GT‐rich promoters can drive RNA pol II transcription and deposition of H2A.Z in African trypanosomes
Abstract Genome‐wide transcription studies are revealing an increasing number of “dispersed promoters” that, unlike “focused promoters”, lack well‐conserved sequence motifs and tight regulation. Dispersed promoters are nevertheless marked by well‐defined chromatin structures, suggesting that specifi...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
Wedel, Carolin [verfasserIn] Förstner, Konrad U [verfasserIn] Derr, Ramona [verfasserIn] Siegel, T Nicolai [verfasserIn] |
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| Format: |
E-Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
2017 |
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| Schlagwörter: |
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| Anmerkung: |
© The Author(s) 2017 |
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| Übergeordnetes Werk: |
Enthalten in: The EMBO Journal - Nature Publishing Group UK, 2023, 36(2017), 17 vom: 12. Juli, Seite 2581-2594 |
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| Übergeordnetes Werk: |
volume:36 ; year:2017 ; number:17 ; day:12 ; month:07 ; pages:2581-2594 |
| Links: |
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| DOI / URN: |
10.15252/embj.201695323 |
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| Katalog-ID: |
SPR05795674X |
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| 100 | 1 | |a Wedel, Carolin |e verfasserin |0 (orcid)0000-0001-6730-2220 |4 aut | |
| 245 | 1 | 0 | |a GT‐rich promoters can drive RNA pol II transcription and deposition of H2A.Z in African trypanosomes |
| 264 | 1 | |c 2017 | |
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| 520 | |a Abstract Genome‐wide transcription studies are revealing an increasing number of “dispersed promoters” that, unlike “focused promoters”, lack well‐conserved sequence motifs and tight regulation. Dispersed promoters are nevertheless marked by well‐defined chromatin structures, suggesting that specific sequence elements must exist in these unregulated promoters. Here, we have analyzed regions of transcription initiation in the eukaryotic parasite Trypanosoma brucei, in which RNA polymerase II transcription initiation occurs over broad regions without distinct promoter motifs and lacks regulation. Using a combination of site‐specific and genome‐wide assays, we identified GT‐rich promoters that can drive transcription and promote the targeted deposition of the histone variant H2A.Z in a genomic context‐dependent manner. In addition, upon mapping nucleosome occupancy at high resolution, we find nucleosome positioning to correlate with RNA pol II enrichment and gene expression, pointing to a role in RNA maturation. Nucleosome positioning may thus represent a previously unrecognized layer of gene regulation in trypanosomes. Our findings show that even highly dispersed, unregulated promoters contain specific DNA elements that are able to induce transcription and changes in chromatin structure. | ||
| 520 | |a Synopsis The lack of recognisable promoters in trypanosomes has limited our understanding of transcriptional control in this important pathogen. Via genome‐wide assays this study shows that transcription initiates broadly on GT‐rich elements that control incorporation of activating histone variants. RNA pol II transcription initiates across a 2 kb‐wide region upstream of polycistronic transcription units.GT‐rich sequence elements enriched at transcription start sites can induce transcription and recruit the histone variant H2A.Z.Sites enriched in H2A.Z show increased sensitivity to MNase.Nucleosome occupancy upstream of genes correlates with RNA pol II enrichment and transcript levels.Composition of polyY tract affects nucleosome positioning and transcript levels. | ||
| 520 | |a Graphical Abstract While trypanosomes lack classical promoter elements, they initiate transcription broadly over GT‐rich sequences that control incorporation of activating histone variants. | ||
| 650 | 4 | |a core promoter |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a histone variant |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a nucleosome occupancy |7 (dpeaa)DE-He213 | |
| 700 | 1 | |a Förstner, Konrad U |e verfasserin |0 (orcid)0000-0002-1481-2996 |4 aut | |
| 700 | 1 | |a Derr, Ramona |e verfasserin |4 aut | |
| 700 | 1 | |a Siegel, T Nicolai |e verfasserin |0 (orcid)0000-0002-1715-7907 |4 aut | |
