Translation of small downstream ORFs enhances translation of canonical main open reading frames
Abstract In addition to canonical open reading frames (ORFs), thousands of translated small ORFs (containing less than 100 codons) have been identified in untranslated mRNA regions (UTRs) across eukaryotes. Small ORFs in 5′ UTRs (upstream (u)ORFs) often repress translation of the canonical ORF withi...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
Wu, Qiushuang [verfasserIn] Wright, Matthew [verfasserIn] Gogol, Madelaine M [verfasserIn] Bradford, William D [verfasserIn] Zhang, Ning [verfasserIn] Bazzini, Ariel A [verfasserIn] |
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| Format: |
E-Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
2020 |
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| Schlagwörter: |
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| Anmerkung: |
© The Author(s) 2020 |
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| Übergeordnetes Werk: |
Enthalten in: The EMBO Journal - Nature Publishing Group UK, 2023, 39(2020), 17 vom: 03. Aug. |
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| Übergeordnetes Werk: |
volume:39 ; year:2020 ; number:17 ; day:03 ; month:08 |
| Links: |
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| DOI / URN: |
10.15252/embj.2020104763 |
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| Katalog-ID: |
SPR058018522 |
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| 100 | 1 | |a Wu, Qiushuang |e verfasserin |0 (orcid)0000-0002-9301-3630 |4 aut | |
| 245 | 1 | 0 | |a Translation of small downstream ORFs enhances translation of canonical main open reading frames |
| 264 | 1 | |c 2020 | |
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| 337 | |a Computermedien |b c |2 rdamedia | ||
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| 500 | |a © The Author(s) 2020 | ||
| 520 | |a Abstract In addition to canonical open reading frames (ORFs), thousands of translated small ORFs (containing less than 100 codons) have been identified in untranslated mRNA regions (UTRs) across eukaryotes. Small ORFs in 5′ UTRs (upstream (u)ORFs) often repress translation of the canonical ORF within the same mRNA. However, the function of translated small ORFs in the 3′ UTRs (downstream (d)ORFs) is unknown. Contrary to uORFs, we find that translation of dORFs enhances translation of their corresponding canonical ORFs. This translation stimulatory effect of dORFs depends on the number of dORFs, but not the length or peptide they encode. We propose that dORFs represent a new, strong, and universal translation regulatory mechanism in vertebrates. | ||
| 520 | |a Synopsis Translation of small downstream open reading frames (dORFs) in the 3′ untranslated region (UTR) enhances translation of the canonical ORF on the same mRNA, independently of the dORF‐encoded peptide. This conserved and prevalent vertebrate post‐transcriptional regulatory mechanisms contrasts the translation‐repressing effects associated with upstream ORFs. Thousands of small dORFs are translated in human and zebrafish 3′ UTRs.dORF presence, but not the amino acid sequence, is conserved between human and zebrafish.Translation of dORFs enhances translation of the respective canonical ORFs. | ||
| 520 | |a Graphical Abstract In contrast to upstream ORFs, translation of small open reading frames located in 3′ untranslated regions of mRNA promotes translation of the canonical ORF, independent of length or sequence of the encoded peptide. | ||
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| 650 | 4 | |a ribosome profiling |7 (dpeaa)DE-He213 | |
| 650 | 4 | |a translation efficiency |7 (dpeaa)DE-He213 | |
| 700 | 1 | |a Wright, Matthew |e verfasserin |0 (orcid)0000-0002-6634-934X |4 aut | |
| 700 | 1 | |a Gogol, Madelaine M |e verfasserin |4 aut | |
| 700 | 1 | |a Bradford, William D |e verfasserin |4 aut | |
| 700 | 1 | |a Zhang, Ning |e verfasserin |4 aut | |
| 700 | 1 | |a Bazzini, Ariel A |e verfasserin |0 (orcid)0000-0002-2251-5174 |4 aut | |
