Optimization of culture conditions for HBV-specific T cell expansion in vitro from chronically infected patients

Background Hepatitis B virus (HBV) clearance depends on an effective adaptive immune response, especially HBV-specific T cell-mediated cellular immunity; however, it is difficult to produce enough HBV-specific T cells effectively. Results In this work, we investigated the proportions of stimulated c...
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Autor*in:	Wang, Li [verfasserIn] Chen, Hongjiao [verfasserIn] Yang, Yuanqi [verfasserIn] Huang, Ying [verfasserIn] Chen, Weixian [verfasserIn] Mu, Di [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2024

Schlagwörter:	CD8 + T cells Expansion Hepatitis B virus

Anmerkung:	© The Author(s) 2024

Übergeordnetes Werk:	Enthalten in: BMC biotechnology - BioMed Central, 2001, 24(2024), 1 vom: 14. Okt.
Übergeordnetes Werk:	volume:24 ; year:2024 ; number:1 ; day:14 ; month:10

Links:	Volltext

DOI / URN:	10.1186/s12896-024-00908-8

Katalog-ID:	SPR057778426

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520			\|a Background Hepatitis B virus (HBV) clearance depends on an effective adaptive immune response, especially HBV-specific T cell-mediated cellular immunity; however, it is difficult to produce enough HBV-specific T cells effectively. Results In this work, we investigated the proportions of stimulated cells, serum, and culture media as the three primary factors to determine the most effective procedure and applied it to HLA-A2 (+) people. In parallel, we also examined the correlation between clinical parameters and HBV-specific immunity. Concerning amplification efficiency, 4 × $ 10^{5} $ cells stimulation was superior to 2 × $ 10^{6} $ cells stimulation, AIM-V medium outperformed 1640 medium, and fetal bovine serum (FBS) exceeded human AB serum under comparable conditions. As expected, this procedure is also suitable for developing HBV-specific CD8 + T cells in HLA-A2(+) individuals. Expanded HBV-specific T cell responses decreased with treatment time and were negatively correlated with HBV DNA and HBsAg. Furthermore, the number of HBV-specific IFN-γ + SFCs was strongly correlated with the ALT level and negatively correlated with the absolute lymphocyte count and the ALB concentration. Conclusions We confirm that stimulating 4 × $ 10^{5} $ PBMCs in AIM-V medium supplemented with 10% FBS is the best approach and that HBeAg, HBsAg, and ALB are independent predictors of HBV-specific T-cell responses.
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allfields	10.1186/s12896-024-00908-8 doi (DE-627)SPR057778426 (SPR)s12896-024-00908-8-e DE-627 ger DE-627 rakwb eng 570 610 VZ 12 ssgn BIODIV DE-30 fid 58.30 bkl Wang, Li verfasserin aut Optimization of culture conditions for HBV-specific T cell expansion in vitro from chronically infected patients 2024 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s) 2024 Background Hepatitis B virus (HBV) clearance depends on an effective adaptive immune response, especially HBV-specific T cell-mediated cellular immunity; however, it is difficult to produce enough HBV-specific T cells effectively. Results In this work, we investigated the proportions of stimulated cells, serum, and culture media as the three primary factors to determine the most effective procedure and applied it to HLA-A2 (+) people. In parallel, we also examined the correlation between clinical parameters and HBV-specific immunity. Concerning amplification efficiency, 4 × $ 10^{5} $ cells stimulation was superior to 2 × $ 10^{6} $ cells stimulation, AIM-V medium outperformed 1640 medium, and fetal bovine serum (FBS) exceeded human AB serum under comparable conditions. As expected, this procedure is also suitable for developing HBV-specific CD8 + T cells in HLA-A2(+) individuals. Expanded HBV-specific T cell responses decreased with treatment time and were negatively correlated with HBV DNA and HBsAg. Furthermore, the number of HBV-specific IFN-γ + SFCs was strongly correlated with the ALT level and negatively correlated with the absolute lymphocyte count and the ALB concentration. Conclusions We confirm that stimulating 4 × $ 10^{5} $ PBMCs in AIM-V medium supplemented with 10% FBS is the best approach and that HBeAg, HBsAg, and ALB are independent predictors of HBV-specific T-cell responses. CD8 + T cells (dpeaa)DE-He213 Expansion (dpeaa)DE-He213 Hepatitis B virus (dpeaa)DE-He213 Chen, Hongjiao verfasserin aut Yang, Yuanqi verfasserin aut Huang, Ying verfasserin aut Chen, Weixian verfasserin aut Mu, Di verfasserin aut Enthalten in BMC biotechnology BioMed Central, 2001 24(2024), 1 vom: 14. Okt. (DE-627)332164837 (DE-600)2052746-9 1472-6750 nnns volume:24 year:2024 number:1 day:14 month:10 https://dx.doi.org/10.1186/s12896-024-00908-8 X:SPRINGER Resolving-System kostenfrei Volltext SYSFLAG_0 GBV_SPRINGER FID-BIODIV SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_72 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2108 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2119 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4029 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4116 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4155 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 58.30 VZ AR 24 2024 1 14 10