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10.15252/embj.201695323 doi (DE-627)SPR05795674X (SPR)embj.201695323-e DE-627 ger DE-627 rakwb eng Wedel, Carolin verfasserin (orcid)0000-0001-6730-2220 aut GT‐rich promoters can drive RNA pol II transcription and deposition of H2A.Z in African trypanosomes 2017 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s) 2017 Abstract Genome‐wide transcription studies are revealing an increasing number of “dispersed promoters” that, unlike “focused promoters”, lack well‐conserved sequence motifs and tight regulation. Dispersed promoters are nevertheless marked by well‐defined chromatin structures, suggesting that specific sequence elements must exist in these unregulated promoters. Here, we have analyzed regions of transcription initiation in the eukaryotic parasite Trypanosoma brucei, in which RNA polymerase II transcription initiation occurs over broad regions without distinct promoter motifs and lacks regulation. Using a combination of site‐specific and genome‐wide assays, we identified GT‐rich promoters that can drive transcription and promote the targeted deposition of the histone variant H2A.Z in a genomic context‐dependent manner. In addition, upon mapping nucleosome occupancy at high resolution, we find nucleosome positioning to correlate with RNA pol II enrichment and gene expression, pointing to a role in RNA maturation. Nucleosome positioning may thus represent a previously unrecognized layer of gene regulation in trypanosomes. Our findings show that even highly dispersed, unregulated promoters contain specific DNA elements that are able to induce transcription and changes in chromatin structure. Synopsis The lack of recognisable promoters in trypanosomes has limited our understanding of transcriptional control in this important pathogen. Via genome‐wide assays this study shows that transcription initiates broadly on GT‐rich elements that control incorporation of activating histone variants. RNA pol II transcription initiates across a 2 kb‐wide region upstream of polycistronic transcription units.GT‐rich sequence elements enriched at transcription start sites can induce transcription and recruit the histone variant H2A.Z.Sites enriched in H2A.Z show increased sensitivity to MNase.Nucleosome occupancy upstream of genes correlates with RNA pol II enrichment and transcript levels.Composition of polyY tract affects nucleosome positioning and transcript levels. Graphical Abstract While trypanosomes lack classical promoter elements, they initiate transcription broadly over GT‐rich sequences that control incorporation of activating histone variants. core promoter (dpeaa)DE-He213 histone variant (dpeaa)DE-He213 nucleosome occupancy (dpeaa)DE-He213 Förstner, Konrad U verfasserin (orcid)0000-0002-1481-2996 aut Derr, Ramona verfasserin aut Siegel, T Nicolai verfasserin (orcid)0000-0002-1715-7907 aut Enthalten in The EMBO Journal Nature Publishing Group UK, 2023 36(2017), 17 vom: 12. Juli, Seite 2581-2594 (DE-627)266022529 (DE-600)1467419-1 1460-2075 nnns volume:36 year:2017 number:17 day:12 month:07 pages:2581-2594 https://dx.doi.org/10.15252/embj.201695323 X:SPRINGER Resolving-System kostenfrei Volltext SYSFLAG_0 GBV_SPRINGER GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_72 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_161 GBV_ILN_168 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_211 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_252 GBV_ILN_266 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2018 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4029 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4116 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4155 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4311 GBV_ILN_4313 GBV_ILN_4314 GBV_ILN_4318 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4393 GBV_ILN_4598 GBV_ILN_4700 AR 36 2017 17 12 07 2581-2594 |
| spelling |
10.15252/embj.201695323 doi (DE-627)SPR05795674X (SPR)embj.201695323-e DE-627 ger DE-627 rakwb eng Wedel, Carolin verfasserin (orcid)0000-0001-6730-2220 aut GT‐rich promoters can drive RNA pol II transcription and deposition of H2A.Z in African trypanosomes 2017 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s) 2017 Abstract Genome‐wide transcription studies are revealing an increasing number of “dispersed promoters” that, unlike “focused promoters”, lack well‐conserved sequence motifs and tight regulation. Dispersed promoters are nevertheless marked by well‐defined chromatin structures, suggesting that specific sequence elements must exist in these unregulated promoters. Here, we have analyzed regions of transcription initiation in the eukaryotic parasite Trypanosoma brucei, in which RNA polymerase II transcription initiation occurs over broad regions without distinct promoter motifs and lacks regulation. Using a combination of site‐specific and genome‐wide assays, we identified GT‐rich promoters that can drive transcription and promote the targeted deposition of the histone variant H2A.Z in a genomic context‐dependent manner. In addition, upon mapping nucleosome occupancy at high resolution, we find nucleosome positioning to correlate with RNA pol II enrichment and gene expression, pointing to a role in RNA maturation. Nucleosome positioning may thus represent a previously unrecognized layer of gene regulation in trypanosomes. Our findings show that even highly dispersed, unregulated promoters contain specific DNA elements that are able to induce transcription and changes in chromatin structure. Synopsis The lack of recognisable promoters in trypanosomes has limited