WGEF activates Rho in the Wnt–PCP pathway and controls convergent extension in Xenopus gastrulation
Abstract The Wnt–PCP (planar cell polarity, PCP) pathway regulates cell polarity and convergent extension movements during axis formation in vertebrates by activation of Rho and Rac, leading to the re‐organization of the actin cytoskeleton. Rho and Rac activation require guanine nucleotide‐exchange...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
Tanegashima, Kosuke [verfasserIn] Zhao, Hui [verfasserIn] Dawid, Igor B [verfasserIn] |
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| Format: |
E-Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
2008 |
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| Schlagwörter: |
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| Anmerkung: |
© European Molecular Biology Organization 2008 |
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| Übergeordnetes Werk: |
Enthalten in: The EMBO Journal - Nature Publishing Group UK, 2023, 27(2008), 4 vom: 07. Feb., Seite 606-617 |
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| Übergeordnetes Werk: |
volume:27 ; year:2008 ; number:4 ; day:07 ; month:02 ; pages:606-617 |
| Links: |
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| DOI / URN: |
10.1038/emboj.2008.9 |
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| Katalog-ID: |
SPR057860270 |
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| 100 | 1 | |a Tanegashima, Kosuke |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a WGEF activates Rho in the Wnt–PCP pathway and controls convergent extension in Xenopus gastrulation |
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| 520 | |a Abstract The Wnt–PCP (planar cell polarity, PCP) pathway regulates cell polarity and convergent extension movements during axis formation in vertebrates by activation of Rho and Rac, leading to the re‐organization of the actin cytoskeleton. Rho and Rac activation require guanine nucleotide‐exchange factors (GEFs), but the identity of the GEF involved in Wnt–PCP‐mediated convergent extension is unknown. Here we report the identification of the weak‐similarity GEF (WGEF) gene by a microarray‐based screen for notochord enriched genes, and show that WGEF is involved in Wnt‐regulated convergent extension. Overexpression of WGEF activated RhoA and rescued the suppression of convergent extension by dominant‐negative Wnt‐11, whereas depletion of WGEF led to suppression of convergent extension that could be rescued by RhoA or Rho‐associated kinase activation. WGEF protein preferentially localized at the plasma membrane, and Frizzled‐7 induced colocalization of Dishevelled and WGEF. WGEF protein can bind to Dishevelled and Daam‐1, and deletion of the Dishevelled‐binding domain generates a hyperactive from of WGEF. These results indicate that WGEF is a component of the Wnt–PCP pathway that connects Dishevelled to Rho activation. | ||
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| 650 | 4 | |a gastrulation |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a GEF |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a Wnt–PCP |7 (dpeaa)DE-He213 | |
| 700 | 1 | |a Zhao, Hui |e verfasserin |4 aut | |
| 700 | 1 | |a Dawid, Igor B |e verfasserin |4 aut | |
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10.1038/emboj.2008.9 doi (DE-627)SPR057860270 (SPR)emboj.2008.9-e DE-627 ger DE-627 rakwb eng Tanegashima, Kosuke verfasserin aut WGEF activates Rho in the Wnt–PCP pathway and controls convergent extension in Xenopus gastrulation 2008 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © European Molecular Biology Organization 2008 Abstract The Wnt–PCP (planar cell polarity, PCP) pathway regulates cell polarity and convergent extension movements during axis formation in vertebrates by activation of Rho and Rac, leading to the re‐organization of the actin cytoskeleton. Rho and Rac activation require guanine nucleotide‐exchange factors (GEFs), but the identity of the GEF involved in Wnt–PCP‐mediated convergent extension is unknown. Here we report the identification of the weak‐similarity GEF (WGEF) gene by a microarray‐based screen for notochord enriched genes, and show that WGEF is involved in Wnt‐regulated convergent extension. Overexpression of WGEF activated RhoA and rescued the suppression of convergent extension by dominant‐negative Wnt‐11, whereas depletion