Case study observational research: inflammatory cytokines in the bronchial epithelial lining fluid of COVID-19 patients with acute hypoxemic respiratory failure
Background In this study, the concentrations of inflammatory cytokines were measured in the bronchial epithelial lining fluid (ELF) and plasma in patients with acute hypoxemic respiratory failure (AHRF) secondary to severe coronavirus disease 2019 (COVID-19). Methods We comprehensively analyzed the...
Ausführliche Beschreibung
Autor*in: |
Sudo, Kazuki [verfasserIn] Kinoshita, Mao [verfasserIn] Kawaguchi, Ken [verfasserIn] Kushimoto, Kohsuke [verfasserIn] Yoshii, Ryogo [verfasserIn] Inoue, Keita [verfasserIn] Yamasaki, Masaki [verfasserIn] Matsuyama, Tasuku [verfasserIn] Kooguchi, Kunihiko [verfasserIn] Takashima, Yasuo [verfasserIn] Tanaka, Masami [verfasserIn] Matsumoto, Kazumichi [verfasserIn] Tashiro, Kei [verfasserIn] Inaba, Tohru [verfasserIn] Ohta, Bon [verfasserIn] Sawa, Teiji [verfasserIn] |
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E-Artikel |
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Englisch |
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2024 |
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Anmerkung: |
© The Author(s) 2024 |
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Übergeordnetes Werk: |
Enthalten in: Critical care - BioMed Central, 1997, 28(2024), 1 vom: 23. Apr. |
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Übergeordnetes Werk: |
volume:28 ; year:2024 ; number:1 ; day:23 ; month:04 |
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DOI / URN: |
10.1186/s13054-024-04921-3 |
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Katalog-ID: |
SPR055620892 |
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520 | |a Background In this study, the concentrations of inflammatory cytokines were measured in the bronchial epithelial lining fluid (ELF) and plasma in patients with acute hypoxemic respiratory failure (AHRF) secondary to severe coronavirus disease 2019 (COVID-19). Methods We comprehensively analyzed the concentrations of 25 cytokines in the ELF and plasma of 27 COVID-19 AHRF patients. ELF was collected using the bronchial microsampling method through an endotracheal tube just after patients were intubated for mechanical ventilation. Results Compared with those in healthy volunteers, the concentrations of interleukin (IL)-6 (median 27.6 pmol/L), IL-8 (1045.1 pmol/L), IL-17A (0.8 pmol/L), IL-25 (1.5 pmol/L), and IL-31 (42.3 pmol/L) were significantly greater in the ELF of COVID-19 patients than in that of volunteers. The concentrations of MCP-1 and MIP-1β were significantly greater in the plasma of COVID-19 patients than in that of volunteers. The ELF/plasma ratio of IL-8 was the highest among the 25 cytokines, with a median of 737, and the ELF/plasma ratio of IL-6 (median: 218), IL-1β (202), IL-31 (169), MCP-1 (81), MIP-1β (55), and TNF-α (47) were lower. Conclusions The ELF concentrations of IL-6, IL-8, IL-17A, IL-25, and IL-31 were significantly increased in COVID-19 patients. Although high levels of MIP-1 and MIP-1β were also detected in the blood samples collected simultaneously with the ELF samples, the results indicated that lung inflammation was highly compartmentalized. Our study demonstrated that a comprehensive analysis of cytokines in the ELF is a feasible approach for understanding lung inflammation and systemic interactions in patients with severe pneumonia. | ||
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700 | 1 | |a Kinoshita, Mao |e verfasserin |4 aut | |
700 | 1 | |a Kawaguchi, Ken |e verfasserin |4 aut | |
700 | 1 | |a Kushimoto, Kohsuke |e verfasserin |4 aut | |
700 | 1 | |a Yoshii, Ryogo |e verfasserin |4 aut | |
700 | 1 | |a Inoue, Keita |e verfasserin |4 aut | |
700 | 1 | |a Yamasaki, Masaki |e verfasserin |4 aut | |
