Deciphering the Anti-metastatic Efficacy of Semi-purified Indigocarpan by Modulating Matrix Metalloproteinases and Promoting Cell Death
Herbal medicine-based therapies are an essential trait for cancer treatment due to their competency and less side effects. Indigofera aspalathoides Vahl ex DC., Fabaceae, is a well-known traditional medicinal herb that is habituated mainly in South India and Sri Lanka. The previous reports state tha...
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| Autor*in: |
Paramashivam, Sathish Kumar [verfasserIn] Dhiraviam, Kannan Narayanan [verfasserIn] |
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| Format: |
E-Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
2024 |
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| Schlagwörter: |
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| Anmerkung: |
© The Author(s) under exclusive licence to Sociedade Brasileira de Farmacognosia 2024. Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law. |
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| Übergeordnetes Werk: |
Enthalten in: Revista Brasileira de farmacognosia - Springer International Publishing, 1986, 34(2024), 5 vom: 01. Apr., Seite 979-990 |
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| Übergeordnetes Werk: |
volume:34 ; year:2024 ; number:5 ; day:01 ; month:04 ; pages:979-990 |
| Links: |
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| DOI / URN: |
10.1007/s43450-024-00532-9 |
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SPR057268460 |
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| 520 | |a Herbal medicine-based therapies are an essential trait for cancer treatment due to their competency and less side effects. Indigofera aspalathoides Vahl ex DC., Fabaceae, is a well-known traditional medicinal herb that is habituated mainly in South India and Sri Lanka. The previous reports state that the crude plant extract possesses anti-inflammatory, anti-tumor, and anti-oxidant properties, however the effects of bioactive molecules from the plant have not yet been studied. Here, we have investigated the anticancer property of lead biomolecule from I. aspalathoides targeting matrix metalloproteinases (MMP-2 and MMP-9), which inhibits cancer metastasis and promotes cell death in breast cancer (MDA-MB-231) and prostate cancer (PC3) cell lines. A semi-purified lead biomolecule indigocarpan was obtained from ethyl acetate extract using chromatographic techniques. The major active molecule indigocarpan was tested for its cytotoxicity and the $ IC_{50} $ values were found to be 92 µg/ml (MDA-MB-231) and 93 µg/ml (PC3) and 135 µg/ml (A549), respectively, and further, the effect of drug molecule was assessed to study the morphological changes in both cell lines (MDA-MB-231 and PC3) and no such alterations were observed when indigocarpan was treated with L-132 normal cell line. It also demonstrated that indigocarpan exhibits robust anti-metastatic activity as it significantly inhibited cell-cell contact, cell migration, matrix metalloproteinases (MMP2 and MMP9) expression, and activity in both cell lines. Acridine orange/ethidium bromide staining differentiated the live cells, and the drug-induced early and late apoptotic cells in both cancer cells. The intracellular ROS accumulation was evaluated by using a DCFH-DA assay fluorescent probe to determine the apoptotic effects of the indigocarpan. Hence, from our study, partially purified indigocarpan from I. aspalathoides acts as a potent drug that controls cancer metastasis and induces cell death to prevent breast and prostate cancer. Graphical Abstract | ||
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10.1007/s43450-024-00532-9 doi (DE-627)SPR057268460 (SPR)s43450-024-00532-9-e DE-627 ger DE-627 rakwb eng 610 VZ 15,3 ssgn PHARM DE-84 fid Paramashivam, Sathish Kumar verfasserin (orcid)0000-0002-6020-7842 aut Deciphering the Anti-metastatic Efficacy of Semi-purified Indigocarpan by Modulating Matrix Metalloproteinases and Promoting Cell Death 2024 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s) under exclusive licence to Sociedade Brasileira de Farmacognosia 2024. Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law. Herbal medicine-based therapies are an essential trait for cancer treatment due to their competency and less side effects. Indigofera aspalathoides Vahl ex DC., Fabaceae, is a well-known traditional medicinal herb that is habituated mainly in South India and Sri Lanka. The previous reports state that the crude plant extract possesses anti-inflammatory, anti-tumor, and anti-oxidant properties, however the effects of bioactive molecules from the plant have not yet been studied. Here, we have investigated the anticancer property of lead biomolecule from I. aspalathoides targeting matrix metalloproteinases (MMP-2 and MMP-9), which inhibits cancer metastasis and promotes cell death in breast cancer (MDA-MB-231) and prostate cancer (PC3) cell lines. A semi-purified lead biomolecule indigocarpan was obtained from ethyl acetate extract using chromatographic techniques. The major active molecule indigocarpan was tested for its cytotoxicity and the $ IC_{50} $ values were found to be 92 µg/ml (MDA-MB-231) and 93 µg/ml (PC3) and 135 µg/ml (A549), respectively, and further, the effect of drug molecule was assessed to study the morphological changes in both cell lines (MDA-MB-231 and PC3) and no such alterations were observed when indigocarpan was treated with L-132 normal cell line. It also demonstrated that indigocarpan exhibits robust anti-metastatic activity as it significantly inhibited cell-cell contact, cell migration, matrix metalloproteinases (MMP2 and MMP9) expression, and activity in both cell lines. Acridine orange/ethidium bromide staining differentiated the live cells, and the drug-induced early and late apoptotic cells in both cancer cells. The intracellular ROS accumulation was evaluated by using a DCFH-DA assay fluorescent probe to determine the apoptotic effects of the indigocarpan. Hence, from our study, partially purified indigocarpan from I. aspalathoides acts as a potent drug that controls cancer metastasis and induces cell death to prevent breast and prostate cancer. Graphical Abstract Matrix metalloproteinase (dpeaa)DE-He213 Cancer metastasis (dpeaa)DE-He213 Gelatin (dpeaa)DE-He213 Cytotoxicity (dpeaa)DE-He213 ROS (dpeaa)DE-He213 Cell death (dpeaa)DE-He213 Dhiraviam, Kannan Narayanan verfasserin (orcid)0000-0003-4504-0834 aut Enthalten in Revista Brasileira de farmacognosia Springer International Publishing, 1986 34(2024), 5 vom: 01. Apr., Seite 979-990 (DE-627)730275531 (DE-600)2690840-2 1981-528X nnns volume:34 year:2024 number:5 day:01 month:04 pages:979-990 https://dx.doi.org/10.1007/s43450-024-00532-9 X:SPRINGER Resolving-System lizenzpflichtig Volltext SYSFLAG_0 GBV_SPRINGER FID-PHARM SSG-OLC-PHA SSG-OPC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 34 2024 5 01 04 979-990 |
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10.1007/s43450-024-00532-9 doi (DE-627)SPR057268460 (SPR)s43450-024-00532-9-e DE-627 ger DE-627 rakwb eng 610 VZ 15,3 ssgn PHARM DE-84 fid Paramashivam, Sathish Kumar verfasserin (orcid)0000-0002-6020-7842 aut Deciphering the Anti-metastatic Efficacy of Semi-purified Indigocarpan by Modulating Matrix Metalloproteinases and Promoting Cell Death 2024 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s) under exclusive licence to Sociedade Brasileira de Farmacognosia 2024. Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law. Herbal medicine-based therapies are an essential trait for cancer treatment due to their competency and less side effects. Indigofera aspalathoides Vahl ex DC., Fabaceae, is a well-known traditional medicinal herb that is habituated mainly in South India and Sri Lanka. The previous reports state that the crude plant extract possesses anti-inflammatory, anti-tumor, and anti-oxidant properties, however the effects of bioactive molecules from the plant have not yet been studied. Here, we have investigated the anticancer property of lead biomolecule from I. aspalathoides targeting matrix metalloproteinases (MMP-2 and MMP-9), which inhibits cancer metastasis and promotes cell death in breast cancer (MDA-MB-231) and prostate cancer (PC3) cell lines. A semi-purified lead biomolecule indigocarpan was obtained from ethyl acetate extract using chromatographic techniques. The major active molecule indigocarpan was tested for its cytotoxicity and the $ IC_{50} $ values were found to be 92 µg/ml (MDA-MB-231) and 93 µg/ml (PC3) and 135 µg/ml (A549), respectively, and further, the effect of drug molecule was assessed to study the morphological changes in both cell lines (MDA-MB-231 and PC3) and no such alterations were observed when indigocarpan was treated with L-132 normal cell line. It also demonstrated that indigocarpan exhibits robust anti-metastatic activity