of WGEF led to suppression of convergent extension that could be rescued by RhoA or Rho‐associated kinase activation. WGEF protein preferentially localized at the plasma membrane, and Frizzled‐7 induced colocalization of Dishevelled and WGEF. WGEF protein can bind to Dishevelled and Daam‐1, and deletion of the Dishevelled‐binding domain generates a hyperactive from of WGEF. These results indicate that WGEF is a component of the Wnt–PCP pathway that connects Dishevelled to Rho activation. convergent extension (dpeaa)DE-He213 gastrulation (dpeaa)DE-He213 GEF (dpeaa)DE-He213 Wnt–PCP (dpeaa)DE-He213 Zhao, Hui verfasserin aut Dawid, Igor B verfasserin aut Enthalten in The EMBO Journal Nature Publishing Group UK, 2023 27(2008), 4 vom: 07. Feb., Seite 606-617 (DE-627)266022529 (DE-600)1467419-1 1460-2075 nnns volume:27 year:2008 number:4 day:07 month:02 pages:606-617 https://dx.doi.org/10.1038/emboj.2008.9 X:SPRINGER Resolving-System lizenzpflichtig Volltext SYSFLAG_0 GBV_SPRINGER GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_72 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_161 GBV_ILN_168 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_252 GBV_ILN_266 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2018 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4029 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4155 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4393 GBV_ILN_4700 AR 27 2008 4 07 02 606-617 |
| spelling |
10.1038/emboj.2008.9 doi (DE-627)SPR057860270 (SPR)emboj.2008.9-e DE-627 ger DE-627 rakwb eng Tanegashima, Kosuke verfasserin aut WGEF activates Rho in the Wnt–PCP pathway and controls convergent extension in Xenopus gastrulation 2008 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © European Molecular Biology Organization 2008 Abstract The Wnt–PCP (planar cell polarity, PCP) pathway regulates cell polarity and convergent extension movements during axis formation in vertebrates by activation of Rho and Rac, leading to the re‐organization of the actin cytoskeleton. Rho and Rac activation require guanine nucleotide‐exchange factors (GEFs), but the identity of the GEF involved in Wnt–PCP‐mediated convergent extension is unknown. Here we report the identification of the weak‐similarity GEF (WGEF) gene by a microarray‐based screen for notochord enriched genes, and show that WGEF is involved in Wnt‐regulated convergent extension. Overexpression of WGEF activated RhoA and rescued the suppression of convergent extension by dominant‐negative Wnt‐11, whereas depletion of WGEF led to suppression of convergent extension that could be rescued by RhoA or Rho‐associated kinase activation. WGEF protein preferentially localized at the plasma membrane, and Frizzled‐7 induced colocalization of Dishevelled and WGEF. WGEF protein can bind to Dishevelled and Daam‐1, and deletion of the Dishevelled‐binding domain generates a hyperactive from of WGEF. These results indicate that WGEF is a component of the Wnt–PCP pathway that connects Dishevelled to Rho activation. convergent extension (dpeaa)DE-He213 gastrulation (dpeaa)DE-He213 GEF (dpeaa)DE-He213 Wnt–PCP (dpeaa)DE-He213 Zhao, Hui verfasserin aut Dawid, Igor B verfasserin aut Enthalten in The EMBO Journal Nature Publishing Group UK, 2023 27(2008), 4 vom: 07. Feb., Seite 606-617 (DE-627)266022529 (DE-600)1467419-1 1460-2075 nnns volume:27 year:2008 number:4 day:07 month:02 pages:606-617 https://dx.doi.org/10.1038/emboj.2008.9 X:SPRINGER Resolving-System lizenzpflichtig Volltext SYSFLAG_0 GBV_SPRINGER GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_72 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_161 GBV_ILN_168 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_252 GBV_ILN_266 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2018 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4029 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4155 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4393 GBV_ILN_4700 AR 27 2008 4 07 02 606-617 |