700 | 1 | |a Matsuyama, Tasuku |e verfasserin |4 aut | |
700 | 1 | |a Kooguchi, Kunihiko |e verfasserin |4 aut | |
700 | 1 | |a Takashima, Yasuo |e verfasserin |4 aut | |
700 | 1 | |a Tanaka, Masami |e verfasserin |4 aut | |
700 | 1 | |a Matsumoto, Kazumichi |e verfasserin |4 aut | |
700 | 1 | |a Tashiro, Kei |e verfasserin |4 aut | |
700 | 1 | |a Inaba, Tohru |e verfasserin |4 aut | |
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700 | 1 | |a Sawa, Teiji |e verfasserin |4 aut | |
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10.1186/s13054-024-04921-3 doi (DE-627)SPR055620892 (SPR)s13054-024-04921-3-e DE-627 ger DE-627 rakwb eng 610 VZ 44.00 bkl Sudo, Kazuki verfasserin aut Case study observational research: inflammatory cytokines in the bronchial epithelial lining fluid of COVID-19 patients with acute hypoxemic respiratory failure 2024 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s) 2024 Background In this study, the concentrations of inflammatory cytokines were measured in the bronchial epithelial lining fluid (ELF) and plasma in patients with acute hypoxemic respiratory failure (AHRF) secondary to severe coronavirus disease 2019 (COVID-19). Methods We comprehensively analyzed the concentrations of 25 cytokines in the ELF and plasma of 27 COVID-19 AHRF patients. ELF was collected using the bronchial microsampling method through an endotracheal tube just after patients were intubated for mechanical ventilation. Results Compared with those in healthy volunteers, the concentrations of interleukin (IL)-6 (median 27.6 pmol/L), IL-8 (1045.1 pmol/L), IL-17A (0.8 pmol/L), IL-25 (1.5 pmol/L), and IL-31 (42.3 pmol/L) were significantly greater in the ELF of COVID-19 patients than in that of volunteers. The concentrations of MCP-1 and MIP-1β were significantly greater in the plasma of COVID-19 patients than in that of volunteers. The ELF/plasma ratio of IL-8 was the highest among the 25 cytokines, with a median of 737, and the ELF/plasma ratio of IL-6 (median: 218), IL-1β (202), IL-31 (169), MCP-1 (81), MIP-1β (55), and TNF-α (47) were lower. Conclusions The ELF concentrations of IL-6, IL-8, IL-17A, IL-25, and IL-31 were significantly increased in COVID-19 patients. Although high levels of MIP-1 and MIP-1β were also detected in the blood samples collected simultaneously with the ELF samples, the results indicated that lung inflammation was highly compartmentalized. Our study demonstrated that a comprehensive analysis of cytokines in the ELF is a feasible approach for understanding lung inflammation and systemic interactions in patients with severe pneumonia. Bronchial microsampling (dpeaa)DE-He213 COVID-19 (dpeaa)DE-He213 Cytokine (dpeaa)DE-He213 Epithelial lining fluid (dpeaa)DE-He213 Multiplex bead-based assay (dpeaa)DE-He213 Kinoshita, Mao verfasserin aut Kawaguchi, Ken verfasserin aut Kushimoto, Kohsuke verfasserin aut Yoshii, Ryogo verfasserin aut Inoue, Keita verfasserin aut Yamasaki, Masaki verfasserin aut Matsuyama, Tasuku verfasserin aut Kooguchi, Kunihiko verfasserin aut Takashima, Yasuo verfasserin aut Tanaka, Masami verfasserin aut Matsumoto, Kazumichi verfasserin aut Tashiro, Kei verfasserin aut Inaba, Tohru verfasserin aut Ohta, Bon verfasserin aut Sawa, Teiji verfasserin aut Enthalten in Critical care BioMed Central, 1997 28(2024), 1 vom: 23. Apr. (DE-627)331258269 (DE-600)2051256-9 1364-8535 nnns volume:28 year:2024 number:1 day:23 month:04 https://dx.doi.org/10.1186/s13054-024-04921-3 X:SPRINGER Resolving-System kostenfrei Volltext SYSFLAG_0 GBV_SPRINGER SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 44.00 VZ AR 28 2024 1 23 04 |
