Co‐recruitment analysis of the CBL and CBLB signalosomes in primary T cells identifies CD5 as a key regulator of TCR‐induced ubiquitylation
Abstract T‐cell receptor (TCR) signaling is essential for the function of T cells and negatively regulated by the E3 ubiquitin–protein ligases CBL and CBLB. Here, we combined mouse genetics and affinity purification coupled to quantitative mass spectrometry to monitor the dynamics of the CBL and CBL...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
Voisinne, Guillaume [verfasserIn] García‐Blesa, Antonio [verfasserIn] Chaoui, Karima [verfasserIn] Fiore, Frédéric [verfasserIn] Bergot, Elise [verfasserIn] Girard, Laura [verfasserIn] Malissen, Marie [verfasserIn] Burlet‐Schiltz, Odile [verfasserIn] Gonzalez de Peredo, Anne [verfasserIn] Malissen, Bernard [verfasserIn] Roncagalli, Romain [verfasserIn] |
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| Format: |
E-Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
2016 |
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| Schlagwörter: |
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| Anmerkung: |
© The Author(s) 2016 |
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| Übergeordnetes Werk: |
Enthalten in: Molecular Systems Biology - Nature Publishing Group UK, 2023, 12(2016), 7 vom: 29. Juli |
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| Übergeordnetes Werk: |
volume:12 ; year:2016 ; number:7 ; day:29 ; month:07 |
| Links: |
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| DOI / URN: |
10.15252/msb.20166837 |
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| Katalog-ID: |
SPR058092366 |
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| 100 | 1 | |a Voisinne, Guillaume |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Co‐recruitment analysis of the CBL and CBLB signalosomes in primary T cells identifies CD5 as a key regulator of TCR‐induced ubiquitylation |
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| 520 | |a Abstract T‐cell receptor (TCR) signaling is essential for the function of T cells and negatively regulated by the E3 ubiquitin–protein ligases CBL and CBLB. Here, we combined mouse genetics and affinity purification coupled to quantitative mass spectrometry to monitor the dynamics of the CBL and CBLB signaling complexes that assemble in normal T cells over 600 seconds of TCR stimulation. We identify most previously known CBL and CBLB interacting partners, as well as a majority of proteins that have not yet been implicated in those signaling complexes. We exploit correlations in protein association with CBL and CBLB as a function of time of TCR stimulation for predicting the occurrence of direct physical association between them. By combining co‐recruitment analysis with biochemical analysis, we demonstrated that the CD5 transmembrane receptor constitutes a key scaffold for CBL‐ and CBLB‐mediated ubiquitylation following TCR engagement. Our results offer an integrated view of the CBL and CBLB signaling complexes induced by TCR stimulation and provide a molecular basis for their negative regulatory function in normal T cells. | ||
| 520 | |a Synopsis The composition and dynamics of the signalosomes operated by the E3 ubiquitin–protein ligases CBL and CBLB were determined in primary T cells after TCR stimulation. Analysis of correlations in protein association as a function of time reveals the importance of the CD5 transmembrane receptor in the regulation of ubiquitylation. The signalosomes of the E3‐ubiquitin ligases CBL and CBLB exhibit both redundant and distinct features in mature $ CD4^{+} $ T cells.Analysis of correlations in protein association with CBL and CBLB accurately predicts interactions between recruited proteins.CD5 is identified as a key regulator of CBL‐ and CBLB‐mediated ubiquitylation following TCR engagement. | ||
| 520 | |a Graphical Abstract The composition and dynamics of the signalosomes operated by the E3 ubiquitin–protein ligases CBL and CBLB were determined in primary T cells after TCR stimulation. Analysis of correlations in protein association as a function of time reveals the importance of the CD5 transmembrane receptor in the regulation of ubiquitylation. | ||
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| 700 | 1 | |a García‐Blesa, Antonio |e verfasserin |4 aut | |
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| 700 | 1 | |a Bergot, Elise |e verfasserin |4 aut | |
| 700 | 1 | |a Girard, Laura |e verfasserin |4 aut | |
| 700 | 1 | |a Malissen, Marie |e verfasserin |4 aut | |
| 700 | 1 | |a Burlet‐Schiltz, Odile |e verfasserin |4 aut | |
| 700 | 1 | |a Gonzalez de Peredo, Anne |e verfasserin |4 aut | |
| 700 | 1 | |a Malissen, Bernard |e verfasserin |0 (orcid)0000-0003-1340-9342 |4 aut | |
| 700 | 1 | |a Roncagalli, Romain |e verfasserin |0 (orcid)0000-0001-7554-0552 |4 aut | |
