Long‐distance mobile mRNA CAX3 modulates iron uptake and zinc compartmentalization

Abstract Iron deficiency in plants can lead to excessive absorption of zinc; however, important details of this mechanism have yet to be elucidated. Here, we report that MdCAX3 mRNA is transported from the leaf to the root, and that MdCAX3 is then activated by MdCXIP1. Suppression of MdCAX3 expressi...
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Autor*in:	Hao, Pengbo [verfasserIn] Lv, Xinmin [verfasserIn] Fu, Mengmeng [verfasserIn] Xu, Zhen [verfasserIn] Tian, Ji [verfasserIn] Wang, Yi [verfasserIn] Zhang, Xinzhong [verfasserIn] Xu, Xuefeng [verfasserIn] Wu, Ting [verfasserIn] Han, Zhenhai [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2022

Schlagwörter:	CAX3 Fe deficiency mobile mRNA PM H+‐ATPase Zn detoxification

Anmerkung:	© The Author(s) 2022

Übergeordnetes Werk:	Enthalten in: EMBO Reports - Nature Publishing Group UK, 2023, 23(2022), 5 vom: 07. März
Übergeordnetes Werk:	volume:23 ; year:2022 ; number:5 ; day:07 ; month:03

Links:	Volltext

DOI / URN:	10.15252/embr.202153698

Katalog-ID:	SPR058027491

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245	1	0	\|a Long‐distance mobile mRNA CAX3 modulates iron uptake and zinc compartmentalization
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520			\|a Abstract Iron deficiency in plants can lead to excessive absorption of zinc; however, important details of this mechanism have yet to be elucidated. Here, we report that MdCAX3 mRNA is transported from the leaf to the root, and that MdCAX3 is then activated by MdCXIP1. Suppression of MdCAX3 expression leads to an increase in the root apoplastic pH, which is associated with the iron deficiency response. Notably, overexpression of MdCAX3 does not affect the apoplastic pH in a MdCXIP1 loss‐of‐function Malus baccata (Mb) mutant that has a deletion in the MdCXIP1 promoter. This deletion in Mb weakens MdCXIP1 expression. Co‐expression of MdCAX3 and MdCXIP1 in Mb causes a decrease in the root apoplastic pH. Furthermore, suppressing MdCAX3 in Malus significantly reduces zinc vacuole compartmentalization. We also show that MdCAX3 activated by MdCXIP1 is not only involved in iron uptake, but also in regulating zinc detoxification by compartmentalizing zinc in vacuoles to avoid iron starvation‐induced zinc toxicity. Thus, mobile MdCAX3 mRNA is involved in the regulation of iron and zinc homeostasis in response to iron starvation.
520			\|a SYNOPSIS The long‐distance mobile mRNA MdCAX3 is upregulated in response to iron deficiency. It is transported from leaves to roots, where translated MdCAX3 is activated by the chaperone MdCXIP1 regulating iron and zinc homeostasis in response to iron starvation. In Malus grafts MdCAX3 mRNA is transported from the leaf to the root where its protein product is activated by MbCXIP1.Suppression of MdCAX3 leads to an increase in the root apoplastic pH, associated with Fe deficiency responses.Activated MdCAX3 promotes Zn compartmentalization into vacuoles to avoid Zn toxicity caused by Fe starvation.
520			\|a Graphical Abstract The long‐distance mobile mRNA MdCAX3 is upregulated in response to iron deficiency. It is transported from leaves to roots, where translated MdCAX3 is activated by the chaperone MdCXIP1 regulating iron and zinc homeostasis in response to iron starvation.
