Establishing the transcriptional programme for blood: the SCL stem cell enhancer is regulated by a multiprotein complex containing Ets and GATA factors
Abstract Stem cells are a central feature of metazoan biology. Haematopoietic stem cells (HSCs) represent the best‐characterized example of this phenomenon, but the molecular mechanisms responsible for their formation remain obscure. The stem cell leukaemia (SCL) gene encodes a basic helix–loop–heli...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
Göttgens, Berthold [verfasserIn] Nastos, Aristotelis [verfasserIn] Kinston, Sarah [verfasserIn] Piltz, Sandie [verfasserIn] Delabesse, Eric C.M. [verfasserIn] Stanley, Maureen [verfasserIn] Sanchez, Maria‐Jose [verfasserIn] Ciau‐Uitz, Aldo [verfasserIn] Patient, Roger [verfasserIn] Green, Anthony R. [verfasserIn] |
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| Format: |
E-Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
2002 |
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| Schlagwörter: |
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| Anmerkung: |
© European Molecular Biology Organization 2002 |
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| Übergeordnetes Werk: |
Enthalten in: The EMBO Journal - Nature Publishing Group UK, 2023, 21(2002), 12 vom: 17. Juni, Seite 3039-3050 |
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| Übergeordnetes Werk: |
volume:21 ; year:2002 ; number:12 ; day:17 ; month:06 ; pages:3039-3050 |
| Links: |
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| DOI / URN: |
10.1093/emboj/cdf286 |
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| Katalog-ID: |
SPR058006931 |
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| 100 | 1 | |a Göttgens, Berthold |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Establishing the transcriptional programme for blood: the SCL stem cell enhancer is regulated by a multiprotein complex containing Ets and GATA factors |
| 264 | 1 | |c 2002 | |
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| 500 | |a © European Molecular Biology Organization 2002 | ||
| 520 | |a Abstract Stem cells are a central feature of metazoan biology. Haematopoietic stem cells (HSCs) represent the best‐characterized example of this phenomenon, but the molecular mechanisms responsible for their formation remain obscure. The stem cell leukaemia (SCL) gene encodes a basic helix–loop–helix (bHLH) transcription factor with an essential role in specifying HSCs. Here we have addressed the transcriptional hierarchy responsible for HSC formation by characterizing an SCL 3′ enhancer that targets expression to HSCs and endothelium and their bipotential precursors, the haemangioblast. We have identified three critical motifs, which are essential for enhancer function and bind GATA‐2, Fli‐1 and Elf‐1 in vivo. Our results suggest that these transcription factors are key components of an enhanceosome responsible for activating SCL transcription and establishing the transcriptional programme required for HSC formation. | ||
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| 650 | 4 | |a SCL |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a stem cell |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a transcription |7 (dpeaa)DE-He213 | |
| 700 | 1 | |a Nastos, Aristotelis |e verfasserin |4 aut | |
| 700 | 1 | |a Kinston, Sarah |e verfasserin |4 aut | |
| 700 | 1 | |a Piltz, Sandie |e verfasserin |4 aut | |
| 700 | 1 | |a Delabesse, Eric C.M. |e verfasserin |4 aut | |
| 700 | 1 | |a Stanley, Maureen |e verfasserin |4 aut | |
| 700 | 1 | |a Sanchez, Maria‐Jose |e verfasserin |4 aut | |
| 700 | 1 | |a Ciau‐Uitz, Aldo |e verfasserin |4 aut | |
| 700 | 1 | |a Patient, Roger |e verfasserin |4 aut | |
| 700 | 1 | |a Green, Anthony R. |e verfasserin |4 aut | |
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10.1093/emboj/cdf286 doi (DE-627)SPR058006931 (SPR)cdf286-e DE-627 ger DE-627 rakwb eng Göttgens, Berthold verfasserin aut Establishing the transcriptional programme for blood: the SCL stem cell enhancer is regulated by a multiprotein complex containing Ets and GATA factors 2002 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © European Molecular Biology Organization 2002 Abstract Stem cells are a central feature of metazoan biology. Haematopoietic stem cells (HSCs) represent the best‐characterized example of this phenomenon, but the molecular mechanisms responsible for their formation remain obscure. The stem cell leukaemia (SCL) gene encodes a basic helix–loop–helix (bHLH) transcription factor with an essential role in specifying HSCs. Here we have addressed the transcriptional hierarchy responsible for HSC formation by characterizing an SCL 3′ enhancer that targets expression to HSCs and endothelium and their bipotential precursors, the haemangioblast. We have identified three critical motifs, which are essential for enhancer function and bind