Primary structure and differential expression of glutamine synthetase genes in nodules, roots and leaves of Phaseolus vulgaris

Abstract In plants, glutamine synthetase (GS) is the enzyme primarily responsible for the assimilation of ammonia into organic nitrogen. In Phaseolus vulgaris a number of isoenzymic forms of GS are found, each of which consists of eight subunits of mol. wt 41 000‐45 000. The GS subunits of P. vulgar...
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Autor*in:	Gebhardt, Christiane [verfasserIn] Oliver, Jane E. [verfasserIn] Forde, Brian G. [verfasserIn] Saarelainen, Ritva [verfasserIn] Miflin, Benjamin J. [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	1986

Schlagwörter:	glutamine synthetase gene expression cDNA sequence amino acid sequence Phaseolus vulgaris

Anmerkung:	© European Molecular Biology Organization 1986

Übergeordnetes Werk:	Enthalten in: The EMBO Journal - Nature Publishing Group UK, 2023, 5(1986), 7 vom: 01. Juli, Seite 1429-1435
Übergeordnetes Werk:	volume:5 ; year:1986 ; number:7 ; day:01 ; month:07 ; pages:1429-1435

Links:	Volltext

DOI / URN:	10.1002/j.1460-2075.1986.tb04379.x

Katalog-ID:	SPR057561168

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520			\|a Abstract In plants, glutamine synthetase (GS) is the enzyme primarily responsible for the assimilation of ammonia into organic nitrogen. In Phaseolus vulgaris a number of isoenzymic forms of GS are found, each of which consists of eight subunits of mol. wt 41 000‐45 000. The GS subunits of P. vulgaris have previously been shown to be encoded by a small multigene family and a partial cDNA clone for a nodule‐specific GS subunit has been obtained. We report here the isolation and nucleotide sequencing of two essentially full‐length GS cDNA clones (pR‐1 and pR‐2) from a root cDNA library and the deduced amino acid sequences of the corresponding GS subunits (355 amino acid residues each). The coding sequences of pR‐1 and pR‐2 are closely related (80% nucleotide homology, 88% amino acid homology), but their 5′‐ and 3′‐untranslated regions have diverged almost completely. Both pR‐1 and pR‐2 are related to, but distinct from, the nodule GS clone, pcPvNGS‐01 (or pN‐1). Hybridization to genomic Southern blots showed that the three GS mRNAs are encoded by three seperate genes and indicated the existence of a fourth class of GS gene. An S1 nuclease protection assay demonstrated the presence of R‐1 and R‐2 mRNA in both roots and leaves and confirmed that expression of the N‐1 gene is nodule‐specific. Expression of the R‐1 and R‐2 genes in the roots did not change significantly during nodulation. However, only the R‐1 gene is expressed in the nodules themselves, indicating that the R‐2 gene is specifically repressed during nodule development.