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10.15252/msb.20166837 doi (DE-627)SPR058092366 (SPR)msb.20166837-e DE-627 ger DE-627 rakwb eng Voisinne, Guillaume verfasserin aut Co‐recruitment analysis of the CBL and CBLB signalosomes in primary T cells identifies CD5 as a key regulator of TCR‐induced ubiquitylation 2016 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s) 2016 Abstract T‐cell receptor (TCR) signaling is essential for the function of T cells and negatively regulated by the E3 ubiquitin–protein ligases CBL and CBLB. Here, we combined mouse genetics and affinity purification coupled to quantitative mass spectrometry to monitor the dynamics of the CBL and CBLB signaling complexes that assemble in normal T cells over 600 seconds of TCR stimulation. We identify most previously known CBL and CBLB interacting partners, as well as a majority of proteins that have not yet been implicated in those signaling complexes. We exploit correlations in protein association with CBL and CBLB as a function of time of TCR stimulation for predicting the occurrence of direct physical association between them. By combining co‐recruitment analysis with biochemical analysis, we demonstrated that the CD5 transmembrane receptor constitutes a key scaffold for CBL‐ and CBLB‐mediated ubiquitylation following TCR engagement. Our results offer an integrated view of the CBL and CBLB signaling complexes induced by TCR stimulation and provide a molecular basis for their negative regulatory function in normal T cells. Synopsis The composition and dynamics of the signalosomes operated by the E3 ubiquitin–protein ligases CBL and CBLB were determined in primary T cells after TCR stimulation. Analysis of correlations in protein association as a function of time reveals the importance of the CD5 transmembrane receptor in the regulation of ubiquitylation. The signalosomes of the E3‐ubiquitin ligases CBL and CBLB exhibit both redundant and distinct features in mature $ CD4^{+} $ T cells.Analysis of correlations in protein association with CBL and CBLB accurately predicts interactions between recruited proteins.CD5 is identified as a key regulator of CBL‐ and CBLB‐mediated ubiquitylation following TCR engagement. Graphical Abstract The composition and dynamics of the signalosomes operated by the E3 ubiquitin–protein ligases CBL and CBLB were determined in primary T cells after TCR stimulation. Analysis of correlations in protein association as a function of time reveals the importance of the CD5 transmembrane receptor in the regulation of ubiquitylation. CBL (dpeaa)DE-He213 CBLB (dpeaa)DE-He213 CD5 (dpeaa)DE-He213 ubiquitylation (dpeaa)DE-He213 García‐Blesa, Antonio verfasserin aut Chaoui, Karima verfasserin aut Fiore, Frédéric verfasserin aut Bergot, Elise verfasserin aut Girard, Laura verfasserin aut Malissen, Marie verfasserin aut Burlet‐Schiltz, Odile verfasserin aut Gonzalez de Peredo, Anne verfasserin aut Malissen, Bernard verfasserin (orcid)0000-0003-1340-9342 aut Roncagalli, Romain verfasserin (orcid)0000-0001-7554-0552 aut Enthalten in Molecular Systems Biology Nature Publishing Group UK, 2023 12(2016), 7 vom: 29. Juli (DE-627)490536905 (DE-600)2193510-5 1744-4292 nnns volume:12 year:2016 number:7 day:29 month:07 https://dx.doi.org/10.15252/msb.20166837 X:SPRINGER Resolving-System kostenfrei Volltext SYSFLAG_0 GBV_SPRINGER GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_72 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_636 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2470 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4029 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4116 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4155 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4311 GBV_ILN_4313 GBV_ILN_4314 GBV_ILN_4315 GBV_ILN_4318 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4393 GBV_ILN_4598 GBV_ILN_4700 AR 12 2016 7 29 07 |
| spelling |
10.15252/msb.20166837 doi (DE-627)SPR058092366 (SPR)msb.20166837-e DE-627 ger DE-627 rakwb eng Voisinne, Guillaume verfasserin aut Co‐recruitment analysis of the CBL and CBLB signalosomes in primary T cells identifies CD5 as a key regulator of TCR‐induced ubiquitylation 2016 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s) 2016 Abstract T‐cell receptor (TCR) signaling is essential for the function of T cells and negatively regulated by the E3 ubiquitin–protein ligases CBL and CBLB. Here, we combined mouse genetics and affinity purification coupled to quantitative mass spectrometry to monitor the dynamics of the CBL and CBLB signaling complexes that assemble in normal T cells over 600 seconds of TCR stimulation. We identify most previously known CBL and CBLB interacting partners, as well as a majority of proteins that have not yet been implicated in those signaling complexes. We exploit correlations in protein association with CBL and CBLB as a function of time of TCR stimulation for predicting the occurrence of direct physical association between them. By combining co‐recruitment analysis with biochemical analysis, we demonstrated that the CD5 