Single‐cell transcriptomics reveals distinct inflammation‐induced microglia signatures
Abstract Microglia are specialized parenchymal‐resident phagocytes of the central nervous system (CNS) that actively support, defend and modulate the neural environment. Dysfunctional microglial responses are thought to worsen CNS diseases; nevertheless, their impact during neuroinflammatory process...
Ausführliche Beschreibung
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| Autor*in: |
Sousa, Carole [verfasserIn] Golebiewska, Anna [verfasserIn] Poovathingal, Suresh K [verfasserIn] Kaoma, Tony [verfasserIn] Pires‐Afonso, Yolanda [verfasserIn] Martina, Silvia [verfasserIn] Coowar, Djalil [verfasserIn] Azuaje, Francisco [verfasserIn] Skupin, Alexander [verfasserIn] Balling, Rudi [verfasserIn] Biber, Knut [verfasserIn] Niclou, Simone P [verfasserIn] Michelucci, Alessandro [verfasserIn] |
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| Format: |
E-Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
2018 |
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| Schlagwörter: |
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| Anmerkung: |
© The Author(s) 2018 |
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| Übergeordnetes Werk: |
Enthalten in: EMBO Reports - Nature Publishing Group UK, 2023, 19(2018), 11 vom: 11. Sept. |
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| Übergeordnetes Werk: |
volume:19 ; year:2018 ; number:11 ; day:11 ; month:09 |
| Links: |
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| DOI / URN: |
10.15252/embr.201846171 |
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| Katalog-ID: |
SPR058085351 |
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| 100 | 1 | |a Sousa, Carole |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Single‐cell transcriptomics reveals distinct inflammation‐induced microglia signatures |
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| 520 | |a Abstract Microglia are specialized parenchymal‐resident phagocytes of the central nervous system (CNS) that actively support, defend and modulate the neural environment. Dysfunctional microglial responses are thought to worsen CNS diseases; nevertheless, their impact during neuroinflammatory processes remains largely obscure. Here, using a combination of single‐cell RNA sequencing and multicolour flow cytometry, we comprehensively profile microglia in the brain of lipopolysaccharide (LPS)‐injected mice. By excluding the contribution of other immune CNS‐resident and peripheral cells, we show that microglia isolated from LPS‐injected mice display a global downregulation of their homeostatic signature together with an upregulation of inflammatory genes. Notably, we identify distinct microglial activated profiles under inflammatory conditions, which greatly differ from neurodegenerative disease‐associated profiles. These results provide insights into microglial heterogeneity and establish a resource for the identification of specific phenotypes in CNS disorders, such as neuroinflammatory and neurodegenerative diseases. | ||
| 520 | |a Synopsis Using single‐cell transcriptomics and multicolour flow cytometry this study presents comprehensive profiles of microglia in LPS‐injected mice, providing insight into microglia heterogeneity, and establishing a resource for the identification of specific phenotypes in CNS disorders. Microglia homeostatic signatures are mainly lost upon acute systemic inflammation.Inflammation‐induced microglia segregate into two distinct reactive states.Inflammation‐induced microglia signatures are distinct from neurodegenerative disease‐associated profiles. | ||
| 520 | |a Graphical Abstract Using single‐cell transcriptomics and multicolour flow cytometry this study presents comprehensive profiles of microglia in LPS‐injected mice, providing insight into microglia heterogeneity, and establishing a resource for the identification of specific phenotypes in CNS disorders. | ||
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| 650 | 4 | |a single‐cell RNA‐seq |7 (dpeaa)DE-He213 | |
| 700 | 1 | |a Golebiewska, Anna |e verfasserin |4 aut | |
| 700 | 1 | |a Poovathingal, Suresh K |e verfasserin |4 aut | |
| 700 | 1 | |a Kaoma, Tony |e verfasserin |4 aut | |
| 700 | 1 | |a Pires‐Afonso, Yolanda |e verfasserin |4 aut | |
| 700 | 1 | |a Martina, Silvia |e verfasserin |4 aut | |
| 700 | 1 | |a Coowar, Djalil |e verfasserin |4 aut | |
| 700 | 1 | |a Azuaje, Francisco |e verfasserin |4 aut | |
| 700 | 1 | |a Skupin, Alexander |e verfasserin |4 aut | |
| 700 | 1 | |a Balling, Rudi |e verfasserin |4 aut | |
| 700 | 1 | |a Biber, Knut |e verfasserin |0 (orcid)0000-0002-8815-1705 |4 aut | |
| 700 | 1 | |a Niclou, Simone P |e verfasserin |0 (orcid)0000-0002-3417-9534 |4 aut | |