as it significantly inhibited cell-cell contact, cell migration, matrix metalloproteinases (MMP2 and MMP9) expression, and activity in both cell lines. Acridine orange/ethidium bromide staining differentiated the live cells, and the drug-induced early and late apoptotic cells in both cancer cells. The intracellular ROS accumulation was evaluated by using a DCFH-DA assay fluorescent probe to determine the apoptotic effects of the indigocarpan. Hence, from our study, partially purified indigocarpan from I. aspalathoides acts as a potent drug that controls cancer metastasis and induces cell death to prevent breast and prostate cancer. Graphical Abstract Matrix metalloproteinase (dpeaa)DE-He213 Cancer metastasis (dpeaa)DE-He213 Gelatin (dpeaa)DE-He213 Cytotoxicity (dpeaa)DE-He213 ROS (dpeaa)DE-He213 Cell death (dpeaa)DE-He213 Dhiraviam, Kannan Narayanan verfasserin (orcid)0000-0003-4504-0834 aut Enthalten in Revista Brasileira de farmacognosia Springer International Publishing, 1986 34(2024), 5 vom: 01. Apr., Seite 979-990 (DE-627)730275531 (DE-600)2690840-2 1981-528X nnns volume:34 year:2024 number:5 day:01 month:04 pages:979-990 https://dx.doi.org/10.1007/s43450-024-00532-9 X:SPRINGER Resolving-System lizenzpflichtig Volltext SYSFLAG_0 GBV_SPRINGER FID-PHARM SSG-OLC-PHA SSG-OPC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 34 2024 5 01 04 979-990 |
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10.1007/s43450-024-00532-9 doi (DE-627)SPR057268460 (SPR)s43450-024-00532-9-e DE-627 ger DE-627 rakwb eng 610 VZ 15,3 ssgn PHARM DE-84 fid Paramashivam, Sathish Kumar verfasserin (orcid)0000-0002-6020-7842 aut Deciphering the Anti-metastatic Efficacy of Semi-purified Indigocarpan by Modulating Matrix Metalloproteinases and Promoting Cell Death 2024 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s) under exclusive licence to Sociedade Brasileira de Farmacognosia 2024. Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law. Herbal medicine-based therapies are an essential trait for cancer treatment due to their competency and less side effects. Indigofera aspalathoides Vahl ex DC., Fabaceae, is a well-known traditional medicinal herb that is habituated mainly in South India and Sri Lanka. The previous reports state that the crude plant extract possesses anti-inflammatory, anti-tumor, and anti-oxidant properties, however the effects of bioactive molecules from the plant have not yet been studied. Here, we have investigated the anticancer property of lead biomolecule from I. aspalathoides targeting matrix metalloproteinases (MMP-2 and MMP-9), which inhibits cancer metastasis and promotes cell death in breast cancer (MDA-MB-231) and prostate cancer (PC3) cell lines. A semi-purified lead biomolecule indigocarpan was obtained from ethyl acetate extract using chromatographic techniques. The major active molecule indigocarpan was tested for its cytotoxicity and the $ IC_{50} $ values were found to be 92 µg/ml (MDA-MB-231) and 93 µg/ml (PC3) and 135 µg/ml (A549), respectively, and further, the effect of drug molecule was assessed to study the morphological changes in both cell lines (MDA-MB-231 and PC3) and no such alterations were observed when indigocarpan was treated with L-132 normal cell line. It also demonstrated that indigocarpan exhibits robust anti-metastatic activity as it significantly inhibited cell-cell contact, cell migration, matrix metalloproteinases (MMP2 and MMP9) expression, and activity in both cell lines. Acridine orange/ethidium bromide staining differentiated the live cells, and the drug-induced early and late apoptotic cells in both cancer cells. The intracellular ROS accumulation was evaluated by using a DCFH-DA assay fluorescent probe to determine the apoptotic effects of the indigocarpan. Hence, from our study, partially purified indigocarpan from I. aspalathoides acts as a potent drug that controls cancer metastasis and induces cell death to prevent breast and prostate cancer. Graphical Abstract Matrix metalloproteinase (dpeaa)DE-He213 Cancer metastasis (dpeaa)DE-He213 Gelatin (dpeaa)DE-He213 Cytotoxicity (dpeaa)DE-He213 ROS (dpeaa)DE-He213 Cell death (dpeaa)DE-He213 Dhiraviam, Kannan Narayanan verfasserin (orcid)0000-0003-4504-0834 aut Enthalten in Revista Brasileira de farmacognosia Springer International Publishing, 1986 34(2024), 5 vom: 01. Apr., Seite 979-990 (DE-627)730275531 (DE-600)2690840-2 1981-528X nnns volume:34 year:2024 number:5 day:01 month:04 pages:979-990 https://dx.doi.org/10.1007/s43450-024-00532-9 X:SPRINGER Resolving-System lizenzpflichtig Volltext SYSFLAG_0 GBV_SPRINGER FID-PHARM SSG-OLC-PHA SSG-OPC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 34 2024 5 01 04 979-990 |