GATA‐2, Fli‐1 and Elf‐1 in vivo. Our results suggest that these transcription factors are key components of an enhanceosome responsible for activating SCL transcription and establishing the transcriptional programme required for HSC formation. enhanceosome (dpeaa)DE-He213 SCL (dpeaa)DE-He213 stem cell (dpeaa)DE-He213 transcription (dpeaa)DE-He213 Nastos, Aristotelis verfasserin aut Kinston, Sarah verfasserin aut Piltz, Sandie verfasserin aut Delabesse, Eric C.M. verfasserin aut Stanley, Maureen verfasserin aut Sanchez, Maria‐Jose verfasserin aut Ciau‐Uitz, Aldo verfasserin aut Patient, Roger verfasserin aut Green, Anthony R. verfasserin aut Enthalten in The EMBO Journal Nature Publishing Group UK, 2023 21(2002), 12 vom: 17. Juni, Seite 3039-3050 (DE-627)266022529 (DE-600)1467419-1 1460-2075 nnns volume:21 year:2002 number:12 day:17 month:06 pages:3039-3050 https://dx.doi.org/10.1093/emboj/cdf286 X:SPRINGER Resolving-System lizenzpflichtig Volltext SYSFLAG_0 GBV_SPRINGER GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_72 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_161 GBV_ILN_168 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_252 GBV_ILN_266 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2018 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4029 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4116 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4155 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4311 GBV_ILN_4313 GBV_ILN_4314 GBV_ILN_4318 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4393 GBV_ILN_4598 GBV_ILN_4700 AR 21 2002 12 17 06 3039-3050 |
| spelling |
10.1093/emboj/cdf286 doi (DE-627)SPR058006931 (SPR)cdf286-e DE-627 ger DE-627 rakwb eng Göttgens, Berthold verfasserin aut Establishing the transcriptional programme for blood: the SCL stem cell enhancer is regulated by a multiprotein complex containing Ets and GATA factors 2002 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © European Molecular Biology Organization 2002 Abstract Stem cells are a central feature of metazoan biology. Haematopoietic stem cells (HSCs) represent the best‐characterized example of this phenomenon, but the molecular mechanisms responsible for their formation remain obscure. The stem cell leukaemia (SCL) gene encodes a basic helix–loop–helix (bHLH) transcription factor with an essential role in specifying HSCs. Here we have addressed the transcriptional hierarchy responsible for HSC formation by characterizing an SCL 3′ enhancer that targets expression to HSCs and endothelium and their bipotential precursors, the haemangioblast. We have identified three critical motifs, which are essential for enhancer function and bind GATA‐2, Fli‐1 and Elf‐1 in vivo. Our results suggest that these transcription factors are key components of an enhanceosome responsible for activating SCL transcription and establishing the transcriptional programme required for HSC formation. enhanceosome (dpeaa)DE-He213 SCL (dpeaa)DE-He213 stem cell (dpeaa)DE-He213 transcription (dpeaa)DE-He213 Nastos, Aristotelis verfasserin aut Kinston, Sarah verfasserin aut Piltz, Sandie verfasserin aut Delabesse, Eric C.M. verfasserin aut Stanley, Maureen verfasserin aut Sanchez, Maria‐Jose verfasserin aut Ciau‐Uitz, Aldo verfasserin aut Patient, Roger verfasserin aut Green, Anthony R. verfasserin aut Enthalten in The EMBO Journal Nature Publishing Group UK, 2023 21(2002), 12 vom: 17. Juni, Seite 3039-3050 (DE-627)266022529 (DE-600)1467419-1 1460-2075 nnns volume:21 year:2002 number:12 day:17 month:06 pages:3039-3050 https://dx.doi.org/10.1093/emboj/cdf286 X:SPRINGER Resolving-System lizenzpflichtig Volltext SYSFLAG_0 GBV_SPRINGER GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_72 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_161 GBV_ILN_168 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_252 GBV_ILN_266 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2018 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4029 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4116 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4155 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4311 GBV_ILN_4313 GBV_ILN_4314 GBV_ILN_4318 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4393 GBV_ILN_4598 GBV_ILN_4700 AR 21 2002 12 17 06 3039-3050 |