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10.1186/s13054-024-04921-3 doi (DE-627)SPR055620892 (SPR)s13054-024-04921-3-e DE-627 ger DE-627 rakwb eng 610 VZ 44.00 bkl Sudo, Kazuki verfasserin aut Case study observational research: inflammatory cytokines in the bronchial epithelial lining fluid of COVID-19 patients with acute hypoxemic respiratory failure 2024 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s) 2024 Background In this study, the concentrations of inflammatory cytokines were measured in the bronchial epithelial lining fluid (ELF) and plasma in patients with acute hypoxemic respiratory failure (AHRF) secondary to severe coronavirus disease 2019 (COVID-19). Methods We comprehensively analyzed the concentrations of 25 cytokines in the ELF and plasma of 27 COVID-19 AHRF patients. ELF was collected using the bronchial microsampling method through an endotracheal tube just after patients were intubated for mechanical ventilation. Results Compared with those in healthy volunteers, the concentrations of interleukin (IL)-6 (median 27.6 pmol/L), IL-8 (1045.1 pmol/L), IL-17A (0.8 pmol/L), IL-25 (1.5 pmol/L), and IL-31 (42.3 pmol/L) were significantly greater in the ELF of COVID-19 patients than in that of volunteers. The concentrations of MCP-1 and MIP-1β were significantly greater in the plasma of COVID-19 patients than in that of volunteers. The ELF/plasma ratio of IL-8 was the highest among the 25 cytokines, with a median of 737, and the ELF/plasma ratio of IL-6 (median: 218), IL-1β (202), IL-31 (169), MCP-1 (81), MIP-1β (55), and TNF-α (47) were lower. Conclusions The ELF concentrations of IL-6, IL-8, IL-17A, IL-25, and IL-31 were significantly increased in COVID-19 patients. Although high levels of MIP-1 and MIP-1β were also detected in the blood samples collected simultaneously with the ELF samples, the results indicated that lung inflammation was highly compartmentalized. Our study demonstrated that a comprehensive analysis of cytokines in the ELF is a feasible approach for understanding lung inflammation and systemic interactions in patients with severe pneumonia. Bronchial microsampling (dpeaa)DE-He213 COVID-19 (dpeaa)DE-He213 Cytokine (dpeaa)DE-He213 Epithelial lining fluid (dpeaa)DE-He213 Multiplex bead-based assay (dpeaa)DE-He213 Kinoshita, Mao verfasserin aut Kawaguchi, Ken verfasserin aut Kushimoto, Kohsuke verfasserin aut Yoshii, Ryogo verfasserin aut Inoue, Keita verfasserin aut Yamasaki, Masaki verfasserin aut Matsuyama, Tasuku verfasserin aut Kooguchi, Kunihiko verfasserin aut Takashima, Yasuo verfasserin aut Tanaka, Masami verfasserin aut Matsumoto, Kazumichi verfasserin aut Tashiro, Kei verfasserin aut Inaba, Tohru verfasserin aut Ohta, Bon verfasserin aut Sawa, Teiji verfasserin aut Enthalten in Critical care BioMed Central, 1997 28(2024), 1 vom: 23. Apr. (DE-627)331258269 (DE-600)2051256-9 1364-8535 nnns volume:28 year:2024 number:1 day:23 month:04 https://dx.doi.org/10.1186/s13054-024-04921-3 X:SPRINGER Resolving-System kostenfrei Volltext SYSFLAG_0 GBV_SPRINGER SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 44.00 VZ AR 28 2024 1 23 04 |
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10.1186/s13054-024-04921-3 doi (DE-627)SPR055620892 (SPR)s13054-024-04921-3-e DE-627 ger DE-627 rakwb eng 610 VZ 44.00 bkl Sudo, Kazuki verfasserin aut Case study observational research: inflammatory cytokines in the bronchial epithelial lining fluid of COVID-19 patients with acute hypoxemic respiratory failure 2024 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s) 2024 Background In this study, the concentrations of inflammatory cytokines were measured in the bronchial epithelial lining fluid (ELF) and plasma in patients with acute hypoxemic respiratory failure (AHRF) secondary to severe coronavirus disease 2019 (COVID-19). Methods We comprehensively analyzed the concentrations of 25 cytokines in the ELF and plasma of 27 COVID-19 AHRF patients. ELF was collected using the bronchial microsampling method through an endotracheal tube just after patients were intubated for mechanical