Systematic optimization and evaluation of culture conditions for the construction of circulating tumor cell clusters using breast cancer cell lines

Background Circulating tumor cell (CTC) clusters play a critical role in carcinoma metastasis. However, the rarity of CTC clusters and the limitations of capture techniques have retarded the research progress. In vitro CTC clusters model can help to further understand the biological properties of CT...
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Autor*in:	Zou, Jueyao [verfasserIn] Chen, Qiong [verfasserIn] He, Yong [verfasserIn] Pan, Yanhong [verfasserIn] Zhao, Han [verfasserIn] Shi, Junfeng [verfasserIn] Wei, Zhonghong [verfasserIn] Yu, Suyun [verfasserIn] Zhao, Yang [verfasserIn] Han, Xin [verfasserIn] Lu, Yin [verfasserIn] Chen, Wenxing [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2024

Schlagwörter:	Circulating tumor cell clusters Tumor metastasis Culture methods Molecular characteristics

Anmerkung:	© The Author(s) 2024

Übergeordnetes Werk:	Enthalten in: BMC cancer - BioMed Central, 2001, 24(2024), 1 vom: 23. Apr.
Übergeordnetes Werk:	volume:24 ; year:2024 ; number:1 ; day:23 ; month:04

Links:	Volltext

DOI / URN:	10.1186/s12885-024-12214-9

Katalog-ID:	SPR055619495

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520			\|a Background Circulating tumor cell (CTC) clusters play a critical role in carcinoma metastasis. However, the rarity of CTC clusters and the limitations of capture techniques have retarded the research progress. In vitro CTC clusters model can help to further understand the biological properties of CTC clusters and their clinical significance. Therefore, it is necessary to establish reliable in vitro methodological models to form CTC clusters whose biological characteristics are very similar to clinical CTC clusters. Methods The assays of immunofluorescence, transmission electron microscopy, EdU incorporation, cell adhension and microfluidic chips were used. The experimental metastasis model in mice was used. Results We systematically optimized the culture methods to form in vitro CTC clusters model, and more importantly, evaluated it with reference to the biological capabilities of reported clinical CTC clusters. In vitro CTC clusters exhibited a high degree of similarity to the reported pathological characteristics of CTC clusters isolated from patients at different stages of tumor metastasis, including the appearance morphology, size, adhesive and tight junctions-associated proteins, and other indicators of CTC clusters. Furthermore, in vivo experiments also demonstrated that the CTC clusters had an enhanced ability to grow and metastasize compared to single CTC. Conclusions The study provides a reliable model to help to obtain comparatively stable and qualified CTC clusters in vitro, propelling the studies on tumor metastasis.
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allfields	10.1186/s12885-024-12214-9 doi (DE-627)SPR055619495 (SPR)s12885-024-12214-9-e DE-627 ger DE-627 rakwb eng 610 VZ 44.00 bkl Zou, Jueyao verfasserin aut Systematic optimization and evaluation of culture conditions for the construction of circulating tumor cell clusters using breast cancer cell lines 2024 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s) 2024 Background Circulating tumor cell (CTC) clusters play a critical role in carcinoma metastasis. However, the rarity of CTC clusters and the limitations of capture techniques have retarded the research progress. In vitro CTC clusters model can help to further understand the biological properties of CTC clusters and their clinical significance. Therefore, it is necessary to establish reliable in vitro methodological models to form CTC clusters whose biological characteristics are very similar to clinical CTC clusters. Methods The assays of immunofluorescence, transmission electron microscopy, EdU incorporation, cell adhension and microfluidic chips were used. The experimental metastasis model in mice was used. Results We systematically optimized the culture methods to form in vitro CTC clusters model, and more importantly, evaluated it with reference to the biological capabilities of reported clinical CTC clusters. In vitro CTC clusters exhibited a high degree of similarity to the reported pathological characteristics of CTC clusters isolated from patients at different stages of tumor metastasis, including the appearance morphology, size, adhesive and