MutS mediates heteroduplex loop formation by a translocation mechanism
Abstract Interaction of Escherichia coli MutS and MutL with heteroduplex DNA has been visualized by electron microscopy. In a reaction dependent on ATP hydrolysis, complexes between a MutS dimer and a DNA heteroduplex are converted to protein‐stabilized, α‐shaped loop structures with the mismatch in...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
Allen, Dwayne J. [verfasserIn] Makhov, Alexander [verfasserIn] Grilley, Michelle [verfasserIn] Taylor, John [verfasserIn] Thresher, Randy [verfasserIn] Modrich, Paul [verfasserIn] Griffith, Jack D. [verfasserIn] |
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| Format: |
E-Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
1997 |
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| Schlagwörter: |
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| Anmerkung: |
© European Molecular Biology Organization 1997 |
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| Übergeordnetes Werk: |
Enthalten in: The EMBO Journal - Nature Publishing Group UK, 2023, 16(1997), 14 vom: 15. Juli, Seite 4467-4476 |
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| Übergeordnetes Werk: |
volume:16 ; year:1997 ; number:14 ; day:15 ; month:07 ; pages:4467-4476 |
| Links: |
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| DOI / URN: |
10.1093/emboj/16.14.4467 |
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| Katalog-ID: |
SPR057850844 |
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| 100 | 1 | |a Allen, Dwayne J. |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a MutS mediates heteroduplex loop formation by a translocation mechanism |
| 264 | 1 | |c 1997 | |
| 336 | |a Text |b txt |2 rdacontent | ||
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| 500 | |a © European Molecular Biology Organization 1997 | ||
| 520 | |a Abstract Interaction of Escherichia coli MutS and MutL with heteroduplex DNA has been visualized by electron microscopy. In a reaction dependent on ATP hydrolysis, complexes between a MutS dimer and a DNA heteroduplex are converted to protein‐stabilized, α‐shaped loop structures with the mismatch in most cases located within the DNA loop. Loop formation depends on ATP hydrolysis and loop size increases linearly with time at a rate of 370 base pairs/min in phosphate buffer and about 10 000 base pairs/min in the HEPES buffer used for repair assay. These observations suggest a translocation mechanism in which a MutS dimer bound to a mismatch subsequently leaves this site by ATP‐dependent tracking or unidimensional movement that is in most cases bidirectional from the mispair. In view of the bidirectional capability of the methyl‐directed pathway, this reaction may play a role in determination of heteroduplex orientation. The rate of MutS‐mediated DNA loop growth is enhanced by MutL, and when both proteins are present, both are found at the base of α‐loop structures, and both can remain associated with excision intermediates produced in later stages of the reaction. | ||
| 650 | 4 | |a DNA repair |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a mismatch repair |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a mutagenesis |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a mutator |7 (dpeaa)DE-He213 | |
| 700 | 1 | |a Makhov, Alexander |e verfasserin |4 aut | |
| 700 | 1 | |a Grilley, Michelle |e verfasserin |4 aut | |
| 700 | 1 | |a Taylor, John |e verfasserin |4 aut | |
| 700 | 1 | |a Thresher, Randy |e verfasserin |4 aut | |
| 700 | 1 | |a Modrich, Paul |e verfasserin |4 aut | |
| 700 | 1 | |a Griffith, Jack D. |e verfasserin |4 aut | |
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| 912 | |a GBV_ILN_2049 | ||
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| 912 | |a GBV_ILN_2055 | ||
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| 912 | |a GBV_ILN_2059 | ||
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| 912 | |a GBV_ILN_2064 | ||
| 912 | |a GBV_ILN_2068 | ||
| 912 | |a GBV_ILN_2088 | ||
| 912 | |a GBV_ILN_2093 | ||
| 912 | |a GBV_ILN_2106 | ||
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| 951 | |a AR | ||
| 952 | |d 16 |j 1997 |e 14 |b 15 |c 07 |h 4467-4476 | ||
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1997 |
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1997 |
