Vesicle exocytosis stimulated by α‐latrotoxin is mediated by latrophilin and requires both external and stored $ Ca^{2+} $
Abstract α‐Latrotoxin (LTX) stimulates massive neurotransmitter release by two mechanisms: $ Ca^{2+} $‐dependent and ‐independent. Our studies on norepinephrine secretion from nerve terminals now reveal the different molecular basis of these two actions. The $ Ca^{2+} $‐dependent LTX‐evoked vesicle...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
Davletov, Bazbek A. [verfasserIn] Meunier, Frédéric A. [verfasserIn] Ashton, Anthony C. [verfasserIn] Matsushita, Hiroaki [verfasserIn] Hirst, Warren D. [verfasserIn] Lelianova, Vera G. [verfasserIn] Wilkin, Graham P. [verfasserIn] Dolly, J.Oliver [verfasserIn] Ushkaryov, Yuri A. [verfasserIn] |
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| Format: |
E-Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
1998 |
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| Schlagwörter: |
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| Anmerkung: |
© European Molecular Biology Organization 1998 |
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| Übergeordnetes Werk: |
Enthalten in: The EMBO Journal - Nature Publishing Group UK, 2023, 17(1998), 14 vom: 15. Juli, Seite 3909-3920 |
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| Übergeordnetes Werk: |
volume:17 ; year:1998 ; number:14 ; day:15 ; month:07 ; pages:3909-3920 |
| Links: |
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| DOI / URN: |
10.1093/emboj/17.14.3909 |
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| Katalog-ID: |
SPR058000623 |
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| 100 | 1 | |a Davletov, Bazbek A. |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Vesicle exocytosis stimulated by α‐latrotoxin is mediated by latrophilin and requires both external and stored $ Ca^{2+} $ |
| 264 | 1 | |c 1998 | |
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| 520 | |a Abstract α‐Latrotoxin (LTX) stimulates massive neurotransmitter release by two mechanisms: $ Ca^{2+} $‐dependent and ‐independent. Our studies on norepinephrine secretion from nerve terminals now reveal the different molecular basis of these two actions. The $ Ca^{2+} $‐dependent LTX‐evoked vesicle exocytosis (abolished by botulinum neurotoxins) is 10‐fold more sensitive to external $ Ca^{2+} $ than secretion triggered by depolarization or A23187; it does not, however, depend on the cation entry into terminals but requires intracellular $ Ca^{2+} $ and is blocked by drugs depleting $ Ca^{2+} $ stores and by inhibitors of phospholipase C (PLC). These data, together with binding studies, prove that latrophilin, which is linked to G proteins and inositol polyphosphate production, is the major functional LTX receptor. The $ Ca^{2+} $‐independent LTX‐stimulated release is not inhibited by botulinum neurotoxins or drugs interfering with $ Ca^{2+} $ metabolism and occurs via pores in the presynaptic membrane, large enough to allow efflux of neurotransmitters and other small molecules from the cytoplasm. Our results unite previously contradictory data about the toxin's effects and suggest that LTX‐stimulated exocytosis depends upon the co‐operative action of external and intracellular $ Ca^{2+} $ involving G proteins and PLC, whereas the $ Ca^{2+} $‐independent release is largely non‐vesicular. | ||
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| 650 | 4 | |a α‐latrotoxin |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a norepinephrine release |7 (dpeaa)DE-He213 | |
| 700 | 1 | |a Meunier, Frédéric A. |e verfasserin |4 aut | |
| 700 | 1 | |a Ashton, Anthony C. |e verfasserin |4 aut | |
| 700 | 1 | |a Matsushita, Hiroaki |e verfasserin |4 aut | |
| 700 | 1 | |a Hirst, Warren D. |e verfasserin |4 aut | |
| 700 | 1 | |a Lelianova, Vera G. |e verfasserin |4 aut | |
| 700 | 1 | |a Wilkin, Graham P. |e verfasserin |4 aut | |
| 700 | 1 | |a Dolly, J.Oliver |e verfasserin |4 aut | |
| 700 | 1 | |a Ushkaryov, Yuri A. |e verfasserin |4 aut | |
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1998 |