transmembrane receptor constitutes a key scaffold for CBL‐ and CBLB‐mediated ubiquitylation following TCR engagement. Our results offer an integrated view of the CBL and CBLB signaling complexes induced by TCR stimulation and provide a molecular basis for their negative regulatory function in normal T cells. Synopsis The composition and dynamics of the signalosomes operated by the E3 ubiquitin–protein ligases CBL and CBLB were determined in primary T cells after TCR stimulation. Analysis of correlations in protein association as a function of time reveals the importance of the CD5 transmembrane receptor in the regulation of ubiquitylation. The signalosomes of the E3‐ubiquitin ligases CBL and CBLB exhibit both redundant and distinct features in mature $ CD4^{+} $ T cells.Analysis of correlations in protein association with CBL and CBLB accurately predicts interactions between recruited proteins.CD5 is identified as a key regulator of CBL‐ and CBLB‐mediated ubiquitylation following TCR engagement. Graphical Abstract The composition and dynamics of the signalosomes operated by the E3 ubiquitin–protein ligases CBL and CBLB were determined in primary T cells after TCR stimulation. Analysis of correlations in protein association as a function of time reveals the importance of the CD5 transmembrane receptor in the regulation of ubiquitylation. CBL (dpeaa)DE-He213 CBLB (dpeaa)DE-He213 CD5 (dpeaa)DE-He213 ubiquitylation (dpeaa)DE-He213 García‐Blesa, Antonio verfasserin aut Chaoui, Karima verfasserin aut Fiore, Frédéric verfasserin aut Bergot, Elise verfasserin aut Girard, Laura verfasserin aut Malissen, Marie verfasserin aut Burlet‐Schiltz, Odile verfasserin aut Gonzalez de Peredo, Anne verfasserin aut Malissen, Bernard verfasserin (orcid)0000-0003-1340-9342 aut Roncagalli, Romain verfasserin (orcid)0000-0001-7554-0552 aut Enthalten in Molecular Systems Biology Nature Publishing Group UK, 2023 12(2016), 7 vom: 29. Juli (DE-627)490536905 (DE-600)2193510-5 1744-4292 nnns volume:12 year:2016 number:7 day:29 month:07 https://dx.doi.org/10.15252/msb.20166837 X:SPRINGER Resolving-System kostenfrei Volltext SYSFLAG_0 GBV_SPRINGER GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_72 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_636 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2470 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4029 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4116 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4155 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4311 GBV_ILN_4313 GBV_ILN_4314 GBV_ILN_4315 GBV_ILN_4318 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4393 GBV_ILN_4598 GBV_ILN_4700 AR 12 2016 7 29 07 |
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10.15252/msb.20166837 doi (DE-627)SPR058092366 (SPR)msb.20166837-e DE-627 ger DE-627 rakwb eng Voisinne, Guillaume verfasserin aut Co‐recruitment analysis of the CBL and CBLB signalosomes in primary T cells identifies CD5 as a key regulator of TCR‐induced ubiquitylation 2016 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s) 2016 Abstract T‐cell receptor (TCR) signaling is essential for the function of T cells and negatively regulated by the E3 ubiquitin–protein ligases CBL and CBLB. Here, we combined mouse genetics and affinity purification coupled to quantitative mass spectrometry to monitor the dynamics of the CBL and CBLB signaling complexes that assemble in normal T cells over 600 seconds of TCR stimulation. We identify most previously known CBL and CBLB interacting partners, as well as a majority of proteins that have not yet been implicated in those signaling complexes. We exploit correlations in protein association with CBL and CBLB as a function of time of TCR stimulation for predicting the occurrence of direct physical association between them. By combining co‐recruitment analysis with biochemical analysis, we demonstrated that the CD5 transmembrane receptor constitutes a key scaffold for CBL‐ and CBLB‐mediated ubiquitylation following TCR engagement. Our results offer an integrated view of the CBL and CBLB signaling complexes induced by TCR stimulation and provide a molecular basis for their negative regulatory function in normal T cells. Synopsis The composition and dynamics of the signalosomes operated by the E3 ubiquitin–protein ligases CBL and CBLB were determined in primary T cells after TCR stimulation. Analysis of correlations in protein association as a function of time reveals the importance of the CD5 transmembrane receptor in the regulation of ubiquitylation. The signalosomes of the E3‐ubiquitin ligases CBL and CBLB exhibit both redundant and distinct features in mature $ CD4^{+} $ T cells.Analysis of correlations in protein association with CBL and CBLB accurately predicts interactions between recruited proteins.CD5 is identified as a key regulator of CBL‐ and CBLB‐mediated ubiquitylation following TCR engagement. Graphical Abstract The composition and dynamics of