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10.1038/emboj.2008.9 doi (DE-627)SPR057860270 (SPR)emboj.2008.9-e DE-627 ger DE-627 rakwb eng Tanegashima, Kosuke verfasserin aut WGEF activates Rho in the Wnt–PCP pathway and controls convergent extension in Xenopus gastrulation 2008 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © European Molecular Biology Organization 2008 Abstract The Wnt–PCP (planar cell polarity, PCP) pathway regulates cell polarity and convergent extension movements during axis formation in vertebrates by activation of Rho and Rac, leading to the re‐organization of the actin cytoskeleton. Rho and Rac activation require guanine nucleotide‐exchange factors (GEFs), but the identity of the GEF involved in Wnt–PCP‐mediated convergent extension is unknown. Here we report the identification of the weak‐similarity GEF (WGEF) gene by a microarray‐based screen for notochord enriched genes, and show that WGEF is involved in Wnt‐regulated convergent extension. Overexpression of WGEF activated RhoA and rescued the suppression of convergent extension by dominant‐negative Wnt‐11, whereas depletion of WGEF led to suppression of convergent extension that could be rescued by RhoA or Rho‐associated kinase activation. WGEF protein preferentially localized at the plasma membrane, and Frizzled‐7 induced colocalization of Dishevelled and WGEF. WGEF protein can bind to Dishevelled and Daam‐1, and deletion of the Dishevelled‐binding domain generates a hyperactive from of WGEF. These results indicate that WGEF is a component of the Wnt–PCP pathway that connects Dishevelled to Rho activation. convergent extension (dpeaa)DE-He213 gastrulation (dpeaa)DE-He213 GEF (dpeaa)DE-He213 Wnt–PCP (dpeaa)DE-He213 Zhao, Hui verfasserin aut Dawid, Igor B verfasserin aut Enthalten in The EMBO Journal Nature Publishing Group UK, 2023 27(2008), 4 vom: 07. Feb., Seite 606-617 (DE-627)266022529 (DE-600)1467419-1 1460-2075 nnns volume:27 year:2008 number:4 day:07 month:02 pages:606-617 https://dx.doi.org/10.1038/emboj.2008.9 X:SPRINGER Resolving-System lizenzpflichtig Volltext SYSFLAG_0 GBV_SPRINGER GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_72 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_161 GBV_ILN_168 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_252 GBV_ILN_266 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2018 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4029 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4155 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4393 GBV_ILN_4700 AR 27 2008 4 07 02 606-617 |
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10.1038/emboj.2008.9 doi (DE-627)SPR057860270 (SPR)emboj.2008.9-e DE-627 ger DE-627 rakwb eng Tanegashima, Kosuke verfasserin aut WGEF activates Rho in the Wnt–PCP pathway and controls convergent extension in Xenopus gastrulation 2008 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © European Molecular Biology Organization 2008 Abstract The Wnt–PCP (planar cell polarity, PCP) pathway regulates cell polarity and convergent extension movements during axis formation in vertebrates by activation of Rho and Rac, leading to the re‐organization of the actin cytoskeleton. Rho and Rac activation require guanine nucleotide‐exchange factors (GEFs), but the identity of the GEF involved in Wnt–PCP‐mediated convergent extension is unknown. Here we report the identification of the weak‐similarity GEF (WGEF) gene by a microarray‐based screen for notochord enriched genes, and show that WGEF is involved in Wnt‐regulated convergent extension. Overexpression of WGEF activated RhoA and rescued the suppression of convergent extension by dominant‐negative Wnt‐11, whereas depletion of WGEF led to suppression of convergent extension that could be rescued by RhoA or Rho‐associated kinase activation. WGEF protein preferentially localized at the plasma membrane, and Frizzled‐7 induced colocalization of Dishevelled and WGEF. WGEF protein can bind to Dishevelled and Daam‐1, and deletion of the Dishevelled‐binding domain generates a hyperactive from of WGEF. These results indicate that WGEF is a component of the Wnt–PCP pathway that connects Dishevelled to Rho activation. convergent extension (dpeaa)DE-He213 gastrulation (dpeaa)DE-He213 GEF (dpeaa)DE-He213 Wnt–PCP (dpeaa)DE-He213 Zhao, Hui verfasserin aut Dawid, Igor B verfasserin aut Enthalten in The EMBO Journal Nature Publishing Group UK, 2023 27(2008), 4 vom: 07. Feb., Seite 606-617 (DE-627)266022529 (DE-600)1467419-1 1460-2075 nnns volume:27 year:2008 number:4 day:07 month:02 pages:606-617 https://dx.doi.org/10.1038/emboj.2008.9 X:SPRINGER Resolving-System lizenzpflichtig Volltext SYSFLAG_0 GBV_SPRINGER GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_72 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_161 GBV_ILN_168 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_252 GBV_ILN_266 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2018 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4029 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4155 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4393 GBV_ILN_4700 AR 27 2008 4 07 02 606-617 |
