Structural basis for recruitment of glycogen synthase kinase 3β to the axin—APC scaffold complex
Abstract Glycogen synthase kinase 3β (GSK3β) is a serine/threonine kinase involved in insulin, growth factor and Wnt signalling. In Wnt signalling, GSK3β is recruited to a multiprotein complex via interaction with axin, where it hyperphosphorylates β‐catenin, marking it for ubiquitylation and destru...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
Dajani, Rana [verfasserIn] Fraser, Elizabeth [verfasserIn] Roe, S.Mark [verfasserIn] Yeo, Maggie [verfasserIn] Good, Valerie M. [verfasserIn] Thompson, Vivienne [verfasserIn] Dale, Trevor C. [verfasserIn] Pearl, Laurence H. [verfasserIn] |
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| Format: |
E-Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
2003 |
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| Schlagwörter: |
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| Anmerkung: |
© European Molecular Biology Organization 2003 |
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| Übergeordnetes Werk: |
Enthalten in: The EMBO Journal - Nature Publishing Group UK, 2023, 22(2003), 3 vom: 03. Feb., Seite 494-501 |
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| Übergeordnetes Werk: |
volume:22 ; year:2003 ; number:3 ; day:03 ; month:02 ; pages:494-501 |
| Links: |
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| DOI / URN: |
10.1093/emboj/cdg068 |
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| Katalog-ID: |
SPR058010726 |
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| 100 | 1 | |a Dajani, Rana |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Structural basis for recruitment of glycogen synthase kinase 3β to the axin—APC scaffold complex |
| 264 | 1 | |c 2003 | |
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| 500 | |a © European Molecular Biology Organization 2003 | ||
| 520 | |a Abstract Glycogen synthase kinase 3β (GSK3β) is a serine/threonine kinase involved in insulin, growth factor and Wnt signalling. In Wnt signalling, GSK3β is recruited to a multiprotein complex via interaction with axin, where it hyperphosphorylates β‐catenin, marking it for ubiquitylation and destruction. We have now determined the crystal structure of GSK3β in complex with a minimal GSK3β‐binding segment of axin, at 2.4 Å resolution. The structure confirms the co‐localization of the binding sites for axin and FRAT in the C‐terminal domain of GSK3β, but reveals significant differences in the interactions made by axin and FRAT, mediated by conformational plasticity of the 285—299 loop in GSK3β. Detailed comparison of the axin and FRAT GSK3β complexes allows the generation of highly specific mutations, which abrogate binding of one or the other. Quantitative analysis suggests that the interaction of GSK3β with the axin scaffold enhances phosphorylation of β‐catenin by >20 000‐fold. | ||
| 650 | 4 | |a β‐catenin |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a insulin signalling |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a phosphorylation |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a signal transduction |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a Wnt signalling |7 (dpeaa)DE-He213 | |
| 700 | 1 | |a Fraser, Elizabeth |e verfasserin |4 aut | |
| 700 | 1 | |a Roe, S.Mark |e verfasserin |4 aut | |
| 700 | 1 | |a Yeo, Maggie |e verfasserin |4 aut | |
| 700 | 1 | |a Good, Valerie M. |e verfasserin |4 aut | |
| 700 | 1 | |a Thompson, Vivienne |e verfasserin |4 aut | |
| 700 | 1 | |a Dale, Trevor C. |e verfasserin |4 aut | |
| 700 | 1 | |a Pearl, Laurence H. |e verfasserin |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t The EMBO Journal |d Nature Publishing Group UK, 2023 |g 22(2003), 3 vom: 03. Feb., Seite 494-501 |w (DE-627)266022529 |w (DE-600)1467419-1 |x 1460-2075 |7 nnns |
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10.1093/emboj/cdg068 doi (DE-627)SPR058010726 (SPR)cdg068-e DE-627 ger DE-627 rakwb eng Dajani, Rana verfasserin aut Structural basis for recruitment of glycogen synthase kinase 3β to the axin—APC scaffold complex 2003 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © European Molecular Biology Organization 2003 Abstract Glycogen synthase kinase 3β (GSK3β) is a serine/threonine kinase involved in insulin, growth factor and Wnt signalling. In Wnt signalling, GSK3β is recruited to a multiprotein complex via interaction with axin, where it hyperphosphorylates β‐catenin, marking it for ubiquitylation and destruction. We have now