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10.15252/embj.2020104763 doi (DE-627)SPR058018522 (SPR)embj.2020104763-e DE-627 ger DE-627 rakwb eng Wu, Qiushuang verfasserin (orcid)0000-0002-9301-3630 aut Translation of small downstream ORFs enhances translation of canonical main open reading frames 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s) 2020 Abstract In addition to canonical open reading frames (ORFs), thousands of translated small ORFs (containing less than 100 codons) have been identified in untranslated mRNA regions (UTRs) across eukaryotes. Small ORFs in 5′ UTRs (upstream (u)ORFs) often repress translation of the canonical ORF within the same mRNA. However, the function of translated small ORFs in the 3′ UTRs (downstream (d)ORFs) is unknown. Contrary to uORFs, we find that translation of dORFs enhances translation of their corresponding canonical ORFs. This translation stimulatory effect of dORFs depends on the number of dORFs, but not the length or peptide they encode. We propose that dORFs represent a new, strong, and universal translation regulatory mechanism in vertebrates. Synopsis Translation of small downstream open reading frames (dORFs) in the 3′ untranslated region (UTR) enhances translation of the canonical ORF on the same mRNA, independently of the dORF‐encoded peptide. This conserved and prevalent vertebrate post‐transcriptional regulatory mechanisms contrasts the translation‐repressing effects associated with upstream ORFs. Thousands of small dORFs are translated in human and zebrafish 3′ UTRs.dORF presence, but not the amino acid sequence, is conserved between human and zebrafish.Translation of dORFs enhances translation of the respective canonical ORFs. Graphical Abstract In contrast to upstream ORFs, translation of small open reading frames located in 3′ untranslated regions of mRNA promotes translation of the canonical ORF, independent of length or sequence of the encoded peptide. dORF (dpeaa)DE-He213 ribosome profiling (dpeaa)DE-He213 translation efficiency (dpeaa)DE-He213 Wright, Matthew verfasserin (orcid)0000-0002-6634-934X aut Gogol, Madelaine M verfasserin aut Bradford, William D verfasserin aut Zhang, Ning verfasserin aut Bazzini, Ariel A verfasserin (orcid)0000-0002-2251-5174 aut Enthalten in The EMBO Journal Nature Publishing Group UK, 2023 39(2020), 17 vom: 03. Aug. (DE-627)266022529 (DE-600)1467419-1 1460-2075 nnns volume:39 year:2020 number:17 day:03 month:08 https://dx.doi.org/10.15252/embj.2020104763 X:SPRINGER Resolving-System kostenfrei Volltext SYSFLAG_0 GBV_SPRINGER GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_72 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_161 GBV_ILN_168 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_211 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_252 GBV_ILN_266 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4029 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4116 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4155 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4311 GBV_ILN_4313 GBV_ILN_4314 GBV_ILN_4318 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4393 GBV_ILN_4598 GBV_ILN_4700 AR 39 2020 17 03 08 |
| spelling |
10.15252/embj.2020104763 doi (DE-627)SPR058018522 (SPR)embj.2020104763-e DE-627 ger DE-627 rakwb eng Wu, Qiushuang verfasserin (orcid)0000-0002-9301-3630 aut Translation of small downstream ORFs enhances translation of canonical main open reading frames 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s) 2020 Abstract In addition to canonical open reading frames (ORFs), thousands of translated small ORFs (containing less than 100 codons) have been identified in untranslated mRNA regions (UTRs) across eukaryotes. Small ORFs in 5′ UTRs (upstream (u)ORFs) often repress translation of the canonical ORF within the same mRNA. However, the function of translated small ORFs in the 3′ UTRs (downstream (d)ORFs) is unknown. Contrary to uORFs, we find that translation of dORFs enhances translation of their corresponding canonical ORFs. This translation stimulatory effect of dORFs depends on the number of dORFs, but not the length or peptide they encode. We propose that dORFs represent a new, strong, and universal translation regulatory mechanism in vertebrates. Synopsis Translation of small downstream open reading frames (dORFs) in the 3′ untranslated region (UTR) enhances translation of the canonical ORF on the same mRNA, independently of the dORF‐encoded peptide. This conserved and prevalent vertebrate post‐transcriptional regulatory mechanisms contrasts the translation‐repressing effects associated with upstream ORFs. Thousands of small dORFs are translated in human and zebrafish 3′ UTRs.dORF presence, but not the amino acid sequence, is conserved between human and zebrafish.Translation of dORFs enhances translation of the respective canonical ORFs. Graphical Abstract In contrast to upstream ORFs, translation of small open reading frames located in 3′ untranslated regions of mRNA promotes translation of the canonical ORF, independent of length or sequence of the encoded peptide. dORF (dpeaa)DE-He213 