| 700 | 1 | |a Michelucci, Alessandro |e verfasserin |0 (orcid)0000-0003-1230-061X |4 aut | |
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10.15252/embr.201846171 doi (DE-627)SPR058085351 (SPR)embr.201846171-e DE-627 ger DE-627 rakwb eng Sousa, Carole verfasserin aut Single‐cell transcriptomics reveals distinct inflammation‐induced microglia signatures 2018 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s) 2018 Abstract Microglia are specialized parenchymal‐resident phagocytes of the central nervous system (CNS) that actively support, defend and modulate the neural environment. Dysfunctional microglial responses are thought to worsen CNS diseases; nevertheless, their impact during neuroinflammatory processes remains largely obscure. Here, using a combination of single‐cell RNA sequencing and multicolour flow cytometry, we comprehensively profile microglia in the brain of lipopolysaccharide (LPS)‐injected mice. By excluding the contribution of other immune CNS‐resident and peripheral cells, we show that microglia isolated from LPS‐injected mice display a global downregulation of their homeostatic signature together with an upregulation of inflammatory genes. Notably, we identify distinct microglial activated profiles under inflammatory conditions, which greatly differ from neurodegenerative disease‐associated profiles. These results provide insights into microglial heterogeneity and establish a resource for the identification of specific phenotypes in CNS disorders, such as neuroinflammatory and neurodegenerative diseases. Synopsis Using single‐cell transcriptomics and multicolour flow cytometry this study presents comprehensive profiles of microglia in LPS‐injected mice, providing insight into microglia heterogeneity, and establishing a resource for the identification of specific phenotypes in CNS disorders. Microglia homeostatic signatures are mainly lost upon acute systemic inflammation.Inflammation‐induced microglia segregate into two distinct reactive states.Inflammation‐induced microglia signatures are distinct from neurodegenerative disease‐associated profiles. Graphical Abstract Using single‐cell transcriptomics and multicolour flow cytometry this study presents comprehensive profiles of microglia in LPS‐injected mice, providing insight into microglia heterogeneity, and establishing a resource for the identification of specific phenotypes in CNS disorders. heterogeneity (dpeaa)DE-He213 lipopolysaccharide (dpeaa)DE-He213 microglia (dpeaa)DE-He213 neuroinflammation (dpeaa)DE-He213 single‐cell RNA‐seq (dpeaa)DE-He213 Golebiewska, Anna verfasserin aut Poovathingal, Suresh K verfasserin aut Kaoma, Tony verfasserin aut Pires‐Afonso, Yolanda verfasserin aut Martina, Silvia verfasserin aut Coowar, Djalil verfasserin aut Azuaje, Francisco verfasserin aut Skupin, Alexander verfasserin aut Balling, Rudi verfasserin aut Biber, Knut verfasserin (orcid)0000-0002-8815-1705 aut Niclou, Simone P verfasserin (orcid)0000-0002-3417-9534 aut Michelucci, Alessandro verfasserin (orcid)0000-0003-1230-061X aut Enthalten in EMBO Reports Nature Publishing Group UK, 2023 19(2018), 11 vom: 11. Sept. (DE-627)320645622 (DE-600)2025376-X 1469-3178 nnns volume:19 year:2018 number:11 day:11 month:09 https://dx.doi.org/10.15252/embr.201846171 X:SPRINGER Resolving-System kostenfrei Volltext SYSFLAG_0 GBV_SPRINGER GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_72 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_161 GBV_ILN_168 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_211 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_252 GBV_ILN_266 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2018 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4029 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4116 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4155 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4311 GBV_ILN_4313 GBV_ILN_4314 GBV_ILN_4318 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4393 GBV_ILN_4598 GBV_ILN_4700 AR 19 2018 11 11 09 |
| spelling |
10.15252/embr.201846171 doi (DE-627)SPR058085351 (SPR)embr.201846171-e DE-627 ger DE-627 rakwb eng Sousa, Carole verfasserin aut Single‐cell transcriptomics reveals distinct inflammation‐induced microglia signatures 2018 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s) 2018 Abstract Microglia are specialized parenchymal‐resident phagocytes of the central nervous system (CNS) that actively support, defend and modulate the neural environment. Dysfunctional microglial responses are thought to worsen CNS diseases; nevertheless, their impact during neuroinflammatory processes remains largely obscure. Here, using a combination of single‐cell RNA sequencing and multicolour flow cytometry, we comprehensively profile microglia in the brain of lipopolysaccharide (LPS)‐injected mice. By excluding the contribution