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allfields	10.1002/j.1460-2075.1986.tb04379.x doi (DE-627)SPR057561168 (SPR)j.1460-2075.1986.tb04379.x-e DE-627 ger DE-627 rakwb eng Gebhardt, Christiane verfasserin aut Primary structure and differential expression of glutamine synthetase genes in nodules, roots and leaves of Phaseolus vulgaris 1986 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © European Molecular Biology Organization 1986 Abstract In plants, glutamine synthetase (GS) is the enzyme primarily responsible for the assimilation of ammonia into organic nitrogen. In Phaseolus vulgaris a number of isoenzymic forms of GS are found, each of which consists of eight subunits of mol. wt 41 000‐45 000. The GS subunits of P. vulgaris have previously been shown to be encoded by a small multigene family and a partial cDNA clone for a nodule‐specific GS subunit has been obtained. We report here the isolation and nucleotide sequencing of two essentially full‐length GS cDNA clones (pR‐1 and pR‐2) from a root cDNA library and the deduced amino acid sequences of the corresponding GS subunits (355 amino acid residues each). The coding sequences of pR‐1 and pR‐2 are closely related (80% nucleotide homology, 88% amino acid homology), but their 5′‐ and 3′‐untranslated regions have diverged almost completely. Both pR‐1 and pR‐2 are related to, but distinct from, the nodule GS clone, pcPvNGS‐01 (or pN‐1). Hybridization to genomic Southern blots showed that the three GS mRNAs are encoded by three seperate genes and indicated the existence of a fourth class of GS gene. An S1 nuclease protection assay demonstrated the presence of R‐1 and R‐2 mRNA in both roots and leaves and confirmed that expression of the N‐1 gene is nodule‐specific. Expression of the R‐1 and R‐2 genes in the roots did not change significantly during nodulation. However, only the R‐1 gene is expressed in the nodules themselves, indicating that the R‐2 gene is specifically repressed during nodule development. glutamine synthetase (dpeaa)DE-He213 gene expression (dpeaa)DE-He213 cDNA sequence (dpeaa)DE-He213 amino acid sequence (dpeaa)DE-He213 Phaseolus vulgaris (dpeaa)DE-He213 Oliver, Jane E. verfasserin aut Forde, Brian G. verfasserin aut Saarelainen, Ritva verfasserin aut Miflin, Benjamin J. verfasserin aut Enthalten in The EMBO Journal Nature Publishing Group UK, 2023 5(1986), 7 vom: 01. Juli, Seite 1429-1435 (DE-627)266022529 (DE-600)1467419-1 1460-2075 nnns volume:5 year:1986 number:7 day:01 month:07 pages:1429-1435 https://dx.doi.org/10.1002/j.1460-2075.1986.tb04379.x X:SPRINGER Resolving-System lizenzpflichtig Volltext SYSFLAG_0 GBV_SPRINGER GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_72 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_252 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_2021 GBV_ILN_2050 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 5 1986 7 01 07 1429-1435
spelling	10.1002/j.1460-2075.1986.tb04379.x doi (DE-627)SPR057561168 (SPR)j.1460-2075.1986.tb04379.x-e DE-627 ger DE-627 rakwb eng Gebhardt, Christiane verfasserin aut Primary structure and differential expression of glutamine synthetase genes in nodules, roots and leaves of Phaseolus vulgaris 1986 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © European Molecular Biology Organization 1986 Abstract In plants, glutamine synthetase (GS) is the enzyme primarily responsible for the assimilation of ammonia into organic nitrogen. In Phaseolus vulgaris a number of isoenzymic forms of GS are found, each of which consists of eight subunits of mol. wt 41 000‐45 000. The GS subunits of P. vulgaris have previously been shown to be encoded by a small multigene family and a partial cDNA clone for a nodule‐specific GS subunit has been obtained. We report here the isolation and nucleotide sequencing of two essentially full‐length GS cDNA clones (pR‐1 and pR‐2) from a root cDNA library and the deduced amino acid sequences of the corresponding GS subunits (355 amino acid residues each). The coding sequences of pR‐1 and pR‐2 are closely related (80% nucleotide homology, 88% amino acid homology), but their 5′‐ and 3′‐untranslated regions have diverged almost completely. Both pR‐1 and pR‐2 are related to, but distinct from, the nodule GS clone, pcPvNGS‐01 (or pN‐1). Hybridization to genomic Southern blots showed that the three GS mRNAs are encoded by three seperate genes and indicated the existence of a fourth class of GS gene. An S1 nuclease protection assay demonstrated the presence of R‐1 and R‐2 mRNA in both roots and leaves and confirmed that expression of the N‐1 gene is nodule‐specific. Expression of the R‐1 and R‐2 genes in the roots did not change significantly during nodulation. However, only the R‐1 gene is expressed in the nodules themselves, indicating that the R‐2 gene is specifically repressed during nodule development. glutamine synthetase (dpeaa)DE-He213 gene expression (dpeaa)DE-He213 cDNA sequence (dpeaa)DE-He213 amino acid sequence (dpeaa)DE-He213 Phaseolus vulgaris (dpeaa)DE-He213 Oliver, Jane E. verfasserin aut Forde, Brian G. verfasserin aut Saarelainen, Ritva verfasserin aut Miflin, Benjamin J. verfasserin aut Enthalten in The EMBO Journal Nature Publishing Group UK, 2023 5(1986), 7 vom: 01. Juli, Seite 1429-1435 (DE-627)266022529 (DE-600)1467419-1 1460-2075 nnns volume:5 year:1986 number:7 day:01 month:07 pages:1429-1435 https://dx.doi.org/10.1002/j.1460-2075.1986.tb04379.x X:SPRINGER Resolving-System lizenzpflichtig Volltext SYSFLAG_0 GBV_SPRINGER GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_72 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_252 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_2021 GBV_ILN_2050 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 5 1986 7 01 07 1429-1435
allfields_unstemmed	10.1002/j.1460-2075.1986.tb04379.x doi (DE-627)SPR057561168 (SPR)j.1460-2075.1986.tb04379.x-e DE-627 ger DE-627 rakwb eng Gebhardt, Christiane verfasserin aut Primary structure and differential expression of glutamine synthetase genes in nodules, roots and leaves of Phaseolus vulgaris 1986 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © European Molecular Biology Organization 1986 Abstract In plants, glutamine synthetase (GS) is the enzyme primarily responsible for the assimilation of ammonia into organic nitrogen. In Phaseolus vulgaris a number of isoenzymic forms of GS are found, each of which consists of eight subunits of mol. wt 41 000‐45 000. The GS subunits of P. vulgaris have previously been shown to be encoded by a small multigene family and a partial cDNA clone for a nodule‐specific GS subunit has been obtained. We report here the isolation and nucleotide sequencing of two essentially full‐length GS cDNA clones (pR‐1 and pR‐2) from a root cDNA library and the deduced amino acid sequences of the corresponding GS subunits (355 amino acid residues each). The coding sequences of pR‐1 and pR‐2 are closely related (80% nucleotide homology, 88% amino acid homology), but their 5′‐ and 3′‐untranslated regions have diverged almost completely. Both pR‐1 and pR‐2 are related to, but distinct from, the nodule GS clone, pcPvNGS‐01 (or pN‐1). Hybridization to genomic Southern blots showed that the three GS mRNAs are encoded by three seperate genes and indicated the existence of a fourth class of GS gene. An S1 nuclease protection assay demonstrated the presence of R‐1 and R‐2 mRNA in both roots and leaves and confirmed that expression of the N‐1 gene is nodule‐specific. Expression of the R‐1 and R‐2 genes in the roots did not change significantly during nodulation. However, only the R‐1 gene is expressed in the nodules themselves, indicating that the R‐2 gene is specifically repressed during nodule development. glutamine synthetase (dpeaa)DE-He213 gene expression (dpeaa)DE-He213 cDNA sequence (dpeaa)DE-He213 amino acid sequence (dpeaa)DE-He213 Phaseolus vulgaris (dpeaa)DE-He213 Oliver, Jane E. verfasserin aut Forde, Brian