ribosome profiling (dpeaa)DE-He213 translation efficiency (dpeaa)DE-He213 Wright, Matthew verfasserin (orcid)0000-0002-6634-934X aut Gogol, Madelaine M verfasserin aut Bradford, William D verfasserin aut Zhang, Ning verfasserin aut Bazzini, Ariel A verfasserin (orcid)0000-0002-2251-5174 aut Enthalten in The EMBO Journal Nature Publishing Group UK, 2023 39(2020), 17 vom: 03. Aug. (DE-627)266022529 (DE-600)1467419-1 1460-2075 nnns volume:39 year:2020 number:17 day:03 month:08 https://dx.doi.org/10.15252/embj.2020104763 X:SPRINGER Resolving-System kostenfrei Volltext SYSFLAG_0 GBV_SPRINGER GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_72 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_161 GBV_ILN_168 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_211 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_252 GBV_ILN_266 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4029 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4116 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4155 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4311 GBV_ILN_4313 GBV_ILN_4314 GBV_ILN_4318 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4393 GBV_ILN_4598 GBV_ILN_4700 AR 39 2020 17 03 08 |
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10.15252/embj.2020104763 doi (DE-627)SPR058018522 (SPR)embj.2020104763-e DE-627 ger DE-627 rakwb eng Wu, Qiushuang verfasserin (orcid)0000-0002-9301-3630 aut Translation of small downstream ORFs enhances translation of canonical main open reading frames 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s) 2020 Abstract In addition to canonical open reading frames (ORFs), thousands of translated small ORFs (containing less than 100 codons) have been identified in untranslated mRNA regions (UTRs) across eukaryotes. Small ORFs in 5′ UTRs (upstream (u)ORFs) often repress translation of the canonical ORF within the same mRNA. However, the function of translated small ORFs in the 3′ UTRs (downstream (d)ORFs) is unknown. Contrary to uORFs, we find that translation of dORFs enhances translation of their corresponding canonical ORFs. This translation stimulatory effect of dORFs depends on the number of dORFs, but not the length or peptide they encode. We propose that dORFs represent a new, strong, and universal translation regulatory mechanism in vertebrates. Synopsis Translation of small downstream open reading frames (dORFs) in the 3′ untranslated region (UTR) enhances translation of the canonical ORF on the same mRNA, independently of the dORF‐encoded peptide. This conserved and prevalent vertebrate post‐transcriptional regulatory mechanisms contrasts the translation‐repressing effects associated with upstream ORFs. Thousands of small dORFs are translated in human and zebrafish 3′ UTRs.dORF presence, but not the amino acid sequence, is conserved between human and zebrafish.Translation of dORFs enhances translation of the respective canonical ORFs. Graphical Abstract In contrast to upstream ORFs, translation of small open reading frames located in 3′ untranslated regions of mRNA promotes translation of the canonical ORF, independent of length or sequence of the encoded peptide. dORF (dpeaa)DE-He213 ribosome profiling (dpeaa)DE-He213 translation efficiency (dpeaa)DE-He213 Wright, Matthew verfasserin (orcid)0000-0002-6634-934X aut Gogol, Madelaine M verfasserin aut Bradford, William D verfasserin aut Zhang, Ning verfasserin aut Bazzini, Ariel A verfasserin (orcid)0000-0002-2251-5174 aut Enthalten in The EMBO Journal Nature Publishing Group UK, 2023 39(2020), 17 vom: 03. Aug. (DE-627)266022529 (DE-600)1467419-1 1460-2075 nnns volume:39 year:2020 number:17 day:03 month:08 https://dx.doi.org/10.15252/embj.2020104763 X:SPRINGER Resolving-System kostenfrei Volltext SYSFLAG_0 GBV_SPRINGER GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_72 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_161 GBV_ILN_168 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_211 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_252 GBV_ILN_266 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4029 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4116 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4155 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4311 GBV_ILN_4313 GBV_ILN_4314 GBV_ILN_4318 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4393 GBV_ILN_4598 GBV_ILN_4700 AR 39 2020 17 03 08 |