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publishDate	2022
allfields	10.15252/embr.202153698 doi (DE-627)SPR058027491 (SPR)embr.202153698-e DE-627 ger DE-627 rakwb eng Hao, Pengbo verfasserin aut Long‐distance mobile mRNA CAX3 modulates iron uptake and zinc compartmentalization 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s) 2022 Abstract Iron deficiency in plants can lead to excessive absorption of zinc; however, important details of this mechanism have yet to be elucidated. Here, we report that MdCAX3 mRNA is transported from the leaf to the root, and that MdCAX3 is then activated by MdCXIP1. Suppression of MdCAX3 expression leads to an increase in the root apoplastic pH, which is associated with the iron deficiency response. Notably, overexpression of MdCAX3 does not affect the apoplastic pH in a MdCXIP1 loss‐of‐function Malus baccata (Mb) mutant that has a deletion in the MdCXIP1 promoter. This deletion in Mb weakens MdCXIP1 expression. Co‐expression of MdCAX3 and MdCXIP1 in Mb causes a decrease in the root apoplastic pH. Furthermore, suppressing MdCAX3 in Malus significantly reduces zinc vacuole compartmentalization. We also show that MdCAX3 activated by MdCXIP1 is not only involved in iron uptake, but also in regulating zinc detoxification by compartmentalizing zinc in vacuoles to avoid iron starvation‐induced zinc toxicity. Thus, mobile MdCAX3 mRNA is involved in the regulation of iron and zinc homeostasis in response to iron starvation. SYNOPSIS The long‐distance mobile mRNA MdCAX3 is upregulated in response to iron deficiency. It is transported from leaves to roots, where translated MdCAX3 is activated by the chaperone MdCXIP1 regulating iron and zinc homeostasis in response to iron starvation. In Malus grafts MdCAX3 mRNA is transported from the leaf to the root where its protein product is activated by MbCXIP1.Suppression of MdCAX3 leads to an increase in the root apoplastic pH, associated with Fe deficiency responses.Activated MdCAX3 promotes Zn compartmentalization into vacuoles to avoid Zn toxicity caused by Fe starvation. Graphical Abstract The long‐distance mobile mRNA MdCAX3 is upregulated in response to iron deficiency. It is transported from leaves to roots, where translated MdCAX3 is activated by the chaperone MdCXIP1 regulating iron and zinc homeostasis in response to iron starvation. CAX3 (dpeaa)DE-He213 Fe deficiency (dpeaa)DE-He213 mobile mRNA (dpeaa)DE-He213 PM H+‐ATPase (dpeaa)DE-He213 Zn detoxification (dpeaa)DE-He213 Lv, Xinmin verfasserin aut Fu, Mengmeng verfasserin aut Xu, Zhen verfasserin aut Tian, Ji verfasserin aut Wang, Yi verfasserin (orcid)0000-0002-6303-385X aut Zhang, Xinzhong verfasserin aut Xu, Xuefeng verfasserin aut Wu, Ting verfasserin (orcid)0000-0003-1316-6563 aut Han, Zhenhai verfasserin (orcid)0000-0003-3759-1046 aut Enthalten in EMBO Reports Nature Publishing Group UK, 2023 23(2022), 5 vom: 07. März (DE-627)320645622 (DE-600)2025376-X 1469-3178 nnns volume:23 year:2022 number:5 day:07 month:03 https://dx.doi.org/10.15252/embr.202153698 X:SPRINGER Resolving-System lizenzpflichtig Volltext SYSFLAG_0 GBV_SPRINGER GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_72 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_161 GBV_ILN_168 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_211 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_252 GBV_ILN_266 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4029 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4116 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4155 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4311 GBV_ILN_4313 GBV_ILN_4314 GBV_ILN_4318 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4393 GBV_ILN_4598 GBV_ILN_4700 AR 23 2022 5 07 03
spelling	10.15252/embr.202153698 doi (DE-627)SPR058027491 (SPR)embr.202153698-e DE-627 ger DE-627 rakwb eng Hao, Pengbo verfasserin aut Long‐distance mobile mRNA CAX3 modulates iron uptake and zinc compartmentalization 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s) 2022 Abstract Iron deficiency in plants can lead to excessive absorption of zinc; however, important details of this mechanism have yet to be elucidated. Here, we report that MdCAX3 mRNA is transported from the leaf to the root, and that MdCAX3 is then activated by MdCXIP1. Suppression of MdCAX3 expression leads to an increase in the root apoplastic pH, which is associated with the iron deficiency response. Notably, overexpression of MdCAX3 does not affect the apoplastic pH in