Genetic composition, virulence factors, and antimicrobial resistance profiles of Bacillus cereus and Bacillus subtilis isolates from food vendors in Ondo State, Nigeria: implications for food safety

Background This study investigated Bacillus cereus and Bacillus subtilis from food vendors in Ondo State, Nigeria. Methods A comprehensive whole-genome sequencing (WGS) analysis of Bacillus genomes, including genome assembly, plasmid prediction, species identification, antimicrobial resistance (AMR) gene identification, virulence gene identification, and multilocus sequencing typing, was conducted. Results The genome assembly revealed a B. cereus genome with 87 contigs, a length of 5,798,917 base pairs, and a GC content of 34.79%, whereas B. subtilis had a genome length of 4,238,143 bp and was composed of 253 contigs with a contig L50 of 24, a contig N50 of 55,053, and a GC content of 43.14904%. Plasmid prediction revealed the absence of prominent plasmids in the assembled B. cereus genome, whereas the repUS12 plasmid was recognized with an identity of less than 95.63% for the B. subtilis genome. Species identification via the average nucleotide identity (ANI) calculation confirmed that Bacillus cereus had a 98.97% ANI value, whereas a 98.39% ANI value was confirmed for B. subtilis WAUSV36. AMR genes were identified, with virulence genes such as the alo, cytK, and hbl genes also detected in B. cereus, whereas clpX, codY, purA, and purB genes were detected in B. subtilis. Multiple-locus sequence typing (MLST) revealed that B. cereus belongs to sequence type 73 with 100% identity, identifying housekeeping gene alleles, including glp_13, gmk_8, and ilv_9, whereas B. subtilis belongs to sequence type 130, with the ilvD gene showing a perfect match and the highest allele length of 471 for the housekeeping genes identified. Conclusions This detailed WGS analysis provides valuable insights into the genetic composition, potential virulence factors, and resistance profiles of B. cereus and B. subtilis, enhancing the understanding of their pathogenicity and epidemiology. The genomic analysis of B. cereus and B. subtilis revealed potential genomic applications in the context of food safety. Ausführliche Beschreibung

Gespeichert in:

Autor*in:	Fayanju, Aderonke Mary [verfasserIn] Akinyele, Bamidele Juliet [verfasserIn] Oladejo, Babayemi Olawale [verfasserIn] Osunla, Ayodeji Charles [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2024

Schlagwörter:	Whole-genome sequencing (WGS) Antimicrobial resistance (AMR) Virulence genes Multilocus sequencing typing (MLST) Food safety

Anmerkung:	© The Author(s) 2024

Übergeordnetes Werk:	Enthalten in: Bulletin of the National Research Centre - Springer Berlin Heidelberg, 2018, 48(2024), 1 vom: 16. Sept.
Übergeordnetes Werk:	volume:48 ; year:2024 ; number:1 ; day:16 ; month:09

Links:	Volltext

DOI / URN:	10.1186/s42269-024-01245-8

Katalog-ID:	SPR057345848

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520			\|a Background This study investigated Bacillus cereus and Bacillus subtilis from food vendors in Ondo State, Nigeria. Methods A comprehensive whole-genome sequencing (WGS) analysis of Bacillus genomes, including genome assembly, plasmid prediction, species identification, antimicrobial resistance (AMR) gene identification, virulence gene identification, and multilocus sequencing typing, was conducted. Results The genome assembly revealed a B. cereus genome with 87 contigs, a length of 5,798,917 base pairs, and a GC content of 34.79%, whereas B. subtilis had a genome length of 4,238,143 bp and was composed of 253 contigs with a contig L50 of 24, a contig N50 of 55,053, and a GC content of 43.14904%. Plasmid prediction revealed the absence of prominent plasmids in the assembled B. cereus genome, whereas the repUS12 plasmid was recognized with an identity of less than 95.63% for the B. subtilis genome. Species identification via the average nucleotide identity (ANI) calculation confirmed that Bacillus cereus had a 98.97% ANI value, whereas a 98.39% ANI value was confirmed for B. subtilis WAUSV36. AMR genes were identified, with virulence genes such as the alo, cytK, and hbl genes also detected in B. cereus, whereas clpX, codY, purA, and purB genes were detected in B. subtilis. Multiple-locus sequence typing (MLST) revealed that B. cereus belongs to sequence type 73 with 100% identity, identifying housekeeping gene alleles, including glp_13, gmk_8, and ilv_9, whereas B. subtilis belongs to sequence type 130, with the ilvD gene showing a perfect match and the highest allele length of 471 for the housekeeping genes identified. Conclusions This detailed WGS analysis provides valuable insights into the genetic composition, potential virulence factors, and resistance profiles of B. cereus and B. subtilis, enhancing the understanding of their pathogenicity and epidemiology. The genomic analysis of B. cereus and B. subtilis revealed potential genomic applications in the context of food safety.