ventilation. Results Compared with those in healthy volunteers, the concentrations of interleukin (IL)-6 (median 27.6 pmol/L), IL-8 (1045.1 pmol/L), IL-17A (0.8 pmol/L), IL-25 (1.5 pmol/L), and IL-31 (42.3 pmol/L) were significantly greater in the ELF of COVID-19 patients than in that of volunteers. The concentrations of MCP-1 and MIP-1β were significantly greater in the plasma of COVID-19 patients than in that of volunteers. The ELF/plasma ratio of IL-8 was the highest among the 25 cytokines, with a median of 737, and the ELF/plasma ratio of IL-6 (median: 218), IL-1β (202), IL-31 (169), MCP-1 (81), MIP-1β (55), and TNF-α (47) were lower. Conclusions The ELF concentrations of IL-6, IL-8, IL-17A, IL-25, and IL-31 were significantly increased in COVID-19 patients. Although high levels of MIP-1 and MIP-1β were also detected in the blood samples collected simultaneously with the ELF samples, the results indicated that lung inflammation was highly compartmentalized. Our study demonstrated that a comprehensive analysis of cytokines in the ELF is a feasible approach for understanding lung inflammation and systemic interactions in patients with severe pneumonia. Bronchial microsampling (dpeaa)DE-He213 COVID-19 (dpeaa)DE-He213 Cytokine (dpeaa)DE-He213 Epithelial lining fluid (dpeaa)DE-He213 Multiplex bead-based assay (dpeaa)DE-He213 Kinoshita, Mao verfasserin aut Kawaguchi, Ken verfasserin aut Kushimoto, Kohsuke verfasserin aut Yoshii, Ryogo verfasserin aut Inoue, Keita verfasserin aut Yamasaki, Masaki verfasserin aut Matsuyama, Tasuku verfasserin aut Kooguchi, Kunihiko verfasserin aut Takashima, Yasuo verfasserin aut Tanaka, Masami verfasserin aut Matsumoto, Kazumichi verfasserin aut Tashiro, Kei verfasserin aut Inaba, Tohru verfasserin aut Ohta, Bon verfasserin aut Sawa, Teiji verfasserin aut Enthalten in Critical care BioMed Central, 1997 28(2024), 1 vom: 23. Apr. (DE-627)331258269 (DE-600)2051256-9 1364-8535 nnns volume:28 year:2024 number:1 day:23 month:04 https://dx.doi.org/10.1186/s13054-024-04921-3 X:SPRINGER Resolving-System kostenfrei Volltext SYSFLAG_0 GBV_SPRINGER SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 44.00 VZ AR 28 2024 1 23 04 |
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10.1186/s13054-024-04921-3 doi (DE-627)SPR055620892 (SPR)s13054-024-04921-3-e DE-627 ger DE-627 rakwb eng 610 VZ 44.00 bkl Sudo, Kazuki verfasserin aut Case study observational research: inflammatory cytokines in the bronchial epithelial lining fluid of COVID-19 patients with acute hypoxemic respiratory failure 2024 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s) 2024 Background In this study, the concentrations of inflammatory cytokines were measured in the bronchial epithelial lining fluid (ELF) and plasma in patients with acute hypoxemic respiratory failure (AHRF) secondary to severe coronavirus disease 2019 (COVID-19). Methods We comprehensively analyzed the concentrations of 25 cytokines in the ELF and plasma of 27 COVID-19 AHRF patients. ELF was collected using the bronchial microsampling method through an endotracheal tube just after patients were intubated for mechanical ventilation. Results Compared with those in healthy volunteers, the concentrations of interleukin (IL)-6 (median 27.6 pmol/L), IL-8 (1045.1 pmol/L), IL-17A (0.8 pmol/L), IL-25 (1.5 pmol/L), and IL-31 (42.3 pmol/L) were significantly greater in the ELF of COVID-19 patients than in that of volunteers. The concentrations of MCP-1 and MIP-1β were significantly greater in the plasma of COVID-19 patients than in that of volunteers. The ELF/plasma ratio of IL-8 was the highest among the 25 cytokines, with a median of 737, and the ELF/plasma ratio of IL-6 (median: 218), IL-1β (202), IL-31 (169), MCP-1 (81), MIP-1β (55), and TNF-α (47) were lower. Conclusions The ELF concentrations of IL-6, IL-8, IL-17A, IL-25, and IL-31 were