Cryopreservation of human kidney organoids

Abstract Recent advances in stem cell research have led to the creation of organoids, miniature replicas of human organs, offering innovative avenues for studying diseases. Kidney organoids, with their ability to replicate complex renal structures, provide a novel platform for investigating kidney d...
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Gespeichert in:

Autor*in:	Mashouf, Parham [verfasserIn] Tabibzadeh, Nahid [verfasserIn] Kuraoka, Shohei [verfasserIn] Oishi, Haruka [verfasserIn] Morizane, Ryuji [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2024

Schlagwörter:	Vitrification Freezing Kidney Organoids Cryopreservation Dimethyl sulfoxide

Anmerkung:	© The Author(s) 2024

Übergeordnetes Werk:	Enthalten in: Cellular and molecular life sciences - Springer International Publishing, 1997, 81(2024), 1 vom: 18. Juli
Übergeordnetes Werk:	volume:81 ; year:2024 ; number:1 ; day:18 ; month:07

Links:	Volltext

DOI / URN:	10.1007/s00018-024-05352-7

Katalog-ID:	SPR056634498

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allfields	10.1007/s00018-024-05352-7 doi (DE-627)SPR056634498 (SPR)s00018-024-05352-7-e DE-627 ger DE-627 rakwb eng 570 610 VZ 42.15 bkl Mashouf, Parham verfasserin aut Cryopreservation of human kidney organoids 2024 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s) 2024 Abstract Recent advances in stem cell research have led to the creation of organoids, miniature replicas of human organs, offering innovative avenues for studying diseases. Kidney organoids, with their ability to replicate complex renal structures, provide a novel platform for investigating kidney diseases and assessing drug efficacy, albeit hindered by labor-intensive generation and batch variations, highlighting the need for tailored cryopreservation methods to enable widespread utilization. Here, we evaluated cryopreservation strategies for kidney organoids by contrasting slow-freezing and vitrification methods. 118 kidney organoids were categorized into five conditions. Control organoids followed standard culture, while two slow-freezing groups used 10% DMSO (SF1) or commercial freezing media (SF2). Vitrification involved V1 (20% DMSO, 20% Ethylene Glycol with sucrose) and V2 (15% DMSO, 15% Ethylene Glycol). Assessment of viability, functionality, and structural integrity post-thawing revealed notable differences. Vitrification, particularly V1, exhibited superior viability (91% for V1, 26% for V2, 79% for SF1, and 83% for SF2 compared to 99.4% in controls). 3D imaging highlighted distinct nephron segments among groups, emphasizing V1’s efficacy in preserving both podocytes and tubules in kidney organoids. Cisplatin-induced injury revealed a significant reduction in regenerative capacities in organoids cryopreserved by flow-freezing methods, while the V1 method did not show statistical significance compared to the unfrozen controls. This study underscores vitrification, especially with high concentrations of cryoprotectants, as an effective approach for maintaining kidney organoid viability and structure during cryopreservation, offering practical approaches for kidney organoid research. Vitrification (dpeaa)DE-He213 Freezing (dpeaa)DE-He213 Kidney (dpeaa)DE-He213 Organoids (dpeaa)DE-He213 Cryopreservation (dpeaa)DE-He213 Dimethyl sulfoxide (dpeaa)DE-He213 Tabibzadeh, Nahid verfasserin aut Kuraoka, Shohei verfasserin aut Oishi, Haruka verfasserin aut Morizane, Ryuji verfasserin (orcid)0000-0002-0377-5667 aut Enthalten in Cellular and molecular life sciences Springer International Publishing, 1997 81(2024), 1 vom: 18. Juli (DE-627)253390524 (DE-600)1458497-9 1420-9071 nnns volume:81 year:2024 number:1 day:18 month:07 https://dx.doi.org/10.1007/s00018-024-05352-7 X:SPRINGER Resolving-System kostenfrei Volltext SYSFLAG_0 GBV_SPRINGER SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_165 GBV_ILN_170 GBV_ILN_187 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_267 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2005 GBV_ILN_2014 GBV_ILN_2021 GBV_ILN_2050 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 42.15 VZ AR 81 2024 1 18 07