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10.1093/emboj/16.14.4467 doi (DE-627)SPR057850844 (SPR)16.14.4467-e DE-627 ger DE-627 rakwb eng Allen, Dwayne J. verfasserin aut MutS mediates heteroduplex loop formation by a translocation mechanism 1997 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © European Molecular Biology Organization 1997 Abstract Interaction of Escherichia coli MutS and MutL with heteroduplex DNA has been visualized by electron microscopy. In a reaction dependent on ATP hydrolysis, complexes between a MutS dimer and a DNA heteroduplex are converted to protein‐stabilized, α‐shaped loop structures with the mismatch in most cases located within the DNA loop. Loop formation depends on ATP hydrolysis and loop size increases linearly with time at a rate of 370 base pairs/min in phosphate buffer and about 10 000 base pairs/min in the HEPES buffer used for repair assay. These observations suggest a translocation mechanism in which a MutS dimer bound to a mismatch subsequently leaves this site by ATP‐dependent tracking or unidimensional movement that is in most cases bidirectional from the mispair. In view of the bidirectional capability of the methyl‐directed pathway, this reaction may play a role in determination of heteroduplex orientation. The rate of MutS‐mediated DNA loop growth is enhanced by MutL, and when both proteins are present, both are found at the base of α‐loop structures, and both can remain associated with excision intermediates produced in later stages of the reaction. DNA repair (dpeaa)DE-He213 mismatch repair (dpeaa)DE-He213 mutagenesis (dpeaa)DE-He213 mutator (dpeaa)DE-He213 Makhov, Alexander verfasserin aut Grilley, Michelle verfasserin aut Taylor, John verfasserin aut Thresher, Randy verfasserin aut Modrich, Paul verfasserin aut Griffith, Jack D. verfasserin aut Enthalten in The EMBO Journal Nature Publishing Group UK, 2023 16(1997), 14 vom: 15. Juli, Seite 4467-4476 (DE-627)266022529 (DE-600)1467419-1 1460-2075 nnns volume:16 year:1997 number:14 day:15 month:07 pages:4467-4476 https://dx.doi.org/10.1093/emboj/16.14.4467 X:SPRINGER Resolving-System lizenzpflichtig Volltext SYSFLAG_0 GBV_SPRINGER GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_72 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_161 GBV_ILN_168 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_252 GBV_ILN_266 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2018 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4029 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4155 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4393 GBV_ILN_4700 AR 16 1997 14 15 07 4467-4476 |
| spelling |
10.1093/emboj/16.14.4467 doi (DE-627)SPR057850844 (SPR)16.14.4467-e DE-627 ger DE-627 rakwb eng Allen, Dwayne J. verfasserin aut MutS mediates heteroduplex loop formation by a translocation mechanism 1997 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © European Molecular Biology Organization 1997 Abstract Interaction of Escherichia coli MutS and MutL with heteroduplex DNA has been visualized by electron microscopy. In a reaction dependent on ATP hydrolysis, complexes between a MutS dimer and a DNA heteroduplex are converted to protein‐stabilized, α‐shaped loop structures with the mismatch in most cases located within the DNA loop. Loop formation depends on ATP hydrolysis and loop size increases linearly with time at a rate of 370 base pairs/min in phosphate buffer and about 10 000 base pairs/min in the HEPES buffer used for repair assay. These observations suggest a translocation mechanism in which a MutS dimer bound to a mismatch subsequently leaves this site by ATP‐dependent tracking or unidimensional movement that is in most cases bidirectional from the mispair. In view of the bidirectional capability of the methyl‐directed pathway, this reaction may play a role in determination of heteroduplex orientation. The rate of MutS‐mediated DNA loop growth is enhanced by MutL, and when both proteins are present, both are found at the base of α‐loop structures, and both can remain associated with excision intermediates produced in later stages of the reaction. DNA repair (dpeaa)DE-He213 mismatch repair (dpeaa)DE-He213 mutagenesis (dpeaa)DE-He213 mutator (dpeaa)DE-He213 Makhov, Alexander verfasserin aut Grilley, Michelle verfasserin aut Taylor, John verfasserin aut Thresher, Randy verfasserin aut Modrich, Paul verfasserin aut Griffith, Jack D. verfasserin aut Enthalten in The EMBO Journal Nature Publishing Group UK, 2023 16(1997), 14 vom: 15. Juli, Seite 4467-4476 (DE-627)266022529 (DE-600)1467419-1 1460-2075 nnns volume:16 year:1997 number:14 day:15 month:07 pages:4467-4476 https://dx.doi.org/10.1093/emboj/16.14.4467 X:SPRINGER Resolving-System lizenzpflichtig Volltext SYSFLAG_0 GBV_SPRINGER GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_72 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_161 GBV_ILN_168 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_252 GBV_ILN_266 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2018 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4029 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4155 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4393 GBV_ILN_4700 AR 16 1997 14 15 07 4467-4476 |