significantly increased in COVID-19 patients. Although high levels of MIP-1 and MIP-1β were also detected in the blood samples collected simultaneously with the ELF samples, the results indicated that lung inflammation was highly compartmentalized. Our study demonstrated that a comprehensive analysis of cytokines in the ELF is a feasible approach for understanding lung inflammation and systemic interactions in patients with severe pneumonia. Bronchial microsampling (dpeaa)DE-He213 COVID-19 (dpeaa)DE-He213 Cytokine (dpeaa)DE-He213 Epithelial lining fluid (dpeaa)DE-He213 Multiplex bead-based assay (dpeaa)DE-He213 Kinoshita, Mao verfasserin aut Kawaguchi, Ken verfasserin aut Kushimoto, Kohsuke verfasserin aut Yoshii, Ryogo verfasserin aut Inoue, Keita verfasserin aut Yamasaki, Masaki verfasserin aut Matsuyama, Tasuku verfasserin aut Kooguchi, Kunihiko verfasserin aut Takashima, Yasuo verfasserin aut Tanaka, Masami verfasserin aut Matsumoto, Kazumichi verfasserin aut Tashiro, Kei verfasserin aut Inaba, Tohru verfasserin aut Ohta, Bon verfasserin aut Sawa, Teiji verfasserin aut Enthalten in Critical care BioMed Central, 1997 28(2024), 1 vom: 23. Apr. (DE-627)331258269 (DE-600)2051256-9 1364-8535 nnns volume:28 year:2024 number:1 day:23 month:04 https://dx.doi.org/10.1186/s13054-024-04921-3 X:SPRINGER Resolving-System kostenfrei Volltext SYSFLAG_0 GBV_SPRINGER SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 44.00 VZ AR 28 2024 1 23 04 |
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10.1186/s13054-024-04921-3 doi (DE-627)SPR055620892 (SPR)s13054-024-04921-3-e DE-627 ger DE-627 rakwb eng 610 VZ 44.00 bkl Sudo, Kazuki verfasserin aut Case study observational research: inflammatory cytokines in the bronchial epithelial lining fluid of COVID-19 patients with acute hypoxemic respiratory failure 2024 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s) 2024 Background In this study, the concentrations of inflammatory cytokines were measured in the bronchial epithelial lining fluid (ELF) and plasma in patients with acute hypoxemic respiratory failure (AHRF) secondary to severe coronavirus disease 2019 (COVID-19). Methods We comprehensively analyzed the concentrations of 25 cytokines in the ELF and plasma of 27 COVID-19 AHRF patients. ELF was collected using the bronchial microsampling method through an endotracheal tube just after patients were intubated for mechanical ventilation. Results Compared with those in healthy volunteers, the concentrations of interleukin (IL)-6 (median 27.6 pmol/L), IL-8 (1045.1 pmol/L), IL-17A (0.8 pmol/L), IL-25 (1.5 pmol/L), and IL-31 (42.3 pmol/L) were significantly greater in the ELF of COVID-19 patients than in that of volunteers. The concentrations of MCP-1 and MIP-1β were significantly greater in the plasma of COVID-19 patients than in that of volunteers. The ELF/plasma ratio of IL-8 was the highest among the 25 cytokines, with a median of 737, and the ELF/plasma ratio of IL-6 (median: 218), IL-1β (202), IL-31 (169), MCP-1 (81), MIP-1β (55), and TNF-α (47) were lower. Conclusions The ELF concentrations of IL-6, IL-8, IL-17A, IL-25, and IL-31 were significantly increased in COVID-19 patients. Although high levels of MIP-1 and MIP-1β were also detected in the blood samples collected simultaneously with the ELF samples, the results indicated that lung inflammation was highly compartmentalized. Our study demonstrated that a comprehensive analysis of cytokines in the ELF is a feasible approach for understanding lung inflammation and systemic interactions in patients with severe pneumonia. Bronchial microsampling (dpeaa)DE-He213 COVID-19 (dpeaa)DE-He213 Cytokine (dpeaa)DE-He213 Epithelial lining fluid (dpeaa)DE-He213 Multiplex bead-based assay (dpeaa)DE-He213 Kinoshita, Mao verfasserin aut Kawaguchi, Ken verfasserin aut Kushimoto, Kohsuke verfasserin aut Yoshii, Ryogo