spelling	10.1186/s12896-024-00908-8 doi (DE-627)SPR057778426 (SPR)s12896-024-00908-8-e DE-627 ger DE-627 rakwb eng 570 610 VZ 12 ssgn BIODIV DE-30 fid 58.30 bkl Wang, Li verfasserin aut Optimization of culture conditions for HBV-specific T cell expansion in vitro from chronically infected patients 2024 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s) 2024 Background Hepatitis B virus (HBV) clearance depends on an effective adaptive immune response, especially HBV-specific T cell-mediated cellular immunity; however, it is difficult to produce enough HBV-specific T cells effectively. Results In this work, we investigated the proportions of stimulated cells, serum, and culture media as the three primary factors to determine the most effective procedure and applied it to HLA-A2 (+) people. In parallel, we also examined the correlation between clinical parameters and HBV-specific immunity. Concerning amplification efficiency, 4 × $ 10^{5} $ cells stimulation was superior to 2 × $ 10^{6} $ cells stimulation, AIM-V medium outperformed 1640 medium, and fetal bovine serum (FBS) exceeded human AB serum under comparable conditions. As expected, this procedure is also suitable for developing HBV-specific CD8 + T cells in HLA-A2(+) individuals. Expanded HBV-specific T cell responses decreased with treatment time and were negatively correlated with HBV DNA and HBsAg. Furthermore, the number of HBV-specific IFN-γ + SFCs was strongly correlated with the ALT level and negatively correlated with the absolute lymphocyte count and the ALB concentration. Conclusions We confirm that stimulating 4 × $ 10^{5} $ PBMCs in AIM-V medium supplemented with 10% FBS is the best approach and that HBeAg, HBsAg, and ALB are independent predictors of HBV-specific T-cell responses. CD8 + T cells (dpeaa)DE-He213 Expansion (dpeaa)DE-He213 Hepatitis B virus (dpeaa)DE-He213 Chen, Hongjiao verfasserin aut Yang, Yuanqi verfasserin aut Huang, Ying verfasserin aut Chen, Weixian verfasserin aut Mu, Di verfasserin aut Enthalten in BMC biotechnology BioMed Central, 2001 24(2024), 1 vom: 14. Okt. (DE-627)332164837 (DE-600)2052746-9 1472-6750 nnns volume:24 year:2024 number:1 day:14 month:10 https://dx.doi.org/10.1186/s12896-024-00908-8 X:SPRINGER Resolving-System kostenfrei Volltext SYSFLAG_0 GBV_SPRINGER FID-BIODIV SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_72 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2108 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2119 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4029 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4116 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4155 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 58.30 VZ AR 24 2024 1 14 10
allfields_unstemmed	10.1186/s12896-024-00908-8 doi (DE-627)SPR057778426 (SPR)s12896-024-00908-8-e DE-627 ger DE-627 rakwb eng 570 610 VZ 12 ssgn BIODIV DE-30 fid 58.30 bkl Wang, Li verfasserin aut Optimization of culture conditions for HBV-specific T cell expansion in vitro from chronically infected patients 2024 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s) 2024 Background Hepatitis B virus (HBV) clearance depends on an effective adaptive immune response, especially HBV-specific T cell-mediated cellular immunity; however, it is difficult to produce enough HBV-specific T cells effectively. Results In this work, we investigated the proportions of stimulated cells, serum, and culture media as the three primary factors to determine the most effective procedure and applied it to HLA-A2 (+) people. In parallel, we also examined the correlation between clinical parameters and HBV-specific immunity. Concerning amplification efficiency, 4 × $ 10^{5} $ cells stimulation was superior to 2 × $ 10^{6} $ cells stimulation, AIM-V medium outperformed 1640 medium, and fetal bovine serum (FBS) exceeded human AB serum under comparable conditions. As expected, this procedure is also suitable for developing HBV-specific CD8 + T cells in HLA-A2(+) individuals. Expanded HBV-specific T cell responses decreased with treatment time and were negatively correlated with HBV DNA