the signalosomes operated by the E3 ubiquitin–protein ligases CBL and CBLB were determined in primary T cells after TCR stimulation. Analysis of correlations in protein association as a function of time reveals the importance of the CD5 transmembrane receptor in the regulation of ubiquitylation. CBL (dpeaa)DE-He213 CBLB (dpeaa)DE-He213 CD5 (dpeaa)DE-He213 ubiquitylation (dpeaa)DE-He213 García‐Blesa, Antonio verfasserin aut Chaoui, Karima verfasserin aut Fiore, Frédéric verfasserin aut Bergot, Elise verfasserin aut Girard, Laura verfasserin aut Malissen, Marie verfasserin aut Burlet‐Schiltz, Odile verfasserin aut Gonzalez de Peredo, Anne verfasserin aut Malissen, Bernard verfasserin (orcid)0000-0003-1340-9342 aut Roncagalli, Romain verfasserin (orcid)0000-0001-7554-0552 aut Enthalten in Molecular Systems Biology Nature Publishing Group UK, 2023 12(2016), 7 vom: 29. Juli (DE-627)490536905 (DE-600)2193510-5 1744-4292 nnns volume:12 year:2016 number:7 day:29 month:07 https://dx.doi.org/10.15252/msb.20166837 X:SPRINGER Resolving-System kostenfrei Volltext SYSFLAG_0 GBV_SPRINGER GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_72 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_636 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2470 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4029 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4116 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4155 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4311 GBV_ILN_4313 GBV_ILN_4314 GBV_ILN_4315 GBV_ILN_4318 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4393 GBV_ILN_4598 GBV_ILN_4700 AR 12 2016 7 29 07 |
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10.15252/msb.20166837 doi (DE-627)SPR058092366 (SPR)msb.20166837-e DE-627 ger DE-627 rakwb eng Voisinne, Guillaume verfasserin aut Co‐recruitment analysis of the CBL and CBLB signalosomes in primary T cells identifies CD5 as a key regulator of TCR‐induced ubiquitylation 2016 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s) 2016 Abstract T‐cell receptor (TCR) signaling is essential for the function of T cells and negatively regulated by the E3 ubiquitin–protein ligases CBL and CBLB. Here, we combined mouse genetics and affinity purification coupled to quantitative mass spectrometry to monitor the dynamics of the CBL and CBLB signaling complexes that assemble in normal T cells over 600 seconds of TCR stimulation. We identify most previously known CBL and CBLB interacting partners, as well as a majority of proteins that have not yet been implicated in those signaling complexes. We exploit correlations in protein association with CBL and CBLB as a function of time of TCR stimulation for predicting the occurrence of direct physical association between them. By combining co‐recruitment analysis with biochemical analysis, we demonstrated that the CD5 transmembrane receptor constitutes a key scaffold for CBL‐ and CBLB‐mediated ubiquitylation following TCR engagement. Our results offer an integrated view of the CBL and CBLB signaling complexes induced by TCR stimulation and provide a molecular basis for their negative regulatory function in normal T cells. Synopsis The composition and dynamics of the signalosomes operated by the E3 ubiquitin–protein ligases CBL and CBLB were determined in primary T cells after TCR stimulation. Analysis of correlations in protein association as a function of time reveals the importance of the CD5 transmembrane receptor in the regulation of ubiquitylation. The signalosomes of the E3‐ubiquitin ligases CBL and CBLB exhibit both redundant and distinct features in mature $ CD4^{+} $ T cells.Analysis of correlations in protein association with CBL and CBLB accurately predicts interactions between recruited proteins.CD5 is identified as a key regulator of CBL‐ and CBLB‐mediated ubiquitylation following TCR engagement. Graphical Abstract The composition and dynamics of the signalosomes operated by the E3 ubiquitin–protein ligases CBL and CBLB were determined in primary T cells after TCR stimulation. Analysis of correlations in protein association as a function of time reveals the importance of the CD5 transmembrane receptor in the regulation of ubiquitylation. CBL (dpeaa)DE-He213 CBLB (dpeaa)DE-He213 CD5 (dpeaa)DE-He213 ubiquitylation (dpeaa)DE-He213 García‐Blesa, Antonio verfasserin aut Chaoui, Karima verfasserin aut Fiore, Frédéric verfasserin aut Bergot, Elise verfasserin aut Girard, Laura verfasserin aut Malissen, Marie verfasserin aut Burlet‐Schiltz, Odile verfasserin aut Gonzalez de Peredo, Anne verfasserin aut Malissen, Bernard verfasserin (orcid)0000-0003-1340-9342 aut Roncagalli, Romain verfasserin (orcid)0000-0001-7554-0552 aut Enthalten in Molecular Systems Biology Nature Publishing Group UK, 2023 12(2016), 7 vom: 29. Juli (DE-627)490536905 (DE-600)2193510-5 1744-4292 nnns volume:12 year:2016 number:7 day:29 month:07 https://dx.doi.org/10.15252/msb.20166837 X:SPRINGER Resolving-System kostenfrei Volltext SYSFLAG_0 GBV_SPRINGER GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_72 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_636 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2470 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4029 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4116 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4155 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4311 GBV_ILN_4313 GBV_ILN_4314 GBV_ILN_4315 GBV_ILN_4318 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4393 GBV_ILN_4598 GBV_ILN_4700 AR 12 2016 7 29 07 |