verfasserin aut Inoue, Keita verfasserin aut Yamasaki, Masaki verfasserin aut Matsuyama, Tasuku verfasserin aut Kooguchi, Kunihiko verfasserin aut Takashima, Yasuo verfasserin aut Tanaka, Masami verfasserin aut Matsumoto, Kazumichi verfasserin aut Tashiro, Kei verfasserin aut Inaba, Tohru verfasserin aut Ohta, Bon verfasserin aut Sawa, Teiji verfasserin aut Enthalten in Critical care BioMed Central, 1997 28(2024), 1 vom: 23. Apr. (DE-627)331258269 (DE-600)2051256-9 1364-8535 nnns volume:28 year:2024 number:1 day:23 month:04 https://dx.doi.org/10.1186/s13054-024-04921-3 X:SPRINGER Resolving-System kostenfrei Volltext SYSFLAG_0 GBV_SPRINGER SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 44.00 VZ AR 28 2024 1 23 04 |
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Sudo, Kazuki Kinoshita, Mao Kawaguchi, Ken Kushimoto, Kohsuke Yoshii, Ryogo Inoue, Keita Yamasaki, Masaki Matsuyama, Tasuku Kooguchi, Kunihiko Takashima, Yasuo Tanaka, Masami Matsumoto, Kazumichi Tashiro, Kei Inaba, Tohru Ohta, Bon Sawa, Teiji |
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Elektronische Aufsätze |
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Sudo, Kazuki |
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case study observational research: inflammatory cytokines in the bronchial epithelial lining fluid of covid-19 patients with acute hypoxemic respiratory failure |
title_auth |
Case study observational research: inflammatory cytokines in the bronchial epithelial lining fluid of COVID-19 patients with acute hypoxemic respiratory failure |
abstract |
Background In this study, the concentrations of inflammatory cytokines were measured in the bronchial epithelial lining fluid (ELF) and plasma in patients with acute hypoxemic respiratory failure (AHRF) secondary to severe coronavirus disease 2019 (COVID-19). Methods We comprehensively analyzed the concentrations of 25 cytokines in the ELF and plasma of 27 COVID-19 AHRF patients. ELF was collected using the bronchial microsampling method through an endotracheal tube just after patients were intubated for mechanical ventilation. Results Compared with those in healthy volunteers, the concentrations of interleukin (IL)-6 (median 27.6 pmol/L), IL-8 (1045.1 pmol/L), IL-17A (0.8 pmol/L), IL-25 (1.5 pmol/L), and IL-31 (42.3 pmol/L) were significantly greater in the ELF of COVID-19 patients than in that of volunteers. The concentrations of MCP-1 and MIP-1β were significantly greater in the plasma of COVID-19 patients than in that of volunteers. The ELF/plasma ratio of IL-8 was the highest among the 25 cytokines, with a median of 737, and the ELF/plasma ratio of IL-6 (median: 218), IL-1β (202), IL-31 (169), MCP-1 (81), MIP-1β (55), and TNF-α (47) were lower. Conclusions The ELF concentrations of IL-6, IL-8, IL-17A, IL-25, and IL-31 were significantly increased in COVID-19 patients. Although high levels of MIP-1 and MIP-1β were also detected in the blood samples collected simultaneously with the ELF samples, the results indicated that lung inflammation was highly compartmentalized. Our study demonstrated that a comprehensive analysis of cytokines in the ELF is a feasible approach for understanding lung inflammation and systemic interactions in patients with severe pneumonia. © The Author(s) 2024 |
abstractGer |
