TGF‐β cooperates with TGF‐α to induce the self–renewal of normal erythrocytic progenitors: evidence for an autocrine mechanism

Abstract Simultaneous addition of both TGF‐α and TGF‐β induces the sustained, long‐term outgrowth of chicken erythrocytic progenitor cells, referred to as T2ECs from both chick bone marrow and 2‐day‐old chicken embryos. By analysis for differentiation antigens and gene expression, these cells were s...
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Autor*in:	Gandrillon, Olivier [verfasserIn] Schmidt, Uwe [verfasserIn] Beug, Hartmut [verfasserIn] Samarut, Jacques [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	1999

Schlagwörter:	erythropoiesis progenitors self‐renewal transforming growth factors

Anmerkung:	© European Molecular Biology Organization 1999

Übergeordnetes Werk:	Enthalten in: The EMBO Journal - Nature Publishing Group UK, 2023, 18(1999), 10 vom: 17. Mai, Seite 2764-2781
Übergeordnetes Werk:	volume:18 ; year:1999 ; number:10 ; day:17 ; month:05 ; pages:2764-2781

Links:	Volltext

DOI / URN:	10.1093/emboj/18.10.2764

Katalog-ID:	SPR057808503

Internformat


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520			\|a Abstract Simultaneous addition of both TGF‐α and TGF‐β induces the sustained, long‐term outgrowth of chicken erythrocytic progenitor cells, referred to as T2ECs from both chick bone marrow and 2‐day‐old chicken embryos. By analysis for differentiation antigens and gene expression, these cells were shown to represent very immature haematopoietic progenitors committed to the erythrocytic lineage. T2ECs differentiate into almost pure populations of fully mature erythrocytes within 6 days, when TGF‐α and TGF‐β are withdrawn and the cells exposed to anaemic chicken serum plus insulin. Outgrowth of these cells from various sources invariably required both TGF‐α and TGF‐β, as well as glucocorticoids. Proliferating, established T2ECs still require TGF‐α, but are independent of exogenous TGF‐β. Using a TGF‐β‐neutralizing antibody or expressing a dominant‐negative TGF‐β receptor II, we demonstrate that T2ECs generate an autocrine loop involving TGF‐β during their establishment, which is required for sustained proliferation. Using specific inhibitors, we also show that signalling via Mek‐1 is specifically required for induction and maintenance of cell proliferation driven by cooperation between the TGF‐α and ‐β receptors. These results establish a novel mechanism by which self‐renewal of erythrocytic progenitors is induced and establish avian T2ECs as a new, quasi‐optimal model system to study erythrocytic progenitors.
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Indexfelder