spelling	10.1007/s00018-024-05352-7 doi (DE-627)SPR056634498 (SPR)s00018-024-05352-7-e DE-627 ger DE-627 rakwb eng 570 610 VZ 42.15 bkl Mashouf, Parham verfasserin aut Cryopreservation of human kidney organoids 2024 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s) 2024 Abstract Recent advances in stem cell research have led to the creation of organoids, miniature replicas of human organs, offering innovative avenues for studying diseases. Kidney organoids, with their ability to replicate complex renal structures, provide a novel platform for investigating kidney diseases and assessing drug efficacy, albeit hindered by labor-intensive generation and batch variations, highlighting the need for tailored cryopreservation methods to enable widespread utilization. Here, we evaluated cryopreservation strategies for kidney organoids by contrasting slow-freezing and vitrification methods. 118 kidney organoids were categorized into five conditions. Control organoids followed standard culture, while two slow-freezing groups used 10% DMSO (SF1) or commercial freezing media (SF2). Vitrification involved V1 (20% DMSO, 20% Ethylene Glycol with sucrose) and V2 (15% DMSO, 15% Ethylene Glycol). Assessment of viability, functionality, and structural integrity post-thawing revealed notable differences. Vitrification, particularly V1, exhibited superior viability (91% for V1, 26% for V2, 79% for SF1, and 83% for SF2 compared to 99.4% in controls). 3D imaging highlighted distinct nephron segments among groups, emphasizing V1’s efficacy in preserving both podocytes and tubules in kidney organoids. Cisplatin-induced injury revealed a significant reduction in regenerative capacities in organoids cryopreserved by flow-freezing methods, while the V1 method did not show statistical significance compared to the unfrozen controls. This study underscores vitrification, especially with high concentrations of cryoprotectants, as an effective approach for maintaining kidney organoid viability and structure during cryopreservation, offering practical approaches for kidney organoid research. Vitrification (dpeaa)DE-He213 Freezing (dpeaa)DE-He213 Kidney (dpeaa)DE-He213 Organoids (dpeaa)DE-He213 Cryopreservation (dpeaa)DE-He213 Dimethyl sulfoxide (dpeaa)DE-He213 Tabibzadeh, Nahid verfasserin aut Kuraoka, Shohei verfasserin aut Oishi, Haruka verfasserin aut Morizane, Ryuji verfasserin (orcid)0000-0002-0377-5667 aut Enthalten in Cellular and molecular life sciences Springer International Publishing, 1997 81(2024), 1 vom: 18. Juli (DE-627)253390524 (DE-600)1458497-9 1420-9071 nnns volume:81 year:2024 number:1 day:18 month:07 https://dx.doi.org/10.1007/s00018-024-05352-7 X:SPRINGER Resolving-System kostenfrei Volltext SYSFLAG_0 GBV_SPRINGER SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_165 GBV_ILN_170 GBV_ILN_187 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_267 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2005 GBV_ILN_2014 GBV_ILN_2021 GBV_ILN_2050 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 42.15 VZ AR 81 2024 1 18 07
allfields_unstemmed	10.1007/s00018-024-05352-7 doi (DE-627)SPR056634498 (SPR)s00018-024-05352-7-e DE-627 ger DE-627 rakwb eng 570 610 VZ 42.15 bkl Mashouf, Parham verfasserin aut Cryopreservation of human kidney organoids 2024 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s) 2024 Abstract Recent advances in stem cell research have led to the creation of organoids, miniature replicas of human organs, offering innovative avenues for studying diseases. Kidney organoids, with their ability to replicate complex renal structures, provide a novel platform for investigating kidney diseases and assessing drug efficacy, albeit hindered by labor-intensive generation and batch variations, highlighting the need for tailored cryopreservation methods to enable widespread utilization. Here, we evaluated cryopreservation strategies for kidney organoids by contrasting slow-freezing and vitrification methods. 