Regulatory cross‐talk between adhesin operons in Escherichia coli: inhibition of type 1 fimbriae expression by the PapB protein
Abstract Pathogenic Escherichia coli often carry determinants for several different adhesins. We show a direct communication between two adhesin gene clusters in uropathogenic E.coli: type 1 fimbriae (fim) and pyelonephritis‐associated pili (pap). A regulator of pap, PapB, is a key factor in this cr...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
Xia, Yan [verfasserIn] Gally, David [verfasserIn] Forsman‐Semb, Kristina [verfasserIn] Uhlin, Bernt Eric [verfasserIn] |
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| Format: |
E-Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
2000 |
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| Schlagwörter: |
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| Anmerkung: |
© European Molecular Biology Organization 2000 |
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| Übergeordnetes Werk: |
Enthalten in: The EMBO Journal - Nature Publishing Group UK, 2023, 19(2000), 7 vom: 03. Apr., Seite 1450-1457 |
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| Übergeordnetes Werk: |
volume:19 ; year:2000 ; number:7 ; day:03 ; month:04 ; pages:1450-1457 |
| Links: |
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| DOI / URN: |
10.1093/emboj/19.7.1450 |
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| Katalog-ID: |
SPR057832110 |
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| 100 | 1 | |a Xia, Yan |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Regulatory cross‐talk between adhesin operons in Escherichia coli: inhibition of type 1 fimbriae expression by the PapB protein |
| 264 | 1 | |c 2000 | |
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| 500 | |a © European Molecular Biology Organization 2000 | ||
| 520 | |a Abstract Pathogenic Escherichia coli often carry determinants for several different adhesins. We show a direct communication between two adhesin gene clusters in uropathogenic E.coli: type 1 fimbriae (fim) and pyelonephritis‐associated pili (pap). A regulator of pap, PapB, is a key factor in this cross‐talk. FimB recombinase turns on type 1 fimbrial expression, and PapB inhibited phase transition by FimB in both off‐to‐on and on‐to‐off directions. On‐to‐off switching requiring FimE was increased by PapB. By analysis of FimB– and FimE–LacZ translational fusions it was concluded that the increase in on‐to‐off transition rates was via an increase in FimE expression. Inhibition of FimB‐promoted switching was via a different mechanism: PapB inhibited FimB‐promoted in vitro recombination, indicating that FimB activity was blocked at the fim switch. In vitro analyses showed that PapB bound to several DNA regions of the type 1 fimbrial operon, including the fim switch region. These data show that Pap expression turns off type 1 fimbriae expression in the same cell. Such cross‐talk between adhesin gene clusters may bring about appropriate expression at the single cell level. | ||
| 650 | 4 | |a adhesion |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a cross‐talk |7 (dpeaa)DE-He213 | |
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| 650 | 4 | |a phase switch |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a regulation |7 (dpeaa)DE-He213 | |
| 700 | 1 | |a Gally, David |e verfasserin |4 aut | |
| 700 | 1 | |a Forsman‐Semb, Kristina |e verfasserin |4 aut | |
| 700 | 1 | |a Uhlin, Bernt Eric |e verfasserin |4 aut | |
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| 952 | |d 19 |j 2000 |e 7 |b 03 |c 04 |h 1450-1457 | ||
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10.1093/emboj/19.7.1450 doi (DE-627)SPR057832110 (SPR)19.7.1450-e DE-627 ger DE-627 rakwb eng Xia, Yan verfasserin aut Regulatory cross‐talk between adhesin operons in Escherichia coli: inhibition of type 1 fimbriae expression by the PapB protein 2000 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © European Molecular Biology Organization 2000 Abstract Pathogenic Escherichia coli often carry determinants for several different adhesins. We show a direct communication between two adhesin gene clusters in uropathogenic E.coli: type 1 fimbriae (fim) and pyelonephritis‐associated pili (pap). A regulator of pap, PapB, is a key factor in this cross‐talk. FimB recombinase turns on type 1 fimbrial expression, and PapB inhibited phase transition by FimB in both off‐to‐on and on‐to‐off directions. On‐to‐off switching requiring FimE was increased by PapB. By analysis of FimB– and FimE–LacZ translational fusions it was concluded that the increase in on‐to‐off transition rates was via an increase in FimE expression. Inhibition of FimB‐promoted switching was via a different mechanism: