Cleaved caspase-3 is present in the majority of glial cells in the intact rat spinal cord during postnatal life
Abstract Cell death is an essential process that occurs during the development of the central nervous system. Despite the availability of a wide range of commercially produced antibodies against various apoptotic markers, data regarding apoptosis in intact spinal cord during postnatal development an...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
Holota, R. [verfasserIn] |
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| Format: |
E-Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
2023 |
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| Schlagwörter: |
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| Anmerkung: |
© The Author(s) 2023 |
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| Übergeordnetes Werk: |
Enthalten in: Histochemistry and cell biology - Berlin : Springer, 1958, 161(2023), 3 vom: 08. Nov., Seite 269-286 |
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| Übergeordnetes Werk: |
volume:161 ; year:2023 ; number:3 ; day:08 ; month:11 ; pages:269-286 |
| Links: |
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| DOI / URN: |
10.1007/s00418-023-02249-7 |
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| Katalog-ID: |
SPR055002897 |
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| 100 | 1 | |a Holota, R. |e verfasserin |0 (orcid)0009-0002-5942-4441 |4 aut | |
| 245 | 1 | 0 | |a Cleaved caspase-3 is present in the majority of glial cells in the intact rat spinal cord during postnatal life |
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| 520 | |a Abstract Cell death is an essential process that occurs during the development of the central nervous system. Despite the availability of a wide range of commercially produced antibodies against various apoptotic markers, data regarding apoptosis in intact spinal cord during postnatal development and adulthood are mostly missing. We investigated apoptosis in rat spinal cord at different stages of ontogenesis (postnatal days 8, 29, and 90). For this purpose, we applied immunofluorescent detection of two widely used apoptotic markers, cleaved caspase-3 (cC3) and cleaved poly(ADP-ribose) polymerase (cPARP). Surprisingly, we found significant discrepancy between the number of $ cC3^{+} $ cells and $ PARP^{+} $ cells, with a ratio between 500:1 and 5000:1 in rat spinal cord at all postnatal time points. The majority of $ cC3^{+} $ cells were glial cells and did not exhibit an apoptotic phenotype. In contrast with in vivo results, in vitro analysis of primary cell cultures derived from neonatal rat spinal cord and treated with the apoptotic inductor staurosporine revealed a similar onset of occurrence of both cC3 and cPARP in cells subjected to apoptosis. Gene expression analysis of spinal cord revealed elevated expression of the Birc4 (XIAP), Birc2, and Birc5 (Survivin) genes, which are known potent inhibitors of apoptosis. Our data indicate that cC3 is not an exclusive marker of apoptosis, especially in glial cells, owing its possible presence in inhibited forms and/or its participation in other non-apoptotic roles. Therefore, cPARP appears to be a more appropriate marker to detect apoptosis. | ||
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| 650 | 4 | |a Cleaved caspase-3 |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a Cleaved PARP |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a Spinal cord |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a Rat |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a Development |7 (dpeaa)DE-He213 | |
| 700 | 1 | |a Dečmanová, V. |0 (orcid)0000-0003-0281-5126 |4 aut | |
| 700 | 1 | |a Alexovič Matiašová, A. |0 (orcid)0000-0002-5500-4549 |4 aut | |
| 700 | 1 | |a Košuth, J. |0 (orcid)0000-0001-5584-8728 |4 aut | |
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| 700 | 1 | |a Daxnerová, Z. |0 (orcid)0000-0002-5778-8033 |4 aut | |