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1998 |
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10.1093/emboj/17.14.3909 doi (DE-627)SPR058000623 (SPR)17.14.3909-e DE-627 ger DE-627 rakwb eng Davletov, Bazbek A. verfasserin aut Vesicle exocytosis stimulated by α‐latrotoxin is mediated by latrophilin and requires both external and stored $ Ca^{2+} $ 1998 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © European Molecular Biology Organization 1998 Abstract α‐Latrotoxin (LTX) stimulates massive neurotransmitter release by two mechanisms: $ Ca^{2+} $‐dependent and ‐independent. Our studies on norepinephrine secretion from nerve terminals now reveal the different molecular basis of these two actions. The $ Ca^{2+} $‐dependent LTX‐evoked vesicle exocytosis (abolished by botulinum neurotoxins) is 10‐fold more sensitive to external $ Ca^{2+} $ than secretion triggered by depolarization or A23187; it does not, however, depend on the cation entry into terminals but requires intracellular $ Ca^{2+} $ and is blocked by drugs depleting $ Ca^{2+} $ stores and by inhibitors of phospholipase C (PLC). These data, together with binding studies, prove that latrophilin, which is linked to G proteins and inositol polyphosphate production, is the major functional LTX receptor. The $ Ca^{2+} $‐independent LTX‐stimulated release is not inhibited by botulinum neurotoxins or drugs interfering with $ Ca^{2+} $ metabolism and occurs via pores in the presynaptic membrane, large enough to allow efflux of neurotransmitters and other small molecules from the cytoplasm. Our results unite previously contradictory data about the toxin's effects and suggest that LTX‐stimulated exocytosis depends upon the co‐operative action of external and intracellular $ Ca^{2+} $ involving G proteins and PLC, whereas the $ Ca^{2+} $‐independent release is largely non‐vesicular. Ca (dpeaa)DE-He213 exocytosis (dpeaa)DE-He213 latrophilin (dpeaa)DE-He213 α‐latrotoxin (dpeaa)DE-He213 norepinephrine release (dpeaa)DE-He213 Meunier, Frédéric A. verfasserin aut Ashton, Anthony C. verfasserin aut Matsushita, Hiroaki verfasserin aut Hirst, Warren D. verfasserin aut Lelianova, Vera G. verfasserin aut Wilkin, Graham P. verfasserin aut Dolly, J.Oliver verfasserin aut Ushkaryov, Yuri A. verfasserin aut Enthalten in The EMBO Journal Nature Publishing Group UK, 2023 17(1998), 14 vom: 15. Juli, Seite 3909-3920 (DE-627)266022529 (DE-600)1467419-1 1460-2075 nnns volume:17 year:1998 number:14 day:15 month:07 pages:3909-3920 https://dx.doi.org/10.1093/emboj/17.14.3909 X:SPRINGER Resolving-System lizenzpflichtig Volltext SYSFLAG_0 GBV_SPRINGER GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_72 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_161 GBV_ILN_168 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_252 GBV_ILN_266 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2018 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4029 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4116 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4155 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4311 GBV_ILN_4313 GBV_ILN_4314 GBV_ILN_4318 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4393 GBV_ILN_4598 GBV_ILN_4700 AR 17 1998 14 15 07 3909-3920 |
| spelling |
10.1093/emboj/17.14.3909 doi (DE-627)SPR058000623 (SPR)17.14.3909-e DE-627 ger DE-627 rakwb eng Davletov, Bazbek A. verfasserin aut Vesicle exocytosis stimulated by α‐latrotoxin is mediated by latrophilin and requires both external and stored $ Ca^{2+} $ 1998 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © European Molecular Biology Organization 1998 Abstract α‐Latrotoxin (LTX) stimulates massive neurotransmitter release by two mechanisms: $ Ca^{2+} $‐dependent and ‐independent. Our studies on norepinephrine secretion from nerve terminals now reveal the different molecular basis of these two actions. The $ Ca^{2+} $‐dependent LTX‐evoked vesicle exocytosis (abolished by botulinum neurotoxins) is 10‐fold more sensitive to external $ Ca^{2+} $ than secretion triggered by depolarization or A23187; it does not, however, depend on the cation entry into