Development of microsatellite markers for the soft tick Ornithodoros phacochoerus
Background Soft ticks of the genus Ornithodoros are responsible for the maintenance and transmission of the African swine fever (ASF) virus in the sylvatic and domestic viral cycles in Southern Africa. They are also the main vectors of the Borrelia species causing relapsing fevers. Currently, no gen...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
Taraveau, Florian [verfasserIn] Bru, David [verfasserIn] Quembo, Carlos João [verfasserIn] Jourdan-Pineau, Hélène [verfasserIn] |
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| Format: |
E-Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
2024 |
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| Schlagwörter: |
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| Anmerkung: |
© The Author(s) 2024 |
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| Übergeordnetes Werk: |
Enthalten in: Parasites & vectors - BioMed Central, 2008, 17(2024), 1 vom: 11. Juli |
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| Übergeordnetes Werk: |
volume:17 ; year:2024 ; number:1 ; day:11 ; month:07 |
| Links: |
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| DOI / URN: |
10.1186/s13071-024-06382-7 |
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| Katalog-ID: |
SPR056554974 |
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| 520 | |a Background Soft ticks of the genus Ornithodoros are responsible for the maintenance and transmission of the African swine fever (ASF) virus in the sylvatic and domestic viral cycles in Southern Africa. They are also the main vectors of the Borrelia species causing relapsing fevers. Currently, no genetic markers are available for Afrotropical Ornithodoros ticks. As ASF spreads globally, such markers are needed to assess the role of ticks in the emergence of new outbreaks. The aim of this study is to design microsatellite markers that could be used for ticks of the Ornithodoros moubata complex, particularly Ornithodoros phacochoerus, to assess population structure and tick movements in ASF endemic areas. Methods A total of 151 markers were designed using the O. moubata and O. porcinus genomes after elimination of repeated sequences in the genomes. All designed markers were tested on O. phacochoerus and O. porcinus DNA to select the best markers. Results A total of 24 microsatellite markers were genotyped on two populations of O. phacochoerus and on individuals from four other Ornithodoros species. Nineteen markers were selected to be as robust as possible for population genetic studies on O. phacochoerus. Conclusions The microsatellite markers developed here represent the first genetic tool to study nidicolous populations of O. phacochoerus. Graphical Abstract | ||
| 650 | 4 | |a Vector surveillance |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a Soft tick |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a Population genetics |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a Microsatellite markers |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a African swine fever |7 (dpeaa)DE-He213 | |
| 700 | 1 | |a Bru, David |e verfasserin |4 aut | |
| 700 | 1 | |a Quembo, Carlos João |e verfasserin |4 aut | |
| 700 | 1 | |a Jourdan-Pineau, Hélène |e verfasserin |4 aut | |
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10.1186/s13071-024-06382-7 doi (DE-627)SPR056554974 (SPR)s13071-024-06382-7-e DE-627 ger DE-627 rakwb eng 570 VZ BIODIV DE-30 fid Taraveau, Florian verfasserin aut Development of microsatellite markers for the soft tick Ornithodoros phacochoerus 2024 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s) 2024 Background Soft ticks of the genus Ornithodoros are responsible for the maintenance and transmission of the African swine fever (ASF) virus in the sylvatic and domestic viral cycles in Southern Africa. They are also the main vectors of the Borrelia species causing relapsing fevers. Currently, no genetic markers are available for Afrotropical Ornithodoros ticks. As ASF spreads globally, such markers are needed to assess the role of ticks in the emergence of new outbreaks. The aim of this study is to design