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matchkey_str	article:14602075:1999----::gcoeaewttfonuehslrnwlfomlrtrctcrgntreie
hierarchy_sort_str	1999
publishDate	1999
allfields	10.1093/emboj/18.10.2764 doi (DE-627)SPR057808503 (SPR)18.10.2764-e DE-627 ger DE-627 rakwb eng Gandrillon, Olivier verfasserin aut TGF‐β cooperates with TGF‐α to induce the self–renewal of normal erythrocytic progenitors: evidence for an autocrine mechanism 1999 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © European Molecular Biology Organization 1999 Abstract Simultaneous addition of both TGF‐α and TGF‐β induces the sustained, long‐term outgrowth of chicken erythrocytic progenitor cells, referred to as T2ECs from both chick bone marrow and 2‐day‐old chicken embryos. By analysis for differentiation antigens and gene expression, these cells were shown to represent very immature haematopoietic progenitors committed to the erythrocytic lineage. T2ECs differentiate into almost pure populations of fully mature erythrocytes within 6 days, when TGF‐α and TGF‐β are withdrawn and the cells exposed to anaemic chicken serum plus insulin. Outgrowth of these cells from various sources invariably required both TGF‐α and TGF‐β, as well as glucocorticoids. Proliferating, established T2ECs still require TGF‐α, but are independent of exogenous TGF‐β. Using a TGF‐β‐neutralizing antibody or expressing a dominant‐negative TGF‐β receptor II, we demonstrate that T2ECs generate an autocrine loop involving TGF‐β during their establishment, which is required for sustained proliferation. Using specific inhibitors, we also show that signalling via Mek‐1 is specifically required for induction and maintenance of cell proliferation driven by cooperation between the TGF‐α and ‐β receptors. These results establish a novel mechanism by which self‐renewal of erythrocytic progenitors is induced and establish avian T2ECs as a new, quasi‐optimal model system to study erythrocytic progenitors. erythropoiesis (dpeaa)DE-He213 progenitors (dpeaa)DE-He213 self‐renewal (dpeaa)DE-He213 transforming growth factors (dpeaa)DE-He213 Schmidt, Uwe verfasserin aut Beug, Hartmut verfasserin aut Samarut, Jacques verfasserin aut Enthalten in The EMBO Journal Nature Publishing Group UK, 2023 18(1999), 10 vom: 17. Mai, Seite 2764-2781 (DE-627)266022529 (DE-600)1467419-1 1460-2075 nnns volume:18 year:1999 number:10 day:17 month:05 pages:2764-2781 https://dx.doi.org/10.1093/emboj/18.10.2764 X:SPRINGER Resolving-System lizenzpflichtig Volltext SYSFLAG_0 GBV_SPRINGER GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_72 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_161 GBV_ILN_168 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_252 GBV_ILN_266 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2018 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4029 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4155 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4393 GBV_ILN_4700 AR 18 1999 10 17 05 2764-2781
spelling	10.1093/emboj/18.10.2764 doi (DE-627)SPR057808503 (SPR)18.10.2764-e DE-627 ger DE-627 rakwb eng Gandrillon, Olivier verfasserin aut TGF‐β cooperates with TGF‐α to induce the self–renewal of normal erythrocytic progenitors: evidence for an autocrine mechanism 1999 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © European Molecular Biology Organization 1999 Abstract Simultaneous addition of both TGF‐α and TGF‐β induces the sustained, long‐term outgrowth of chicken erythrocytic progenitor cells, referred to as T2ECs from both chick bone marrow and 2‐day‐old chicken embryos. By analysis for differentiation antigens and gene expression, these cells were shown to represent very immature haematopoietic progenitors committed to the erythrocytic lineage. T2ECs differentiate into almost pure populations of fully mature erythrocytes within 6 days, when TGF‐α and TGF‐β are withdrawn and the cells exposed to anaemic chicken serum plus insulin. Outgrowth of these cells from various sources invariably required both TGF‐α and TGF‐β, as well as glucocorticoids. Proliferating, established T2ECs still require TGF‐α, but are independent of exogenous TGF‐β. Using a TGF‐β‐neutralizing antibody or expressing a dominant‐negative TGF‐β receptor II, we demonstrate that T2ECs generate an autocrine loop involving TGF‐β during their establishment, which is required for sustained proliferation. Using specific