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10.1007/s43450-024-00532-9 doi (DE-627)SPR057268460 (SPR)s43450-024-00532-9-e DE-627 ger DE-627 rakwb eng 610 VZ 15,3 ssgn PHARM DE-84 fid Paramashivam, Sathish Kumar verfasserin (orcid)0000-0002-6020-7842 aut Deciphering the Anti-metastatic Efficacy of Semi-purified Indigocarpan by Modulating Matrix Metalloproteinases and Promoting Cell Death 2024 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s) under exclusive licence to Sociedade Brasileira de Farmacognosia 2024. Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law. Herbal medicine-based therapies are an essential trait for cancer treatment due to their competency and less side effects. Indigofera aspalathoides Vahl ex DC., Fabaceae, is a well-known traditional medicinal herb that is habituated mainly in South India and Sri Lanka. The previous reports state that the crude plant extract possesses anti-inflammatory, anti-tumor, and anti-oxidant properties, however the effects of bioactive molecules from the plant have not yet been studied. Here, we have investigated the anticancer property of lead biomolecule from I. aspalathoides targeting matrix metalloproteinases (MMP-2 and MMP-9), which inhibits cancer metastasis and promotes cell death in breast cancer (MDA-MB-231) and prostate cancer (PC3) cell lines. A semi-purified lead biomolecule indigocarpan was obtained from ethyl acetate extract using chromatographic techniques. The major active molecule indigocarpan was tested for its cytotoxicity and the $ IC_{50} $ values were found to be 92 µg/ml (MDA-MB-231) and 93 µg/ml (PC3) and 135 µg/ml (A549), respectively, and further, the effect of drug molecule was assessed to study the morphological changes in both cell lines (MDA-MB-231 and PC3) and no such alterations were observed when indigocarpan was treated with L-132 normal cell line. It also demonstrated that indigocarpan exhibits robust anti-metastatic activity as it significantly inhibited cell-cell contact, cell migration, matrix metalloproteinases (MMP2 and MMP9) expression, and activity in both cell lines. Acridine orange/ethidium bromide staining differentiated the live cells, and the drug-induced early and late apoptotic cells in both cancer cells. The intracellular ROS accumulation was evaluated by using a DCFH-DA assay fluorescent probe to determine the apoptotic effects of the indigocarpan. Hence, from our study, partially purified indigocarpan from I. aspalathoides acts as a potent drug that controls cancer metastasis and induces cell death to prevent breast and prostate cancer. Graphical Abstract Matrix metalloproteinase (dpeaa)DE-He213 Cancer metastasis (dpeaa)DE-He213 Gelatin (dpeaa)DE-He213 Cytotoxicity (dpeaa)DE-He213 ROS (dpeaa)DE-He213 Cell death (dpeaa)DE-He213 Dhiraviam, Kannan Narayanan verfasserin (orcid)0000-0003-4504-0834 aut Enthalten in Revista Brasileira de farmacognosia Springer International Publishing, 1986 34(2024), 5 vom: 01. Apr., Seite 979-990 (DE-627)730275531 (DE-600)2690840-2 1981-528X nnns volume:34 year:2024 number:5 day:01 month:04 pages:979-990 https://dx.doi.org/10.1007/s43450-024-00532-9 X:SPRINGER Resolving-System lizenzpflichtig Volltext SYSFLAG_0 GBV_SPRINGER FID-PHARM SSG-OLC-PHA SSG-OPC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 34 2024 5 01 04 979-990 |
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10.1007/s43450-024-00532-9 doi (DE-627)SPR057268460 (SPR)s43450-024-00532-9-e DE-627 ger DE-627 rakwb eng 610 VZ 15,3 ssgn PHARM DE-84 fid Paramashivam, Sathish Kumar verfasserin (orcid)0000-0002-6020-7842 aut Deciphering the Anti-metastatic Efficacy of Semi-purified Indigocarpan by Modulating Matrix Metalloproteinases and Promoting Cell Death 2024 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s) under exclusive licence to Sociedade Brasileira de Farmacognosia 2024. Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law. Herbal medicine-based therapies are an essential trait for cancer treatment due to their competency and less side effects. Indigofera aspalathoides Vahl ex DC., Fabaceae, is a well-known traditional medicinal herb that is habituated mainly in South India and Sri Lanka. The previous reports state that the crude plant extract possesses anti-inflammatory, anti-tumor, and anti-oxidant properties, however the effects of bioactive molecules from the plant have not yet been studied. Here, we have investigated the anticancer property of lead biomolecule from I. aspalathoides targeting matrix metalloproteinases (MMP-2 and MMP-9), which inhibits cancer metastasis and promotes cell death in breast cancer (MDA-MB-231) and prostate cancer (PC3) cell lines. A semi-purified lead biomolecule indigocarpan was obtained from ethyl acetate extract using chromatographic techniques. The major active molecule indigocarpan was tested for its cytotoxicity and the $ IC_{50} $ values were found to be 92 µg/ml (MDA-MB-231) and 93 µg/ml (PC3) and 135 µg/ml (A549), respectively, and further, the effect of drug molecule was assessed to study the morphological changes in both cell lines (MDA-MB-231 and PC3) and no such alterations were observed when indigocarpan was treated with L-132 normal cell line. It also demonstrated that indigocarpan exhibits robust anti-metastatic activity as it significantly inhibited cell-cell contact, cell migration, matrix metalloproteinases (MMP2 and MMP9) expression, and activity in both cell lines. Acridine orange/ethidium bromide staining differentiated the live cells, and the drug-induced early and late apoptotic cells in both cancer cells. The intracellular ROS accumulation was evaluated by using a DCFH-DA assay fluorescent probe to determine the apoptotic effects of the indigocarpan. Hence, from our study, partially purified indigocarpan from I. aspalathoides acts as a potent drug that controls cancer metastasis and induces cell death to prevent breast and prostate cancer. Graphical Abstract Matrix metalloproteinase (dpeaa)DE-He213 Cancer metastasis (dpeaa)DE-He213 Gelatin (dpeaa)DE-He213 Cytotoxicity (dpeaa)DE-He213 ROS (dpeaa)DE-He213 Cell death (dpeaa)DE-He213 Dhiraviam, Kannan Narayanan verfasserin (orcid)0000-0003-4504-0834 aut Enthalten in Revista Brasileira de farmacognosia Springer International Publishing, 1986 34(2024), 5 vom: 01. Apr., Seite 979-990 (DE-627)730275531 (DE-600)2690840-2 1981-528X nnns volume:34 year:2024 number:5 day:01 month:04 pages:979-990 https://dx.doi.org/10.1007/s43450-024-00532-9 X:SPRINGER Resolving-System lizenzpflichtig Volltext SYSFLAG_0 GBV_SPRINGER FID-PHARM SSG-OLC-PHA SSG-OPC-PHA GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_152 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_250 GBV_ILN_281 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2031 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2039 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2107 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2446 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 34 2024 5 01 04 979-990 |
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Enthalten in Revista Brasileira de farmacognosia 34(2024), 5 vom: 01. Apr., Seite 979-990 volume:34 year:2024 number:5 day:01 month:04 pages:979-990 |
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Enthalten in Revista Brasileira de farmacognosia 34(2024), 5 vom: 01. Apr., Seite 979-990 volume:34 year:2024 number:5 day:01 month:04 pages:979-990 |
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Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a">Herbal medicine-based therapies are an essential trait for cancer treatment due to their competency and less side effects. Indigofera aspalathoides Vahl ex DC., Fabaceae, is a well-known traditional medicinal herb that is habituated mainly in South India and Sri Lanka. The previous reports state that the crude plant extract possesses anti-inflammatory, anti-tumor, and anti-oxidant properties, however the effects of bioactive molecules from the plant have not yet been studied. Here, we have investigated the anticancer property of lead biomolecule from I. aspalathoides targeting matrix metalloproteinases (MMP-2 and MMP-9), which inhibits cancer metastasis and promotes cell death in breast cancer (MDA-MB-231) and prostate cancer (PC3) cell lines. A semi-purified lead biomolecule indigocarpan was obtained from ethyl acetate extract using chromatographic techniques. The major active molecule indigocarpan was tested for its cytotoxicity and the $ IC_{50} $ values were found to be 92 µg/ml (MDA-MB-231) and 93 µg/ml (PC3) and 135 µg/ml (A549), respectively, and further, the effect of drug molecule was assessed to study the morphological