Advances in methods for native expression and purification of RNA for structural studies
Highlights • Denaturing RNA purification protocols can be unsuitable for various reasons. • RNA can be natively expressed in E. coli using a stabilizing tRNA scaffold. • A series of RNA affinity tags have been developed for native purification. • Simple gel filtration and ion exchange protocols have...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
Batey, Robert T [verfasserIn] |
|---|
| Format: |
E-Artikel |
|---|---|
| Sprache: |
Englisch |
| Erschienen: |
2014 |
|---|
| Umfang: |
8 |
|---|
| Übergeordnetes Werk: |
Enthalten in: Coprocessing of Stainless-Steel Pickling Sludge with Laterite Ore via Rotary Kiln-Electric Furnace Route: Enhanced Desulfurization and Metal Recovery - Li, Guanghui ELSEVIER, 2020, review of all advances : evaluation of key references : comprehensive listing of papers, Amsterdam [u.a.] |
|---|---|
| Übergeordnetes Werk: |
volume:26 ; year:2014 ; pages:1-8 ; extent:8 |
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| DOI / URN: |
10.1016/j.sbi.2014.01.014 |
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Highlights • Denaturing RNA purification protocols can be unsuitable for various reasons. • RNA can be natively expressed in E. coli using a stabilizing tRNA scaffold. • A series of RNA affinity tags have been developed for native purification. • Simple gel filtration and ion exchange protocols have been established for RNA. |
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Highlights • Denaturing RNA purification protocols can be unsuitable for various reasons. • RNA can be natively expressed in E. coli using a stabilizing tRNA scaffold. • A series of RNA affinity tags have been developed for native purification. • Simple gel filtration and ion exchange protocols have been established for RNA. |
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