a MdCXIP1 loss‐of‐function Malus baccata (Mb) mutant that has a deletion in the MdCXIP1 promoter. This deletion in Mb weakens MdCXIP1 expression. Co‐expression of MdCAX3 and MdCXIP1 in Mb causes a decrease in the root apoplastic pH. Furthermore, suppressing MdCAX3 in Malus significantly reduces zinc vacuole compartmentalization. We also show that MdCAX3 activated by MdCXIP1 is not only involved in iron uptake, but also in regulating zinc detoxification by compartmentalizing zinc in vacuoles to avoid iron starvation‐induced zinc toxicity. Thus, mobile MdCAX3 mRNA is involved in the regulation of iron and zinc homeostasis in response to iron starvation. SYNOPSIS The long‐distance mobile mRNA MdCAX3 is upregulated in response to iron deficiency. It is transported from leaves to roots, where translated MdCAX3 is activated by the chaperone MdCXIP1 regulating iron and zinc homeostasis in response to iron starvation. In Malus grafts MdCAX3 mRNA is transported from the leaf to the root where its protein product is activated by MbCXIP1.Suppression of MdCAX3 leads to an increase in the root apoplastic pH, associated with Fe deficiency responses.Activated MdCAX3 promotes Zn compartmentalization into vacuoles to avoid Zn toxicity caused by Fe starvation. Graphical Abstract The long‐distance mobile mRNA MdCAX3 is upregulated in response to iron deficiency. It is transported from leaves to roots, where translated MdCAX3 is activated by the chaperone MdCXIP1 regulating iron and zinc homeostasis in response to iron starvation. CAX3 (dpeaa)DE-He213 Fe deficiency (dpeaa)DE-He213 mobile mRNA (dpeaa)DE-He213 PM H+‐ATPase (dpeaa)DE-He213 Zn detoxification (dpeaa)DE-He213 Lv, Xinmin verfasserin aut Fu, Mengmeng verfasserin aut Xu, Zhen verfasserin aut Tian, Ji verfasserin aut Wang, Yi verfasserin (orcid)0000-0002-6303-385X aut Zhang, Xinzhong verfasserin aut Xu, Xuefeng verfasserin aut Wu, Ting verfasserin (orcid)0000-0003-1316-6563 aut Han, Zhenhai verfasserin (orcid)0000-0003-3759-1046 aut Enthalten in EMBO Reports Nature Publishing Group UK, 2023 23(2022), 5 vom: 07. März (DE-627)320645622 (DE-600)2025376-X 1469-3178 nnns volume:23 year:2022 number:5 day:07 month:03 https://dx.doi.org/10.15252/embr.202153698 X:SPRINGER Resolving-System lizenzpflichtig Volltext SYSFLAG_0 GBV_SPRINGER GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_72 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_161 GBV_ILN_168 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_211 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_252 GBV_ILN_266 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4029 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4116 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4155 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4311 GBV_ILN_4313 GBV_ILN_4314 GBV_ILN_4318 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4393 GBV_ILN_4598 GBV_ILN_4700 AR 23 2022 5 07 03
allfields_unstemmed	10.15252/embr.202153698 doi (DE-627)SPR058027491 (SPR)embr.202153698-e DE-627 ger DE-627 rakwb eng Hao, Pengbo verfasserin aut Long‐distance mobile mRNA CAX3 modulates iron uptake and zinc compartmentalization 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s) 2022 Abstract Iron deficiency in plants can lead to excessive absorption of zinc; however, important details of this mechanism have yet to be elucidated. Here, we report that MdCAX3 mRNA is transported from the leaf to the root, and that MdCAX3 is then activated by MdCXIP1. Suppression of MdCAX3 expression leads to an increase in the root apoplastic pH, which is associated with the iron deficiency response. Notably, overexpression of MdCAX3 does not affect the apoplastic pH in a MdCXIP1 loss‐of‐function Malus baccata (Mb) mutant that has a deletion in the MdCXIP1 promoter. This deletion in Mb weakens MdCXIP1 expression. Co‐expression of MdCAX3 and MdCXIP1 in Mb causes a decrease in the root apoplastic pH. Furthermore, suppressing MdCAX3 in Malus significantly reduces zinc vacuole compartmentalization. We also show that MdCAX3 activated by MdCXIP1 is not only involved in iron uptake, but also in regulating zinc detoxification by compartmentalizing zinc in vacuoles to avoid iron starvation‐induced zinc toxicity. Thus, mobile MdCAX3 mRNA is involved in the regulation of iron and zinc homeostasis in response to iron starvation. SYNOPSIS The long‐distance mobile mRNA MdCAX3 is upregulated in response to iron deficiency. It is transported from leaves to roots, where translated MdCAX3 