| 700 | 1 | |a Ševc, J. |0 (orcid)0000-0001-8050-4185 |4 aut | |
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10.1007/s00418-023-02249-7 doi (DE-627)SPR055002897 (SPR)s00418-023-02249-7-e DE-627 ger DE-627 rakwb eng Holota, R. verfasserin (orcid)0009-0002-5942-4441 aut Cleaved caspase-3 is present in the majority of glial cells in the intact rat spinal cord during postnatal life 2023 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s) 2023 Abstract Cell death is an essential process that occurs during the development of the central nervous system. Despite the availability of a wide range of commercially produced antibodies against various apoptotic markers, data regarding apoptosis in intact spinal cord during postnatal development and adulthood are mostly missing. We investigated apoptosis in rat spinal cord at different stages of ontogenesis (postnatal days 8, 29, and 90). For this purpose, we applied immunofluorescent detection of two widely used apoptotic markers, cleaved caspase-3 (cC3) and cleaved poly(ADP-ribose) polymerase (cPARP). Surprisingly, we found significant discrepancy between the number of $ cC3^{+} $ cells and $ PARP^{+} $ cells, with a ratio between 500:1 and 5000:1 in rat spinal cord at all postnatal time points. The majority of $ cC3^{+} $ cells were glial cells and did not exhibit an apoptotic phenotype. In contrast with in vivo results, in vitro analysis of primary cell cultures derived from neonatal rat spinal cord and treated with the apoptotic inductor staurosporine revealed a similar onset of occurrence of both cC3 and cPARP in cells subjected to apoptosis. Gene expression analysis of spinal cord revealed elevated expression of the Birc4 (XIAP), Birc2, and Birc5 (Survivin) genes, which are known potent inhibitors of apoptosis. Our data indicate that cC3 is not an exclusive marker of apoptosis, especially in glial cells, owing its possible presence in inhibited forms and/or its participation in other non-apoptotic roles. Therefore, cPARP appears to be a more appropriate marker to detect apoptosis. Apoptosis (dpeaa)DE-He213 Cleaved caspase-3 (dpeaa)DE-He213 Cleaved PARP (dpeaa)DE-He213 Spinal cord (dpeaa)DE-He213 Rat (dpeaa)DE-He213 Development (dpeaa)DE-He213 Dečmanová, V. (orcid)0000-0003-0281-5126 aut Alexovič Matiašová, A. (orcid)0000-0002-5500-4549 aut Košuth, J. (orcid)0000-0001-5584-8728 aut Slovinská, L. (orcid)0000-0002-9945-0655 aut Pačut, L. aut Tomori, Z. (orcid)0000-0002-0685-0247 aut Daxnerová, Z. (orcid)0000-0002-5778-8033 aut Ševc, J. (orcid)0000-0001-8050-4185 aut Enthalten in Histochemistry and cell biology Berlin : Springer, 1958 161(2023), 3 vom: 08. Nov., Seite 269-286 (DE-627)235503568 (DE-600)1398345-3 1432-119X nnns volume:161 year:2023 number:3 day:08 month:11 pages:269-286 https://dx.doi.org/10.1007/s00418-023-02249-7 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_267 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 161 2023 3 08 11 269-286 |
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10.1007/s00418-023-02249-7 doi (DE-627)SPR055002897 (SPR)s00418-023-02249-7-e DE-627 ger DE-627 rakwb eng Holota, R. verfasserin (orcid)0009-0002-5942-4441 aut Cleaved caspase-3 is present in the majority of glial cells in the intact rat spinal cord during postnatal life 2023 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s) 2023 Abstract Cell death is an essential process that occurs during the development of the central nervous system. Despite the availability of a wide range of commercially produced antibodies against various apoptotic markers, data regarding apoptosis in intact spinal cord during postnatal development and adulthood are mostly missing. We investigated apoptosis in rat spinal cord at different stages of ontogenesis (postnatal days 8, 29, and 90). For this purpose, we applied immunofluorescent detection of two widely used apoptotic markers, cleaved caspase-3 (cC3) and cleaved poly(ADP-ribose) polymerase (cPARP). Surprisingly, we