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allfields	10.1186/s42269-024-01245-8 doi (DE-627)SPR057345848 (SPR)s42269-024-01245-8-e DE-627 ger DE-627 rakwb eng 500 VZ 500 VZ Fayanju, Aderonke Mary verfasserin (orcid)0009-0006-5246-1425 aut Genetic composition, virulence factors, and antimicrobial resistance profiles of Bacillus cereus and Bacillus subtilis isolates from food vendors in Ondo State, Nigeria: implications for food safety 2024 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s) 2024 Background This study investigated Bacillus cereus and Bacillus subtilis from food vendors in Ondo State, Nigeria. Methods A comprehensive whole-genome sequencing (WGS) analysis of Bacillus genomes, including genome assembly, plasmid prediction, species identification, antimicrobial resistance (AMR) gene identification, virulence gene identification, and multilocus sequencing typing, was conducted. Results The genome assembly revealed a B. cereus genome with 87 contigs, a length of 5,798,917 base pairs, and a GC content of 34.79%, whereas B. subtilis had a genome length of 4,238,143 bp and was composed of 253 contigs with a contig L50 of 24, a contig N50 of 55,053, and a GC content of 43.14904%. Plasmid prediction revealed the absence of prominent plasmids in the assembled B. cereus genome, whereas the repUS12 plasmid was recognized with an identity of less than 95.63% for the B. subtilis genome. Species identification via the average nucleotide identity (ANI) calculation confirmed that Bacillus cereus had a 98.97% ANI value, whereas a 98.39% ANI value was confirmed for B. subtilis WAUSV36. AMR genes were identified, with virulence genes such as the alo, cytK, and hbl genes also detected in B. cereus, whereas clpX, codY, purA, and purB genes were detected in B. subtilis. Multiple-locus sequence typing (MLST) revealed that B. cereus belongs to sequence type 73 with 100% identity, identifying housekeeping gene alleles, including glp_13, gmk_8, and ilv_9, whereas B. subtilis belongs to sequence type 130, with the ilvD gene showing a perfect match and the highest allele length of 471 for the housekeeping genes identified. Conclusions This detailed WGS analysis provides valuable insights into the genetic composition, potential virulence factors, and resistance profiles of B. cereus and B. subtilis, enhancing the understanding of their pathogenicity and epidemiology. The genomic analysis of B. cereus and B. subtilis revealed potential genomic applications in the context of food safety. Whole-genome sequencing (WGS) (dpeaa)DE-He213 Antimicrobial resistance (AMR) (dpeaa)DE-He213 Virulence genes (dpeaa)DE-He213 Multilocus sequencing typing (MLST) (dpeaa)DE-He213 Food safety (dpeaa)DE-He213 Akinyele, Bamidele Juliet verfasserin aut Oladejo, Babayemi Olawale verfasserin aut Osunla, Ayodeji Charles verfasserin aut Enthalten in Bulletin of the National Research Centre Springer Berlin Heidelberg, 2018 48(2024), 1 vom: 16. Sept. (DE-627)1035877007 (DE-600)2946659-3 2522-8307 nnns volume:48 year:2024 number:1 day:16 month:09 https://dx.doi.org/10.1186/s42269-024-01245-8 X:SPRINGER Resolving-System kostenfrei Volltext SYSFLAG_0 GBV_SPRINGER GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2014 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 48 2024 1 16 09
spelling	10.1186/s42269-024-01245-8 doi (DE-627)SPR057345848 (SPR)s42269-024-01245-8-e DE-627 ger DE-627 rakwb eng 500 VZ 500 VZ Fayanju, Aderonke Mary verfasserin (orcid)0009-0006-5246-1425 aut Genetic composition, virulence factors, and antimicrobial resistance profiles of Bacillus cereus and Bacillus subtilis isolates from food vendors in Ondo State, Nigeria: implications for food safety 2024 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s) 2024 Background This study investigated Bacillus cereus and Bacillus subtilis from food vendors in Ondo State, Nigeria. Methods A comprehensive whole-genome sequencing (WGS) analysis of Bacillus genomes, including genome assembly, plasmid prediction, species identification, antimicrobial