118 kidney organoids were categorized into five conditions. Control organoids followed standard culture, while two slow-freezing groups used 10% DMSO (SF1) or commercial freezing media (SF2). Vitrification involved V1 (20% DMSO, 20% Ethylene Glycol with sucrose) and V2 (15% DMSO, 15% Ethylene Glycol). Assessment of viability, functionality, and structural integrity post-thawing revealed notable differences. Vitrification, particularly V1, exhibited superior viability (91% for V1, 26% for V2, 79% for SF1, and 83% for SF2 compared to 99.4% in controls). 3D imaging highlighted distinct nephron segments among groups, emphasizing V1’s efficacy in preserving both podocytes and tubules in kidney organoids. Cisplatin-induced injury revealed a significant reduction in regenerative capacities in organoids cryopreserved by flow-freezing methods, while the V1 method did not show statistical significance compared to the unfrozen controls. This study underscores vitrification, especially with high concentrations of cryoprotectants, as an effective approach for maintaining kidney organoid viability and structure during cryopreservation, offering practical approaches for kidney organoid research. Vitrification (dpeaa)DE-He213 Freezing (dpeaa)DE-He213 Kidney (dpeaa)DE-He213 Organoids (dpeaa)DE-He213 Cryopreservation (dpeaa)DE-He213 Dimethyl sulfoxide (dpeaa)DE-He213 Tabibzadeh, Nahid verfasserin aut Kuraoka, Shohei verfasserin aut Oishi, Haruka verfasserin aut Morizane, Ryuji verfasserin (orcid)0000-0002-0377-5667 aut Enthalten in Cellular and molecular life sciences Springer International Publishing, 1997 81(2024), 1 vom: 18. Juli (DE-627)253390524 (DE-600)1458497-9 1420-9071 nnns volume:81 year:2024 number:1 day:18 month:07 https://dx.doi.org/10.1007/s00018-024-05352-7 X:SPRINGER Resolving-System kostenfrei Volltext SYSFLAG_0 GBV_SPRINGER SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_165 GBV_ILN_170 GBV_ILN_187 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_267 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2005 GBV_ILN_2014 GBV_ILN_2021 GBV_ILN_2050 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 42.15 VZ AR 81 2024 1 18 07
allfieldsGer	10.1007/s00018-024-05352-7 doi (DE-627)SPR056634498 (SPR)s00018-024-05352-7-e DE-627 ger DE-627 rakwb eng 570 610 VZ 42.15 bkl Mashouf, Parham verfasserin aut Cryopreservation of human kidney organoids 2024 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s) 2024 Abstract Recent advances in stem cell research have led to the creation of organoids, miniature replicas of human organs, offering innovative avenues for studying diseases. Kidney organoids, with their ability to replicate complex renal structures, provide a novel platform for investigating kidney diseases and assessing drug efficacy, albeit hindered by labor-intensive generation and batch variations, highlighting the need for tailored cryopreservation methods to enable widespread utilization. Here, we evaluated cryopreservation strategies for kidney organoids by contrasting slow-freezing and vitrification methods. 118 kidney organoids were categorized into five conditions. Control organoids followed standard culture, while two slow-freezing groups used 10% DMSO (SF1) or commercial freezing media (SF2). Vitrification involved V1 (20% DMSO, 20% Ethylene Glycol with sucrose) and V2 (15% DMSO, 15% Ethylene Glycol). Assessment of viability, functionality, and structural integrity post-thawing revealed notable differences. Vitrification, particularly V1, exhibited superior viability (91% for V1, 26% for V2, 79% for SF1, and 83% for SF2 compared to 99.4% in controls). 