our understanding of transcriptional control in this important pathogen. Via genome‐wide assays this study shows that transcription initiates broadly on GT‐rich elements that control incorporation of activating histone variants. RNA pol II transcription initiates across a 2 kb‐wide region upstream of polycistronic transcription units.GT‐rich sequence elements enriched at transcription start sites can induce transcription and recruit the histone variant H2A.Z.Sites enriched in H2A.Z show increased sensitivity to MNase.Nucleosome occupancy upstream of genes correlates with RNA pol II enrichment and transcript levels.Composition of polyY tract affects nucleosome positioning and transcript levels. Graphical Abstract While trypanosomes lack classical promoter elements, they initiate transcription broadly over GT‐rich sequences that control incorporation of activating histone variants. core promoter (dpeaa)DE-He213 histone variant (dpeaa)DE-He213 nucleosome occupancy (dpeaa)DE-He213 Förstner, Konrad U verfasserin (orcid)0000-0002-1481-2996 aut Derr, Ramona verfasserin aut Siegel, T Nicolai verfasserin (orcid)0000-0002-1715-7907 aut Enthalten in The EMBO Journal Nature Publishing Group UK, 2023 36(2017), 17 vom: 12. Juli, Seite 2581-2594 (DE-627)266022529 (DE-600)1467419-1 1460-2075 nnns volume:36 year:2017 number:17 day:12 month:07 pages:2581-2594 https://dx.doi.org/10.15252/embj.201695323 X:SPRINGER Resolving-System kostenfrei Volltext SYSFLAG_0 GBV_SPRINGER GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_72 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_161 GBV_ILN_168 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_211 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_252 GBV_ILN_266 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2018 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4029 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4116 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4155 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4311 GBV_ILN_4313 GBV_ILN_4314 GBV_ILN_4318 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4393 GBV_ILN_4598 GBV_ILN_4700 AR 36 2017 17 12 07 2581-2594 |
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10.15252/embj.201695323 doi (DE-627)SPR05795674X (SPR)embj.201695323-e DE-627 ger DE-627 rakwb eng Wedel, Carolin verfasserin (orcid)0000-0001-6730-2220 aut GT‐rich promoters can drive RNA pol II transcription and deposition of H2A.Z in African trypanosomes 2017 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s) 2017 Abstract Genome‐wide transcription studies are revealing an increasing number of “dispersed promoters” that, unlike “focused promoters”, lack well‐conserved sequence motifs and tight regulation. Dispersed promoters are nevertheless marked by well‐defined chromatin structures, suggesting that specific sequence elements must exist in these unregulated promoters. Here, we have analyzed regions of transcription initiation in the eukaryotic parasite Trypanosoma brucei, in which RNA polymerase II transcription initiation occurs over broad regions without distinct promoter motifs and lacks regulation. Using a combination of site‐specific and genome‐wide assays, we identified GT‐rich promoters that can drive transcription and promote the targeted deposition of the histone variant H2A.Z in a genomic context‐dependent manner. In addition, upon mapping nucleosome occupancy at high resolution, we find nucleosome positioning to correlate with RNA pol II enrichment and gene expression, pointing to a role in RNA maturation. Nucleosome positioning may thus represent a previously unrecognized layer of gene regulation in trypanosomes. Our findings show that even highly dispersed, unregulated promoters contain specific DNA elements that are able to induce transcription and changes in chromatin structure. Synopsis The lack of recognisable promoters in trypanosomes has limited our understanding of transcriptional control in this important pathogen. Via genome‐wide assays this study shows that transcription initiates broadly on GT‐rich elements that control incorporation of activating histone variants. RNA pol II transcription initiates across a 2 kb‐wide region upstream of polycistronic transcription units.GT‐rich sequence elements enriched at transcription start sites can induce transcription and recruit the histone variant H2A.Z.Sites enriched in H2A.Z show increased sensitivity to MNase.Nucleosome occupancy upstream of genes correlates with RNA pol II enrichment and transcript levels.Composition of polyY tract affects nucleosome positioning and transcript levels. Graphical Abstract While trypanosomes lack classical promoter elements, they initiate transcription broadly over GT‐rich sequences that control incorporation of activating histone variants. core promoter (dpeaa)DE-He213 histone variant (dpeaa)DE-He213 nucleosome occupancy (dpeaa)DE-He213 Förstner, Konrad U verfasserin (orcid)0000-0002-1481-2996 aut Derr, Ramona verfasserin aut Siegel, T Nicolai verfasserin (orcid)0000-0002-1715-7907 