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10.1093/emboj/cdf286 doi (DE-627)SPR058006931 (SPR)cdf286-e DE-627 ger DE-627 rakwb eng Göttgens, Berthold verfasserin aut Establishing the transcriptional programme for blood: the SCL stem cell enhancer is regulated by a multiprotein complex containing Ets and GATA factors 2002 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © European Molecular Biology Organization 2002 Abstract Stem cells are a central feature of metazoan biology. Haematopoietic stem cells (HSCs) represent the best‐characterized example of this phenomenon, but the molecular mechanisms responsible for their formation remain obscure. The stem cell leukaemia (SCL) gene encodes a basic helix–loop–helix (bHLH) transcription factor with an essential role in specifying HSCs. Here we have addressed the transcriptional hierarchy responsible for HSC formation by characterizing an SCL 3′ enhancer that targets expression to HSCs and endothelium and their bipotential precursors, the haemangioblast. We have identified three critical motifs, which are essential for enhancer function and bind GATA‐2, Fli‐1 and Elf‐1 in vivo. Our results suggest that these transcription factors are key components of an enhanceosome responsible for activating SCL transcription and establishing the transcriptional programme required for HSC formation. enhanceosome (dpeaa)DE-He213 SCL (dpeaa)DE-He213 stem cell (dpeaa)DE-He213 transcription (dpeaa)DE-He213 Nastos, Aristotelis verfasserin aut Kinston, Sarah verfasserin aut Piltz, Sandie verfasserin aut Delabesse, Eric C.M. verfasserin aut Stanley, Maureen verfasserin aut Sanchez, Maria‐Jose verfasserin aut Ciau‐Uitz, Aldo verfasserin aut Patient, Roger verfasserin aut Green, Anthony R. verfasserin aut Enthalten in The EMBO Journal Nature Publishing Group UK, 2023 21(2002), 12 vom: 17. Juni, Seite 3039-3050 (DE-627)266022529 (DE-600)1467419-1 1460-2075 nnns volume:21 year:2002 number:12 day:17 month:06 pages:3039-3050 https://dx.doi.org/10.1093/emboj/cdf286 X:SPRINGER Resolving-System lizenzpflichtig Volltext SYSFLAG_0 GBV_SPRINGER GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_72 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_161 GBV_ILN_168 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_252 GBV_ILN_266 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2018 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4029 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4116 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4155 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4311 GBV_ILN_4313 GBV_ILN_4314 GBV_ILN_4318 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4393 GBV_ILN_4598 GBV_ILN_4700 AR 21 2002 12 17 06 3039-3050 |
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10.1093/emboj/cdf286 doi (DE-627)SPR058006931 (SPR)cdf286-e DE-627 ger DE-627 rakwb eng Göttgens, Berthold verfasserin aut Establishing the transcriptional programme for blood: the SCL stem cell enhancer is regulated by a multiprotein complex containing Ets and GATA factors 2002 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © European Molecular Biology Organization 2002 Abstract Stem cells are a central feature of metazoan biology. Haematopoietic stem cells (HSCs) represent the best‐characterized example of this phenomenon, but the molecular mechanisms responsible for their formation remain obscure. The stem cell leukaemia (SCL) gene encodes a basic helix–loop–helix (bHLH) transcription factor with an essential role in specifying HSCs. Here we have addressed the transcriptional hierarchy responsible for HSC formation by characterizing an SCL 3′ enhancer that targets expression to HSCs and endothelium and their bipotential precursors, the haemangioblast. We have identified three critical motifs, which are essential for enhancer function and bind GATA‐2, Fli‐1 and Elf‐1 in vivo. Our results suggest that these transcription factors are key components of an enhanceosome responsible for activating SCL transcription and establishing the transcriptional programme required for HSC formation. enhanceosome (dpeaa)DE-He213 SCL (dpeaa)DE-He213 stem cell (dpeaa)DE-He213 transcription (dpeaa)DE-He213 Nastos, Aristotelis verfasserin aut Kinston, Sarah verfasserin aut Piltz, Sandie verfasserin aut Delabesse, Eric C.M. verfasserin aut Stanley, Maureen verfasserin aut Sanchez, Maria‐Jose verfasserin aut Ciau‐Uitz, Aldo verfasserin aut Patient, Roger verfasserin aut Green, Anthony R. verfasserin aut Enthalten in The EMBO Journal Nature Publishing Group UK, 2023 21(2002), 12 vom: 17. Juni, Seite 3039-3050 (DE-627)266022529 (DE-600)1467419-1 1460-2075 nnns volume:21 year:2002 number:12 day:17 month:06 pages:3039-3050 https://dx.doi.org/10.1093/emboj/cdf286 X:SPRINGER Resolving-System lizenzpflichtig Volltext SYSFLAG_0 GBV_SPRINGER GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_72 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_161 GBV_ILN_168 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_252 GBV_ILN_266 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2018 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4029 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4116 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4155 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4311 GBV_ILN_4313 GBV_ILN_4314 GBV_ILN_4318 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4393 GBV_ILN_4598 GBV_ILN_4700 AR 21 2002 12 17 06 3039-3050 |