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10.15252/embj.2020104763 doi (DE-627)SPR058018522 (SPR)embj.2020104763-e DE-627 ger DE-627 rakwb eng Wu, Qiushuang verfasserin (orcid)0000-0002-9301-3630 aut Translation of small downstream ORFs enhances translation of canonical main open reading frames 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s) 2020 Abstract In addition to canonical open reading frames (ORFs), thousands of translated small ORFs (containing less than 100 codons) have been identified in untranslated mRNA regions (UTRs) across eukaryotes. Small ORFs in 5′ UTRs (upstream (u)ORFs) often repress translation of the canonical ORF within the same mRNA. However, the function of translated small ORFs in the 3′ UTRs (downstream (d)ORFs) is unknown. Contrary to uORFs, we find that translation of dORFs enhances translation of their corresponding canonical ORFs. This translation stimulatory effect of dORFs depends on the number of dORFs, but not the length or peptide they encode. We propose that dORFs represent a new, strong, and universal translation regulatory mechanism in vertebrates. Synopsis Translation of small downstream open reading frames (dORFs) in the 3′ untranslated region (UTR) enhances translation of the canonical ORF on the same mRNA, independently of the dORF‐encoded peptide. This conserved and prevalent vertebrate post‐transcriptional regulatory mechanisms contrasts the translation‐repressing effects associated with upstream ORFs. Thousands of small dORFs are translated in human and zebrafish 3′ UTRs.dORF presence, but not the amino acid sequence, is conserved between human and zebrafish.Translation of dORFs enhances translation of the respective canonical ORFs. Graphical Abstract In contrast to upstream ORFs, translation of small open reading frames located in 3′ untranslated regions of mRNA promotes translation of the canonical ORF, independent of length or sequence of the encoded peptide. dORF (dpeaa)DE-He213 ribosome profiling (dpeaa)DE-He213 translation efficiency (dpeaa)DE-He213 Wright, Matthew verfasserin (orcid)0000-0002-6634-934X aut Gogol, Madelaine M verfasserin aut Bradford, William D verfasserin aut Zhang, Ning verfasserin aut Bazzini, Ariel A verfasserin (orcid)0000-0002-2251-5174 aut Enthalten in The EMBO Journal Nature Publishing Group UK, 2023 39(2020), 17 vom: 03. Aug. (DE-627)266022529 (DE-600)1467419-1 1460-2075 nnns volume:39 year:2020 number:17 day:03 month:08 https://dx.doi.org/10.15252/embj.2020104763 X:SPRINGER Resolving-System kostenfrei Volltext SYSFLAG_0 GBV_SPRINGER GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_72 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_161 GBV_ILN_168 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_211 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_252 GBV_ILN_266 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4029 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4116 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4155 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4311 GBV_ILN_4313 GBV_ILN_4314 GBV_ILN_4318 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4393 GBV_ILN_4598 GBV_ILN_4700 AR 39 2020 17 03 08 |
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10.15252/embj.2020104763 doi (DE-627)SPR058018522 (SPR)embj.2020104763-e DE-627 ger DE-627 rakwb eng Wu, Qiushuang verfasserin (orcid)0000-0002-9301-3630 aut Translation of small downstream ORFs enhances translation of canonical main open reading frames 2020 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s) 2020 Abstract In addition to canonical open reading frames (ORFs), thousands of translated small ORFs (containing less than 100 codons) have been identified in untranslated mRNA regions (UTRs) across eukaryotes. Small ORFs in 5′ UTRs (upstream (u)ORFs) often repress translation of the canonical ORF within the same mRNA. However, the function of translated small ORFs in the 3′ UTRs (downstream (d)ORFs) is unknown. Contrary to uORFs, we find that translation of dORFs enhances translation of their corresponding canonical ORFs. This translation stimulatory effect of dORFs depends on the number of dORFs, but not the length or peptide they encode. We propose that dORFs represent a new, strong, and universal translation regulatory mechanism in vertebrates. Synopsis Translation of small downstream open reading frames (dORFs) in the 3′ untranslated region (UTR) enhances translation of the canonical ORF on the same mRNA, independently of the dORF‐encoded peptide. This conserved and prevalent vertebrate post‐transcriptional regulatory mechanisms contrasts the translation‐repressing effects associated with upstream ORFs. Thousands of small dORFs are translated in human and zebrafish 3′ UTRs.dORF presence, but not the amino acid sequence, is conserved between human and zebrafish.Translation of dORFs enhances translation of the respective canonical ORFs. Graphical Abstract In contrast to upstream ORFs, translation of small open reading frames located in 3′ untranslated regions of mRNA promotes translation of the canonical ORF, independent of length or sequence of the encoded peptide. dORF (dpeaa)DE-He213 ribosome profiling (dpeaa)DE-He213 translation efficiency (dpeaa)DE-He213 Wright, Matthew verfasserin (orcid)0000-0002-6634-934X aut Gogol, Madelaine M verfasserin aut Bradford, William D verfasserin aut Zhang, Ning verfasserin aut Bazzini, Ariel A verfasserin (orcid)0000-0002-2251-5174 aut Enthalten in The EMBO Journal Nature Publishing Group UK, 2023 39(2020), 17 vom: 03. Aug. (DE-627)266022529 (DE-600)1467419-1 1460-2075 nnns volume:39 year:2020 number:17 day:03 month:08 https://dx.doi.org/10.15252/embj.2020104763 X:SPRINGER Resolving-System kostenfrei Volltext SYSFLAG_0 GBV_SPRINGER GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_72 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_161 GBV_ILN_168 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_211 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_252 GBV_ILN_266 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4029 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4116 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4155 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4311 GBV_ILN_4313 GBV_ILN_4314 GBV_ILN_4318 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4393 GBV_ILN_4598 GBV_ILN_4700 AR 39 2020 17 03 08 |
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Enthalten in The EMBO Journal 39(2020), 17 vom: 03. Aug. volume:39 year:2020 number:17 day:03 month:08 |
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Wu, Qiushuang @@aut@@ Wright, Matthew @@aut@@ Gogol, Madelaine M @@aut@@ Bradford, William D @@aut@@ Zhang, Ning @@aut@@ Bazzini, Ariel A @@aut@@ |
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<?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>01000naa a22002652 4500</leader><controlfield tag="001">SPR058018522</controlfield><controlfield tag="003">DE-627</controlfield><controlfield tag="005">20241024065148.0</controlfield><controlfield tag="007">cr uuu---uuuuu</controlfield><controlfield tag="008">241024s2020 xx |||||o 00| ||eng c</controlfield><datafield tag="024" ind1="7" ind2=" "><subfield code="a">10.15252/embj.2020104763</subfield><subfield code="2">doi</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-627)SPR058018522</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(SPR)embj.2020104763-e</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-627</subfield><subfield code="b">ger</subfield><subfield code="c">DE-627</subfield><subfield code="e">rakwb</subfield></datafield><datafield tag="041" ind1=" " ind2=" "><subfield code="a">eng</subfield></datafield><datafield tag="100" ind1="1" ind2=" "><subfield code="a">Wu, Qiushuang</subfield><subfield code="e">verfasserin</subfield><subfield code="0">(orcid)0000-0002-9301-3630</subfield><subfield code="4">aut</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">Translation of small downstream ORFs enhances translation of canonical main open reading frames</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="c">2020</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="a">Text</subfield><subfield code="b">txt</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="a">Computermedien</subfield><subfield code="b">c</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="a">Online-Ressource</subfield><subfield code="b">cr</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="500" ind1=" " ind2=" "><subfield code="a">© The Author(s) 2020</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a">Abstract In addition to canonical open reading frames (ORFs), thousands of translated small ORFs (containing less than 100 codons) have been identified in untranslated mRNA regions (UTRs) across eukaryotes. Small ORFs in 5′ UTRs (upstream (u)ORFs) often repress translation of the canonical ORF within the same mRNA. However, the function of translated small ORFs in the 3′ UTRs (downstream (d)ORFs) is unknown. Contrary to uORFs, we find that translation of dORFs enhances translation of their corresponding canonical ORFs. This translation stimulatory effect of dORFs depends on the number of dORFs, but not the length or peptide they encode. We propose that dORFs represent a new, strong, and universal translation regulatory mechanism in vertebrates.