terminals but requires intracellular $ Ca^{2+} $ and is blocked by drugs depleting $ Ca^{2+} $ stores and by inhibitors of phospholipase C (PLC). These data, together with binding studies, prove that latrophilin, which is linked to G proteins and inositol polyphosphate production, is the major functional LTX receptor. The $ Ca^{2+} $‐independent LTX‐stimulated release is not inhibited by botulinum neurotoxins or drugs interfering with $ Ca^{2+} $ metabolism and occurs via pores in the presynaptic membrane, large enough to allow efflux of neurotransmitters and other small molecules from the cytoplasm. Our results unite previously contradictory data about the toxin's effects and suggest that LTX‐stimulated exocytosis depends upon the co‐operative action of external and intracellular $ Ca^{2+} $ involving G proteins and PLC, whereas the $ Ca^{2+} $‐independent release is largely non‐vesicular. Ca (dpeaa)DE-He213 exocytosis (dpeaa)DE-He213 latrophilin (dpeaa)DE-He213 α‐latrotoxin (dpeaa)DE-He213 norepinephrine release (dpeaa)DE-He213 Meunier, Frédéric A. verfasserin aut Ashton, Anthony C. verfasserin aut Matsushita, Hiroaki verfasserin aut Hirst, Warren D. verfasserin aut Lelianova, Vera G. verfasserin aut Wilkin, Graham P. verfasserin aut Dolly, J.Oliver verfasserin aut Ushkaryov, Yuri A. verfasserin aut Enthalten in The EMBO Journal Nature Publishing Group UK, 2023 17(1998), 14 vom: 15. Juli, Seite 3909-3920 (DE-627)266022529 (DE-600)1467419-1 1460-2075 nnns volume:17 year:1998 number:14 day:15 month:07 pages:3909-3920 https://dx.doi.org/10.1093/emboj/17.14.3909 X:SPRINGER Resolving-System lizenzpflichtig Volltext SYSFLAG_0 GBV_SPRINGER GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_72 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_161 GBV_ILN_168 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_252 GBV_ILN_266 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2018 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4029 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4116 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4155 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4311 GBV_ILN_4313 GBV_ILN_4314 GBV_ILN_4318 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4393 GBV_ILN_4598 GBV_ILN_4700 AR 17 1998 14 15 07 3909-3920 |
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10.1093/emboj/17.14.3909 doi (DE-627)SPR058000623 (SPR)17.14.3909-e DE-627 ger DE-627 rakwb eng Davletov, Bazbek A. verfasserin aut Vesicle exocytosis stimulated by α‐latrotoxin is mediated by latrophilin and requires both external and stored $ Ca^{2+} $ 1998 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © European Molecular Biology Organization 1998 Abstract α‐Latrotoxin (LTX) stimulates massive neurotransmitter release by two mechanisms: $ Ca^{2+} $‐dependent and ‐independent. Our studies on norepinephrine secretion from nerve terminals now reveal the different molecular basis of these two actions. The $ Ca^{2+} $‐dependent LTX‐evoked vesicle exocytosis (abolished by botulinum neurotoxins) is 10‐fold more sensitive to external $ Ca^{2+} $ than secretion triggered by depolarization or A23187; it does not, however, depend on the cation entry into terminals but requires intracellular $ Ca^{2+} $ and is blocked by drugs depleting $ Ca^{2+} $ stores and by inhibitors of phospholipase C (PLC). These data, together with binding studies, prove that latrophilin, which is linked to G proteins and inositol polyphosphate production, is the major functional LTX receptor. The $ Ca^{2+} $‐independent LTX‐stimulated release is not inhibited by botulinum neurotoxins or drugs interfering with $ Ca^{2+} $ metabolism and occurs via pores in the presynaptic membrane, large enough to allow efflux of neurotransmitters and other small molecules from the cytoplasm. Our results unite previously contradictory data about the toxin's effects and suggest that LTX‐stimulated exocytosis depends upon the co‐operative action of external and intracellular $ Ca^{2+} $ involving G proteins and PLC, whereas the $ Ca^{2+} $‐independent release is largely non‐vesicular. Ca (dpeaa)DE-He213 exocytosis (dpeaa)DE-He213 latrophilin (dpeaa)DE-He213 α‐latrotoxin (dpeaa)DE-He213 norepinephrine release (dpeaa)DE-He213 Meunier, Frédéric A. verfasserin aut Ashton, Anthony C. verfasserin aut Matsushita, Hiroaki verfasserin aut Hirst, Warren D. verfasserin aut Lelianova, Vera G. verfasserin aut Wilkin, Graham P. verfasserin aut Dolly, J.Oliver verfasserin aut Ushkaryov, Yuri A. verfasserin aut Enthalten in The EMBO Journal Nature Publishing Group UK, 2023 17(1998), 14 vom: 15. Juli, Seite 3909-3920 (DE-627)266022529 (DE-600)1467419-1 1460-2075 nnns volume:17 year:1998 number:14 day:15 month:07 pages:3909-3920 https://dx.doi.org/10.1093/emboj/17.14.3909 X:SPRINGER Resolving-System lizenzpflichtig Volltext SYSFLAG_0 GBV_SPRINGER GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_72 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_161 GBV_ILN_168 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_252 GBV_ILN_266 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2018 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4029 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4116 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4155 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4311 GBV_ILN_4313 GBV_ILN_4314 GBV_ILN_4318 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4393 GBV_ILN_4598 GBV_ILN_4700 AR 17 1998 14 15 07 3909-3920 |
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10.1093/emboj/17.14.3909 doi (DE-627)SPR058000623 (SPR)17.14.3909-e DE-627 ger DE-627 rakwb eng Davletov, Bazbek A. verfasserin aut Vesicle exocytosis stimulated by α‐latrotoxin is mediated by latrophilin and requires both external and stored $ Ca^{2+} $ 1998 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © European Molecular Biology Organization 1998 Abstract α‐Latrotoxin (LTX) stimulates massive neurotransmitter release by two mechanisms: $ Ca^{2+} $‐dependent and ‐independent. Our studies on norepinephrine secretion from nerve terminals now reveal the different molecular basis of these two actions. The $ Ca^{2+} $‐dependent LTX‐evoked vesicle exocytosis (abolished by botulinum neurotoxins) is 10‐fold more sensitive to external $ Ca^{2+} $ than secretion triggered by depolarization or A23187; it does not, however, depend on the cation entry into terminals but requires intracellular $ Ca^{2+} $ and is blocked by drugs depleting $ Ca^{2+} $ stores and by inhibitors of phospholipase C (PLC). These data, together with binding studies, prove that latrophilin, which is linked to G proteins and inositol polyphosphate production, is the major functional LTX receptor. The $ Ca^{2+} $‐independent LTX‐stimulated release is not inhibited by botulinum neurotoxins or drugs interfering with $ Ca^{2+} $ metabolism and occurs via pores in the presynaptic membrane, large enough to allow efflux of neurotransmitters and other small molecules from the cytoplasm. Our results unite previously contradictory data about the toxin's effects and suggest that LTX‐stimulated exocytosis depends upon the co‐operative action of external and intracellular $ Ca^{2+} $ involving G proteins and PLC, whereas the $ Ca^{2+} $‐independent release is largely non‐vesicular. Ca (dpeaa)DE-He213 exocytosis (dpeaa)DE-He213 latrophilin (dpeaa)DE-He213 α‐latrotoxin (dpeaa)DE-He213 norepinephrine release (dpeaa)DE-He213 Meunier, Frédéric A. verfasserin aut Ashton, Anthony C. verfasserin aut Matsushita, Hiroaki verfasserin aut Hirst, Warren D. verfasserin aut Lelianova, Vera G. verfasserin aut Wilkin, Graham P. verfasserin aut Dolly, J.Oliver verfasserin aut Ushkaryov, Yuri A. verfasserin aut Enthalten in The EMBO Journal Nature Publishing Group UK, 2023 17(1998), 14 vom: 15. Juli, Seite 3909-3920 (DE-627)266022529 (DE-600)1467419-1 1460-2075 nnns volume:17 year:1998 number:14 day:15 month:07 pages:3909-3920 https://dx.doi.org/10.1093/emboj/17.14.3909 X:SPRINGER Resolving-System lizenzpflichtig Volltext SYSFLAG_0 GBV_SPRINGER GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_72 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_161 GBV_ILN_168 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_252 GBV_ILN_266 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2018 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4029 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4116 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4155 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4311 GBV_ILN_4313 GBV_ILN_4314 GBV_ILN_4318 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4393 GBV_ILN_4598 GBV_ILN_4700 AR 17 1998 14 15 07 3909-3920 |