found significant discrepancy between the number of $ cC3^{+} $ cells and $ PARP^{+} $ cells, with a ratio between 500:1 and 5000:1 in rat spinal cord at all postnatal time points. The majority of $ cC3^{+} $ cells were glial cells and did not exhibit an apoptotic phenotype. In contrast with in vivo results, in vitro analysis of primary cell cultures derived from neonatal rat spinal cord and treated with the apoptotic inductor staurosporine revealed a similar onset of occurrence of both cC3 and cPARP in cells subjected to apoptosis. Gene expression analysis of spinal cord revealed elevated expression of the Birc4 (XIAP), Birc2, and Birc5 (Survivin) genes, which are known potent inhibitors of apoptosis. Our data indicate that cC3 is not an exclusive marker of apoptosis, especially in glial cells, owing its possible presence in inhibited forms and/or its participation in other non-apoptotic roles. Therefore, cPARP appears to be a more appropriate marker to detect apoptosis. Apoptosis (dpeaa)DE-He213 Cleaved caspase-3 (dpeaa)DE-He213 Cleaved PARP (dpeaa)DE-He213 Spinal cord (dpeaa)DE-He213 Rat (dpeaa)DE-He213 Development (dpeaa)DE-He213 Dečmanová, V. (orcid)0000-0003-0281-5126 aut Alexovič Matiašová, A. (orcid)0000-0002-5500-4549 aut Košuth, J. (orcid)0000-0001-5584-8728 aut Slovinská, L. (orcid)0000-0002-9945-0655 aut Pačut, L. aut Tomori, Z. (orcid)0000-0002-0685-0247 aut Daxnerová, Z. (orcid)0000-0002-5778-8033 aut Ševc, J. (orcid)0000-0001-8050-4185 aut Enthalten in Histochemistry and cell biology Berlin : Springer, 1958 161(2023), 3 vom: 08. Nov., Seite 269-286 (DE-627)235503568 (DE-600)1398345-3 1432-119X nnns volume:161 year:2023 number:3 day:08 month:11 pages:269-286 https://dx.doi.org/10.1007/s00418-023-02249-7 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_267 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 161 2023 3 08 11 269-286 |
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10.1007/s00418-023-02249-7 doi (DE-627)SPR055002897 (SPR)s00418-023-02249-7-e DE-627 ger DE-627 rakwb eng Holota, R. verfasserin (orcid)0009-0002-5942-4441 aut Cleaved caspase-3 is present in the majority of glial cells in the intact rat spinal cord during postnatal life 2023 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s) 2023 Abstract Cell death is an essential process that occurs during the development of the central nervous system. Despite the availability of a wide range of commercially produced antibodies against various apoptotic markers, data regarding apoptosis in intact spinal cord during postnatal development and adulthood are mostly missing. We investigated apoptosis in rat spinal cord at different stages of ontogenesis (postnatal days 8, 29, and 90). For this purpose, we applied immunofluorescent detection of two widely used apoptotic markers, cleaved caspase-3 (cC3) and cleaved poly(ADP-ribose) polymerase (cPARP). Surprisingly, we found significant discrepancy between the number of $ cC3^{+} $ cells and $ PARP^{+} $ cells, with a ratio between 500:1 and 5000:1 in rat spinal cord at all postnatal time points. The majority of $ cC3^{+} $ cells were glial cells and did not exhibit an apoptotic phenotype. In contrast with in vivo results, in vitro analysis of primary cell cultures derived from neonatal rat spinal cord and treated with the apoptotic inductor staurosporine revealed a similar onset of occurrence of both cC3 and cPARP in cells subjected to apoptosis. Gene expression analysis of spinal cord revealed elevated expression of the Birc4 (XIAP), Birc2, and Birc5 (Survivin) genes, which are known potent inhibitors of apoptosis. Our data indicate that cC3 is not an exclusive marker of apoptosis, especially in glial cells, owing its possible presence in inhibited forms and/or its participation in other non-apoptotic roles. Therefore, cPARP appears to be a more appropriate marker to detect apoptosis. Apoptosis (dpeaa)DE-He213 Cleaved caspase-3 (dpeaa)DE-He213 Cleaved PARP (dpeaa)DE-He213 