and HBsAg. Furthermore, the number of HBV-specific IFN-γ + SFCs was strongly correlated with the ALT level and negatively correlated with the absolute lymphocyte count and the ALB concentration. Conclusions We confirm that stimulating 4 × $ 10^{5} $ PBMCs in AIM-V medium supplemented with 10% FBS is the best approach and that HBeAg, HBsAg, and ALB are independent predictors of HBV-specific T-cell responses. CD8 + T cells (dpeaa)DE-He213 Expansion (dpeaa)DE-He213 Hepatitis B virus (dpeaa)DE-He213 Chen, Hongjiao verfasserin aut Yang, Yuanqi verfasserin aut Huang, Ying verfasserin aut Chen, Weixian verfasserin aut Mu, Di verfasserin aut Enthalten in BMC biotechnology BioMed Central, 2001 24(2024), 1 vom: 14. Okt. (DE-627)332164837 (DE-600)2052746-9 1472-6750 nnns volume:24 year:2024 number:1 day:14 month:10 https://dx.doi.org/10.1186/s12896-024-00908-8 X:SPRINGER Resolving-System kostenfrei Volltext SYSFLAG_0 GBV_SPRINGER FID-BIODIV SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_72 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2108 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2119 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4029 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4116 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4155 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 58.30 VZ AR 24 2024 1 14 10
allfieldsGer	10.1186/s12896-024-00908-8 doi (DE-627)SPR057778426 (SPR)s12896-024-00908-8-e DE-627 ger DE-627 rakwb eng 570 610 VZ 12 ssgn BIODIV DE-30 fid 58.30 bkl Wang, Li verfasserin aut Optimization of culture conditions for HBV-specific T cell expansion in vitro from chronically infected patients 2024 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s) 2024 Background Hepatitis B virus (HBV) clearance depends on an effective adaptive immune response, especially HBV-specific T cell-mediated cellular immunity; however, it is difficult to produce enough HBV-specific T cells effectively. Results In this work, we investigated the proportions of stimulated cells, serum, and culture media as the three primary factors to determine the most effective procedure and applied it to HLA-A2 (+) people. In parallel, we also examined the correlation between clinical parameters and HBV-specific immunity. Concerning amplification efficiency, 4 × $ 10^{5} $ cells stimulation was superior to 2 × $ 10^{6} $ cells stimulation, AIM-V medium outperformed 1640 medium, and fetal bovine serum (FBS) exceeded human AB serum under comparable conditions. As expected, this procedure is also suitable for developing HBV-specific CD8 + T cells in HLA-A2(+) individuals. Expanded HBV-specific T cell responses decreased with treatment time and were negatively correlated with HBV DNA and HBsAg. Furthermore, the number of HBV-specific IFN-γ + SFCs was strongly correlated with the ALT level and negatively correlated with the absolute lymphocyte count and the ALB concentration. Conclusions We confirm that stimulating 4 × $ 10^{5} $ PBMCs in AIM-V medium supplemented with 10% FBS is the best approach and that HBeAg, HBsAg, and ALB are independent predictors of HBV-specific T-cell responses. CD8 + T cells (dpeaa)DE-He213 Expansion (dpeaa)DE-He213 Hepatitis B virus (dpeaa)DE-He213 Chen, Hongjiao verfasserin aut Yang, Yuanqi verfasserin aut Huang, Ying verfasserin aut Chen, Weixian verfasserin aut Mu, Di verfasserin aut Enthalten in BMC biotechnology BioMed Central, 2001 24(2024), 1 vom: 14. Okt. (DE-627)332164837 (DE-600)2052746-9 1472-6750 nnns volume:24 year:2024 number:1 day:14 month:10 https://dx.doi.org/10.1186/s12896-024-00908-8 X:SPRINGER Resolving-System kostenfrei Volltext SYSFLAG_0 GBV_SPRINGER FID-BIODIV SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_72 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2108 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2119 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4029 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4116 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4155 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 58.30 VZ AR 24 2024 1 14 10