G. verfasserin aut Saarelainen, Ritva verfasserin aut Miflin, Benjamin J. verfasserin aut Enthalten in The EMBO Journal Nature Publishing Group UK, 2023 5(1986), 7 vom: 01. Juli, Seite 1429-1435 (DE-627)266022529 (DE-600)1467419-1 1460-2075 nnns volume:5 year:1986 number:7 day:01 month:07 pages:1429-1435 https://dx.doi.org/10.1002/j.1460-2075.1986.tb04379.x X:SPRINGER Resolving-System lizenzpflichtig Volltext SYSFLAG_0 GBV_SPRINGER GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_72 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_252 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_2021 GBV_ILN_2050 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 5 1986 7 01 07 1429-1435
allfieldsGer	10.1002/j.1460-2075.1986.tb04379.x doi (DE-627)SPR057561168 (SPR)j.1460-2075.1986.tb04379.x-e DE-627 ger DE-627 rakwb eng Gebhardt, Christiane verfasserin aut Primary structure and differential expression of glutamine synthetase genes in nodules, roots and leaves of Phaseolus vulgaris 1986 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © European Molecular Biology Organization 1986 Abstract In plants, glutamine synthetase (GS) is the enzyme primarily responsible for the assimilation of ammonia into organic nitrogen. In Phaseolus vulgaris a number of isoenzymic forms of GS are found, each of which consists of eight subunits of mol. wt 41 000‐45 000. The GS subunits of P. vulgaris have previously been shown to be encoded by a small multigene family and a partial cDNA clone for a nodule‐specific GS subunit has been obtained. We report here the isolation and nucleotide sequencing of two essentially full‐length GS cDNA clones (pR‐1 and pR‐2) from a root cDNA library and the deduced amino acid sequences of the corresponding GS subunits (355 amino acid residues each). The coding sequences of pR‐1 and pR‐2 are closely related (80% nucleotide homology, 88% amino acid homology), but their 5′‐ and 3′‐untranslated regions have diverged almost completely. Both pR‐1 and pR‐2 are related to, but distinct from, the nodule GS clone, pcPvNGS‐01 (or pN‐1). Hybridization to genomic Southern blots showed that the three GS mRNAs are encoded by three seperate genes and indicated the existence of a fourth class of GS gene. An S1 nuclease protection assay demonstrated the presence of R‐1 and R‐2 mRNA in both roots and leaves and confirmed that expression of the N‐1 gene is nodule‐specific. Expression of the R‐1 and R‐2 genes in the roots did not change significantly during nodulation. However, only the R‐1 gene is expressed in the nodules themselves, indicating that the R‐2 gene is specifically repressed during nodule development. glutamine synthetase (dpeaa)DE-He213 gene expression (dpeaa)DE-He213 cDNA sequence (dpeaa)DE-He213 amino acid sequence (dpeaa)DE-He213 Phaseolus vulgaris (dpeaa)DE-He213 Oliver, Jane E. verfasserin aut Forde, Brian G. verfasserin aut Saarelainen, Ritva verfasserin aut Miflin, Benjamin J. verfasserin aut Enthalten in The EMBO Journal Nature Publishing Group UK, 2023 5(1986), 7 vom: 01. Juli, Seite 1429-1435 (DE-627)266022529 (DE-600)1467419-1 1460-2075 nnns volume:5 year:1986 number:7 day:01 month:07 pages:1429-1435 https://dx.doi.org/10.1002/j.1460-2075.1986.tb04379.x X:SPRINGER Resolving-System lizenzpflichtig Volltext SYSFLAG_0 GBV_SPRINGER GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_72 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_252 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_2021 GBV_ILN_2050 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 5 1986 7 01 07 1429-1435