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10.15252/msb.20166837 doi (DE-627)SPR058092366 (SPR)msb.20166837-e DE-627 ger DE-627 rakwb eng Voisinne, Guillaume verfasserin aut Co‐recruitment analysis of the CBL and CBLB signalosomes in primary T cells identifies CD5 as a key regulator of TCR‐induced ubiquitylation 2016 Text txt rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier © The Author(s) 2016 Abstract T‐cell receptor (TCR) signaling is essential for the function of T cells and negatively regulated by the E3 ubiquitin–protein ligases CBL and CBLB. Here, we combined mouse genetics and affinity purification coupled to quantitative mass spectrometry to monitor the dynamics of the CBL and CBLB signaling complexes that assemble in normal T cells over 600 seconds of TCR stimulation. We identify most previously known CBL and CBLB interacting partners, as well as a majority of proteins that have not yet been implicated in those signaling complexes. We exploit correlations in protein association with CBL and CBLB as a function of time of TCR stimulation for predicting the occurrence of direct physical association between them. By combining co‐recruitment analysis with biochemical analysis, we demonstrated that the CD5 transmembrane receptor constitutes a key scaffold for CBL‐ and CBLB‐mediated ubiquitylation following TCR engagement. Our results offer an integrated view of the CBL and CBLB signaling complexes induced by TCR stimulation and provide a molecular basis for their negative regulatory function in normal T cells. Synopsis The composition and dynamics of the signalosomes operated by the E3 ubiquitin–protein ligases CBL and CBLB were determined in primary T cells after TCR stimulation. Analysis of correlations in protein association as a function of time reveals the importance of the CD5 transmembrane receptor in the regulation of ubiquitylation. The signalosomes of the E3‐ubiquitin ligases CBL and CBLB exhibit both redundant and distinct features in mature $ CD4^{+} $ T cells.Analysis of correlations in protein association with CBL and CBLB accurately predicts interactions between recruited proteins.CD5 is identified as a key regulator of CBL‐ and CBLB‐mediated ubiquitylation following TCR engagement. Graphical Abstract The composition and dynamics of the signalosomes operated by the E3 ubiquitin–protein ligases CBL and CBLB were determined in primary T cells after TCR stimulation. Analysis of correlations in protein association as a function of time reveals the importance of the CD5 transmembrane receptor in the regulation of ubiquitylation. CBL (dpeaa)DE-He213 CBLB (dpeaa)DE-He213 CD5 (dpeaa)DE-He213 ubiquitylation (dpeaa)DE-He213 García‐Blesa, Antonio verfasserin aut Chaoui, Karima verfasserin aut Fiore, Frédéric verfasserin aut Bergot, Elise verfasserin aut Girard, Laura verfasserin aut Malissen, Marie verfasserin aut Burlet‐Schiltz, Odile verfasserin aut Gonzalez de Peredo, Anne verfasserin aut Malissen, Bernard verfasserin (orcid)0000-0003-1340-9342 aut Roncagalli, Romain verfasserin (orcid)0000-0001-7554-0552 aut Enthalten in Molecular Systems Biology Nature Publishing Group UK, 2023 12(2016), 7 vom: 29. Juli (DE-627)490536905 (DE-600)2193510-5 1744-4292 nnns volume:12 year:2016 number:7 day:29 month:07 https://dx.doi.org/10.15252/msb.20166837 X:SPRINGER Resolving-System kostenfrei Volltext SYSFLAG_0 GBV_SPRINGER GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_72 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_171 GBV_ILN_206 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_636 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2037 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2057 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2093 GBV_ILN_2106 GBV_ILN_2108 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2144 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2470 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_2548 GBV_ILN_4012 GBV_ILN_4029 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4116 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4155 GBV_ILN_4242 GBV_ILN_4246 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4311 GBV_ILN_4313 GBV_ILN_4314 GBV_ILN_4315 GBV_ILN_4318 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4328 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4336 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4393 GBV_ILN_4598 GBV_ILN_4700 AR 12 2016 7 29 07 |