3D imaging highlighted distinct nephron segments among groups, emphasizing V1’s efficacy in preserving both podocytes and tubules in kidney organoids. Cisplatin-induced injury revealed a significant reduction in regenerative capacities in organoids cryopreserved by flow-freezing methods, while the V1 method did not show statistical significance compared to the unfrozen controls. This study underscores vitrification, especially with high concentrations of cryoprotectants, as an effective approach for maintaining kidney organoid viability and structure during cryopreservation, offering practical approaches for kidney organoid research. Vitrification (dpeaa)DE-He213 Freezing (dpeaa)DE-He213 Kidney (dpeaa)DE-He213 Organoids (dpeaa)DE-He213 Cryopreservation (dpeaa)DE-He213 Dimethyl sulfoxide (dpeaa)DE-He213 Tabibzadeh, Nahid verfasserin aut Kuraoka, Shohei verfasserin aut Oishi, Haruka verfasserin aut Morizane, Ryuji verfasserin (orcid)0000-0002-0377-5667 aut Enthalten in Cellular and molecular life sciences Springer International Publishing, 1997 81(2024), 1 vom: 18. Juli (DE-627)253390524 (DE-600)1458497-9 1420-9071 nnns volume:81 year:2024 number:1 day:18 month:07 https://dx.doi.org/10.1007/s00018-024-05352-7 X:SPRINGER Resolving-System kostenfrei Volltext SYSFLAG_0 GBV_SPRINGER SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_165 GBV_ILN_170 GBV_ILN_187 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_267 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2005 GBV_ILN_2014 GBV_ILN_2021 GBV_ILN_2050 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 42.15 VZ AR 81 2024 1 18 07
allfieldsSound	10.1007/s00018-024-05352-7 doi (DE-627)SPR056634498 (SPR)s00018-024-05352-7-e DE-627 ger DE-627 rakwb eng 570 610 VZ 42.15 bkl Mashouf, Parham verfasserin aut Cryopreservation of human kidney organoids 2024 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s) 2024 Abstract Recent advances in stem cell research have led to the creation of organoids, miniature replicas of human organs, offering innovative avenues for studying diseases. Kidney organoids, with their ability to replicate complex renal structures, provide a novel platform for investigating kidney diseases and assessing drug efficacy, albeit hindered by labor-intensive generation and batch variations, highlighting the need for tailored cryopreservation methods to enable widespread utilization. Here, we evaluated cryopreservation strategies for kidney organoids by contrasting slow-freezing and vitrification methods. 118 kidney organoids were categorized into five conditions. Control organoids followed standard culture, while two slow-freezing groups used 10% DMSO (SF1) or commercial freezing media (SF2). Vitrification involved V1 (20% DMSO, 20% Ethylene Glycol with sucrose) and V2 (15% DMSO, 15% Ethylene Glycol). Assessment of viability, functionality, and structural integrity post-thawing revealed notable differences. Vitrification, particularly V1, exhibited superior viability (91% for V1, 26% for V2, 79% for SF1, and 83% for SF2 compared to 99.4% in controls). 3D imaging highlighted distinct nephron segments among groups, emphasizing V1’s efficacy in preserving both podocytes and tubules in kidney organoids. Cisplatin-induced injury revealed a significant reduction in regenerative capacities in organoids cryopreserved by flow-freezing methods, while the V1 method did not show statistical significance compared to the unfrozen controls. This study underscores vitrification, especially with high concentrations of cryoprotectants, as an effective approach for maintaining kidney organoid viability and structure during cryopreservation, offering practical approaches for kidney organoid research. Vitrification (dpeaa)DE-He213 Freezing (dpeaa)DE-He213 Kidney (dpeaa)DE-He213 Organoids (dpeaa)DE-He213 Cryopreservation (dpeaa)DE-He213 Dimethyl sulfoxide (dpeaa)DE-He213 Tabibzadeh, Nahid verfasserin aut Kuraoka, Shohei verfasserin aut Oishi, Haruka verfasserin aut Morizane, Ryuji verfasserin (orcid)0000-0002-0377-5667 aut Enthalten in Cellular and molecular life sciences Springer International Publishing, 1997 81(2024), 1 vom: 18. Juli (DE-627)253390524 (DE-600)1458497-9 1420-9071 nnns volume:81 year:2024 number:1 day:18 month:07 https://dx.doi.org/10.1007/s00018-024-05352-7 X:SPRINGER Resolving-System kostenfrei Volltext SYSFLAG_0 GBV_SPRINGER SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_165 GBV_ILN_170 GBV_ILN_187 GBV_ILN_206 GBV_ILN_213 GBV_ILN_230 GBV_ILN_267 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2005 GBV_ILN_2014 GBV_ILN_2021 GBV_ILN_2050 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 42.15 VZ AR 81 2024 1 18 07