PapB inhibited FimB‐promoted in vitro recombination, indicating that FimB activity was blocked at the fim switch. In vitro analyses showed that PapB bound to several DNA regions of the type 1 fimbrial operon, including the fim switch region. These data show that Pap expression turns off type 1 fimbriae expression in the same cell. Such cross‐talk between adhesin gene clusters may bring about appropriate expression at the single cell level. adhesion (dpeaa)DE-He213 cross‐talk (dpeaa)DE-He213 fimbriae (dpeaa)DE-He213 phase switch (dpeaa)DE-He213 regulation (dpeaa)DE-He213 Gally, David verfasserin aut Forsman‐Semb, Kristina verfasserin aut Uhlin, Bernt Eric verfasserin aut Enthalten in The EMBO Journal Nature Publishing Group UK, 2023 19(2000), 7 vom: 03. Apr., Seite 1450-1457 (DE-627)266022529 (DE-600)1467419-1 1460-2075 nnns volume:19 year:2000 number:7 day:03 month:04 pages:1450-1457 https://dx.doi.org/10.1093/emboj/19.7.1450 X:SPRINGER Resolving-System lizenzpflichtig Volltext SYSFLAG_0 GBV_SPRINGER GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_72 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_161 GBV_ILN_168 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_252 GBV_ILN_266 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2018 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4029 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4155 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4393 GBV_ILN_4700 AR 19 2000 7 03 04 1450-1457 |
| spelling |
10.1093/emboj/19.7.1450 doi (DE-627)SPR057832110 (SPR)19.7.1450-e DE-627 ger DE-627 rakwb eng Xia, Yan verfasserin aut Regulatory cross‐talk between adhesin operons in Escherichia coli: inhibition of type 1 fimbriae expression by the PapB protein 2000 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © European Molecular Biology Organization 2000 Abstract Pathogenic Escherichia coli often carry determinants for several different adhesins. We show a direct communication between two adhesin gene clusters in uropathogenic E.coli: type 1 fimbriae (fim) and pyelonephritis‐associated pili (pap). A regulator of pap, PapB, is a key factor in this cross‐talk. FimB recombinase turns on type 1 fimbrial expression, and PapB inhibited phase transition by FimB in both off‐to‐on and on‐to‐off directions. On‐to‐off switching requiring FimE was increased by PapB. By analysis of FimB– and FimE–LacZ translational fusions it was concluded that the increase in on‐to‐off transition rates was via an increase in FimE expression. Inhibition of FimB‐promoted switching was via a different mechanism: PapB inhibited FimB‐promoted in vitro recombination, indicating that FimB activity was blocked at the fim switch. In vitro analyses showed that PapB bound to several DNA regions of the type 1 fimbrial operon, including the fim switch region. These data show that Pap expression turns off type 1 fimbriae expression in the same cell. Such cross‐talk between adhesin gene clusters may bring about appropriate expression at the single cell level. adhesion (dpeaa)DE-He213 cross‐talk (dpeaa)DE-He213 fimbriae (dpeaa)DE-He213 phase switch (dpeaa)DE-He213 regulation (dpeaa)DE-He213 Gally, David verfasserin aut Forsman‐Semb, Kristina verfasserin aut Uhlin, Bernt Eric verfasserin aut Enthalten in The EMBO Journal Nature Publishing Group UK, 2023 19(2000), 7 vom: 03. Apr., Seite 1450-1457 (DE-627)266022529 (DE-600)1467419-1 1460-2075 nnns volume:19 year:2000 number:7 day:03 month:04 pages:1450-1457 https://dx.doi.org/10.1093/emboj/19.7.1450 X:SPRINGER Resolving-System lizenzpflichtig Volltext SYSFLAG_0 GBV_SPRINGER GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_72 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_161 GBV_ILN_168 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_252 GBV_ILN_266 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2018 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4029 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4155 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4393 GBV_ILN_4700 AR 19 2000 7 03 04 1450-1457 |