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Enthalten in Molecular Systems Biology 12(2016), 7 vom: 29. Juli volume:12 year:2016 number:7 day:29 month:07 |
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Enthalten in Molecular Systems Biology 12(2016), 7 vom: 29. Juli volume:12 year:2016 number:7 day:29 month:07 |
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Voisinne, Guillaume @@aut@@ García‐Blesa, Antonio @@aut@@ Chaoui, Karima @@aut@@ Fiore, Frédéric @@aut@@ Bergot, Elise @@aut@@ Girard, Laura @@aut@@ Malissen, Marie @@aut@@ Burlet‐Schiltz, Odile @@aut@@ Gonzalez de Peredo, Anne @@aut@@ Malissen, Bernard @@aut@@ Roncagalli, Romain @@aut@@ |
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<?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>01000naa a22002652 4500</leader><controlfield tag="001">SPR058092366</controlfield><controlfield tag="003">DE-627</controlfield><controlfield tag="005">20241025065209.0</controlfield><controlfield tag="007">cr uuu---uuuuu</controlfield><controlfield tag="008">241025s2016 xx |||||o 00| ||eng c</controlfield><datafield tag="024" ind1="7" ind2=" "><subfield code="a">10.15252/msb.20166837</subfield><subfield code="2">doi</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-627)SPR058092366</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(SPR)msb.20166837-e</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-627</subfield><subfield code="b">ger</subfield><subfield code="c">DE-627</subfield><subfield code="e">rakwb</subfield></datafield><datafield tag="041" ind1=" " ind2=" "><subfield code="a">eng</subfield></datafield><datafield tag="100" ind1="1" ind2=" "><subfield code="a">Voisinne, Guillaume</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">Co‐recruitment analysis of the CBL and CBLB signalosomes in primary T cells identifies CD5 as a key regulator of TCR‐induced ubiquitylation</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="c">2016</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="a">Text</subfield><subfield code="b">txt</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="a">Computermedien</subfield><subfield code="b">c</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="a">Online-Ressource</subfield><subfield code="b">cr</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="500" ind1=" " ind2=" "><subfield code="a">© The Author(s) 2016</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a">Abstract T‐cell receptor (TCR) signaling is essential for the function of T cells and negatively regulated by the E3 ubiquitin–protein ligases CBL and CBLB. Here, we combined mouse genetics and affinity purification coupled to quantitative mass spectrometry to monitor the dynamics of the CBL and CBLB signaling complexes that assemble in normal T cells over 600 seconds of TCR stimulation. We identify most previously known CBL and CBLB interacting partners, as well as a majority of proteins that have not yet been implicated in those signaling complexes. We exploit correlations in protein association with CBL and CBLB as a function of time of TCR stimulation for predicting the occurrence of direct physical association between them. By combining co‐recruitment analysis with biochemical analysis, we demonstrated that the CD5 transmembrane receptor constitutes a key scaffold for CBL‐ and CBLB‐mediated ubiquitylation following TCR engagement. Our results offer an integrated view of the CBL and CBLB signaling complexes induced by TCR stimulation and provide a molecular basis for their negative regulatory function in normal T cells.</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a">Synopsis The composition and dynamics of the signalosomes operated by the E3 ubiquitin–protein ligases CBL and CBLB were determined in primary T cells after TCR stimulation. Analysis of correlations in protein association as a function of time reveals the importance of the CD5 transmembrane receptor in the regulation of ubiquitylation. The signalosomes of the E3‐ubiquitin ligases CBL and CBLB exhibit both redundant and distinct features in mature $ CD4^{+} $ T cells.Analysis of correlations in protein association with CBL and CBLB accurately predicts interactions between recruited proteins.CD5 is identified as a key regulator of CBL‐ and CBLB‐mediated ubiquitylation following TCR engagement.</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a">Graphical Abstract The composition and dynamics of the signalosomes operated by the E3 ubiquitin–protein ligases CBL and CBLB were determined in primary T cells after TCR stimulation. 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|
| author |
Voisinne, Guillaume |
| spellingShingle |
Voisinne, Guillaume misc CBL misc CBLB misc CD5 misc ubiquitylation Co‐recruitment analysis of the CBL and CBLB signalosomes in primary T cells identifies CD5 as a key regulator of TCR‐induced ubiquitylation |