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10.1038/emboj.2008.9 doi (DE-627)SPR057860270 (SPR)emboj.2008.9-e DE-627 ger DE-627 rakwb eng Tanegashima, Kosuke verfasserin aut WGEF activates Rho in the Wnt–PCP pathway and controls convergent extension in Xenopus gastrulation 2008 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © European Molecular Biology Organization 2008 Abstract The Wnt–PCP (planar cell polarity, PCP) pathway regulates cell polarity and convergent extension movements during axis formation in vertebrates by activation of Rho and Rac, leading to the re‐organization of the actin cytoskeleton. Rho and Rac activation require guanine nucleotide‐exchange factors (GEFs), but the identity of the GEF involved in Wnt–PCP‐mediated convergent extension is unknown. Here we report the identification of the weak‐similarity GEF (WGEF) gene by a microarray‐based screen for notochord enriched genes, and show that WGEF is involved in Wnt‐regulated convergent extension. Overexpression of WGEF activated RhoA and rescued the suppression of convergent extension by dominant‐negative Wnt‐11, whereas depletion of WGEF led to suppression of convergent extension that could be rescued by RhoA or Rho‐associated kinase activation. WGEF protein preferentially localized at the plasma membrane, and Frizzled‐7 induced colocalization of Dishevelled and WGEF. WGEF protein can bind to Dishevelled and Daam‐1, and deletion of the Dishevelled‐binding domain generates a hyperactive from of WGEF. These results indicate that WGEF is a component of the Wnt–PCP pathway that connects Dishevelled to Rho activation. convergent extension (dpeaa)DE-He213 gastrulation (dpeaa)DE-He213 GEF (dpeaa)DE-He213 Wnt–PCP (dpeaa)DE-He213 Zhao, Hui verfasserin aut Dawid, Igor B verfasserin aut Enthalten in The EMBO Journal Nature Publishing Group UK, 2023 27(2008), 4 vom: 07. Feb., Seite 606-617 (DE-627)266022529 (DE-600)1467419-1 1460-2075 nnns volume:27 year:2008 number:4 day:07 month:02 pages:606-617 https://dx.doi.org/10.1038/emboj.2008.9 X:SPRINGER Resolving-System lizenzpflichtig Volltext SYSFLAG_0 GBV_SPRINGER GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_72 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_161 GBV_ILN_168 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_252 GBV_ILN_266 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2018 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4029 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4155 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4393 GBV_ILN_4700 AR 27 2008 4 07 02 606-617 |
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Enthalten in The EMBO Journal 27(2008), 4 vom: 07. Feb., Seite 606-617 volume:27 year:2008 number:4 day:07 month:02 pages:606-617 |
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Enthalten in The EMBO Journal 27(2008), 4 vom: 07. Feb., Seite 606-617 volume:27 year:2008 number:4 day:07 month:02 pages:606-617 |
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Tanegashima, Kosuke @@aut@@ Zhao, Hui @@aut@@ Dawid, Igor B @@aut@@ |
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Tanegashima, Kosuke |
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Tanegashima, Kosuke misc convergent extension misc gastrulation misc GEF misc Wnt–PCP WGEF activates Rho in the Wnt–PCP pathway and controls convergent extension in Xenopus gastrulation |
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WGEF activates Rho in the Wnt–PCP pathway and controls convergent extension in Xenopus gastrulation convergent extension (dpeaa)DE-He213 gastrulation (dpeaa)DE-He213 GEF (dpeaa)DE-He213 Wnt–PCP (dpeaa)DE-He213 |
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WGEF activates Rho in the Wnt–PCP pathway and controls convergent extension in Xenopus gastrulation |
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WGEF activates Rho in the Wnt–PCP pathway and controls convergent extension in Xenopus gastrulation |
