Synthesis and characterization of silver nanoparticle conjugated-chalcones and their evaluation for antimalarial, cytotoxicity and haemolytic potential at in vitro level
Abstract Silver nanoparticles (AgNPs) have shown a wide range of antimicrobial activities over the last 2 decades, but little is known about their antimalarial activity. Therefore, in the present study, AgNPs were surface functionalized by chalcones to create an efficient bioactive molecular surface...
Ausführliche Beschreibung
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| Autor*in: |
Sinha, Shweta [verfasserIn] Kaur, Amandeep [verfasserIn] Sehgal, Rakesh [verfasserIn] |
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| Format: |
E-Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
2024 |
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| Schlagwörter: |
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| Anmerkung: |
© The Author(s) 2024 |
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| Übergeordnetes Werk: |
Enthalten in: Discover applied sciences - Springer International Publishing, 2024, 6(2024), 6 vom: 16. Mai |
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| Übergeordnetes Werk: |
volume:6 ; year:2024 ; number:6 ; day:16 ; month:05 |
| Links: |
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| DOI / URN: |
10.1007/s42452-024-05928-9 |
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SPR05589013X |
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| 520 | |a Abstract Silver nanoparticles (AgNPs) have shown a wide range of antimicrobial activities over the last 2 decades, but little is known about their antimalarial activity. Therefore, in the present study, AgNPs were surface functionalized by chalcones to create an efficient bioactive molecular surface that can enhance the antimalarial competency of both chalcones as well as chemically synthesized AgNPs. The AgNPs-conjugated chalcones have been synthesized using a chemical method employing the EDC-NHS coupling method. The characterization of AgNPs and AgNPs-conjugated chalcones was done through various analytical techniques. The SYBR Green I assay was performed for in vitro antimalarial activity, and cell cytotoxicity was done on HeLa cell line with MTT assay to calculate the $ IC_{50} $ and $ CC_{50} $, respectively. Haemolytic effect on fresh RBCs of these nanoconjugates were observed for 3 h and 24 h. AgNPs and AgNPs-conjugated chalcones have spectra at 420 nm and between 350 and 375 nm, respectively. The $ IC_{50} $ values of all the three conjugates for antimalarial activity ranged from 0.30 to 0.80 μg/mL. The present study provides a new method of synthesizing AgNPs-conjugated chalcones. Also, these synthesized conjugates show better antimalarial potential and reduced cellular toxicity compared to bared chalcones under an in vitro culture system. However, a further pre-clinical study on the murine model of malaria along with toxicity parameters is needed to provide more clarity. Graphical abstract | ||
| 520 | |a Article Highlights Chalcones have versatile pharmacological properties and can be conjugated with silver nanoparticles to enhance their antimalarial activity.The activity of these synthesized nanoconjugates was highly dependent on exposure time and concentration.The synthetic procedure can be performed with ease, is reproducible with similar chalcone scaffolds, and is inexpensive. | ||
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10.1007/s42452-024-05928-9 doi (DE-627)SPR05589013X (SPR)s42452-024-05928-9-e DE-627 ger DE-627 rakwb eng Sinha, Shweta verfasserin (orcid)0000-0003-4097-7011 aut Synthesis and characterization of silver nanoparticle conjugated-chalcones and their evaluation for antimalarial, cytotoxicity and haemolytic potential at in vitro level 2024 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s) 2024 Abstract Silver nanoparticles (AgNPs) have shown a wide range of antimicrobial activities over the last 2 decades, but little is known about their antimalarial activity. Therefore, in the present study, AgNPs were surface functionalized by chalcones to create an efficient bioactive molecular surface that can enhance the antimalarial competency of both chalcones as well as chemically synthesized AgNPs. The AgNPs-conjugated chalcones have been synthesized using a chemical method employing the EDC-NHS coupling method. The characterization of AgNPs and AgNPs-conjugated chalcones was done through various analytical techniques. The SYBR Green I assay was performed for in vitro antimalarial activity, and cell cytotoxicity was done on HeLa cell line with MTT assay to