determined the crystal structure of GSK3β in complex with a minimal GSK3β‐binding segment of axin, at 2.4 Å resolution. The structure confirms the co‐localization of the binding sites for axin and FRAT in the C‐terminal domain of GSK3β, but reveals significant differences in the interactions made by axin and FRAT, mediated by conformational plasticity of the 285—299 loop in GSK3β. Detailed comparison of the axin and FRAT GSK3β complexes allows the generation of highly specific mutations, which abrogate binding of one or the other. Quantitative analysis suggests that the interaction of GSK3β with the axin scaffold enhances phosphorylation of β‐catenin by >20 000‐fold. β‐catenin (dpeaa)DE-He213 insulin signalling (dpeaa)DE-He213 phosphorylation (dpeaa)DE-He213 signal transduction (dpeaa)DE-He213 Wnt signalling (dpeaa)DE-He213 Fraser, Elizabeth verfasserin aut Roe, S.Mark verfasserin aut Yeo, Maggie verfasserin aut Good, Valerie M. verfasserin aut Thompson, Vivienne verfasserin aut Dale, Trevor C. verfasserin aut Pearl, Laurence H. verfasserin aut Enthalten in The EMBO Journal Nature Publishing Group UK, 2023 22(2003), 3 vom: 03. Feb., Seite 494-501 (DE-627)266022529 (DE-600)1467419-1 1460-2075 nnns volume:22 year:2003 number:3 day:03 month:02 pages:494-501 https://dx.doi.org/10.1093/emboj/cdg068 X:SPRINGER Resolving-System lizenzpflichtig Volltext SYSFLAG_0 GBV_SPRINGER GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_72 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_161 GBV_ILN_168 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_252 GBV_ILN_266 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2018 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4029 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4116 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4155 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4311 GBV_ILN_4313 GBV_ILN_4314 GBV_ILN_4318 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4393 GBV_ILN_4598 GBV_ILN_4700 AR 22 2003 3 03 02 494-501 |
| spelling |
10.1093/emboj/cdg068 doi (DE-627)SPR058010726 (SPR)cdg068-e DE-627 ger DE-627 rakwb eng Dajani, Rana verfasserin aut Structural basis for recruitment of glycogen synthase kinase 3β to the axin—APC scaffold complex 2003 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © European Molecular Biology Organization 2003 Abstract Glycogen synthase kinase 3β (GSK3β) is a serine/threonine kinase involved in insulin, growth factor and Wnt signalling. In Wnt signalling, GSK3β is recruited to a multiprotein complex via interaction with axin, where it hyperphosphorylates β‐catenin, marking it for ubiquitylation and destruction. We have now determined the crystal structure of GSK3β in complex with a minimal GSK3β‐binding segment of axin, at 2.4 Å resolution. The structure confirms the co‐localization of the binding sites for axin and FRAT in the C‐terminal domain of GSK3β, but reveals significant differences in the interactions made by axin and FRAT, mediated by conformational plasticity of the 285—299 loop in GSK3β. Detailed comparison of the axin and FRAT GSK3β complexes allows the generation of highly specific mutations, which abrogate binding of one or the other. Quantitative analysis suggests that the interaction of GSK3β with the axin scaffold enhances phosphorylation of β‐catenin by >20 000‐fold. β‐catenin (dpeaa)DE-He213 insulin signalling (dpeaa)DE-He213 phosphorylation (dpeaa)DE-He213 signal transduction (dpeaa)DE-He213 Wnt signalling (dpeaa)DE-He213 Fraser, Elizabeth verfasserin aut Roe, S.Mark verfasserin aut Yeo, Maggie verfasserin aut Good, Valerie M. verfasserin aut Thompson, Vivienne verfasserin aut Dale, Trevor C. verfasserin aut Pearl, Laurence H. verfasserin aut Enthalten in The EMBO Journal Nature Publishing Group UK, 2023 22(2003), 3 vom: 03. Feb., Seite 494-501 (DE-627)266022529 (DE-600)1467419-1 1460-2075 nnns volume:22 year:2003 number:3 day:03 month:02 pages:494-501 https://dx.doi.org/10.1093/emboj/cdg068 X:SPRINGER Resolving-System lizenzpflichtig Volltext SYSFLAG_0 GBV_SPRINGER GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_72 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_161 GBV_ILN_168 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_252 GBV_ILN_266 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2018 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4029 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4116 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4155 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4311 GBV_ILN_4313 GBV_ILN_4314 GBV_ILN_4318 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4393 GBV_ILN_4598 GBV_ILN_4700 AR 22 2003 3 03 02 494-501 |