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10.1093/emboj/19.7.1450 doi (DE-627)SPR057832110 (SPR)19.7.1450-e DE-627 ger DE-627 rakwb eng Xia, Yan verfasserin aut Regulatory cross‐talk between adhesin operons in Escherichia coli: inhibition of type 1 fimbriae expression by the PapB protein 2000 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © European Molecular Biology Organization 2000 Abstract Pathogenic Escherichia coli often carry determinants for several different adhesins. We show a direct communication between two adhesin gene clusters in uropathogenic E.coli: type 1 fimbriae (fim) and pyelonephritis‐associated pili (pap). A regulator of pap, PapB, is a key factor in this cross‐talk. FimB recombinase turns on type 1 fimbrial expression, and PapB inhibited phase transition by FimB in both off‐to‐on and on‐to‐off directions. On‐to‐off switching requiring FimE was increased by PapB. By analysis of FimB– and FimE–LacZ translational fusions it was concluded that the increase in on‐to‐off transition rates was via an increase in FimE expression. Inhibition of FimB‐promoted switching was via a different mechanism: PapB inhibited FimB‐promoted in vitro recombination, indicating that FimB activity was blocked at the fim switch. In vitro analyses showed that PapB bound to several DNA regions of the type 1 fimbrial operon, including the fim switch region. These data show that Pap expression turns off type 1 fimbriae expression in the same cell. Such cross‐talk between adhesin gene clusters may bring about appropriate expression at the single cell level. adhesion (dpeaa)DE-He213 cross‐talk (dpeaa)DE-He213 fimbriae (dpeaa)DE-He213 phase switch (dpeaa)DE-He213 regulation (dpeaa)DE-He213 Gally, David verfasserin aut Forsman‐Semb, Kristina verfasserin aut Uhlin, Bernt Eric verfasserin aut Enthalten in The EMBO Journal Nature Publishing Group UK, 2023 19(2000), 7 vom: 03. Apr., Seite 1450-1457 (DE-627)266022529 (DE-600)1467419-1 1460-2075 nnns volume:19 year:2000 number:7 day:03 month:04 pages:1450-1457 https://dx.doi.org/10.1093/emboj/19.7.1450 X:SPRINGER Resolving-System lizenzpflichtig Volltext SYSFLAG_0 GBV_SPRINGER GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_72 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_161 GBV_ILN_168 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_252 GBV_ILN_266 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2018 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4029 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4155 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4393 GBV_ILN_4700 AR 19 2000 7 03 04 1450-1457 |
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10.1093/emboj/19.7.1450 doi (DE-627)SPR057832110 (SPR)19.7.1450-e DE-627 ger DE-627 rakwb eng Xia, Yan verfasserin aut Regulatory cross‐talk between adhesin operons in Escherichia coli: inhibition of type 1 fimbriae expression by the PapB protein 2000 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © European Molecular Biology Organization 2000 Abstract Pathogenic Escherichia coli often carry determinants for several different adhesins. We show a direct communication between two adhesin gene clusters in uropathogenic E.coli: type 1 fimbriae (fim) and pyelonephritis‐associated pili (pap). A regulator of pap, PapB, is a key factor in this cross‐talk. FimB recombinase turns on type 1 fimbrial expression, and PapB inhibited phase transition by FimB in both off‐to‐on and on‐to‐off directions. On‐to‐off switching requiring FimE was increased by PapB. By analysis of FimB– and FimE–LacZ translational fusions it was concluded that the increase in on‐to‐off transition rates was via an increase in FimE expression. Inhibition of FimB‐promoted switching was via a different mechanism: PapB inhibited FimB‐promoted in vitro recombination, indicating that FimB activity was blocked at the fim switch. In vitro analyses showed that PapB bound to several DNA regions of the type 1 fimbrial operon, including the fim switch region. These data show that Pap expression turns off type 1 fimbriae expression in the same cell. Such cross‐talk between adhesin gene clusters may bring about appropriate expression at the single cell level. adhesion (dpeaa)DE-He213 cross‐talk (dpeaa)DE-He213 fimbriae (dpeaa)DE-He213 phase switch (dpeaa)DE-He213 regulation (dpeaa)DE-He213 Gally, David verfasserin aut Forsman‐Semb, Kristina verfasserin aut Uhlin, Bernt Eric verfasserin aut Enthalten in The EMBO Journal Nature Publishing Group UK, 2023 19(2000), 7 vom: 03. Apr., Seite 1450-1457 (DE-627)266022529 (DE-600)1467419-1 1460-2075 nnns volume:19 year:2000 number:7 day:03 month:04 pages:1450-1457 https://dx.doi.org/10.1093/emboj/19.7.1450 X:SPRINGER Resolving-System lizenzpflichtig Volltext SYSFLAG_0 GBV_SPRINGER GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_72 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_161 GBV_ILN_168 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_252 GBV_ILN_266 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2018 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4029 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4155 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4393 GBV_ILN_4700 AR 19 2000 7 03 04 1450-1457 |