microsatellite markers that could be used for ticks of the Ornithodoros moubata complex, particularly Ornithodoros phacochoerus, to assess population structure and tick movements in ASF endemic areas. Methods A total of 151 markers were designed using the O. moubata and O. porcinus genomes after elimination of repeated sequences in the genomes. All designed markers were tested on O. phacochoerus and O. porcinus DNA to select the best markers. Results A total of 24 microsatellite markers were genotyped on two populations of O. phacochoerus and on individuals from four other Ornithodoros species. Nineteen markers were selected to be as robust as possible for population genetic studies on O. phacochoerus. Conclusions The microsatellite markers developed here represent the first genetic tool to study nidicolous populations of O. phacochoerus. Graphical Abstract Vector surveillance (dpeaa)DE-He213 Soft tick (dpeaa)DE-He213 Population genetics (dpeaa)DE-He213 Microsatellite markers (dpeaa)DE-He213 African swine fever (dpeaa)DE-He213 Bru, David verfasserin aut Quembo, Carlos João verfasserin aut Jourdan-Pineau, Hélène verfasserin aut Enthalten in Parasites & vectors BioMed Central, 2008 17(2024), 1 vom: 11. Juli (DE-627)558690076 (DE-600)2409480-8 1756-3305 nnns volume:17 year:2024 number:1 day:11 month:07 https://dx.doi.org/10.1186/s13071-024-06382-7 X:SPRINGER Resolving-System kostenfrei Volltext SYSFLAG_0 GBV_SPRINGER FID-BIODIV SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 17 2024 1 11 07 |
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10.1186/s13071-024-06382-7 doi (DE-627)SPR056554974 (SPR)s13071-024-06382-7-e DE-627 ger DE-627 rakwb eng 570 VZ BIODIV DE-30 fid Taraveau, Florian verfasserin aut Development of microsatellite markers for the soft tick Ornithodoros phacochoerus 2024 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s) 2024 Background Soft ticks of the genus Ornithodoros are responsible for the maintenance and transmission of the African swine fever (ASF) virus in the sylvatic and domestic viral cycles in Southern Africa. They are also the main vectors of the Borrelia species causing relapsing fevers. Currently, no genetic markers are available for Afrotropical Ornithodoros ticks. As ASF spreads globally, such markers are needed to assess the role of ticks in the emergence of new outbreaks. The aim of this study is to design microsatellite markers that could be used for ticks of the Ornithodoros moubata complex, particularly Ornithodoros phacochoerus, to assess population structure and tick movements in ASF endemic areas. Methods A total of 151 markers were designed using the O. moubata and O. porcinus genomes after elimination of repeated sequences in the genomes. All designed markers were tested on O. phacochoerus and O. porcinus DNA to select the best markers. Results A total of 24 microsatellite markers were genotyped on two populations of O. phacochoerus and on individuals from four other Ornithodoros species. Nineteen markers were selected to be as robust as possible for population genetic studies on O. phacochoerus. Conclusions The microsatellite markers developed here represent the first genetic tool to study nidicolous populations of O. phacochoerus. Graphical Abstract Vector surveillance (dpeaa)DE-He213 Soft tick (dpeaa)DE-He213 Population genetics (dpeaa)DE-He213 Microsatellite markers (dpeaa)DE-He213 African swine fever (dpeaa)DE-He213 Bru, David verfasserin aut Quembo, Carlos João verfasserin aut Jourdan-Pineau, Hélène verfasserin aut Enthalten in Parasites & vectors BioMed Central, 2008 17(2024), 1 vom: 11. Juli (DE-627)558690076 (DE-600)2409480-8 1756-3305 nnns volume:17 year:2024 number:1 day:11 month:07 https://dx.doi.org/10.1186/s13071-024-06382-7 X:SPRINGER Resolving-System kostenfrei Volltext SYSFLAG_0 GBV_SPRINGER FID-BIODIV SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 17 2024 1 11 07 |
| allfields_unstemmed |
