: valorization of industry by-products 2024 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s) 2024 Abstract The fungal diglycosidase α-rhamnosyl-β-glucosidase I (αRβG I) from Acremonium sp. DSM 24697 catalyzes the glycosylation of various OH-acceptors using the citrus flavanone hesperidin. We successfully applied a one-pot biocatalysis process to synthesize 4-methylumbellipheryl rutinoside (4-MUR) and glyceryl rutinoside using a citrus peel residue as sugar donor. This residue, which contained 3.5 % [w/w] hesperidin, is the remaining of citrus processing after producing orange juice, essential oil, and peel-juice. The low-cost compound glycerol was utilized in the synthesis of glyceryl rutinoside. We implemented a simple method for the obtention of glyceryl rutinoside with 99 % yield, and its purification involving activated charcoal, which also facilitated the recovery of the by-product hesperetin through liquid-liquid extraction. This process presents a promising alternative for biorefinery operations, highlighting the valuable role of αRβG I in valorizing glycerol and agricultural by-products. Keypoints • αRβG I catalyzed the synthesis of rutinosides using a suspension of OPW as sugar donor. • The glycosylation of aliphatic polyalcohols by the αRβG I resulted in products bearing a single rutinose moiety. • αRβG I catalyzed the synthesis of glyceryl rutinoside with high glycosylation/hydrolysis selectivity (99 % yield). Graphical Abstract Polyalcohols (dpeaa)DE-He213 Transglycosylation (dpeaa)DE-He213 Substrate specificity (dpeaa)DE-He213 Glycosylation/hydrolysis selectivity (dpeaa)DE-He213 Fries, Alexander aut Müller, Jan-Mathis aut Omarini, Alejandra aut Müller, Michael aut Breccia, Javier D. aut Mazzaferro, Laura S. (orcid)0000-0002-9323-8549 aut Enthalten in Applied microbiology and biotechnology Berlin : Springer, 1975 108(2024), 1 vom: 02. März (DE-627)265509564 (DE-600)1464336-4 1432-0614 nnns volume:108 year:2024 number:1 day:02 month:03 https://dx.doi.org/10.1007/s00253-023-12957-8 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_165 GBV_ILN_170 GBV_ILN_187 GBV_ILN_213 GBV_ILN_230 GBV_ILN_267 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_381 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2119 GBV_ILN_2129 GBV_ILN_2147 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2336 GBV_ILN_2360 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 108 2024 1 02 03
allfields_unstemmed	10.1007/s00253-023-12957-8 doi (DE-627)SPR054989736 (SPR)s00253-023-12957-8-e DE-627 ger DE-627 rakwb eng Baglioni, Micaela verfasserin aut Acremonium sp. diglycosidase-aid chemical diversification: valorization of industry by-products 2024 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s) 2024 Abstract The fungal diglycosidase α-rhamnosyl-β-glucosidase I (αRβG I) from Acremonium sp. DSM 24697 catalyzes the glycosylation of various OH-acceptors using the citrus flavanone hesperidin. We successfully applied a one-pot biocatalysis process to synthesize 4-methylumbellipheryl rutinoside (4-MUR) and glyceryl rutinoside using a citrus peel residue as sugar donor. This residue, which contained 3.5 % [w/w] hesperidin, is the remaining of citrus processing after producing orange juice, essential oil, and peel-juice. The low-cost compound glycerol was utilized in the synthesis of glyceryl rutinoside. We implemented a simple method for the obtention of glyceryl rutinoside with 99 % yield, and its purification involving activated charcoal, which also facilitated the recovery of the by-product hesperetin through liquid-liquid extraction. This process presents a promising alternative for biorefinery operations, highlighting the valuable role of αRβG I in valorizing glycerol and agricultural by-products. Keypoints • αRβG I catalyzed the synthesis of rutinosides using a suspension of OPW as sugar donor. • The glycosylation of aliphatic polyalcohols by the αRβG I resulted in products bearing a single rutinose moiety. • αRβG I catalyzed the synthesis of glyceryl rutinoside with high glycosylation/hydrolysis selectivity (99 % yield). Graphical Abstract Polyalcohols (dpeaa)DE-He213 Transglycosylation (dpeaa)DE-He213 Substrate specificity (dpeaa)DE-He213 Glycosylation/hydrolysis selectivity (dpeaa)DE-He213 Fries, Alexander aut Müller, Jan-Mathis aut Omarini, Alejandra aut Müller, Michael aut Breccia, Javier D. aut Mazzaferro, Laura S. (orcid)0000-0002-9323-8549 aut Enthalten in Applied microbiology and biotechnology Berlin : Springer, 1975 108(2024), 1 vom: 02. März (DE-627)265509564 (DE-600)1464336-4 1432-0614 nnns volume:108 year:2024 number:1 day:02 month:03 https://dx.doi.org/10.1007/s00253-023-12957-8 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_165 GBV_ILN_170 GBV_ILN_187 GBV_ILN_213 GBV_ILN_230 GBV_ILN_267 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_381 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2119 GBV_ILN_2129 GBV_ILN_2147 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2336 GBV_ILN_2360 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 108 2024 1 02 03