resistance (AMR) gene identification, virulence gene identification, and multilocus sequencing typing, was conducted. Results The genome assembly revealed a B. cereus genome with 87 contigs, a length of 5,798,917 base pairs, and a GC content of 34.79%, whereas B. subtilis had a genome length of 4,238,143 bp and was composed of 253 contigs with a contig L50 of 24, a contig N50 of 55,053, and a GC content of 43.14904%. Plasmid prediction revealed the absence of prominent plasmids in the assembled B. cereus genome, whereas the repUS12 plasmid was recognized with an identity of less than 95.63% for the B. subtilis genome. Species identification via the average nucleotide identity (ANI) calculation confirmed that Bacillus cereus had a 98.97% ANI value, whereas a 98.39% ANI value was confirmed for B. subtilis WAUSV36. AMR genes were identified, with virulence genes such as the alo, cytK, and hbl genes also detected in B. cereus, whereas clpX, codY, purA, and purB genes were detected in B. subtilis. Multiple-locus sequence typing (MLST) revealed that B. cereus belongs to sequence type 73 with 100% identity, identifying housekeeping gene alleles, including glp_13, gmk_8, and ilv_9, whereas B. subtilis belongs to sequence type 130, with the ilvD gene showing a perfect match and the highest allele length of 471 for the housekeeping genes identified. Conclusions This detailed WGS analysis provides valuable insights into the genetic composition, potential virulence factors, and resistance profiles of B. cereus and B. subtilis, enhancing the understanding of their pathogenicity and epidemiology. The genomic analysis of B. cereus and B. subtilis revealed potential genomic applications in the context of food safety. Whole-genome sequencing (WGS) (dpeaa)DE-He213 Antimicrobial resistance (AMR) (dpeaa)DE-He213 Virulence genes (dpeaa)DE-He213 Multilocus sequencing typing (MLST) (dpeaa)DE-He213 Food safety (dpeaa)DE-He213 Akinyele, Bamidele Juliet verfasserin aut Oladejo, Babayemi Olawale verfasserin aut Osunla, Ayodeji Charles verfasserin aut Enthalten in Bulletin of the National Research Centre Springer Berlin Heidelberg, 2018 48(2024), 1 vom: 16. Sept. (DE-627)1035877007 (DE-600)2946659-3 2522-8307 nnns volume:48 year:2024 number:1 day:16 month:09 https://dx.doi.org/10.1186/s42269-024-01245-8 X:SPRINGER Resolving-System kostenfrei Volltext SYSFLAG_0 GBV_SPRINGER GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2014 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 48 2024 1 16 09
allfields_unstemmed	10.1186/s42269-024-01245-8 doi (DE-627)SPR057345848 (SPR)s42269-024-01245-8-e DE-627 ger DE-627 rakwb eng 500 VZ 500 VZ Fayanju, Aderonke Mary verfasserin (orcid)0009-0006-5246-1425 aut Genetic composition, virulence factors, and antimicrobial resistance profiles of Bacillus cereus and Bacillus subtilis isolates from food vendors in Ondo State, Nigeria: implications for food safety 2024 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s) 2024 Background This study investigated Bacillus cereus and Bacillus subtilis from food vendors in Ondo State, Nigeria. Methods A comprehensive whole-genome sequencing (WGS) analysis of Bacillus genomes, including genome assembly, plasmid prediction, species identification, antimicrobial resistance (AMR) gene identification, virulence gene identification, and multilocus sequencing typing, was conducted. Results The genome assembly revealed a B. cereus genome with 87 contigs, a length of 5,798,917 base pairs, and a GC content of 34.79%, whereas B. subtilis had a genome length of 4,238,143 bp and was composed of 253 contigs with a contig L50 of 24, a contig N50 of 55,053, and a GC content of 43.14904%. Plasmid prediction revealed the absence of prominent plasmids in the assembled B. cereus genome, whereas the repUS12 plasmid was recognized with an identity of less than 95.63% for the B. subtilis genome. Species identification via the average nucleotide identity (ANI) calculation confirmed that Bacillus cereus had a 98.97% ANI value, whereas a 98.39% ANI