10.1186/s13071-024-06382-7 doi (DE-627)SPR056554974 (SPR)s13071-024-06382-7-e DE-627 ger DE-627 rakwb eng 570 VZ BIODIV DE-30 fid Taraveau, Florian verfasserin aut Development of microsatellite markers for the soft tick Ornithodoros phacochoerus 2024 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s) 2024 Background Soft ticks of the genus Ornithodoros are responsible for the maintenance and transmission of the African swine fever (ASF) virus in the sylvatic and domestic viral cycles in Southern Africa. They are also the main vectors of the Borrelia species causing relapsing fevers. Currently, no genetic markers are available for Afrotropical Ornithodoros ticks. As ASF spreads globally, such markers are needed to assess the role of ticks in the emergence of new outbreaks. The aim of this study is to design microsatellite markers that could be used for ticks of the Ornithodoros moubata complex, particularly Ornithodoros phacochoerus, to assess population structure and tick movements in ASF endemic areas. Methods A total of 151 markers were designed using the O. moubata and O. porcinus genomes after elimination of repeated sequences in the genomes. All designed markers were tested on O. phacochoerus and O. porcinus DNA to select the best markers. Results A total of 24 microsatellite markers were genotyped on two populations of O. phacochoerus and on individuals from four other Ornithodoros species. Nineteen markers were selected to be as robust as possible for population genetic studies on O. phacochoerus. Conclusions The microsatellite markers developed here represent the first genetic tool to study nidicolous populations of O. phacochoerus. Graphical Abstract Vector surveillance (dpeaa)DE-He213 Soft tick (dpeaa)DE-He213 Population genetics (dpeaa)DE-He213 Microsatellite markers (dpeaa)DE-He213 African swine fever (dpeaa)DE-He213 Bru, David verfasserin aut Quembo, Carlos João verfasserin aut Jourdan-Pineau, Hélène verfasserin aut Enthalten in Parasites & vectors BioMed Central, 2008 17(2024), 1 vom: 11. Juli (DE-627)558690076 (DE-600)2409480-8 1756-3305 nnns volume:17 year:2024 number:1 day:11 month:07 https://dx.doi.org/10.1186/s13071-024-06382-7 X:SPRINGER Resolving-System kostenfrei Volltext SYSFLAG_0 GBV_SPRINGER FID-BIODIV SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 17 2024 1 11 07 |
| allfieldsGer |
10.1186/s13071-024-06382-7 doi (DE-627)SPR056554974 (SPR)s13071-024-06382-7-e DE-627 ger DE-627 rakwb eng 570 VZ BIODIV DE-30 fid Taraveau, Florian verfasserin aut Development of microsatellite markers for the soft tick Ornithodoros phacochoerus 2024 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s) 2024 Background Soft ticks of the genus Ornithodoros are responsible for the maintenance and transmission of the African swine fever (ASF) virus in the sylvatic and domestic viral cycles in Southern Africa. They are also the main vectors of the Borrelia species causing relapsing fevers. Currently, no genetic markers are available for Afrotropical Ornithodoros ticks. As ASF spreads globally, such markers are needed to assess the role of ticks in the emergence of new outbreaks. The aim of this study is to design microsatellite markers that could be used for ticks of the Ornithodoros moubata complex, particularly Ornithodoros phacochoerus, to assess population structure and tick movements in ASF endemic areas. Methods A total of 151 markers were designed using the O. moubata and O. porcinus genomes after elimination of repeated sequences in the genomes. All designed markers were tested on O. phacochoerus and O. porcinus DNA to select the best markers. Results A total of 24 microsatellite markers were genotyped on two populations of O. phacochoerus and on individuals from four other Ornithodoros species. Nineteen markers were selected to be as robust as possible for population genetic studies on O. phacochoerus. Conclusions The microsatellite markers developed here represent the first genetic tool to study nidicolous populations of O. phacochoerus. Graphical Abstract Vector surveillance (dpeaa)DE-He213 Soft tick (dpeaa)DE-He213 Population genetics (dpeaa)DE-He213 Microsatellite markers (dpeaa)DE-He213 African swine fever (dpeaa)DE-He213 Bru, David verfasserin aut Quembo, Carlos João verfasserin aut Jourdan-Pineau, Hélène verfasserin aut Enthalten in Parasites & vectors BioMed Central, 2008 17(2024), 1 vom: 11. Juli (DE-627)558690076 (DE-600)2409480-8 1756-3305 nnns volume:17 year:2024 number:1 day:11 month:07 https://dx.doi.org/10.1186/s13071-024-06382-7 X:SPRINGER Resolving-System kostenfrei Volltext SYSFLAG_0 GBV_SPRINGER FID-BIODIV SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 17 2024 1 11 07 |