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1744-4292 |
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Co‐recruitment analysis of the CBL and CBLB signalosomes in primary T cells identifies CD5 as a key regulator of TCR‐induced ubiquitylation CBL (dpeaa)DE-He213 CBLB (dpeaa)DE-He213 CD5 (dpeaa)DE-He213 ubiquitylation (dpeaa)DE-He213 |
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misc CBL misc CBLB misc CD5 misc ubiquitylation |
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misc CBL misc CBLB misc CD5 misc ubiquitylation |
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misc CBL misc CBLB misc CD5 misc ubiquitylation |
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Elektronische Aufsätze Aufsätze Elektronische Ressource |
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Molecular Systems Biology |
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Co‐recruitment analysis of the CBL and CBLB signalosomes in primary T cells identifies CD5 as a key regulator of TCR‐induced ubiquitylation |
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(DE-627)SPR058092366 (SPR)msb.20166837-e |
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Co‐recruitment analysis of the CBL and CBLB signalosomes in primary T cells identifies CD5 as a key regulator of TCR‐induced ubiquitylation |
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Voisinne, Guillaume |
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Molecular Systems Biology |
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Molecular Systems Biology |
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Voisinne, Guillaume García‐Blesa, Antonio Chaoui, Karima Fiore, Frédéric Bergot, Elise Girard, Laura Malissen, Marie Burlet‐Schiltz, Odile Gonzalez de Peredo, Anne Malissen, Bernard Roncagalli, Romain |
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Elektronische Aufsätze |
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Voisinne, Guillaume |
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10.15252/msb.20166837 |
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co‐recruitment analysis of the cbl and cblb signalosomes in primary t cells identifies cd5 as a key regulator of tcr‐induced ubiquitylation |
| title_auth |
Co‐recruitment analysis of the CBL and CBLB signalosomes in primary T cells identifies CD5 as a key regulator of TCR‐induced ubiquitylation |
| abstract |
Abstract T‐cell receptor (TCR) signaling is essential for the function of T cells and negatively regulated by the E3 ubiquitin–protein ligases CBL and CBLB. Here, we combined mouse genetics and affinity purification coupled to quantitative mass spectrometry to monitor the dynamics of the CBL and CBLB signaling complexes that assemble in normal T cells over 600 seconds of TCR stimulation. We identify most previously known CBL and CBLB interacting partners, as well as a majority of proteins that have not yet been implicated in those signaling complexes. We exploit correlations in protein association with CBL and CBLB as a function of time of TCR stimulation for predicting the occurrence of direct physical association between them. By combining co‐recruitment analysis with biochemical analysis, we demonstrated that the CD5 transmembrane receptor constitutes a key scaffold for CBL‐ and CBLB‐mediated ubiquitylation following TCR engagement. Our results offer an integrated view of the CBL and CBLB signaling complexes induced by TCR stimulation and provide a molecular basis for their negative regulatory function in normal T cells. Synopsis The composition and dynamics of the signalosomes operated by the E3 ubiquitin–protein ligases CBL and CBLB were determined in primary T cells after TCR stimulation. Analysis of correlations in protein association as a function of time reveals the importance of the CD5 transmembrane receptor in the regulation of ubiquitylation. The signalosomes of the E3‐ubiquitin ligases CBL and CBLB exhibit both redundant and distinct features in mature $ CD4^{+} $ T cells.Analysis of correlations in protein association with CBL and CBLB accurately predicts interactions between recruited proteins.CD5 is identified as a key regulator of CBL‐ and CBLB‐mediated ubiquitylation following TCR engagement. Graphical Abstract The composition and dynamics of the signalosomes operated by the E3 ubiquitin–protein ligases CBL and CBLB were determined in primary T cells after TCR stimulation. Analysis of correlations in protein association as a function of time reveals the importance of the CD5 transmembrane receptor in the regulation of ubiquitylation. © The Author(s) 2016 |
| abstractGer |