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10.1093/emboj/19.7.1450 doi (DE-627)SPR057832110 (SPR)19.7.1450-e DE-627 ger DE-627 rakwb eng Xia, Yan verfasserin aut Regulatory cross‐talk between adhesin operons in Escherichia coli: inhibition of type 1 fimbriae expression by the PapB protein 2000 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © European Molecular Biology Organization 2000 Abstract Pathogenic Escherichia coli often carry determinants for several different adhesins. We show a direct communication between two adhesin gene clusters in uropathogenic E.coli: type 1 fimbriae (fim) and pyelonephritis‐associated pili (pap). A regulator of pap, PapB, is a key factor in this cross‐talk. FimB recombinase turns on type 1 fimbrial expression, and PapB inhibited phase transition by FimB in both off‐to‐on and on‐to‐off directions. On‐to‐off switching requiring FimE was increased by PapB. By analysis of FimB– and FimE–LacZ translational fusions it was concluded that the increase in on‐to‐off transition rates was via an increase in FimE expression. Inhibition of FimB‐promoted switching was via a different mechanism: PapB inhibited FimB‐promoted in vitro recombination, indicating that FimB activity was blocked at the fim switch. In vitro analyses showed that PapB bound to several DNA regions of the type 1 fimbrial operon, including the fim switch region. These data show that Pap expression turns off type 1 fimbriae expression in the same cell. Such cross‐talk between adhesin gene clusters may bring about appropriate expression at the single cell level. adhesion (dpeaa)DE-He213 cross‐talk (dpeaa)DE-He213 fimbriae (dpeaa)DE-He213 phase switch (dpeaa)DE-He213 regulation (dpeaa)DE-He213 Gally, David verfasserin aut Forsman‐Semb, Kristina verfasserin aut Uhlin, Bernt Eric verfasserin aut Enthalten in The EMBO Journal Nature Publishing Group UK, 2023 19(2000), 7 vom: 03. Apr., Seite 1450-1457 (DE-627)266022529 (DE-600)1467419-1 1460-2075 nnns volume:19 year:2000 number:7 day:03 month:04 pages:1450-1457 https://dx.doi.org/10.1093/emboj/19.7.1450 X:SPRINGER Resolving-System lizenzpflichtig Volltext SYSFLAG_0 GBV_SPRINGER GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_72 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_161 GBV_ILN_168 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_252 GBV_ILN_266 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2018 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4029 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4155 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4393 GBV_ILN_4700 AR 19 2000 7 03 04 1450-1457 |
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Xia, Yan @@aut@@ Gally, David @@aut@@ Forsman‐Semb, Kristina @@aut@@ Uhlin, Bernt Eric @@aut@@ |
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We show a direct communication between two adhesin gene clusters in uropathogenic E.coli: type 1 fimbriae (fim) and pyelonephritis‐associated pili (pap). A regulator of pap, PapB, is a key factor in this cross‐talk. FimB recombinase turns on type 1 fimbrial expression, and PapB inhibited phase transition by FimB in both off‐to‐on and on‐to‐off directions. On‐to‐off switching requiring FimE was increased by PapB. By analysis of FimB– and FimE–LacZ translational fusions it was concluded that the increase in on‐to‐off transition rates was via an increase in FimE expression. Inhibition of FimB‐promoted switching was via a different mechanism: PapB inhibited FimB‐promoted in vitro recombination, indicating that FimB activity was blocked at the fim switch. In vitro analyses showed that PapB bound to several DNA regions of the type 1 fimbrial operon, including the fim switch region. These data show that Pap expression turns off type 1 fimbriae expression in the same cell. 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Xia, Yan |
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Xia, Yan misc adhesion misc cross‐talk misc fimbriae misc phase switch misc regulation Regulatory cross‐talk between adhesin operons in Escherichia coli: inhibition of type 1 fimbriae expression by the PapB protein |
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Regulatory cross‐talk between adhesin operons in Escherichia coli: inhibition of type 1 fimbriae expression by the PapB protein adhesion (dpeaa)DE-He213 cross‐talk (dpeaa)DE-He213 fimbriae (dpeaa)DE-He213 phase switch (dpeaa)DE-He213 regulation (dpeaa)DE-He213 |
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Regulatory cross‐talk between adhesin operons in Escherichia coli: inhibition of type 1 fimbriae expression by the PapB protein |
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Regulatory cross‐talk between adhesin operons in Escherichia coli: inhibition of type 1 fimbriae expression by the PapB protein |
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Xia, Yan Gally, David Forsman‐Semb, Kristina Uhlin, Bernt Eric |