| allfieldsSound |
10.1186/s13071-024-06382-7 doi (DE-627)SPR056554974 (SPR)s13071-024-06382-7-e DE-627 ger DE-627 rakwb eng 570 VZ BIODIV DE-30 fid Taraveau, Florian verfasserin aut Development of microsatellite markers for the soft tick Ornithodoros phacochoerus 2024 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s) 2024 Background Soft ticks of the genus Ornithodoros are responsible for the maintenance and transmission of the African swine fever (ASF) virus in the sylvatic and domestic viral cycles in Southern Africa. They are also the main vectors of the Borrelia species causing relapsing fevers. Currently, no genetic markers are available for Afrotropical Ornithodoros ticks. As ASF spreads globally, such markers are needed to assess the role of ticks in the emergence of new outbreaks. The aim of this study is to design microsatellite markers that could be used for ticks of the Ornithodoros moubata complex, particularly Ornithodoros phacochoerus, to assess population structure and tick movements in ASF endemic areas. Methods A total of 151 markers were designed using the O. moubata and O. porcinus genomes after elimination of repeated sequences in the genomes. All designed markers were tested on O. phacochoerus and O. porcinus DNA to select the best markers. Results A total of 24 microsatellite markers were genotyped on two populations of O. phacochoerus and on individuals from four other Ornithodoros species. Nineteen markers were selected to be as robust as possible for population genetic studies on O. phacochoerus. Conclusions The microsatellite markers developed here represent the first genetic tool to study nidicolous populations of O. phacochoerus. Graphical Abstract Vector surveillance (dpeaa)DE-He213 Soft tick (dpeaa)DE-He213 Population genetics (dpeaa)DE-He213 Microsatellite markers (dpeaa)DE-He213 African swine fever (dpeaa)DE-He213 Bru, David verfasserin aut Quembo, Carlos João verfasserin aut Jourdan-Pineau, Hélène verfasserin aut Enthalten in Parasites & vectors BioMed Central, 2008 17(2024), 1 vom: 11. Juli (DE-627)558690076 (DE-600)2409480-8 1756-3305 nnns volume:17 year:2024 number:1 day:11 month:07 https://dx.doi.org/10.1186/s13071-024-06382-7 X:SPRINGER Resolving-System kostenfrei Volltext SYSFLAG_0 GBV_SPRINGER FID-BIODIV SSG-OLC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 17 2024 1 11 07 |
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development of microsatellite markers for the soft tick ornithodoros phacochoerus |
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Development of microsatellite markers for the soft tick Ornithodoros phacochoerus |
| abstract |
Background Soft ticks of the genus Ornithodoros are responsible for the maintenance and transmission of the African swine fever (ASF) virus in the sylvatic and domestic viral cycles in Southern Africa. They are also the main vectors of the Borrelia species causing relapsing fevers. Currently, no genetic markers are available for Afrotropical Ornithodoros ticks. As ASF spreads globally, such markers are needed to assess the role of ticks in the emergence of new outbreaks. The aim of this study is to design microsatellite markers that could be used for ticks of the Ornithodoros moubata complex, particularly Ornithodoros phacochoerus, to assess population structure and tick movements in ASF endemic areas. Methods A total of 151 markers were designed using the O. moubata and O. porcinus genomes after elimination of repeated sequences in the genomes. All designed markers were tested on O. phacochoerus and O. porcinus DNA to select the best markers. Results A total of 24 microsatellite markers were genotyped on two populations of O. phacochoerus and on individuals from four other Ornithodoros species. Nineteen markers were selected to be as robust as possible for population genetic studies on O. phacochoerus. Conclusions The microsatellite markers developed here represent the first genetic tool to study nidicolous populations of O. phacochoerus. Graphical Abstract © The Author(s) 2024 |