inhibitors, we also show that signalling via Mek‐1 is specifically required for induction and maintenance of cell proliferation driven by cooperation between the TGF‐α and ‐β receptors. These results establish a novel mechanism by which self‐renewal of erythrocytic progenitors is induced and establish avian T2ECs as a new, quasi‐optimal model system to study erythrocytic progenitors. erythropoiesis (dpeaa)DE-He213 progenitors (dpeaa)DE-He213 self‐renewal (dpeaa)DE-He213 transforming growth factors (dpeaa)DE-He213 Schmidt, Uwe verfasserin aut Beug, Hartmut verfasserin aut Samarut, Jacques verfasserin aut Enthalten in The EMBO Journal Nature Publishing Group UK, 2023 18(1999), 10 vom: 17. Mai, Seite 2764-2781 (DE-627)266022529 (DE-600)1467419-1 1460-2075 nnns volume:18 year:1999 number:10 day:17 month:05 pages:2764-2781 https://dx.doi.org/10.1093/emboj/18.10.2764 X:SPRINGER Resolving-System lizenzpflichtig Volltext SYSFLAG_0 GBV_SPRINGER GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_72 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_161 GBV_ILN_168 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_252 GBV_ILN_266 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2018 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4029 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4155 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4393 GBV_ILN_4700 AR 18 1999 10 17 05 2764-2781
allfields_unstemmed	10.1093/emboj/18.10.2764 doi (DE-627)SPR057808503 (SPR)18.10.2764-e DE-627 ger DE-627 rakwb eng Gandrillon, Olivier verfasserin aut TGF‐β cooperates with TGF‐α to induce the self–renewal of normal erythrocytic progenitors: evidence for an autocrine mechanism 1999 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © European Molecular Biology Organization 1999 Abstract Simultaneous addition of both TGF‐α and TGF‐β induces the sustained, long‐term outgrowth of chicken erythrocytic progenitor cells, referred to as T2ECs from both chick bone marrow and 2‐day‐old chicken embryos. By analysis for differentiation antigens and gene expression, these cells were shown to represent very immature haematopoietic progenitors committed to the erythrocytic lineage. T2ECs differentiate into almost pure populations of fully mature erythrocytes within 6 days, when TGF‐α and TGF‐β are withdrawn and the cells exposed to anaemic chicken serum plus insulin. Outgrowth of these cells from various sources invariably required both TGF‐α and TGF‐β, as well as glucocorticoids. Proliferating, established T2ECs still require TGF‐α, but are independent of exogenous TGF‐β. Using a TGF‐β‐neutralizing antibody or expressing a dominant‐negative TGF‐β receptor II, we demonstrate that T2ECs generate an autocrine loop involving TGF‐β during their establishment, which is required for sustained proliferation. Using specific inhibitors, we also show that signalling via Mek‐1 is specifically required for induction and maintenance of cell proliferation driven by cooperation between the TGF‐α and ‐β receptors. These results establish a novel mechanism by which self‐renewal of erythrocytic progenitors is induced and establish avian T2ECs as a new, quasi‐optimal model system to study erythrocytic progenitors. erythropoiesis (dpeaa)DE-He213 progenitors (dpeaa)DE-He213 self‐renewal (dpeaa)DE-He213 transforming growth factors (dpeaa)DE-He213 Schmidt, Uwe verfasserin aut Beug, Hartmut verfasserin aut Samarut, Jacques verfasserin aut Enthalten in The EMBO Journal Nature Publishing Group UK, 2023 18(1999), 10 vom: 17. Mai, Seite 2764-2781 (DE-627)266022529 (DE-600)1467419-1 1460-2075 nnns volume:18 year:1999 number:10 day:17 month:05 pages:2764-2781 https://dx.doi.org/10.1093/emboj/18.10.2764 X:SPRINGER Resolving-System lizenzpflichtig Volltext SYSFLAG_0 GBV_SPRINGER GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_72 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_161 GBV_ILN_168 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_252 GBV_ILN_266 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2018 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4029 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4155 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4393 GBV_ILN_4700 AR 18 1999 10 17 05 2764-2781