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10.1093/emboj/cdf286 doi (DE-627)SPR058006931 (SPR)cdf286-e DE-627 ger DE-627 rakwb eng Göttgens, Berthold verfasserin aut Establishing the transcriptional programme for blood: the SCL stem cell enhancer is regulated by a multiprotein complex containing Ets and GATA factors 2002 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © European Molecular Biology Organization 2002 Abstract Stem cells are a central feature of metazoan biology. Haematopoietic stem cells (HSCs) represent the best‐characterized example of this phenomenon, but the molecular mechanisms responsible for their formation remain obscure. The stem cell leukaemia (SCL) gene encodes a basic helix–loop–helix (bHLH) transcription factor with an essential role in specifying HSCs. Here we have addressed the transcriptional hierarchy responsible for HSC formation by characterizing an SCL 3′ enhancer that targets expression to HSCs and endothelium and their bipotential precursors, the haemangioblast. We have identified three critical motifs, which are essential for enhancer function and bind GATA‐2, Fli‐1 and Elf‐1 in vivo. Our results suggest that these transcription factors are key components of an enhanceosome responsible for activating SCL transcription and establishing the transcriptional programme required for HSC formation. enhanceosome (dpeaa)DE-He213 SCL (dpeaa)DE-He213 stem cell (dpeaa)DE-He213 transcription (dpeaa)DE-He213 Nastos, Aristotelis verfasserin aut Kinston, Sarah verfasserin aut Piltz, Sandie verfasserin aut Delabesse, Eric C.M. verfasserin aut Stanley, Maureen verfasserin aut Sanchez, Maria‐Jose verfasserin aut Ciau‐Uitz, Aldo verfasserin aut Patient, Roger verfasserin aut Green, Anthony R. verfasserin aut Enthalten in The EMBO Journal Nature Publishing Group UK, 2023 21(2002), 12 vom: 17. Juni, Seite 3039-3050 (DE-627)266022529 (DE-600)1467419-1 1460-2075 nnns volume:21 year:2002 number:12 day:17 month:06 pages:3039-3050 https://dx.doi.org/10.1093/emboj/cdf286 X:SPRINGER Resolving-System lizenzpflichtig Volltext SYSFLAG_0 GBV_SPRINGER GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_72 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_161 GBV_ILN_168 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_252 GBV_ILN_266 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2018 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4029 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4116 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4155 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4311 GBV_ILN_4313 GBV_ILN_4314 GBV_ILN_4318 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4393 GBV_ILN_4598 GBV_ILN_4700 AR 21 2002 12 17 06 3039-3050 |
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Enthalten in The EMBO Journal 21(2002), 12 vom: 17. Juni, Seite 3039-3050 volume:21 year:2002 number:12 day:17 month:06 pages:3039-3050 |
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Göttgens, Berthold @@aut@@ Nastos, Aristotelis @@aut@@ Kinston, Sarah @@aut@@ Piltz, Sandie @@aut@@ Delabesse, Eric C.M. @@aut@@ Stanley, Maureen @@aut@@ Sanchez, Maria‐Jose @@aut@@ Ciau‐Uitz, Aldo @@aut@@ Patient, Roger @@aut@@ Green, Anthony R. @@aut@@ |
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Haematopoietic stem cells (HSCs) represent the best‐characterized example of this phenomenon, but the molecular mechanisms responsible for their formation remain obscure. The stem cell leukaemia (SCL) gene encodes a basic helix–loop–helix (bHLH) transcription factor with an essential role in specifying HSCs. Here we have addressed the transcriptional hierarchy responsible for HSC formation by characterizing an SCL 3′ enhancer that targets expression to HSCs and endothelium and their bipotential precursors, the haemangioblast. We have identified three critical motifs, which are essential for enhancer function and bind GATA‐2, Fli‐1 and Elf‐1 in vivo. 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| author |
Göttgens, Berthold |
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Göttgens, Berthold misc enhanceosome misc SCL misc stem cell misc transcription Establishing the transcriptional programme for blood: the SCL stem cell enhancer is regulated by a multiprotein complex containing Ets and GATA factors |
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Establishing the transcriptional programme for blood: the SCL stem cell enhancer is regulated by a multiprotein complex containing Ets and GATA factors enhanceosome (dpeaa)DE-He213 SCL (dpeaa)DE-He213 stem cell (dpeaa)DE-He213 transcription (dpeaa)DE-He213 |
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Establishing the transcriptional programme for blood: the SCL stem cell enhancer is regulated by a multiprotein complex containing Ets and GATA factors |
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Establishing the transcriptional programme for blood: the SCL stem cell enhancer is regulated by a multiprotein complex containing Ets and GATA factors |