aut Enthalten in The EMBO Journal Nature Publishing Group UK, 2023 36(2017), 17 vom: 12. Juli, Seite 2581-2594 (DE-627)266022529 (DE-600)1467419-1 1460-2075 nnns volume:36 year:2017 number:17 day:12 month:07 pages:2581-2594 https://dx.doi.org/10.15252/embj.201695323 X:SPRINGER Resolving-System kostenfrei Volltext SYSFLAG_0 GBV_SPRINGER GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_72 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_161 GBV_ILN_168 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_211 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_252 GBV_ILN_266 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2018 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4029 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4116 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4155 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4311 GBV_ILN_4313 GBV_ILN_4314 GBV_ILN_4318 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4393 GBV_ILN_4598 GBV_ILN_4700 AR 36 2017 17 12 07 2581-2594 |
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10.15252/embj.201695323 doi (DE-627)SPR05795674X (SPR)embj.201695323-e DE-627 ger DE-627 rakwb eng Wedel, Carolin verfasserin (orcid)0000-0001-6730-2220 aut GT‐rich promoters can drive RNA pol II transcription and deposition of H2A.Z in African trypanosomes 2017 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s) 2017 Abstract Genome‐wide transcription studies are revealing an increasing number of “dispersed promoters” that, unlike “focused promoters”, lack well‐conserved sequence motifs and tight regulation. Dispersed promoters are nevertheless marked by well‐defined chromatin structures, suggesting that specific sequence elements must exist in these unregulated promoters. Here, we have analyzed regions of transcription initiation in the eukaryotic parasite Trypanosoma brucei, in which RNA polymerase II transcription initiation occurs over broad regions without distinct promoter motifs and lacks regulation. Using a combination of site‐specific and genome‐wide assays, we identified GT‐rich promoters that can drive transcription and promote the targeted deposition of the histone variant H2A.Z in a genomic context‐dependent manner. In addition, upon mapping nucleosome occupancy at high resolution, we find nucleosome positioning to correlate with RNA pol II enrichment and gene expression, pointing to a role in RNA maturation. Nucleosome positioning may thus represent a previously unrecognized layer of gene regulation in trypanosomes. Our findings show that even highly dispersed, unregulated promoters contain specific DNA elements that are able to induce transcription and changes in chromatin structure. Synopsis The lack of recognisable promoters in trypanosomes has limited our understanding of transcriptional control in this important pathogen. Via genome‐wide assays this study shows that transcription initiates broadly on GT‐rich elements that control incorporation of activating histone variants. RNA pol II transcription initiates across a 2 kb‐wide region upstream of polycistronic transcription units.GT‐rich sequence elements enriched at transcription start sites can induce transcription and recruit the histone variant H2A.Z.Sites enriched in H2A.Z show increased sensitivity to MNase.Nucleosome occupancy upstream of genes correlates with RNA pol II enrichment and transcript levels.Composition of polyY tract affects nucleosome positioning and transcript levels. Graphical Abstract While trypanosomes lack classical promoter elements, they initiate transcription broadly over GT‐rich sequences that control incorporation of activating histone variants. core promoter (dpeaa)DE-He213 histone variant (dpeaa)DE-He213 nucleosome occupancy (dpeaa)DE-He213 Förstner, Konrad U verfasserin (orcid)0000-0002-1481-2996 aut Derr, Ramona verfasserin aut Siegel, T Nicolai verfasserin (orcid)0000-0002-1715-7907 aut Enthalten in The EMBO Journal Nature Publishing Group UK, 2023 36(2017), 17 vom: 12. Juli, Seite 2581-2594 (DE-627)266022529 (DE-600)1467419-1 1460-2075 nnns volume:36 year:2017 number:17 day:12 month:07 pages:2581-2594 https://dx.doi.org/10.15252/embj.201695323 X:SPRINGER Resolving-System kostenfrei Volltext SYSFLAG_0 GBV_SPRINGER GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_72 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_161 GBV_ILN_168 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_211 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_252 GBV_ILN_266 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2018 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4029 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4116 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4155 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4311 GBV_ILN_4313 GBV_ILN_4314 GBV_ILN_4318 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4393 GBV_ILN_4598 GBV_ILN_4700 AR 36 2017 17 12 07 2581-2594 |