allfieldsGer	10.1007/s00253-023-12957-8 doi (DE-627)SPR054989736 (SPR)s00253-023-12957-8-e DE-627 ger DE-627 rakwb eng Baglioni, Micaela verfasserin aut Acremonium sp. diglycosidase-aid chemical diversification: valorization of industry by-products 2024 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s) 2024 Abstract The fungal diglycosidase α-rhamnosyl-β-glucosidase I (αRβG I) from Acremonium sp. DSM 24697 catalyzes the glycosylation of various OH-acceptors using the citrus flavanone hesperidin. We successfully applied a one-pot biocatalysis process to synthesize 4-methylumbellipheryl rutinoside (4-MUR) and glyceryl rutinoside using a citrus peel residue as sugar donor. This residue, which contained 3.5 % [w/w] hesperidin, is the remaining of citrus processing after producing orange juice, essential oil, and peel-juice. The low-cost compound glycerol was utilized in the synthesis of glyceryl rutinoside. We implemented a simple method for the obtention of glyceryl rutinoside with 99 % yield, and its purification involving activated charcoal, which also facilitated the recovery of the by-product hesperetin through liquid-liquid extraction. This process presents a promising alternative for biorefinery operations, highlighting the valuable role of αRβG I in valorizing glycerol and agricultural by-products. Keypoints • αRβG I catalyzed the synthesis of rutinosides using a suspension of OPW as sugar donor. • The glycosylation of aliphatic polyalcohols by the αRβG I resulted in products bearing a single rutinose moiety. • αRβG I catalyzed the synthesis of glyceryl rutinoside with high glycosylation/hydrolysis selectivity (99 % yield). Graphical Abstract Polyalcohols (dpeaa)DE-He213 Transglycosylation (dpeaa)DE-He213 Substrate specificity (dpeaa)DE-He213 Glycosylation/hydrolysis selectivity (dpeaa)DE-He213 Fries, Alexander aut Müller, Jan-Mathis aut Omarini, Alejandra aut Müller, Michael aut Breccia, Javier D. aut Mazzaferro, Laura S. (orcid)0000-0002-9323-8549 aut Enthalten in Applied microbiology and biotechnology Berlin : Springer, 1975 108(2024), 1 vom: 02. März (DE-627)265509564 (DE-600)1464336-4 1432-0614 nnns volume:108 year:2024 number:1 day:02 month:03 https://dx.doi.org/10.1007/s00253-023-12957-8 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_165 GBV_ILN_170 GBV_ILN_187 GBV_ILN_213 GBV_ILN_230 GBV_ILN_267 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_381 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2119 GBV_ILN_2129 GBV_ILN_2147 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2336 GBV_ILN_2360 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 108 2024 1 02 03
allfieldsSound	10.1007/s00253-023-12957-8 doi (DE-627)SPR054989736 (SPR)s00253-023-12957-8-e DE-627 ger DE-627 rakwb eng Baglioni, Micaela verfasserin aut Acremonium sp. diglycosidase-aid chemical diversification: valorization of industry by-products 2024 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s) 2024 Abstract The fungal diglycosidase α-rhamnosyl-β-glucosidase I (αRβG I) from Acremonium sp. DSM 24697 catalyzes the glycosylation of various OH-acceptors using the citrus flavanone hesperidin. We successfully applied a one-pot biocatalysis process to synthesize 4-methylumbellipheryl rutinoside (4-MUR) and glyceryl rutinoside using a citrus peel residue as sugar donor. This residue, which contained 3.5 % [w/w] hesperidin, is the remaining of citrus processing after producing orange juice, essential oil, and peel-juice. The low-cost compound glycerol was utilized in the synthesis of glyceryl rutinoside. We implemented a simple method for the obtention of glyceryl rutinoside with 99 % yield, and its purification involving activated charcoal, which also facilitated the recovery of the by-product hesperetin through liquid-liquid extraction. This process presents a promising alternative for biorefinery operations, highlighting the valuable role of αRβG I in valorizing glycerol and agricultural by-products. Keypoints • αRβG I catalyzed the synthesis of rutinosides using a suspension of OPW as sugar donor. • The glycosylation of aliphatic polyalcohols by the αRβG I resulted in products bearing a single rutinose moiety. • αRβG I catalyzed the synthesis of glyceryl rutinoside with high glycosylation/hydrolysis