of other immune CNS‐resident and peripheral cells, we show that microglia isolated from LPS‐injected mice display a global downregulation of their homeostatic signature together with an upregulation of inflammatory genes. Notably, we identify distinct microglial activated profiles under inflammatory conditions, which greatly differ from neurodegenerative disease‐associated profiles. These results provide insights into microglial heterogeneity and establish a resource for the identification of specific phenotypes in CNS disorders, such as neuroinflammatory and neurodegenerative diseases. Synopsis Using single‐cell transcriptomics and multicolour flow cytometry this study presents comprehensive profiles of microglia in LPS‐injected mice, providing insight into microglia heterogeneity, and establishing a resource for the identification of specific phenotypes in CNS disorders. Microglia homeostatic signatures are mainly lost upon acute systemic inflammation.Inflammation‐induced microglia segregate into two distinct reactive states.Inflammation‐induced microglia signatures are distinct from neurodegenerative disease‐associated profiles. Graphical Abstract Using single‐cell transcriptomics and multicolour flow cytometry this study presents comprehensive profiles of microglia in LPS‐injected mice, providing insight into microglia heterogeneity, and establishing a resource for the identification of specific phenotypes in CNS disorders. heterogeneity (dpeaa)DE-He213 lipopolysaccharide (dpeaa)DE-He213 microglia (dpeaa)DE-He213 neuroinflammation (dpeaa)DE-He213 single‐cell RNA‐seq (dpeaa)DE-He213 Golebiewska, Anna verfasserin aut Poovathingal, Suresh K verfasserin aut Kaoma, Tony verfasserin aut Pires‐Afonso, Yolanda verfasserin aut Martina, Silvia verfasserin aut Coowar, Djalil verfasserin aut Azuaje, Francisco verfasserin aut Skupin, Alexander verfasserin aut Balling, Rudi verfasserin aut Biber, Knut verfasserin (orcid)0000-0002-8815-1705 aut Niclou, Simone P verfasserin (orcid)0000-0002-3417-9534 aut Michelucci, Alessandro verfasserin (orcid)0000-0003-1230-061X aut Enthalten in EMBO Reports Nature Publishing Group UK, 2023 19(2018), 11 vom: 11. Sept. (DE-627)320645622 (DE-600)2025376-X 1469-3178 nnns volume:19 year:2018 number:11 day:11 month:09 https://dx.doi.org/10.15252/embr.201846171 X:SPRINGER Resolving-System kostenfrei Volltext SYSFLAG_0 GBV_SPRINGER GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_72 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_161 GBV_ILN_168 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_211 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_252 GBV_ILN_266 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2018 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4029 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4116 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4155 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4311 GBV_ILN_4313 GBV_ILN_4314 GBV_ILN_4318 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4393 GBV_ILN_4598 GBV_ILN_4700 AR 19 2018 11 11 09 |
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10.15252/embr.201846171 doi (DE-627)SPR058085351 (SPR)embr.201846171-e DE-627 ger DE-627 rakwb eng Sousa, Carole verfasserin aut Single‐cell transcriptomics reveals distinct inflammation‐induced microglia signatures 2018 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s) 2018 Abstract Microglia are specialized parenchymal‐resident phagocytes of the central nervous system (CNS) that actively support, defend and modulate the neural environment. Dysfunctional microglial responses are thought to worsen CNS diseases; nevertheless, their impact during neuroinflammatory processes remains largely obscure. Here, using a combination of single‐cell RNA sequencing and multicolour flow cytometry, we comprehensively profile microglia in the brain of lipopolysaccharide (LPS)‐injected mice. By excluding the contribution of other immune CNS‐resident and peripheral cells, we show that microglia isolated from LPS‐injected mice display a global downregulation of their homeostatic signature together with an upregulation of inflammatory genes. Notably, we identify distinct microglial activated profiles under inflammatory conditions, which greatly differ from neurodegenerative disease‐associated profiles. These results provide insights into microglial heterogeneity and establish a resource for the identification of specific phenotypes in CNS disorders, such as neuroinflammatory and neurodegenerative diseases. Synopsis Using single‐cell transcriptomics and multicolour flow cytometry this study presents comprehensive profiles of microglia in LPS‐injected mice, providing insight into microglia heterogeneity, and establishing a resource for the identification of specific phenotypes in CNS