Spinal cord (dpeaa)DE-He213 Rat (dpeaa)DE-He213 Development (dpeaa)DE-He213 Dečmanová, V. (orcid)0000-0003-0281-5126 aut Alexovič Matiašová, A. (orcid)0000-0002-5500-4549 aut Košuth, J. (orcid)0000-0001-5584-8728 aut Slovinská, L. (orcid)0000-0002-9945-0655 aut Pačut, L. aut Tomori, Z. (orcid)0000-0002-0685-0247 aut Daxnerová, Z. (orcid)0000-0002-5778-8033 aut Ševc, J. (orcid)0000-0001-8050-4185 aut Enthalten in Histochemistry and cell biology Berlin : Springer, 1958 161(2023), 3 vom: 08. Nov., Seite 269-286 (DE-627)235503568 (DE-600)1398345-3 1432-119X nnns volume:161 year:2023 number:3 day:08 month:11 pages:269-286 https://dx.doi.org/10.1007/s00418-023-02249-7 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_267 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 161 2023 3 08 11 269-286 |
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10.1007/s00418-023-02249-7 doi (DE-627)SPR055002897 (SPR)s00418-023-02249-7-e DE-627 ger DE-627 rakwb eng Holota, R. verfasserin (orcid)0009-0002-5942-4441 aut Cleaved caspase-3 is present in the majority of glial cells in the intact rat spinal cord during postnatal life 2023 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s) 2023 Abstract Cell death is an essential process that occurs during the development of the central nervous system. Despite the availability of a wide range of commercially produced antibodies against various apoptotic markers, data regarding apoptosis in intact spinal cord during postnatal development and adulthood are mostly missing. We investigated apoptosis in rat spinal cord at different stages of ontogenesis (postnatal days 8, 29, and 90). For this purpose, we applied immunofluorescent detection of two widely used apoptotic markers, cleaved caspase-3 (cC3) and cleaved poly(ADP-ribose) polymerase (cPARP). Surprisingly, we found significant discrepancy between the number of $ cC3^{+} $ cells and $ PARP^{+} $ cells, with a ratio between 500:1 and 5000:1 in rat spinal cord at all postnatal time points. The majority of $ cC3^{+} $ cells were glial cells and did not exhibit an apoptotic phenotype. In contrast with in vivo results, in vitro analysis of primary cell cultures derived from neonatal rat spinal cord and treated with the apoptotic inductor staurosporine revealed a similar onset of occurrence of both cC3 and cPARP in cells subjected to apoptosis. Gene expression analysis of spinal cord revealed elevated expression of the Birc4 (XIAP), Birc2, and Birc5 (Survivin) genes, which are known potent inhibitors of apoptosis. Our data indicate that cC3 is not an exclusive marker of apoptosis, especially in glial cells, owing its possible presence in inhibited forms and/or its participation in other non-apoptotic roles. Therefore, cPARP appears to be a more appropriate marker to detect apoptosis. Apoptosis (dpeaa)DE-He213 Cleaved caspase-3 (dpeaa)DE-He213 Cleaved PARP (dpeaa)DE-He213 Spinal cord (dpeaa)DE-He213 Rat (dpeaa)DE-He213 Development (dpeaa)DE-He213 Dečmanová, V. (orcid)0000-0003-0281-5126 aut Alexovič Matiašová, A. (orcid)0000-0002-5500-4549 aut Košuth, J. (orcid)0000-0001-5584-8728 aut Slovinská, L. (orcid)0000-0002-9945-0655 aut Pačut, L. aut Tomori, Z. (orcid)0000-0002-0685-0247 aut Daxnerová, Z. (orcid)0000-0002-5778-8033 aut Ševc, J. (orcid)0000-0001-8050-4185 aut Enthalten in Histochemistry and cell biology Berlin : Springer, 1958 161(2023), 3 vom: 08. Nov., Seite 269-286 (DE-627)235503568 (DE-600)1398345-3 1432-119X nnns volume:161 year:2023 number:3 day:08 month:11 pages:269-286 https://dx.doi.org/10.1007/s00418-023-02249-7 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_267 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 161 2023 3 08 11 269-286 |