is activated by the chaperone MdCXIP1 regulating iron and zinc homeostasis in response to iron starvation. In Malus grafts MdCAX3 mRNA is transported from the leaf to the root where its protein product is activated by MbCXIP1.Suppression of MdCAX3 leads to an increase in the root apoplastic pH, associated with Fe deficiency responses.Activated MdCAX3 promotes Zn compartmentalization into vacuoles to avoid Zn toxicity caused by Fe starvation. Graphical Abstract The long‐distance mobile mRNA MdCAX3 is upregulated in response to iron deficiency. It is transported from leaves to roots, where translated MdCAX3 is activated by the chaperone MdCXIP1 regulating iron and zinc homeostasis in response to iron starvation. CAX3 (dpeaa)DE-He213 Fe deficiency (dpeaa)DE-He213 mobile mRNA (dpeaa)DE-He213 PM H+‐ATPase (dpeaa)DE-He213 Zn detoxification (dpeaa)DE-He213 Lv, Xinmin verfasserin aut Fu, Mengmeng verfasserin aut Xu, Zhen verfasserin aut Tian, Ji verfasserin aut Wang, Yi verfasserin (orcid)0000-0002-6303-385X aut Zhang, Xinzhong verfasserin aut Xu, Xuefeng verfasserin aut Wu, Ting verfasserin (orcid)0000-0003-1316-6563 aut Han, Zhenhai verfasserin (orcid)0000-0003-3759-1046 aut Enthalten in EMBO Reports Nature Publishing Group UK, 2023 23(2022), 5 vom: 07. März (DE-627)320645622 (DE-600)2025376-X 1469-3178 nnns volume:23 year:2022 number:5 day:07 month:03 https://dx.doi.org/10.15252/embr.202153698 X:SPRINGER Resolving-System lizenzpflichtig Volltext SYSFLAG_0 GBV_SPRINGER GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_72 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_161 GBV_ILN_168 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_211 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_252 GBV_ILN_266 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4029 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4116 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4155 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4311 GBV_ILN_4313 GBV_ILN_4314 GBV_ILN_4318 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4393 GBV_ILN_4598 GBV_ILN_4700 AR 23 2022 5 07 03
allfieldsGer	10.15252/embr.202153698 doi (DE-627)SPR058027491 (SPR)embr.202153698-e DE-627 ger DE-627 rakwb eng Hao, Pengbo verfasserin aut Long‐distance mobile mRNA CAX3 modulates iron uptake and zinc compartmentalization 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s) 2022 Abstract Iron deficiency in plants can lead to excessive absorption of zinc; however, important details of this mechanism have yet to be elucidated. Here, we report that MdCAX3 mRNA is transported from the leaf to the root, and that MdCAX3 is then activated by MdCXIP1. Suppression of MdCAX3 expression leads to an increase in the root apoplastic pH, which is associated with the iron deficiency response. Notably, overexpression of MdCAX3 does not affect the apoplastic pH in a MdCXIP1 loss‐of‐function Malus baccata (Mb) mutant that has a deletion in the MdCXIP1 promoter. This deletion in Mb weakens MdCXIP1 expression. Co‐expression of MdCAX3 and MdCXIP1 in Mb causes a decrease in the root apoplastic pH. Furthermore, suppressing MdCAX3 in Malus significantly reduces zinc vacuole compartmentalization. We also show that MdCAX3 activated by MdCXIP1 is not only involved in iron uptake, but also in regulating zinc detoxification by compartmentalizing zinc in vacuoles to avoid iron starvation‐induced zinc toxicity. Thus, mobile MdCAX3 mRNA is involved in the regulation of iron and zinc homeostasis in response to iron starvation. SYNOPSIS The long‐distance mobile mRNA MdCAX3 is upregulated in response to iron deficiency. It is transported from leaves to roots, where translated MdCAX3 is activated by the chaperone MdCXIP1 regulating iron and zinc homeostasis in response to iron starvation. In Malus grafts MdCAX3 mRNA is transported from the leaf to the root where its protein product is activated by MbCXIP1.Suppression of MdCAX3 leads to an increase in the root apoplastic pH, associated with Fe deficiency responses.Activated MdCAX3 promotes Zn compartmentalization into vacuoles to avoid Zn toxicity caused by Fe starvation. Graphical Abstract The long‐distance mobile mRNA MdCAX3 is upregulated in response to iron deficiency. It is transported from leaves to roots, where translated MdCAX3 is activated by the chaperone MdCXIP1 regulating iron and zinc homeostasis in response to iron starvation. CAX3 (dpeaa)DE-He213 Fe deficiency (dpeaa)DE-He213 mobile mRNA (dpeaa)DE-He213 PM H+‐ATPase (dpeaa)DE-He213 