disorders. Microglia homeostatic signatures are mainly lost upon acute systemic inflammation.Inflammation‐induced microglia segregate into two distinct reactive states.Inflammation‐induced microglia signatures are distinct from neurodegenerative disease‐associated profiles. Graphical Abstract Using single‐cell transcriptomics and multicolour flow cytometry this study presents comprehensive profiles of microglia in LPS‐injected mice, providing insight into microglia heterogeneity, and establishing a resource for the identification of specific phenotypes in CNS disorders. heterogeneity (dpeaa)DE-He213 lipopolysaccharide (dpeaa)DE-He213 microglia (dpeaa)DE-He213 neuroinflammation (dpeaa)DE-He213 single‐cell RNA‐seq (dpeaa)DE-He213 Golebiewska, Anna verfasserin aut Poovathingal, Suresh K verfasserin aut Kaoma, Tony verfasserin aut Pires‐Afonso, Yolanda verfasserin aut Martina, Silvia verfasserin aut Coowar, Djalil verfasserin aut Azuaje, Francisco verfasserin aut Skupin, Alexander verfasserin aut Balling, Rudi verfasserin aut Biber, Knut verfasserin (orcid)0000-0002-8815-1705 aut Niclou, Simone P verfasserin (orcid)0000-0002-3417-9534 aut Michelucci, Alessandro verfasserin (orcid)0000-0003-1230-061X aut Enthalten in EMBO Reports Nature Publishing Group UK, 2023 19(2018), 11 vom: 11. Sept. (DE-627)320645622 (DE-600)2025376-X 1469-3178 nnns volume:19 year:2018 number:11 day:11 month:09 https://dx.doi.org/10.15252/embr.201846171 X:SPRINGER Resolving-System kostenfrei Volltext SYSFLAG_0 GBV_SPRINGER GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_72 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_161 GBV_ILN_168 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_211 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_252 GBV_ILN_266 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2018 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4029 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4116 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4155 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4311 GBV_ILN_4313 GBV_ILN_4314 GBV_ILN_4318 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4393 GBV_ILN_4598 GBV_ILN_4700 AR 19 2018 11 11 09 |
| allfieldsGer |
10.15252/embr.201846171 doi (DE-627)SPR058085351 (SPR)embr.201846171-e DE-627 ger DE-627 rakwb eng Sousa, Carole verfasserin aut Single‐cell transcriptomics reveals distinct inflammation‐induced microglia signatures 2018 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s) 2018 Abstract Microglia are specialized parenchymal‐resident phagocytes of the central nervous system (CNS) that actively support, defend and modulate the neural environment. Dysfunctional microglial responses are thought to worsen CNS diseases; nevertheless, their impact during neuroinflammatory processes remains largely obscure. Here, using a combination of single‐cell RNA sequencing and multicolour flow cytometry, we comprehensively profile microglia in the brain of lipopolysaccharide (LPS)‐injected mice. By excluding the contribution of other immune CNS‐resident and peripheral cells, we show that microglia isolated from LPS‐injected mice display a global downregulation of their homeostatic signature together with an upregulation of inflammatory genes. Notably, we identify distinct microglial activated profiles under inflammatory conditions, which greatly differ from neurodegenerative disease‐associated profiles. These results provide insights into microglial heterogeneity and establish a resource for the identification of specific phenotypes in CNS disorders, such as neuroinflammatory and neurodegenerative diseases. Synopsis Using single‐cell transcriptomics and multicolour flow cytometry this study presents comprehensive profiles of microglia in LPS‐injected mice, providing insight into microglia heterogeneity, and establishing a resource for the identification of specific phenotypes in CNS disorders. Microglia homeostatic signatures are mainly lost upon acute systemic inflammation.Inflammation‐induced microglia segregate into two distinct reactive states.Inflammation‐induced microglia signatures are distinct from neurodegenerative disease‐associated profiles. Graphical Abstract Using single‐cell transcriptomics and multicolour flow cytometry this study presents comprehensive profiles of microglia in LPS‐injected mice, providing insight into microglia heterogeneity, and establishing a resource for the identification of specific phenotypes in CNS disorders. heterogeneity (dpeaa)DE-He213 lipopolysaccharide (dpeaa)DE-He213 microglia (dpeaa)DE-He213 neuroinflammation (dpeaa)DE-He213 single‐cell RNA‐seq (dpeaa)DE-He213 Golebiewska, Anna verfasserin aut Poovathingal, Suresh K verfasserin aut Kaoma, Tony verfasserin aut Pires‐Afonso, Yolanda verfasserin aut Martina, Silvia verfasserin aut Coowar, Djalil