value was confirmed for B. subtilis WAUSV36. AMR genes were identified, with virulence genes such as the alo, cytK, and hbl genes also detected in B. cereus, whereas clpX, codY, purA, and purB genes were detected in B. subtilis. Multiple-locus sequence typing (MLST) revealed that B. cereus belongs to sequence type 73 with 100% identity, identifying housekeeping gene alleles, including glp_13, gmk_8, and ilv_9, whereas B. subtilis belongs to sequence type 130, with the ilvD gene showing a perfect match and the highest allele length of 471 for the housekeeping genes identified. Conclusions This detailed WGS analysis provides valuable insights into the genetic composition, potential virulence factors, and resistance profiles of B. cereus and B. subtilis, enhancing the understanding of their pathogenicity and epidemiology. The genomic analysis of B. cereus and B. subtilis revealed potential genomic applications in the context of food safety. Whole-genome sequencing (WGS) (dpeaa)DE-He213 Antimicrobial resistance (AMR) (dpeaa)DE-He213 Virulence genes (dpeaa)DE-He213 Multilocus sequencing typing (MLST) (dpeaa)DE-He213 Food safety (dpeaa)DE-He213 Akinyele, Bamidele Juliet verfasserin aut Oladejo, Babayemi Olawale verfasserin aut Osunla, Ayodeji Charles verfasserin aut Enthalten in Bulletin of the National Research Centre Springer Berlin Heidelberg, 2018 48(2024), 1 vom: 16. Sept. (DE-627)1035877007 (DE-600)2946659-3 2522-8307 nnns volume:48 year:2024 number:1 day:16 month:09 https://dx.doi.org/10.1186/s42269-024-01245-8 X:SPRINGER Resolving-System kostenfrei Volltext SYSFLAG_0 GBV_SPRINGER GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2014 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 48 2024 1 16 09
allfieldsGer	10.1186/s42269-024-01245-8 doi (DE-627)SPR057345848 (SPR)s42269-024-01245-8-e DE-627 ger DE-627 rakwb eng 500 VZ 500 VZ Fayanju, Aderonke Mary verfasserin (orcid)0009-0006-5246-1425 aut Genetic composition, virulence factors, and antimicrobial resistance profiles of Bacillus cereus and Bacillus subtilis isolates from food vendors in Ondo State, Nigeria: implications for food safety 2024 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s) 2024 Background This study investigated Bacillus cereus and Bacillus subtilis from food vendors in Ondo State, Nigeria. Methods A comprehensive whole-genome sequencing (WGS) analysis of Bacillus genomes, including genome assembly, plasmid prediction, species identification, antimicrobial resistance (AMR) gene identification, virulence gene identification, and multilocus sequencing typing, was conducted. Results The genome assembly revealed a B. cereus genome with 87 contigs, a length of 5,798,917 base pairs, and a GC content of 34.79%, whereas B. subtilis had a genome length of 4,238,143 bp and was composed of 253 contigs with a contig L50 of 24, a contig N50 of 55,053, and a GC content of 43.14904%. Plasmid prediction revealed the absence of prominent plasmids in the assembled B. cereus genome, whereas the repUS12 plasmid was recognized with an identity of less than 95.63% for the B. subtilis genome. Species identification via the average nucleotide identity (ANI) calculation confirmed that Bacillus cereus had a 98.97% ANI value, whereas a 98.39% ANI value was confirmed for B. subtilis WAUSV36. AMR genes were identified, with virulence genes such as the alo, cytK, and hbl genes also detected in B. cereus, whereas clpX, codY, purA, and purB genes were detected in B. subtilis. Multiple-locus sequence typing (MLST) revealed that B. cereus belongs to sequence type 73 with 100% identity, identifying housekeeping gene alleles, including glp_13, gmk_8, and ilv_9, whereas B. subtilis belongs to sequence type 130, with the ilvD gene showing a perfect match and the highest allele length of 471 for the housekeeping genes identified. Conclusions This detailed WGS analysis provides valuable insights into the genetic composition, potential virulence factors, and resistance profiles of B. cereus and B. subtilis, enhancing the understanding