selectivity (99 % yield). Graphical Abstract Polyalcohols (dpeaa)DE-He213 Transglycosylation (dpeaa)DE-He213 Substrate specificity (dpeaa)DE-He213 Glycosylation/hydrolysis selectivity (dpeaa)DE-He213 Fries, Alexander aut Müller, Jan-Mathis aut Omarini, Alejandra aut Müller, Michael aut Breccia, Javier D. aut Mazzaferro, Laura S. (orcid)0000-0002-9323-8549 aut Enthalten in Applied microbiology and biotechnology Berlin : Springer, 1975 108(2024), 1 vom: 02. März (DE-627)265509564 (DE-600)1464336-4 1432-0614 nnns volume:108 year:2024 number:1 day:02 month:03 https://dx.doi.org/10.1007/s00253-023-12957-8 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_165 GBV_ILN_170 GBV_ILN_187 GBV_ILN_213 GBV_ILN_230 GBV_ILN_267 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_381 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2119 GBV_ILN_2129 GBV_ILN_2147 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2336 GBV_ILN_2360 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 108 2024 1 02 03
language	English
source	Enthalten in Applied microbiology and biotechnology 108(2024), 1 vom: 02. März volume:108 year:2024 number:1 day:02 month:03
sourceStr	Enthalten in Applied microbiology and biotechnology 108(2024), 1 vom: 02. März volume:108 year:2024 number:1 day:02 month:03
format_phy_str_mv	Article
institution	findex.gbv.de
topic_facet	Polyalcohols Transglycosylation Substrate specificity Glycosylation/hydrolysis selectivity
isfreeaccess_bool	true
container_title	Applied microbiology and biotechnology
authorswithroles_txt_mv	Baglioni, Micaela @@aut@@ Fries, Alexander @@aut@@ Müller, Jan-Mathis @@aut@@ Omarini, Alejandra @@aut@@ Müller, Michael @@aut@@ Breccia, Javier D. @@aut@@ Mazzaferro, Laura S. @@aut@@
publishDateDaySort_date	2024-03-02T00:00:00Z
hierarchy_top_id	265509564
id	SPR054989736
language_de	englisch
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DSM 24697 catalyzes the glycosylation of various OH-acceptors using the citrus flavanone hesperidin. We successfully applied a one-pot biocatalysis process to synthesize 4-methylumbellipheryl rutinoside (4-MUR) and glyceryl rutinoside using a citrus peel residue as sugar donor. This residue, which contained 3.5 % [w/w] hesperidin, is the remaining of citrus processing after producing orange juice, essential oil, and peel-juice. The low-cost compound glycerol was utilized in the synthesis of glyceryl rutinoside. We implemented a simple method for the obtention of glyceryl rutinoside with 99 % yield, and its purification involving activated charcoal, which also facilitated the recovery of the by-product hesperetin through liquid-liquid extraction. This process presents a promising alternative for biorefinery operations, highlighting the valuable role of αRβG I in valorizing glycerol and agricultural by-products. Keypoints • αRβG I catalyzed the synthesis of rutinosides using a suspension of OPW as sugar donor. • The glycosylation of aliphatic polyalcohols by the αRβG I resulted in products bearing a single rutinose moiety. • αRβG I catalyzed the synthesis of glyceryl rutinoside with high glycosylation/hydrolysis selectivity (99 % yield). 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author	Baglioni, Micaela
spellingShingle	Baglioni, Micaela misc Polyalcohols misc Transglycosylation misc Substrate specificity misc Glycosylation/hydrolysis selectivity Acremonium sp. diglycosidase-aid chemical diversification: valorization of industry by-products
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topic_title	Acremonium sp. diglycosidase-aid chemical diversification: valorization of industry by-products Polyalcohols (dpeaa)DE-He213 Transglycosylation (dpeaa)DE-He213 Substrate specificity (dpeaa)DE-He213 Glycosylation/hydrolysis selectivity (dpeaa)DE-He213
topic	misc Polyalcohols misc Transglycosylation misc Substrate specificity misc Glycosylation/hydrolysis selectivity
topic_unstemmed	misc Polyalcohols misc Transglycosylation misc Substrate specificity misc Glycosylation/hydrolysis selectivity
topic_browse	misc Polyalcohols misc Transglycosylation misc Substrate specificity misc Glycosylation/hydrolysis selectivity
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title	Acremonium sp. diglycosidase-aid chemical diversification: valorization of industry by-products
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title_full	Acremonium sp. diglycosidase-aid chemical diversification: valorization of industry by-products
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author_browse	Baglioni, Micaela Fries, Alexander Müller, Jan-Mathis Omarini, Alejandra Müller, Michael Breccia, Javier D. Mazzaferro, Laura S.