allfieldsSound	10.1186/s12896-024-00908-8 doi (DE-627)SPR057778426 (SPR)s12896-024-00908-8-e DE-627 ger DE-627 rakwb eng 570 610 VZ 12 ssgn BIODIV DE-30 fid 58.30 bkl Wang, Li verfasserin aut Optimization of culture conditions for HBV-specific T cell expansion in vitro from chronically infected patients 2024 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s) 2024 Background Hepatitis B virus (HBV) clearance depends on an effective adaptive immune response, especially HBV-specific T cell-mediated cellular immunity; however, it is difficult to produce enough HBV-specific T cells effectively. Results In this work, we investigated the proportions of stimulated cells, serum, and culture media as the three primary factors to determine the most effective procedure and applied it to HLA-A2 (+) people. In parallel, we also examined the correlation between clinical parameters and HBV-specific immunity. Concerning amplification efficiency, 4 × $ 10^{5} $ cells stimulation was superior to 2 × $ 10^{6} $ cells stimulation, AIM-V medium outperformed 1640 medium, and fetal bovine serum (FBS) exceeded human AB serum under comparable conditions. As expected, this procedure is also suitable for developing HBV-specific CD8 + T cells in HLA-A2(+) individuals. Expanded HBV-specific T cell responses decreased with treatment time and were negatively correlated with HBV DNA and HBsAg. Furthermore, the number of HBV-specific IFN-γ + SFCs was strongly correlated with the ALT level and negatively correlated with the absolute lymphocyte count and the ALB concentration. Conclusions We confirm that stimulating 4 × $ 10^{5} $ PBMCs in AIM-V medium supplemented with 10% FBS is the best approach and that HBeAg, HBsAg, and ALB are independent predictors of HBV-specific T-cell responses. CD8 + T cells (dpeaa)DE-He213 Expansion (dpeaa)DE-He213 Hepatitis B virus (dpeaa)DE-He213 Chen, Hongjiao verfasserin aut Yang, Yuanqi verfasserin aut Huang, Ying verfasserin aut Chen, Weixian verfasserin aut Mu, Di verfasserin aut Enthalten in BMC biotechnology BioMed Central, 2001 24(2024), 1 vom: 14. Okt. (DE-627)332164837 (DE-600)2052746-9 1472-6750 nnns volume:24 year:2024 number:1 day:14 month:10 https://dx.doi.org/10.1186/s12896-024-00908-8 X:SPRINGER Resolving-System kostenfrei Volltext SYSFLAG_0 GBV_SPRINGER FID-BIODIV SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_72 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2025 GBV_ILN_2031 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2061 GBV_ILN_2108 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2119 GBV_ILN_2190 GBV_ILN_4012 GBV_ILN_4029 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4116 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4155 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 58.30 VZ AR 24 2024 1 14 10
language	English
source	Enthalten in BMC biotechnology 24(2024), 1 vom: 14. Okt. volume:24 year:2024 number:1 day:14 month:10
sourceStr	Enthalten in BMC biotechnology 24(2024), 1 vom: 14. Okt. volume:24 year:2024 number:1 day:14 month:10
format_phy_str_mv	Article
institution	findex.gbv.de
topic_facet	CD8 + T cells Expansion Hepatitis B virus
dewey-raw	570
isfreeaccess_bool	true
container_title	BMC biotechnology
authorswithroles_txt_mv	Wang, Li @@aut@@ Chen, Hongjiao @@aut@@ Yang, Yuanqi @@aut@@ Huang, Ying @@aut@@ Chen, Weixian @@aut@@ Mu, Di @@aut@@
publishDateDaySort_date	2024-10-14T00:00:00Z
hierarchy_top_id	332164837
dewey-sort	3570
id	SPR057778426
language_de	englisch
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author	Wang, Li
spellingShingle	Wang, Li ddc 570 ssgn 12 fid BIODIV bkl 58.30 misc CD8 + T cells misc Expansion misc Hepatitis B virus Optimization of culture conditions for HBV-specific T cell expansion in vitro from chronically infected patients
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topic_title	570 610 VZ 12 ssgn BIODIV DE-30 fid 58.30 bkl Optimization of culture conditions for HBV-specific T cell expansion in vitro from chronically infected patients CD8 + T cells (dpeaa)DE-He213 Expansion (dpeaa)DE-He213 Hepatitis B virus (dpeaa)DE-He213
topic	ddc 570 ssgn 12 fid BIODIV bkl 58.30 misc CD8 + T cells misc Expansion misc Hepatitis B virus
topic_unstemmed	ddc 570 ssgn 12 fid BIODIV bkl 58.30 misc CD8 + T cells misc Expansion misc Hepatitis B virus
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title	Optimization of culture conditions for HBV-specific T cell expansion in vitro from chronically infected patients
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title_full	Optimization of culture conditions for HBV-specific T cell expansion in vitro from chronically infected patients
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author_browse	Wang, Li Chen, Hongjiao Yang, Yuanqi Huang, Ying Chen, Weixian Mu, Di
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title_sort	optimization of culture conditions for hbv-specific t cell expansion in vitro from chronically infected patients
title_auth	Optimization of culture conditions for HBV-specific T cell expansion in vitro from chronically infected patients