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10.1093/emboj/17.14.3909 doi (DE-627)SPR058000623 (SPR)17.14.3909-e DE-627 ger DE-627 rakwb eng Davletov, Bazbek A. verfasserin aut Vesicle exocytosis stimulated by α‐latrotoxin is mediated by latrophilin and requires both external and stored $ Ca^{2+} $ 1998 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © European Molecular Biology Organization 1998 Abstract α‐Latrotoxin (LTX) stimulates massive neurotransmitter release by two mechanisms: $ Ca^{2+} $‐dependent and ‐independent. Our studies on norepinephrine secretion from nerve terminals now reveal the different molecular basis of these two actions. The $ Ca^{2+} $‐dependent LTX‐evoked vesicle exocytosis (abolished by botulinum neurotoxins) is 10‐fold more sensitive to external $ Ca^{2+} $ than secretion triggered by depolarization or A23187; it does not, however, depend on the cation entry into terminals but requires intracellular $ Ca^{2+} $ and is blocked by drugs depleting $ Ca^{2+} $ stores and by inhibitors of phospholipase C (PLC). These data, together with binding studies, prove that latrophilin, which is linked to G proteins and inositol polyphosphate production, is the major functional LTX receptor. The $ Ca^{2+} $‐independent LTX‐stimulated release is not inhibited by botulinum neurotoxins or drugs interfering with $ Ca^{2+} $ metabolism and occurs via pores in the presynaptic membrane, large enough to allow efflux of neurotransmitters and other small molecules from the cytoplasm. Our results unite previously contradictory data about the toxin's effects and suggest that LTX‐stimulated exocytosis depends upon the co‐operative action of external and intracellular $ Ca^{2+} $ involving G proteins and PLC, whereas the $ Ca^{2+} $‐independent release is largely non‐vesicular. Ca (dpeaa)DE-He213 exocytosis (dpeaa)DE-He213 latrophilin (dpeaa)DE-He213 α‐latrotoxin (dpeaa)DE-He213 norepinephrine release (dpeaa)DE-He213 Meunier, Frédéric A. verfasserin aut Ashton, Anthony C. verfasserin aut Matsushita, Hiroaki verfasserin aut Hirst, Warren D. verfasserin aut Lelianova, Vera G. verfasserin aut Wilkin, Graham P. verfasserin aut Dolly, J.Oliver verfasserin aut Ushkaryov, Yuri A. verfasserin aut Enthalten in The EMBO Journal Nature Publishing Group UK, 2023 17(1998), 14 vom: 15. Juli, Seite 3909-3920 (DE-627)266022529 (DE-600)1467419-1 1460-2075 nnns volume:17 year:1998 number:14 day:15 month:07 pages:3909-3920 https://dx.doi.org/10.1093/emboj/17.14.3909 X:SPRINGER Resolving-System lizenzpflichtig Volltext SYSFLAG_0 GBV_SPRINGER GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_72 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_161 GBV_ILN_168 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_252 GBV_ILN_266 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2018 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4029 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4116 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4155 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4311 GBV_ILN_4313 GBV_ILN_4314 GBV_ILN_4318 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4393 GBV_ILN_4598 GBV_ILN_4700 AR 17 1998 14 15 07 3909-3920 |
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English |
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Enthalten in The EMBO Journal 17(1998), 14 vom: 15. Juli, Seite 3909-3920 volume:17 year:1998 number:14 day:15 month:07 pages:3909-3920 |
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Enthalten in The EMBO Journal 17(1998), 14 vom: 15. Juli, Seite 3909-3920 volume:17 year:1998 number:14 day:15 month:07 pages:3909-3920 |