Zn detoxification (dpeaa)DE-He213 Lv, Xinmin verfasserin aut Fu, Mengmeng verfasserin aut Xu, Zhen verfasserin aut Tian, Ji verfasserin aut Wang, Yi verfasserin (orcid)0000-0002-6303-385X aut Zhang, Xinzhong verfasserin aut Xu, Xuefeng verfasserin aut Wu, Ting verfasserin (orcid)0000-0003-1316-6563 aut Han, Zhenhai verfasserin (orcid)0000-0003-3759-1046 aut Enthalten in EMBO Reports Nature Publishing Group UK, 2023 23(2022), 5 vom: 07. März (DE-627)320645622 (DE-600)2025376-X 1469-3178 nnns volume:23 year:2022 number:5 day:07 month:03 https://dx.doi.org/10.15252/embr.202153698 X:SPRINGER Resolving-System lizenzpflichtig Volltext SYSFLAG_0 GBV_SPRINGER GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_72 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_161 GBV_ILN_168 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_211 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_252 GBV_ILN_266 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4029 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4116 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4155 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4311 GBV_ILN_4313 GBV_ILN_4314 GBV_ILN_4318 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4393 GBV_ILN_4598 GBV_ILN_4700 AR 23 2022 5 07 03
allfieldsSound	10.15252/embr.202153698 doi (DE-627)SPR058027491 (SPR)embr.202153698-e DE-627 ger DE-627 rakwb eng Hao, Pengbo verfasserin aut Long‐distance mobile mRNA CAX3 modulates iron uptake and zinc compartmentalization 2022 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s) 2022 Abstract Iron deficiency in plants can lead to excessive absorption of zinc; however, important details of this mechanism have yet to be elucidated. Here, we report that MdCAX3 mRNA is transported from the leaf to the root, and that MdCAX3 is then activated by MdCXIP1. Suppression of MdCAX3 expression leads to an increase in the root apoplastic pH, which is associated with the iron deficiency response. Notably, overexpression of MdCAX3 does not affect the apoplastic pH in a MdCXIP1 loss‐of‐function Malus baccata (Mb) mutant that has a deletion in the MdCXIP1 promoter. This deletion in Mb weakens MdCXIP1 expression. Co‐expression of MdCAX3 and MdCXIP1 in Mb causes a decrease in the root apoplastic pH. Furthermore, suppressing MdCAX3 in Malus significantly reduces zinc vacuole compartmentalization. We also show that MdCAX3 activated by MdCXIP1 is not only involved in iron uptake, but also in regulating zinc detoxification by compartmentalizing zinc in vacuoles to avoid iron starvation‐induced zinc toxicity. Thus, mobile MdCAX3 mRNA is involved in the regulation of iron and zinc homeostasis in response to iron starvation. SYNOPSIS The long‐distance mobile mRNA MdCAX3 is upregulated in response to iron deficiency. It is transported from leaves to roots, where translated MdCAX3 is activated by the chaperone MdCXIP1 regulating iron and zinc homeostasis in response to iron starvation. In Malus grafts MdCAX3 mRNA is transported from the leaf to the root where its protein product is activated by MbCXIP1.Suppression of MdCAX3 leads to an increase in the root apoplastic pH, associated with Fe deficiency responses.Activated MdCAX3 promotes Zn compartmentalization into vacuoles to avoid Zn toxicity caused by Fe starvation. Graphical Abstract The long‐distance mobile mRNA MdCAX3 is upregulated in response to iron deficiency. It is transported from leaves to roots, where translated MdCAX3 is activated by the chaperone MdCXIP1 regulating iron and zinc homeostasis in response to iron starvation. CAX3 (dpeaa)DE-He213 Fe deficiency (dpeaa)DE-He213 mobile mRNA (dpeaa)DE-He213 PM H+‐ATPase (dpeaa)DE-He213 Zn detoxification (dpeaa)DE-He213 Lv, Xinmin verfasserin aut Fu, Mengmeng verfasserin aut Xu, Zhen verfasserin aut Tian, Ji verfasserin aut Wang, Yi verfasserin (orcid)0000-0002-6303-385X aut Zhang, Xinzhong verfasserin aut Xu, Xuefeng verfasserin aut Wu, Ting verfasserin (orcid)0000-0003-1316-6563 aut Han, Zhenhai verfasserin (orcid)0000-0003-3759-1046 aut Enthalten in EMBO Reports Nature Publishing Group UK, 2023 23(2022), 5 vom: 07. März (DE-627)320645622 (DE-600)2025376-X 1469-3178 nnns volume:23 year:2022 number:5 day:07 month:03 https://dx.doi.org/10.15252/embr.202153698 X:SPRINGER Resolving-System lizenzpflichtig Volltext SYSFLAG_0 GBV_SPRINGER GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_72 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_161 GBV_ILN_168 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_211 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_252 GBV_ILN_266 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4029 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4116 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4155 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4311 GBV_ILN_4313 GBV_ILN_4314 GBV_ILN_4318 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4393 GBV_ILN_4598 GBV_ILN_4700 AR 23 2022 5 07 03