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10.15252/embj.201695323 doi (DE-627)SPR05795674X (SPR)embj.201695323-e DE-627 ger DE-627 rakwb eng Wedel, Carolin verfasserin (orcid)0000-0001-6730-2220 aut GT‐rich promoters can drive RNA pol II transcription and deposition of H2A.Z in African trypanosomes 2017 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s) 2017 Abstract Genome‐wide transcription studies are revealing an increasing number of “dispersed promoters” that, unlike “focused promoters”, lack well‐conserved sequence motifs and tight regulation. Dispersed promoters are nevertheless marked by well‐defined chromatin structures, suggesting that specific sequence elements must exist in these unregulated promoters. Here, we have analyzed regions of transcription initiation in the eukaryotic parasite Trypanosoma brucei, in which RNA polymerase II transcription initiation occurs over broad regions without distinct promoter motifs and lacks regulation. Using a combination of site‐specific and genome‐wide assays, we identified GT‐rich promoters that can drive transcription and promote the targeted deposition of the histone variant H2A.Z in a genomic context‐dependent manner. In addition, upon mapping nucleosome occupancy at high resolution, we find nucleosome positioning to correlate with RNA pol II enrichment and gene expression, pointing to a role in RNA maturation. Nucleosome positioning may thus represent a previously unrecognized layer of gene regulation in trypanosomes. Our findings show that even highly dispersed, unregulated promoters contain specific DNA elements that are able to induce transcription and changes in chromatin structure. Synopsis The lack of recognisable promoters in trypanosomes has limited our understanding of transcriptional control in this important pathogen. Via genome‐wide assays this study shows that transcription initiates broadly on GT‐rich elements that control incorporation of activating histone variants. RNA pol II transcription initiates across a 2 kb‐wide region upstream of polycistronic transcription units.GT‐rich sequence elements enriched at transcription start sites can induce transcription and recruit the histone variant H2A.Z.Sites enriched in H2A.Z show increased sensitivity to MNase.Nucleosome occupancy upstream of genes correlates with RNA pol II enrichment and transcript levels.Composition of polyY tract affects nucleosome positioning and transcript levels. Graphical Abstract While trypanosomes lack classical promoter elements, they initiate transcription broadly over GT‐rich sequences that control incorporation of activating histone variants. core promoter (dpeaa)DE-He213 histone variant (dpeaa)DE-He213 nucleosome occupancy (dpeaa)DE-He213 Förstner, Konrad U verfasserin (orcid)0000-0002-1481-2996 aut Derr, Ramona verfasserin aut Siegel, T Nicolai verfasserin (orcid)0000-0002-1715-7907 aut Enthalten in The EMBO Journal Nature Publishing Group UK, 2023 36(2017), 17 vom: 12. Juli, Seite 2581-2594 (DE-627)266022529 (DE-600)1467419-1 1460-2075 nnns volume:36 year:2017 number:17 day:12 month:07 pages:2581-2594 https://dx.doi.org/10.15252/embj.201695323 X:SPRINGER Resolving-System kostenfrei Volltext SYSFLAG_0 GBV_SPRINGER GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_72 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_161 GBV_ILN_168 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_211 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_252 GBV_ILN_266 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2018 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4029 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4116 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4155 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4311 GBV_ILN_4313 GBV_ILN_4314 GBV_ILN_4318 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4393 GBV_ILN_4598 GBV_ILN_4700 AR 36 2017 17 12 07 2581-2594 |
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Enthalten in The EMBO Journal 36(2017), 17 vom: 12. Juli, Seite 2581-2594 volume:36 year:2017 number:17 day:12 month:07 pages:2581-2594 |
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Wedel, Carolin @@aut@@ Förstner, Konrad U @@aut@@ Derr, Ramona @@aut@@ Siegel, T Nicolai @@aut@@ |
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<?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>01000naa a22002652 4500</leader><controlfield tag="001">SPR05795674X</controlfield><controlfield tag="003">DE-627</controlfield><controlfield tag="005">20241022064926.0</controlfield><controlfield tag="007">cr uuu---uuuuu</controlfield><controlfield tag="008">241022s2017 xx |||||o 00| ||eng c</controlfield><datafield tag="024" ind1="7" ind2=" "><subfield code="a">10.15252/embj.201695323</subfield><subfield code="2">doi</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-627)SPR05795674X</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(SPR)embj.201695323-e</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-627</subfield><subfield code="b">ger</subfield><subfield code="c">DE-627</subfield><subfield code="e">rakwb</subfield></datafield><datafield tag="041" ind1=" " ind2=" "><subfield