tight junctions-associated proteins, and other indicators of CTC clusters. Furthermore, in vivo experiments also demonstrated that the CTC clusters had an enhanced ability to grow and metastasize compared to single CTC. Conclusions The study provides a reliable model to help to obtain comparatively stable and qualified CTC clusters in vitro, propelling the studies on tumor metastasis. Circulating tumor cell clusters (dpeaa)DE-He213 Tumor metastasis (dpeaa)DE-He213 Culture methods (dpeaa)DE-He213 Molecular characteristics (dpeaa)DE-He213 Chen, Qiong verfasserin aut He, Yong verfasserin aut Pan, Yanhong verfasserin aut Zhao, Han verfasserin aut Shi, Junfeng verfasserin aut Wei, Zhonghong verfasserin aut Yu, Suyun verfasserin aut Zhao, Yang verfasserin aut Han, Xin verfasserin aut Lu, Yin verfasserin aut Chen, Wenxing verfasserin aut Enthalten in BMC cancer BioMed Central, 2001 24(2024), 1 vom: 23. Apr. (DE-627)326643710 (DE-600)2041352-X 1471-2407 nnns volume:24 year:2024 number:1 day:23 month:04 https://dx.doi.org/10.1186/s12885-024-12214-9 X:SPRINGER Resolving-System kostenfrei Volltext SYSFLAG_0 GBV_SPRINGER SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 44.00 VZ AR 24 2024 1 23 04
spelling	10.1186/s12885-024-12214-9 doi (DE-627)SPR055619495 (SPR)s12885-024-12214-9-e DE-627 ger DE-627 rakwb eng 610 VZ 44.00 bkl Zou, Jueyao verfasserin aut Systematic optimization and evaluation of culture conditions for the construction of circulating tumor cell clusters using breast cancer cell lines 2024 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s) 2024 Background Circulating tumor cell (CTC) clusters play a critical role in carcinoma metastasis. However, the rarity of CTC clusters and the limitations of capture techniques have retarded the research progress. In vitro CTC clusters model can help to further understand the biological properties of CTC clusters and their clinical significance. Therefore, it is necessary to establish reliable in vitro methodological models to form CTC clusters whose biological characteristics are very similar to clinical CTC clusters. Methods The assays of immunofluorescence, transmission electron microscopy, EdU incorporation, cell adhension and microfluidic chips were used. The experimental metastasis model in mice was used. Results We systematically optimized the culture methods to form in vitro CTC clusters model, and more importantly, evaluated it with reference to the biological capabilities of reported clinical CTC clusters. In vitro CTC clusters exhibited a high degree of similarity to the reported pathological characteristics of CTC clusters isolated from patients at different stages of tumor metastasis, including the appearance morphology, size, adhesive and tight junctions-associated proteins, and other indicators of CTC clusters. Furthermore, in vivo experiments also demonstrated that the CTC clusters had an enhanced ability to grow and metastasize compared to single CTC. Conclusions The study provides a reliable model to help to obtain comparatively stable and qualified CTC clusters in vitro, propelling the studies on tumor metastasis. Circulating tumor cell clusters (dpeaa)DE-He213 Tumor metastasis (dpeaa)DE-He213 Culture methods (dpeaa)DE-He213 Molecular characteristics (dpeaa)DE-He213 Chen, Qiong verfasserin aut He, Yong verfasserin aut Pan, Yanhong verfasserin aut Zhao, Han verfasserin aut Shi, Junfeng verfasserin aut Wei, Zhonghong verfasserin aut Yu, Suyun verfasserin aut Zhao, Yang verfasserin aut Han, Xin verfasserin aut Lu, Yin verfasserin aut Chen, Wenxing verfasserin aut Enthalten in BMC cancer BioMed Central, 2001 24(2024), 1 vom: 23. Apr. (DE-627)326643710 (DE-600)2041352-X 1471-2407 nnns volume:24 year:2024 number:1 day:23 month:04 https://dx.doi.org/10.1186/s12885-024-12214-9 X:SPRINGER Resolving-System kostenfrei Volltext SYSFLAG_0 GBV_SPRINGER SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 44.00 VZ AR 24 2024 1 23 04