| abstractGer |
Background Soft ticks of the genus Ornithodoros are responsible for the maintenance and transmission of the African swine fever (ASF) virus in the sylvatic and domestic viral cycles in Southern Africa. They are also the main vectors of the Borrelia species causing relapsing fevers. Currently, no genetic markers are available for Afrotropical Ornithodoros ticks. As ASF spreads globally, such markers are needed to assess the role of ticks in the emergence of new outbreaks. The aim of this study is to design microsatellite markers that could be used for ticks of the Ornithodoros moubata complex, particularly Ornithodoros phacochoerus, to assess population structure and tick movements in ASF endemic areas. Methods A total of 151 markers were designed using the O. moubata and O. porcinus genomes after elimination of repeated sequences in the genomes. All designed markers were tested on O. phacochoerus and O. porcinus DNA to select the best markers. Results A total of 24 microsatellite markers were genotyped on two populations of O. phacochoerus and on individuals from four other Ornithodoros species. Nineteen markers were selected to be as robust as possible for population genetic studies on O. phacochoerus. Conclusions The microsatellite markers developed here represent the first genetic tool to study nidicolous populations of O. phacochoerus. Graphical Abstract © The Author(s) 2024 |
| abstract_unstemmed |
Background Soft ticks of the genus Ornithodoros are responsible for the maintenance and transmission of the African swine fever (ASF) virus in the sylvatic and domestic viral cycles in Southern Africa. They are also the main vectors of the Borrelia species causing relapsing fevers. Currently, no genetic markers are available for Afrotropical Ornithodoros ticks. As ASF spreads globally, such markers are needed to assess the role of ticks in the emergence of new outbreaks. The aim of this study is to design microsatellite markers that could be used for ticks of the Ornithodoros moubata complex, particularly Ornithodoros phacochoerus, to assess population structure and tick movements in ASF endemic areas. Methods A total of 151 markers were designed using the O. moubata and O. porcinus genomes after elimination of repeated sequences in the genomes. All designed markers were tested on O. phacochoerus and O. porcinus DNA to select the best markers. Results A total of 24 microsatellite markers were genotyped on two populations of O. phacochoerus and on individuals from four other Ornithodoros species. Nineteen markers were selected to be as robust as possible for population genetic studies on O. phacochoerus. Conclusions The microsatellite markers developed here represent the first genetic tool to study nidicolous populations of O. phacochoerus. Graphical Abstract © The Author(s) 2024 |
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They are also the main vectors of the Borrelia species causing relapsing fevers. Currently, no genetic markers are available for Afrotropical Ornithodoros ticks. As ASF spreads globally, such markers are needed to assess the role of ticks in the emergence of new outbreaks. The aim of this study is to design microsatellite markers that could be used for ticks of the Ornithodoros moubata complex, particularly Ornithodoros phacochoerus, to assess population structure and tick movements in ASF endemic areas. Methods A total of 151 markers were designed using the O. moubata and O. porcinus genomes after elimination of repeated sequences in the genomes. All designed markers were tested on O. phacochoerus and O. porcinus DNA to select the best markers. Results A total of 24 microsatellite markers were genotyped on two populations of O. phacochoerus and on individuals from four other Ornithodoros species. Nineteen markers were selected to be as robust as possible for population genetic studies on O. phacochoerus. Conclusions The microsatellite markers developed here represent the first genetic tool to study nidicolous populations of O. phacochoerus. 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