language	English
source	Enthalten in EMBO Reports 23(2022), 5 vom: 07. März volume:23 year:2022 number:5 day:07 month:03
sourceStr	Enthalten in EMBO Reports 23(2022), 5 vom: 07. März volume:23 year:2022 number:5 day:07 month:03
format_phy_str_mv	Article
institution	findex.gbv.de
topic_facet	CAX3 Fe deficiency mobile mRNA PM H+‐ATPase Zn detoxification
isfreeaccess_bool	false
container_title	EMBO Reports
authorswithroles_txt_mv	Hao, Pengbo @@aut@@ Lv, Xinmin @@aut@@ Fu, Mengmeng @@aut@@ Xu, Zhen @@aut@@ Tian, Ji @@aut@@ Wang, Yi @@aut@@ Zhang, Xinzhong @@aut@@ Xu, Xuefeng @@aut@@ Wu, Ting @@aut@@ Han, Zhenhai @@aut@@
publishDateDaySort_date	2022-03-07T00:00:00Z
hierarchy_top_id	320645622
id	SPR058027491
language_de	englisch
fullrecord	<?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>01000naa a22002652 4500</leader><controlfield tag="001">SPR058027491</controlfield><controlfield tag="003">DE-627</controlfield><controlfield tag="005">20241024065216.0</controlfield><controlfield tag="007">cr uuu---uuuuu</controlfield><controlfield tag="008">241024s2022 xx \|\|\|\|\|o 00\| \|\|eng c</controlfield><datafield tag="024" ind1="7" ind2=" "><subfield code="a">10.15252/embr.202153698</subfield><subfield code="2">doi</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-627)SPR058027491</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(SPR)embr.202153698-e</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-627</subfield><subfield code="b">ger</subfield><subfield code="c">DE-627</subfield><subfield code="e">rakwb</subfield></datafield><datafield tag="041" ind1=" " ind2=" "><subfield code="a">eng</subfield></datafield><datafield tag="100" ind1="1" ind2=" "><subfield code="a">Hao, Pengbo</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">Long‐distance mobile mRNA CAX3 modulates iron uptake and zinc compartmentalization</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="c">2022</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="a">Text</subfield><subfield code="b">txt</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="a">Computermedien</subfield><subfield code="b">c</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="a">Online-Ressource</subfield><subfield code="b">cr</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="500" ind1=" " ind2=" "><subfield code="a">© The Author(s) 2022</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a">Abstract Iron deficiency in plants can lead to excessive absorption of zinc; however, important details of this mechanism have yet to be elucidated. Here, we report that MdCAX3 mRNA is transported from the leaf to the root, and that MdCAX3 is then activated by MdCXIP1. Suppression of MdCAX3 expression leads to an increase in the root apoplastic pH, which is associated with the iron deficiency response. Notably, overexpression of MdCAX3 does not affect the apoplastic pH in a MdCXIP1 loss‐of‐function Malus baccata (Mb) mutant that has a deletion in the MdCXIP1 promoter. This deletion in Mb weakens MdCXIP1 expression. Co‐expression of MdCAX3 and MdCXIP1 in Mb causes a decrease in the root apoplastic pH. Furthermore, suppressing MdCAX3 in Malus significantly reduces zinc vacuole compartmentalization. We also show that MdCAX3 activated by MdCXIP1 is not only involved in iron uptake, but also in regulating zinc detoxification by compartmentalizing zinc in vacuoles to avoid iron starvation‐induced zinc toxicity. Thus, mobile MdCAX3 mRNA is involved in the regulation of iron and zinc homeostasis in response to iron starvation.</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a">SYNOPSIS The long‐distance mobile mRNA MdCAX3 is upregulated in response to iron deficiency. It is transported from leaves to roots, where translated MdCAX3 is activated by the chaperone MdCXIP1 regulating iron and zinc homeostasis in response to iron starvation. In Malus grafts MdCAX3 mRNA is transported from the leaf to the root where its protein product is activated by MbCXIP1.Suppression of MdCAX3 leads to an increase in the root apoplastic pH, associated with Fe deficiency responses.Activated MdCAX3 promotes Zn compartmentalization into vacuoles to avoid Zn toxicity caused by Fe starvation.</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a">Graphical Abstract The long‐distance mobile mRNA MdCAX3 is upregulated in response to iron deficiency. 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author	Hao, Pengbo