allfields_unstemmed	10.1186/s12885-024-12214-9 doi (DE-627)SPR055619495 (SPR)s12885-024-12214-9-e DE-627 ger DE-627 rakwb eng 610 VZ 44.00 bkl Zou, Jueyao verfasserin aut Systematic optimization and evaluation of culture conditions for the construction of circulating tumor cell clusters using breast cancer cell lines 2024 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s) 2024 Background Circulating tumor cell (CTC) clusters play a critical role in carcinoma metastasis. However, the rarity of CTC clusters and the limitations of capture techniques have retarded the research progress. In vitro CTC clusters model can help to further understand the biological properties of CTC clusters and their clinical significance. Therefore, it is necessary to establish reliable in vitro methodological models to form CTC clusters whose biological characteristics are very similar to clinical CTC clusters. Methods The assays of immunofluorescence, transmission electron microscopy, EdU incorporation, cell adhension and microfluidic chips were used. The experimental metastasis model in mice was used. Results We systematically optimized the culture methods to form in vitro CTC clusters model, and more importantly, evaluated it with reference to the biological capabilities of reported clinical CTC clusters. In vitro CTC clusters exhibited a high degree of similarity to the reported pathological characteristics of CTC clusters isolated from patients at different stages of tumor metastasis, including the appearance morphology, size, adhesive and tight junctions-associated proteins, and other indicators of CTC clusters. Furthermore, in vivo experiments also demonstrated that the CTC clusters had an enhanced ability to grow and metastasize compared to single CTC. Conclusions The study provides a reliable model to help to obtain comparatively stable and qualified CTC clusters in vitro, propelling the studies on tumor metastasis. Circulating tumor cell clusters (dpeaa)DE-He213 Tumor metastasis (dpeaa)DE-He213 Culture methods (dpeaa)DE-He213 Molecular characteristics (dpeaa)DE-He213 Chen, Qiong verfasserin aut He, Yong verfasserin aut Pan, Yanhong verfasserin aut Zhao, Han verfasserin aut Shi, Junfeng verfasserin aut Wei, Zhonghong verfasserin aut Yu, Suyun verfasserin aut Zhao, Yang verfasserin aut Han, Xin verfasserin aut Lu, Yin verfasserin aut Chen, Wenxing verfasserin aut Enthalten in BMC cancer BioMed Central, 2001 24(2024), 1 vom: 23. Apr. (DE-627)326643710 (DE-600)2041352-X 1471-2407 nnns volume:24 year:2024 number:1 day:23 month:04 https://dx.doi.org/10.1186/s12885-024-12214-9 X:SPRINGER Resolving-System kostenfrei Volltext SYSFLAG_0 GBV_SPRINGER SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 44.00 VZ AR 24 2024 1 23 04
allfieldsGer	10.1186/s12885-024-12214-9 doi (DE-627)SPR055619495 (SPR)s12885-024-12214-9-e DE-627 ger DE-627 rakwb eng 610 VZ 44.00 bkl Zou, Jueyao verfasserin aut Systematic optimization and evaluation of culture conditions for the construction of circulating tumor cell clusters using breast cancer cell lines 2024 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s) 2024 Background Circulating tumor cell (CTC) clusters play a critical role in carcinoma metastasis. However, the rarity of CTC clusters and the limitations of capture techniques have retarded the research progress. In vitro CTC clusters model can help to further understand the biological properties of CTC clusters and their clinical significance. Therefore, it is necessary to establish reliable in vitro methodological models to form CTC clusters whose biological characteristics are very similar to clinical CTC clusters. Methods The assays of immunofluorescence, transmission electron microscopy, EdU incorporation, cell adhension and microfluidic chips were used. The experimental metastasis model in mice was used. Results We systematically optimized the culture methods to form in vitro CTC clusters model, and more importantly, evaluated it with reference to the biological capabilities of reported clinical CTC clusters. In vitro CTC clusters exhibited a high degree of similarity to the reported pathological characteristics of CTC clusters isolated from patients at different stages of tumor metastasis, including the appearance morphology, size, adhesive and tight junctions-associated proteins, and other indicators of CTC clusters. Furthermore, in vivo experiments also demonstrated that the CTC clusters had an enhanced ability to grow and metastasize compared to single CTC. Conclusions The study provides a reliable model to help to obtain comparatively stable and qualified CTC clusters in vitro, propelling the studies on tumor metastasis. Circulating tumor cell clusters (dpeaa)DE-He213 Tumor metastasis (dpeaa)DE-He213 Culture methods (dpeaa)DE-He213 Molecular characteristics (dpeaa)DE-He213 Chen, Qiong verfasserin aut He, Yong verfasserin aut Pan, Yanhong verfasserin aut Zhao, Han verfasserin aut Shi, Junfeng verfasserin aut Wei, Zhonghong verfasserin aut Yu, Suyun verfasserin aut Zhao, Yang verfasserin aut Han, Xin verfasserin aut Lu, Yin verfasserin aut Chen, Wenxing verfasserin aut Enthalten in BMC cancer BioMed Central, 2001 24(2024), 1 vom: 23. Apr. (DE-627)326643710 (DE-600)2041352-X 1471-2407 nnns volume:24 year:2024 number:1 day:23 month:04 https://dx.doi.org/10.1186/s12885-024-12214-9 X:SPRINGER Resolving-System kostenfrei Volltext SYSFLAG_0 GBV_SPRINGER SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 44.00 VZ AR 24 2024 1 23 04