changes in both cell lines (MDA-MB-231 and PC3) and no such alterations were observed when indigocarpan was treated with L-132 normal cell line. It also demonstrated that indigocarpan exhibits robust anti-metastatic activity as it significantly inhibited cell-cell contact, cell migration, matrix metalloproteinases (MMP2 and MMP9) expression, and activity in both cell lines. Acridine orange/ethidium bromide staining differentiated the live cells, and the drug-induced early and late apoptotic cells in both cancer cells. The intracellular ROS accumulation was evaluated by using a DCFH-DA assay fluorescent probe to determine the apoptotic effects of the indigocarpan. Hence, from our study, partially purified indigocarpan from I. aspalathoides acts as a potent drug that controls cancer metastasis and induces cell death to prevent breast and prostate cancer. 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Paramashivam, Sathish Kumar |
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Paramashivam, Sathish Kumar ddc 610 ssgn 15,3 fid PHARM misc Matrix metalloproteinase misc Cancer metastasis misc Gelatin misc Cytotoxicity misc ROS misc Cell death Deciphering the Anti-metastatic Efficacy of Semi-purified Indigocarpan by Modulating Matrix Metalloproteinases and Promoting Cell Death |
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610 VZ 15,3 ssgn PHARM DE-84 fid Deciphering the Anti-metastatic Efficacy of Semi-purified Indigocarpan by Modulating Matrix Metalloproteinases and Promoting Cell Death Matrix metalloproteinase (dpeaa)DE-He213 Cancer metastasis (dpeaa)DE-He213 Gelatin (dpeaa)DE-He213 Cytotoxicity (dpeaa)DE-He213 ROS (dpeaa)DE-He213 Cell death (dpeaa)DE-He213 |
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deciphering the anti-metastatic efficacy of semi-purified indigocarpan by modulating matrix metalloproteinases and promoting cell death |
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Deciphering the Anti-metastatic Efficacy of Semi-purified Indigocarpan by Modulating Matrix Metalloproteinases and Promoting Cell Death |
| abstract |
Herbal medicine-based therapies are an essential trait for cancer treatment due to their competency and less side effects. Indigofera aspalathoides Vahl ex DC., Fabaceae, is a well-known traditional medicinal herb that is habituated mainly in South India and Sri Lanka. The previous reports state that the crude plant extract possesses anti-inflammatory, anti-tumor, and anti-oxidant properties, however the effects of bioactive molecules from the plant have not yet been studied. Here, we have investigated the anticancer property of lead biomolecule from I. aspalathoides targeting matrix metalloproteinases (MMP-2 and MMP-9), which inhibits cancer metastasis and promotes cell death in breast cancer (MDA-MB-231) and prostate cancer (PC3) cell lines. A semi-purified lead biomolecule indigocarpan was obtained from ethyl acetate extract using chromatographic techniques. The major active molecule indigocarpan was tested for its cytotoxicity and the $ IC_{50} $ values were found to be 92 µg/ml (MDA-MB-231) and 93 µg/ml (PC3) and 135 µg/ml (A549), respectively, and further, the effect of drug molecule was assessed to study the morphological changes in both cell lines (MDA-MB-231 and PC3) and no such alterations were observed when indigocarpan was treated with L-132 normal cell line. It also demonstrated that indigocarpan exhibits robust anti-metastatic activity as it significantly inhibited cell-cell contact, cell migration, matrix metalloproteinases (MMP2 and MMP9) expression, and activity in both cell lines. Acridine orange/ethidium bromide staining differentiated the live cells, and the drug-induced early and late apoptotic cells in both cancer cells. The intracellular ROS accumulation was evaluated by using a DCFH-DA assay fluorescent probe to determine the apoptotic effects of the indigocarpan. Hence, from our study, partially purified indigocarpan from I. aspalathoides acts as a potent drug that controls cancer metastasis and induces cell death to prevent breast and prostate cancer. Graphical Abstract © The Author(s) under exclusive licence to Sociedade Brasileira de Farmacognosia 2024. Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law. |
| abstractGer |