calculate the $ IC_{50} $ and $ CC_{50} $, respectively. Haemolytic effect on fresh RBCs of these nanoconjugates were observed for 3 h and 24 h. AgNPs and AgNPs-conjugated chalcones have spectra at 420 nm and between 350 and 375 nm, respectively. The $ IC_{50} $ values of all the three conjugates for antimalarial activity ranged from 0.30 to 0.80 μg/mL. The present study provides a new method of synthesizing AgNPs-conjugated chalcones. Also, these synthesized conjugates show better antimalarial potential and reduced cellular toxicity compared to bared chalcones under an in vitro culture system. However, a further pre-clinical study on the murine model of malaria along with toxicity parameters is needed to provide more clarity. Graphical abstract Article Highlights Chalcones have versatile pharmacological properties and can be conjugated with silver nanoparticles to enhance their antimalarial activity.The activity of these synthesized nanoconjugates was highly dependent on exposure time and concentration.The synthetic procedure can be performed with ease, is reproducible with similar chalcone scaffolds, and is inexpensive. Chalcones (dpeaa)DE-He213 Haemolysis (dpeaa)DE-He213 FTIR (dpeaa)DE-He213 Malaria (dpeaa)DE-He213 Silver nanoparticles (dpeaa)DE-He213 Kaur, Amandeep verfasserin aut Sehgal, Rakesh verfasserin (orcid)0000-0003-2910-4973 aut Enthalten in Discover applied sciences Springer International Publishing, 2024 6(2024), 6 vom: 16. Mai Online-Ressource (DE-627)1882945751 (DE-600)3181295-8 3004-9261 nnns volume:6 year:2024 number:6 day:16 month:05 https://dx.doi.org/10.1007/s42452-024-05928-9 X:SPRINGER Resolving-System kostenfrei Volltext SYSFLAG_0 GBV_SPRINGER GBV_ILN_20 GBV_ILN_22 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_171 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 6 2024 6 16 05 |
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10.1007/s42452-024-05928-9 doi (DE-627)SPR05589013X (SPR)s42452-024-05928-9-e DE-627 ger DE-627 rakwb eng Sinha, Shweta verfasserin (orcid)0000-0003-4097-7011 aut Synthesis and characterization of silver nanoparticle conjugated-chalcones and their evaluation for antimalarial, cytotoxicity and haemolytic potential at in vitro level 2024 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s) 2024 Abstract Silver nanoparticles (AgNPs) have shown a wide range of antimicrobial activities over the last 2 decades, but little is known about their antimalarial activity. Therefore, in the present study, AgNPs were surface functionalized by chalcones to create an efficient bioactive molecular surface that can enhance the antimalarial competency of both chalcones as well as chemically synthesized AgNPs. The AgNPs-conjugated chalcones have been synthesized using a chemical method employing the EDC-NHS coupling method. The characterization of AgNPs and AgNPs-conjugated chalcones was done through various analytical techniques. The SYBR Green I assay was performed for in vitro antimalarial activity, and cell cytotoxicity was done on HeLa cell line with MTT assay to calculate the $ IC_{50} $ and $ CC_{50} $, respectively. Haemolytic effect on fresh RBCs of these nanoconjugates were observed for 3 h and 24 h. AgNPs and AgNPs-conjugated chalcones have spectra at 420 nm and between 350 and 375 nm, respectively. The $ IC_{50} $ values of all the three conjugates for antimalarial activity ranged from 0.30 to 0.80 μg/mL. The present study provides a new method of synthesizing AgNPs-conjugated chalcones. Also, these synthesized conjugates show better antimalarial potential and reduced cellular toxicity compared to bared chalcones under an in vitro culture system. However, a further pre-clinical study on the murine model of malaria along with toxicity parameters is needed to provide more clarity. Graphical abstract Article Highlights Chalcones have versatile pharmacological properties and can be conjugated with silver nanoparticles to enhance their antimalarial activity.The activity of these synthesized nanoconjugates was highly dependent on exposure time and concentration.The synthetic procedure can be performed with ease, is reproducible with similar chalcone scaffolds, and is inexpensive. Chalcones (dpeaa)DE-He213 Haemolysis (dpeaa)DE-He213 FTIR (dpeaa)DE-He213 Malaria (dpeaa)DE-He213 Silver nanoparticles (dpeaa)DE-He213 Kaur, Amandeep verfasserin aut Sehgal, Rakesh verfasserin (orcid)0000-0003-2910-4973 aut Enthalten in Discover applied sciences Springer International Publishing, 2024 6(2024), 6 vom: 16. Mai Online-Ressource (DE-627)1882945751 (DE-600)3181295-8 3004-9261 nnns volume:6 year:2024 number:6 day:16 month:05 https://dx.doi.org/10.1007/s42452-024-05928-9 X:SPRINGER Resolving-System kostenfrei Volltext SYSFLAG_0 GBV_SPRINGER GBV_ILN_20 GBV_ILN_22 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_171 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 6 2024 6 16 05 |