allfieldsGer	10.1093/emboj/18.10.2764 doi (DE-627)SPR057808503 (SPR)18.10.2764-e DE-627 ger DE-627 rakwb eng Gandrillon, Olivier verfasserin aut TGF‐β cooperates with TGF‐α to induce the self–renewal of normal erythrocytic progenitors: evidence for an autocrine mechanism 1999 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © European Molecular Biology Organization 1999 Abstract Simultaneous addition of both TGF‐α and TGF‐β induces the sustained, long‐term outgrowth of chicken erythrocytic progenitor cells, referred to as T2ECs from both chick bone marrow and 2‐day‐old chicken embryos. By analysis for differentiation antigens and gene expression, these cells were shown to represent very immature haematopoietic progenitors committed to the erythrocytic lineage. T2ECs differentiate into almost pure populations of fully mature erythrocytes within 6 days, when TGF‐α and TGF‐β are withdrawn and the cells exposed to anaemic chicken serum plus insulin. Outgrowth of these cells from various sources invariably required both TGF‐α and TGF‐β, as well as glucocorticoids. Proliferating, established T2ECs still require TGF‐α, but are independent of exogenous TGF‐β. Using a TGF‐β‐neutralizing antibody or expressing a dominant‐negative TGF‐β receptor II, we demonstrate that T2ECs generate an autocrine loop involving TGF‐β during their establishment, which is required for sustained proliferation. Using specific inhibitors, we also show that signalling via Mek‐1 is specifically required for induction and maintenance of cell proliferation driven by cooperation between the TGF‐α and ‐β receptors. These results establish a novel mechanism by which self‐renewal of erythrocytic progenitors is induced and establish avian T2ECs as a new, quasi‐optimal model system to study erythrocytic progenitors. erythropoiesis (dpeaa)DE-He213 progenitors (dpeaa)DE-He213 self‐renewal (dpeaa)DE-He213 transforming growth factors (dpeaa)DE-He213 Schmidt, Uwe verfasserin aut Beug, Hartmut verfasserin aut Samarut, Jacques verfasserin aut Enthalten in The EMBO Journal Nature Publishing Group UK, 2023 18(1999), 10 vom: 17. Mai, Seite 2764-2781 (DE-627)266022529 (DE-600)1467419-1 1460-2075 nnns volume:18 year:1999 number:10 day:17 month:05 pages:2764-2781 https://dx.doi.org/10.1093/emboj/18.10.2764 X:SPRINGER Resolving-System lizenzpflichtig Volltext SYSFLAG_0 GBV_SPRINGER GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_72 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_161 GBV_ILN_168 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_252 GBV_ILN_266 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2018 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4029 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4155 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4393 GBV_ILN_4700 AR 18 1999 10 17 05 2764-2781
allfieldsSound	10.1093/emboj/18.10.2764 doi (DE-627)SPR057808503 (SPR)18.10.2764-e DE-627 ger DE-627 rakwb eng Gandrillon, Olivier verfasserin aut TGF‐β cooperates with TGF‐α to induce the self–renewal of normal erythrocytic progenitors: evidence for an autocrine mechanism 1999 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © European Molecular Biology Organization 1999 Abstract Simultaneous addition of both TGF‐α and TGF‐β induces the sustained, long‐term outgrowth of chicken erythrocytic progenitor cells, referred to as T2ECs from both chick bone marrow and 2‐day‐old chicken embryos. By analysis for differentiation antigens and gene expression, these cells were shown to represent very immature haematopoietic progenitors committed to the erythrocytic lineage. T2ECs differentiate into almost pure populations of fully mature erythrocytes within 6 days, when TGF‐α and TGF‐β are withdrawn and the cells exposed to anaemic chicken serum plus insulin. Outgrowth of these cells from various sources invariably required both TGF‐α and TGF‐β, as well as glucocorticoids. Proliferating, established T2ECs still require TGF‐α, but are independent of exogenous TGF‐β. Using a TGF‐β‐neutralizing antibody or expressing a dominant‐negative TGF‐β receptor II, we demonstrate that T2ECs generate an autocrine loop involving TGF‐β during their establishment, which is required for sustained proliferation. Using specific inhibitors, we also show that signalling via Mek‐1 is specifically required for induction and maintenance of cell proliferation driven by cooperation between the TGF‐α and ‐β receptors. These results establish a novel mechanism by which self‐renewal of erythrocytic progenitors is induced and establish avian T2ECs as a new, quasi‐optimal