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10.1093/emboj/cdg068 doi (DE-627)SPR058010726 (SPR)cdg068-e DE-627 ger DE-627 rakwb eng Dajani, Rana verfasserin aut Structural basis for recruitment of glycogen synthase kinase 3β to the axin—APC scaffold complex 2003 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © European Molecular Biology Organization 2003 Abstract Glycogen synthase kinase 3β (GSK3β) is a serine/threonine kinase involved in insulin, growth factor and Wnt signalling. In Wnt signalling, GSK3β is recruited to a multiprotein complex via interaction with axin, where it hyperphosphorylates β‐catenin, marking it for ubiquitylation and destruction. We have now determined the crystal structure of GSK3β in complex with a minimal GSK3β‐binding segment of axin, at 2.4 Å resolution. The structure confirms the co‐localization of the binding sites for axin and FRAT in the C‐terminal domain of GSK3β, but reveals significant differences in the interactions made by axin and FRAT, mediated by conformational plasticity of the 285—299 loop in GSK3β. Detailed comparison of the axin and FRAT GSK3β complexes allows the generation of highly specific mutations, which abrogate binding of one or the other. Quantitative analysis suggests that the interaction of GSK3β with the axin scaffold enhances phosphorylation of β‐catenin by >20 000‐fold. β‐catenin (dpeaa)DE-He213 insulin signalling (dpeaa)DE-He213 phosphorylation (dpeaa)DE-He213 signal transduction (dpeaa)DE-He213 Wnt signalling (dpeaa)DE-He213 Fraser, Elizabeth verfasserin aut Roe, S.Mark verfasserin aut Yeo, Maggie verfasserin aut Good, Valerie M. verfasserin aut Thompson, Vivienne verfasserin aut Dale, Trevor C. verfasserin aut Pearl, Laurence H. verfasserin aut Enthalten in The EMBO Journal Nature Publishing Group UK, 2023 22(2003), 3 vom: 03. Feb., Seite 494-501 (DE-627)266022529 (DE-600)1467419-1 1460-2075 nnns volume:22 year:2003 number:3 day:03 month:02 pages:494-501 https://dx.doi.org/10.1093/emboj/cdg068 X:SPRINGER Resolving-System lizenzpflichtig Volltext SYSFLAG_0 GBV_SPRINGER GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_72 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_161 GBV_ILN_168 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_252 GBV_ILN_266 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2018 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4029 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4116 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4155 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4311 GBV_ILN_4313 GBV_ILN_4314 GBV_ILN_4318 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4393 GBV_ILN_4598 GBV_ILN_4700 AR 22 2003 3 03 02 494-501 |
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10.1093/emboj/cdg068 doi (DE-627)SPR058010726 (SPR)cdg068-e DE-627 ger DE-627 rakwb eng Dajani, Rana verfasserin aut Structural basis for recruitment of glycogen synthase kinase 3β to the axin—APC scaffold complex 2003 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © European Molecular Biology Organization 2003 Abstract Glycogen synthase kinase 3β (GSK3β) is a serine/threonine kinase involved in insulin, growth factor and Wnt signalling. In Wnt signalling, GSK3β is recruited to a multiprotein complex via interaction with axin, where it hyperphosphorylates β‐catenin, marking it for ubiquitylation and destruction. We have now determined the crystal structure of GSK3β in complex with a minimal GSK3β‐binding segment of axin, at 2.4 Å resolution. The structure confirms the co‐localization of the binding sites for axin and FRAT in the C‐terminal domain of GSK3β, but reveals significant differences in the interactions made by axin and FRAT, mediated by conformational plasticity of the 285—299 loop in GSK3β. Detailed comparison of the axin and FRAT GSK3β complexes allows the generation of highly specific mutations, which abrogate binding of one or the other. Quantitative analysis suggests that the interaction of GSK3β with the axin scaffold enhances phosphorylation of β‐catenin by >20 000‐fold. β‐catenin (dpeaa)DE-He213 insulin signalling (dpeaa)DE-He213 phosphorylation (dpeaa)DE-He213 signal transduction (dpeaa)DE-He213 Wnt signalling (dpeaa)DE-He213 Fraser, Elizabeth verfasserin aut Roe, S.Mark verfasserin aut Yeo, Maggie verfasserin aut Good, Valerie M. verfasserin aut Thompson, Vivienne verfasserin