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regulatory cross‐talk between adhesin operons in escherichia coli: inhibition of type 1 fimbriae expression by the papb protein |
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Regulatory cross‐talk between adhesin operons in Escherichia coli: inhibition of type 1 fimbriae expression by the PapB protein |
| abstract |
Abstract Pathogenic Escherichia coli often carry determinants for several different adhesins. We show a direct communication between two adhesin gene clusters in uropathogenic E.coli: type 1 fimbriae (fim) and pyelonephritis‐associated pili (pap). A regulator of pap, PapB, is a key factor in this cross‐talk. FimB recombinase turns on type 1 fimbrial expression, and PapB inhibited phase transition by FimB in both off‐to‐on and on‐to‐off directions. On‐to‐off switching requiring FimE was increased by PapB. By analysis of FimB– and FimE–LacZ translational fusions it was concluded that the increase in on‐to‐off transition rates was via an increase in FimE expression. Inhibition of FimB‐promoted switching was via a different mechanism: PapB inhibited FimB‐promoted in vitro recombination, indicating that FimB activity was blocked at the fim switch. In vitro analyses showed that PapB bound to several DNA regions of the type 1 fimbrial operon, including the fim switch region. These data show that Pap expression turns off type 1 fimbriae expression in the same cell. Such cross‐talk between adhesin gene clusters may bring about appropriate expression at the single cell level. © European Molecular Biology Organization 2000 |
| abstractGer |
Abstract Pathogenic Escherichia coli often carry determinants for several different adhesins. We show a direct communication between two adhesin gene clusters in uropathogenic E.coli: type 1 fimbriae (fim) and pyelonephritis‐associated pili (pap). A regulator of pap, PapB, is a key factor in this cross‐talk. FimB recombinase turns on type 1 fimbrial expression, and PapB inhibited phase transition by FimB in both off‐to‐on and on‐to‐off directions. On‐to‐off switching requiring FimE was increased by PapB. By analysis of FimB– and FimE–LacZ translational fusions it was concluded that the increase in on‐to‐off transition rates was via an increase in FimE expression. Inhibition of FimB‐promoted switching was via a different mechanism: PapB inhibited FimB‐promoted in vitro recombination, indicating that FimB activity was blocked at the fim switch. In vitro analyses showed that PapB bound to several DNA regions of the type 1 fimbrial operon, including the fim switch region. These data show that Pap expression turns off type 1 fimbriae expression in the same cell. Such cross‐talk between adhesin gene clusters may bring about appropriate expression at the single cell level. © European Molecular Biology Organization 2000 |
| abstract_unstemmed |
Abstract Pathogenic Escherichia coli often carry determinants for several different adhesins. We show a direct communication between two adhesin gene clusters in uropathogenic E.coli: type 1 fimbriae (fim) and pyelonephritis‐associated pili (pap). A regulator of pap, PapB, is a key factor in this cross‐talk. FimB recombinase turns on type 1 fimbrial expression, and PapB inhibited phase transition by FimB in both off‐to‐on and on‐to‐off directions. On‐to‐off switching requiring FimE was increased by PapB. By analysis of FimB– and FimE–LacZ translational fusions it was concluded that the increase in on‐to‐off transition rates was via an increase in FimE expression. Inhibition of FimB‐promoted switching was via a different mechanism: PapB inhibited FimB‐promoted in vitro recombination, indicating that FimB activity was blocked at the fim switch. In vitro analyses showed that PapB bound to several DNA regions of the type 1 fimbrial operon, including the fim switch region. These data show that Pap expression turns off type 1 fimbriae expression in the same cell. Such cross‐talk between adhesin gene clusters may bring about appropriate expression at the single cell level. © European Molecular Biology Organization 2000 |
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| title_short |
Regulatory cross‐talk between adhesin operons in Escherichia coli: inhibition of type 1 fimbriae expression by the PapB protein |
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https://dx.doi.org/10.1093/emboj/19.7.1450 |
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Gally, David Forsman‐Semb, Kristina Uhlin, Bernt Eric |
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10.1093/emboj/19.7.1450 |
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2024-10-17T04:51:15.591Z |
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| score |
7.3995953 |