code="a">eng</subfield></datafield><datafield tag="100" ind1="1" ind2=" "><subfield code="a">Wedel, Carolin</subfield><subfield code="e">verfasserin</subfield><subfield code="0">(orcid)0000-0001-6730-2220</subfield><subfield code="4">aut</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">GT‐rich promoters can drive RNA pol II transcription and deposition of H2A.Z in African trypanosomes</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="c">2017</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="a">Text</subfield><subfield code="b">txt</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="a">Computermedien</subfield><subfield code="b">c</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="a">Online-Ressource</subfield><subfield code="b">cr</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="500" ind1=" " ind2=" "><subfield code="a">© The Author(s) 2017</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a">Abstract Genome‐wide transcription studies are revealing an increasing number of “dispersed promoters” that, unlike “focused promoters”, lack well‐conserved sequence motifs and tight regulation. Dispersed promoters are nevertheless marked by well‐defined chromatin structures, suggesting that specific sequence elements must exist in these unregulated promoters. Here, we have analyzed regions of transcription initiation in the eukaryotic parasite Trypanosoma brucei, in which RNA polymerase II transcription initiation occurs over broad regions without distinct promoter motifs and lacks regulation. Using a combination of site‐specific and genome‐wide assays, we identified GT‐rich promoters that can drive transcription and promote the targeted deposition of the histone variant H2A.Z in a genomic context‐dependent manner. In addition, upon mapping nucleosome occupancy at high resolution, we find nucleosome positioning to correlate with RNA pol II enrichment and gene expression, pointing to a role in RNA maturation. Nucleosome positioning may thus represent a previously unrecognized layer of gene regulation in trypanosomes. Our findings show that even highly dispersed, unregulated promoters contain specific DNA elements that are able to induce transcription and changes in chromatin structure.</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a">Synopsis The lack of recognisable promoters in trypanosomes has limited our understanding of transcriptional control in this important pathogen. Via genome‐wide assays this study shows that transcription initiates broadly on GT‐rich elements that control incorporation of activating histone variants. RNA pol II transcription initiates across a 2 kb‐wide region upstream of polycistronic transcription units.GT‐rich sequence elements enriched at transcription start sites can induce transcription and recruit the histone variant H2A.Z.Sites enriched in H2A.Z show increased sensitivity to MNase.Nucleosome occupancy upstream of genes correlates with RNA pol II enrichment and transcript levels.Composition of polyY tract affects nucleosome positioning and transcript levels.</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a">Graphical Abstract While trypanosomes lack classical promoter elements, they initiate transcription broadly over GT‐rich sequences that control incorporation of activating histone variants.</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">core promoter</subfield><subfield code="7">(dpeaa)DE-He213</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">histone variant</subfield><subfield code="7">(dpeaa)DE-He213</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">nucleosome occupancy</subfield><subfield code="7">(dpeaa)DE-He213</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Förstner, Konrad U</subfield><subfield code="e">verfasserin</subfield><subfield code="0">(orcid)0000-0002-1481-2996</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Derr, Ramona</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Siegel, T Nicolai</subfield><subfield code="e">verfasserin</subfield><subfield code="0">(orcid)0000-0002-1715-7907</subfield><subfield code="4">aut</subfield></datafield><datafield tag="773" ind1="0" ind2="8"><subfield code="i">Enthalten in</subfield><subfield code="t">The EMBO Journal</subfield><subfield code="d">Nature Publishing Group UK, 2023</subfield><subfield code="g">36(2017), 17 vom: 12. Juli, Seite 2581-2594</subfield><subfield code="w">(DE-627)266022529</subfield><subfield code="w">(DE-600)1467419-1</subfield><subfield code="x">1460-2075</subfield><subfield code="7">nnns</subfield></datafield><datafield tag="773" ind1="1" ind2="8"><subfield code="g">volume:36</subfield><subfield code="g">year:2017</subfield><subfield code="g">number:17</subfield><subfield code="g">day:12</subfield><subfield code="g">month:07</subfield><subfield code="g">pages:2581-2594</subfield></datafield><datafield tag="856" ind1="4" ind2="0"><subfield code="u">https://dx.doi.org/10.15252/embj.201695323</subfield><subfield code="m">X:SPRINGER</subfield><subfield code="x">Resolving-System</subfield><subfield code="z">kostenfrei</subfield><subfield code="3">Volltext</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">SYSFLAG_0</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_SPRINGER</subfield></datafield><datafield tag="912" ind1=" " 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|