Abstract T‐cell receptor (TCR) signaling is essential for the function of T cells and negatively regulated by the E3 ubiquitin–protein ligases CBL and CBLB. Here, we combined mouse genetics and affinity purification coupled to quantitative mass spectrometry to monitor the dynamics of the CBL and CBLB signaling complexes that assemble in normal T cells over 600 seconds of TCR stimulation. We identify most previously known CBL and CBLB interacting partners, as well as a majority of proteins that have not yet been implicated in those signaling complexes. We exploit correlations in protein association with CBL and CBLB as a function of time of TCR stimulation for predicting the occurrence of direct physical association between them. By combining co‐recruitment analysis with biochemical analysis, we demonstrated that the CD5 transmembrane receptor constitutes a key scaffold for CBL‐ and CBLB‐mediated ubiquitylation following TCR engagement. Our results offer an integrated view of the CBL and CBLB signaling complexes induced by TCR stimulation and provide a molecular basis for their negative regulatory function in normal T cells. Synopsis The composition and dynamics of the signalosomes operated by the E3 ubiquitin–protein ligases CBL and CBLB were determined in primary T cells after TCR stimulation. Analysis of correlations in protein association as a function of time reveals the importance of the CD5 transmembrane receptor in the regulation of ubiquitylation. The signalosomes of the E3‐ubiquitin ligases CBL and CBLB exhibit both redundant and distinct features in mature $ CD4^{+} $ T cells.Analysis of correlations in protein association with CBL and CBLB accurately predicts interactions between recruited proteins.CD5 is identified as a key regulator of CBL‐ and CBLB‐mediated ubiquitylation following TCR engagement. Graphical Abstract The composition and dynamics of the signalosomes operated by the E3 ubiquitin–protein ligases CBL and CBLB were determined in primary T cells after TCR stimulation. Analysis of correlations in protein association as a function of time reveals the importance of the CD5 transmembrane receptor in the regulation of ubiquitylation. © The Author(s) 2016 |
| abstract_unstemmed |
Abstract T‐cell receptor (TCR) signaling is essential for the function of T cells and negatively regulated by the E3 ubiquitin–protein ligases CBL and CBLB. Here, we combined mouse genetics and affinity purification coupled to quantitative mass spectrometry to monitor the dynamics of the CBL and CBLB signaling complexes that assemble in normal T cells over 600 seconds of TCR stimulation. We identify most previously known CBL and CBLB interacting partners, as well as a majority of proteins that have not yet been implicated in those signaling complexes. We exploit correlations in protein association with CBL and CBLB as a function of time of TCR stimulation for predicting the occurrence of direct physical association between them. By combining co‐recruitment analysis with biochemical analysis, we demonstrated that the CD5 transmembrane receptor constitutes a key scaffold for CBL‐ and CBLB‐mediated ubiquitylation following TCR engagement. Our results offer an integrated view of the CBL and CBLB signaling complexes induced by TCR stimulation and provide a molecular basis for their negative regulatory function in normal T cells. Synopsis The composition and dynamics of the signalosomes operated by the E3 ubiquitin–protein ligases CBL and CBLB were determined in primary T cells after TCR stimulation. Analysis of correlations in protein association as a function of time reveals the importance of the CD5 transmembrane receptor in the regulation of ubiquitylation. The signalosomes of the E3‐ubiquitin ligases CBL and CBLB exhibit both redundant and distinct features in mature $ CD4^{+} $ T cells.Analysis of correlations in protein association with CBL and CBLB accurately predicts interactions between recruited proteins.CD5 is identified as a key regulator of CBL‐ and CBLB‐mediated ubiquitylation following TCR engagement. Graphical Abstract The composition and dynamics of the signalosomes operated by the E3 ubiquitin–protein ligases CBL and CBLB were determined in primary T cells after TCR stimulation. Analysis of correlations in protein association as a function of time reveals the importance of the CD5 transmembrane receptor in the regulation of ubiquitylation. © The Author(s) 2016 |
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| title_short |
Co‐recruitment analysis of the CBL and CBLB signalosomes in primary T cells identifies CD5 as a key regulator of TCR‐induced ubiquitylation |
| url |
https://dx.doi.org/10.15252/msb.20166837 |
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| author2 |
García‐Blesa, Antonio Chaoui, Karima Fiore, Frédéric Bergot, Elise Girard, Laura Malissen, Marie Burlet‐Schiltz, Odile Gonzalez de Peredo, Anne Malissen, Bernard Roncagalli, Romain |
| author2Str |
García‐Blesa, Antonio Chaoui, Karima Fiore, Frédéric Bergot, Elise Girard, Laura Malissen, Marie Burlet‐Schiltz, Odile Gonzalez de Peredo, Anne Malissen, Bernard Roncagalli, Romain |
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| doi_str |
10.15252/msb.20166837 |
| up_date |
2024-10-25T04:56:06.556Z |
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| score |
7.4001417 |