of their pathogenicity and epidemiology. The genomic analysis of B. cereus and B. subtilis revealed potential genomic applications in the context of food safety. Whole-genome sequencing (WGS) (dpeaa)DE-He213 Antimicrobial resistance (AMR) (dpeaa)DE-He213 Virulence genes (dpeaa)DE-He213 Multilocus sequencing typing (MLST) (dpeaa)DE-He213 Food safety (dpeaa)DE-He213 Akinyele, Bamidele Juliet verfasserin aut Oladejo, Babayemi Olawale verfasserin aut Osunla, Ayodeji Charles verfasserin aut Enthalten in Bulletin of the National Research Centre Springer Berlin Heidelberg, 2018 48(2024), 1 vom: 16. Sept. (DE-627)1035877007 (DE-600)2946659-3 2522-8307 nnns volume:48 year:2024 number:1 day:16 month:09 https://dx.doi.org/10.1186/s42269-024-01245-8 X:SPRINGER Resolving-System kostenfrei Volltext SYSFLAG_0 GBV_SPRINGER GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2014 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 48 2024 1 16 09
allfieldsSound	10.1186/s42269-024-01245-8 doi (DE-627)SPR057345848 (SPR)s42269-024-01245-8-e DE-627 ger DE-627 rakwb eng 500 VZ 500 VZ Fayanju, Aderonke Mary verfasserin (orcid)0009-0006-5246-1425 aut Genetic composition, virulence factors, and antimicrobial resistance profiles of Bacillus cereus and Bacillus subtilis isolates from food vendors in Ondo State, Nigeria: implications for food safety 2024 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s) 2024 Background This study investigated Bacillus cereus and Bacillus subtilis from food vendors in Ondo State, Nigeria. Methods A comprehensive whole-genome sequencing (WGS) analysis of Bacillus genomes, including genome assembly, plasmid prediction, species identification, antimicrobial resistance (AMR) gene identification, virulence gene identification, and multilocus sequencing typing, was conducted. Results The genome assembly revealed a B. cereus genome with 87 contigs, a length of 5,798,917 base pairs, and a GC content of 34.79%, whereas B. subtilis had a genome length of 4,238,143 bp and was composed of 253 contigs with a contig L50 of 24, a contig N50 of 55,053, and a GC content of 43.14904%. Plasmid prediction revealed the absence of prominent plasmids in the assembled B. cereus genome, whereas the repUS12 plasmid was recognized with an identity of less than 95.63% for the B. subtilis genome. Species identification via the average nucleotide identity (ANI) calculation confirmed that Bacillus cereus had a 98.97% ANI value, whereas a 98.39% ANI value was confirmed for B. subtilis WAUSV36. AMR genes were identified, with virulence genes such as the alo, cytK, and hbl genes also detected in B. cereus, whereas clpX, codY, purA, and purB genes were detected in B. subtilis. Multiple-locus sequence typing (MLST) revealed that B. cereus belongs to sequence type 73 with 100% identity, identifying housekeeping gene alleles, including glp_13, gmk_8, and ilv_9, whereas B. subtilis belongs to sequence type 130, with the ilvD gene showing a perfect match and the highest allele length of 471 for the housekeeping genes identified. Conclusions This detailed WGS analysis provides valuable insights into the genetic composition, potential virulence factors, and resistance profiles of B. cereus and B. subtilis, enhancing the understanding of their pathogenicity and epidemiology. The genomic analysis of B. cereus and B. subtilis revealed potential genomic applications in the context of food safety. Whole-genome sequencing (WGS) (dpeaa)DE-He213 Antimicrobial resistance (AMR) (dpeaa)DE-He213 Virulence genes (dpeaa)DE-He213 Multilocus sequencing typing (MLST) (dpeaa)DE-He213 Food safety (dpeaa)DE-He213 Akinyele, Bamidele Juliet verfasserin aut Oladejo, Babayemi Olawale verfasserin aut Osunla, Ayodeji Charles verfasserin aut Enthalten in Bulletin of the National Research Centre Springer Berlin Heidelberg, 2018 48(2024), 1 vom: 16. Sept. (DE-627)1035877007 (DE-600)2946659-3 2522-8307 nnns volume:48 year:2024 number:1 day:16 month:09 https://dx.doi.org/10.1186/s42269-024-01245-8 X:SPRINGER Resolving-System kostenfrei Volltext SYSFLAG_0 GBV_SPRINGER GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_2005 GBV_ILN_2009 GBV_ILN_2014 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2111 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 48 2024 1 16 09