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Ca exocytosis latrophilin α‐latrotoxin norepinephrine release |
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The EMBO Journal |
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Davletov, Bazbek A. @@aut@@ Meunier, Frédéric A. @@aut@@ Ashton, Anthony C. @@aut@@ Matsushita, Hiroaki @@aut@@ Hirst, Warren D. @@aut@@ Lelianova, Vera G. @@aut@@ Wilkin, Graham P. @@aut@@ Dolly, J.Oliver @@aut@@ Ushkaryov, Yuri A. @@aut@@ |
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1998-07-15T00:00:00Z |
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Our studies on norepinephrine secretion from nerve terminals now reveal the different molecular basis of these two actions. The $ Ca^{2+} $‐dependent LTX‐evoked vesicle exocytosis (abolished by botulinum neurotoxins) is 10‐fold more sensitive to external $ Ca^{2+} $ than secretion triggered by depolarization or A23187; it does not, however, depend on the cation entry into terminals but requires intracellular $ Ca^{2+} $ and is blocked by drugs depleting $ Ca^{2+} $ stores and by inhibitors of phospholipase C (PLC). These data, together with binding studies, prove that latrophilin, which is linked to G proteins and inositol polyphosphate production, is the major functional LTX receptor. The $ Ca^{2+} $‐independent LTX‐stimulated release is not inhibited by botulinum neurotoxins or drugs interfering with $ Ca^{2+} $ metabolism and occurs via pores in the presynaptic membrane, large enough to allow efflux of neurotransmitters and other small molecules from the cytoplasm. 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Davletov, Bazbek A. |
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Davletov, Bazbek A. misc Ca misc exocytosis misc latrophilin misc α‐latrotoxin misc norepinephrine release Vesicle exocytosis stimulated by α‐latrotoxin is mediated by latrophilin and requires both external and stored $ Ca^{2+} $ |
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Vesicle exocytosis stimulated by α‐latrotoxin is mediated by latrophilin and requires both external and stored $ Ca^{2+} $ Ca (dpeaa)DE-He213 exocytosis (dpeaa)DE-He213 latrophilin (dpeaa)DE-He213 α‐latrotoxin (dpeaa)DE-He213 norepinephrine release (dpeaa)DE-He213 |
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Vesicle exocytosis stimulated by α‐latrotoxin is mediated by latrophilin and requires both external and stored $ Ca^{2+} $ |
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Vesicle exocytosis stimulated by α‐latrotoxin is mediated by latrophilin and requires both external and stored $ Ca^{2+} $ |
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Davletov, Bazbek A. Meunier, Frédéric A. Ashton, Anthony C. Matsushita, Hiroaki Hirst, Warren D. Lelianova, Vera G. Wilkin, Graham P. Dolly, J.Oliver Ushkaryov, Yuri A. |
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vesicle exocytosis stimulated by α‐latrotoxin is mediated by latrophilin and requires both external and stored $ ca^{2+} $ |
| title_auth |
Vesicle exocytosis stimulated by α‐latrotoxin is mediated by latrophilin and requires both external and stored $ Ca^{2+} $ |
| abstract |
Abstract α‐Latrotoxin (LTX) stimulates massive neurotransmitter release by two mechanisms: $ Ca^{2+} $‐dependent and ‐independent. Our studies on norepinephrine secretion from nerve terminals now reveal the different molecular basis of these two actions. The $ Ca^{2+} $‐dependent LTX‐evoked vesicle exocytosis (abolished by botulinum neurotoxins) is 10‐fold more sensitive to external $ Ca^{2+} $ than secretion triggered by depolarization or A23187; it does not, however, depend on the cation entry into terminals but requires intracellular $ Ca^{2+} $ and is blocked by drugs depleting $ Ca^{2+} $ stores and by inhibitors of phospholipase C (PLC). These data, together with binding studies, prove that latrophilin, which is linked to G proteins and inositol polyphosphate production, is the major functional LTX receptor. The $ Ca^{2+} $‐independent LTX‐stimulated release is not inhibited by botulinum neurotoxins or drugs interfering with $ Ca^{2+} $ metabolism and occurs via pores in the presynaptic membrane, large enough to allow efflux of neurotransmitters and other small molecules from the cytoplasm. Our results unite previously contradictory data about the toxin's effects and suggest that LTX‐stimulated exocytosis depends upon the co‐operative action of external and intracellular $ Ca^{2+} $ involving G proteins and PLC, whereas the $ Ca^{2+} $‐independent release is largely non‐vesicular. © European Molecular Biology Organization 1998 |