language	English
source	Enthalten in Cellular and molecular life sciences 81(2024), 1 vom: 18. Juli volume:81 year:2024 number:1 day:18 month:07
sourceStr	Enthalten in Cellular and molecular life sciences 81(2024), 1 vom: 18. Juli volume:81 year:2024 number:1 day:18 month:07
format_phy_str_mv	Article
institution	findex.gbv.de
topic_facet	Vitrification Freezing Kidney Organoids Cryopreservation Dimethyl sulfoxide
dewey-raw	570
isfreeaccess_bool	true
container_title	Cellular and molecular life sciences
authorswithroles_txt_mv	Mashouf, Parham @@aut@@ Tabibzadeh, Nahid @@aut@@ Kuraoka, Shohei @@aut@@ Oishi, Haruka @@aut@@ Morizane, Ryuji @@aut@@
publishDateDaySort_date	2024-07-18T00:00:00Z
hierarchy_top_id	253390524
dewey-sort	3570
id	SPR056634498
language_de	englisch
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author	Mashouf, Parham
spellingShingle	Mashouf, Parham ddc 570 bkl 42.15 misc Vitrification misc Freezing misc Kidney misc Organoids misc Cryopreservation misc Dimethyl sulfoxide Cryopreservation of human kidney organoids
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topic_title	570 610 VZ 42.15 bkl Cryopreservation of human kidney organoids Vitrification (dpeaa)DE-He213 Freezing (dpeaa)DE-He213 Kidney (dpeaa)DE-He213 Organoids (dpeaa)DE-He213 Cryopreservation (dpeaa)DE-He213 Dimethyl sulfoxide (dpeaa)DE-He213
topic	ddc 570 bkl 42.15 misc Vitrification misc Freezing misc Kidney misc Organoids misc Cryopreservation misc Dimethyl sulfoxide
topic_unstemmed	ddc 570 bkl 42.15 misc Vitrification misc Freezing misc Kidney misc Organoids misc Cryopreservation misc Dimethyl sulfoxide
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title	Cryopreservation of human kidney organoids
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title_full	Cryopreservation of human kidney organoids
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title_sort	cryopreservation of human kidney organoids
title_auth	Cryopreservation of human kidney organoids
abstract	Abstract Recent advances in stem cell research have led to the creation of organoids, miniature replicas of human organs, offering innovative avenues for studying diseases. Kidney organoids, with their ability to replicate complex renal structures, provide a novel platform for investigating kidney diseases and assessing drug efficacy, albeit hindered by labor-intensive generation and batch variations, highlighting the need for tailored cryopreservation methods to enable widespread utilization. Here, we evaluated cryopreservation strategies for kidney organoids by contrasting slow-freezing and vitrification methods. 118 kidney organoids were categorized into five conditions. Control organoids followed standard culture, while two slow-freezing groups used 10% DMSO (SF1) or commercial freezing media (SF2). Vitrification involved V1 (20% DMSO, 20% Ethylene Glycol with sucrose) and V2 (15% DMSO, 15% Ethylene Glycol). Assessment of viability, functionality, and structural integrity post-thawing revealed notable differences. Vitrification, particularly V1, exhibited superior viability (91% for V1, 26% for V2, 79% for SF1, and 83% for SF2 compared to 99.4% in controls). 3D imaging highlighted distinct nephron segments among groups, emphasizing V1’s efficacy in preserving both podocytes and tubules in kidney organoids. Cisplatin-induced injury revealed a significant reduction in regenerative capacities in organoids cryopreserved by flow-freezing methods, while the V1 method did not show statistical significance compared to the unfrozen controls. This study underscores vitrification, especially with high concentrations of cryoprotectants, as an effective approach for maintaining kidney organoid viability and structure during cryopreservation, offering practical approaches for kidney organoid research. © The Author(s) 2024