allfieldsSound	10.1186/s12885-024-12214-9 doi (DE-627)SPR055619495 (SPR)s12885-024-12214-9-e DE-627 ger DE-627 rakwb eng 610 VZ 44.00 bkl Zou, Jueyao verfasserin aut Systematic optimization and evaluation of culture conditions for the construction of circulating tumor cell clusters using breast cancer cell lines 2024 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s) 2024 Background Circulating tumor cell (CTC) clusters play a critical role in carcinoma metastasis. However, the rarity of CTC clusters and the limitations of capture techniques have retarded the research progress. In vitro CTC clusters model can help to further understand the biological properties of CTC clusters and their clinical significance. Therefore, it is necessary to establish reliable in vitro methodological models to form CTC clusters whose biological characteristics are very similar to clinical CTC clusters. Methods The assays of immunofluorescence, transmission electron microscopy, EdU incorporation, cell adhension and microfluidic chips were used. The experimental metastasis model in mice was used. Results We systematically optimized the culture methods to form in vitro CTC clusters model, and more importantly, evaluated it with reference to the biological capabilities of reported clinical CTC clusters. In vitro CTC clusters exhibited a high degree of similarity to the reported pathological characteristics of CTC clusters isolated from patients at different stages of tumor metastasis, including the appearance morphology, size, adhesive and tight junctions-associated proteins, and other indicators of CTC clusters. Furthermore, in vivo experiments also demonstrated that the CTC clusters had an enhanced ability to grow and metastasize compared to single CTC. Conclusions The study provides a reliable model to help to obtain comparatively stable and qualified CTC clusters in vitro, propelling the studies on tumor metastasis. Circulating tumor cell clusters (dpeaa)DE-He213 Tumor metastasis (dpeaa)DE-He213 Culture methods (dpeaa)DE-He213 Molecular characteristics (dpeaa)DE-He213 Chen, Qiong verfasserin aut He, Yong verfasserin aut Pan, Yanhong verfasserin aut Zhao, Han verfasserin aut Shi, Junfeng verfasserin aut Wei, Zhonghong verfasserin aut Yu, Suyun verfasserin aut Zhao, Yang verfasserin aut Han, Xin verfasserin aut Lu, Yin verfasserin aut Chen, Wenxing verfasserin aut Enthalten in BMC cancer BioMed Central, 2001 24(2024), 1 vom: 23. Apr. (DE-627)326643710 (DE-600)2041352-X 1471-2407 nnns volume:24 year:2024 number:1 day:23 month:04 https://dx.doi.org/10.1186/s12885-024-12214-9 X:SPRINGER Resolving-System kostenfrei Volltext SYSFLAG_0 GBV_SPRINGER SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 44.00 VZ AR 24 2024 1 23 04
language	English
source	Enthalten in BMC cancer 24(2024), 1 vom: 23. Apr. volume:24 year:2024 number:1 day:23 month:04
sourceStr	Enthalten in BMC cancer 24(2024), 1 vom: 23. Apr. volume:24 year:2024 number:1 day:23 month:04
format_phy_str_mv	Article
institution	findex.gbv.de
topic_facet	Circulating tumor cell clusters Tumor metastasis Culture methods Molecular characteristics
dewey-raw	610
isfreeaccess_bool	true
container_title	BMC cancer
authorswithroles_txt_mv	Zou, Jueyao @@aut@@ Chen, Qiong @@aut@@ He, Yong @@aut@@ Pan, Yanhong @@aut@@ Zhao, Han @@aut@@ Shi, Junfeng @@aut@@ Wei, Zhonghong @@aut@@ Yu, Suyun @@aut@@ Zhao, Yang @@aut@@ Han, Xin @@aut@@ Lu, Yin @@aut@@ Chen, Wenxing @@aut@@
publishDateDaySort_date	2024-04-23T00:00:00Z
hierarchy_top_id	326643710
dewey-sort	3610
id	SPR055619495
language_de	englisch