aut Dale, Trevor C. verfasserin aut Pearl, Laurence H. verfasserin aut Enthalten in The EMBO Journal Nature Publishing Group UK, 2023 22(2003), 3 vom: 03. Feb., Seite 494-501 (DE-627)266022529 (DE-600)1467419-1 1460-2075 nnns volume:22 year:2003 number:3 day:03 month:02 pages:494-501 https://dx.doi.org/10.1093/emboj/cdg068 X:SPRINGER Resolving-System lizenzpflichtig Volltext SYSFLAG_0 GBV_SPRINGER GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_72 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_161 GBV_ILN_168 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_252 GBV_ILN_266 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2018 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4029 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4116 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4155 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4311 GBV_ILN_4313 GBV_ILN_4314 GBV_ILN_4318 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4393 GBV_ILN_4598 GBV_ILN_4700 AR 22 2003 3 03 02 494-501 |
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10.1093/emboj/cdg068 doi (DE-627)SPR058010726 (SPR)cdg068-e DE-627 ger DE-627 rakwb eng Dajani, Rana verfasserin aut Structural basis for recruitment of glycogen synthase kinase 3β to the axin—APC scaffold complex 2003 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © European Molecular Biology Organization 2003 Abstract Glycogen synthase kinase 3β (GSK3β) is a serine/threonine kinase involved in insulin, growth factor and Wnt signalling. In Wnt signalling, GSK3β is recruited to a multiprotein complex via interaction with axin, where it hyperphosphorylates β‐catenin, marking it for ubiquitylation and destruction. We have now determined the crystal structure of GSK3β in complex with a minimal GSK3β‐binding segment of axin, at 2.4 Å resolution. The structure confirms the co‐localization of the binding sites for axin and FRAT in the C‐terminal domain of GSK3β, but reveals significant differences in the interactions made by axin and FRAT, mediated by conformational plasticity of the 285—299 loop in GSK3β. Detailed comparison of the axin and FRAT GSK3β complexes allows the generation of highly specific mutations, which abrogate binding of one or the other. Quantitative analysis suggests that the interaction of GSK3β with the axin scaffold enhances phosphorylation of β‐catenin by >20 000‐fold. β‐catenin (dpeaa)DE-He213 insulin signalling (dpeaa)DE-He213 phosphorylation (dpeaa)DE-He213 signal transduction (dpeaa)DE-He213 Wnt signalling (dpeaa)DE-He213 Fraser, Elizabeth verfasserin aut Roe, S.Mark verfasserin aut Yeo, Maggie verfasserin aut Good, Valerie M. verfasserin aut Thompson, Vivienne verfasserin aut Dale, Trevor C. verfasserin aut Pearl, Laurence H. verfasserin aut Enthalten in The EMBO Journal Nature Publishing Group UK, 2023 22(2003), 3 vom: 03. Feb., Seite 494-501 (DE-627)266022529 (DE-600)1467419-1 1460-2075 nnns volume:22 year:2003 number:3 day:03 month:02 pages:494-501 https://dx.doi.org/10.1093/emboj/cdg068 X:SPRINGER Resolving-System lizenzpflichtig Volltext SYSFLAG_0 GBV_SPRINGER GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_72 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_161 GBV_ILN_168 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_252 GBV_ILN_266 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2018 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4029 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4116 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4155 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4311 GBV_ILN_4313 GBV_ILN_4314 GBV_ILN_4318 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4393 GBV_ILN_4598 GBV_ILN_4700 AR 22 2003 3 03 02 494-501 |
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Dajani, Rana @@aut@@ Fraser, Elizabeth @@aut@@ Roe, S.Mark @@aut@@ Yeo, Maggie @@aut@@ Good, Valerie M. @@aut@@ Thompson, Vivienne @@aut@@ Dale, Trevor C. @@aut@@ Pearl, Laurence H. @@aut@@ |
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Dajani, Rana |
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Dajani, Rana misc β‐catenin misc insulin signalling misc phosphorylation misc signal transduction misc Wnt signalling Structural basis for recruitment of glycogen synthase kinase 3β to the axin—APC scaffold complex |
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Structural basis for recruitment of glycogen synthase kinase 3β to the axin—APC scaffold complex β‐catenin (dpeaa)DE-He213 insulin signalling (dpeaa)DE-He213 phosphorylation (dpeaa)DE-He213 signal transduction (dpeaa)DE-He213 Wnt signalling (dpeaa)DE-He213 |