model system to study erythrocytic progenitors. erythropoiesis (dpeaa)DE-He213 progenitors (dpeaa)DE-He213 self‐renewal (dpeaa)DE-He213 transforming growth factors (dpeaa)DE-He213 Schmidt, Uwe verfasserin aut Beug, Hartmut verfasserin aut Samarut, Jacques verfasserin aut Enthalten in The EMBO Journal Nature Publishing Group UK, 2023 18(1999), 10 vom: 17. Mai, Seite 2764-2781 (DE-627)266022529 (DE-600)1467419-1 1460-2075 nnns volume:18 year:1999 number:10 day:17 month:05 pages:2764-2781 https://dx.doi.org/10.1093/emboj/18.10.2764 X:SPRINGER Resolving-System lizenzpflichtig Volltext SYSFLAG_0 GBV_SPRINGER GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_72 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_161 GBV_ILN_168 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_252 GBV_ILN_266 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2018 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4029 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4155 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4393 GBV_ILN_4700 AR 18 1999 10 17 05 2764-2781
language	English
source	Enthalten in The EMBO Journal 18(1999), 10 vom: 17. Mai, Seite 2764-2781 volume:18 year:1999 number:10 day:17 month:05 pages:2764-2781
sourceStr	Enthalten in The EMBO Journal 18(1999), 10 vom: 17. Mai, Seite 2764-2781 volume:18 year:1999 number:10 day:17 month:05 pages:2764-2781
format_phy_str_mv	Article
institution	findex.gbv.de
topic_facet	erythropoiesis progenitors self‐renewal transforming growth factors
isfreeaccess_bool	false
container_title	The EMBO Journal
authorswithroles_txt_mv	Gandrillon, Olivier @@aut@@ Schmidt, Uwe @@aut@@ Beug, Hartmut @@aut@@ Samarut, Jacques @@aut@@
publishDateDaySort_date	1999-05-17T00:00:00Z
hierarchy_top_id	266022529
id	SPR057808503
language_de	englisch
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author	Gandrillon, Olivier
spellingShingle	Gandrillon, Olivier misc erythropoiesis misc progenitors misc self‐renewal misc transforming growth factors TGF‐β cooperates with TGF‐α to induce the self–renewal of normal erythrocytic progenitors: evidence for an autocrine mechanism
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topic_title	TGF‐β cooperates with TGF‐α to induce the self–renewal of normal erythrocytic progenitors: evidence for an autocrine mechanism erythropoiesis (dpeaa)DE-He213 progenitors (dpeaa)DE-He213 self‐renewal (dpeaa)DE-He213 transforming growth factors (dpeaa)DE-He213
topic	misc erythropoiesis misc progenitors misc self‐renewal misc transforming growth factors
topic_unstemmed	misc erythropoiesis misc progenitors misc self‐renewal misc transforming growth factors
topic_browse	misc erythropoiesis misc progenitors misc self‐renewal misc transforming growth factors
format_facet	Elektronische Aufsätze Aufsätze Elektronische Ressource
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title	TGF‐β cooperates with TGF‐α to induce the self–renewal of normal erythrocytic progenitors: evidence for an autocrine mechanism
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title_full	TGF‐β cooperates with TGF‐α to induce the self–renewal of normal erythrocytic progenitors: evidence for an autocrine mechanism
author_sort	Gandrillon, Olivier
journal	The EMBO Journal
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container_start_page	2764
author_browse	Gandrillon, Olivier Schmidt, Uwe Beug, Hartmut Samarut, Jacques
container_volume	18
format_se	Elektronische Aufsätze
author-letter	Gandrillon, Olivier
doi_str_mv	10.1093/emboj/18.10.2764
author2-role	verfasserin
title_sort	tgf‐β cooperates with tgf‐α to induce the self–renewal of normal erythrocytic progenitors: evidence for an autocrine mechanism
title_auth	TGF‐β cooperates with TGF‐α to induce the self–renewal of normal erythrocytic progenitors: evidence for an autocrine mechanism