Herbal medicine-based therapies are an essential trait for cancer treatment due to their competency and less side effects. Indigofera aspalathoides Vahl ex DC., Fabaceae, is a well-known traditional medicinal herb that is habituated mainly in South India and Sri Lanka. The previous reports state that the crude plant extract possesses anti-inflammatory, anti-tumor, and anti-oxidant properties, however the effects of bioactive molecules from the plant have not yet been studied. Here, we have investigated the anticancer property of lead biomolecule from I. aspalathoides targeting matrix metalloproteinases (MMP-2 and MMP-9), which inhibits cancer metastasis and promotes cell death in breast cancer (MDA-MB-231) and prostate cancer (PC3) cell lines. A semi-purified lead biomolecule indigocarpan was obtained from ethyl acetate extract using chromatographic techniques. The major active molecule indigocarpan was tested for its cytotoxicity and the $ IC_{50} $ values were found to be 92 µg/ml (MDA-MB-231) and 93 µg/ml (PC3) and 135 µg/ml (A549), respectively, and further, the effect of drug molecule was assessed to study the morphological changes in both cell lines (MDA-MB-231 and PC3) and no such alterations were observed when indigocarpan was treated with L-132 normal cell line. It also demonstrated that indigocarpan exhibits robust anti-metastatic activity as it significantly inhibited cell-cell contact, cell migration, matrix metalloproteinases (MMP2 and MMP9) expression, and activity in both cell lines. Acridine orange/ethidium bromide staining differentiated the live cells, and the drug-induced early and late apoptotic cells in both cancer cells. The intracellular ROS accumulation was evaluated by using a DCFH-DA assay fluorescent probe to determine the apoptotic effects of the indigocarpan. Hence, from our study, partially purified indigocarpan from I. aspalathoides acts as a potent drug that controls cancer metastasis and induces cell death to prevent breast and prostate cancer. Graphical Abstract © The Author(s) under exclusive licence to Sociedade Brasileira de Farmacognosia 2024. Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law. |
| abstract_unstemmed |
Herbal medicine-based therapies are an essential trait for cancer treatment due to their competency and less side effects. Indigofera aspalathoides Vahl ex DC., Fabaceae, is a well-known traditional medicinal herb that is habituated mainly in South India and Sri Lanka. The previous reports state that the crude plant extract possesses anti-inflammatory, anti-tumor, and anti-oxidant properties, however the effects of bioactive molecules from the plant have not yet been studied. Here, we have investigated the anticancer property of lead biomolecule from I. aspalathoides targeting matrix metalloproteinases (MMP-2 and MMP-9), which inhibits cancer metastasis and promotes cell death in breast cancer (MDA-MB-231) and prostate cancer (PC3) cell lines. A semi-purified lead biomolecule indigocarpan was obtained from ethyl acetate extract using chromatographic techniques. The major active molecule indigocarpan was tested for its cytotoxicity and the $ IC_{50} $ values were found to be 92 µg/ml (MDA-MB-231) and 93 µg/ml (PC3) and 135 µg/ml (A549), respectively, and further, the effect of drug molecule was assessed to study the morphological changes in both cell lines (MDA-MB-231 and PC3) and no such alterations were observed when indigocarpan was treated with L-132 normal cell line. It also demonstrated that indigocarpan exhibits robust anti-metastatic activity as it significantly inhibited cell-cell contact, cell migration, matrix metalloproteinases (MMP2 and MMP9) expression, and activity in both cell lines. Acridine orange/ethidium bromide staining differentiated the live cells, and the drug-induced early and late apoptotic cells in both cancer cells. The intracellular ROS accumulation was evaluated by using a DCFH-DA assay fluorescent probe to determine the apoptotic effects of the indigocarpan. Hence, from our study, partially purified indigocarpan from I. aspalathoides acts as a potent drug that controls cancer metastasis and induces cell death to prevent breast and prostate cancer. Graphical Abstract © The Author(s) under exclusive licence to Sociedade Brasileira de Farmacognosia 2024. Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law. |
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Deciphering the Anti-metastatic Efficacy of Semi-purified Indigocarpan by Modulating Matrix Metalloproteinases and Promoting Cell Death |
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| score |
7.4022713 |