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title_sort	acremonium sp. diglycosidase-aid chemical diversification: valorization of industry by-products
title_auth	Acremonium sp. diglycosidase-aid chemical diversification: valorization of industry by-products
abstract	Abstract The fungal diglycosidase α-rhamnosyl-β-glucosidase I (αRβG I) from Acremonium sp. DSM 24697 catalyzes the glycosylation of various OH-acceptors using the citrus flavanone hesperidin. We successfully applied a one-pot biocatalysis process to synthesize 4-methylumbellipheryl rutinoside (4-MUR) and glyceryl rutinoside using a citrus peel residue as sugar donor. This residue, which contained 3.5 % [w/w] hesperidin, is the remaining of citrus processing after producing orange juice, essential oil, and peel-juice. The low-cost compound glycerol was utilized in the synthesis of glyceryl rutinoside. We implemented a simple method for the obtention of glyceryl rutinoside with 99 % yield, and its purification involving activated charcoal, which also facilitated the recovery of the by-product hesperetin through liquid-liquid extraction. This process presents a promising alternative for biorefinery operations, highlighting the valuable role of αRβG I in valorizing glycerol and agricultural by-products. Keypoints • αRβG I catalyzed the synthesis of rutinosides using a suspension of OPW as sugar donor. • The glycosylation of aliphatic polyalcohols by the αRβG I resulted in products bearing a single rutinose moiety. • αRβG I catalyzed the synthesis of glyceryl rutinoside with high glycosylation/hydrolysis selectivity (99 % yield). Graphical Abstract © The Author(s) 2024
abstractGer	Abstract The fungal diglycosidase α-rhamnosyl-β-glucosidase I (αRβG I) from Acremonium sp. DSM 24697 catalyzes the glycosylation of various OH-acceptors using the citrus flavanone hesperidin. We successfully applied a one-pot biocatalysis process to synthesize 4-methylumbellipheryl rutinoside (4-MUR) and glyceryl rutinoside using a citrus peel residue as sugar donor. This residue, which contained 3.5 % [w/w] hesperidin, is the remaining of citrus processing after producing orange juice, essential oil, and peel-juice. The low-cost compound glycerol was utilized in the synthesis of glyceryl rutinoside. We implemented a simple method for the obtention of glyceryl rutinoside with 99 % yield, and its purification involving activated charcoal, which also facilitated the recovery of the by-product hesperetin through liquid-liquid extraction. This process presents a promising alternative for biorefinery operations, highlighting the valuable role of αRβG I in valorizing glycerol and agricultural by-products. Keypoints • αRβG I catalyzed the synthesis of rutinosides using a suspension of OPW as sugar donor. • The glycosylation of aliphatic polyalcohols by the αRβG I resulted in products bearing a single rutinose moiety. • αRβG I catalyzed the synthesis of glyceryl rutinoside with high glycosylation/hydrolysis selectivity (99 % yield). Graphical Abstract © The Author(s) 2024
abstract_unstemmed	Abstract The fungal diglycosidase α-rhamnosyl-β-glucosidase I (αRβG I) from Acremonium sp. DSM 24697 catalyzes the glycosylation of various OH-acceptors using the citrus flavanone hesperidin. We successfully applied a one-pot biocatalysis process to synthesize 4-methylumbellipheryl rutinoside (4-MUR) and glyceryl rutinoside using a citrus peel residue as sugar donor. This residue, which contained 3.5 % [w/w] hesperidin, is the remaining of citrus processing after producing orange juice, essential oil, and peel-juice. The low-cost compound glycerol was utilized in the synthesis of glyceryl rutinoside. We implemented a simple method for the obtention of glyceryl rutinoside with 99 % yield, and its purification involving activated charcoal, which also facilitated the recovery of the by-product hesperetin through liquid-liquid extraction. This process presents a promising alternative for biorefinery operations, highlighting the valuable role of αRβG I in valorizing glycerol and agricultural by-products. Keypoints • αRβG I catalyzed the synthesis of rutinosides using a suspension of OPW as sugar donor. • The glycosylation of aliphatic polyalcohols by the αRβG I resulted in products bearing a single rutinose moiety. • αRβG I catalyzed the synthesis of glyceryl rutinoside with high glycosylation/hydrolysis selectivity (99 % yield). Graphical Abstract © The Author(s) 2024
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title_short	Acremonium sp. diglycosidase-aid chemical diversification: valorization of industry by-products
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author2	Fries, Alexander Müller, Jan-Mathis Omarini, Alejandra Müller, Michael Breccia, Javier D. Mazzaferro, Laura S.
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