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Structural basis for recruitment of glycogen synthase kinase 3β to the axin—APC scaffold complex |
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Structural basis for recruitment of glycogen synthase kinase 3β to the axin—APC scaffold complex |
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Dajani, Rana Fraser, Elizabeth Roe, S.Mark Yeo, Maggie Good, Valerie M. Thompson, Vivienne Dale, Trevor C. Pearl, Laurence H. |
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structural basis for recruitment of glycogen synthase kinase 3β to the axin—apc scaffold complex |
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Structural basis for recruitment of glycogen synthase kinase 3β to the axin—APC scaffold complex |
| abstract |
Abstract Glycogen synthase kinase 3β (GSK3β) is a serine/threonine kinase involved in insulin, growth factor and Wnt signalling. In Wnt signalling, GSK3β is recruited to a multiprotein complex via interaction with axin, where it hyperphosphorylates β‐catenin, marking it for ubiquitylation and destruction. We have now determined the crystal structure of GSK3β in complex with a minimal GSK3β‐binding segment of axin, at 2.4 Å resolution. The structure confirms the co‐localization of the binding sites for axin and FRAT in the C‐terminal domain of GSK3β, but reveals significant differences in the interactions made by axin and FRAT, mediated by conformational plasticity of the 285—299 loop in GSK3β. Detailed comparison of the axin and FRAT GSK3β complexes allows the generation of highly specific mutations, which abrogate binding of one or the other. Quantitative analysis suggests that the interaction of GSK3β with the axin scaffold enhances phosphorylation of β‐catenin by >20 000‐fold. © European Molecular Biology Organization 2003 |
| abstractGer |
Abstract Glycogen synthase kinase 3β (GSK3β) is a serine/threonine kinase involved in insulin, growth factor and Wnt signalling. In Wnt signalling, GSK3β is recruited to a multiprotein complex via interaction with axin, where it hyperphosphorylates β‐catenin, marking it for ubiquitylation and destruction. We have now determined the crystal structure of GSK3β in complex with a minimal GSK3β‐binding segment of axin, at 2.4 Å resolution. The structure confirms the co‐localization of the binding sites for axin and FRAT in the C‐terminal domain of GSK3β, but reveals significant differences in the interactions made by axin and FRAT, mediated by conformational plasticity of the 285—299 loop in GSK3β. Detailed comparison of the axin and FRAT GSK3β complexes allows the generation of highly specific mutations, which abrogate binding of one or the other. Quantitative analysis suggests that the interaction of GSK3β with the axin scaffold enhances phosphorylation of β‐catenin by >20 000‐fold. © European Molecular Biology Organization 2003 |
| abstract_unstemmed |
Abstract Glycogen synthase kinase 3β (GSK3β) is a serine/threonine kinase involved in insulin, growth factor and Wnt signalling. In Wnt signalling, GSK3β is recruited to a multiprotein complex via interaction with axin, where it hyperphosphorylates β‐catenin, marking it for ubiquitylation and destruction. We have now determined the crystal structure of GSK3β in complex with a minimal GSK3β‐binding segment of axin, at 2.4 Å resolution. The structure confirms the co‐localization of the binding sites for axin and FRAT in the C‐terminal domain of GSK3β, but reveals significant differences in the interactions made by axin and FRAT, mediated by conformational plasticity of the 285—299 loop in GSK3β. Detailed comparison of the axin and FRAT GSK3β complexes allows the generation of highly specific mutations, which abrogate binding of one or the other. Quantitative analysis suggests that the interaction of GSK3β with the axin scaffold enhances phosphorylation of β‐catenin by >20 000‐fold. © European Molecular Biology Organization 2003 |
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Structural basis for recruitment of glycogen synthase kinase 3β to the axin—APC scaffold complex |
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https://dx.doi.org/10.1093/emboj/cdg068 |
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Fraser, Elizabeth Roe, S.Mark Yeo, Maggie Good, Valerie M. Thompson, Vivienne Dale, Trevor C. Pearl, Laurence H. |
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| score |
7.399928 |