abstract	Background Hepatitis B virus (HBV) clearance depends on an effective adaptive immune response, especially HBV-specific T cell-mediated cellular immunity; however, it is difficult to produce enough HBV-specific T cells effectively. Results In this work, we investigated the proportions of stimulated cells, serum, and culture media as the three primary factors to determine the most effective procedure and applied it to HLA-A2 (+) people. In parallel, we also examined the correlation between clinical parameters and HBV-specific immunity. Concerning amplification efficiency, 4 × $ 10^{5} $ cells stimulation was superior to 2 × $ 10^{6} $ cells stimulation, AIM-V medium outperformed 1640 medium, and fetal bovine serum (FBS) exceeded human AB serum under comparable conditions. As expected, this procedure is also suitable for developing HBV-specific CD8 + T cells in HLA-A2(+) individuals. Expanded HBV-specific T cell responses decreased with treatment time and were negatively correlated with HBV DNA and HBsAg. Furthermore, the number of HBV-specific IFN-γ + SFCs was strongly correlated with the ALT level and negatively correlated with the absolute lymphocyte count and the ALB concentration. Conclusions We confirm that stimulating 4 × $ 10^{5} $ PBMCs in AIM-V medium supplemented with 10% FBS is the best approach and that HBeAg, HBsAg, and ALB are independent predictors of HBV-specific T-cell responses. © The Author(s) 2024
abstractGer	Background Hepatitis B virus (HBV) clearance depends on an effective adaptive immune response, especially HBV-specific T cell-mediated cellular immunity; however, it is difficult to produce enough HBV-specific T cells effectively. Results In this work, we investigated the proportions of stimulated cells, serum, and culture media as the three primary factors to determine the most effective procedure and applied it to HLA-A2 (+) people. In parallel, we also examined the correlation between clinical parameters and HBV-specific immunity. Concerning amplification efficiency, 4 × $ 10^{5} $ cells stimulation was superior to 2 × $ 10^{6} $ cells stimulation, AIM-V medium outperformed 1640 medium, and fetal bovine serum (FBS) exceeded human AB serum under comparable conditions. As expected, this procedure is also suitable for developing HBV-specific CD8 + T cells in HLA-A2(+) individuals. Expanded HBV-specific T cell responses decreased with treatment time and were negatively correlated with HBV DNA and HBsAg. Furthermore, the number of HBV-specific IFN-γ + SFCs was strongly correlated with the ALT level and negatively correlated with the absolute lymphocyte count and the ALB concentration. Conclusions We confirm that stimulating 4 × $ 10^{5} $ PBMCs in AIM-V medium supplemented with 10% FBS is the best approach and that HBeAg, HBsAg, and ALB are independent predictors of HBV-specific T-cell responses. © The Author(s) 2024
abstract_unstemmed	Background Hepatitis B virus (HBV) clearance depends on an effective adaptive immune response, especially HBV-specific T cell-mediated cellular immunity; however, it is difficult to produce enough HBV-specific T cells effectively. Results In this work, we investigated the proportions of stimulated cells, serum, and culture media as the three primary factors to determine the most effective procedure and applied it to HLA-A2 (+) people. In parallel, we also examined the correlation between clinical parameters and HBV-specific immunity. Concerning amplification efficiency, 4 × $ 10^{5} $ cells stimulation was superior to 2 × $ 10^{6} $ cells stimulation, AIM-V medium outperformed 1640 medium, and fetal bovine serum (FBS) exceeded human AB serum under comparable conditions. As expected, this procedure is also suitable for developing HBV-specific CD8 + T cells in HLA-A2(+) individuals. Expanded HBV-specific T cell responses decreased with treatment time and were negatively correlated with HBV DNA and HBsAg. Furthermore, the number of HBV-specific IFN-γ + SFCs was strongly correlated with the ALT level and negatively correlated with the absolute lymphocyte count and the ALB concentration. Conclusions We confirm that stimulating 4 × $ 10^{5} $ PBMCs in AIM-V medium supplemented with 10% FBS is the best approach and that HBeAg, HBsAg, and ALB are independent predictors of HBV-specific T-cell responses. © The Author(s) 2024
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title_short	Optimization of culture conditions for HBV-specific T cell expansion in vitro from chronically infected patients
url	https://dx.doi.org/10.1186/s12896-024-00908-8
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author2	Chen, Hongjiao Yang, Yuanqi Huang, Ying Chen, Weixian Mu, Di
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Optimization of culture conditions for HBV-specific T cell expansion in vitro from chronically infected patients

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