verfasserin aut Azuaje, Francisco verfasserin aut Skupin, Alexander verfasserin aut Balling, Rudi verfasserin aut Biber, Knut verfasserin (orcid)0000-0002-8815-1705 aut Niclou, Simone P verfasserin (orcid)0000-0002-3417-9534 aut Michelucci, Alessandro verfasserin (orcid)0000-0003-1230-061X aut Enthalten in EMBO Reports Nature Publishing Group UK, 2023 19(2018), 11 vom: 11. Sept. (DE-627)320645622 (DE-600)2025376-X 1469-3178 nnns volume:19 year:2018 number:11 day:11 month:09 https://dx.doi.org/10.15252/embr.201846171 X:SPRINGER Resolving-System kostenfrei Volltext SYSFLAG_0 GBV_SPRINGER GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_72 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_161 GBV_ILN_168 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_211 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_252 GBV_ILN_266 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2018 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4029 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4116 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4155 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4311 GBV_ILN_4313 GBV_ILN_4314 GBV_ILN_4318 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4393 GBV_ILN_4598 GBV_ILN_4700 AR 19 2018 11 11 09 |
| allfieldsSound |
10.15252/embr.201846171 doi (DE-627)SPR058085351 (SPR)embr.201846171-e DE-627 ger DE-627 rakwb eng Sousa, Carole verfasserin aut Single‐cell transcriptomics reveals distinct inflammation‐induced microglia signatures 2018 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s) 2018 Abstract Microglia are specialized parenchymal‐resident phagocytes of the central nervous system (CNS) that actively support, defend and modulate the neural environment. Dysfunctional microglial responses are thought to worsen CNS diseases; nevertheless, their impact during neuroinflammatory processes remains largely obscure. Here, using a combination of single‐cell RNA sequencing and multicolour flow cytometry, we comprehensively profile microglia in the brain of lipopolysaccharide (LPS)‐injected mice. By excluding the contribution of other immune CNS‐resident and peripheral cells, we show that microglia isolated from LPS‐injected mice display a global downregulation of their homeostatic signature together with an upregulation of inflammatory genes. Notably, we identify distinct microglial activated profiles under inflammatory conditions, which greatly differ from neurodegenerative disease‐associated profiles. These results provide insights into microglial heterogeneity and establish a resource for the identification of specific phenotypes in CNS disorders, such as neuroinflammatory and neurodegenerative diseases. Synopsis Using single‐cell transcriptomics and multicolour flow cytometry this study presents comprehensive profiles of microglia in LPS‐injected mice, providing insight into microglia heterogeneity, and establishing a resource for the identification of specific phenotypes in CNS disorders. Microglia homeostatic signatures are mainly lost upon acute systemic inflammation.Inflammation‐induced microglia segregate into two distinct reactive states.Inflammation‐induced microglia signatures are distinct from neurodegenerative disease‐associated profiles. Graphical Abstract Using single‐cell transcriptomics and multicolour flow cytometry this study presents comprehensive profiles of microglia in LPS‐injected mice, providing insight into microglia heterogeneity, and establishing a resource for the identification of specific phenotypes in CNS disorders. heterogeneity (dpeaa)DE-He213 lipopolysaccharide (dpeaa)DE-He213 microglia (dpeaa)DE-He213 neuroinflammation (dpeaa)DE-He213 single‐cell RNA‐seq (dpeaa)DE-He213 Golebiewska, Anna verfasserin aut Poovathingal, Suresh K verfasserin aut Kaoma, Tony verfasserin aut Pires‐Afonso, Yolanda verfasserin aut Martina, Silvia verfasserin aut Coowar, Djalil verfasserin aut Azuaje, Francisco verfasserin aut Skupin, Alexander verfasserin aut Balling, Rudi verfasserin aut Biber, Knut verfasserin (orcid)0000-0002-8815-1705 aut Niclou, Simone P verfasserin (orcid)0000-0002-3417-9534 aut Michelucci, Alessandro verfasserin (orcid)0000-0003-1230-061X aut Enthalten in EMBO Reports Nature Publishing Group UK, 2023 19(2018), 11 vom: 11. Sept. (DE-627)320645622 (DE-600)2025376-X 1469-3178 nnns volume:19 year:2018 number:11 day:11 month:09 https://dx.doi.org/10.15252/embr.201846171 X:SPRINGER Resolving-System kostenfrei Volltext SYSFLAG_0 GBV_SPRINGER GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_72 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_138 GBV_ILN_150 GBV_ILN_151 GBV_ILN_161 GBV_ILN_168 GBV_ILN_170 GBV_ILN_171 GBV_ILN_187 GBV_ILN_211 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_252 GBV_ILN_266 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_636 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2018 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2119 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2188 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2470 GBV_ILN_2472 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4029 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4116 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4155 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4311 GBV_ILN_4313 GBV_ILN_4314 GBV_ILN_4318 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4393 GBV_ILN_4598 GBV_ILN_4700 AR 19 2018 11 11 09 |