| abstractGer |
Abstract α‐Latrotoxin (LTX) stimulates massive neurotransmitter release by two mechanisms: $ Ca^{2+} $‐dependent and ‐independent. Our studies on norepinephrine secretion from nerve terminals now reveal the different molecular basis of these two actions. The $ Ca^{2+} $‐dependent LTX‐evoked vesicle exocytosis (abolished by botulinum neurotoxins) is 10‐fold more sensitive to external $ Ca^{2+} $ than secretion triggered by depolarization or A23187; it does not, however, depend on the cation entry into terminals but requires intracellular $ Ca^{2+} $ and is blocked by drugs depleting $ Ca^{2+} $ stores and by inhibitors of phospholipase C (PLC). These data, together with binding studies, prove that latrophilin, which is linked to G proteins and inositol polyphosphate production, is the major functional LTX receptor. The $ Ca^{2+} $‐independent LTX‐stimulated release is not inhibited by botulinum neurotoxins or drugs interfering with $ Ca^{2+} $ metabolism and occurs via pores in the presynaptic membrane, large enough to allow efflux of neurotransmitters and other small molecules from the cytoplasm. Our results unite previously contradictory data about the toxin's effects and suggest that LTX‐stimulated exocytosis depends upon the co‐operative action of external and intracellular $ Ca^{2+} $ involving G proteins and PLC, whereas the $ Ca^{2+} $‐independent release is largely non‐vesicular. © European Molecular Biology Organization 1998 |
| abstract_unstemmed |
Abstract α‐Latrotoxin (LTX) stimulates massive neurotransmitter release by two mechanisms: $ Ca^{2+} $‐dependent and ‐independent. Our studies on norepinephrine secretion from nerve terminals now reveal the different molecular basis of these two actions. The $ Ca^{2+} $‐dependent LTX‐evoked vesicle exocytosis (abolished by botulinum neurotoxins) is 10‐fold more sensitive to external $ Ca^{2+} $ than secretion triggered by depolarization or A23187; it does not, however, depend on the cation entry into terminals but requires intracellular $ Ca^{2+} $ and is blocked by drugs depleting $ Ca^{2+} $ stores and by inhibitors of phospholipase C (PLC). These data, together with binding studies, prove that latrophilin, which is linked to G proteins and inositol polyphosphate production, is the major functional LTX receptor. The $ Ca^{2+} $‐independent LTX‐stimulated release is not inhibited by botulinum neurotoxins or drugs interfering with $ Ca^{2+} $ metabolism and occurs via pores in the presynaptic membrane, large enough to allow efflux of neurotransmitters and other small molecules from the cytoplasm. Our results unite previously contradictory data about the toxin's effects and suggest that LTX‐stimulated exocytosis depends upon the co‐operative action of external and intracellular $ Ca^{2+} $ involving G proteins and PLC, whereas the $ Ca^{2+} $‐independent release is largely non‐vesicular. © European Molecular Biology Organization 1998 |
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Vesicle exocytosis stimulated by α‐latrotoxin is mediated by latrophilin and requires both external and stored $ Ca^{2+} $ |
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Meunier, Frédéric A. Ashton, Anthony C. Matsushita, Hiroaki Hirst, Warren D. Lelianova, Vera G. Wilkin, Graham P. Dolly, J.Oliver Ushkaryov, Yuri A. |
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Meunier, Frédéric A. Ashton, Anthony C. Matsushita, Hiroaki Hirst, Warren D. Lelianova, Vera G. Wilkin, Graham P. Dolly, J.Oliver Ushkaryov, Yuri A. |
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| score |
7.4027834 |