spellingShingle	Hao, Pengbo misc CAX3 misc Fe deficiency misc mobile mRNA misc PM H+‐ATPase misc Zn detoxification Long‐distance mobile mRNA CAX3 modulates iron uptake and zinc compartmentalization
authorStr	Hao, Pengbo
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topic_title	Long‐distance mobile mRNA CAX3 modulates iron uptake and zinc compartmentalization CAX3 (dpeaa)DE-He213 Fe deficiency (dpeaa)DE-He213 mobile mRNA (dpeaa)DE-He213 PM H+‐ATPase (dpeaa)DE-He213 Zn detoxification (dpeaa)DE-He213
topic	misc CAX3 misc Fe deficiency misc mobile mRNA misc PM H+‐ATPase misc Zn detoxification
topic_unstemmed	misc CAX3 misc Fe deficiency misc mobile mRNA misc PM H+‐ATPase misc Zn detoxification
topic_browse	misc CAX3 misc Fe deficiency misc mobile mRNA misc PM H+‐ATPase misc Zn detoxification
format_facet	Elektronische Aufsätze Aufsätze Elektronische Ressource
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title	Long‐distance mobile mRNA CAX3 modulates iron uptake and zinc compartmentalization
ctrlnum	(DE-627)SPR058027491 (SPR)embr.202153698-e
title_full	Long‐distance mobile mRNA CAX3 modulates iron uptake and zinc compartmentalization
author_sort	Hao, Pengbo
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author_browse	Hao, Pengbo Lv, Xinmin Fu, Mengmeng Xu, Zhen Tian, Ji Wang, Yi Zhang, Xinzhong Xu, Xuefeng Wu, Ting Han, Zhenhai
container_volume	23
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author-letter	Hao, Pengbo
doi_str_mv	10.15252/embr.202153698
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title_sort	long‐distance mobile mrna cax3 modulates iron uptake and zinc compartmentalization
title_auth	Long‐distance mobile mRNA CAX3 modulates iron uptake and zinc compartmentalization
abstract	Abstract Iron deficiency in plants can lead to excessive absorption of zinc; however, important details of this mechanism have yet to be elucidated. Here, we report that MdCAX3 mRNA is transported from the leaf to the root, and that MdCAX3 is then activated by MdCXIP1. Suppression of MdCAX3 expression leads to an increase in the root apoplastic pH, which is associated with the iron deficiency response. Notably, overexpression of MdCAX3 does not affect the apoplastic pH in a MdCXIP1 loss‐of‐function Malus baccata (Mb) mutant that has a deletion in the MdCXIP1 promoter. This deletion in Mb weakens MdCXIP1 expression. Co‐expression of MdCAX3 and MdCXIP1 in Mb causes a decrease in the root apoplastic pH. Furthermore, suppressing MdCAX3 in Malus significantly reduces zinc vacuole compartmentalization. We also show that MdCAX3 activated by MdCXIP1 is not only involved in iron uptake, but also in regulating zinc detoxification by compartmentalizing zinc in vacuoles to avoid iron starvation‐induced zinc toxicity. Thus, mobile MdCAX3 mRNA is involved in the regulation of iron and zinc homeostasis in response to iron starvation. SYNOPSIS The long‐distance mobile mRNA MdCAX3 is upregulated in response to iron deficiency. It is transported from leaves to roots, where translated MdCAX3 is activated by the chaperone MdCXIP1 regulating iron and zinc homeostasis in response to iron starvation. In Malus grafts MdCAX3 mRNA is transported from the leaf to the root where its protein product is activated by MbCXIP1.Suppression of MdCAX3 leads to an increase in the root apoplastic pH, associated with Fe deficiency responses.Activated MdCAX3 promotes Zn compartmentalization into vacuoles to avoid Zn toxicity caused by Fe starvation. Graphical Abstract The long‐distance mobile mRNA MdCAX3 is upregulated in response to iron deficiency. It is transported from leaves to roots, where translated MdCAX3 is activated by the chaperone MdCXIP1 regulating iron and zinc homeostasis in response to iron starvation. © The Author(s) 2022