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Enthalten in EMBO Reports 19(2018), 11 vom: 11. Sept. volume:19 year:2018 number:11 day:11 month:09 |
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Enthalten in EMBO Reports 19(2018), 11 vom: 11. Sept. volume:19 year:2018 number:11 day:11 month:09 |
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Sousa, Carole @@aut@@ Golebiewska, Anna @@aut@@ Poovathingal, Suresh K @@aut@@ Kaoma, Tony @@aut@@ Pires‐Afonso, Yolanda @@aut@@ Martina, Silvia @@aut@@ Coowar, Djalil @@aut@@ Azuaje, Francisco @@aut@@ Skupin, Alexander @@aut@@ Balling, Rudi @@aut@@ Biber, Knut @@aut@@ Niclou, Simone P @@aut@@ Michelucci, Alessandro @@aut@@ |
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2018-09-11T00:00:00Z |
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Dysfunctional microglial responses are thought to worsen CNS diseases; nevertheless, their impact during neuroinflammatory processes remains largely obscure. Here, using a combination of single‐cell RNA sequencing and multicolour flow cytometry, we comprehensively profile microglia in the brain of lipopolysaccharide (LPS)‐injected mice. By excluding the contribution of other immune CNS‐resident and peripheral cells, we show that microglia isolated from LPS‐injected mice display a global downregulation of their homeostatic signature together with an upregulation of inflammatory genes. Notably, we identify distinct microglial activated profiles under inflammatory conditions, which greatly differ from neurodegenerative disease‐associated profiles. These results provide insights into microglial heterogeneity and establish a resource for the identification of specific phenotypes in CNS disorders, such as neuroinflammatory and neurodegenerative diseases.</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a">Synopsis Using single‐cell transcriptomics and multicolour flow cytometry this study presents comprehensive profiles of microglia in LPS‐injected mice, providing insight into microglia heterogeneity, and establishing a resource for the identification of specific phenotypes in CNS disorders. Microglia homeostatic signatures are mainly lost upon acute systemic inflammation.Inflammation‐induced microglia segregate into two distinct reactive states.Inflammation‐induced microglia signatures are distinct from neurodegenerative disease‐associated profiles.</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a">Graphical Abstract Using single‐cell transcriptomics and multicolour flow cytometry this study presents comprehensive profiles of microglia in LPS‐injected mice, providing insight into microglia heterogeneity, and establishing a resource for the identification of specific phenotypes in CNS disorders.</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">heterogeneity</subfield><subfield code="7">(dpeaa)DE-He213</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">lipopolysaccharide</subfield><subfield code="7">(dpeaa)DE-He213</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">microglia</subfield><subfield code="7">(dpeaa)DE-He213</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">neuroinflammation</subfield><subfield code="7">(dpeaa)DE-He213</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">single‐cell RNA‐seq</subfield><subfield code="7">(dpeaa)DE-He213</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Golebiewska, Anna</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Poovathingal, Suresh K</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Kaoma, Tony</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Pires‐Afonso, Yolanda</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Martina, Silvia</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Coowar, Djalil</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Azuaje, Francisco</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Skupin, Alexander</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Balling, Rudi</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Biber, Knut</subfield><subfield code="e">verfasserin</subfield><subfield code="0">(orcid)0000-0002-8815-1705</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Niclou, Simone P</subfield><subfield code="e">verfasserin</subfield><subfield code="0">(orcid)0000-0002-3417-9534</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Michelucci, Alessandro</subfield><subfield code="e">verfasserin</subfield><subfield code="0">(orcid)0000-0003-1230-061X</subfield><subfield code="4">aut</subfield></datafield><datafield tag="773" ind1="0" ind2="8"><subfield code="i">Enthalten in</subfield><subfield code="t">EMBO Reports</subfield><subfield code="d">Nature Publishing Group UK, 2023</subfield><subfield code="g">19(2018), 11 vom: 11. 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Sousa, Carole |