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10.1093/emboj/16.14.4467 doi (DE-627)SPR057850844 (SPR)16.14.4467-e DE-627 ger DE-627 rakwb eng Allen, Dwayne J. verfasserin aut MutS mediates heteroduplex loop formation by a translocation mechanism 1997 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © European Molecular Biology Organization 1997 Abstract Interaction of Escherichia coli MutS and MutL with heteroduplex DNA has been visualized by electron microscopy. In a reaction dependent on ATP hydrolysis, complexes between a MutS dimer and a DNA heteroduplex are converted to protein‐stabilized, α‐shaped loop structures with the mismatch in most cases located within the DNA loop. Loop formation depends on ATP hydrolysis and loop size increases linearly with time at a rate of 370 base pairs/min in phosphate buffer and about 10 000 base pairs/min in the HEPES buffer used for repair assay. These observations suggest a translocation mechanism in which a MutS dimer bound to a mismatch subsequently leaves this site by ATP‐dependent tracking or unidimensional movement that is in most cases bidirectional from the mispair. In view of the bidirectional capability of the methyl‐directed pathway, this reaction may play a role in determination of heteroduplex orientation. The rate of MutS‐mediated DNA loop growth is enhanced by MutL, and when both proteins are present, both are found at the base of α‐loop structures, and both can remain associated with excision intermediates produced in later stages of the reaction. DNA repair (dpeaa)DE-He213 mismatch repair (dpeaa)DE-He213 mutagenesis (dpeaa)DE-He213 mutator (dpeaa)DE-He213 Makhov, Alexander verfasserin aut Grilley, Michelle verfasserin aut Taylor, John verfasserin aut Thresher, Randy verfasserin aut Modrich, Paul verfasserin aut Griffith, Jack D. verfasserin aut Enthalten in The EMBO Journal Nature Publishing Group UK, 2023 16(1997), 14 vom: 15. Juli, Seite 4467-4476 (DE-627)266022529 (DE-600)1467419-1 1460-2075 nnns volume:16 year:1997 number:14 day:15 month:07 pages:4467-4476 https://dx.doi.org/10.1093/emboj/16.14.4467 X:SPRINGER Resolving-System lizenzpflichtig Volltext SYSFLAG_0 GBV_SPRINGER GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_72 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_161 GBV_ILN_168 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_252 GBV_ILN_266 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2018 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4029 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4155 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4393 GBV_ILN_4700 AR 16 1997 14 15 07 4467-4476 |
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10.1093/emboj/16.14.4467 doi (DE-627)SPR057850844 (SPR)16.14.4467-e DE-627 ger DE-627 rakwb eng Allen, Dwayne J. verfasserin aut MutS mediates heteroduplex loop formation by a translocation mechanism 1997 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © European Molecular Biology Organization 1997 Abstract Interaction of Escherichia coli MutS and MutL with heteroduplex DNA has been visualized by electron microscopy. In a reaction dependent on ATP hydrolysis, complexes between a MutS dimer and a DNA heteroduplex are converted to protein‐stabilized, α‐shaped loop structures with the mismatch in most cases located within the DNA loop. Loop formation depends on ATP hydrolysis and loop size increases linearly with time at a rate of 370 base pairs/min in phosphate buffer and about 10 000 base pairs/min in the HEPES buffer used for repair assay. These observations suggest a translocation mechanism in which a MutS dimer bound to a mismatch subsequently leaves this site by ATP‐dependent tracking or unidimensional movement that is in most cases bidirectional from the mispair. In view of the bidirectional capability of the methyl‐directed pathway, this reaction may play a role in determination of heteroduplex orientation. The rate of MutS‐mediated DNA loop growth is enhanced by MutL, and when both proteins are present, both are found at the base of α‐loop structures, and both can remain associated with excision