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10.1007/s00418-023-02249-7 doi (DE-627)SPR055002897 (SPR)s00418-023-02249-7-e DE-627 ger DE-627 rakwb eng Holota, R. verfasserin (orcid)0009-0002-5942-4441 aut Cleaved caspase-3 is present in the majority of glial cells in the intact rat spinal cord during postnatal life 2023 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s) 2023 Abstract Cell death is an essential process that occurs during the development of the central nervous system. Despite the availability of a wide range of commercially produced antibodies against various apoptotic markers, data regarding apoptosis in intact spinal cord during postnatal development and adulthood are mostly missing. We investigated apoptosis in rat spinal cord at different stages of ontogenesis (postnatal days 8, 29, and 90). For this purpose, we applied immunofluorescent detection of two widely used apoptotic markers, cleaved caspase-3 (cC3) and cleaved poly(ADP-ribose) polymerase (cPARP). Surprisingly, we found significant discrepancy between the number of $ cC3^{+} $ cells and $ PARP^{+} $ cells, with a ratio between 500:1 and 5000:1 in rat spinal cord at all postnatal time points. The majority of $ cC3^{+} $ cells were glial cells and did not exhibit an apoptotic phenotype. In contrast with in vivo results, in vitro analysis of primary cell cultures derived from neonatal rat spinal cord and treated with the apoptotic inductor staurosporine revealed a similar onset of occurrence of both cC3 and cPARP in cells subjected to apoptosis. Gene expression analysis of spinal cord revealed elevated expression of the Birc4 (XIAP), Birc2, and Birc5 (Survivin) genes, which are known potent inhibitors of apoptosis. Our data indicate that cC3 is not an exclusive marker of apoptosis, especially in glial cells, owing its possible presence in inhibited forms and/or its participation in other non-apoptotic roles. Therefore, cPARP appears to be a more appropriate marker to detect apoptosis. Apoptosis (dpeaa)DE-He213 Cleaved caspase-3 (dpeaa)DE-He213 Cleaved PARP (dpeaa)DE-He213 Spinal cord (dpeaa)DE-He213 Rat (dpeaa)DE-He213 Development (dpeaa)DE-He213 Dečmanová, V. (orcid)0000-0003-0281-5126 aut Alexovič Matiašová, A. (orcid)0000-0002-5500-4549 aut Košuth, J. (orcid)0000-0001-5584-8728 aut Slovinská, L. (orcid)0000-0002-9945-0655 aut Pačut, L. aut Tomori, Z. (orcid)0000-0002-0685-0247 aut Daxnerová, Z. (orcid)0000-0002-5778-8033 aut Ševc, J. (orcid)0000-0001-8050-4185 aut Enthalten in Histochemistry and cell biology Berlin : Springer, 1958 161(2023), 3 vom: 08. Nov., Seite 269-286 (DE-627)235503568 (DE-600)1398345-3 1432-119X nnns volume:161 year:2023 number:3 day:08 month:11 pages:269-286 https://dx.doi.org/10.1007/s00418-023-02249-7 kostenfrei Volltext GBV_USEFLAG_A SYSFLAG_A GBV_SPRINGER GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_267 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 161 2023 3 08 11 269-286 |
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Enthalten in Histochemistry and cell biology 161(2023), 3 vom: 08. Nov., Seite 269-286 volume:161 year:2023 number:3 day:08 month:11 pages:269-286 |
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Enthalten in Histochemistry and cell biology 161(2023), 3 vom: 08. Nov., Seite 269-286 volume:161 year:2023 number:3 day:08 month:11 pages:269-286 |
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Apoptosis Cleaved caspase-3 Cleaved PARP Spinal cord Rat Development |
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Holota, R. @@aut@@ Dečmanová, V. @@aut@@ Alexovič Matiašová, A. @@aut@@ Košuth, J. @@aut@@ Slovinská, L. @@aut@@ Pačut, L. @@aut@@ Tomori, Z. @@aut@@ Daxnerová, Z. @@aut@@ Ševc, J. @@aut@@ |