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10.1007/s42452-024-05928-9 doi (DE-627)SPR05589013X (SPR)s42452-024-05928-9-e DE-627 ger DE-627 rakwb eng Sinha, Shweta verfasserin (orcid)0000-0003-4097-7011 aut Synthesis and characterization of silver nanoparticle conjugated-chalcones and their evaluation for antimalarial, cytotoxicity and haemolytic potential at in vitro level 2024 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s) 2024 Abstract Silver nanoparticles (AgNPs) have shown a wide range of antimicrobial activities over the last 2 decades, but little is known about their antimalarial activity. Therefore, in the present study, AgNPs were surface functionalized by chalcones to create an efficient bioactive molecular surface that can enhance the antimalarial competency of both chalcones as well as chemically synthesized AgNPs. The AgNPs-conjugated chalcones have been synthesized using a chemical method employing the EDC-NHS coupling method. The characterization of AgNPs and AgNPs-conjugated chalcones was done through various analytical techniques. The SYBR Green I assay was performed for in vitro antimalarial activity, and cell cytotoxicity was done on HeLa cell line with MTT assay to calculate the $ IC_{50} $ and $ CC_{50} $, respectively. Haemolytic effect on fresh RBCs of these nanoconjugates were observed for 3 h and 24 h. AgNPs and AgNPs-conjugated chalcones have spectra at 420 nm and between 350 and 375 nm, respectively. The $ IC_{50} $ values of all the three conjugates for antimalarial activity ranged from 0.30 to 0.80 μg/mL. The present study provides a new method of synthesizing AgNPs-conjugated chalcones. Also, these synthesized conjugates show better antimalarial potential and reduced cellular toxicity compared to bared chalcones under an in vitro culture system. However, a further pre-clinical study on the murine model of malaria along with toxicity parameters is needed to provide more clarity. Graphical abstract Article Highlights Chalcones have versatile pharmacological properties and can be conjugated with silver nanoparticles to enhance their antimalarial activity.The activity of these synthesized nanoconjugates was highly dependent on exposure time and concentration.The synthetic procedure can be performed with ease, is reproducible with similar chalcone scaffolds, and is inexpensive. Chalcones (dpeaa)DE-He213 Haemolysis (dpeaa)DE-He213 FTIR (dpeaa)DE-He213 Malaria (dpeaa)DE-He213 Silver nanoparticles (dpeaa)DE-He213 Kaur, Amandeep verfasserin aut Sehgal, Rakesh verfasserin (orcid)0000-0003-2910-4973 aut Enthalten in Discover applied sciences Springer International Publishing, 2024 6(2024), 6 vom: 16. Mai Online-Ressource (DE-627)1882945751 (DE-600)3181295-8 3004-9261 nnns volume:6 year:2024 number:6 day:16 month:05 https://dx.doi.org/10.1007/s42452-024-05928-9 X:SPRINGER Resolving-System kostenfrei Volltext SYSFLAG_0 GBV_SPRINGER GBV_ILN_20 GBV_ILN_22 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_171 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 6 2024 6 16 05 |
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10.1007/s42452-024-05928-9 doi (DE-627)SPR05589013X (SPR)s42452-024-05928-9-e DE-627 ger DE-627 rakwb eng Sinha, Shweta verfasserin (orcid)0000-0003-4097-7011 aut Synthesis and characterization of silver nanoparticle conjugated-chalcones and their evaluation for antimalarial, cytotoxicity and haemolytic potential at in vitro level 2024 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s) 2024 Abstract Silver nanoparticles (AgNPs) have shown a wide range of antimicrobial activities over the last 2 decades, but little is known about their antimalarial activity. Therefore, in the present study, AgNPs were surface functionalized by chalcones to create an efficient bioactive molecular surface that can enhance the antimalarial competency of both chalcones as well as chemically synthesized AgNPs. The AgNPs-conjugated chalcones have been synthesized using a chemical method employing the EDC-NHS coupling method. The characterization of AgNPs and AgNPs-conjugated chalcones was done through various analytical techniques. The SYBR Green I assay was performed for in vitro antimalarial activity, and cell cytotoxicity was done on HeLa cell line with MTT assay to calculate the $ IC_{50} $ and $ CC_{50} $, respectively. Haemolytic effect on fresh RBCs of these