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Sousa, Carole misc heterogeneity misc lipopolysaccharide misc microglia misc neuroinflammation misc single‐cell RNA‐seq Single‐cell transcriptomics reveals distinct inflammation‐induced microglia signatures |
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Single‐cell transcriptomics reveals distinct inflammation‐induced microglia signatures heterogeneity (dpeaa)DE-He213 lipopolysaccharide (dpeaa)DE-He213 microglia (dpeaa)DE-He213 neuroinflammation (dpeaa)DE-He213 single‐cell RNA‐seq (dpeaa)DE-He213 |
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misc heterogeneity misc lipopolysaccharide misc microglia misc neuroinflammation misc single‐cell RNA‐seq |
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Single‐cell transcriptomics reveals distinct inflammation‐induced microglia signatures |
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Single‐cell transcriptomics reveals distinct inflammation‐induced microglia signatures |
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Sousa, Carole |
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EMBO Reports |
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EMBO Reports |
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eng |
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2018 |
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Sousa, Carole Golebiewska, Anna Poovathingal, Suresh K Kaoma, Tony Pires‐Afonso, Yolanda Martina, Silvia Coowar, Djalil Azuaje, Francisco Skupin, Alexander Balling, Rudi Biber, Knut Niclou, Simone P Michelucci, Alessandro |
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19 |
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Elektronische Aufsätze |
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Sousa, Carole |
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10.15252/embr.201846171 |
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(ORCID)0000-0002-8815-1705 (ORCID)0000-0002-3417-9534 (ORCID)0000-0003-1230-061X |
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(orcid)0000-0002-8815-1705 (orcid)0000-0002-3417-9534 (orcid)0000-0003-1230-061X |
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verfasserin |
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single‐cell transcriptomics reveals distinct inflammation‐induced microglia signatures |
| title_auth |
Single‐cell transcriptomics reveals distinct inflammation‐induced microglia signatures |
| abstract |
Abstract Microglia are specialized parenchymal‐resident phagocytes of the central nervous system (CNS) that actively support, defend and modulate the neural environment. Dysfunctional microglial responses are thought to worsen CNS diseases; nevertheless, their impact during neuroinflammatory processes remains largely obscure. Here, using a combination of single‐cell RNA sequencing and multicolour flow cytometry, we comprehensively profile microglia in the brain of lipopolysaccharide (LPS)‐injected mice. By excluding the contribution of other immune CNS‐resident and peripheral cells, we show that microglia isolated from LPS‐injected mice display a global downregulation of their homeostatic signature together with an upregulation of inflammatory genes. Notably, we identify distinct microglial activated profiles under inflammatory conditions, which greatly differ from neurodegenerative disease‐associated profiles. These results provide insights into microglial heterogeneity and establish a resource for the identification of specific phenotypes in CNS disorders, such as neuroinflammatory and neurodegenerative diseases. Synopsis Using single‐cell transcriptomics and multicolour flow cytometry this study presents comprehensive profiles of microglia in LPS‐injected mice, providing insight into microglia heterogeneity, and establishing a resource for the identification of specific phenotypes in CNS disorders. Microglia homeostatic signatures are mainly lost upon acute systemic inflammation.Inflammation‐induced microglia segregate into two distinct reactive states.Inflammation‐induced microglia signatures are distinct from neurodegenerative disease‐associated profiles. Graphical Abstract Using single‐cell transcriptomics and multicolour flow cytometry this study presents comprehensive profiles of microglia in LPS‐injected mice, providing insight into microglia heterogeneity, and establishing a resource for the identification of specific phenotypes in CNS disorders. © The Author(s) 2018 |