abstractGer	Abstract Iron deficiency in plants can lead to excessive absorption of zinc; however, important details of this mechanism have yet to be elucidated. Here, we report that MdCAX3 mRNA is transported from the leaf to the root, and that MdCAX3 is then activated by MdCXIP1. Suppression of MdCAX3 expression leads to an increase in the root apoplastic pH, which is associated with the iron deficiency response. Notably, overexpression of MdCAX3 does not affect the apoplastic pH in a MdCXIP1 loss‐of‐function Malus baccata (Mb) mutant that has a deletion in the MdCXIP1 promoter. This deletion in Mb weakens MdCXIP1 expression. Co‐expression of MdCAX3 and MdCXIP1 in Mb causes a decrease in the root apoplastic pH. Furthermore, suppressing MdCAX3 in Malus significantly reduces zinc vacuole compartmentalization. We also show that MdCAX3 activated by MdCXIP1 is not only involved in iron uptake, but also in regulating zinc detoxification by compartmentalizing zinc in vacuoles to avoid iron starvation‐induced zinc toxicity. Thus, mobile MdCAX3 mRNA is involved in the regulation of iron and zinc homeostasis in response to iron starvation. SYNOPSIS The long‐distance mobile mRNA MdCAX3 is upregulated in response to iron deficiency. It is transported from leaves to roots, where translated MdCAX3 is activated by the chaperone MdCXIP1 regulating iron and zinc homeostasis in response to iron starvation. In Malus grafts MdCAX3 mRNA is transported from the leaf to the root where its protein product is activated by MbCXIP1.Suppression of MdCAX3 leads to an increase in the root apoplastic pH, associated with Fe deficiency responses.Activated MdCAX3 promotes Zn compartmentalization into vacuoles to avoid Zn toxicity caused by Fe starvation. Graphical Abstract The long‐distance mobile mRNA MdCAX3 is upregulated in response to iron deficiency. It is transported from leaves to roots, where translated MdCAX3 is activated by the chaperone MdCXIP1 regulating iron and zinc homeostasis in response to iron starvation. © The Author(s) 2022
abstract_unstemmed	Abstract Iron deficiency in plants can lead to excessive absorption of zinc; however, important details of this mechanism have yet to be elucidated. Here, we report that MdCAX3 mRNA is transported from the leaf to the root, and that MdCAX3 is then activated by MdCXIP1. Suppression of MdCAX3 expression leads to an increase in the root apoplastic pH, which is associated with the iron deficiency response. Notably, overexpression of MdCAX3 does not affect the apoplastic pH in a MdCXIP1 loss‐of‐function Malus baccata (Mb) mutant that has a deletion in the MdCXIP1 promoter. This deletion in Mb weakens MdCXIP1 expression. Co‐expression of MdCAX3 and MdCXIP1 in Mb causes a decrease in the root apoplastic pH. Furthermore, suppressing MdCAX3 in Malus significantly reduces zinc vacuole compartmentalization. We also show that MdCAX3 activated by MdCXIP1 is not only involved in iron uptake, but also in regulating zinc detoxification by compartmentalizing zinc in vacuoles to avoid iron starvation‐induced zinc toxicity. Thus, mobile MdCAX3 mRNA is involved in the regulation of iron and zinc homeostasis in response to iron starvation. SYNOPSIS The long‐distance mobile mRNA MdCAX3 is upregulated in response to iron deficiency. It is transported from leaves to roots, where translated MdCAX3 is activated by the chaperone MdCXIP1 regulating iron and zinc homeostasis in response to iron starvation. In Malus grafts MdCAX3 mRNA is transported from the leaf to the root where its protein product is activated by MbCXIP1.Suppression of MdCAX3 leads to an increase in the root apoplastic pH, associated with Fe deficiency responses.Activated MdCAX3 promotes Zn compartmentalization into vacuoles to avoid Zn toxicity caused by Fe starvation. Graphical Abstract The long‐distance mobile mRNA MdCAX3 is upregulated in response to iron deficiency. It is transported from leaves to roots, where translated MdCAX3 is activated by the chaperone MdCXIP1 regulating iron and zinc homeostasis in response to iron starvation. © The Author(s) 2022
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container_issue	5
title_short	Long‐distance mobile mRNA CAX3 modulates iron uptake and zinc compartmentalization
url	https://dx.doi.org/10.15252/embr.202153698
remote_bool	true
author2	Lv, Xinmin Fu, Mengmeng Xu, Zhen Tian, Ji Wang, Yi Zhang, Xinzhong Xu, Xuefeng Wu, Ting Han, Zhenhai
author2Str	Lv, Xinmin Fu, Mengmeng Xu, Zhen Tian, Ji Wang, Yi Zhang, Xinzhong Xu, Xuefeng Wu, Ting Han, Zhenhai
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doi_str	10.15252/embr.202153698
up_date	2024-10-24T04:56:09.732Z
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Long‐distance mobile mRNA CAX3 modulates iron uptake and zinc compartmentalization

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