abstract	Abstract Simultaneous addition of both TGF‐α and TGF‐β induces the sustained, long‐term outgrowth of chicken erythrocytic progenitor cells, referred to as T2ECs from both chick bone marrow and 2‐day‐old chicken embryos. By analysis for differentiation antigens and gene expression, these cells were shown to represent very immature haematopoietic progenitors committed to the erythrocytic lineage. T2ECs differentiate into almost pure populations of fully mature erythrocytes within 6 days, when TGF‐α and TGF‐β are withdrawn and the cells exposed to anaemic chicken serum plus insulin. Outgrowth of these cells from various sources invariably required both TGF‐α and TGF‐β, as well as glucocorticoids. Proliferating, established T2ECs still require TGF‐α, but are independent of exogenous TGF‐β. Using a TGF‐β‐neutralizing antibody or expressing a dominant‐negative TGF‐β receptor II, we demonstrate that T2ECs generate an autocrine loop involving TGF‐β during their establishment, which is required for sustained proliferation. Using specific inhibitors, we also show that signalling via Mek‐1 is specifically required for induction and maintenance of cell proliferation driven by cooperation between the TGF‐α and ‐β receptors. These results establish a novel mechanism by which self‐renewal of erythrocytic progenitors is induced and establish avian T2ECs as a new, quasi‐optimal model system to study erythrocytic progenitors. © European Molecular Biology Organization 1999
abstractGer	Abstract Simultaneous addition of both TGF‐α and TGF‐β induces the sustained, long‐term outgrowth of chicken erythrocytic progenitor cells, referred to as T2ECs from both chick bone marrow and 2‐day‐old chicken embryos. By analysis for differentiation antigens and gene expression, these cells were shown to represent very immature haematopoietic progenitors committed to the erythrocytic lineage. T2ECs differentiate into almost pure populations of fully mature erythrocytes within 6 days, when TGF‐α and TGF‐β are withdrawn and the cells exposed to anaemic chicken serum plus insulin. Outgrowth of these cells from various sources invariably required both TGF‐α and TGF‐β, as well as glucocorticoids. Proliferating, established T2ECs still require TGF‐α, but are independent of exogenous TGF‐β. Using a TGF‐β‐neutralizing antibody or expressing a dominant‐negative TGF‐β receptor II, we demonstrate that T2ECs generate an autocrine loop involving TGF‐β during their establishment, which is required for sustained proliferation. Using specific inhibitors, we also show that signalling via Mek‐1 is specifically required for induction and maintenance of cell proliferation driven by cooperation between the TGF‐α and ‐β receptors. These results establish a novel mechanism by which self‐renewal of erythrocytic progenitors is induced and establish avian T2ECs as a new, quasi‐optimal model system to study erythrocytic progenitors. © European Molecular Biology Organization 1999
abstract_unstemmed	Abstract Simultaneous addition of both TGF‐α and TGF‐β induces the sustained, long‐term outgrowth of chicken erythrocytic progenitor cells, referred to as T2ECs from both chick bone marrow and 2‐day‐old chicken embryos. By analysis for differentiation antigens and gene expression, these cells were shown to represent very immature haematopoietic progenitors committed to the erythrocytic lineage. T2ECs differentiate into almost pure populations of fully mature erythrocytes within 6 days, when TGF‐α and TGF‐β are withdrawn and the cells exposed to anaemic chicken serum plus insulin. Outgrowth of these cells from various sources invariably required both TGF‐α and TGF‐β, as well as glucocorticoids. Proliferating, established T2ECs still require TGF‐α, but are independent of exogenous TGF‐β. Using a TGF‐β‐neutralizing antibody or expressing a dominant‐negative TGF‐β receptor II, we demonstrate that T2ECs generate an autocrine loop involving TGF‐β during their establishment, which is required for sustained proliferation. Using specific inhibitors, we also show that signalling via Mek‐1 is specifically required for induction and maintenance of cell proliferation driven by cooperation between the TGF‐α and ‐β receptors. These results establish a novel mechanism by which self‐renewal of erythrocytic progenitors is induced and establish avian T2ECs as a new, quasi‐optimal model system to study erythrocytic progenitors. © European Molecular Biology Organization 1999
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title_short	TGF‐β cooperates with TGF‐α to induce the self–renewal of normal erythrocytic progenitors: evidence for an autocrine mechanism
url	https://dx.doi.org/10.1093/emboj/18.10.2764
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up_date	2024-10-16T04:50:52.859Z
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TGF‐β cooperates with TGF‐α to induce the self–renewal of normal erythrocytic progenitors: evidence for an autocrine mechanism

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