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However, the rarity of CTC clusters and the limitations of capture techniques have retarded the research progress. In vitro CTC clusters model can help to further understand the biological properties of CTC clusters and their clinical significance. Therefore, it is necessary to establish reliable in vitro methodological models to form CTC clusters whose biological characteristics are very similar to clinical CTC clusters. Methods The assays of immunofluorescence, transmission electron microscopy, EdU incorporation, cell adhension and microfluidic chips were used. The experimental metastasis model in mice was used. Results We systematically optimized the culture methods to form in vitro CTC clusters model, and more importantly, evaluated it with reference to the biological capabilities of reported clinical CTC clusters. In vitro CTC clusters exhibited a high degree of similarity to the reported pathological characteristics of CTC clusters isolated from patients at different stages of tumor metastasis, including the appearance morphology, size, adhesive and tight junctions-associated proteins, and other indicators of CTC clusters. Furthermore, in vivo experiments also demonstrated that the CTC clusters had an enhanced ability to grow and metastasize compared to single CTC. 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author	Zou, Jueyao
spellingShingle	Zou, Jueyao ddc 610 bkl 44.00 misc Circulating tumor cell clusters misc Tumor metastasis misc Culture methods misc Molecular characteristics Systematic optimization and evaluation of culture conditions for the construction of circulating tumor cell clusters using breast cancer cell lines
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topic_title	610 VZ 44.00 bkl Systematic optimization and evaluation of culture conditions for the construction of circulating tumor cell clusters using breast cancer cell lines Circulating tumor cell clusters (dpeaa)DE-He213 Tumor metastasis (dpeaa)DE-He213 Culture methods (dpeaa)DE-He213 Molecular characteristics (dpeaa)DE-He213
topic	ddc 610 bkl 44.00 misc Circulating tumor cell clusters misc Tumor metastasis misc Culture methods misc Molecular characteristics
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title	Systematic optimization and evaluation of culture conditions for the construction of circulating tumor cell clusters using breast cancer cell lines
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title_full	Systematic optimization and evaluation of culture conditions for the construction of circulating tumor cell clusters using breast cancer cell lines
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author_browse	Zou, Jueyao Chen, Qiong He, Yong Pan, Yanhong Zhao, Han Shi, Junfeng Wei, Zhonghong Yu, Suyun Zhao, Yang Han, Xin Lu, Yin Chen, Wenxing
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title_sort	systematic optimization and evaluation of culture conditions for the construction of circulating tumor cell clusters using breast cancer cell lines
title_auth	Systematic optimization and evaluation of culture conditions for the construction of circulating tumor cell clusters using breast cancer cell lines
abstract	Background Circulating tumor cell (CTC) clusters play a critical role in carcinoma metastasis. However, the rarity of CTC clusters and the limitations of capture techniques have retarded the research progress. In vitro CTC clusters model can help to further understand the biological properties of CTC clusters and their clinical significance. Therefore, it is necessary to establish reliable in vitro methodological models to form CTC clusters whose biological characteristics are very similar to clinical CTC clusters. Methods The assays of immunofluorescence, transmission electron microscopy, EdU incorporation, cell adhension and microfluidic chips were used. The experimental metastasis model in mice was used. Results We systematically optimized the culture methods to form in vitro CTC clusters model, and more importantly, evaluated it with reference to the biological capabilities of reported clinical CTC clusters. In vitro CTC clusters exhibited a high degree of similarity to the reported pathological characteristics of CTC clusters isolated from patients at different stages of tumor metastasis, including the appearance morphology, size, adhesive and tight junctions-associated proteins, and other indicators of CTC clusters. Furthermore, in vivo experiments also demonstrated that the CTC clusters had an enhanced ability to grow and metastasize compared to single CTC. Conclusions The study provides a reliable model to help to obtain comparatively stable and qualified CTC clusters in vitro, propelling the studies on tumor metastasis. © The Author(s) 2024