language	English
source	Enthalten in Bulletin of the National Research Centre 48(2024), 1 vom: 16. Sept. volume:48 year:2024 number:1 day:16 month:09
sourceStr	Enthalten in Bulletin of the National Research Centre 48(2024), 1 vom: 16. Sept. volume:48 year:2024 number:1 day:16 month:09
format_phy_str_mv	Article
institution	findex.gbv.de
topic_facet	Whole-genome sequencing (WGS) Antimicrobial resistance (AMR) Virulence genes Multilocus sequencing typing (MLST) Food safety
dewey-raw	500
isfreeaccess_bool	true
container_title	Bulletin of the National Research Centre
authorswithroles_txt_mv	Fayanju, Aderonke Mary @@aut@@ Akinyele, Bamidele Juliet @@aut@@ Oladejo, Babayemi Olawale @@aut@@ Osunla, Ayodeji Charles @@aut@@
publishDateDaySort_date	2024-09-16T00:00:00Z
hierarchy_top_id	1035877007
dewey-sort	3500
id	SPR057345848
language_de	englisch
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Methods A comprehensive whole-genome sequencing (WGS) analysis of Bacillus genomes, including genome assembly, plasmid prediction, species identification, antimicrobial resistance (AMR) gene identification, virulence gene identification, and multilocus sequencing typing, was conducted. Results The genome assembly revealed a B. cereus genome with 87 contigs, a length of 5,798,917 base pairs, and a GC content of 34.79%, whereas B. subtilis had a genome length of 4,238,143 bp and was composed of 253 contigs with a contig L50 of 24, a contig N50 of 55,053, and a GC content of 43.14904%. Plasmid prediction revealed the absence of prominent plasmids in the assembled B. cereus genome, whereas the repUS12 plasmid was recognized with an identity of less than 95.63% for the B. subtilis genome. Species identification via the average nucleotide identity (ANI) calculation confirmed that Bacillus cereus had a 98.97% ANI value, whereas a 98.39% ANI value was confirmed for B. subtilis WAUSV36. AMR genes were identified, with virulence genes such as the alo, cytK, and hbl genes also detected in B. cereus, whereas clpX, codY, purA, and purB genes were detected in B. subtilis. Multiple-locus sequence typing (MLST) revealed that B. cereus belongs to sequence type 73 with 100% identity, identifying housekeeping gene alleles, including glp_13, gmk_8, and ilv_9, whereas B. subtilis belongs to sequence type 130, with the ilvD gene showing a perfect match and the highest allele length of 471 for the housekeeping genes identified. Conclusions This detailed WGS analysis provides valuable insights into the genetic composition, potential virulence factors, and resistance profiles of B. cereus and B. subtilis, enhancing the understanding of their pathogenicity and epidemiology. 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author	Fayanju, Aderonke Mary
spellingShingle	Fayanju, Aderonke Mary ddc 500 misc Whole-genome sequencing (WGS) misc Antimicrobial resistance (AMR) misc Virulence genes misc Multilocus sequencing typing (MLST) misc Food safety Genetic composition, virulence factors, and antimicrobial resistance profiles of Bacillus cereus and Bacillus subtilis isolates from food vendors in Ondo State, Nigeria: implications for food safety
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topic_title	500 VZ Genetic composition, virulence factors, and antimicrobial resistance profiles of Bacillus cereus and Bacillus subtilis isolates from food vendors in Ondo State, Nigeria: implications for food safety Whole-genome sequencing (WGS) (dpeaa)DE-He213 Antimicrobial resistance (AMR) (dpeaa)DE-He213 Virulence genes (dpeaa)DE-He213 Multilocus sequencing typing (MLST) (dpeaa)DE-He213 Food safety (dpeaa)DE-He213
topic	ddc 500 misc Whole-genome sequencing (WGS) misc Antimicrobial resistance (AMR) misc Virulence genes misc Multilocus sequencing typing (MLST) misc Food safety