| author |
Wedel, Carolin |
| spellingShingle |
Wedel, Carolin misc core promoter misc histone variant misc nucleosome occupancy GT‐rich promoters can drive RNA pol II transcription and deposition of H2A.Z in African trypanosomes |
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Wedel, Carolin |
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1460-2075 |
| topic_title |
GT‐rich promoters can drive RNA pol II transcription and deposition of H2A.Z in African trypanosomes core promoter (dpeaa)DE-He213 histone variant (dpeaa)DE-He213 nucleosome occupancy (dpeaa)DE-He213 |
| topic |
misc core promoter misc histone variant misc nucleosome occupancy |
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misc core promoter misc histone variant misc nucleosome occupancy |
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misc core promoter misc histone variant misc nucleosome occupancy |
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Elektronische Aufsätze Aufsätze Elektronische Ressource |
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GT‐rich promoters can drive RNA pol II transcription and deposition of H2A.Z in African trypanosomes |
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(DE-627)SPR05795674X (SPR)embj.201695323-e |
| title_full |
GT‐rich promoters can drive RNA pol II transcription and deposition of H2A.Z in African trypanosomes |
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Wedel, Carolin |
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The EMBO Journal |
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The EMBO Journal |
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2017 |
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Wedel, Carolin Förstner, Konrad U Derr, Ramona Siegel, T Nicolai |
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Elektronische Aufsätze |
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Wedel, Carolin |
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10.15252/embj.201695323 |
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verfasserin |
| title_sort |
gt‐rich promoters can drive rna pol ii transcription and deposition of h2a.z in african trypanosomes |
| title_auth |
GT‐rich promoters can drive RNA pol II transcription and deposition of H2A.Z in African trypanosomes |
| abstract |
Abstract Genome‐wide transcription studies are revealing an increasing number of “dispersed promoters” that, unlike “focused promoters”, lack well‐conserved sequence motifs and tight regulation. Dispersed promoters are nevertheless marked by well‐defined chromatin structures, suggesting that specific sequence elements must exist in these unregulated promoters. Here, we have analyzed regions of transcription initiation in the eukaryotic parasite Trypanosoma brucei, in which RNA polymerase II transcription initiation occurs over broad regions without distinct promoter motifs and lacks regulation. Using a combination of site‐specific and genome‐wide assays, we identified GT‐rich promoters that can drive transcription and promote the targeted deposition of the histone variant H2A.Z in a genomic context‐dependent manner. In addition, upon mapping nucleosome occupancy at high resolution, we find nucleosome positioning to correlate with RNA pol II enrichment and gene expression, pointing to a role in RNA maturation. Nucleosome positioning may thus represent a previously unrecognized layer of gene regulation in trypanosomes. Our findings show that even highly dispersed, unregulated promoters contain specific DNA elements that are able to induce transcription and changes in chromatin structure. Synopsis The lack of recognisable promoters in trypanosomes has limited our understanding of transcriptional control in this important pathogen. Via genome‐wide assays this study shows that transcription initiates broadly on GT‐rich elements that control incorporation of activating histone variants. RNA pol II transcription initiates across a 2 kb‐wide region upstream of polycistronic transcription units.GT‐rich sequence elements enriched at transcription start sites can induce transcription and recruit the histone variant H2A.Z.Sites enriched in H2A.Z show increased sensitivity to MNase.Nucleosome occupancy upstream of genes correlates with RNA pol II enrichment and transcript levels.Composition of polyY tract affects nucleosome positioning and transcript levels. Graphical Abstract While trypanosomes lack classical promoter elements, they initiate transcription broadly over GT‐rich sequences that control incorporation of activating histone variants. © The Author(s) 2017 |
| abstractGer |