nanoconjugates were observed for 3 h and 24 h. AgNPs and AgNPs-conjugated chalcones have spectra at 420 nm and between 350 and 375 nm, respectively. The $ IC_{50} $ values of all the three conjugates for antimalarial activity ranged from 0.30 to 0.80 μg/mL. The present study provides a new method of synthesizing AgNPs-conjugated chalcones. Also, these synthesized conjugates show better antimalarial potential and reduced cellular toxicity compared to bared chalcones under an in vitro culture system. However, a further pre-clinical study on the murine model of malaria along with toxicity parameters is needed to provide more clarity. Graphical abstract Article Highlights Chalcones have versatile pharmacological properties and can be conjugated with silver nanoparticles to enhance their antimalarial activity.The activity of these synthesized nanoconjugates was highly dependent on exposure time and concentration.The synthetic procedure can be performed with ease, is reproducible with similar chalcone scaffolds, and is inexpensive. Chalcones (dpeaa)DE-He213 Haemolysis (dpeaa)DE-He213 FTIR (dpeaa)DE-He213 Malaria (dpeaa)DE-He213 Silver nanoparticles (dpeaa)DE-He213 Kaur, Amandeep verfasserin aut Sehgal, Rakesh verfasserin (orcid)0000-0003-2910-4973 aut Enthalten in Discover applied sciences Springer International Publishing, 2024 6(2024), 6 vom: 16. Mai Online-Ressource (DE-627)1882945751 (DE-600)3181295-8 3004-9261 nnns volume:6 year:2024 number:6 day:16 month:05 https://dx.doi.org/10.1007/s42452-024-05928-9 X:SPRINGER Resolving-System kostenfrei Volltext SYSFLAG_0 GBV_SPRINGER GBV_ILN_20 GBV_ILN_22 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_171 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 6 2024 6 16 05 |
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10.1007/s42452-024-05928-9 doi (DE-627)SPR05589013X (SPR)s42452-024-05928-9-e DE-627 ger DE-627 rakwb eng Sinha, Shweta verfasserin (orcid)0000-0003-4097-7011 aut Synthesis and characterization of silver nanoparticle conjugated-chalcones and their evaluation for antimalarial, cytotoxicity and haemolytic potential at in vitro level 2024 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s) 2024 Abstract Silver nanoparticles (AgNPs) have shown a wide range of antimicrobial activities over the last 2 decades, but little is known about their antimalarial activity. Therefore, in the present study, AgNPs were surface functionalized by chalcones to create an efficient bioactive molecular surface that can enhance the antimalarial competency of both chalcones as well as chemically synthesized AgNPs. The AgNPs-conjugated chalcones have been synthesized using a chemical method employing the EDC-NHS coupling method. The characterization of AgNPs and AgNPs-conjugated chalcones was done through various analytical techniques. The SYBR Green I assay was performed for in vitro antimalarial activity, and cell cytotoxicity was done on HeLa cell line with MTT assay to calculate the $ IC_{50} $ and $ CC_{50} $, respectively. Haemolytic effect on fresh RBCs of these nanoconjugates were observed for 3 h and 24 h. AgNPs and AgNPs-conjugated chalcones have spectra at 420 nm and between 350 and 375 nm, respectively. The $ IC_{50} $ values of all the three conjugates for antimalarial activity ranged from 0.30 to 0.80 μg/mL. The present study provides a new method of synthesizing AgNPs-conjugated chalcones. Also, these synthesized conjugates show better antimalarial potential and reduced cellular toxicity compared to bared chalcones under an in vitro culture system. However, a further pre-clinical study on the murine model of malaria along with toxicity parameters is needed to provide more clarity. Graphical abstract Article Highlights Chalcones have versatile pharmacological properties and can be conjugated with silver nanoparticles to enhance their antimalarial activity.The activity of these synthesized nanoconjugates was highly dependent on exposure time and concentration.The synthetic procedure can be performed with ease, is reproducible with similar chalcone scaffolds, and is inexpensive. Chalcones (dpeaa)DE-He213 Haemolysis (dpeaa)DE-He213 FTIR (dpeaa)DE-He213 Malaria (dpeaa)DE-He213 Silver nanoparticles (dpeaa)DE-He213 Kaur, Amandeep verfasserin aut Sehgal, Rakesh verfasserin (orcid)0000-0003-2910-4973 aut Enthalten in Discover applied sciences Springer International Publishing, 2024 6(2024), 6 vom: 16. Mai Online-Ressource (DE-627)1882945751 (DE-600)3181295-8 3004-9261 nnns volume:6 year:2024 number:6 day:16 month:05 https://dx.doi.org/10.1007/s42452-024-05928-9 X:SPRINGER Resolving-System kostenfrei Volltext SYSFLAG_0 GBV_SPRINGER GBV_ILN_20 GBV_ILN_22 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_171 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 6 2024 6 16 05 |