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Göttgens, Berthold |
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Göttgens, Berthold Nastos, Aristotelis Kinston, Sarah Piltz, Sandie Delabesse, Eric C.M. Stanley, Maureen Sanchez, Maria‐Jose Ciau‐Uitz, Aldo Patient, Roger Green, Anthony R. |
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Elektronische Aufsätze |
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Göttgens, Berthold |
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10.1093/emboj/cdf286 |
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verfasserin |
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establishing the transcriptional programme for blood: the scl stem cell enhancer is regulated by a multiprotein complex containing ets and gata factors |
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Establishing the transcriptional programme for blood: the SCL stem cell enhancer is regulated by a multiprotein complex containing Ets and GATA factors |
| abstract |
Abstract Stem cells are a central feature of metazoan biology. Haematopoietic stem cells (HSCs) represent the best‐characterized example of this phenomenon, but the molecular mechanisms responsible for their formation remain obscure. The stem cell leukaemia (SCL) gene encodes a basic helix–loop–helix (bHLH) transcription factor with an essential role in specifying HSCs. Here we have addressed the transcriptional hierarchy responsible for HSC formation by characterizing an SCL 3′ enhancer that targets expression to HSCs and endothelium and their bipotential precursors, the haemangioblast. We have identified three critical motifs, which are essential for enhancer function and bind GATA‐2, Fli‐1 and Elf‐1 in vivo. Our results suggest that these transcription factors are key components of an enhanceosome responsible for activating SCL transcription and establishing the transcriptional programme required for HSC formation. © European Molecular Biology Organization 2002 |
| abstractGer |
Abstract Stem cells are a central feature of metazoan biology. Haematopoietic stem cells (HSCs) represent the best‐characterized example of this phenomenon, but the molecular mechanisms responsible for their formation remain obscure. The stem cell leukaemia (SCL) gene encodes a basic helix–loop–helix (bHLH) transcription factor with an essential role in specifying HSCs. Here we have addressed the transcriptional hierarchy responsible for HSC formation by characterizing an SCL 3′ enhancer that targets expression to HSCs and endothelium and their bipotential precursors, the haemangioblast. We have identified three critical motifs, which are essential for enhancer function and bind GATA‐2, Fli‐1 and Elf‐1 in vivo. Our results suggest that these transcription factors are key components of an enhanceosome responsible for activating SCL transcription and establishing the transcriptional programme required for HSC formation. © European Molecular Biology Organization 2002 |
| abstract_unstemmed |
Abstract Stem cells are a central feature of metazoan biology. Haematopoietic stem cells (HSCs) represent the best‐characterized example of this phenomenon, but the molecular mechanisms responsible for their formation remain obscure. The stem cell leukaemia (SCL) gene encodes a basic helix–loop–helix (bHLH) transcription factor with an essential role in specifying HSCs. Here we have addressed the transcriptional hierarchy responsible for HSC formation by characterizing an SCL 3′ enhancer that targets expression to HSCs and endothelium and their bipotential precursors, the haemangioblast. We have identified three critical motifs, which are essential for enhancer function and bind GATA‐2, Fli‐1 and Elf‐1 in vivo. Our results suggest that these transcription factors are key components of an enhanceosome responsible for activating SCL transcription and establishing the transcriptional programme required for HSC formation. © European Molecular Biology Organization 2002 |
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| title_short |
Establishing the transcriptional programme for blood: the SCL stem cell enhancer is regulated by a multiprotein complex containing Ets and GATA factors |
| url |
https://dx.doi.org/10.1093/emboj/cdf286 |
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| author2 |
Nastos, Aristotelis Kinston, Sarah Piltz, Sandie Delabesse, Eric C.M. Stanley, Maureen Sanchez, Maria‐Jose Ciau‐Uitz, Aldo Patient, Roger Green, Anthony R. |
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Nastos, Aristotelis Kinston, Sarah Piltz, Sandie Delabesse, Eric C.M. Stanley, Maureen Sanchez, Maria‐Jose Ciau‐Uitz, Aldo Patient, Roger Green, Anthony R. |
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10.1093/emboj/cdf286 |
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| score |
7.3998413 |