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Despite the availability of a wide range of commercially produced antibodies against various apoptotic markers, data regarding apoptosis in intact spinal cord during postnatal development and adulthood are mostly missing. We investigated apoptosis in rat spinal cord at different stages of ontogenesis (postnatal days 8, 29, and 90). For this purpose, we applied immunofluorescent detection of two widely used apoptotic markers, cleaved caspase-3 (cC3) and cleaved poly(ADP-ribose) polymerase (cPARP). Surprisingly, we found significant discrepancy between the number of $ cC3^{+} $ cells and $ PARP^{+} $ cells, with a ratio between 500:1 and 5000:1 in rat spinal cord at all postnatal time points. The majority of $ cC3^{+} $ cells were glial cells and did not exhibit an apoptotic phenotype. In contrast with in vivo results, in vitro analysis of primary cell cultures derived from neonatal rat spinal cord and treated with the apoptotic inductor staurosporine revealed a similar onset of occurrence of both cC3 and cPARP in cells subjected to apoptosis. Gene expression analysis of spinal cord revealed elevated expression of the Birc4 (XIAP), Birc2, and Birc5 (Survivin) genes, which are known potent inhibitors of apoptosis. Our data indicate that cC3 is not an exclusive marker of apoptosis, especially in glial cells, owing its possible presence in inhibited forms and/or its participation in other non-apoptotic roles. 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Holota, R. |
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Holota, R. misc Apoptosis misc Cleaved caspase-3 misc Cleaved PARP misc Spinal cord misc Rat misc Development Cleaved caspase-3 is present in the majority of glial cells in the intact rat spinal cord during postnatal life |
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Cleaved caspase-3 is present in the majority of glial cells in the intact rat spinal cord during postnatal life Apoptosis (dpeaa)DE-He213 Cleaved caspase-3 (dpeaa)DE-He213 Cleaved PARP (dpeaa)DE-He213 Spinal cord (dpeaa)DE-He213 Rat (dpeaa)DE-He213 Development (dpeaa)DE-He213 |
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Holota, R. |
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10.1007/s00418-023-02249-7 |
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(ORCID)0009-0002-5942-4441 (ORCID)0000-0003-0281-5126 (ORCID)0000-0002-5500-4549 (ORCID)0000-0001-5584-8728 (ORCID)0000-0002-9945-0655 (ORCID)0000-0002-0685-0247 (ORCID)0000-0002-5778-8033 (ORCID)0000-0001-8050-4185 |
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| title_sort |
cleaved caspase-3 is present in the majority of glial cells in the intact rat spinal cord during postnatal life |
| title_auth |
Cleaved caspase-3 is present in the majority of glial cells in the intact rat spinal cord during postnatal life |
| abstract |
Abstract Cell death is an essential process that occurs during the development of the central nervous system. Despite the availability of a wide range of commercially produced antibodies against various apoptotic markers, data regarding apoptosis in intact spinal cord during postnatal development and adulthood are mostly missing. We investigated apoptosis in rat spinal cord at different stages of ontogenesis (postnatal days 8, 29, and 90). For this purpose, we applied immunofluorescent detection of two widely used apoptotic markers, cleaved caspase-3 (cC3) and cleaved poly(ADP-ribose) polymerase (cPARP). Surprisingly, we found significant discrepancy between the number of $ cC3^{+} $ cells and $ PARP^{+} $ cells, with a ratio between 500:1 and 5000:1 in rat spinal cord at all postnatal time points. The majority of $ cC3^{+} $ cells were glial cells and did not exhibit an apoptotic phenotype. In contrast with in vivo results, in vitro analysis of primary cell cultures derived from neonatal rat spinal cord and treated with the apoptotic inductor staurosporine revealed a similar onset of occurrence of both cC3 and cPARP in cells subjected to apoptosis. Gene expression analysis of spinal cord revealed elevated expression of the Birc4 (XIAP), Birc2, and Birc5 (Survivin) genes, which are known potent inhibitors of apoptosis. Our data indicate that cC3 is not an exclusive marker of apoptosis, especially in glial cells, owing its possible presence in inhibited forms and/or its participation in other non-apoptotic roles. Therefore, cPARP appears to be a more appropriate marker to detect apoptosis. © The Author(s) 2023 |