intermediates produced in later stages of the reaction. DNA repair (dpeaa)DE-He213 mismatch repair (dpeaa)DE-He213 mutagenesis (dpeaa)DE-He213 mutator (dpeaa)DE-He213 Makhov, Alexander verfasserin aut Grilley, Michelle verfasserin aut Taylor, John verfasserin aut Thresher, Randy verfasserin aut Modrich, Paul verfasserin aut Griffith, Jack D. verfasserin aut Enthalten in The EMBO Journal Nature Publishing Group UK, 2023 16(1997), 14 vom: 15. Juli, Seite 4467-4476 (DE-627)266022529 (DE-600)1467419-1 1460-2075 nnns volume:16 year:1997 number:14 day:15 month:07 pages:4467-4476 https://dx.doi.org/10.1093/emboj/16.14.4467 X:SPRINGER Resolving-System lizenzpflichtig Volltext SYSFLAG_0 GBV_SPRINGER GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_72 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_161 GBV_ILN_168 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_252 GBV_ILN_266 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2018 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4029 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4155 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4393 GBV_ILN_4700 AR 16 1997 14 15 07 4467-4476 |
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10.1093/emboj/16.14.4467 doi (DE-627)SPR057850844 (SPR)16.14.4467-e DE-627 ger DE-627 rakwb eng Allen, Dwayne J. verfasserin aut MutS mediates heteroduplex loop formation by a translocation mechanism 1997 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © European Molecular Biology Organization 1997 Abstract Interaction of Escherichia coli MutS and MutL with heteroduplex DNA has been visualized by electron microscopy. In a reaction dependent on ATP hydrolysis, complexes between a MutS dimer and a DNA heteroduplex are converted to protein‐stabilized, α‐shaped loop structures with the mismatch in most cases located within the DNA loop. Loop formation depends on ATP hydrolysis and loop size increases linearly with time at a rate of 370 base pairs/min in phosphate buffer and about 10 000 base pairs/min in the HEPES buffer used for repair assay. These observations suggest a translocation mechanism in which a MutS dimer bound to a mismatch subsequently leaves this site by ATP‐dependent tracking or unidimensional movement that is in most cases bidirectional from the mispair. In view of the bidirectional capability of the methyl‐directed pathway, this reaction may play a role in determination of heteroduplex orientation. The rate of MutS‐mediated DNA loop growth is enhanced by MutL, and when both proteins are present, both are found at the base of α‐loop structures, and both can remain associated with excision intermediates produced in later stages of the reaction. DNA repair (dpeaa)DE-He213 mismatch repair (dpeaa)DE-He213 mutagenesis (dpeaa)DE-He213 mutator (dpeaa)DE-He213 Makhov, Alexander verfasserin aut Grilley, Michelle verfasserin aut Taylor, John verfasserin aut Thresher, Randy verfasserin aut Modrich, Paul verfasserin aut Griffith, Jack D. verfasserin aut Enthalten in The EMBO Journal Nature Publishing Group UK, 2023 16(1997), 14 vom: 15. Juli, Seite 4467-4476 (DE-627)266022529 (DE-600)1467419-1 1460-2075 nnns volume:16 year:1997 number:14 day:15 month:07 pages:4467-4476 https://dx.doi.org/10.1093/emboj/16.14.4467 X:SPRINGER Resolving-System lizenzpflichtig Volltext SYSFLAG_0 GBV_SPRINGER GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_72 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_161 GBV_ILN_168 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_252 GBV_ILN_266 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2018 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4029 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4155 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4393 GBV_ILN_4700 AR 16 1997 14 15 07 4467-4476 |
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Enthalten in The EMBO Journal 16(1997), 14 vom: 15. Juli, Seite 4467-4476 volume:16 year:1997 number:14 day:15 month:07 pages:4467-4476 |
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Allen, Dwayne J. @@aut@@ Makhov, Alexander @@aut@@ Grilley, Michelle @@aut@@ Taylor, John @@aut@@ Thresher, Randy @@aut@@ Modrich, Paul @@aut@@ Griffith, Jack D. @@aut@@ |
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Allen, Dwayne J. |
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Allen, Dwayne J. misc DNA repair misc mismatch repair misc mutagenesis misc mutator MutS mediates heteroduplex loop formation by a translocation mechanism |