</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a">Synopsis Translation of small downstream open reading frames (dORFs) in the 3′ untranslated region (UTR) enhances translation of the canonical ORF on the same mRNA, independently of the dORF‐encoded peptide. This conserved and prevalent vertebrate post‐transcriptional regulatory mechanisms contrasts the translation‐repressing effects associated with upstream ORFs. Thousands of small dORFs are translated in human and zebrafish 3′ UTRs.dORF presence, but not the amino acid sequence, is conserved between human and zebrafish.Translation of dORFs enhances translation of the respective canonical ORFs.</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a">Graphical Abstract In contrast to upstream ORFs, translation of small open reading frames located in 3′ untranslated regions of mRNA promotes translation of the canonical ORF, independent of length or sequence of the encoded peptide.</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">dORF</subfield><subfield code="7">(dpeaa)DE-He213</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">ribosome profiling</subfield><subfield code="7">(dpeaa)DE-He213</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">translation efficiency</subfield><subfield code="7">(dpeaa)DE-He213</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Wright, Matthew</subfield><subfield code="e">verfasserin</subfield><subfield code="0">(orcid)0000-0002-6634-934X</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Gogol, Madelaine M</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Bradford, William D</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Zhang, Ning</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Bazzini, Ariel A</subfield><subfield code="e">verfasserin</subfield><subfield code="0">(orcid)0000-0002-2251-5174</subfield><subfield code="4">aut</subfield></datafield><datafield tag="773" ind1="0" ind2="8"><subfield code="i">Enthalten in</subfield><subfield code="t">The EMBO Journal</subfield><subfield code="d">Nature Publishing Group UK, 2023</subfield><subfield code="g">39(2020), 17 vom: 03. 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Wu, Qiushuang |
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Wu, Qiushuang misc dORF misc ribosome profiling misc translation efficiency Translation of small downstream ORFs enhances translation of canonical main open reading frames |
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Translation of small downstream ORFs enhances translation of canonical main open reading frames dORF (dpeaa)DE-He213 ribosome profiling (dpeaa)DE-He213 translation efficiency (dpeaa)DE-He213 |
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misc dORF misc ribosome profiling misc translation efficiency |
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Translation of small downstream ORFs enhances translation of canonical main open reading frames |
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Translation of small downstream ORFs enhances translation of canonical main open reading frames |
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Wu, Qiushuang Wright, Matthew Gogol, Madelaine M Bradford, William D Zhang, Ning Bazzini, Ariel A |
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translation of small downstream orfs enhances translation of canonical main open reading frames |
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Translation of small downstream ORFs enhances translation of canonical main open reading frames |
| abstract |
Abstract In addition to canonical open reading frames (ORFs), thousands of translated small ORFs (containing less than 100 codons) have been identified in untranslated mRNA regions (UTRs) across eukaryotes. Small ORFs in 5′ UTRs (upstream (u)ORFs) often repress translation of the canonical ORF within the same mRNA. However, the function of translated small ORFs in the 3′ UTRs (downstream (d)ORFs) is unknown. Contrary to uORFs, we find that translation of dORFs enhances translation of their corresponding canonical ORFs. This translation stimulatory effect of dORFs depends on the number of dORFs, but not the length or peptide they encode. We propose that dORFs represent a new, strong, and universal translation regulatory mechanism in vertebrates. Synopsis Translation of small downstream open reading frames (dORFs) in the 3′ untranslated region (UTR) enhances translation of the canonical ORF on the same mRNA, independently of the dORF‐encoded peptide. This conserved and prevalent vertebrate post‐transcriptional regulatory mechanisms contrasts the translation‐repressing effects associated with upstream ORFs. Thousands of small dORFs are translated in human and zebrafish 3′ UTRs.dORF presence, but not the amino acid sequence, is conserved between human and zebrafish.Translation of dORFs enhances translation of the respective canonical ORFs. Graphical Abstract In contrast to upstream ORFs, translation of small open reading frames located in 3′ untranslated regions of mRNA promotes translation of the canonical ORF, independent of length or sequence of the encoded peptide. © The Author(s) 2020 |