allfieldsSound	10.1002/j.1460-2075.1986.tb04379.x doi (DE-627)SPR057561168 (SPR)j.1460-2075.1986.tb04379.x-e DE-627 ger DE-627 rakwb eng Gebhardt, Christiane verfasserin aut Primary structure and differential expression of glutamine synthetase genes in nodules, roots and leaves of Phaseolus vulgaris 1986 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © European Molecular Biology Organization 1986 Abstract In plants, glutamine synthetase (GS) is the enzyme primarily responsible for the assimilation of ammonia into organic nitrogen. In Phaseolus vulgaris a number of isoenzymic forms of GS are found, each of which consists of eight subunits of mol. wt 41 000‐45 000. The GS subunits of P. vulgaris have previously been shown to be encoded by a small multigene family and a partial cDNA clone for a nodule‐specific GS subunit has been obtained. We report here the isolation and nucleotide sequencing of two essentially full‐length GS cDNA clones (pR‐1 and pR‐2) from a root cDNA library and the deduced amino acid sequences of the corresponding GS subunits (355 amino acid residues each). The coding sequences of pR‐1 and pR‐2 are closely related (80% nucleotide homology, 88% amino acid homology), but their 5′‐ and 3′‐untranslated regions have diverged almost completely. Both pR‐1 and pR‐2 are related to, but distinct from, the nodule GS clone, pcPvNGS‐01 (or pN‐1). Hybridization to genomic Southern blots showed that the three GS mRNAs are encoded by three seperate genes and indicated the existence of a fourth class of GS gene. An S1 nuclease protection assay demonstrated the presence of R‐1 and R‐2 mRNA in both roots and leaves and confirmed that expression of the N‐1 gene is nodule‐specific. Expression of the R‐1 and R‐2 genes in the roots did not change significantly during nodulation. However, only the R‐1 gene is expressed in the nodules themselves, indicating that the R‐2 gene is specifically repressed during nodule development. glutamine synthetase (dpeaa)DE-He213 gene expression (dpeaa)DE-He213 cDNA sequence (dpeaa)DE-He213 amino acid sequence (dpeaa)DE-He213 Phaseolus vulgaris (dpeaa)DE-He213 Oliver, Jane E. verfasserin aut Forde, Brian G. verfasserin aut Saarelainen, Ritva verfasserin aut Miflin, Benjamin J. verfasserin aut Enthalten in The EMBO Journal Nature Publishing Group UK, 2023 5(1986), 7 vom: 01. Juli, Seite 1429-1435 (DE-627)266022529 (DE-600)1467419-1 1460-2075 nnns volume:5 year:1986 number:7 day:01 month:07 pages:1429-1435 https://dx.doi.org/10.1002/j.1460-2075.1986.tb04379.x X:SPRINGER Resolving-System lizenzpflichtig Volltext SYSFLAG_0 GBV_SPRINGER GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_72 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_252 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2014 GBV_ILN_2021 GBV_ILN_2050 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 5 1986 7 01 07 1429-1435
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author	Gebhardt, Christiane
spellingShingle	Gebhardt, Christiane misc glutamine synthetase misc gene expression misc cDNA sequence misc amino acid sequence misc Phaseolus vulgaris Primary structure and differential expression of glutamine synthetase genes in nodules, roots and leaves of Phaseolus vulgaris
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topic_title	Primary structure and differential expression of glutamine synthetase genes in nodules, roots and leaves of Phaseolus vulgaris glutamine synthetase (dpeaa)DE-He213 gene expression (dpeaa)DE-He213 cDNA sequence (dpeaa)DE-He213 amino acid sequence (dpeaa)DE-He213 Phaseolus vulgaris (dpeaa)DE-He213
topic	misc glutamine synthetase misc gene expression misc cDNA sequence misc amino acid sequence misc Phaseolus vulgaris
topic_unstemmed	misc glutamine synthetase misc gene expression misc cDNA sequence misc amino acid sequence misc Phaseolus vulgaris
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title	Primary structure and differential expression of glutamine synthetase genes in nodules, roots and leaves of Phaseolus vulgaris
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title_full	Primary structure and differential expression of glutamine synthetase genes in nodules, roots and leaves of Phaseolus vulgaris
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author_browse	Gebhardt, Christiane Oliver, Jane E. Forde, Brian G. Saarelainen, Ritva Miflin, Benjamin J.