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MutS mediates heteroduplex loop formation by a translocation mechanism DNA repair (dpeaa)DE-He213 mismatch repair (dpeaa)DE-He213 mutagenesis (dpeaa)DE-He213 mutator (dpeaa)DE-He213 |
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muts mediates heteroduplex loop formation by a translocation mechanism |
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MutS mediates heteroduplex loop formation by a translocation mechanism |
| abstract |
Abstract Interaction of Escherichia coli MutS and MutL with heteroduplex DNA has been visualized by electron microscopy. In a reaction dependent on ATP hydrolysis, complexes between a MutS dimer and a DNA heteroduplex are converted to protein‐stabilized, α‐shaped loop structures with the mismatch in most cases located within the DNA loop. Loop formation depends on ATP hydrolysis and loop size increases linearly with time at a rate of 370 base pairs/min in phosphate buffer and about 10 000 base pairs/min in the HEPES buffer used for repair assay. These observations suggest a translocation mechanism in which a MutS dimer bound to a mismatch subsequently leaves this site by ATP‐dependent tracking or unidimensional movement that is in most cases bidirectional from the mispair. In view of the bidirectional capability of the methyl‐directed pathway, this reaction may play a role in determination of heteroduplex orientation. The rate of MutS‐mediated DNA loop growth is enhanced by MutL, and when both proteins are present, both are found at the base of α‐loop structures, and both can remain associated with excision intermediates produced in later stages of the reaction. © European Molecular Biology Organization 1997 |
| abstractGer |
Abstract Interaction of Escherichia coli MutS and MutL with heteroduplex DNA has been visualized by electron microscopy. In a reaction dependent on ATP hydrolysis, complexes between a MutS dimer and a DNA heteroduplex are converted to protein‐stabilized, α‐shaped loop structures with the mismatch in most cases located within the DNA loop. Loop formation depends on ATP hydrolysis and loop size increases linearly with time at a rate of 370 base pairs/min in phosphate buffer and about 10 000 base pairs/min in the HEPES buffer used for repair assay. These observations suggest a translocation mechanism in which a MutS dimer bound to a mismatch subsequently leaves this site by ATP‐dependent tracking or unidimensional movement that is in most cases bidirectional from the mispair. In view of the bidirectional capability of the methyl‐directed pathway, this reaction may play a role in determination of heteroduplex orientation. The rate of MutS‐mediated DNA loop growth is enhanced by MutL, and when both proteins are present, both are found at the base of α‐loop structures, and both can remain associated with excision intermediates produced in later stages of the reaction. © European Molecular Biology Organization 1997 |
| abstract_unstemmed |
Abstract Interaction of Escherichia coli MutS and MutL with heteroduplex DNA has been visualized by electron microscopy. In a reaction dependent on ATP hydrolysis, complexes between a MutS dimer and a DNA heteroduplex are converted to protein‐stabilized, α‐shaped loop structures with the mismatch in most cases located within the DNA loop. Loop formation depends on ATP hydrolysis and loop size increases linearly with time at a rate of 370 base pairs/min in phosphate buffer and about 10 000 base pairs/min in the HEPES buffer used for repair assay. These observations suggest a translocation mechanism in which a MutS dimer bound to a mismatch subsequently leaves this site by ATP‐dependent tracking or unidimensional movement that is in most cases bidirectional from the mispair. In view of the bidirectional capability of the methyl‐directed pathway, this reaction may play a role in determination of heteroduplex orientation. The rate of MutS‐mediated DNA loop growth is enhanced by MutL, and when both proteins are present, both are found at the base of α‐loop structures, and both can remain associated with excision intermediates produced in later stages of the reaction. © European Molecular Biology Organization 1997 |
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MutS mediates heteroduplex loop formation by a translocation mechanism |
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Makhov, Alexander Grilley, Michelle Taylor, John Thresher, Randy Modrich, Paul Griffith, Jack D. |
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2024-10-18T04:52:35.814Z |
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| score |
7.399392 |