| abstractGer |
Abstract Cell death is an essential process that occurs during the development of the central nervous system. Despite the availability of a wide range of commercially produced antibodies against various apoptotic markers, data regarding apoptosis in intact spinal cord during postnatal development and adulthood are mostly missing. We investigated apoptosis in rat spinal cord at different stages of ontogenesis (postnatal days 8, 29, and 90). For this purpose, we applied immunofluorescent detection of two widely used apoptotic markers, cleaved caspase-3 (cC3) and cleaved poly(ADP-ribose) polymerase (cPARP). Surprisingly, we found significant discrepancy between the number of $ cC3^{+} $ cells and $ PARP^{+} $ cells, with a ratio between 500:1 and 5000:1 in rat spinal cord at all postnatal time points. The majority of $ cC3^{+} $ cells were glial cells and did not exhibit an apoptotic phenotype. In contrast with in vivo results, in vitro analysis of primary cell cultures derived from neonatal rat spinal cord and treated with the apoptotic inductor staurosporine revealed a similar onset of occurrence of both cC3 and cPARP in cells subjected to apoptosis. Gene expression analysis of spinal cord revealed elevated expression of the Birc4 (XIAP), Birc2, and Birc5 (Survivin) genes, which are known potent inhibitors of apoptosis. Our data indicate that cC3 is not an exclusive marker of apoptosis, especially in glial cells, owing its possible presence in inhibited forms and/or its participation in other non-apoptotic roles. Therefore, cPARP appears to be a more appropriate marker to detect apoptosis. © The Author(s) 2023 |
| abstract_unstemmed |
Abstract Cell death is an essential process that occurs during the development of the central nervous system. Despite the availability of a wide range of commercially produced antibodies against various apoptotic markers, data regarding apoptosis in intact spinal cord during postnatal development and adulthood are mostly missing. We investigated apoptosis in rat spinal cord at different stages of ontogenesis (postnatal days 8, 29, and 90). For this purpose, we applied immunofluorescent detection of two widely used apoptotic markers, cleaved caspase-3 (cC3) and cleaved poly(ADP-ribose) polymerase (cPARP). Surprisingly, we found significant discrepancy between the number of $ cC3^{+} $ cells and $ PARP^{+} $ cells, with a ratio between 500:1 and 5000:1 in rat spinal cord at all postnatal time points. The majority of $ cC3^{+} $ cells were glial cells and did not exhibit an apoptotic phenotype. In contrast with in vivo results, in vitro analysis of primary cell cultures derived from neonatal rat spinal cord and treated with the apoptotic inductor staurosporine revealed a similar onset of occurrence of both cC3 and cPARP in cells subjected to apoptosis. Gene expression analysis of spinal cord revealed elevated expression of the Birc4 (XIAP), Birc2, and Birc5 (Survivin) genes, which are known potent inhibitors of apoptosis. Our data indicate that cC3 is not an exclusive marker of apoptosis, especially in glial cells, owing its possible presence in inhibited forms and/or its participation in other non-apoptotic roles. Therefore, cPARP appears to be a more appropriate marker to detect apoptosis. © The Author(s) 2023 |
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Cleaved caspase-3 is present in the majority of glial cells in the intact rat spinal cord during postnatal life |
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Dečmanová, V. Alexovič Matiašová, A. Košuth, J. Slovinská, L. Pačut, L. Tomori, Z. Daxnerová, Z. Ševc, J. |
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Dečmanová, V. Alexovič Matiašová, A. Košuth, J. Slovinská, L. Pačut, L. Tomori, Z. Daxnerová, Z. Ševc, J. |
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2024-07-04T03:48:57.159Z |
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| score |
7.400194 |