abstractGer	Abstract Recent advances in stem cell research have led to the creation of organoids, miniature replicas of human organs, offering innovative avenues for studying diseases. Kidney organoids, with their ability to replicate complex renal structures, provide a novel platform for investigating kidney diseases and assessing drug efficacy, albeit hindered by labor-intensive generation and batch variations, highlighting the need for tailored cryopreservation methods to enable widespread utilization. Here, we evaluated cryopreservation strategies for kidney organoids by contrasting slow-freezing and vitrification methods. 118 kidney organoids were categorized into five conditions. Control organoids followed standard culture, while two slow-freezing groups used 10% DMSO (SF1) or commercial freezing media (SF2). Vitrification involved V1 (20% DMSO, 20% Ethylene Glycol with sucrose) and V2 (15% DMSO, 15% Ethylene Glycol). Assessment of viability, functionality, and structural integrity post-thawing revealed notable differences. Vitrification, particularly V1, exhibited superior viability (91% for V1, 26% for V2, 79% for SF1, and 83% for SF2 compared to 99.4% in controls). 3D imaging highlighted distinct nephron segments among groups, emphasizing V1’s efficacy in preserving both podocytes and tubules in kidney organoids. Cisplatin-induced injury revealed a significant reduction in regenerative capacities in organoids cryopreserved by flow-freezing methods, while the V1 method did not show statistical significance compared to the unfrozen controls. This study underscores vitrification, especially with high concentrations of cryoprotectants, as an effective approach for maintaining kidney organoid viability and structure during cryopreservation, offering practical approaches for kidney organoid research. © The Author(s) 2024
abstract_unstemmed	Abstract Recent advances in stem cell research have led to the creation of organoids, miniature replicas of human organs, offering innovative avenues for studying diseases. Kidney organoids, with their ability to replicate complex renal structures, provide a novel platform for investigating kidney diseases and assessing drug efficacy, albeit hindered by labor-intensive generation and batch variations, highlighting the need for tailored cryopreservation methods to enable widespread utilization. Here, we evaluated cryopreservation strategies for kidney organoids by contrasting slow-freezing and vitrification methods. 118 kidney organoids were categorized into five conditions. Control organoids followed standard culture, while two slow-freezing groups used 10% DMSO (SF1) or commercial freezing media (SF2). Vitrification involved V1 (20% DMSO, 20% Ethylene Glycol with sucrose) and V2 (15% DMSO, 15% Ethylene Glycol). Assessment of viability, functionality, and structural integrity post-thawing revealed notable differences. Vitrification, particularly V1, exhibited superior viability (91% for V1, 26% for V2, 79% for SF1, and 83% for SF2 compared to 99.4% in controls). 3D imaging highlighted distinct nephron segments among groups, emphasizing V1’s efficacy in preserving both podocytes and tubules in kidney organoids. Cisplatin-induced injury revealed a significant reduction in regenerative capacities in organoids cryopreserved by flow-freezing methods, while the V1 method did not show statistical significance compared to the unfrozen controls. This study underscores vitrification, especially with high concentrations of cryoprotectants, as an effective approach for maintaining kidney organoid viability and structure during cryopreservation, offering practical approaches for kidney organoid research. © The Author(s) 2024
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title_short	Cryopreservation of human kidney organoids
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Cryopreservation of human kidney organoids

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