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title	Genetic composition, virulence factors, and antimicrobial resistance profiles of Bacillus cereus and Bacillus subtilis isolates from food vendors in Ondo State, Nigeria: implications for food safety
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title_full	Genetic composition, virulence factors, and antimicrobial resistance profiles of Bacillus cereus and Bacillus subtilis isolates from food vendors in Ondo State, Nigeria: implications for food safety
author_sort	Fayanju, Aderonke Mary
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author_browse	Fayanju, Aderonke Mary Akinyele, Bamidele Juliet Oladejo, Babayemi Olawale Osunla, Ayodeji Charles
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title_sort	genetic composition, virulence factors, and antimicrobial resistance profiles of bacillus cereus and bacillus subtilis isolates from food vendors in ondo state, nigeria: implications for food safety
title_auth	Genetic composition, virulence factors, and antimicrobial resistance profiles of Bacillus cereus and Bacillus subtilis isolates from food vendors in Ondo State, Nigeria: implications for food safety
abstract	Background This study investigated Bacillus cereus and Bacillus subtilis from food vendors in Ondo State, Nigeria. Methods A comprehensive whole-genome sequencing (WGS) analysis of Bacillus genomes, including genome assembly, plasmid prediction, species identification, antimicrobial resistance (AMR) gene identification, virulence gene identification, and multilocus sequencing typing, was conducted. Results The genome assembly revealed a B. cereus genome with 87 contigs, a length of 5,798,917 base pairs, and a GC content of 34.79%, whereas B. subtilis had a genome length of 4,238,143 bp and was composed of 253 contigs with a contig L50 of 24, a contig N50 of 55,053, and a GC content of 43.14904%. Plasmid prediction revealed the absence of prominent plasmids in the assembled B. cereus genome, whereas the repUS12 plasmid was recognized with an identity of less than 95.63% for the B. subtilis genome. Species identification via the average nucleotide identity (ANI) calculation confirmed that Bacillus cereus had a 98.97% ANI value, whereas a 98.39% ANI value was confirmed for B. subtilis WAUSV36. AMR genes were identified, with virulence genes such as the alo, cytK, and hbl genes also detected in B. cereus, whereas clpX, codY, purA, and purB genes were detected in B. subtilis. Multiple-locus sequence typing (MLST) revealed that B. cereus belongs to sequence type 73 with 100% identity, identifying housekeeping gene alleles, including glp_13, gmk_8, and ilv_9, whereas B. subtilis belongs to sequence type 130, with the ilvD gene showing a perfect match and the highest allele length of 471 for the housekeeping genes identified. Conclusions This detailed WGS analysis provides valuable insights into the genetic composition, potential virulence factors, and resistance profiles of B. cereus and B. subtilis, enhancing the understanding of their pathogenicity and epidemiology. The genomic analysis of B. cereus and B. subtilis revealed potential genomic applications in the context of food safety. © The Author(s) 2024
abstractGer	Background This study investigated Bacillus cereus and Bacillus subtilis from food vendors in Ondo State, Nigeria. Methods A comprehensive whole-genome sequencing (WGS) analysis of Bacillus genomes, including genome assembly, plasmid prediction, species identification, antimicrobial resistance (AMR) gene identification, virulence gene identification, and multilocus sequencing typing, was conducted. Results The genome assembly revealed a B. cereus genome with 87 contigs, a length of 5,798,917 base pairs, and a GC content of 34.79%, whereas B. subtilis had a genome length of 4,238,143 bp and was composed of 253 contigs with a contig L50 of 24, a contig N50 of 55,053, and a GC content of 43.14904%. Plasmid prediction revealed the absence of prominent plasmids in the assembled B. cereus genome, whereas