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Synthesis and characterization of silver nanoparticle conjugated-chalcones and their evaluation for antimalarial, cytotoxicity and haemolytic potential at in vitro level |
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Abstract Silver nanoparticles (AgNPs) have shown a wide range of antimicrobial activities over the last 2 decades, but little is known about their antimalarial activity. Therefore, in the present study, AgNPs were surface functionalized by chalcones to create an efficient bioactive molecular surface that can enhance the antimalarial competency of both chalcones as well as chemically synthesized AgNPs. The AgNPs-conjugated chalcones have been synthesized using a chemical method employing the EDC-NHS coupling method. The characterization of AgNPs and AgNPs-conjugated chalcones was done through various analytical techniques. The SYBR Green I assay was performed for in vitro antimalarial activity, and cell cytotoxicity was done on HeLa cell line with MTT assay to calculate the $ IC_{50} $ and $ CC_{50} $, respectively. Haemolytic effect on fresh RBCs of these nanoconjugates were observed for 3 h and 24 h. AgNPs and AgNPs-conjugated chalcones have spectra at 420 nm and between 350 and 375 nm, respectively. The $ IC_{50} $ values of all the three conjugates for antimalarial activity ranged from 0.30 to 0.80 μg/mL. The present study provides a new method of synthesizing AgNPs-conjugated chalcones. Also, these synthesized conjugates show better antimalarial potential and reduced cellular toxicity compared to bared chalcones under an in vitro culture system. However, a further pre-clinical study on the murine model of malaria along with toxicity parameters is needed to provide more clarity. Graphical abstract Article Highlights Chalcones have versatile pharmacological properties and can be conjugated with silver nanoparticles to enhance their antimalarial activity.The activity of these synthesized nanoconjugates was highly dependent on exposure time and concentration.The synthetic procedure can be performed with ease, is reproducible with similar chalcone scaffolds, and is inexpensive. © The Author(s) 2024 |
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Abstract Silver nanoparticles (AgNPs) have shown a wide range of antimicrobial activities over the last 2 decades, but little is known about their antimalarial activity. Therefore, in the present study, AgNPs were surface functionalized by chalcones to create an efficient bioactive molecular surface that can enhance the antimalarial competency of both chalcones as well as chemically synthesized AgNPs. The AgNPs-conjugated chalcones have been synthesized using a chemical method employing the EDC-NHS coupling method. The characterization of AgNPs and AgNPs-conjugated chalcones was done through various analytical techniques. The SYBR Green I assay was performed for in vitro antimalarial activity, and cell cytotoxicity was done on HeLa cell line with MTT assay to calculate the $ IC_{50} $ and $ CC_{50} $, respectively. Haemolytic effect on fresh RBCs of these nanoconjugates were observed for 3 h and 24 h. AgNPs and AgNPs-conjugated chalcones have spectra at 420 nm and between 350 and 375 nm, respectively. The $ IC_{50} $ values of all the three conjugates for antimalarial activity ranged from 0.30 to 0.80 μg/mL. The present study provides a new method of synthesizing AgNPs-conjugated chalcones. Also, these synthesized conjugates show better antimalarial potential and reduced cellular toxicity compared to bared chalcones under an in vitro culture system. However, a further pre-clinical study on the murine model of malaria along with toxicity parameters is needed to provide more clarity. Graphical abstract Article Highlights Chalcones have versatile pharmacological properties and can be conjugated with silver nanoparticles to enhance their antimalarial activity.The activity of these synthesized nanoconjugates was highly dependent on exposure time and concentration.The synthetic procedure can be performed with ease, is reproducible with similar chalcone scaffolds, and is inexpensive. © The Author(s) 2024 |