| abstractGer |
Abstract Microglia are specialized parenchymal‐resident phagocytes of the central nervous system (CNS) that actively support, defend and modulate the neural environment. Dysfunctional microglial responses are thought to worsen CNS diseases; nevertheless, their impact during neuroinflammatory processes remains largely obscure. Here, using a combination of single‐cell RNA sequencing and multicolour flow cytometry, we comprehensively profile microglia in the brain of lipopolysaccharide (LPS)‐injected mice. By excluding the contribution of other immune CNS‐resident and peripheral cells, we show that microglia isolated from LPS‐injected mice display a global downregulation of their homeostatic signature together with an upregulation of inflammatory genes. Notably, we identify distinct microglial activated profiles under inflammatory conditions, which greatly differ from neurodegenerative disease‐associated profiles. These results provide insights into microglial heterogeneity and establish a resource for the identification of specific phenotypes in CNS disorders, such as neuroinflammatory and neurodegenerative diseases. Synopsis Using single‐cell transcriptomics and multicolour flow cytometry this study presents comprehensive profiles of microglia in LPS‐injected mice, providing insight into microglia heterogeneity, and establishing a resource for the identification of specific phenotypes in CNS disorders. Microglia homeostatic signatures are mainly lost upon acute systemic inflammation.Inflammation‐induced microglia segregate into two distinct reactive states.Inflammation‐induced microglia signatures are distinct from neurodegenerative disease‐associated profiles. Graphical Abstract Using single‐cell transcriptomics and multicolour flow cytometry this study presents comprehensive profiles of microglia in LPS‐injected mice, providing insight into microglia heterogeneity, and establishing a resource for the identification of specific phenotypes in CNS disorders. © The Author(s) 2018 |
| abstract_unstemmed |
Abstract Microglia are specialized parenchymal‐resident phagocytes of the central nervous system (CNS) that actively support, defend and modulate the neural environment. Dysfunctional microglial responses are thought to worsen CNS diseases; nevertheless, their impact during neuroinflammatory processes remains largely obscure. Here, using a combination of single‐cell RNA sequencing and multicolour flow cytometry, we comprehensively profile microglia in the brain of lipopolysaccharide (LPS)‐injected mice. By excluding the contribution of other immune CNS‐resident and peripheral cells, we show that microglia isolated from LPS‐injected mice display a global downregulation of their homeostatic signature together with an upregulation of inflammatory genes. Notably, we identify distinct microglial activated profiles under inflammatory conditions, which greatly differ from neurodegenerative disease‐associated profiles. These results provide insights into microglial heterogeneity and establish a resource for the identification of specific phenotypes in CNS disorders, such as neuroinflammatory and neurodegenerative diseases. Synopsis Using single‐cell transcriptomics and multicolour flow cytometry this study presents comprehensive profiles of microglia in LPS‐injected mice, providing insight into microglia heterogeneity, and establishing a resource for the identification of specific phenotypes in CNS disorders. Microglia homeostatic signatures are mainly lost upon acute systemic inflammation.Inflammation‐induced microglia segregate into two distinct reactive states.Inflammation‐induced microglia signatures are distinct from neurodegenerative disease‐associated profiles. Graphical Abstract Using single‐cell transcriptomics and multicolour flow cytometry this study presents comprehensive profiles of microglia in LPS‐injected mice, providing insight into microglia heterogeneity, and establishing a resource for the identification of specific phenotypes in CNS disorders. © The Author(s) 2018 |
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Single‐cell transcriptomics reveals distinct inflammation‐induced microglia signatures |
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Golebiewska, Anna Poovathingal, Suresh K Kaoma, Tony Pires‐Afonso, Yolanda Martina, Silvia Coowar, Djalil Azuaje, Francisco Skupin, Alexander Balling, Rudi Biber, Knut Niclou, Simone P Michelucci, Alessandro |
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| score |
7.400757 |