Background In this study, the concentrations of inflammatory cytokines were measured in the bronchial epithelial lining fluid (ELF) and plasma in patients with acute hypoxemic respiratory failure (AHRF) secondary to severe coronavirus disease 2019 (COVID-19). Methods We comprehensively analyzed the concentrations of 25 cytokines in the ELF and plasma of 27 COVID-19 AHRF patients. ELF was collected using the bronchial microsampling method through an endotracheal tube just after patients were intubated for mechanical ventilation. Results Compared with those in healthy volunteers, the concentrations of interleukin (IL)-6 (median 27.6 pmol/L), IL-8 (1045.1 pmol/L), IL-17A (0.8 pmol/L), IL-25 (1.5 pmol/L), and IL-31 (42.3 pmol/L) were significantly greater in the ELF of COVID-19 patients than in that of volunteers. The concentrations of MCP-1 and MIP-1β were significantly greater in the plasma of COVID-19 patients than in that of volunteers. The ELF/plasma ratio of IL-8 was the highest among the 25 cytokines, with a median of 737, and the ELF/plasma ratio of IL-6 (median: 218), IL-1β (202), IL-31 (169), MCP-1 (81), MIP-1β (55), and TNF-α (47) were lower. Conclusions The ELF concentrations of IL-6, IL-8, IL-17A, IL-25, and IL-31 were significantly increased in COVID-19 patients. Although high levels of MIP-1 and MIP-1β were also detected in the blood samples collected simultaneously with the ELF samples, the results indicated that lung inflammation was highly compartmentalized. Our study demonstrated that a comprehensive analysis of cytokines in the ELF is a feasible approach for understanding lung inflammation and systemic interactions in patients with severe pneumonia. © The Author(s) 2024 |
abstract_unstemmed |
Background In this study, the concentrations of inflammatory cytokines were measured in the bronchial epithelial lining fluid (ELF) and plasma in patients with acute hypoxemic respiratory failure (AHRF) secondary to severe coronavirus disease 2019 (COVID-19). Methods We comprehensively analyzed the concentrations of 25 cytokines in the ELF and plasma of 27 COVID-19 AHRF patients. ELF was collected using the bronchial microsampling method through an endotracheal tube just after patients were intubated for mechanical ventilation. Results Compared with those in healthy volunteers, the concentrations of interleukin (IL)-6 (median 27.6 pmol/L), IL-8 (1045.1 pmol/L), IL-17A (0.8 pmol/L), IL-25 (1.5 pmol/L), and IL-31 (42.3 pmol/L) were significantly greater in the ELF of COVID-19 patients than in that of volunteers. The concentrations of MCP-1 and MIP-1β were significantly greater in the plasma of COVID-19 patients than in that of volunteers. The ELF/plasma ratio of IL-8 was the highest among the 25 cytokines, with a median of 737, and the ELF/plasma ratio of IL-6 (median: 218), IL-1β (202), IL-31 (169), MCP-1 (81), MIP-1β (55), and TNF-α (47) were lower. Conclusions The ELF concentrations of IL-6, IL-8, IL-17A, IL-25, and IL-31 were significantly increased in COVID-19 patients. Although high levels of MIP-1 and MIP-1β were also detected in the blood samples collected simultaneously with the ELF samples, the results indicated that lung inflammation was highly compartmentalized. Our study demonstrated that a comprehensive analysis of cytokines in the ELF is a feasible approach for understanding lung inflammation and systemic interactions in patients with severe pneumonia. © The Author(s) 2024 |
collection_details |
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Case study observational research: inflammatory cytokines in the bronchial epithelial lining fluid of COVID-19 patients with acute hypoxemic respiratory failure |
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https://dx.doi.org/10.1186/s13054-024-04921-3 |
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Kinoshita, Mao Kawaguchi, Ken Kushimoto, Kohsuke Yoshii, Ryogo Inoue, Keita Yamasaki, Masaki Matsuyama, Tasuku Kooguchi, Kunihiko Takashima, Yasuo Tanaka, Masami Matsumoto, Kazumichi Tashiro, Kei Inaba, Tohru Ohta, Bon Sawa, Teiji |
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Kinoshita, Mao Kawaguchi, Ken Kushimoto, Kohsuke Yoshii, Ryogo Inoue, Keita Yamasaki, Masaki Matsuyama, Tasuku Kooguchi, Kunihiko Takashima, Yasuo Tanaka, Masami Matsumoto, Kazumichi Tashiro, Kei Inaba, Tohru Ohta, Bon Sawa, Teiji |
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