the repUS12 plasmid was recognized with an identity of less than 95.63% for the B. subtilis genome. Species identification via the average nucleotide identity (ANI) calculation confirmed that Bacillus cereus had a 98.97% ANI value, whereas a 98.39% ANI value was confirmed for B. subtilis WAUSV36. AMR genes were identified, with virulence genes such as the alo, cytK, and hbl genes also detected in B. cereus, whereas clpX, codY, purA, and purB genes were detected in B. subtilis. Multiple-locus sequence typing (MLST) revealed that B. cereus belongs to sequence type 73 with 100% identity, identifying housekeeping gene alleles, including glp_13, gmk_8, and ilv_9, whereas B. subtilis belongs to sequence type 130, with the ilvD gene showing a perfect match and the highest allele length of 471 for the housekeeping genes identified. Conclusions This detailed WGS analysis provides valuable insights into the genetic composition, potential virulence factors, and resistance profiles of B. cereus and B. subtilis, enhancing the understanding of their pathogenicity and epidemiology. The genomic analysis of B. cereus and B. subtilis revealed potential genomic applications in the context of food safety. © The Author(s) 2024
abstract_unstemmed	Background This study investigated Bacillus cereus and Bacillus subtilis from food vendors in Ondo State, Nigeria. Methods A comprehensive whole-genome sequencing (WGS) analysis of Bacillus genomes, including genome assembly, plasmid prediction, species identification, antimicrobial resistance (AMR) gene identification, virulence gene identification, and multilocus sequencing typing, was conducted. Results The genome assembly revealed a B. cereus genome with 87 contigs, a length of 5,798,917 base pairs, and a GC content of 34.79%, whereas B. subtilis had a genome length of 4,238,143 bp and was composed of 253 contigs with a contig L50 of 24, a contig N50 of 55,053, and a GC content of 43.14904%. Plasmid prediction revealed the absence of prominent plasmids in the assembled B. cereus genome, whereas the repUS12 plasmid was recognized with an identity of less than 95.63% for the B. subtilis genome. Species identification via the average nucleotide identity (ANI) calculation confirmed that Bacillus cereus had a 98.97% ANI value, whereas a 98.39% ANI value was confirmed for B. subtilis WAUSV36. AMR genes were identified, with virulence genes such as the alo, cytK, and hbl genes also detected in B. cereus, whereas clpX, codY, purA, and purB genes were detected in B. subtilis. Multiple-locus sequence typing (MLST) revealed that B. cereus belongs to sequence type 73 with 100% identity, identifying housekeeping gene alleles, including glp_13, gmk_8, and ilv_9, whereas B. subtilis belongs to sequence type 130, with the ilvD gene showing a perfect match and the highest allele length of 471 for the housekeeping genes identified. Conclusions This detailed WGS analysis provides valuable insights into the genetic composition, potential virulence factors, and resistance profiles of B. cereus and B. subtilis, enhancing the understanding of their pathogenicity and epidemiology. The genomic analysis of B. cereus and B. subtilis revealed potential genomic applications in the context of food safety. © The Author(s) 2024
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container_issue	1
title_short	Genetic composition, virulence factors, and antimicrobial resistance profiles of Bacillus cereus and Bacillus subtilis isolates from food vendors in Ondo State, Nigeria: implications for food safety
url	https://dx.doi.org/10.1186/s42269-024-01245-8
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author2	Akinyele, Bamidele Juliet Oladejo, Babayemi Olawale Osunla, Ayodeji Charles
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Genetic composition, virulence factors, and antimicrobial resistance profiles of Bacillus cereus and Bacillus subtilis isolates from food vendors in Ondo State, Nigeria: implications for food safety

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