Abstract Genome‐wide transcription studies are revealing an increasing number of “dispersed promoters” that, unlike “focused promoters”, lack well‐conserved sequence motifs and tight regulation. Dispersed promoters are nevertheless marked by well‐defined chromatin structures, suggesting that specific sequence elements must exist in these unregulated promoters. Here, we have analyzed regions of transcription initiation in the eukaryotic parasite Trypanosoma brucei, in which RNA polymerase II transcription initiation occurs over broad regions without distinct promoter motifs and lacks regulation. Using a combination of site‐specific and genome‐wide assays, we identified GT‐rich promoters that can drive transcription and promote the targeted deposition of the histone variant H2A.Z in a genomic context‐dependent manner. In addition, upon mapping nucleosome occupancy at high resolution, we find nucleosome positioning to correlate with RNA pol II enrichment and gene expression, pointing to a role in RNA maturation. Nucleosome positioning may thus represent a previously unrecognized layer of gene regulation in trypanosomes. Our findings show that even highly dispersed, unregulated promoters contain specific DNA elements that are able to induce transcription and changes in chromatin structure. Synopsis The lack of recognisable promoters in trypanosomes has limited our understanding of transcriptional control in this important pathogen. Via genome‐wide assays this study shows that transcription initiates broadly on GT‐rich elements that control incorporation of activating histone variants. RNA pol II transcription initiates across a 2 kb‐wide region upstream of polycistronic transcription units.GT‐rich sequence elements enriched at transcription start sites can induce transcription and recruit the histone variant H2A.Z.Sites enriched in H2A.Z show increased sensitivity to MNase.Nucleosome occupancy upstream of genes correlates with RNA pol II enrichment and transcript levels.Composition of polyY tract affects nucleosome positioning and transcript levels. Graphical Abstract While trypanosomes lack classical promoter elements, they initiate transcription broadly over GT‐rich sequences that control incorporation of activating histone variants. © The Author(s) 2017 |
| abstract_unstemmed |
Abstract Genome‐wide transcription studies are revealing an increasing number of “dispersed promoters” that, unlike “focused promoters”, lack well‐conserved sequence motifs and tight regulation. Dispersed promoters are nevertheless marked by well‐defined chromatin structures, suggesting that specific sequence elements must exist in these unregulated promoters. Here, we have analyzed regions of transcription initiation in the eukaryotic parasite Trypanosoma brucei, in which RNA polymerase II transcription initiation occurs over broad regions without distinct promoter motifs and lacks regulation. Using a combination of site‐specific and genome‐wide assays, we identified GT‐rich promoters that can drive transcription and promote the targeted deposition of the histone variant H2A.Z in a genomic context‐dependent manner. In addition, upon mapping nucleosome occupancy at high resolution, we find nucleosome positioning to correlate with RNA pol II enrichment and gene expression, pointing to a role in RNA maturation. Nucleosome positioning may thus represent a previously unrecognized layer of gene regulation in trypanosomes. Our findings show that even highly dispersed, unregulated promoters contain specific DNA elements that are able to induce transcription and changes in chromatin structure. Synopsis The lack of recognisable promoters in trypanosomes has limited our understanding of transcriptional control in this important pathogen. Via genome‐wide assays this study shows that transcription initiates broadly on GT‐rich elements that control incorporation of activating histone variants. RNA pol II transcription initiates across a 2 kb‐wide region upstream of polycistronic transcription units.GT‐rich sequence elements enriched at transcription start sites can induce transcription and recruit the histone variant H2A.Z.Sites enriched in H2A.Z show increased sensitivity to MNase.Nucleosome occupancy upstream of genes correlates with RNA pol II enrichment and transcript levels.Composition of polyY tract affects nucleosome positioning and transcript levels. Graphical Abstract While trypanosomes lack classical promoter elements, they initiate transcription broadly over GT‐rich sequences that control incorporation of activating histone variants. © The Author(s) 2017 |
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| title_short |
GT‐rich promoters can drive RNA pol II transcription and deposition of H2A.Z in African trypanosomes |
| url |
https://dx.doi.org/10.15252/embj.201695323 |
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Förstner, Konrad U Derr, Ramona Siegel, T Nicolai |
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| doi_str |
10.15252/embj.201695323 |
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2024-10-22T04:51:55.052Z |
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|
| score |
7.4012003 |