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Abstract Silver nanoparticles (AgNPs) have shown a wide range of antimicrobial activities over the last 2 decades, but little is known about their antimalarial activity. Therefore, in the present study, AgNPs were surface functionalized by chalcones to create an efficient bioactive molecular surface that can enhance the antimalarial competency of both chalcones as well as chemically synthesized AgNPs. The AgNPs-conjugated chalcones have been synthesized using a chemical method employing the EDC-NHS coupling method. The characterization of AgNPs and AgNPs-conjugated chalcones was done through various analytical techniques. The SYBR Green I assay was performed for in vitro antimalarial activity, and cell cytotoxicity was done on HeLa cell line with MTT assay to calculate the $ IC_{50} $ and $ CC_{50} $, respectively. Haemolytic effect on fresh RBCs of these nanoconjugates were observed for 3 h and 24 h. AgNPs and AgNPs-conjugated chalcones have spectra at 420 nm and between 350 and 375 nm, respectively. The $ IC_{50} $ values of all the three conjugates for antimalarial activity ranged from 0.30 to 0.80 μg/mL. The present study provides a new method of synthesizing AgNPs-conjugated chalcones. Also, these synthesized conjugates show better antimalarial potential and reduced cellular toxicity compared to bared chalcones under an in vitro culture system. However, a further pre-clinical study on the murine model of malaria along with toxicity parameters is needed to provide more clarity. Graphical abstract Article Highlights Chalcones have versatile pharmacological properties and can be conjugated with silver nanoparticles to enhance their antimalarial activity.The activity of these synthesized nanoconjugates was highly dependent on exposure time and concentration.The synthetic procedure can be performed with ease, is reproducible with similar chalcone scaffolds, and is inexpensive. © The Author(s) 2024 |
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The present study provides a new method of synthesizing AgNPs-conjugated chalcones. Also, these synthesized conjugates show better antimalarial potential and reduced cellular toxicity compared to bared chalcones under an in vitro culture system. However, a further pre-clinical study on the murine model of malaria along with toxicity parameters is needed to provide more clarity. Graphical abstract</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a">Article Highlights Chalcones have versatile pharmacological properties and can be conjugated with silver nanoparticles to enhance their antimalarial activity.The activity of these synthesized nanoconjugates was highly dependent on exposure time and concentration.The synthetic procedure can be performed with ease, is reproducible with similar chalcone scaffolds, and is inexpensive.</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Chalcones</subfield><subfield code="7">(dpeaa)DE-He213</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Haemolysis</subfield><subfield code="7">(dpeaa)DE-He213</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">FTIR</subfield><subfield code="7">(dpeaa)DE-He213</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Malaria</subfield><subfield code="7">(dpeaa)DE-He213</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Silver nanoparticles</subfield><subfield code="7">(dpeaa)DE-He213</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Kaur, Amandeep</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Sehgal, Rakesh</subfield><subfield code="e">verfasserin</subfield><subfield code="0">(orcid)0000-0003-2910-4973</subfield><subfield code="4">aut</subfield></datafield><datafield tag="773" ind1="0" ind2="8"><subfield code="i">Enthalten in</subfield><subfield code="t">Discover applied sciences</subfield><subfield code="d">Springer International Publishing, 2024</subfield><subfield code="g">6(2024), 6 vom: 16. Mai</subfield><subfield code="h">Online-Ressource</subfield><subfield code="w">(DE-627)1882945751</subfield><subfield code="w">(DE-600)3181295-8</subfield><subfield code="x">3004-9261</subfield><subfield code="7">nnns</subfield></datafield><datafield tag="773" ind1="1" ind2="8"><subfield code="g">volume:6</subfield><subfield code="g">year:2024</subfield><subfield code="g">number:6</subfield><subfield code="g">day:16</subfield><subfield code="g">month:05</subfield></datafield><datafield tag="856" ind1="4" ind2="0"><subfield code="u">https://dx.doi.org/10.1007/s42452-024-05928-9</subfield><subfield code="m">X:SPRINGER</subfield><subfield code="x">Resolving-System</subfield><subfield code="z">kostenfrei</subfield><subfield code="3">Volltext</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">SYSFLAG_0</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_SPRINGER</subfield></datafield><datafield tag="912" ind1=" " ind2=" 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