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title_sort	primary structure and differential expression of glutamine synthetase genes in nodules, roots and leaves of phaseolus vulgaris
title_auth	Primary structure and differential expression of glutamine synthetase genes in nodules, roots and leaves of Phaseolus vulgaris
abstract	Abstract In plants, glutamine synthetase (GS) is the enzyme primarily responsible for the assimilation of ammonia into organic nitrogen. In Phaseolus vulgaris a number of isoenzymic forms of GS are found, each of which consists of eight subunits of mol. wt 41 000‐45 000. The GS subunits of P. vulgaris have previously been shown to be encoded by a small multigene family and a partial cDNA clone for a nodule‐specific GS subunit has been obtained. We report here the isolation and nucleotide sequencing of two essentially full‐length GS cDNA clones (pR‐1 and pR‐2) from a root cDNA library and the deduced amino acid sequences of the corresponding GS subunits (355 amino acid residues each). The coding sequences of pR‐1 and pR‐2 are closely related (80% nucleotide homology, 88% amino acid homology), but their 5′‐ and 3′‐untranslated regions have diverged almost completely. Both pR‐1 and pR‐2 are related to, but distinct from, the nodule GS clone, pcPvNGS‐01 (or pN‐1). Hybridization to genomic Southern blots showed that the three GS mRNAs are encoded by three seperate genes and indicated the existence of a fourth class of GS gene. An S1 nuclease protection assay demonstrated the presence of R‐1 and R‐2 mRNA in both roots and leaves and confirmed that expression of the N‐1 gene is nodule‐specific. Expression of the R‐1 and R‐2 genes in the roots did not change significantly during nodulation. However, only the R‐1 gene is expressed in the nodules themselves, indicating that the R‐2 gene is specifically repressed during nodule development. © European Molecular Biology Organization 1986
abstractGer	Abstract In plants, glutamine synthetase (GS) is the enzyme primarily responsible for the assimilation of ammonia into organic nitrogen. In Phaseolus vulgaris a number of isoenzymic forms of GS are found, each of which consists of eight subunits of mol. wt 41 000‐45 000. The GS subunits of P. vulgaris have previously been shown to be encoded by a small multigene family and a partial cDNA clone for a nodule‐specific GS subunit has been obtained. We report here the isolation and nucleotide sequencing of two essentially full‐length GS cDNA clones (pR‐1 and pR‐2) from a root cDNA library and the deduced amino acid sequences of the corresponding GS subunits (355 amino acid residues each). The coding sequences of pR‐1 and pR‐2 are closely related (80% nucleotide homology, 88% amino acid homology), but their 5′‐ and 3′‐untranslated regions have diverged almost completely. Both pR‐1 and pR‐2 are related to, but distinct from, the nodule GS clone, pcPvNGS‐01 (or pN‐1). Hybridization to genomic Southern blots showed that the three GS mRNAs are encoded by three seperate genes and indicated the existence of a fourth class of GS gene. An S1 nuclease protection assay demonstrated the presence of R‐1 and R‐2 mRNA in both roots and leaves and confirmed that expression of the N‐1 gene is nodule‐specific. Expression of the R‐1 and R‐2 genes in the roots did not change significantly during nodulation. However, only the R‐1 gene is expressed in the nodules themselves, indicating that the R‐2 gene is specifically repressed during nodule development. © European Molecular Biology Organization 1986
abstract_unstemmed	Abstract In plants, glutamine synthetase (GS) is the enzyme primarily responsible for the assimilation of ammonia into organic nitrogen. In Phaseolus vulgaris a number of isoenzymic forms of GS are found, each of which consists of eight subunits of mol. wt 41 000‐45 000. The GS subunits of P. vulgaris have previously been shown to be encoded by a small multigene family and a partial cDNA clone for a nodule‐specific GS subunit has been obtained. We report here the isolation and nucleotide sequencing of two essentially full‐length GS cDNA clones (pR‐1 and pR‐2) from a root cDNA library and the deduced amino acid sequences of the corresponding GS subunits (355 amino acid residues each). The coding sequences of pR‐1 and pR‐2 are closely related (80% nucleotide homology, 88% amino acid homology), but their 5′‐ and 3′‐untranslated regions have diverged almost completely. Both pR‐1 and pR‐2 are related to, but distinct from, the nodule GS clone, pcPvNGS‐01 (or pN‐1). Hybridization to genomic Southern blots showed that the three GS mRNAs are encoded by three seperate genes and indicated the existence of a fourth class of GS gene. An S1 nuclease protection assay demonstrated the presence of R‐1 and R‐2 mRNA in both roots and leaves and confirmed that expression of the N‐1 gene is nodule‐specific. Expression of the R‐1 and R‐2 genes in the roots did not change significantly during nodulation. However, only the R‐1 gene is expressed in the nodules themselves, indicating that the R‐2 gene is specifically repressed during nodule development. © European Molecular Biology Organization 1986
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title_short	Primary structure and differential expression of glutamine synthetase genes in nodules, roots and leaves of Phaseolus vulgaris
url	https://dx.doi.org/10.1002/j.1460-2075.1986.tb04379.x
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Primary structure and differential expression of glutamine synthetase genes in nodules, roots and leaves of Phaseolus vulgaris

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