| abstractGer |
Abstract In addition to canonical open reading frames (ORFs), thousands of translated small ORFs (containing less than 100 codons) have been identified in untranslated mRNA regions (UTRs) across eukaryotes. Small ORFs in 5′ UTRs (upstream (u)ORFs) often repress translation of the canonical ORF within the same mRNA. However, the function of translated small ORFs in the 3′ UTRs (downstream (d)ORFs) is unknown. Contrary to uORFs, we find that translation of dORFs enhances translation of their corresponding canonical ORFs. This translation stimulatory effect of dORFs depends on the number of dORFs, but not the length or peptide they encode. We propose that dORFs represent a new, strong, and universal translation regulatory mechanism in vertebrates. Synopsis Translation of small downstream open reading frames (dORFs) in the 3′ untranslated region (UTR) enhances translation of the canonical ORF on the same mRNA, independently of the dORF‐encoded peptide. This conserved and prevalent vertebrate post‐transcriptional regulatory mechanisms contrasts the translation‐repressing effects associated with upstream ORFs. Thousands of small dORFs are translated in human and zebrafish 3′ UTRs.dORF presence, but not the amino acid sequence, is conserved between human and zebrafish.Translation of dORFs enhances translation of the respective canonical ORFs. Graphical Abstract In contrast to upstream ORFs, translation of small open reading frames located in 3′ untranslated regions of mRNA promotes translation of the canonical ORF, independent of length or sequence of the encoded peptide. © The Author(s) 2020 |
| abstract_unstemmed |
Abstract In addition to canonical open reading frames (ORFs), thousands of translated small ORFs (containing less than 100 codons) have been identified in untranslated mRNA regions (UTRs) across eukaryotes. Small ORFs in 5′ UTRs (upstream (u)ORFs) often repress translation of the canonical ORF within the same mRNA. However, the function of translated small ORFs in the 3′ UTRs (downstream (d)ORFs) is unknown. Contrary to uORFs, we find that translation of dORFs enhances translation of their corresponding canonical ORFs. This translation stimulatory effect of dORFs depends on the number of dORFs, but not the length or peptide they encode. We propose that dORFs represent a new, strong, and universal translation regulatory mechanism in vertebrates. Synopsis Translation of small downstream open reading frames (dORFs) in the 3′ untranslated region (UTR) enhances translation of the canonical ORF on the same mRNA, independently of the dORF‐encoded peptide. This conserved and prevalent vertebrate post‐transcriptional regulatory mechanisms contrasts the translation‐repressing effects associated with upstream ORFs. Thousands of small dORFs are translated in human and zebrafish 3′ UTRs.dORF presence, but not the amino acid sequence, is conserved between human and zebrafish.Translation of dORFs enhances translation of the respective canonical ORFs. Graphical Abstract In contrast to upstream ORFs, translation of small open reading frames located in 3′ untranslated regions of mRNA promotes translation of the canonical ORF, independent of length or sequence of the encoded peptide. © The Author(s) 2020 |
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| container_issue |
17 |
| title_short |
Translation of small downstream ORFs enhances translation of canonical main open reading frames |
| url |
https://dx.doi.org/10.15252/embj.2020104763 |
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| author2 |
Wright, Matthew Gogol, Madelaine M Bradford, William D Zhang, Ning Bazzini, Ariel A |
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Wright, Matthew Gogol, Madelaine M Bradford, William D Zhang, Ning Bazzini, Ariel A |
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| doi_str |
10.15252/embj.2020104763 |
| up_date |
2024-10-24T04:56:32.725Z |
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| score |
7.4000845 |