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wgef activates rho in the wnt–pcp pathway and controls convergent extension in xenopus gastrulation |
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WGEF activates Rho in the Wnt–PCP pathway and controls convergent extension in Xenopus gastrulation |
| abstract |
Abstract The Wnt–PCP (planar cell polarity, PCP) pathway regulates cell polarity and convergent extension movements during axis formation in vertebrates by activation of Rho and Rac, leading to the re‐organization of the actin cytoskeleton. Rho and Rac activation require guanine nucleotide‐exchange factors (GEFs), but the identity of the GEF involved in Wnt–PCP‐mediated convergent extension is unknown. Here we report the identification of the weak‐similarity GEF (WGEF) gene by a microarray‐based screen for notochord enriched genes, and show that WGEF is involved in Wnt‐regulated convergent extension. Overexpression of WGEF activated RhoA and rescued the suppression of convergent extension by dominant‐negative Wnt‐11, whereas depletion of WGEF led to suppression of convergent extension that could be rescued by RhoA or Rho‐associated kinase activation. WGEF protein preferentially localized at the plasma membrane, and Frizzled‐7 induced colocalization of Dishevelled and WGEF. WGEF protein can bind to Dishevelled and Daam‐1, and deletion of the Dishevelled‐binding domain generates a hyperactive from of WGEF. These results indicate that WGEF is a component of the Wnt–PCP pathway that connects Dishevelled to Rho activation. © European Molecular Biology Organization 2008 |
| abstractGer |
Abstract The Wnt–PCP (planar cell polarity, PCP) pathway regulates cell polarity and convergent extension movements during axis formation in vertebrates by activation of Rho and Rac, leading to the re‐organization of the actin cytoskeleton. Rho and Rac activation require guanine nucleotide‐exchange factors (GEFs), but the identity of the GEF involved in Wnt–PCP‐mediated convergent extension is unknown. Here we report the identification of the weak‐similarity GEF (WGEF) gene by a microarray‐based screen for notochord enriched genes, and show that WGEF is involved in Wnt‐regulated convergent extension. Overexpression of WGEF activated RhoA and rescued the suppression of convergent extension by dominant‐negative Wnt‐11, whereas depletion of WGEF led to suppression of convergent extension that could be rescued by RhoA or Rho‐associated kinase activation. WGEF protein preferentially localized at the plasma membrane, and Frizzled‐7 induced colocalization of Dishevelled and WGEF. WGEF protein can bind to Dishevelled and Daam‐1, and deletion of the Dishevelled‐binding domain generates a hyperactive from of WGEF. These results indicate that WGEF is a component of the Wnt–PCP pathway that connects Dishevelled to Rho activation. © European Molecular Biology Organization 2008 |
| abstract_unstemmed |
Abstract The Wnt–PCP (planar cell polarity, PCP) pathway regulates cell polarity and convergent extension movements during axis formation in vertebrates by activation of Rho and Rac, leading to the re‐organization of the actin cytoskeleton. Rho and Rac activation require guanine nucleotide‐exchange factors (GEFs), but the identity of the GEF involved in Wnt–PCP‐mediated convergent extension is unknown. Here we report the identification of the weak‐similarity GEF (WGEF) gene by a microarray‐based screen for notochord enriched genes, and show that WGEF is involved in Wnt‐regulated convergent extension. Overexpression of WGEF activated RhoA and rescued the suppression of convergent extension by dominant‐negative Wnt‐11, whereas depletion of WGEF led to suppression of convergent extension that could be rescued by RhoA or Rho‐associated kinase activation. WGEF protein preferentially localized at the plasma membrane, and Frizzled‐7 induced colocalization of Dishevelled and WGEF. WGEF protein can bind to Dishevelled and Daam‐1, and deletion of the Dishevelled‐binding domain generates a hyperactive from of WGEF. These results indicate that WGEF is a component of the Wnt–PCP pathway that connects Dishevelled to Rho activation. © European Molecular Biology Organization 2008 |
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WGEF activates Rho in the Wnt–PCP pathway and controls convergent extension in Xenopus gastrulation |
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