abstractGer	Background Circulating tumor cell (CTC) clusters play a critical role in carcinoma metastasis. However, the rarity of CTC clusters and the limitations of capture techniques have retarded the research progress. In vitro CTC clusters model can help to further understand the biological properties of CTC clusters and their clinical significance. Therefore, it is necessary to establish reliable in vitro methodological models to form CTC clusters whose biological characteristics are very similar to clinical CTC clusters. Methods The assays of immunofluorescence, transmission electron microscopy, EdU incorporation, cell adhension and microfluidic chips were used. The experimental metastasis model in mice was used. Results We systematically optimized the culture methods to form in vitro CTC clusters model, and more importantly, evaluated it with reference to the biological capabilities of reported clinical CTC clusters. In vitro CTC clusters exhibited a high degree of similarity to the reported pathological characteristics of CTC clusters isolated from patients at different stages of tumor metastasis, including the appearance morphology, size, adhesive and tight junctions-associated proteins, and other indicators of CTC clusters. Furthermore, in vivo experiments also demonstrated that the CTC clusters had an enhanced ability to grow and metastasize compared to single CTC. Conclusions The study provides a reliable model to help to obtain comparatively stable and qualified CTC clusters in vitro, propelling the studies on tumor metastasis. © The Author(s) 2024
abstract_unstemmed	Background Circulating tumor cell (CTC) clusters play a critical role in carcinoma metastasis. However, the rarity of CTC clusters and the limitations of capture techniques have retarded the research progress. In vitro CTC clusters model can help to further understand the biological properties of CTC clusters and their clinical significance. Therefore, it is necessary to establish reliable in vitro methodological models to form CTC clusters whose biological characteristics are very similar to clinical CTC clusters. Methods The assays of immunofluorescence, transmission electron microscopy, EdU incorporation, cell adhension and microfluidic chips were used. The experimental metastasis model in mice was used. Results We systematically optimized the culture methods to form in vitro CTC clusters model, and more importantly, evaluated it with reference to the biological capabilities of reported clinical CTC clusters. In vitro CTC clusters exhibited a high degree of similarity to the reported pathological characteristics of CTC clusters isolated from patients at different stages of tumor metastasis, including the appearance morphology, size, adhesive and tight junctions-associated proteins, and other indicators of CTC clusters. Furthermore, in vivo experiments also demonstrated that the CTC clusters had an enhanced ability to grow and metastasize compared to single CTC. Conclusions The study provides a reliable model to help to obtain comparatively stable and qualified CTC clusters in vitro, propelling the studies on tumor metastasis. © The Author(s) 2024
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title_short	Systematic optimization and evaluation of culture conditions for the construction of circulating tumor cell clusters using breast cancer cell lines
url	https://dx.doi.org/10.1186/s12885-024-12214-9
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author2	Chen, Qiong He, Yong Pan, Yanhong Zhao, Han Shi, Junfeng Wei, Zhonghong Yu, Suyun Zhao, Yang Han, Xin Lu, Yin Chen, Wenxing
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up_date	2024-07-03T16:53:43.938Z
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In vitro CTC clusters exhibited a high degree of similarity to the reported pathological characteristics of CTC clusters isolated from patients at different stages of tumor metastasis, including the appearance morphology, size, adhesive and tight junctions-associated proteins, and other indicators of CTC clusters. Furthermore, in vivo experiments also demonstrated that the CTC clusters had an enhanced ability to grow and metastasize compared to single CTC. 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Systematic optimization and evaluation of culture conditions for the construction of circulating tumor cell clusters using breast cancer cell lines

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